mRNA_M-pyrifera_M_contig76930.18132.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig76930.18132.1
Unique NamemRNA_M-pyrifera_M_contig76930.18132.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig76930.18132.1 vs. uniprot
Match: A0A0R3U4X9_MESCO (DNA topoisomerase n=2 Tax=Mesocestoides corti TaxID=53468 RepID=A0A0R3U4X9_MESCO)

HSP 1 Score: 169 bits (429), Expect = 2.980e-45
Identity = 90/197 (45.69%), Postives = 121/197 (61.42%), Query Frame = 1
Query:   22 RPFPLNTVALLKLASKVLGMGPQRTMQVAERLYLSGLVSYPRTESTAYPSSFDLRHAISQHTSHPAYGXXXXXXXXSAPTKPRKGHDAGDHPPITPVASPRGGLGGEEGRVYDLICRQFLASVSPDAKFKATTVALSVGKEKATLSARKLISPGFLAILNRSMHVVEEXXXXXXEDPTREEGHWVGSVHEGMVLPIA 612
            RP  LNTV LL++AS  LG+GP  TM +AERLY SG ++YPRTESTAYP SFDL+  + QH +HP +G        S  T+PR GH+AGDHPPI P+ S    L G+ GR+YD + + F+A++ PD K++ TTV  ++G EK T S  +++ PGF  I   +   VEE       DP  +       +H G  LP+A
Sbjct:  293 RPQGLNTVELLRVASSSLGIGPHATMAIAERLYTSGFINYPRTESTAYPPSFDLKDLLRQHANHPKWGDVVSGLLKSGYTQPRAGHNAGDHPPIAPMRSTNE-LSGDAGRIYDYVAQHFIATLMPDCKYETTTVVFAIGDEKFTASCSRVLDPGFTRIF--TWQAVEE-------DPLPD-----SILHPGATLPLA 474          
BLAST of mRNA_M-pyrifera_M_contig76930.18132.1 vs. uniprot
Match: A0A8C2RS83_CAPHI (DNA topoisomerase n=1 Tax=Capra hircus TaxID=9925 RepID=A0A8C2RS83_CAPHI)

HSP 1 Score: 161 bits (408), Expect = 3.120e-45
Identity = 82/166 (49.40%), Postives = 106/166 (63.86%), Query Frame = 1
Query:    4 KEVGVVRPFPLNTVALLKLASKVLGMGPQRTMQVAERLYLSGLVSYPRTESTAYPSSFDLRHAISQHTSHPAYGXXXXXXXXSAPTKPRKGHDAGDHPPITPVASP-RGGLGGEEGRVYDLICRQFLASVSPDAKFKATTVALSVGKEKATLSARKLISPGFLAIL 498
            KE    RP  LNTV +L++AS  LGMGPQ  MQ AERLY  G +SYPRTE+T YP SFDL+  + Q  SHP +             +PRKGHDAGDHPPITP+ S     LGGE  R+Y+ I R F+A+VS D ++  ++V+  +G E+ T S + +ISPGF  I+
Sbjct:   20 KEKAKQRPLALNTVEMLRVASSALGMGPQHAMQTAERLYTQGYISYPRTETTHYPESFDLKGPLRQQASHPYWADTVKRLLAEGLNRPRKGHDAGDHPPITPMKSATEAELGGEAWRLYEYITRHFIATVSHDCRYLQSSVSFRIGPERFTCSGKTVISPGFTEIM 185          
BLAST of mRNA_M-pyrifera_M_contig76930.18132.1 vs. uniprot
Match: A0A1Z5JKM3_FISSO (DNA topoisomerase n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5JKM3_FISSO)

HSP 1 Score: 167 bits (424), Expect = 1.950e-44
Identity = 89/163 (54.60%), Postives = 112/163 (68.71%), Query Frame = 1
Query:   22 RPFPLNTVALLKLASKVLGMGPQRTMQVAERLYLSGLVSYPRTESTAYPSSFDLRHAISQHTSHPAYGXXXXXXXXSAPTKPRKGHDAGDHPPITPVASPRGGLGGEEGRVYDLICRQFLASVSPDAKFKATTVALSVG----KEKATLSARKLISPGFLAIL 498
            RP PLNTVALLK  SK LG+GP   MQ+AERLYLSG +SYPRTESTAYP SFD+   + Q TS PA+G        +  T+PR G D GDHPPIT + S  G L G+  RVY+L+ R F+A+VSPDA +++T V  S+     K   TLS ++++SPGFLA+L
Sbjct:  503 RPTPLNTVALLKACSKALGIGPHSAMQIAERLYLSGYLSYPRTESTAYPKSFDINGTLQQQTSEPAWGAYVRELLQTGVTRPRGGVDMGDHPPITIMRSGLG-LSGDMARVYELVARHFIATVSPDAVWRSTRVDFSIAALGDKGHFTLSGKEIVSPGFLAVL 664          
BLAST of mRNA_M-pyrifera_M_contig76930.18132.1 vs. uniprot
Match: A0A0G4FZK5_VITBC (DNA topoisomerase n=2 Tax=Vitrella brassicaformis TaxID=1169539 RepID=A0A0G4FZK5_VITBC)

HSP 1 Score: 167 bits (423), Expect = 2.130e-44
Identity = 87/167 (52.10%), Postives = 111/167 (66.47%), Query Frame = 1
Query:    1 EKEVGVVRPFPLNTVALLKLASKVLGMGPQRTMQVAERLYLSGLVSYPRTESTAYPSSFDLRHAISQHTSHPAYGXXXXXXXXSAPTKPRKGHDAGDHPPITPV-ASPRGGLGGEEG-RVYDLICRQFLASVSPDAKFKATTVALSVGKEKATLSARKLISPGFLAI 495
            EKE    RP  LNTVALLK AS  LG+GP + MQVAERLYLSG ++YPRTE+  YP +FDLR   ++ +SHP +G            KPR+GHDAGDHPPITPV  +  G +GG +  RVY+L+ R FLA+VSPD KF  T V   +  E+ +++ R+LI PGF  +
Sbjct:  289 EKEERRARPTALNTVALLKAASSGLGIGPHQAMQVAERLYLSGYITYPRTETNQYPPNFDLRKTAAEQSSHPMWGAYVRELLQKGLAKPREGHDAGDHPPITPVRCATEGDVGGGDAWRVYELVTRHFLATVSPDCKFWRTRVRFDINGERFSVAGRRLIDPGFTGV 455          
BLAST of mRNA_M-pyrifera_M_contig76930.18132.1 vs. uniprot
Match: B4E3G2_HUMAN (DNA topoisomerase n=1 Tax=Homo sapiens TaxID=9606 RepID=B4E3G2_HUMAN)

HSP 1 Score: 157 bits (398), Expect = 3.250e-44
Identity = 78/166 (46.99%), Postives = 106/166 (63.86%), Query Frame = 1
Query:    4 KEVGVVRPFPLNTVALLKLASKVLGMGPQRTMQVAERLYLSGLVSYPRTESTAYPSSFDLRHAISQHTSHPAYGXXXXXXXXSAPTKPRKGHDAGDHPPITPVASP-RGGLGGEEGRVYDLICRQFLASVSPDAKFKATTVALSVGKEKATLSARKLISPGFLAIL 498
            KE    RP  LNTV +L++AS  LGMGPQ  MQ AERLY  G +SYPRTE+T YP +FDL+ ++ Q  +HP +             +PRKGHDAGDHPPITP+ S     LGG+  R+Y+ I R F+A+VS D K+  +T++  +G E  T S + ++SPGF  ++
Sbjct:   20 KEKAKQRPLALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYPENFDLKGSLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIATVSHDCKYLQSTISFRIGPELFTCSGKTVLSPGFTEVM 185          
BLAST of mRNA_M-pyrifera_M_contig76930.18132.1 vs. uniprot
Match: A7RW77_NEMVE (DNA topoisomerase n=1 Tax=Nematostella vectensis TaxID=45351 RepID=A7RW77_NEMVE)

HSP 1 Score: 165 bits (418), Expect = 1.010e-43
Identity = 82/160 (51.25%), Postives = 107/160 (66.88%), Query Frame = 1
Query:   22 RPFPLNTVALLKLASKVLGMGPQRTMQVAERLYLSGLVSYPRTESTAYPSSFDLRHAISQHTSHPAYGXXXXXXXXSAPTKPRKGHDAGDHPPITPV-ASPRGGLGGEEGRVYDLICRQFLASVSPDAKFKATTVALSVGKEKATLSARKLISPGFLAIL 498
            RP  LNTV +L+LAS  LGMGPQ TMQ+AERLY  G +SYPRTE+T YP +FDL+ A+ Q +    +G         A TKPRKGHDAGDHPPITP+  +    LGG+  R+YD I R FLA++SPD K+  TTV   +GKE  + + + +  PGF +++
Sbjct:  295 RPQALNTVEMLRLASSGLGMGPQHTMQIAERLYTQGYISYPRTETTHYPENFDLKGALKQQSGSAMWGSHVRELLDGAMTKPRKGHDAGDHPPITPMRCASESELGGDPWRLYDFITRHFLATLSPDCKYLQTTVKFKIGKEAFSFTGKAVTDPGFTSVM 454          
BLAST of mRNA_M-pyrifera_M_contig76930.18132.1 vs. uniprot
Match: B1H2R2_XENTR (DNA topoisomerase n=1 Tax=Xenopus tropicalis TaxID=8364 RepID=B1H2R2_XENTR)

HSP 1 Score: 160 bits (405), Expect = 2.300e-43
Identity = 81/167 (48.50%), Postives = 110/167 (65.87%), Query Frame = 1
Query:    1 EKEVGVVRPFPLNTVALLKLASKVLGMGPQRTMQVAERLYLSGLVSYPRTESTAYPSSFDLRHAISQHTSHPAYGXXXXXXXXSAPTKPRKGHDAGDHPPITPVASP-RGGLGGEEGRVYDLICRQFLASVSPDAKFKATTVALSVGKEKATLSARKLISPGFLAIL 498
            +KE    RP  LNTV +L++AS  LGMGPQ TMQ+AERLY  G +SYPRTE+T YP +FDL+  + Q  ++P +             +PRKG DAGDHPPITP+ S     LGG+  R+Y+ I R F+A++S D K+  T++A SVG EK T + +++ISPGF  I+
Sbjct:  277 KKEKAKQRPQALNTVEMLRVASSALGMGPQHTMQIAERLYTQGYISYPRTETTHYPENFDLKGTLRQQANNPFWADMVKTLLAEGINRPRKGTDAGDHPPITPMRSATEAELGGDAWRLYEYITRHFIATISHDCKYLQTSIAFSVGPEKFTTTCKEVISPGFTEIM 443          
BLAST of mRNA_M-pyrifera_M_contig76930.18132.1 vs. uniprot
Match: A0A176WE78_MARPO (DNA topoisomerase n=1 Tax=Marchantia polymorpha subsp. ruderalis TaxID=1480154 RepID=A0A176WE78_MARPO)

HSP 1 Score: 163 bits (412), Expect = 2.850e-43
Identity = 85/167 (50.90%), Postives = 109/167 (65.27%), Query Frame = 1
Query:    1 EKEVGVVRPFPLNTVALLKLASKVLGMGPQRTMQVAERLYLSGLVSYPRTESTAYPSSFDLRHAISQHTSHPAYGXXXXXXXXSAPTKPRKGHDAGDHPPITPV-ASPRGGLGGEEGRVYDLICRQFLASVSPDAKFKATTVALSVGKEKATLSARKLISPGFLAIL 498
            +KE   VRP  LNTV +LK+AS  L MGP   MQ+AERLY  G +SYPRTEST+YPSSFD+R A++   +HP +G        S    P+ G DAGDHPPITP+  +    LGG+  R+Y+ + R FL SVSPD KF  T V LS G EK +++ R +ISPGF + +
Sbjct:  126 KKEERKVRPNGLNTVDMLKVASSALNMGPHHAMQIAERLYTQGYISYPRTESTSYPSSFDVRGALALQRNHPIWGDYAQSLIASGSYVPKAGVDAGDHPPITPMRCATEAELGGDAWRLYEYVTRHFLGSVSPDCKFMRTKVLLSAGGEKFSIAGRNVISPGFTSCM 292          
BLAST of mRNA_M-pyrifera_M_contig76930.18132.1 vs. uniprot
Match: A0A4S2MDD2_OPIFE (DNA topoisomerase (Fragment) n=1 Tax=Opisthorchis felineus TaxID=147828 RepID=A0A4S2MDD2_OPIFE)

HSP 1 Score: 157 bits (398), Expect = 3.360e-43
Identity = 85/170 (50.00%), Postives = 111/170 (65.29%), Query Frame = 1
Query:    4 KEVGVVRPFPLNTVALLKLASKVLGMGPQRTMQVAERLYLSGLVSYPRTESTAYPSSFDLRHAISQHTSHPAYGXXXXXXXXSAPTKPRKGHDAGDHPPITPV--ASPRGGLGGEEGRVYDLICRQFLASVSPDAKFKATTVALSVGK-EKATLSARKLISPGFLAILNR 504
            KE    RP  LNTV +L++AS  LG+GP   M VAERLY SG ++YPRTE+T YPS+FDLR  + Q   HP +G        S  T PRKGHDAGDHPPI P+  A+P G LG +  R+Y+ I + FLA+V PD ++++T V LS+G  E  TL+  +++ PGF  IL R
Sbjct:   45 KEKVKPRPQGLNTVEMLRVASACLGIGPHAAMAVAERLYTSGYINYPRTETTTYPSTFDLRGLVQQQAKHPQWGDVARDLLASGLTPPRKGHDAGDHPPIAPMRMATP-GELGHDAARLYEFIAQHFLATVMPDCRYESTQVTLSIGDGELFTLTGIRVLEPGFTRILTR 213          
BLAST of mRNA_M-pyrifera_M_contig76930.18132.1 vs. uniprot
Match: A0A2F0BAR6_ESCRO (DNA topoisomerase (Fragment) n=1 Tax=Eschrichtius robustus TaxID=9764 RepID=A0A2F0BAR6_ESCRO)

HSP 1 Score: 160 bits (406), Expect = 4.230e-43
Identity = 80/166 (48.19%), Postives = 106/166 (63.86%), Query Frame = 1
Query:    4 KEVGVVRPFPLNTVALLKLASKVLGMGPQRTMQVAERLYLSGLVSYPRTESTAYPSSFDLRHAISQHTSHPAYGXXXXXXXXSAPTKPRKGHDAGDHPPITPVASP-RGGLGGEEGRVYDLICRQFLASVSPDAKFKATTVALSVGKEKATLSARKLISPGFLAIL 498
            KE    RP  LNTV +L++AS  LGMGPQ  MQ AERLY  G +SYPRTE+T YP SFDL+  + Q  +HP +             +PRKGHDAGDHPPITP+ S     LGGE  R+Y+ I R F+A++S D K+  +T++  +G E+ T + + +ISPGF  I+
Sbjct:  199 KEKAKQRPLALNTVEMLRMASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYPESFDLKGPLRQQANHPYWADTVKQLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGEAWRLYEYITRHFIATISHDCKYLQSTISFRIGPERFTCTGKTVISPGFTEIM 364          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig76930.18132.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A0R3U4X9_MESCO2.980e-4545.69DNA topoisomerase n=2 Tax=Mesocestoides corti TaxI... [more]
A0A8C2RS83_CAPHI3.120e-4549.40DNA topoisomerase n=1 Tax=Capra hircus TaxID=9925 ... [more]
A0A1Z5JKM3_FISSO1.950e-4454.60DNA topoisomerase n=2 Tax=Fistulifera solaris TaxI... [more]
A0A0G4FZK5_VITBC2.130e-4452.10DNA topoisomerase n=2 Tax=Vitrella brassicaformis ... [more]
B4E3G2_HUMAN3.250e-4446.99DNA topoisomerase n=1 Tax=Homo sapiens TaxID=9606 ... [more]
A7RW77_NEMVE1.010e-4351.25DNA topoisomerase n=1 Tax=Nematostella vectensis T... [more]
B1H2R2_XENTR2.300e-4348.50DNA topoisomerase n=1 Tax=Xenopus tropicalis TaxID... [more]
A0A176WE78_MARPO2.850e-4350.90DNA topoisomerase n=1 Tax=Marchantia polymorpha su... [more]
A0A4S2MDD2_OPIFE3.360e-4350.00DNA topoisomerase (Fragment) n=1 Tax=Opisthorchis ... [more]
A0A2F0BAR6_ESCRO4.230e-4348.19DNA topoisomerase (Fragment) n=1 Tax=Eschrichtius ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig76930contigM-pyrifera_M_contig76930:4..618 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeMetazoa
Seed ortholog score166.4
Seed ortholog evalue1.4e-38
Seed eggNOG ortholog45351.EDO44262
Preferred nameTOP3B
KEGG koko:K03165,ko:K08466
KEGG Pathwayko03440,ko03460,map03440,map03460
KEGG ModuleM00414
Hectar predicted targeting categoryother localisation
GOsGO:0000793,GO:0003674,GO:0003676,GO:0003677,GO:0003824,GO:0003916,GO:0003917,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005694,GO:0006139,GO:0006259,GO:0006265,GO:0006725,GO:0006807,GO:0006996,GO:0007059,GO:0008150,GO:0008152,GO:0009987,GO:0016043,GO:0016853,GO:0034641,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0044087,GO:0044089,GO:0044237,GO:0044238,GO:0044260,GO:0044424,GO:0044464,GO:0046483,GO:0048518,GO:0048522,GO:0050789,GO:0050793,GO:0050794,GO:0050803,GO:0050807,GO:0051094,GO:0051128,GO:0051130,GO:0051239,GO:0051240,GO:0051276,GO:0051960,GO:0051962,GO:0051963,GO:0051965,GO:0065007,GO:0065008,GO:0071103,GO:0071704,GO:0071840,GO:0090304,GO:0097159,GO:0140097,GO:1901360,GO:1901363,GO:2000026
EggNOG free text desc.DNA topoisomerase type I activity
EggNOG OGs3AFA5@33154,3B983@33208,COG0550@1,KOG1957@2759
EC5.99.1.2
COG Functional cat.L
Best tax levelMetazoa
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko03032,ko03400,ko04030
Exons1
Model size615
Cds size615
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816669.4544015-CDS-M-pyrifera_M_contig76930:3..6181622816669.4544015-CDS-M-pyrifera_M_contig76930:3..618Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig76930 4..618 +
1692278109.1430387-CDS-M-pyrifera_M_contig76930:3..6181692278109.1430387-CDS-M-pyrifera_M_contig76930:3..618Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig76930 4..618 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig76930.18132.1prot_M-pyrifera_M_contig76930.18132.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig76930 4..618 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig76930.18132.1

>prot_M-pyrifera_M_contig76930.18132.1 ID=prot_M-pyrifera_M_contig76930.18132.1|Name=mRNA_M-pyrifera_M_contig76930.18132.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=205bp
EKEVGVVRPFPLNTVALLKLASKVLGMGPQRTMQVAERLYLSGLVSYPRT
ESTAYPSSFDLRHAISQHTSHPAYGAYAASLLSSAPTKPRKGHDAGDHPP
ITPVASPRGGLGGEEGRVYDLICRQFLASVSPDAKFKATTVALSVGKEKA
TLSARKLISPGFLAILNRSMHVVEEEEGGGGEDPTREEGHWVGSVHEGMV
LPIAS
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mRNA from alignment at M-pyrifera_M_contig76930:4..618+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig76930.18132.1 ID=mRNA_M-pyrifera_M_contig76930.18132.1|Name=mRNA_M-pyrifera_M_contig76930.18132.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=615bp|location=Sequence derived from alignment at M-pyrifera_M_contig76930:4..618+ (Macrocystis pyrifera P11B4 male)
GAGAAGGAAGTTGGGGTGGTGAGGCCTTTTCCTCTGAATACCGTGGCGCT GCTGAAACTGGCCTCCAAAGTGCTGGGGATGGGACCACAACGAACCATGC AAGTGGCGGAACGGCTCTATTTGTCTGGCCTGGTCTCCTACCCCCGCACA GAGTCCACAGCTTACCCTTCCTCCTTCGACCTCAGACACGCCATCTCCCA GCACACCTCCCACCCGGCTTACGGCGCCTATGCCGCCTCCCTCCTCTCCT CTGCCCCCACAAAACCGCGAAAAGGTCATGATGCAGGAGATCACCCCCCC ATCACCCCCGTTGCATCGCCACGAGGCGGGCTGGGCGGAGAGGAGGGAAG GGTGTATGACCTCATTTGCCGCCAGTTCCTGGCCTCAGTGTCACCAGACG CGAAATTCAAAGCCACCACAGTGGCCCTCAGCGTGGGCAAGGAAAAAGCT ACACTCTCAGCCAGAAAACTCATCTCCCCAGGGTTCCTAGCCATCCTGAA CAGATCCATGCACGTCGTGGAGGAGGAGGAAGGGGGAGGAGGAGAGGACC CAACGAGAGAAGAGGGACACTGGGTGGGAAGCGTGCACGAAGGAATGGTC CTTCCCATCGCCAGC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig76930:4..618+

>mRNA_M-pyrifera_M_contig76930.18132.1 ID=mRNA_M-pyrifera_M_contig76930.18132.1|Name=mRNA_M-pyrifera_M_contig76930.18132.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1230bp|location=Sequence derived from alignment at M-pyrifera_M_contig76930:4..618+ (Macrocystis pyrifera P11B4 male)
GAGAAGGAAGTTGGGGTGGTGAGGCCTTTTCCTCTGAATACCGTGGCGCT
GCTGAAACTGGCCTCCAAAGTGCTGGGGATGGGACCACAACGAACCATGC
AAGTGGCGGAACGGCTCTATTTGTCTGGCCTGGTCTCCTACCCCCGCACA
GAGTCCACAGCTTACCCTTCCTCCTTCGACCTCAGACACGCCATCTCCCA
GCACACCTCCCACCCGGCTTACGGCGCCTATGCCGCCTCCCTCCTCTCCT
CTGCCCCCACAAAACCGCGAAAAGGTCATGATGCAGGAGATCACCCCCCC
ATCACCCCCGTTGCATCGCCACGAGGCGGGCTGGGCGGAGAGGAGGGAAG
GGTGTATGACCTCATTTGCCGCCAGTTCCTGGCCTCAGTGTCACCAGACG
CGAAATTCAAAGCCACCACAGTGGCCCTCAGCGTGGGCAAGGAAAAAGCT
ACACTCTCAGCCAGAAAACTCATCTCCCCAGGGTTCCTAGCCATCCTGAA
CAGATCCATGCACGTCGTGGAGGAGGAGGAAGGGGGAGGAGGAGAGGACC
CAACGAGAGAAGAGGGACACTGGGTGGGAAGCGTGCACGAAGGAATGGTC
CTTCCCATCGCCAGCGAGAAGGAAGTTGGGGTGGTGAGGCCTTTTCCTCT
GAATACCGTGGCGCTGCTGAAACTGGCCTCCAAAGTGCTGGGGATGGGAC
CACAACGAACCATGCAAGTGGCGGAACGGCTCTATTTGTCTGGCCTGGTC
TCCTACCCCCGCACAGAGTCCACAGCTTACCCTTCCTCCTTCGACCTCAG
ACACGCCATCTCCCAGCACACCTCCCACCCGGCTTACGGCGCCTATGCCG
CCTCCCTCCTCTCCTCTGCCCCCACAAAACCGCGAAAAGGTCATGATGCA
GGAGATCACCCCCCCATCACCCCCGTTGCATCGCCACGAGGCGGGCTGGG
CGGAGAGGAGGGAAGGGTGTATGACCTCATTTGCCGCCAGTTCCTGGCCT
CAGTGTCACCAGACGCGAAATTCAAAGCCACCACAGTGGCCCTCAGCGTG
GGCAAGGAAAAAGCTACACTCTCAGCCAGAAAACTCATCTCCCCAGGGTT
CCTAGCCATCCTGAACAGATCCATGCACGTCGTGGAGGAGGAGGAAGGGG
GAGGAGGAGAGGACCCAACGAGAGAAGAGGGACACTGGGTGGGAAGCGTG
CACGAAGGAATGGTCCTTCCCATCGCCAGC
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