mRNA_M-pyrifera_M_contig70861.16913.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig70861.16913.1
Unique NamemRNA_M-pyrifera_M_contig70861.16913.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig70861.16913.1 vs. uniprot
Match: D7FY72_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FY72_ECTSI)

HSP 1 Score: 136 bits (343), Expect = 3.200e-35
Identity = 73/107 (68.22%), Postives = 83/107 (77.57%), Query Frame = 1 
Query:    1 QITKTSAFIADGNFQRVALQFPDPLLREAPEVVWTVQARVRQLQK------EMGGGHEGGEGEGQRE-GRRKEPLIFVTGDTSYGSCCVDEVSAQHLKADAIVHYGK 300
            +I +T+AFIADGNF+RVALQFPD LLREAPEV+WT+QAR+R+L           G  E GEG+G  E      PLIFVTGDTSYGSCCVDEVSAQHLKADAIVHYG+
Sbjct:   53 EIPRTTAFIADGNFRRVALQFPDVLLREAPEVLWTLQARLRKLAAGGVRSVSQVGREEEGEGKGPSEVATTAPPLIFVTGDTSYGSCCVDEVSAQHLKADAIVHYGR 159
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BLAST of mRNA_M-pyrifera_M_contig70861.16913.1 vs. uniprot
Match: A0A6P6GAF6_ZIZJJ (2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 n=2 Tax=Ziziphus jujuba TaxID=326968 RepID=A0A6P6GAF6_ZIZJJ)

HSP 1 Score: 89.4 bits (220), Expect = 1.140e-18
Identity = 43/99 (43.43%), Postives = 63/99 (63.64%), Query Frame = 1 
Query:    1 QITKTSAFIADGNFQRVALQFPDPLLREAPEVVWTVQARVRQLQKEMGGGHEGGEGEGQREGRRKEPLIFVTGDTSYGSCCVDEVSAQHLKADAIVHYG 297
            +I +T+ FI+ G F RVALQFPD LL+++  VV  ++ ++R+L++           +  R G  K+  +FV  DT+YGSCCVDE  A H+ AD +VHYG
Sbjct:    8 EIARTAKFISGGGFTRVALQFPDELLKDSVRVVSVLRDKLRRLRESQS--------DSDRNGDVKDVSLFVMADTAYGSCCVDEAGASHINADCVVHYG 98
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BLAST of mRNA_M-pyrifera_M_contig70861.16913.1 vs. uniprot
Match: A0A803QHF4_CANSA (Reverse transcriptase Ty1/copia-type domain-containing protein n=1 Tax=Cannabis sativa TaxID=3483 RepID=A0A803QHF4_CANSA)

HSP 1 Score: 87.8 bits (216), Expect = 4.510e-18
Identity = 45/99 (45.45%), Postives = 63/99 (63.64%), Query Frame = 1 
Query:    1 QITKTSAFIADGNFQRVALQFPDPLLREAPEVVWTVQARVRQLQKEMGGGHEGGEGEGQREGRRKEPLIFVTGDTSYGSCCVDEVSAQHLKADAIVHYG 297
            +I++T  FI  GNF +VALQFPD LL+++  VV +++ ++R L           E E +R    K+  +FV  DT+YGSCCVDEV A H+ AD +VHYG
Sbjct:    8 EISRTVDFIRSGNFTKVALQFPDELLKDSVRVVKSLKDKLRWLS----------ESETERNNDFKDVSMFVMADTAYGSCCVDEVGASHIGADCVVHYG 96
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BLAST of mRNA_M-pyrifera_M_contig70861.16913.1 vs. uniprot
Match: A0A836CDL8_9STRA (Putative diphthamide biosynthesis protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CDL8_9STRA)

HSP 1 Score: 87.4 bits (215), Expect = 4.590e-18
Identity = 45/100 (45.00%), Postives = 57/100 (57.00%), Query Frame = 1 
Query:    1 QITKTSAFIADGNFQRVALQFPDPLLREAPEVVWTVQARVRQLQKEMGGGHEGGEGEGQREGRRKEPLIFVTGDTSYGSCCVDEVSAQHLKADAIVHYGK 300
            ++ +T+AF+ +G F RVALQFPD LL E+ E                                   PL+FV+GDTSYG+CCVDE+SAQHL ADAIVHYG+
Sbjct:   39 ELERTAAFVREGGFLRVALQFPDSLLHESAE-----------------------------------PLLFVSGDTSYGACCVDEISAQHLGADAIVHYGR 103
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BLAST of mRNA_M-pyrifera_M_contig70861.16913.1 vs. uniprot
Match: I2CRN6_NANGC (Diphthamide biosynthesis protein (Fragment) n=1 Tax=Nannochloropsis gaditana (strain CCMP526) TaxID=1093141 RepID=I2CRN6_NANGC)

HSP 1 Score: 84.3 bits (207), Expect = 5.130e-18
Identity = 48/97 (49.48%), Postives = 59/97 (60.82%), Query Frame = 1 
Query:   10 KTSAFIADGNFQRVALQFPDPLLREAPEVVWTVQARVRQLQKEMGGGHEGGEGEGQRE-GRRKEPLIFVTGDTSYGSCCVDEVSAQHLKADAIVHYG 297
            +T   I    + RVALQFPD +L++AP V   + A+ +  QK         E E Q +     EPL FV GDTSYGSCCVDEV+AQHL AD +VHYG
Sbjct:   23 RTLDTIECNGYCRVALQFPDEMLKDAPRVCQFLMAQSKIRQKR--------EKERQHDVSEHTEPLFFVIGDTSYGSCCVDEVAAQHLDADLVVHYG 111
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BLAST of mRNA_M-pyrifera_M_contig70861.16913.1 vs. uniprot
Match: A0A067L745_JATCU (2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 n=2 Tax=Jatropha curcas TaxID=180498 RepID=A0A067L745_JATCU)

HSP 1 Score: 87.0 bits (214), Expect = 7.570e-18
Identity = 42/99 (42.42%), Postives = 62/99 (62.63%), Query Frame = 1 
Query:    1 QITKTSAFIADGNFQRVALQFPDPLLREAPEVVWTVQARVRQLQKEMGGGHEGGEGEGQREGRRKEPLIFVTGDTSYGSCCVDEVSAQHLKADAIVHYG 297
            +I +T+ FI   NF RVALQFPD LL+++  VV  ++ +++ L+  +           ++ G  KE  +FV  DT+YGSCCVDEV A H+ AD ++HYG
Sbjct:   10 EINRTADFIHSRNFTRVALQFPDELLKDSVRVVSALRDKLQSLRASIA----------EKIGENKEARLFVMADTTYGSCCVDEVGASHIDADCVIHYG 98
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BLAST of mRNA_M-pyrifera_M_contig70861.16913.1 vs. uniprot
Match: UPI001B7F2EC7 (2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 isoform X2 n=1 Tax=Carcharodon carcharias TaxID=13397 RepID=UPI001B7F2EC7)

HSP 1 Score: 85.5 bits (210), Expect = 8.220e-18
Identity = 43/100 (43.00%), Postives = 58/100 (58.00%), Query Frame = 1 
Query:    1 QITKTSAFIADGNFQRVALQFPDPLLREAPEVVWTVQARVRQLQKEMGGGHEGGEGEGQREGRRKEPLIFVTGDTSYGSCCVDEVSAQHLKADAIVHYGK 300
            +I KT +FI D  F++VALQFPD LL +AP +V  ++                               +F+ GDTSYGSCCVDEV+A+HL+AD+IVHYG+
Sbjct:   36 EIEKTCSFITDRGFKKVALQFPDELLTDAPAIVSKLEKNTT-------------------------AKLFILGDTSYGSCCVDEVAAEHLEADSIVHYGR 110
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BLAST of mRNA_M-pyrifera_M_contig70861.16913.1 vs. uniprot
Match: W9RHS1_9ROSA (2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 n=1 Tax=Morus notabilis TaxID=981085 RepID=W9RHS1_9ROSA)

HSP 1 Score: 85.9 bits (211), Expect = 1.910e-17
Identity = 44/99 (44.44%), Postives = 62/99 (62.63%), Query Frame = 1 
Query:    1 QITKTSAFIADGNFQRVALQFPDPLLREAPEVVWTVQARVRQLQKEMGGGHEGGEGEGQREGRRKEPLIFVTGDTSYGSCCVDEVSAQHLKADAIVHYG 297
            +I++T+ FI  GNF +VALQFPD LL+++  V   ++  +R+L         GG      +G  K+  +FV  DT+YGSCCVDEV A H+ AD +VHYG
Sbjct:    8 EISRTAEFIRGGNFTKVALQFPDELLKDSVRVASALRNELRRL---------GG------DGNVKDASLFVMADTAYGSCCVDEVGASHVGADCVVHYG 91
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BLAST of mRNA_M-pyrifera_M_contig70861.16913.1 vs. uniprot
Match: A0A6P5SJ10_PRUAV (2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 n=9 Tax=Prunus TaxID=3754 RepID=A0A6P5SJ10_PRUAV)

HSP 1 Score: 85.9 bits (211), Expect = 1.930e-17
Identity = 45/99 (45.45%), Postives = 60/99 (60.61%), Query Frame = 1 
Query:    1 QITKTSAFIADGNFQRVALQFPDPLLREAPEVVWTVQARVRQLQKEMGGGHEGGEGEGQREGRRKEPLIFVTGDTSYGSCCVDEVSAQHLKADAIVHYG 297
            +I  T+ FI    F RVALQFPD LL++A  VV  ++A++R L+          E   ++     E  +FV  DT+YGSCCVDEV A H+ ADA+VHYG
Sbjct:    8 EIAPTAEFIQSNKFTRVALQFPDELLKDAVRVVSALRAKLRTLR----------ESNAEQNDDAPEVGLFVMADTAYGSCCVDEVGASHVDADAVVHYG 96
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BLAST of mRNA_M-pyrifera_M_contig70861.16913.1 vs. uniprot
Match: A0A1J7GNE0_LUPAN (2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 n=3 Tax=Lupinus TaxID=3869 RepID=A0A1J7GNE0_LUPAN)

HSP 1 Score: 85.9 bits (211), Expect = 1.960e-17
Identity = 45/98 (45.92%), Postives = 64/98 (65.31%), Query Frame = 1 
Query:    4 ITKTSAFIADGNFQRVALQFPDPLLREAPEVVWTVQARVRQLQKEMGGGHEGGEGEGQREGRRKEPLIFVTGDTSYGSCCVDEVSAQHLKADAIVHYG 297
            I  T+ FI + NF RVALQFPD LL+++  VV+ ++ R++ L++    G+ GGE         KE  ++V  DT+YGSCCVDEV A H+ AD ++HYG
Sbjct:    9 IAATAHFIHNHNFTRVALQFPDDLLKDSTRVVFALRKRLQSLRESDVAGN-GGE---------KEIGLYVMADTTYGSCCVDEVGASHINADCVIHYG 96
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig70861.16913.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FY72_ECTSI3.200e-3568.22Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
A0A6P6GAF6_ZIZJJ1.140e-1843.432-(3-amino-3-carboxypropyl)histidine synthase subu... [more]
A0A803QHF4_CANSA4.510e-1845.45Reverse transcriptase Ty1/copia-type domain-contai... [more]
A0A836CDL8_9STRA4.590e-1845.00Putative diphthamide biosynthesis protein n=1 Tax=... [more]
I2CRN6_NANGC5.130e-1849.48Diphthamide biosynthesis protein (Fragment) n=1 Ta... [more]
A0A067L745_JATCU7.570e-1842.422-(3-amino-3-carboxypropyl)histidine synthase subu... [more]
UPI001B7F2EC78.220e-1843.002-(3-amino-3-carboxypropyl)histidine synthase subu... [more]
W9RHS1_9ROSA1.910e-1744.442-(3-amino-3-carboxypropyl)histidine synthase subu... [more]
A0A6P5SJ10_PRUAV1.930e-1745.452-(3-amino-3-carboxypropyl)histidine synthase subu... [more]
A0A1J7GNE0_LUPAN1.960e-1745.922-(3-amino-3-carboxypropyl)histidine synthase subu... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig70861contigM-pyrifera_M_contig70861:750..1055 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score140.6
Seed ortholog evalue4.1e-31
Seed eggNOG ortholog2880.D7FY72
Preferred nameDPH2
KEGG koko:K17866
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0006417,GO:0006448,GO:0006464,GO:0006807,GO:0008150,GO:0008152,GO:0009058,GO:0009889,GO:0009987,GO:0010468,GO:0010556,GO:0010608,GO:0016740,GO:0016765,GO:0017182,GO:0017183,GO:0018193,GO:0018202,GO:0019222,GO:0019538,GO:0031323,GO:0031326,GO:0032268,GO:0034248,GO:0036211,GO:0043170,GO:0043412,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044424,GO:0044444,GO:0044464,GO:0050789,GO:0050794,GO:0051171,GO:0051246,GO:0060255,GO:0065007,GO:0071704,GO:0080090,GO:0090560,GO:1900247,GO:1901564,GO:2000112,GO:2000765
EggNOG free text desc.Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2
EggNOG OGsCOG1736@1,KOG2648@2759
COG Functional cat.J
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03012
Exons1
Model size306
Cds size306
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816602.4815147-CDS-M-pyrifera_M_contig70861:749..10551622816602.4815147-CDS-M-pyrifera_M_contig70861:749..1055Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig70861 750..1055 +
1692278071.4077156-CDS-M-pyrifera_M_contig70861:749..10551692278071.4077156-CDS-M-pyrifera_M_contig70861:749..1055Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig70861 750..1055 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig70861.16913.1prot_M-pyrifera_M_contig70861.16913.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig70861 750..1055 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig70861.16913.1

>prot_M-pyrifera_M_contig70861.16913.1 ID=prot_M-pyrifera_M_contig70861.16913.1|Name=mRNA_M-pyrifera_M_contig70861.16913.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=102bp
QITKTSAFIADGNFQRVALQFPDPLLREAPEVVWTVQARVRQLQKEMGGG
HEGGEGEGQREGRRKEPLIFVTGDTSYGSCCVDEVSAQHLKADAIVHYGK
H*
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mRNA from alignment at M-pyrifera_M_contig70861:750..1055+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig70861.16913.1 ID=mRNA_M-pyrifera_M_contig70861.16913.1|Name=mRNA_M-pyrifera_M_contig70861.16913.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=306bp|location=Sequence derived from alignment at M-pyrifera_M_contig70861:750..1055+ (Macrocystis pyrifera P11B4 male)
CAGATCACCAAGACCTCGGCGTTCATAGCCGACGGCAATTTCCAAAGGGT
GGCCTTGCAGTTCCCCGACCCGCTTCTCCGCGAGGCACCGGAGGTAGTGT
GGACTGTTCAAGCTCGGGTACGACAGCTGCAGAAGGAGATGGGTGGGGGG
CATGAAGGGGGGGAGGGGGAGGGACAGCGGGAGGGAAGAAGAAAGGAGCC
GCTGATTTTCGTCACCGGCGACACGTCGTACGGGAGCTGCTGCGTGGACG
AGGTCTCCGCGCAGCACCTCAAGGCGGACGCTATCGTCCACTACGGTAAG
CACTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig70861:750..1055+

>mRNA_M-pyrifera_M_contig70861.16913.1 ID=mRNA_M-pyrifera_M_contig70861.16913.1|Name=mRNA_M-pyrifera_M_contig70861.16913.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=612bp|location=Sequence derived from alignment at M-pyrifera_M_contig70861:750..1055+ (Macrocystis pyrifera P11B4 male)
CAGATCACCAAGACCTCGGCGTTCATAGCCGACGGCAATTTCCAAAGGGT
GGCCTTGCAGTTCCCCGACCCGCTTCTCCGCGAGGCACCGGAGGTAGTGT
GGACTGTTCAAGCTCGGGTACGACAGCTGCAGAAGGAGATGGGTGGGGGG
CATGAAGGGGGGGAGGGGGAGGGACAGCGGGAGGGAAGAAGAAAGGAGCC
GCTGATTTTCGTCACCGGCGACACGTCGTACGGGAGCTGCTGCGTGGACG
AGGTCTCCGCGCAGCACCTCAAGGCGGACGCTATCGTCCACTACGGTAAG
CACTGACAGATCACCAAGACCTCGGCGTTCATAGCCGACGGCAATTTCCA
AAGGGTGGCCTTGCAGTTCCCCGACCCGCTTCTCCGCGAGGCACCGGAGG
TAGTGTGGACTGTTCAAGCTCGGGTACGACAGCTGCAGAAGGAGATGGGT
GGGGGGCATGAAGGGGGGGAGGGGGAGGGACAGCGGGAGGGAAGAAGAAA
GGAGCCGCTGATTTTCGTCACCGGCGACACGTCGTACGGGAGCTGCTGCG
TGGACGAGGTCTCCGCGCAGCACCTCAAGGCGGACGCTATCGTCCACTAC
GGTAAGCACTGA
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