mRNA_M-pyrifera_M_contig74132.17555.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig74132.17555.1
Unique NamemRNA_M-pyrifera_M_contig74132.17555.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig74132.17555.1 vs. uniprot
Match: A0A2G9RDF4_LITCT (Sulfatase domain-containing protein (Fragment) n=1 Tax=Lithobates catesbeianus TaxID=8400 RepID=A0A2G9RDF4_LITCT)

HSP 1 Score: 98.6 bits (244), Expect = 6.020e-21
Identity = 64/189 (33.86%), Postives = 88/189 (46.56%), Query Frame = 1
Query:    1 DGRVLTDWDGPA-RMPNLRAFAREGVTFLNAYSAAPECVPSRSAFWSGRAVSQTKTWNNGMGLPLSYPISMEVLHAQSGYDGWFNGRRDFNYLGDSYQPSWAGEVPILHHTLTA-----NLSSWLRAVPGLNIPNEDKQDYLVVPSNTTRFHVLDWTRSDAGKEWLQDRAAKNNTNPFFLSQGYICPHP 549
            DGR+ +  D     +PNL      G  FLNAY+ AP C PSRSA WSG     T++WNN   L   YP  M+++ A+      F G++D+     S   S +G     H +L+A      + +W R VP L          L       R    DW   D   +W++ + AKN++ PFFL  G   PHP
Sbjct:    1 DGRLTSTLDSQVVSLPNLNFMKHHGAVFLNAYTNAPICCPSRSAMWSGLFPHLTQSWNNFKCLDPGYPTWMDLIKAKGHITQKF-GKQDYT----SGSHSLSGLEDSTHLSLSAFAFSNRVEAWTRDVPFLLRQEGRPMANLTTNRTQIRIREEDWKNVDLAVDWIR-KQAKNSSEPFFLYLGLNLPHP 183          
BLAST of mRNA_M-pyrifera_M_contig74132.17555.1 vs. uniprot
Match: UPI001AADBF0C (arylsulfatase K n=1 Tax=Rana temporaria TaxID=8407 RepID=UPI001AADBF0C)

HSP 1 Score: 97.8 bits (242), Expect = 1.960e-20
Identity = 60/184 (32.61%), Postives = 85/184 (46.20%), Query Frame = 1
Query:    1 DGRVLTDWDGPA-RMPNLRAFAREGVTFLNAYSAAPECVPSRSAFWSGRAVSQTKTWNNGMGLPLSYPISMEVLHAQSGYDGWFNGRRDFNYLGDSYQPSWAGEVPILHHTLTANLSSWLRAVPGLNIPNEDKQDYLVVPSNTTRFHVLDWTRSDAGKEWLQDRAAKNNTNPFFLSQGYICPHP 549
            DGR+ +  D     +PNL      G  FLNAY+ AP C PSRSA WSG     T++WNN   L   YP  M+++  +      F G++D+           +G      H+L+  + +W R VP L          L       R +  DW   D   +W++ + AKN++ PFFL  G   PHP
Sbjct:   46 DGRLTSTLDSQVVSLPNLNFMKHHGAVFLNAYTNAPICCPSRSAMWSGLFPHLTQSWNNFKCLDPGYPTWMDLIKEKGHITQKF-GKQDYT----------SGS-----HSLSNRVEAWTRDVPFLLRQEGRPMANLTTNKTQIRINEKDWINVDLAADWIR-KQAKNSSEPFFLYLGLNLPHP 212          
BLAST of mRNA_M-pyrifera_M_contig74132.17555.1 vs. uniprot
Match: UPI000854219E (arylsulfatase K isoform X1 n=3 Tax=Nanorana parkeri TaxID=125878 RepID=UPI000854219E)

HSP 1 Score: 96.3 bits (238), Expect = 7.000e-20
Identity = 63/187 (33.69%), Postives = 95/187 (50.80%), Query Frame = 1
Query:    1 DGRVLTDWDGP--ARMPNLRAFAREGVTFLNAYSAAPECVPSRSAFWSGRAVSQTKTWNNGMGLPLSYPISMEVLHAQSGYDGWFNGRRDFNYLGDSYQPSWAGEVPILHHTLTANLSSWLRAVPGLNIPNEDKQDYLVVPSNTTRFHVL--DWTRSDAGKEWLQDRAAKNNTNPFFLSQGYICPHP 549
            DGR LT+  G     +PNL      G  FLNAY+ AP C PSRSA WSG     T++WNN   L   YP  M+++ A+      F G++D+           +G      H+L+  + +W R VP L   +++ +    + +N T+  ++  DW  +D    W++ + AKN++ PFFL  G   PHP
Sbjct:   46 DGR-LTNTLGSHLVSLPNLNFMKHHGAMFLNAYTNAPICCPSRSAMWSGLFPHLTQSWNNFKCLDPGYPTWMDLIKAKGHITQKF-GKQDYT----------SGS-----HSLSNRVEAWTRDVPFLL--SQEGRPIANLTTNRTQIRIMEDDWKNTDLAVNWIR-KQAKNSSQPFFLYLGLNLPHP 212          
BLAST of mRNA_M-pyrifera_M_contig74132.17555.1 vs. uniprot
Match: UPI002019DEA7 (arylsulfatase K isoform X2 n=2 Tax=Perognathus longimembris pacificus TaxID=214514 RepID=UPI002019DEA7)

HSP 1 Score: 90.9 bits (224), Expect = 5.310e-18
Identity = 57/171 (33.33%), Postives = 84/171 (49.12%), Query Frame = 1
Query:   40 MPNLRAFAREGVTFLNAYSAAPECVPSRSAFWSGRAVSQTKTWNNGMGLPLSYPISMEVLHAQSGYDGWFNGRRDFNYLGDSYQPSWAGEVPILHHTLTANLSSWLRAVPGLNIPNEDKQDYLVVPSNT-TRFHVLDWTRSDAGKEWLQDRAAKNNTNPFFLSQGYICPHP 549
            +P +    ++G +FLNAY+ AP C PSR+A WSG     T++WNN  GL  +Y   M+V+    GY     G+ D+           +G     HH+++  + +W R V  L +  E +    ++P+ T  R    DW   D    WL+  A KN+T PF L  G   PHP
Sbjct:   61 LPFIDYLKKQGTSFLNAYTNAPICCPSRAAMWSGLFTHLTESWNNFKGLDENYITWMDVMEKH-GYQTQKFGKIDYT----------SG-----HHSISNRVEAWTRDVSFL-LRQEGRPMVNLIPNKTEVRVMEKDWKHIDEAVHWLRTEA-KNSTKPFVLYLGLNLPHP 213          
BLAST of mRNA_M-pyrifera_M_contig74132.17555.1 vs. uniprot
Match: A0A6I9LME3_PERMB (arylsulfatase K isoform X1 n=7 Tax=Neotominae TaxID=337963 RepID=A0A6I9LME3_PERMB)

HSP 1 Score: 89.4 bits (220), Expect = 1.840e-17
Identity = 62/186 (33.33%), Postives = 88/186 (47.31%), Query Frame = 1
Query:    1 DGRVLTDWDGP--ARMPNLRAFAREGVTFLNAYSAAPECVPSRSAFWSGRAVSQTKTWNNGMGLPLSYPISMEVLHAQSGYDGWFNGRRDFNYLGDSYQPSWAGEVPILHHTLTANLSSWLRAVPGLNIPNEDKQDYLVVPS-NTTRFHVLDWTRSDAGKEWLQDRAAKNNTNPFFLSQGYICPHP 549
            DGR LT   G    ++P +      G TFLNAY+ AP C PSR+A WSG     T++WNN  GL  +Y   M+V+    GY     G+ D+           +G     HH+++  + +W R V  L +  E +    ++P  N  R    DW  +D   +WL+  A  N + PF L  G   PHP
Sbjct:   43 DGR-LTFQPGSQVVKLPFINFMKAHGTTFLNAYTNAPICCPSRAAMWSGLFTHLTESWNNFKGLDPNYTTWMDVMEKH-GYQTQKFGKLDYT----------SG-----HHSVSNRVEAWTRDVAFL-LRQEGRPVVNLIPDKNRRRVMERDWQNTDRAIDWLRQEAI-NYSRPFVLYLGLNLPHP 209          
BLAST of mRNA_M-pyrifera_M_contig74132.17555.1 vs. uniprot
Match: A0A836UMK9_9BACT (Sulfatase domain-containing protein n=1 Tax=Candidatus Handelsmanbacteria bacterium TaxID=2099671 RepID=A0A836UMK9_9BACT)

HSP 1 Score: 69.3 bits (168), Expect = 1.610e-10
Identity = 54/178 (30.34%), Postives = 78/178 (43.82%), Query Frame = 1
Query:   25 DGPARMPNLRAFAREGVTFLNAYSAAPECVPSRSAFWSGRAVSQTKTWNNGMGLPLSYPISMEVLHAQSGYDGWFNGRRDF----NYLGDSYQPSWAGEVPILHHTLTANLSSWLRAVPGLNIPNEDKQDYLVVPSNTTRFHVLDWTRSDAGKEWLQDRAAKNNTNPFFLSQGYICPH 546
            D   R P+L   A EG+TF NAY+AAP CVP+R AF +GR  S  + W N   L  + P     + A  GY+    GR  F       G  Y+P   GE    +          L+++PG     + ++         T     D   + A   +L+++A KN   PF    G++ PH
Sbjct:   52 DPLVRTPHLDRLASEGMTFTNAYTAAPLCVPARMAFMTGRRPSANQVWTNDHILSSALPTWAHAIGAL-GYETALIGRMHFVGADQRHGFEYRP--LGEYGANYPGAPKKGGPILKSIPGTL--GQTRECVETAGYGRTTNQDFDDRVARAVCAYLEEKAGKNE-RPFAAVAGFVLPH 223          
BLAST of mRNA_M-pyrifera_M_contig74132.17555.1 vs. uniprot
Match: A0A495Z1K5_9BACT (Choline-sulfatase n=1 Tax=Candidatus Poribacteria bacterium TaxID=2026781 RepID=A0A495Z1K5_9BACT)

HSP 1 Score: 66.6 bits (161), Expect = 1.340e-9
Identity = 49/170 (28.82%), Postives = 76/170 (44.71%), Query Frame = 1
Query:   37 RMPNLRAFAREGVTFLNAYSAAPECVPSRSAFWSGRAVSQTKTWNNGMGLPLSYPISMEVLHAQSGYDGWFNGRRDFNYLGDSYQPSWAGEVPILHHTLTANLSSWLRAVPGLNIPNEDKQDYLVVPSNTTRFHVLDWTRSDAGKEWLQDRAAKNNTNPFFLSQGYICPH 546
            + P++  FA++GVTF NAY  +P C+PSR +F +GR +     W+N   L     ++   L    GYD   +G++ F  + D      A     LH      L+ W    P  + P +        P  T    V D   + A K +L+D A +    P+ L+  +I PH
Sbjct:   31 KTPHIDRFAKDGVTFTNAYCNSPLCMPSRMSFMTGRYIHHIGAWDNAAPLRPD-AVTWAHLLRDVGYDVVLSGKQHFGGM-DQLHGFRAQLARDLHAERQHGLTDWDNGTPPASRPWQGLAQ--AGPGTTQEIEVDDLAEAAAIK-YLRDPARQEQ--PWALNVSFIAPH 193          
BLAST of mRNA_M-pyrifera_M_contig74132.17555.1 vs. uniprot
Match: A0A5C6AE83_9BACT (Choline-sulfatase n=2 Tax=Rhodopirellula pilleata TaxID=2714738 RepID=A0A5C6AE83_9BACT)

HSP 1 Score: 66.2 bits (160), Expect = 1.830e-9
Identity = 58/188 (30.85%), Postives = 85/188 (45.21%), Query Frame = 1
Query:   10 VLTDWD---GPARMPNLRAFAREGVTFLNAYSAAPECVPSRSAFWSGRAVSQTKTWNNGMGLPLSYPISMEV------LHAQSGYDGWFNGRRDFNYLGDSYQPSWAGEVPILHHTLTANLSSWLRAVPGLNIPNEDKQDYLVVPSNTTRFHVLDWTRSDAGKEWLQDRAAKNNTNPFFLSQGYICPH 546
            VL D D   G    PNLR  A  GV F  AY+AAP C PSR+AF+SG A  ++  +NN   +  S P++ +       L  +SGYD +  G+    +  + +   W  +V   H    A   + L  + G       +QD+   P + T   + D   +D     L+    K +  PFFL+ G   PH
Sbjct:   41 VLEDADRYAGKVIAPNLRRLAGSGVNFTRAYTAAPVCSPSRTAFFSGVAPWKSGIYNNAQTINKSEPLNQDAVLSLAGLFKKSGYDTFGYGKITHGWDQNEH---WVEKVG--HKRDPAPPGAPLAKLSG------GEQDW--GPIHLTEEQMNDTRGADKAVAVLE----KQHDKPFFLAYGSFNPH 211          
BLAST of mRNA_M-pyrifera_M_contig74132.17555.1 vs. uniprot
Match: A0A7W7YQ96_9BACT (Arylsulfatase A-like enzyme n=1 Tax=Prosthecobacter dejongeii TaxID=48465 RepID=A0A7W7YQ96_9BACT)

HSP 1 Score: 65.9 bits (159), Expect = 2.530e-9
Identity = 58/220 (26.36%), Postives = 79/220 (35.91%), Query Frame = 1
Query:   13 LTDWDG------PARMPNLRAFAREGVTFLNAYSAAPECVPSRSAFWSGRAVSQTKT---WNNGMGLPLSYPISMEVLHAQSGYDGWFNGRRDFN----YLGDSY--------------------QPSWAGEVPILHHTLTANLSSWLRAVPGLNIPNEDKQDYLVVPSNTTRFHVLDWTRSDAGKE---------WLQDRAAKNNTNPFFLSQGYICPH 546
            L DW G       AR PN+   A  GV F NA+ AAP C+ SR+A +SGR   QT+    W    G P +  + M +  + SGY+    G+   +    +  D Y                    QPS     P   H +         A+P   +P+E         +        DW   D   E         W   +  +    PFFL  GY  PH
Sbjct:   45 LNDWIGCMKGHPQARTPNMDRLAERGVLFTNAHCAAPVCLASRTALFSGRYPDQTRVFSNWGKTKGKPPAKSLQMPLHFSGSGYETLGTGKLYHSASPEFFDDYYDTENRWSPFTQEQARYTDEEQPSKGSANP--RHVIKGGPGGHDWALPLNGLPSERN-------ATGKEGESFDWGPVDVNDEEMGDTRVTNWAMTKLEEPRAKPFFLGVGYYRPH 255          
BLAST of mRNA_M-pyrifera_M_contig74132.17555.1 vs. uniprot
Match: A0A4R7RZ42_9BACT (Iduronate 2-sulfatase n=1 Tax=Prosthecobacter fusiformis TaxID=48464 RepID=A0A4R7RZ42_9BACT)

HSP 1 Score: 64.3 bits (155), Expect = 8.520e-9
Identity = 57/211 (27.01%), Postives = 77/211 (36.49%), Query Frame = 1
Query:   13 LTDWDG------PARMPNLRAFAREGVTFLNAYSAAPECVPSRSAFWSGRAVSQT---KTWNNGMGLPLSYPISMEVLHAQSGYDGWFNGRRDFN----YLGDSYQPS--WAGEVPILHHTLTANLSSWLRAVPGLNIPNEDKQDYLVVPSN---------TTRFHVLDWTRSDAGKE---------WLQDRAAKNNTNPFFLSQGYICPH 546
            L DW G       AR PN+   A  G+ F NA+ AAP C+ SR+A +SGR   QT     W    G   +    M +  A SGY+    G+   +    +  D Y     W+         + A L +   A P   I N       ++P N         +      DW   D   E         W   +  +    PFFL  GY  PH
Sbjct:   37 LNDWIGCMKGHPQARTPNIDRLAERGMLFTNAHCAAPVCLASRTALFSGRYPDQTGVFSNWGKTKGKAPAKSWQMPLHFAASGYETLGAGKLYHSASPQFFDDYYDTENRWSPFTQEQARYIDAELPTKSSAKPRHLIQNGPGGRDWILPLNGLPSERNPDSNEGESFDWGPVDVADEEMGDTRVTQWATKKIEEKRAKPFFLGVGYYRPH 247          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig74132.17555.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A2G9RDF4_LITCT6.020e-2133.86Sulfatase domain-containing protein (Fragment) n=1... [more]
UPI001AADBF0C1.960e-2032.61arylsulfatase K n=1 Tax=Rana temporaria TaxID=8407... [more]
UPI000854219E7.000e-2033.69arylsulfatase K isoform X1 n=3 Tax=Nanorana parker... [more]
UPI002019DEA75.310e-1833.33arylsulfatase K isoform X2 n=2 Tax=Perognathus lon... [more]
A0A6I9LME3_PERMB1.840e-1733.33arylsulfatase K isoform X1 n=7 Tax=Neotominae TaxI... [more]
A0A836UMK9_9BACT1.610e-1030.34Sulfatase domain-containing protein n=1 Tax=Candid... [more]
A0A495Z1K5_9BACT1.340e-928.82Choline-sulfatase n=1 Tax=Candidatus Poribacteria ... [more]
A0A5C6AE83_9BACT1.830e-930.85Choline-sulfatase n=2 Tax=Rhodopirellula pilleata ... [more]
A0A7W7YQ96_9BACT2.530e-926.36Arylsulfatase A-like enzyme n=1 Tax=Prosthecobacte... [more]
A0A4R7RZ42_9BACT8.520e-927.01Iduronate 2-sulfatase n=1 Tax=Prosthecobacter fusi... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig74132contigM-pyrifera_M_contig74132:397..1037 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeMetazoa
Seed ortholog score101.7
Seed ortholog evalue3.8e-19
Seed eggNOG ortholog10224.XP_002732869.2
Preferred nameARSK
KEGG koko:K12376
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0004065,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005783,GO:0005788,GO:0008484,GO:0012505,GO:0016787,GO:0016788,GO:0031974,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0044422,GO:0044424,GO:0044432,GO:0044444,GO:0044446,GO:0044464,GO:0070013
EggNOG free text desc.sulfuric ester hydrolase activity
EggNOG OGs39S62@33154,3BDCF@33208,3CWGU@33213,COG3119@1,KOG3731@2759,KOG3867@2759
COG Functional cat.G
Best tax levelBilateria
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000
Exons2
Model size549
Cds size549
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816639.2559512-CDS-M-pyrifera_M_contig74132:396..5101622816639.2559512-CDS-M-pyrifera_M_contig74132:396..510Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig74132 397..510 +
1692278092.5468106-CDS-M-pyrifera_M_contig74132:396..5101692278092.5468106-CDS-M-pyrifera_M_contig74132:396..510Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig74132 397..510 +
1622816639.2676764-CDS-M-pyrifera_M_contig74132:602..10371622816639.2676764-CDS-M-pyrifera_M_contig74132:602..1037Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig74132 603..1037 +
1692278092.5573337-CDS-M-pyrifera_M_contig74132:602..10371692278092.5573337-CDS-M-pyrifera_M_contig74132:602..1037Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig74132 603..1037 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig74132.17555.1prot_M-pyrifera_M_contig74132.17555.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig74132 397..1037 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig74132.17555.1

>prot_M-pyrifera_M_contig74132.17555.1 ID=prot_M-pyrifera_M_contig74132.17555.1|Name=mRNA_M-pyrifera_M_contig74132.17555.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=183bp
DGRVLTDWDGPARMPNLRAFAREGVTFLNAYSAAPECVPSRSAFWSGRAV
SQTKTWNNGMGLPLSYPISMEVLHAQSGYDGWFNGRRDFNYLGDSYQPSW
AGEVPILHHTLTANLSSWLRAVPGLNIPNEDKQDYLVVPSNTTRFHVLDW
TRSDAGKEWLQDRAAKNNTNPFFLSQGYICPHP
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mRNA from alignment at M-pyrifera_M_contig74132:397..1037+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig74132.17555.1 ID=mRNA_M-pyrifera_M_contig74132.17555.1|Name=mRNA_M-pyrifera_M_contig74132.17555.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=641bp|location=Sequence derived from alignment at M-pyrifera_M_contig74132:397..1037+ (Macrocystis pyrifera P11B4 male)
GATGGACGAGTGCTGACGGATTGGGATGGCCCAGCAAGAATGCCAAACTT GCGGGCGTTTGCTCGTGAAGGGGTAACGTTTTTGAACGCATACTCTGCGG CTCCTGAATGTGTGGTGCGTGTTGGATTGGGTAGAGTGGGAAGGAGGGGG TGATGGGGTGATGTGGAGTGAGAAAGAGGTTGGTTTGCTTTTCTGGTGAT GCGCAGCCATCGAGATCTGCATTCTGGAGTGGTCGTGCGGTATCTCAGAC CAAGACTTGGAACAATGGGATGGGATTGCCTTTGTCATACCCGATTTCGA TGGAAGTGCTGCACGCACAGAGTGGATATGATGGTTGGTTCAATGGACGA CGCGATTTCAACTACCTGGGTGATTCATACCAGCCATCTTGGGCAGGAGA AGTACCAATTCTACATCACACACTCACAGCAAACCTTTCTTCATGGCTTC GAGCAGTGCCTGGGTTGAACATCCCGAACGAAGACAAACAAGACTATTTG GTCGTTCCTTCCAACACAACTCGATTCCATGTATTAGATTGGACCAGAAG TGATGCAGGGAAGGAATGGCTGCAAGACAGGGCAGCGAAGAACAACACAA ACCCTTTCTTCCTGAGCCAAGGTTACATTTGTCCGCACCCT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig74132:397..1037+

>mRNA_M-pyrifera_M_contig74132.17555.1 ID=mRNA_M-pyrifera_M_contig74132.17555.1|Name=mRNA_M-pyrifera_M_contig74132.17555.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1098bp|location=Sequence derived from alignment at M-pyrifera_M_contig74132:397..1037+ (Macrocystis pyrifera P11B4 male)
GATGGACGAGTGCTGACGGATTGGGATGGCCCAGCAAGAATGCCAAACTT
GCGGGCGTTTGCTCGTGAAGGGGTAACGTTTTTGAACGCATACTCTGCGG
CTCCTGAATGTGTGGATGGACGAGTGCTGACGGATTGGGATGGCCCAGCA
AGAATGCCAAACTTGCGGGCGTTTGCTCGTGAAGGGGTAACGTTTTTGAA
CGCATACTCTGCGGCTCCTGAATGTGTGCCATCGAGATCTGCATTCTGGA
GTGGTCGTGCGGTATCTCAGACCAAGACTTGGAACAATGGGATGGGATTG
CCTTTGTCATACCCGATTTCGATGGAAGTGCTGCACGCACAGAGTGGATA
TGATGGTTGGTTCAATGGACGACGCGATTTCAACTACCTGGGTGATTCAT
ACCAGCCATCTTGGGCAGGAGAAGTACCAATTCTACATCACACACTCACA
GCAAACCTTTCTTCATGGCTTCGAGCAGTGCCTGGGTTGAACATCCCGAA
CGAAGACAAACAAGACTATTTGGTCGTTCCTTCCAACACAACTCGATTCC
ATGTATTAGATTGGACCAGAAGTGATGCAGGGAAGGAATGGCTGCAAGAC
AGGGCAGCGAAGAACAACACAAACCCTTTCTTCCTGAGCCAAGGTTACAT
TTGTCCGCACCCTCCATCGAGATCTGCATTCTGGAGTGGTCGTGCGGTAT
CTCAGACCAAGACTTGGAACAATGGGATGGGATTGCCTTTGTCATACCCG
ATTTCGATGGAAGTGCTGCACGCACAGAGTGGATATGATGGTTGGTTCAA
TGGACGACGCGATTTCAACTACCTGGGTGATTCATACCAGCCATCTTGGG
CAGGAGAAGTACCAATTCTACATCACACACTCACAGCAAACCTTTCTTCA
TGGCTTCGAGCAGTGCCTGGGTTGAACATCCCGAACGAAGACAAACAAGA
CTATTTGGTCGTTCCTTCCAACACAACTCGATTCCATGTATTAGATTGGA
CCAGAAGTGATGCAGGGAAGGAATGGCTGCAAGACAGGGCAGCGAAGAAC
AACACAAACCCTTTCTTCCTGAGCCAAGGTTACATTTGTCCGCACCCT
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