mRNA_M-pyrifera_M_contig77074.18167.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig77074.18167.1
Unique NamemRNA_M-pyrifera_M_contig77074.18167.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig77074.18167.1 vs. uniprot
Match: A0A812Z384_9DINO (BGAL5 protein (Fragment) n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A812Z384_9DINO)

HSP 1 Score: 108 bits (270), Expect = 9.450e-24
Identity = 82/228 (35.96%), Postives = 111/228 (48.68%), Query Frame = 1
Query:    1 AFEIAAWFARGGSHVNYYMFAPLLNHGTWAGASVTQAYAMDAPICPDGLPHPAKAQHLSNLHASLSSSSSALPATSSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSRVTFLESDATVAVL-VQFEGRNYTLQPSSVLVVGP-DGSVEYDTGTVVPASFHRQVSSPSPL---SFSHWLEPLTSSSTSHLPSRQYSPAPDQGVANGGIGD-----YVVYQTQGAL 654
            A+    WFARGGSHVNYYM+    N G WAGA +T AY++DAP+CPD LPHP KA H + +H++L+  +  L A  +                                   V FLE+      L V + GR Y +QP SVLVV    G+V +DT  V   + HR VSSP P    ++  W EP+       LP+   S   +QG A+  +GD     +++Y    AL
Sbjct:  154 AYHTLRWFARGGSHVNYYMWTGGNNVGHWAGAGITNAYSLDAPLCPDLLPHPTKAAHFTAMHSTLAHIAPVLLAAPAQTGKQFTVPYFDGKAFVNGTQQLGWRYPSEQGHGGVVFLENQFMGGELYVLYGGRKYPMQPQSVLVVDEASGNVLFDTADVQTPATHR-VSSPCPDCLGAWQAWQEPVLGGPP--LPNVTSSTPLEQGNASVQLGDAAFNAFMLYDVDVAL 378          
BLAST of mRNA_M-pyrifera_M_contig77074.18167.1 vs. uniprot
Match: A0A8J6C2K9_DIALT (Glyco_hydro_35 domain-containing protein n=2 Tax=Diacronema lutheri TaxID=2081491 RepID=A0A8J6C2K9_DIALT)

HSP 1 Score: 74.7 bits (182), Expect = 1.010e-11
Identity = 37/66 (56.06%), Postives = 46/66 (69.70%), Query Frame = 1
Query:   19 WFARGGSHVNYYMFAPLLNHGTWAGASVTQAYAMDAPICPDGLPHPAKAQHLSNLHASLSSSSSAL 216
            WFARGGS +NYYMFA     G WAGA++T AYA D P+C DGL H AK   L  +HA+L+S++  L
Sbjct:  302 WFARGGSLINYYMFAGGSTRGLWAGAALTTAYAPDVPVCHDGLRHGAKFHFLQLMHAALASAAPEL 367          
BLAST of mRNA_M-pyrifera_M_contig77074.18167.1 vs. uniprot
Match: A0A1E7F566_9STRA (Putative beta-galactosidase n=1 Tax=Fragilariopsis cylindrus CCMP1102 TaxID=635003 RepID=A0A1E7F566_9STRA)

HSP 1 Score: 70.9 bits (172), Expect = 2.060e-10
Identity = 59/203 (29.06%), Postives = 86/203 (42.36%), Query Frame = 1
Query:    7 EIAAWFARGGSHVNYYMFAPLLNHGTWAGASVTQAYAMDAPICPDGLPHPAKAQHLSNLHASLSSSSSALPAT-------------SSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSRVTFLESDATVAVLVQFEGRN--------YTLQPSSVLVVGPDGSVEYDTGTVVPAS--FHRQV----SSPSPLSFSHWLE 534
            E   WFARGG+H+NYYMF    N G  A   ++  YA DA +C  G  H  K  H  +LH  ++S +S L A              S                                   +VTF+E+DA+   +++    N        Y+L P+S ++V  DG +E+D+  + P S  F R+     S P+ LS+S W E
Sbjct:  379 EALRWFARGGTHLNYYMFFGGYNRGRQAAGGISNQYATDAALCSSGQRHQPKFNHYQSLHKMIASIASTLLAAETALGNEKPVEVMSKEKEWVIGSEQRMFEYFVNQKETGSTVPVRSKSFKQVTFVENDASEEAVIRIPNINEKAKKYQQYSLAPTSAILV-VDGVLEFDSAIIDPKSMSFKREFADSSSIPALLSWSSWPE 580          
BLAST of mRNA_M-pyrifera_M_contig77074.18167.1 vs. uniprot
Match: A0A7R9ZKE6_9STRA (Hypothetical protein n=1 Tax=Craspedostauros australis TaxID=1486917 RepID=A0A7R9ZKE6_9STRA)

HSP 1 Score: 65.1 bits (157), Expect = 1.650e-8
Identity = 70/264 (26.52%), Postives = 109/264 (41.29%), Query Frame = 1
Query:    7 EIAAWFARGGSHVNYYMFAPLLNHGTWAGASVTQAYAMDAPICPDGLPHPAKAQHLSNLHASLSSSSSALPATSSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXS----------------RVTFLESDATVAVLVQFEGRN-YTLQPSSVLVVGPDGSVEYDTGTVV-PASFHRQV---SSPSPLSFSHWLEPLTSSSTSHLPSRQYSPAPDQG--VANGGIGDYVVYQTQGALDGGGDVA---IGATQSTAFVVVVDG 720
            E+  WFARGG+H+NYYM+    N G  A A ++  YA D  +CP G  H  K ++L +LH  L++ + AL    S                                 +                 V F+E+DA   V     G    +L P+SV V+  DG+VE+D+  +   A  ++++    + S LS++   EPL  S      +    P       +A+    DY  Y+T   L+   D A   I   ++ +FV  +DG
Sbjct:   72 EVLKWFARGGAHLNYYMWWGGYNRGRSAAAGISNMYAADGVLCPSGQRHHPKFEYLQSLHDMLATIAPALLHRDSALHQDKDLLVKNSNGEWALQGDQRMFEYGAQNTNGPGKDGDGGSENGSLPHVIFVENDANFPVTAWVYGYGAVSLSPASVTVI-VDGAVEFDSAIIDGQAEQYQRIFDEDTASELSWTTQPEPLRLSEKDKKVTASTEPIEQTALNIASDVSSDYAWYETNITLNADMDDATLHIDTAEANSFVAYIDG 334          
BLAST of mRNA_M-pyrifera_M_contig77074.18167.1 vs. uniprot
Match: A0A7S3K0E4_9STRA (Hypothetical protein n=1 Tax=Aureoumbra lagunensis TaxID=44058 RepID=A0A7S3K0E4_9STRA)

HSP 1 Score: 65.1 bits (157), Expect = 1.800e-8
Identity = 31/65 (47.69%), Postives = 41/65 (63.08%), Query Frame = 1
Query:    1 AFEIAAWFARGGSHVNYYMFAPLLNHGTWAGASVTQAYAMDAPICPDGLPHPAKAQHLSNLHASL 195
            +++   WF RGGSHVN YM+A   N G  A A +TQAYA DA IC +G+PH  K  H + + + L
Sbjct:  334 SYQALRWFERGGSHVNLYMWAGGSNFGPSAAAGITQAYAFDAAICANGIPHEPKFDHFAAMFSVL 398          
BLAST of mRNA_M-pyrifera_M_contig77074.18167.1 vs. uniprot
Match: A0A7S2ESK3_9STRA (Hypothetical protein n=1 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A7S2ESK3_9STRA)

HSP 1 Score: 59.3 bits (142), Expect = 1.030e-6
Identity = 26/63 (41.27%), Postives = 38/63 (60.32%), Query Frame = 1
Query:    7 EIAAWFARGGSHVNYYMFAPLLNHGTWAGASVTQAYAMDAPICPDGLPHPAKAQHLSNLHASL 195
            ++  WFARGGSH+N+YMF    N    +   +  AY+++AP+CP+G  H  K  HL   H+ L
Sbjct:  149 DVMRWFARGGSHINHYMFWGGYNRERMSAGGIMNAYSLEAPLCPNGQRHYPKFDHLRLCHSLL 211          
BLAST of mRNA_M-pyrifera_M_contig77074.18167.1 vs. uniprot
Match: A0A7S1ZWI8_9STRA (Hypothetical protein n=1 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A7S1ZWI8_9STRA)

HSP 1 Score: 59.3 bits (142), Expect = 1.500e-6
Identity = 26/63 (41.27%), Postives = 38/63 (60.32%), Query Frame = 1
Query:    7 EIAAWFARGGSHVNYYMFAPLLNHGTWAGASVTQAYAMDAPICPDGLPHPAKAQHLSNLHASL 195
            ++  WFARGGSH+N+YMF    N    +   +  AY+++AP+CP+G  H  K  HL   H+ L
Sbjct:   75 DVMRWFARGGSHINHYMFWGGYNRERMSAGGIMNAYSLEAPLCPNGQRHYPKFDHLRLCHSLL 137          
BLAST of mRNA_M-pyrifera_M_contig77074.18167.1 vs. uniprot
Match: B7FYH5_PHATC (Beta-galactosidase n=1 Tax=Phaeodactylum tricornutum (strain CCAP 1055/1) TaxID=556484 RepID=B7FYH5_PHATC)

HSP 1 Score: 57.4 bits (137), Expect = 7.230e-6
Identity = 66/258 (25.58%), Postives = 99/258 (38.37%), Query Frame = 1
Query:    1 AFEIAAWFARGGSHVNYYMFAPLLNHGTWAGASVTQAYAMDAPICPDGLPHPAKAQHLSNLHASLSSSSSAL--PATSSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSRVTFLESDATVAVLVQFEGRN------YTLQP-SSVLVVGPDGSVEYDTGTV-VPASFHRQVSSPSPLSFSH---WLEPLTSSSTSHLPSRQYSPAPDQGVANGGI--GDYVVYQTQGALD---GGGDVAIGATQSTAFVVVVDG 720
            A +   WFARGG+H+NYYM+    N G  + A +  AYA DA +C  G     K  H   LH  ++  ++ L    TS                                   +V FLE+DA    + +  G        + ++P SS +V+  DG V +D+ T+   A   R+     P    H   W EP+  + T         P     + +      DY  Y T   +D       + IG  ++TA  V +DG
Sbjct:  467 ATDALQWFARGGTHLNYYMWWGGYNRGRSSAAGIMNAYATDAFLCSSGQRRHPKYDHFLALHLVIADIAAILLHAPTSLLKNASVEIMDGDDWIVGDNQRQFLYQVLDTHDSKQVIFLENDANTTEMARLTGAKADDSLVFVMKPYSSQIVI--DGIVAFDSSTISTKAMSFRRTLHYEPAVLLHLTSWSEPIAGADTDQNAHVSTEPLEQTNLNSKASISSDYAWYGTDVKIDVVLSQVKLYIGTEKATALAVFIDG 722          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig77074.18167.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 8
Match NameE-valueIdentityDescription
A0A812Z384_9DINO9.450e-2435.96BGAL5 protein (Fragment) n=1 Tax=Symbiodinium sp. ... [more]
A0A8J6C2K9_DIALT1.010e-1156.06Glyco_hydro_35 domain-containing protein n=2 Tax=D... [more]
A0A1E7F566_9STRA2.060e-1029.06Putative beta-galactosidase n=1 Tax=Fragilariopsis... [more]
A0A7R9ZKE6_9STRA1.650e-826.52Hypothetical protein n=1 Tax=Craspedostauros austr... [more]
A0A7S3K0E4_9STRA1.800e-847.69Hypothetical protein n=1 Tax=Aureoumbra lagunensis... [more]
A0A7S2ESK3_9STRA1.030e-641.27Hypothetical protein n=1 Tax=Ditylum brightwellii ... [more]
A0A7S1ZWI8_9STRA1.500e-641.27Hypothetical protein n=1 Tax=Ditylum brightwellii ... [more]
B7FYH5_PHATC7.230e-625.58Beta-galactosidase n=1 Tax=Phaeodactylum tricornut... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig77074contigM-pyrifera_M_contig77074:94..990 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score78.6
Seed ortholog evalue4.5e-12
Seed eggNOG ortholog4792.ETI50934
Preferred nameGLB1L
KEGG rclassRC00002,RC00010,RC00043,RC00049,RC00064,RC00452,RC00632,RC02750,RC02798,RC02850,RC03314
KEGG koko:K01190,ko:K11540,ko:K12308,ko:K12309,ko:K14297,ko:K22390
KEGG TC1.I.1
KEGG ReactionR00256,R00575,R01105,R01395,R01397,R01678,R01993,R03355,R04633,R04783,R06010,R06114,R07807,R10948,R10949
KEGG Pathwayko00052,ko00240,ko00250,ko00511,ko00531,ko00600,ko00604,ko01100,ko03013,ko04142,ko05164,map00052,map00240,map00250,map00511,map00531,map00600,map00604,map01100,map03013,map04142,map05164
KEGG ModuleM00051,M00079,M00427
Hectar predicted targeting categoryother localisation
GOsGO:0000323,GO:0001775,GO:0002252,GO:0002263,GO:0002274,GO:0002275,GO:0002283,GO:0002366,GO:0002376,GO:0002443,GO:0002444,GO:0002446,GO:0003674,GO:0003824,GO:0004308,GO:0004553,GO:0004565,GO:0005488,GO:0005515,GO:0005575,GO:0005576,GO:0005615,GO:0005622,GO:0005623,GO:0005737,GO:0005764,GO:0005766,GO:0005773,GO:0005775,GO:0005794,GO:0005975,GO:0005996,GO:0006012,GO:0006022,GO:0006026,GO:0006027,GO:0006082,GO:0006629,GO:0006643,GO:0006664,GO:0006665,GO:0006687,GO:0006790,GO:0006807,GO:0006810,GO:0006887,GO:0006955,GO:0008150,GO:0008152,GO:0009056,GO:0009057,GO:0009987,GO:0012505,GO:0015925,GO:0016052,GO:0016192,GO:0016787,GO:0016798,GO:0016936,GO:0016997,GO:0019318,GO:0019320,GO:0019388,GO:0030141,GO:0030203,GO:0031410,GO:0031974,GO:0031982,GO:0031983,GO:0032940,GO:0034774,GO:0035578,GO:0036230,GO:0042119,GO:0042339,GO:0042340,GO:0042582,GO:0042802,GO:0042803,GO:0043170,GO:0043202,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043299,GO:0043312,GO:0043436,GO:0044237,GO:0044238,GO:0044248,GO:0044255,GO:0044262,GO:0044273,GO:0044281,GO:0044282,GO:0044421,GO:0044422,GO:0044424,GO:0044433,GO:0044437,GO:0044444,GO:0044446,GO:0044464,GO:0045055,GO:0045321,GO:0046365,GO:0046903,GO:0046983,GO:0050896,GO:0051179,GO:0051234,GO:0060205,GO:0070013,GO:0071704,GO:0097367,GO:0097708,GO:0099503,GO:0101002,GO:1901135,GO:1901136,GO:1901564,GO:1901565,GO:1901575,GO:1903509,GO:1903510,GO:1904813
EggNOG free text desc.beta-galactosidase activity
EggNOG OGs3Q8TS@4776,COG1874@1,KOG0496@2759
EC2.1.3.2,3.2.1.23,3.5.2.3,6.3.5.5
COG Functional cat.G
Best tax levelPeronosporales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko01002,ko03019,ko03036,ko04147
Exons2
Model size720
Cds size720
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816671.7067573-CDS-M-pyrifera_M_contig77074:93..6241622816671.7067573-CDS-M-pyrifera_M_contig77074:93..624Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig77074 94..624 -
1692278110.5606112-CDS-M-pyrifera_M_contig77074:93..6241692278110.5606112-CDS-M-pyrifera_M_contig77074:93..624Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig77074 94..624 -
1622816671.7188785-CDS-M-pyrifera_M_contig77074:801..9901622816671.7188785-CDS-M-pyrifera_M_contig77074:801..990Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig77074 802..990 -
1692278110.5720437-CDS-M-pyrifera_M_contig77074:801..9901692278110.5720437-CDS-M-pyrifera_M_contig77074:801..990Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig77074 802..990 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig77074.18167.1prot_M-pyrifera_M_contig77074.18167.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig77074 94..990 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig77074.18167.1

>prot_M-pyrifera_M_contig77074.18167.1 ID=prot_M-pyrifera_M_contig77074.18167.1|Name=mRNA_M-pyrifera_M_contig77074.18167.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=240bp
AFEIAAWFARGGSHVNYYMFAPLLNHGTWAGASVTQAYAMDAPICPDGLP
HPAKAQHLSNLHASLSSSSSALPATSSSASSPSSSSSSSSSSSFSSSSSP
SSSSPSSPSPSRVTFLESDATVAVLVQFEGRNYTLQPSSVLVVGPDGSVE
YDTGTVVPASFHRQVSSPSPLSFSHWLEPLTSSSTSHLPSRQYSPAPDQG
VANGGIGDYVVYQTQGALDGGGDVAIGATQSTAFVVVVDG
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mRNA from alignment at M-pyrifera_M_contig77074:94..990-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig77074.18167.1 ID=mRNA_M-pyrifera_M_contig77074.18167.1|Name=mRNA_M-pyrifera_M_contig77074.18167.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=897bp|location=Sequence derived from alignment at M-pyrifera_M_contig77074:94..990- (Macrocystis pyrifera P11B4 male)
GCCTTTGAAATCGCAGCTTGGTTCGCCAGAGGGGGATCGCACGTGAACTA CTACATGTTCGCCCCTCTCCTCAACCACGGCACATGGGCAGGCGCTTCTG TGACACAAGCTTACGCCATGGATGCGCCAATCTGCCCCGACGGACTCCCA CACCCCGCAAAGGCCCAACACCTCTCCAACCTCCATGCTGTCCTGGCCAA ATACGCCCCCACAATCATCAACAGCCCGCCGCAACTCCACCACTCCTTCG TCGTCCCCTGGTTCAACAACAAGACGCAAAAGTTTGAAAACGGAACGCAA CAAATCGGATTCACCTATCAAGTGTCCAGCCGAGCAAGTGCAGAAAGAGG AACAAGGGGGTCTGAGTCATTGTCTTCTTCTTCTTCTGCATTACCAGCAA CATCATCTTCAGCATCTTCACCTTCATCATCTTCATCTTCATCTTCATCT TCATCATTTTCATCCTCATCATCTCCATCCTCCTCCTCCCCATCCTCCCC TTCTCCTTCTCGAGTGACTTTTTTGGAGAGTGACGCTACCGTTGCGGTGC TGGTGCAGTTTGAGGGTCGGAACTACACTTTGCAGCCGTCTTCTGTGCTG GTGGTGGGGCCAGATGGGTCTGTGGAGTATGACACTGGGACGGTGGTACC AGCTTCGTTCCATCGCCAAGTCTCTTCTCCTTCTCCGCTCTCCTTTTCCC ATTGGCTTGAACCCCTCACTTCCTCCTCCACGTCCCACCTCCCTTCTCGG CAGTATTCGCCTGCTCCTGACCAAGGAGTGGCCAATGGAGGAATTGGGGA CTATGTGGTGTACCAGACCCAGGGTGCGTTGGACGGGGGTGGAGACGTGG CAATTGGAGCAACTCAATCGACTGCATTCGTCGTGGTGGTGGATGGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig77074:94..990-

>mRNA_M-pyrifera_M_contig77074.18167.1 ID=mRNA_M-pyrifera_M_contig77074.18167.1|Name=mRNA_M-pyrifera_M_contig77074.18167.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1440bp|location=Sequence derived from alignment at M-pyrifera_M_contig77074:94..990- (Macrocystis pyrifera P11B4 male)
GCCTTTGAAATCGCAGCTTGGTTCGCCAGAGGGGGATCGCACGTGAACTA
CTACATGTTCGCCCCTCTCCTCAACCACGGCACATGGGCAGGCGCTTCTG
TGACACAAGCTTACGCCATGGATGCGCCAATCTGCCCCGACGGACTCCCA
CACCCCGCAAAGGCCCAACACCTCTCCAACCTCCATGCTGCCTTTGAAAT
CGCAGCTTGGTTCGCCAGAGGGGGATCGCACGTGAACTACTACATGTTCG
CCCCTCTCCTCAACCACGGCACATGGGCAGGCGCTTCTGTGACACAAGCT
TACGCCATGGATGCGCCAATCTGCCCCGACGGACTCCCACACCCCGCAAA
GGCCCAACACCTCTCCAACCTCCATGCTTCATTGTCTTCTTCTTCTTCTG
CATTACCAGCAACATCATCTTCAGCATCTTCACCTTCATCATCTTCATCT
TCATCTTCATCTTCATCATTTTCATCCTCATCATCTCCATCCTCCTCCTC
CCCATCCTCCCCTTCTCCTTCTCGAGTGACTTTTTTGGAGAGTGACGCTA
CCGTTGCGGTGCTGGTGCAGTTTGAGGGTCGGAACTACACTTTGCAGCCG
TCTTCTGTGCTGGTGGTGGGGCCAGATGGGTCTGTGGAGTATGACACTGG
GACGGTGGTACCAGCTTCGTTCCATCGCCAAGTCTCTTCTCCTTCTCCGC
TCTCCTTTTCCCATTGGCTTGAACCCCTCACTTCCTCCTCCACGTCCCAC
CTCCCTTCTCGGCAGTATTCGCCTGCTCCTGACCAAGGAGTGGCCAATGG
AGGAATTGGGGACTATGTGGTGTACCAGACCCAGGGTGCGTTGGACGGGG
GTGGAGACGTGGCAATTGGAGCAACTCAATCGACTGCATTCGTCGTGGTG
GTGGATGGATCATTGTCTTCTTCTTCTTCTGCATTACCAGCAACATCATC
TTCAGCATCTTCACCTTCATCATCTTCATCTTCATCTTCATCTTCATCAT
TTTCATCCTCATCATCTCCATCCTCCTCCTCCCCATCCTCCCCTTCTCCT
TCTCGAGTGACTTTTTTGGAGAGTGACGCTACCGTTGCGGTGCTGGTGCA
GTTTGAGGGTCGGAACTACACTTTGCAGCCGTCTTCTGTGCTGGTGGTGG
GGCCAGATGGGTCTGTGGAGTATGACACTGGGACGGTGGTACCAGCTTCG
TTCCATCGCCAAGTCTCTTCTCCTTCTCCGCTCTCCTTTTCCCATTGGCT
TGAACCCCTCACTTCCTCCTCCACGTCCCACCTCCCTTCTCGGCAGTATT
CGCCTGCTCCTGACCAAGGAGTGGCCAATGGAGGAATTGGGGACTATGTG
GTGTACCAGACCCAGGGTGCGTTGGACGGGGGTGGAGACGTGGCAATTGG
AGCAACTCAATCGACTGCATTCGTCGTGGTGGTGGATGGA
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