mRNA_M-pyrifera_M_contig74137.17558.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig74137.17558.1
Unique NamemRNA_M-pyrifera_M_contig74137.17558.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig74137.17558.1 vs. uniprot
Match: A0A813C0H4_9DINO (Efhb protein n=5 Tax=Sar TaxID=2698737 RepID=A0A813C0H4_9DINO)

HSP 1 Score: 214 bits (544), Expect = 3.500e-64
Identity = 111/205 (54.15%), Postives = 143/205 (69.76%), Query Frame = 1
Query:   19 RIVPLRVEAHRRATGDPLGKSRPLGHAAYARAPPAVFGKPPRADDDWGAAECLKGDARPEDMMPDEDLGRSTRPRFRNIAVDPDRVFGKPSLRTDVPPRKHTSVMSRENFGQDPEMKQLLGPSAYASRGVDHADFVASRDDTELREIFETIGYEF-SDHEFQQIAKRARQ-LDLT-PESLSVEEFRRALNEYHDHIDSTGTPPEW 624
            +++P RV A R  + D +GK R LG AAY RAPP  FG+PP + D WGAAECL+GD   E+MMPD DLG++TRP FRN   DPDRVFG PSLRTDVPPR   S+   +NFGQD  +K L+ PS + +RGVDH+DF   R+  ELRE+F++IGY + S   F  I +RA +  D+T P S+S EEF+RAL +Y + ++ TG  PEW
Sbjct:  222 KVIPRRVAAARMFSTDQVGKPRALGLAAYKRAPPDSFGRPPASGDTWGAAECLRGDFTEEEMMPDADLGKATRPGFRNTTTDPDRVFGIPSLRTDVPPRNF-SIAEPQNFGQDAPLKSLVNPSRFMTRGVDHSDFAKKREVAELRELFDSIGYSYLSAGAFAAIYQRAAERYDVTEPGSVSAEEFKRALADYLEVVEDTGEEPEW 425          
BLAST of mRNA_M-pyrifera_M_contig74137.17558.1 vs. uniprot
Match: A0A7S1CGF7_9STRA (Hypothetical protein n=1 Tax=Bicosoecida sp. CB-2014 TaxID=1486930 RepID=A0A7S1CGF7_9STRA)

HSP 1 Score: 159 bits (403), Expect = 7.720e-43
Identity = 92/219 (42.01%), Postives = 120/219 (54.79%), Query Frame = 1
Query:   13 RTRIVPLRVEAHRRATGDPLGKSRPLGHAAYARA---------PPAVFGKPPRADDDWGAAECLKGDARPEDMMPDEDLGRSTRPRFRNIAVDPDRVFGKPSLRTDVPPRKHTSVMSRENFGQDPEMKQLLGPSAYASRGVDHADFVASRDDTELREIFETIGYEFSDHEFQQIAKRAR-QLDLTPESL-SVEEFRRALNEYHDHIDSTGTPPEWFEPE 636
            R  +VP RV     A  D LGK+  LG     +              +G      D+WGA +C++GD    D +PD DLGRS    +RN + DP+RVFG P++R DVP     SV   +N+G D   + LL PS +A  GVDH DFV +R   E+R+IFE IG    DHEF Q+ +RA    DL  + + S+EEFR ALNEY D  +  G  P+WFE E
Sbjct:  278 RVVVVPKRVADAAAARADRLGKTHSLGVPKKTKIGADGKEEVDEDVTYGAAAAGADEWGAGDCIRGDYSIGDQLPDRDLGRSVAKGYRNFSHDPERVFGVPTVRADVPKPAKRSVSDNQNYGDDTSAQSLLQPSRFAVVGVDHKDFVETRGPEEIRDIFEKIGVRLDDHEFAQVWRRAATHYDLNEDGIVSIEEFRLALNEYDDAREE-GRIPDWFEEE 495          
BLAST of mRNA_M-pyrifera_M_contig74137.17558.1 vs. uniprot
Match: A0A813AZJ2_9DINO (Calmodulin n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A813AZJ2_9DINO)

HSP 1 Score: 125 bits (314), Expect = 3.680e-32
Identity = 75/164 (45.73%), Postives = 96/164 (58.54%), Query Frame = 1
Query:  142 RADDDWGAAECLKGDARPEDMMPDEDLGRSTRPRFRNIAVDPDRVFGKPSLRTDVPPRKHTSVMSRENFGQDPEMKQLLGPSAYASRGVDHADFVASRDDTELREIFETIGYEFSDHEFQQIAKRARQ-LDLTPESL-SVEEFRRALNEYHDHIDSTGTPPEWF 627
            +  D++ A +C+ G    E+ +PD+DLGRS RP FRN   DP+RVFG PS+R D+P  KHT   S            LL  S++A  GVD  DFV  R+  E+R+IFE IGY   D EF+ I +RA    DL    + S+EEFR ALNEY D  D  G  P+WF
Sbjct:   73 KRSDEFTAEDCIVGGYSIEEQLPDQDLGRSVRPGFRNFTKDPNRVFGLPSIRRDIP-SKHTVWFSM----------LLLNGSSFAELGVDDNDFVEPRNQREIRDIFERIGYSLDDGEFRAIWRRAADHYDLNGNGVVSIEEFRLALNEYLDAQDE-GKTPDWF 224          
BLAST of mRNA_M-pyrifera_M_contig74137.17558.1 vs. uniprot
Match: A0A7S2GJ40_9EUKA (Hypothetical protein n=1 Tax=Haptolina brevifila TaxID=156173 RepID=A0A7S2GJ40_9EUKA)

HSP 1 Score: 127 bits (318), Expect = 4.700e-31
Identity = 73/186 (39.25%), Postives = 103/186 (55.38%), Query Frame = 1
Query:   22 IVPLRVEAHRRATGDPLGKSRPLGHAAYARAPPAVFGKPPRADDDWGAAECLKGDARPEDMMPDEDLGRSTRPRFRNIAVDPDRVFGKPSLRTDVPPRKHTSVMSRENFGQDPEMKQLLGPSAYASRGVDHADFVASRDDTELREIFETIGYEFSDHEFQQIAKRARQLDLTPESLSVEEFRRALN 579
            +V  R+E  R  T + LG ++ LG       P  VFG P +A  +WG  EC+ G+  P +  PD DLG+S RP +RN+A   DRVFG PS+R+D+   +  SV   +N+G +     LL P  +A  GV  ADF+A+    EL +IF + G++ SD        RA+ LD     +SV+ FR ALN
Sbjct:  234 VVEKRLEDFRELTCEGLGTAKNLGFGVRPAGPEKVFGMPSQAQPEWGVRECI-GNYPPHEQQPDTDLGKSIRPGWRNMAPQ-DRVFGVPSIRSDISAPQLKSVADHQNYGDESGAGTLLYPPRFADGGVTQADFLAATSKMELGDIFRSAGFDLSDEALNDTYNRAQALDANG-LVSVQSFRMALN 416          
BLAST of mRNA_M-pyrifera_M_contig74137.17558.1 vs. uniprot
Match: A0A7S4F6I3_CHRCT (Hypothetical protein n=1 Tax=Chrysotila carterae TaxID=13221 RepID=A0A7S4F6I3_CHRCT)

HSP 1 Score: 119 bits (299), Expect = 3.560e-28
Identity = 71/186 (38.17%), Postives = 99/186 (53.23%), Query Frame = 1
Query:   22 IVPLRVEAHRRATGDPLGKSRPLGHAAYARAPPAVFGKPPRADDDWGAAECLKGDARPEDMMPDEDLGRSTRPRFRNIAVDPDRVFGKPSLRTDVPPRKHTSVMSRENFGQDPEMKQLLGPSAYASRGVDHADFVASRDDTELREIFETIGYEFSDHEFQQIAKRARQLDLTPESLSVEEFRRALN 579
            +VP R+E  R   G+PLGK + LGHA +  +   VFG P +   +WG  EC+   +  E+  PD DLG S RP +RN A D DRVFG P++R+D+      SV   +N+G +     LL PS +   G+   DF+ +R   E+ EIF   G    +     I  +A  LD     +S+E FRR LN
Sbjct:  265 VVPKRLEEFREVNGEPLGKPKYLGHAKHTPSD-HVFGTPSQRGPEWGVRECMANYSA-EEQQPDPDLGISVRPGWRNSA-DADRVFGTPTIRSDIRAPGLKSVADHQNYGDEVPAGALLYPSRFIDSGISGKDFLEARSKEEIAEIFTAAGENIDEDHIAAIFSQAATLDPKGR-VSIESFRRTLN 446          
BLAST of mRNA_M-pyrifera_M_contig74137.17558.1 vs. uniprot
Match: A0A8J8T242_HALGN (Uncharacterized protein n=1 Tax=Halteria grandinella TaxID=5974 RepID=A0A8J8T242_HALGN)

HSP 1 Score: 119 bits (298), Expect = 4.450e-28
Identity = 72/211 (34.12%), Postives = 110/211 (52.13%), Query Frame = 1
Query:   13 RTRIVPLRVEAHRRATGDPLGKSRPLGHAAYARAPPAVFG---KPPRADDDWGAAECLKGDARPEDMMPDEDLGRSTRPRFRNIAVDPD---RVFGKPSLRTDVPPRKHTSVMSRENFGQDPEMKQLLGPSAYASRGVDHADFVASRDDTELREIFETIGYEFSDHEFQQIAKRARQLD---------LTPESLSVEEFRRALNEYHDHID 600
            +T IV   VE  +    D LG+ R LG           FG        ++ W AA CL+G+   +++ PD DLG+  RP  RNI   P+   R FG P++RTD+P ++  SV    N+G +PE   LL PS +   G+   DF+ +R  TE+R +FE IG+ + + +F  +  RA+++          +  E +SV  F +A+NE H HI+
Sbjct:  230 KTVIVKKTVEDVKATQLDMLGQPRNLGQGPIPVPKDFAFGIKNTSALGENSWNAAMCLQGEPTEKELQPDRDLGKCVRPGSRNIVRRPEDANRAFGCPTIRTDIPFKEKRSVADYNNYGDEPEAVDLLFPSTFTEMGISEYDFMVTRTRTEIRSLFERIGFAYKNGKFNTMYNRAKEVQDQMRGHSVVMDYEKVSVRAFMQAINEMH-HIE 439          
BLAST of mRNA_M-pyrifera_M_contig74137.17558.1 vs. uniprot
Match: J9IY11_9SPIT (Uncharacterized protein n=1 Tax=Oxytricha trifallax TaxID=1172189 RepID=J9IY11_9SPIT)

HSP 1 Score: 118 bits (296), Expect = 7.340e-28
Identity = 67/206 (32.52%), Postives = 110/206 (53.40%), Query Frame = 1
Query:    4 GVARTRIVPLRVEAHRRATGDPLGKSRPLGHAAYARAPPAVFG-KPPRADDDWGAAECLKGDARPEDMMPDEDLGRSTRPRFRNIAVDPD---RVFGKPSLRTDVPPRKHTSVMSRENFGQDPEMKQLLGPSAYASRGVDHADFVASRDDTELREIFETIGYEFSDHEFQQIAKRARQL---DLTPESLSVEEFRRALNEYHDHID 600
            G  +T IV   VE  +  + D LGK++ LG          VFG +   ++D W AA+C+ G     ++ PD DLG+S +P  RN    P+   R+FG P++RTD+P ++  S+    N+G +PE   LL PS +   G+   DF   R   E++ +FE IG+ +   +F  +  +A++        +++SV  F +++NE H HI+
Sbjct:  222 GFPKTVIVKKTVEDQKAVSLDQLGKAKNLGQGVNQETLNQVFGVRNLTSNDQWNAAKCIHGQPNETELQPDNDLGKSNKPGCRNTVRKPEDETRIFGAPTIRTDIPFKEKRSIADYNNYGDEPEAVDLLFPSTFTEMGISEYDFQMPRARDEIKMLFERIGFTYKIGKFNAMYNKAKETMNNQAAGDNVSVRAFMQSINEMH-HIE 426          
BLAST of mRNA_M-pyrifera_M_contig74137.17558.1 vs. uniprot
Match: A0A2R5GZ70_9STRA (EF-hand domain-containing family member B n=1 Tax=Hondaea fermentalgiana TaxID=2315210 RepID=A0A2R5GZ70_9STRA)

HSP 1 Score: 116 bits (291), Expect = 5.420e-27
Identity = 76/220 (34.55%), Postives = 117/220 (53.18%), Query Frame = 1
Query:   10 ARTRIVPLRVEAHRRATGDPLGKSRPLGH---AAYARAPPAVFGKPPRADDDWGAAECLKGDARPEDMMPDEDLGRSTRPRFRNIAVDPDRVFGKPSLRTDVPPRKHTSVMSRENFGQDPEMKQLLGPSAYASRGVDHADFVASRDDTELREIFETIGYEFSDHEFQQIAKRARQL----DLTPES------LSVEEFRRALNEYHDHIDSTGTPPEWFE 630
            A+T I  + V  HR  T D LGK +  G+   A+  +       +    ++   A +C++G    E  +PDE LGR+TRP +RN     DR FG P++RTD+P     SV   +N+G D +  +LL PS + + GVD +D+V   D  ++R++   IGYE++D EF++I +RA  L    DL   S      +S++ F    NE    +D  GT P W++
Sbjct:  240 AQTEITNVAVANHRAITSDALGKPKYRGNVDSASIRKLEERQTMRRLNPEEVVTAQDCIQGSYTLEQQLPDEGLGRATRPGWRNDTSS-DRRFGAPTVRTDIPAPTVRSVSDAQNYGDDTDAAKLLYPSRFVAEGVDDSDYVDPIDREDIRDVMARIGYEYTDDEFERIFRRAASLAGVSDLDVASSVNQPIVSIQGFLSVANERFRALD-LGTEPAWWD 457          
BLAST of mRNA_M-pyrifera_M_contig74137.17558.1 vs. uniprot
Match: J9HWL1_9SPIT (Uncharacterized protein n=1 Tax=Oxytricha trifallax TaxID=1172189 RepID=J9HWL1_9SPIT)

HSP 1 Score: 116 bits (290), Expect = 5.630e-27
Identity = 74/207 (35.75%), Postives = 108/207 (52.17%), Query Frame = 1
Query:    4 GVARTRIVPLRVEAHRRATGDPLGKSRPLGHAAYARAPPAVFG-KPPRADDDWGAAECLKGDARPEDMMPDEDLGRSTRPRFRNIAVDPD---RVFGKPSLRTDVPPRKHTSVMSRENFGQDPEMKQLLGPSAYASRGVDHADFVASRDDTELREIFETIGYEFSDHEFQQIAKRARQL----DLTPESLSVEEFRRALNEYHDHID 600
            G  +T IV   VE  +  + D LGK + LG           FG +   ++D W AA+CL GD     + PD DLG++TRP  RN    P+   R+FG P++RTD+P R+  SV    N+G +PE   +L PS +   G+   DF   R   E+R +FE IG+++   +F  +  RA+Q      +    +SV  F  A+NE + HID
Sbjct:  227 GFPKTVIVKKTVEDMKAVSLDQLGKPKNLGQGLPPVPEDFRFGVRNITSNDTWNAAKCLHGDPSWNQLQPDSDLGKNTRPGTRNQVRKPEDSNRIFGCPTIRTDIPMREKRSVADYTNYGDEPEAIDVLFPSTFTEMGITEYDFQVPRPVEEIRILFERIGFKYKIGKFNAMFNRAKQFMPYSAIPSNEVSVRAFMMAVNEMN-HID 432          
BLAST of mRNA_M-pyrifera_M_contig74137.17558.1 vs. uniprot
Match: A0A078ASL9_STYLE (Uncharacterized protein n=1 Tax=Stylonychia lemnae TaxID=5949 RepID=A0A078ASL9_STYLE)

HSP 1 Score: 115 bits (287), Expect = 1.500e-26
Identity = 71/203 (34.98%), Postives = 105/203 (51.72%), Query Frame = 1
Query:    4 GVARTRIVPLRVEAHRRATGDPLGKSRPLGHAAYARAPPAVFG-KPPRADDDWGAAECLKGDARPEDMMPDEDLGRSTRPRFRNIAVDPD---RVFGKPSLRTDVPPRKHTSVMSRENFGQDPEMKQLLGPSAYASRGVDHADFVASRDDTELREIFETIGYEFSDHEFQQIAKRARQL---DLTPESLSVEEFRRALNEYHD 591
            G  +T IV   VE  +  + D LGK R LG          VFG +  + +D W AA+CL G+     + PD DLG+ T+   RN    P+   RVFG P++RTD+P R+  SV    N+G +PE   LL P  +   G+   DF   R   ++R +FE IG+ +   +F  +  RA+Q    ++  + +SV  F  A+NE H+
Sbjct:  228 GFPKTVIVKKTVEDMKAVSQDQLGKPRNLGQGRPPIPQDFVFGIRNLQNNDTWNAAKCLHGEQNYRQLQPDADLGKCTKLGTRNQVRKPEDTNRVFGCPTIRTDIPTREKRSVADYTNYGDEPEAIDLLFPQTFTEMGITEYDFQLPRGKEDIRVLFERIGFSYKVGKFNAMYNRAKQYMPYEVPSDYVSVRAFMMAVNEMHE 430          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig74137.17558.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A813C0H4_9DINO3.500e-6454.15Efhb protein n=5 Tax=Sar TaxID=2698737 RepID=A0A81... [more]
A0A7S1CGF7_9STRA7.720e-4342.01Hypothetical protein n=1 Tax=Bicosoecida sp. CB-20... [more]
A0A813AZJ2_9DINO3.680e-3245.73Calmodulin n=1 Tax=Symbiodinium sp. KB8 TaxID=2309... [more]
A0A7S2GJ40_9EUKA4.700e-3139.25Hypothetical protein n=1 Tax=Haptolina brevifila T... [more]
A0A7S4F6I3_CHRCT3.560e-2838.17Hypothetical protein n=1 Tax=Chrysotila carterae T... [more]
A0A8J8T242_HALGN4.450e-2834.12Uncharacterized protein n=1 Tax=Halteria grandinel... [more]
J9IY11_9SPIT7.340e-2832.52Uncharacterized protein n=1 Tax=Oxytricha trifalla... [more]
A0A2R5GZ70_9STRA5.420e-2734.55EF-hand domain-containing family member B n=1 Tax=... [more]
J9HWL1_9SPIT5.630e-2735.75Uncharacterized protein n=1 Tax=Oxytricha trifalla... [more]
A0A078ASL9_STYLE1.500e-2634.98Uncharacterized protein n=1 Tax=Stylonychia lemnae... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig74137contigM-pyrifera_M_contig74137:62..703 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score119.0
Seed ortholog evalue2.7e-24
Seed eggNOG ortholog5932.XP_004030855.1
Preferred nameEFHB
Hectar predicted targeting categoryother localisation
EggNOG OGs2CJU2@1,2QV2M@2759,3ZBGX@5878
Best tax levelCiliophora
Best eggNOG OGNA|NA|NA
Exons1
Model size642
Cds size642
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816639.3961246-CDS-M-pyrifera_M_contig74137:61..7031622816639.3961246-CDS-M-pyrifera_M_contig74137:61..703Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig74137 62..703 +
1692278092.6122904-CDS-M-pyrifera_M_contig74137:61..7031692278092.6122904-CDS-M-pyrifera_M_contig74137:61..703Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig74137 62..703 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig74137.17558.1prot_M-pyrifera_M_contig74137.17558.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig74137 62..703 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig74137.17558.1

>prot_M-pyrifera_M_contig74137.17558.1 ID=prot_M-pyrifera_M_contig74137.17558.1|Name=mRNA_M-pyrifera_M_contig74137.17558.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=214bp
AGVARTRIVPLRVEAHRRATGDPLGKSRPLGHAAYARAPPAVFGKPPRAD
DDWGAAECLKGDARPEDMMPDEDLGRSTRPRFRNIAVDPDRVFGKPSLRT
DVPPRKHTSVMSRENFGQDPEMKQLLGPSAYASRGVDHADFVASRDDTEL
REIFETIGYEFSDHEFQQIAKRARQLDLTPESLSVEEFRRALNEYHDHID
STGTPPEWFEPEE*
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mRNA from alignment at M-pyrifera_M_contig74137:62..703+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig74137.17558.1 ID=mRNA_M-pyrifera_M_contig74137.17558.1|Name=mRNA_M-pyrifera_M_contig74137.17558.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=642bp|location=Sequence derived from alignment at M-pyrifera_M_contig74137:62..703+ (Macrocystis pyrifera P11B4 male)
GCAGGAGTGGCAAGGACTCGCATCGTCCCGTTGCGAGTGGAGGCTCATCG CCGTGCGACTGGAGATCCGTTGGGAAAATCGAGACCTTTGGGACATGCGG CCTATGCTCGTGCTCCACCGGCCGTATTTGGGAAACCGCCTCGTGCAGAT GACGACTGGGGCGCTGCAGAGTGTCTGAAGGGAGATGCGAGGCCAGAGGA CATGATGCCGGACGAAGACTTGGGTCGGTCCACCAGGCCAAGGTTCCGAA ATATAGCCGTTGACCCCGACCGCGTCTTCGGGAAGCCTTCGCTCCGCACC GACGTGCCGCCCAGGAAACACACCTCGGTGATGAGCCGCGAAAACTTTGG ACAAGACCCCGAAATGAAGCAGCTGCTGGGTCCCTCTGCCTATGCCTCGA GGGGAGTCGACCACGCCGACTTTGTCGCCTCGAGAGACGACACGGAGCTC AGGGAAATCTTCGAGACCATCGGGTACGAGTTCTCAGACCACGAGTTCCA GCAAATCGCGAAACGCGCAAGGCAACTCGACCTCACGCCAGAGTCACTCT CCGTCGAAGAGTTCAGAAGGGCTCTCAACGAATACCACGACCACATCGAC TCCACAGGAACCCCGCCAGAGTGGTTCGAACCAGAAGAGTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig74137:62..703+

>mRNA_M-pyrifera_M_contig74137.17558.1 ID=mRNA_M-pyrifera_M_contig74137.17558.1|Name=mRNA_M-pyrifera_M_contig74137.17558.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1284bp|location=Sequence derived from alignment at M-pyrifera_M_contig74137:62..703+ (Macrocystis pyrifera P11B4 male)
GCAGGAGTGGCAAGGACTCGCATCGTCCCGTTGCGAGTGGAGGCTCATCG
CCGTGCGACTGGAGATCCGTTGGGAAAATCGAGACCTTTGGGACATGCGG
CCTATGCTCGTGCTCCACCGGCCGTATTTGGGAAACCGCCTCGTGCAGAT
GACGACTGGGGCGCTGCAGAGTGTCTGAAGGGAGATGCGAGGCCAGAGGA
CATGATGCCGGACGAAGACTTGGGTCGGTCCACCAGGCCAAGGTTCCGAA
ATATAGCCGTTGACCCCGACCGCGTCTTCGGGAAGCCTTCGCTCCGCACC
GACGTGCCGCCCAGGAAACACACCTCGGTGATGAGCCGCGAAAACTTTGG
ACAAGACCCCGAAATGAAGCAGCTGCTGGGTCCCTCTGCCTATGCCTCGA
GGGGAGTCGACCACGCCGACTTTGTCGCCTCGAGAGACGACACGGAGCTC
AGGGAAATCTTCGAGACCATCGGGTACGAGTTCTCAGACCACGAGTTCCA
GCAAATCGCGAAACGCGCAAGGCAACTCGACCTCACGCCAGAGTCACTCT
CCGTCGAAGAGTTCAGAAGGGCTCTCAACGAATACCACGACCACATCGAC
TCCACAGGAACCCCGCCAGAGTGGTTCGAACCAGAAGAGTGAGCAGGAGT
GGCAAGGACTCGCATCGTCCCGTTGCGAGTGGAGGCTCATCGCCGTGCGA
CTGGAGATCCGTTGGGAAAATCGAGACCTTTGGGACATGCGGCCTATGCT
CGTGCTCCACCGGCCGTATTTGGGAAACCGCCTCGTGCAGATGACGACTG
GGGCGCTGCAGAGTGTCTGAAGGGAGATGCGAGGCCAGAGGACATGATGC
CGGACGAAGACTTGGGTCGGTCCACCAGGCCAAGGTTCCGAAATATAGCC
GTTGACCCCGACCGCGTCTTCGGGAAGCCTTCGCTCCGCACCGACGTGCC
GCCCAGGAAACACACCTCGGTGATGAGCCGCGAAAACTTTGGACAAGACC
CCGAAATGAAGCAGCTGCTGGGTCCCTCTGCCTATGCCTCGAGGGGAGTC
GACCACGCCGACTTTGTCGCCTCGAGAGACGACACGGAGCTCAGGGAAAT
CTTCGAGACCATCGGGTACGAGTTCTCAGACCACGAGTTCCAGCAAATCG
CGAAACGCGCAAGGCAACTCGACCTCACGCCAGAGTCACTCTCCGTCGAA
GAGTTCAGAAGGGCTCTCAACGAATACCACGACCACATCGACTCCACAGG
AACCCCGCCAGAGTGGTTCGAACCAGAAGAGTGA
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