mRNA_M-pyrifera_M_contig70445.16818.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig70445.16818.1
Unique NamemRNA_M-pyrifera_M_contig70445.16818.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig70445.16818.1 vs. uniprot
Match: A0A5A8DCS8_CAFRO (Lactamase_B domain-containing protein n=1 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8DCS8_CAFRO)

HSP 1 Score: 161 bits (407), Expect = 7.150e-44
Identity = 91/171 (53.22%), Postives = 113/171 (66.08%), Query Frame = 1
Query:    1 HAVPCVGYSFQEPASTGSLQMAHVRPLLAANKEALAAKGIRPPERLLSRFKAGEVIPLPDGSTLDPANPLLMSPPVPGRKVVWFGDTCQPSPAAAALAKDADLLIHEATNALTVLDTAPG------ATRETVRAKTISHGHSTAEMAGAFAAATNPHLLLLTHFSGRYKAG 495
            H+VPCVG+ F EP   G L +  VRP+L  NKEALAA G+R PE LL   K G+ + LPDG+ LDPA+P +++P   GR++  FGDTC PS AA   +  AD+L+HEATNALT  D   G      A    VRAK +SHGHST +M GAFA A +  LL+L+HFS RYK G
Sbjct:  218 HSVPCVGWVFTEPPKPGRLCIEAVRPVLRRNKEALAASGVRVPESLLRDLKRGDRVSLPDGTVLDPADPAVIAPASQGRRLAVFGDTCAPSLAALRESFGADVLVHEATNALTPADGPVGDAASQRAAYGAVRAKAVSHGHSTPQMGGAFARAADAGLLVLSHFSARYKGG 388          
BLAST of mRNA_M-pyrifera_M_contig70445.16818.1 vs. uniprot
Match: A0A5A8EEB2_CAFRO (Lactamase_B domain-containing protein n=3 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8EEB2_CAFRO)

HSP 1 Score: 161 bits (407), Expect = 5.050e-43
Identity = 91/171 (53.22%), Postives = 113/171 (66.08%), Query Frame = 1
Query:    1 HAVPCVGYSFQEPASTGSLQMAHVRPLLAANKEALAAKGIRPPERLLSRFKAGEVIPLPDGSTLDPANPLLMSPPVPGRKVVWFGDTCQPSPAAAALAKDADLLIHEATNALTVLDTAPG------ATRETVRAKTISHGHSTAEMAGAFAAATNPHLLLLTHFSGRYKAG 495
            H+VPCVG+ F EP   G L +  VRP+L  NKEALAA G+R PE LL   K G+ + LPDG+ LDPA+P +++P   GR++  FGDTC PS AA   +  AD+L+HEATNALT  D   G      A    VRAK +SHGHST +M GAFA A +  LL+L+HFS RYK G
Sbjct:  382 HSVPCVGWVFTEPPKPGRLCIEAVRPVLRRNKEALAASGVRVPESLLRDLKRGDRVSLPDGTVLDPADPAVIAPASQGRRLAVFGDTCAPSLAALRESFGADVLVHEATNALTPADGPVGDAASQRAAYGAVRAKAVSHGHSTPQMGGAFARAADAGLLVLSHFSARYKGG 552          
BLAST of mRNA_M-pyrifera_M_contig70445.16818.1 vs. uniprot
Match: A0A0L0HDL4_SPIPD (Ribonuclease Z n=2 Tax=Spizellomyces TaxID=4815 RepID=A0A0L0HDL4_SPIPD)

HSP 1 Score: 138 bits (348), Expect = 4.360e-36
Identity = 85/184 (46.20%), Postives = 113/184 (61.41%), Query Frame = 1
Query:    1 HAVPCVGYSFQEPASTGSLQMAHVRPLLAANKEALAAKGIRPPERLLSRFKAGEVIPLPDGSTLDPANPLL---MSPPVPGRKVVWFGDTCQPSPAAAALAKDADLLIHEATNALTVLDTAPGATRETVRAKTISHGHSTAEMAGAFAAATNPHLLLLTHFSGRYKAGLN--GLGVMLQVAAQA 537
            H VPC+GY  QEP   G++ ++ ++P L  N++ALA +GI+ P  L+ +  + + + LPDG+ L     LL   ++PP PGRK+   GDT  PSP    LA D+DLLIHEATNA    D A G T  +V+  TISHGHST EMAGAFA       L+L HFS RYK  L+   L +M ++ A A
Sbjct:  190 HTVPCIGYVIQEPPHPGNINISTLQPHLERNRKALAEQGIKNPSILIGKLMSQQSLTLPDGTNL-----LLKEHLTPPTPGRKITILGDTNDPSP-IIHLAIDSDLLIHEATNACLKADLASGQTPSSVQESTISHGHSTPEMAGAFARLVGARKLVLNHFSSRYKGDLSEESLQIMEEIRALA 367          
BLAST of mRNA_M-pyrifera_M_contig70445.16818.1 vs. uniprot
Match: A0A1X2GKB9_9FUNG (Lactamase_B domain-containing protein n=1 Tax=Hesseltinella vesiculosa TaxID=101127 RepID=A0A1X2GKB9_9FUNG)

HSP 1 Score: 133 bits (335), Expect = 1.670e-34
Identity = 77/164 (46.95%), Postives = 105/164 (64.02%), Query Frame = 1
Query:    1 HAVPCVGYSFQEPASTGSLQMAHVRPLLAANKEALAAKGIRPPERLLSRF-KAGEVIPLPDGSTLDPANPLLMSPPVPGRKVVWFGDTCQPSPAAAALAKDADLLIHEATNALTVLDTAPGATRETVRAKTISHGHSTAEMAGAFAAATNPHLLLLTHFSGRYK 489
            H++P +G+  QEP   G++ +A +RP+L  N +AL A+GI+ P  LL +  ++G+ I LPDG+ ++P +       VPGR+VV  GDTC PS   A+      LL+HEATNALT +D A   T E V  + I+HGHST +MAGAFA     H L+LTHFS RYK
Sbjct:  157 HSIPSIGFVIQEPDILGTIDIAAIRPVLLRNADALLAQGIKQPLSLLGKLQRSGQPIDLPDGTRIEPPSK------VPGRQVVILGDTCDPSN-IASFCSQPQLLVHEATNALTSMD-AGSVTEELVEERCIAHGHSTPQMAGAFAKRLGAHTLILTHFSSRYK 312          
BLAST of mRNA_M-pyrifera_M_contig70445.16818.1 vs. uniprot
Match: G7E364_MIXOS (Lactamase_B domain-containing protein n=1 Tax=Mixia osmundae (strain CBS 9802 / IAM 14324 / JCM 22182 / KY 12970) TaxID=764103 RepID=G7E364_MIXOS)

HSP 1 Score: 130 bits (327), Expect = 5.100e-33
Identity = 83/170 (48.82%), Postives = 100/170 (58.82%), Query Frame = 1
Query:    1 HAVPCVGYSFQEPASTGSLQMAHVRPLLAANKEALAAKGIRPPERLLSRF-KAGEVIPLPDGSTLDPANPLLMSPPVPGRKVVWFGDTCQPSPAAAALAKDADLLIHEATNA-LTVLDTAPGATRETVRAKTISHGHSTAEMAGAFAAATNPHLLLLTHFSGRYKAGLNG 504
            H VPCVGY+ QE A  G   MA + P L AN +AL A+G+  P  LL    + GE I LPDG+ LDP + L      PGRK+V  GDT +PS   A LA DADLL+HEATNA L      P  T E+VR +    GHSTAE+AGA+A       L L H S RYK  ++G
Sbjct:  180 HTVPCVGYAIQERARRGKFDMAQIGPRLGANAKALKARGMSNPHTLLGVIQRTGEPITLPDGTRLDPPSDL------PGRKIVILGDTYEPSEEMAQLALDADLLVHEATNAWLPQHGAKPEDTMESVRQQARDRGHSTAEIAGAWAHKVRARELALNHISSRYKGPVDG 343          
BLAST of mRNA_M-pyrifera_M_contig70445.16818.1 vs. uniprot
Match: A0A418CMW6_9STRA (Uncharacterized protein n=1 Tax=Aphanomyces astaci TaxID=112090 RepID=A0A418CMW6_9STRA)

HSP 1 Score: 124 bits (310), Expect = 1.400e-31
Identity = 82/180 (45.56%), Postives = 108/180 (60.00%), Query Frame = 1
Query:    1 HAVPCVGYSFQEPASTGSLQMAHVRPLLAANKEALAAKGIRPPERLLSRFKAGEVIPLPDGSTLDPANPLLMSPPVPGRKVVWFGDTCQPSPAA-AALAKD--ADLLIHEATNALTVLDTAPGATRETVRAKTISHGHSTAEMAGAFAAATNPHLLLLTHFSGRYKA--GLNGLGVMLQV 525
            H VP VGY  +EPAS G L++  VRP+L  N  AL   G++ P  LL R K GE + +PDG+ + P   L ++ P PGR V+  GDT     +A A L++D   D+++HEATNA    D A G T+E+V   T +HGHST +MAGAFA A     L+L HFS RYK     + + VM QV
Sbjct:   66 HTVPTVGYLLREPASPGRLKIELVRPILQRNSAAL---GLKNPLSLLPRLKRGESLVMPDGTVMSPE--LCLASPRPGRVVLILGDTSNALESAFATLSQDDMVDVVVHEATNACLPCDVATGLTKESVEINTKAHGHSTPDMAGAFARAIKCKRLILNHFSSRYKGDGSTDSMDVMDQV 240          
BLAST of mRNA_M-pyrifera_M_contig70445.16818.1 vs. uniprot
Match: A0A165L3U8_EXIGL (Metallo-hydrolase/oxidoreductase n=1 Tax=Exidia glandulosa HHB12029 TaxID=1314781 RepID=A0A165L3U8_EXIGL)

HSP 1 Score: 126 bits (317), Expect = 1.410e-31
Identity = 79/165 (47.88%), Postives = 98/165 (59.39%), Query Frame = 1
Query:    1 HAVPCVGYSFQEPASTGSLQMAHVRPLLAANKEALAAKGIRPPERLLSRFKAGEVIPLPDGSTLDPANPLLMSPPVPGRKVVWFGDTCQPSPAAAALAKDADLLIHEATNALTV---LDTAPGATRETVRAKTISHGHSTAEMAGAFAAATNPHLLLLTHFSGRY 486
            H+VPCVGY   E    G ++ +   P +  NK AL   GI  P  LL++ +AGE + LPDG+ L+P       P  PGRKVV  GDT  P P  A+LA  AD+LIHEATNA      LDT    T ETV+A+ + HGHST +MAGAFA      +L+L HFS RY
Sbjct:  193 HSVPCVGYVVSELPIPGKIEPSLYAPHINRNKVALVKSGIAKPMSLLAKLQAGETLTLPDGAVLEPP------PRRPGRKVVILGDTYDPRP-IASLAHGADVLIHEATNAYLPGVDLDTKESDTAETVQARAVGHGHSTPQMAGAFARDVGARMLVLNHFSARY 350          
BLAST of mRNA_M-pyrifera_M_contig70445.16818.1 vs. uniprot
Match: A0A0E9N9E2_SAICN (Lactamase_B domain-containing protein n=1 Tax=Saitoella complicata (strain BCRC 22490 / CBS 7301 / JCM 7358 / NBRC 10748 / NRRL Y-17804) TaxID=698492 RepID=A0A0E9N9E2_SAICN)

HSP 1 Score: 125 bits (314), Expect = 1.140e-30
Identity = 82/166 (49.40%), Postives = 98/166 (59.04%), Query Frame = 1
Query:    1 HAVPCVGYSFQEPASTGSLQMAHVRPLLAANKEALAAKGIRPPERLLSRFKAGEVIPLPDGSTLDPANPLLMSPPVPGRKVVWFGDTCQPSPAAAALAKDADLLIHEATNA-LTVLDTAPGATR--ETVRAKTISHGHSTAEMAGAFAAATNPHLLLLTHFSGRYK 489
            H+ PC+G+  QE    G +  A   P L  NKEALAA+GI  P +LL+  + G  I LPDG+ L P  P +      GRK+   GDT  PSP  A L  DADLLIHEATNA L  +D A  +T   ETV+ +TI HGHST +MAGAFA       L L HFS RYK
Sbjct:  269 HSAPCIGFVVQETNVPGKMNPALYAPHLKRNKEALAAEGIANPMKLLATIQQGTKITLPDGTVLTPPAPRV------GRKIAILGDTHDPSP-MAVLCDDADLLIHEATNAHLPDIDAATKSTDTFETVQERTICHGHSTPQMAGAFARRIGAKRLCLNHFSSRYK 427          
BLAST of mRNA_M-pyrifera_M_contig70445.16818.1 vs. uniprot
Match: W4GQF7_9STRA (Lactamase_B domain-containing protein n=5 Tax=Aphanomyces astaci TaxID=112090 RepID=W4GQF7_9STRA)

HSP 1 Score: 122 bits (306), Expect = 7.040e-30
Identity = 82/180 (45.56%), Postives = 106/180 (58.89%), Query Frame = 1
Query:    1 HAVPCVGYSFQEPASTGSLQMAHVRPLLAANKEALAAKGIRPPERLLSRFKAGEVIPLPDGSTLDPANPLLMSPPVPGRKVVWFGDTCQPSPAA-AALAKD--ADLLIHEATNALTVLDTAPGATRETVRAKTISHGHSTAEMAGAFAAATNPHLLLLTHFSGRYKA--GLNGLGVMLQV 525
            H VP VGY  +EPAS G LQ+  VRP+L  N  AL   G++ P  LL R K GE + +PDG+ + P   L ++ P PGR V+  GDT     +A   L++D   D+++HEATNA    D A G T E+V   T +HGHST +MAGAFA A     L+L HFS RYK     + + VM QV
Sbjct:  208 HTVPTVGYLLREPASPGRLQIELVRPILQRNSAAL---GLKNPLSLLPRLKRGESLVMPDGTVMSPE--LCLASPRPGRVVLILGDTSNALESAFVTLSQDDVVDVVVHEATNACLPCDMAAGLTTESVEINTKAHGHSTPDMAGAFARAIKCKRLILNHFSSRYKGDGSTDSMDVMDQV 382          
BLAST of mRNA_M-pyrifera_M_contig70445.16818.1 vs. uniprot
Match: A0A1V9Y5K8_9STRA (Lactamase_B domain-containing protein n=1 Tax=Achlya hypogyna TaxID=1202772 RepID=A0A1V9Y5K8_9STRA)

HSP 1 Score: 120 bits (302), Expect = 2.690e-29
Identity = 77/166 (46.39%), Postives = 95/166 (57.23%), Query Frame = 1
Query:    1 HAVPCVGYSFQEPASTGSLQMAHVRPLLAANKEALAAKGIRPPERLLSRFKAGEVIPLPDGSTLDPANPLLMSPPVPGRKVVWFGDTCQP---SPAAAALAKDADLLIHEATNALTVLDTAPGATRETVRAKTISHGHSTAEMAGAFAAATNPHLLLLTHFSGRYK 489
            H VP VGY  +EPA  G L +  V P+L  NK AL   G+R P  LL + KAG  + +PDG+ L PA    + P  PGR V+  GDT      S  A  +  + DL++HEATNA    D A G T   V A+T SHGHST +MAGAFA A     L+L HFS RY+
Sbjct:  202 HTVPTVGYLLREPALPGRLNIEAVNPVLQRNKAAL---GLRNPLSLLPQLKAGATLTMPDGTVLSPAT--CVGPTKPGRVVLLLGDTSDAVATSFVALTVNDEVDLVVHEATNACLPSDVASGTTAAEVEAQTRSHGHSTPDMAGAFAKAVGARRLVLNHFSSRYR 362          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig70445.16818.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A5A8DCS8_CAFRO7.150e-4453.22Lactamase_B domain-containing protein n=1 Tax=Cafe... [more]
A0A5A8EEB2_CAFRO5.050e-4353.22Lactamase_B domain-containing protein n=3 Tax=Cafe... [more]
A0A0L0HDL4_SPIPD4.360e-3646.20Ribonuclease Z n=2 Tax=Spizellomyces TaxID=4815 Re... [more]
A0A1X2GKB9_9FUNG1.670e-3446.95Lactamase_B domain-containing protein n=1 Tax=Hess... [more]
G7E364_MIXOS5.100e-3348.82Lactamase_B domain-containing protein n=1 Tax=Mixi... [more]
A0A418CMW6_9STRA1.400e-3145.56Uncharacterized protein n=1 Tax=Aphanomyces astaci... [more]
A0A165L3U8_EXIGL1.410e-3147.88Metallo-hydrolase/oxidoreductase n=1 Tax=Exidia gl... [more]
A0A0E9N9E2_SAICN1.140e-3049.40Lactamase_B domain-containing protein n=1 Tax=Sait... [more]
W4GQF7_9STRA7.040e-3045.56Lactamase_B domain-containing protein n=5 Tax=Apha... [more]
A0A1V9Y5K8_9STRA2.690e-2946.39Lactamase_B domain-containing protein n=1 Tax=Achl... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig70445contigM-pyrifera_M_contig70445:2..538 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeFungi
Seed ortholog score143.7
Seed ortholog evalue8.5e-32
Seed eggNOG ortholog109760.SPPG_06028T0
KEGG koko:K00784
KEGG Pathwayko03013,map03013
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Metallo-hydrolase oxidoreductase
EggNOG OGs38EKA@33154,3NZP3@4751,COG1234@1,KOG2121@2759
EC3.1.26.11
COG Functional cat.H
Best tax levelFungi
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03016
Exons1
Model size537
Cds size537
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816597.098169-CDS-M-pyrifera_M_contig70445:1..5381622816597.098169-CDS-M-pyrifera_M_contig70445:1..538Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig70445 2..538 +
1692278068.3654544-CDS-M-pyrifera_M_contig70445:1..5381692278068.3654544-CDS-M-pyrifera_M_contig70445:1..538Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig70445 2..538 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig70445.16818.1prot_M-pyrifera_M_contig70445.16818.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig70445 2..538 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig70445.16818.1

>prot_M-pyrifera_M_contig70445.16818.1 ID=prot_M-pyrifera_M_contig70445.16818.1|Name=mRNA_M-pyrifera_M_contig70445.16818.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=179bp
HAVPCVGYSFQEPASTGSLQMAHVRPLLAANKEALAAKGIRPPERLLSRF
KAGEVIPLPDGSTLDPANPLLMSPPVPGRKVVWFGDTCQPSPAAAALAKD
ADLLIHEATNALTVLDTAPGATRETVRAKTISHGHSTAEMAGAFAAATNP
HLLLLTHFSGRYKAGLNGLGVMLQVAAQA
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mRNA from alignment at M-pyrifera_M_contig70445:2..538+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig70445.16818.1 ID=mRNA_M-pyrifera_M_contig70445.16818.1|Name=mRNA_M-pyrifera_M_contig70445.16818.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=537bp|location=Sequence derived from alignment at M-pyrifera_M_contig70445:2..538+ (Macrocystis pyrifera P11B4 male)
CATGCCGTGCCCTGTGTCGGGTACTCTTTCCAAGAGCCAGCGAGCACCGG CTCTTTGCAGATGGCGCATGTGCGCCCTCTGTTGGCTGCGAACAAGGAGG CTTTGGCAGCAAAAGGCATTCGACCTCCTGAGCGATTGCTGTCTCGCTTC AAGGCTGGAGAAGTGATTCCCCTTCCCGATGGATCCACTCTTGACCCTGC CAACCCTCTGCTCATGAGTCCTCCAGTGCCCGGGCGAAAAGTCGTCTGGT TCGGTGACACGTGTCAGCCATCACCGGCGGCAGCTGCGCTTGCCAAAGAC GCAGACCTTCTCATCCACGAAGCCACAAACGCACTCACTGTGCTCGACAC AGCACCAGGGGCTACCAGAGAAACAGTGAGAGCCAAAACTATCTCACACG GACACTCCACTGCAGAAATGGCAGGTGCATTCGCCGCTGCCACAAACCCA CACCTCCTCCTCCTCACCCACTTCTCCGGACGATACAAAGCCGGTCTCAA CGGCCTTGGAGTCATGCTACAGGTCGCAGCACAAGCT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig70445:2..538+

>mRNA_M-pyrifera_M_contig70445.16818.1 ID=mRNA_M-pyrifera_M_contig70445.16818.1|Name=mRNA_M-pyrifera_M_contig70445.16818.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1074bp|location=Sequence derived from alignment at M-pyrifera_M_contig70445:2..538+ (Macrocystis pyrifera P11B4 male)
CATGCCGTGCCCTGTGTCGGGTACTCTTTCCAAGAGCCAGCGAGCACCGG
CTCTTTGCAGATGGCGCATGTGCGCCCTCTGTTGGCTGCGAACAAGGAGG
CTTTGGCAGCAAAAGGCATTCGACCTCCTGAGCGATTGCTGTCTCGCTTC
AAGGCTGGAGAAGTGATTCCCCTTCCCGATGGATCCACTCTTGACCCTGC
CAACCCTCTGCTCATGAGTCCTCCAGTGCCCGGGCGAAAAGTCGTCTGGT
TCGGTGACACGTGTCAGCCATCACCGGCGGCAGCTGCGCTTGCCAAAGAC
GCAGACCTTCTCATCCACGAAGCCACAAACGCACTCACTGTGCTCGACAC
AGCACCAGGGGCTACCAGAGAAACAGTGAGAGCCAAAACTATCTCACACG
GACACTCCACTGCAGAAATGGCAGGTGCATTCGCCGCTGCCACAAACCCA
CACCTCCTCCTCCTCACCCACTTCTCCGGACGATACAAAGCCGGTCTCAA
CGGCCTTGGAGTCATGCTACAGGTCGCAGCACAAGCTCATGCCGTGCCCT
GTGTCGGGTACTCTTTCCAAGAGCCAGCGAGCACCGGCTCTTTGCAGATG
GCGCATGTGCGCCCTCTGTTGGCTGCGAACAAGGAGGCTTTGGCAGCAAA
AGGCATTCGACCTCCTGAGCGATTGCTGTCTCGCTTCAAGGCTGGAGAAG
TGATTCCCCTTCCCGATGGATCCACTCTTGACCCTGCCAACCCTCTGCTC
ATGAGTCCTCCAGTGCCCGGGCGAAAAGTCGTCTGGTTCGGTGACACGTG
TCAGCCATCACCGGCGGCAGCTGCGCTTGCCAAAGACGCAGACCTTCTCA
TCCACGAAGCCACAAACGCACTCACTGTGCTCGACACAGCACCAGGGGCT
ACCAGAGAAACAGTGAGAGCCAAAACTATCTCACACGGACACTCCACTGC
AGAAATGGCAGGTGCATTCGCCGCTGCCACAAACCCACACCTCCTCCTCC
TCACCCACTTCTCCGGACGATACAAAGCCGGTCTCAACGGCCTTGGAGTC
ATGCTACAGGTCGCAGCACAAGCT
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