mRNA_M-pyrifera_M_contig70578.16845.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig70578.16845.1
Unique NamemRNA_M-pyrifera_M_contig70578.16845.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
[Close]
BLAST of mRNA_M-pyrifera_M_contig70578.16845.1 vs. uniprot
Match: A0A7Y2DTS3_9ACTN (Transcription elongation factor GreA n=1 Tax=Acidimicrobiales bacterium TaxID=2201156 RepID=A0A7Y2DTS3_9ACTN)

HSP 1 Score: 203 bits (516), Expect = 1.090e-64
Identity = 107/140 (76.43%), Postives = 119/140 (85.00%), Query Frame = 1 
Query:   16 QLSKEAFTRLKSEFDDLTTRGRIEIAQKIETARELGDLSENGDYHAAKEAQGKMEGRIQHLAGILEAAVIVEVDESSLSAVSAGCVVTIRYDGDDDVEKYLIGSIEERREGVTVMSPGSPLGKALLGASKGDTVSYEAPG 435
            QLSKEAF RL+ E+DDLT RGRIEIAQKIETARELGDLSENGDYHAAKE QGKMEGRI  L+ +LE A IVEVD +S   V AGCVV I+Y+GD+ VE YLIGSIEE+R+GV VMSPGSP+G+ALLGA  GDTV YEAPG
Sbjct:    6 QLSKEAFVRLEKEYDDLTNRGRIEIAQKIETARELGDLSENGDYHAAKEEQGKMEGRIAQLSVMLENAEIVEVDANS-QEVQAGCVVGIKYEGDEHVESYLIGSIEEKRDGVDVMSPGSPIGQALLGAKVGDTVGYEAPG 144
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BLAST of mRNA_M-pyrifera_M_contig70578.16845.1 vs. uniprot
Match: A0A7Y2II96_9ACTN (Transcription elongation factor GreA n=1 Tax=Acidimicrobiales bacterium TaxID=2201156 RepID=A0A7Y2II96_9ACTN)

HSP 1 Score: 201 bits (510), Expect = 8.920e-64
Identity = 101/144 (70.14%), Postives = 121/144 (84.03%), Query Frame = 1 
Query:    1 VVDQQQLSKEAFTRLKSEFDDLTTRGRIEIAQKIETARELGDLSENGDYHAAKEAQGKMEGRIQHLAGILEAAVIVEVDESSLSAVSAGCVVTIRYDGDDDVEKYLIGSIEERREGVTVMSPGSPLGKALLGASKGDTVSYEAP 432
            + D+ Q S EAF RLK+EFDDLT RGRIEIAQKIE+ARELGDLSENGDYHAAKE QGKMEGRI  LA +LE A IVE+D S+   V AGC++ IRY+GDD+VEKYL+GSIEE+R+G+  MSPGSP+G+AL+G + GDTV +EAP
Sbjct:    1 MADRHQFSAEAFKRLKNEFDDLTNRGRIEIAQKIESARELGDLSENGDYHAAKEEQGKMEGRINQLAALLEDAEIVEIDASA-QVVQAGCIIGIRYEGDDEVEKYLLGSIEEKRDGLDPMSPGSPIGRALMGHAVGDTVKFEAP 143
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BLAST of mRNA_M-pyrifera_M_contig70578.16845.1 vs. uniprot
Match: A0A538CVV5_9ACTN (Transcription elongation factor GreA n=1 Tax=Actinomycetia bacterium TaxID=1883427 RepID=A0A538CVV5_9ACTN)

HSP 1 Score: 192 bits (488), Expect = 1.930e-60
Identity = 99/140 (70.71%), Postives = 117/140 (83.57%), Query Frame = 1 
Query:   19 LSKEAFTRLKSEFDDLTTRGRIEIAQKIETARELGDLSENGDYHAAKEAQGKMEGRIQHLAGILEAAVIV-EVDESSLSAVSAGCVVTIRYDGDDDVEKYLIGSIEERREGVTVMSPGSPLGKALLGASKGDTVSYEAPG 435
            LSK AF RLK+E  DLTTRGRIEIA+KIETARELGDLSENGDYHAAK+ QGKMEGRI+HL  +LE+A IV ++D  S   V  GCVV++RY+GDDDVE+YL+GSIEERREG+ V+SPGSPLG AL+G+  GD +SY  PG
Sbjct:    7 LSKSAFDRLKAELTDLTTRGRIEIARKIETARELGDLSENGDYHAAKDEQGKMEGRIRHLGRLLESATIVADIDSES---VQPGCVVSVRYEGDDDVERYLVGSIEERREGLDVVSPGSPLGAALIGSKIGDDISYSWPG 143
[Close]
BLAST of mRNA_M-pyrifera_M_contig70578.16845.1 vs. uniprot
Match: A0A351EV46_9ACTN (Transcription elongation factor GreA n=1 Tax=Acidimicrobiaceae bacterium TaxID=2024894 RepID=A0A351EV46_9ACTN)

HSP 1 Score: 191 bits (486), Expect = 4.010e-60
Identity = 100/145 (68.97%), Postives = 118/145 (81.38%), Query Frame = 1 
Query:    1 VVDQQQLSKEAFTRLKSEFDDLTTRGRIEIAQKIETARELGDLSENGDYHAAKEAQGKMEGRIQHLAGILEAAVIVEVDESSLSAVSAGCVVTIRYDGDDDVEKYLIGSIEERREGVTVMSPGSPLGKALLGASKGDTVSYEAPG 435
            + D +QLS++A  RL++E +DLTTRGRI+IA+KIETARELGDLSENGDYHAAKE QGKMEGRI HL G LE  +IVE+ E++   V+ G VV IRY+GD D E YL+GSIEERREG+TVMS  SPLG ALLG+  G TVSYEAPG
Sbjct:    1 MADPKQLSQQAHDRLQAELEDLTTRGRIDIARKIETARELGDLSENGDYHAAKEEQGKMEGRIMHLEGTLEDCIIVEIPEAT-DTVTMGNVVKIRYEGDTDTETYLVGSIEERREGITVMSSSSPLGAALLGSKIGQTVSYEAPG 144
[Close]
BLAST of mRNA_M-pyrifera_M_contig70578.16845.1 vs. uniprot
Match: A0A2E5ZG62_9ACTN (Transcription elongation factor GreA n=3 Tax=Acidimicrobiaceae bacterium TaxID=2024894 RepID=A0A2E5ZG62_9ACTN)

HSP 1 Score: 188 bits (478), Expect = 6.830e-59
Identity = 98/141 (69.50%), Postives = 117/141 (82.98%), Query Frame = 1 
Query:   13 QQLSKEAFTRLKSEFDDLTTRGRIEIAQKIETARELGDLSENGDYHAAKEAQGKMEGRIQHLAGILEAAVIVEVDESSLSAVSAGCVVTIRYDGDDDVEKYLIGSIEERREGVTVMSPGSPLGKALLGASKGDTVSYEAPG 435
            QQLS+EA  RL +E +DLTTRGRI+IA+KIE ARELGDLSENGDYHAAKE QGKMEGRI HL  ILE A IVEV +   S V+ G VV++ YDGDD+ E+ L+GSIEE+R+ V V+SPGSPLG+ALLG+S GD+VS+EAPG
Sbjct:    5 QQLSQEAHDRLAAELEDLTTRGRIDIARKIEAARELGDLSENGDYHAAKEEQGKMEGRILHLEAILEKAEIVEVADGDPSVVATGMVVSVVYDGDDEPERMLVGSIEEQRDDVLVVSPGSPLGEALLGSSVGDSVSFEAPG 145
[Close]
BLAST of mRNA_M-pyrifera_M_contig70578.16845.1 vs. uniprot
Match: A0A7X7D4D5_9ACTN (Transcription elongation factor GreA n=1 Tax=Acidimicrobiales bacterium TaxID=2201156 RepID=A0A7X7D4D5_9ACTN)

HSP 1 Score: 187 bits (476), Expect = 1.330e-58
Identity = 97/138 (70.29%), Postives = 112/138 (81.16%), Query Frame = 1 
Query:   19 LSKEAFTRLKSEFDDLTTRGRIEIAQKIETARELGDLSENGDYHAAKEAQGKMEGRIQHLAGILEAAVIVEVDESSLSAVSAGCVVTIRYDGDDDVEKYLIGSIEERREGVTVMSPGSPLGKALLGASKGDTVSYEAP 432
            LS+ AF RLK E DDL TRGR++IA+KIETARELGDLSENGDYHAAKE QGKMEGRI HLA ++E A IVE   + +  + AG VV+IRY+GDDDVE+YL+GSIEER EG+ VMSPGSPLG AL+G   GD VSYE P
Sbjct:    7 LSQAAFDRLKEEHDDLVTRGRLDIARKIETARELGDLSENGDYHAAKEEQGKMEGRIIHLANLIENAEIVE--NAGVDEIRAGVVVSIRYEGDDDVERYLLGSIEERVEGIDVMSPGSPLGAALIGKKAGDVVSYETP 142
[Close]
BLAST of mRNA_M-pyrifera_M_contig70578.16845.1 vs. uniprot
Match: A0A538BB20_9ACTN (Transcription elongation factor GreA n=1 Tax=Actinomycetia bacterium TaxID=1883427 RepID=A0A538BB20_9ACTN)

HSP 1 Score: 185 bits (470), Expect = 1.090e-57
Identity = 92/140 (65.71%), Postives = 114/140 (81.43%), Query Frame = 1 
Query:   16 QLSKEAFTRLKSEFDDLTTRGRIEIAQKIETARELGDLSENGDYHAAKEAQGKMEGRIQHLAGILEAAVIVEVDESSLSAVSAGCVVTIRYDGDDDVEKYLIGSIEERREGVTVMSPGSPLGKALLGASKGDTVSYEAPG 435
            QLS++A+ RL++E DDLTTRGR+EIA  IE ARELGDLSENGDYHAAK+ QGKME RI+ L  +L+ A IV+   +S   V+AG +VTIRY+GDDD E YLIGSIEERR+ ++V+SPGSPLG+AL+G + GD V YEAPG
Sbjct:    5 QLSRDAYDRLQAELDDLTTRGRVEIATAIERARELGDLSENGDYHAAKDDQGKMEARIRQLQAMLKGAEIVDAPSASSGTVAAGSIVTIRYEGDDDTESYLIGSIEERRDDISVISPGSPLGQALVGRNTGDVVEYEAPG 144
[Close]
BLAST of mRNA_M-pyrifera_M_contig70578.16845.1 vs. uniprot
Match: A0A2D9PTY7_9ACTN (Transcription elongation factor GreA n=3 Tax=root TaxID=1 RepID=A0A2D9PTY7_9ACTN)

HSP 1 Score: 184 bits (468), Expect = 2.270e-57
Identity = 95/145 (65.52%), Postives = 118/145 (81.38%), Query Frame = 1 
Query:    1 VVDQQQLSKEAFTRLKSEFDDLTTRGRIEIAQKIETARELGDLSENGDYHAAKEAQGKMEGRIQHLAGILEAAVIVEVDESSLSAVSAGCVVTIRYDGDDDVEKYLIGSIEERREGVTVMSPGSPLGKALLGASKGDTVSYEAPG 435
            + + QQLS+EA  RL +EF DLTTRGRI+IA+KIETARELGDLSENGDYHAAKE QGKMEGRI  L  ILE + IVE+   ++  V+ G VV + ++GDD+ E+ L+GSIEE+R+ V V+SPGSPLG+ALLGA+ GDTVS+EAPG
Sbjct:    1 MAEAQQLSQEAHNRLVAEFQDLTTRGRIDIARKIETARELGDLSENGDYHAAKEEQGKMEGRIMQLESILENSEIVEIATGTVDLVAPGTVVAVVFEGDDEPERMLVGSIEEKRDDVGVVSPGSPLGEALLGAAVGDTVSFEAPG 145
[Close]
BLAST of mRNA_M-pyrifera_M_contig70578.16845.1 vs. uniprot
Match: A0A2E4SV22_9ACTN (Transcription elongation factor GreA n=2 Tax=Acidimicrobiaceae bacterium TaxID=2024894 RepID=A0A2E4SV22_9ACTN)

HSP 1 Score: 184 bits (468), Expect = 2.270e-57
Identity = 94/140 (67.14%), Postives = 114/140 (81.43%), Query Frame = 1 
Query:   16 QLSKEAFTRLKSEFDDLTTRGRIEIAQKIETARELGDLSENGDYHAAKEAQGKMEGRIQHLAGILEAAVIVEVDESSLSAVSAGCVVTIRYDGDDDVEKYLIGSIEERREGVTVMSPGSPLGKALLGASKGDTVSYEAPG 435
            QLS+EA  RL +EF+DLTTRGRI+IA+KIETARELGDLSENGDYHAAKE QGKMEGRI HL  IL  A IVE+       V+ G +V + Y+GDD+ E+ L+GSIEE+R+ V V+SPGSPLG+ALLG++ GDTVS+EAPG
Sbjct:    6 QLSQEAHDRLAAEFEDLTTRGRIDIARKIETARELGDLSENGDYHAAKEEQGKMEGRILHLESILNKAEIVELSAGGADVVAPGTIVAVVYEGDDEPERMLVGSIEEQRDDVLVVSPGSPLGEALLGSAVGDTVSFEAPG 145
[Close]
BLAST of mRNA_M-pyrifera_M_contig70578.16845.1 vs. uniprot
Match: UPI001C625D89 (transcription elongation factor GreA n=1 Tax=Iamia sp. SCSIO 61187 TaxID=2722752 RepID=UPI001C625D89)

HSP 1 Score: 184 bits (467), Expect = 3.120e-57
Identity = 97/142 (68.31%), Postives = 114/142 (80.28%), Query Frame = 1 
Query:    7 DQQQLSKEAFTRLKSEFDDLTTRGRIEIAQKIETARELGDLSENGDYHAAKEAQGKMEGRIQHLAGILEAAVIVEVDESSLSAVSAGCVVTIRYDGDDDVEKYLIGSIEERREGVTVMSPGSPLGKALLGASKGDTVSYEAP 432
            D  QLS+ A+ RLK+E+DDLTTRGRI+IA+KIETARELGDLSENGDYHAAKE QGKM+GRI HL  ILE   IV  D +S   V+ G +VTIRY+GDDD E+YL+GSIEER + + VMSPGSPLG+AL+G   GD VSYE P
Sbjct:    4 DTTQLSQAAYDRLKAEYDDLTTRGRIDIARKIETARELGDLSENGDYHAAKEEQGKMQGRILHLQAILENHEIV--DTASGGTVAPGSIVTIRYEGDDDTERYLLGSIEERHDDLDVMSPGSPLGEALVGHVAGDVVSYETP 143
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig70578.16845.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7Y2DTS3_9ACTN1.090e-6476.43Transcription elongation factor GreA n=1 Tax=Acidi... [more]
A0A7Y2II96_9ACTN8.920e-6470.14Transcription elongation factor GreA n=1 Tax=Acidi... [more]
A0A538CVV5_9ACTN1.930e-6070.71Transcription elongation factor GreA n=1 Tax=Actin... [more]
A0A351EV46_9ACTN4.010e-6068.97Transcription elongation factor GreA n=1 Tax=Acidi... [more]
A0A2E5ZG62_9ACTN6.830e-5969.50Transcription elongation factor GreA n=3 Tax=Acidi... [more]
A0A7X7D4D5_9ACTN1.330e-5870.29Transcription elongation factor GreA n=1 Tax=Acidi... [more]
A0A538BB20_9ACTN1.090e-5765.71Transcription elongation factor GreA n=1 Tax=Actin... [more]
A0A2D9PTY7_9ACTN2.270e-5765.52Transcription elongation factor GreA n=3 Tax=root ... [more]
A0A2E4SV22_9ACTN2.270e-5767.14Transcription elongation factor GreA n=2 Tax=Acidi... [more]
UPI001C625D893.120e-5768.31transcription elongation factor GreA n=1 Tax=Iamia... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig70578contigM-pyrifera_M_contig70578:1..435 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score160.6
Seed ortholog evalue5.5e-37
Seed eggNOG ortholog1121877.JQKF01000003_gene1451
Preferred namegreA
KEGG koko:K03624
Hectar predicted targeting categoryother localisation
GOsGO:0001098,GO:0001108,GO:0003674,GO:0005488,GO:0005515,GO:0005575,GO:0005618,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0005886,GO:0006139,GO:0006351,GO:0006354,GO:0006355,GO:0006725,GO:0006807,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009889,GO:0009987,GO:0010467,GO:0010468,GO:0010556,GO:0016020,GO:0016070,GO:0018130,GO:0019219,GO:0019222,GO:0019438,GO:0030312,GO:0031323,GO:0031326,GO:0032774,GO:0032784,GO:0034641,GO:0034645,GO:0034654,GO:0042221,GO:0043170,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044271,GO:0044424,GO:0044444,GO:0044464,GO:0046483,GO:0046677,GO:0050789,GO:0050794,GO:0050896,GO:0051171,GO:0051252,GO:0060255,GO:0065007,GO:0071704,GO:0071944,GO:0080090,GO:0090304,GO:0097659,GO:1901360,GO:1901362,GO:1901576,GO:1903506,GO:2000112,GO:2001141
EggNOG free text desc.Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides
EggNOG OGs2GNZV@201174,4CN6V@84992,COG0782@1,COG0782@2
COG Functional cat.K
Best tax levelAcidimicrobiia
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03021
Exons1
Model size435
Cds size435
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816598.5986063-CDS-M-pyrifera_M_contig70578:0..4351622816598.5986063-CDS-M-pyrifera_M_contig70578:0..435Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig70578 1..435 -
1692278069.2651188-CDS-M-pyrifera_M_contig70578:0..4351692278069.2651188-CDS-M-pyrifera_M_contig70578:0..435Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig70578 1..435 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig70578.16845.1prot_M-pyrifera_M_contig70578.16845.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig70578 1..435 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig70578.16845.1

>prot_M-pyrifera_M_contig70578.16845.1 ID=prot_M-pyrifera_M_contig70578.16845.1|Name=mRNA_M-pyrifera_M_contig70578.16845.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=145bp
VVDQQQLSKEAFTRLKSEFDDLTTRGRIEIAQKIETARELGDLSENGDYH
AAKEAQGKMEGRIQHLAGILEAAVIVEVDESSLSAVSAGCVVTIRYDGDD
DVEKYLIGSIEERREGVTVMSPGSPLGKALLGASKGDTVSYEAPG
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mRNA from alignment at M-pyrifera_M_contig70578:1..435-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig70578.16845.1 ID=mRNA_M-pyrifera_M_contig70578.16845.1|Name=mRNA_M-pyrifera_M_contig70578.16845.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=435bp|location=Sequence derived from alignment at M-pyrifera_M_contig70578:1..435- (Macrocystis pyrifera P11B4 male)
GTGGTTGACCAACAGCAGCTCTCAAAAGAAGCATTCACACGTCTGAAGAG
CGAATTCGACGACCTCACCACGCGTGGGCGGATCGAAATCGCTCAGAAGA
TCGAGACCGCACGCGAACTCGGCGACTTGTCTGAGAACGGCGACTACCAC
GCGGCAAAAGAAGCGCAGGGCAAGATGGAAGGCCGGATCCAGCATCTCGC
CGGCATCCTCGAGGCCGCCGTGATCGTCGAAGTCGACGAGTCCAGTCTCT
CTGCCGTGTCCGCCGGGTGCGTCGTGACGATTCGCTACGACGGTGACGAC
GATGTCGAGAAGTACCTGATCGGCTCGATCGAAGAACGCCGCGAGGGCGT
CACGGTGATGTCGCCGGGTTCCCCGCTCGGCAAGGCGCTCCTCGGCGCAT
CCAAGGGCGACACCGTCTCCTACGAGGCTCCCGGC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig70578:1..435-

>mRNA_M-pyrifera_M_contig70578.16845.1 ID=mRNA_M-pyrifera_M_contig70578.16845.1|Name=mRNA_M-pyrifera_M_contig70578.16845.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=870bp|location=Sequence derived from alignment at M-pyrifera_M_contig70578:1..435- (Macrocystis pyrifera P11B4 male)
GTGGTTGACCAACAGCAGCTCTCAAAAGAAGCATTCACACGTCTGAAGAG
CGAATTCGACGACCTCACCACGCGTGGGCGGATCGAAATCGCTCAGAAGA
TCGAGACCGCACGCGAACTCGGCGACTTGTCTGAGAACGGCGACTACCAC
GCGGCAAAAGAAGCGCAGGGCAAGATGGAAGGCCGGATCCAGCATCTCGC
CGGCATCCTCGAGGCCGCCGTGATCGTCGAAGTCGACGAGTCCAGTCTCT
CTGCCGTGTCCGCCGGGTGCGTCGTGACGATTCGCTACGACGGTGACGAC
GATGTCGAGAAGTACCTGATCGGCTCGATCGAAGAACGCCGCGAGGGCGT
CACGGTGATGTCGCCGGGTTCCCCGCTCGGCAAGGCGCTCCTCGGCGCAT
CCAAGGGCGACACCGTCTCCTACGAGGCTCCCGGCGTGGTTGACCAACAG
CAGCTCTCAAAAGAAGCATTCACACGTCTGAAGAGCGAATTCGACGACCT
CACCACGCGTGGGCGGATCGAAATCGCTCAGAAGATCGAGACCGCACGCG
AACTCGGCGACTTGTCTGAGAACGGCGACTACCACGCGGCAAAAGAAGCG
CAGGGCAAGATGGAAGGCCGGATCCAGCATCTCGCCGGCATCCTCGAGGC
CGCCGTGATCGTCGAAGTCGACGAGTCCAGTCTCTCTGCCGTGTCCGCCG
GGTGCGTCGTGACGATTCGCTACGACGGTGACGACGATGTCGAGAAGTAC
CTGATCGGCTCGATCGAAGAACGCCGCGAGGGCGTCACGGTGATGTCGCC
GGGTTCCCCGCTCGGCAAGGCGCTCCTCGGCGCATCCAAGGGCGACACCG
TCTCCTACGAGGCTCCCGGC
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