mRNA_M-pyrifera_M_contig70282.16794.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig70282.16794.1
Unique NamemRNA_M-pyrifera_M_contig70282.16794.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig70282.16794.1 vs. uniprot
Match: A0A6H5KDC0_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KDC0_9PHAE)

HSP 1 Score: 111 bits (277), Expect = 2.060e-25
Identity = 66/169 (39.05%), Postives = 99/169 (58.58%), Query Frame = 1
Query:    1 LESLRLRSIPIAGTAVAPGSSYRTMRRVCAIQGKIDTEISSSCNAFLVLRDAYSDRIADVVEALQGMELKGHVLRADKVESNPKREKEAMKRTVFVGQIPWSASEDDIRAHFVERLSYGADAVESVRLLRDRATQKSRGVAHVRLSSFATAAEALTLHGTTLDGRELAV 507
            +ES+RLRS+ + GTAV      + +R+VC  +G +D E+ SS NA++V +D  S    +   A  G ++ G  +R D+ +S          R+VF+G +P  A+E+++R  F  +L  G  +VE VRL+RD+AT   +G  +V L+  A AA AL LHGT L GR L V
Sbjct:  342 VESVRLRSVAVKGTAVDKAGDQQLVRKVCVNRGMVDEEVKSSVNAYVVYKDGAS---VEKALAANGTDVGGKHVRVDRAKSGEYDHT----RSVFLGNLPMDAAEEEVRELFASKLEGGPKSVEGVRLVRDKATLVGKGFGYVLLADRALAAAALALHGTKLKGRPLRV 503          
BLAST of mRNA_M-pyrifera_M_contig70282.16794.1 vs. uniprot
Match: A0A485LPI4_9STRA (Aste57867_24108 protein n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485LPI4_9STRA)

HSP 1 Score: 105 bits (262), Expect = 1.970e-23
Identity = 70/172 (40.70%), Postives = 99/172 (57.56%), Query Frame = 1
Query:    1 LESLRLRSIPIAGTAVAPGSSYRTMRRVCAIQGKIDTEISSSCNAFLVLRDAYSDRIADVVEALQGMELKGHVLRADKVESNPKREKEAMKRTVFVGQIPWSASEDDIRAHFVERLSYGADA--VESVRLLRDRATQKSRGVAHVRLSSFATAAEALTLHGTTLDGRELAVS 510
            +ES+RLRS+P+AG  V        +++VCA + KI  +   SCNA+LV  D  S    D   AL G E    V+R D+     K      KR+VFVG + ++A++DD+R HF + L  G D   VESVRL+RD+ T   +G  +V L   +TAA+AL L+G  +  REL V+
Sbjct:  177 IESVRLRSVPVAGCKVDKNGKQNLVKKVCANK-KIFVDGRDSCNAYLVFVDHTS---VDAALALNGSEFCSKVIRVDR-----KTVSMDAKRSVFVGNVAFTATDDDVRKHFEKALRDGDDTPVVESVRLIRDKTTHLGKGFGYVLLRDVSTAAKALALNGQKMGKRELRVT 339          
BLAST of mRNA_M-pyrifera_M_contig70282.16794.1 vs. uniprot
Match: D8LFV0_ECTSI (White-brown-complex ABC transporter family (Partial) (Fragment) n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LFV0_ECTSI)

HSP 1 Score: 105 bits (262), Expect = 3.200e-23
Identity = 64/169 (37.87%), Postives = 96/169 (56.80%), Query Frame = 1
Query:    1 LESLRLRSIPIAGTAVAPGSSYRTMRRVCAIQGKIDTEISSSCNAFLVLRDAYSDRIADVVEALQGMELKGHVLRADKVESNPKREKEAMKRTVFVGQIPWSASEDDIRAHFVERLSYGADAVESVRLLRDRATQKSRGVAHVRLSSFATAAEALTLHGTTLDGRELAV 507
            +ES+RLRS+ + G AV      + +R+VC  +G +D E+ SS NA++V +D  S    +   A  G ++ G  +R D+ +S          R+VF+G +P  A E+++R  F  +L  G  +VE VRL+RD+AT   +G  +V L+  A AA AL L GT L GR L V
Sbjct:  356 VESVRLRSVAVQGMAVDKAGDQQLVRKVCVNRGMVDEEVKSSVNAYVVYKDCAS---VEKALAANGTDVGGKHVRVDRAKSGEYDHT----RSVFLGNLPMDAGEEEVRELFASKLEGGPKSVEGVRLVRDKATLVGKGFGYVLLADRALAAAALALQGTKLKGRPLRV 517          
BLAST of mRNA_M-pyrifera_M_contig70282.16794.1 vs. uniprot
Match: A0A5A8EQE5_CAFRO (RRM domain-containing protein n=2 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8EQE5_CAFRO)

HSP 1 Score: 101 bits (251), Expect = 6.470e-22
Identity = 68/172 (39.53%), Postives = 94/172 (54.65%), Query Frame = 1
Query:    1 LESLRLRSIPIAGTAVAPGSSYRTMRRVCAIQGKIDTEISSSCNAFLVLRDAYSDRIADVVEALQGMELKGHVLRADKVESNPKREKEAMKRTVFVGQIPWSASEDDIRAHFVERLSYGADAVESVRLLRDRATQKSRGVAHVRLSSFATAAEALTL--HGTTLDGRELAVS 510
            +ES+R RS+P+   A A    +RT RR   IQG+       S NA++V + A  D +A   + L    + GH LRAD             KRTVFVGQ+P+ A+E+ +RA F   L  G D ++SVR+ RDR T + +G+A+VR       AEAL L   G  L GR+L V+
Sbjct:  232 IESIRFRSVPLTNVAAAKEGGHRTQRRAAFIQGRFAG--GESMNAYVVFK-AEDDAVA-ARKVLHNAVVLGHHLRADIASGTDAAADN--KRTVFVGQLPYDATEESVRALFASNLGGGDDVIDSVRVPRDRVTNQGKGIAYVRFVDSVPVAEALALANKGLELSGRKLQVA 397          
BLAST of mRNA_M-pyrifera_M_contig70282.16794.1 vs. uniprot
Match: A0A5A8CJA2_CAFRO (RRM domain-containing protein n=1 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8CJA2_CAFRO)

HSP 1 Score: 101 bits (251), Expect = 8.080e-22
Identity = 68/172 (39.53%), Postives = 94/172 (54.65%), Query Frame = 1
Query:    1 LESLRLRSIPIAGTAVAPGSSYRTMRRVCAIQGKIDTEISSSCNAFLVLRDAYSDRIADVVEALQGMELKGHVLRADKVESNPKREKEAMKRTVFVGQIPWSASEDDIRAHFVERLSYGADAVESVRLLRDRATQKSRGVAHVRLSSFATAAEALTL--HGTTLDGRELAVS 510
            +ES+R RS+P+   A A    +RT RR   IQG+       S NA++V + A  D +A   + L    + GH LRAD             KRTVFVGQ+P+ A+E+ +RA F   L  G D ++SVR+ RDR T + +G+A+VR       AEAL L   G  L GR+L V+
Sbjct:  233 IESIRFRSVPLTNVAAAKEGGHRTQRRAAFIQGRFAG--GESMNAYVVFK-AEDDAVA-ARKVLHNAVVLGHHLRADIASGTDAAADN--KRTVFVGQLPYDATEESVRALFASNLGGGDDVIDSVRVPRDRVTNQGKGIAYVRFVDSVPVAEALALANKGLELSGRKLQVA 398          
BLAST of mRNA_M-pyrifera_M_contig70282.16794.1 vs. uniprot
Match: A0A024TR24_9STRA (Uncharacterized protein n=2 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024TR24_9STRA)

HSP 1 Score: 99.0 bits (245), Expect = 4.600e-21
Identity = 65/172 (37.79%), Postives = 98/172 (56.98%), Query Frame = 1
Query:    1 LESLRLRSIPIAGTAVAPGSSYRTMRRVCAIQGKIDTEISSSCNAFLVLRDAYSDRIADVVEALQGMELKGHVLRADKVESNPKREKEAMKRTVFVGQIPWSASEDDIRAHFVERLSYGAD--AVESVRLLRDRATQKSRGVAHVRLSSFATAAEALTLHGTTLDGRELAVS 510
            ++S+RLRS+P+AG  V        +++VC  + K+  E   SCNA++V  D  S    D    L G E     LR D+     K      KRTVFVG + ++A++DD+R HF + L   AD  AVESVR++RD++T   +G  +V     +TAA+AL+LH + +  RE+ V+
Sbjct:  207 VKSVRLRSVPVAGCKVDQNGKQNLVKKVCTNK-KLFVEGRDSCNAYVVFVDESS---VDAALQLNGSEFFKKCLRVDR-----KNVSMDAKRTVFVGNLAFTATDDDVRNHFEKALREDADTPAVESVRIIRDKSTHLGKGFGYVLFKDVSTAAKALSLHESKMGKREIRVT 369          
BLAST of mRNA_M-pyrifera_M_contig70282.16794.1 vs. uniprot
Match: A0A7R9UAS7_9STRA (Hypothetical protein (Fragment) n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9UAS7_9STRA)

HSP 1 Score: 96.3 bits (238), Expect = 1.670e-20
Identity = 57/167 (34.13%), Postives = 99/167 (59.28%), Query Frame = 1
Query:    1 LESLRLRSIPIAGTAVAPGSSYRTMRRVCAIQGKIDTEISSSCNAFLVLRDAYSDRIADVVEALQGMELKGHVLRADKVESNPKREKEAMKRTVFVGQIPWSASEDDIRAHFVERLS--YGADAVESVRLLRDRATQKSRGVAHVRLSSFATAAEALTLHGTTLDGR 495
            +E++RLRS+ + G  +    +   MR+V A +G++DT++  SCNA++   D   + +   +EA  G EL    +R D+++ +      +  RT+F+G +P++  E+D+R  F+E LS  +G D V+ VRL+RD++T   +G  ++ LSS A  A+AL L   +  GR
Sbjct:  113 VEAVRLRSMAVKGVKIDEAGNQSLMRKVAANRGELDTDVKKSCNAYVRYED--KEAVDKALEA-DGAELGDRHIRVDRLQGSQALFNAS--RTLFLGSLPYTVDEEDVRHKFIELLSASHGGDVVQGVRLIRDKSTLLGKGFGYLLLSSKAAVADALRLPEVSFGGR 274          
BLAST of mRNA_M-pyrifera_M_contig70282.16794.1 vs. uniprot
Match: A0A067CXJ9_SAPPC (Uncharacterized protein n=2 Tax=Saprolegnia TaxID=4769 RepID=A0A067CXJ9_SAPPC)

HSP 1 Score: 95.9 bits (237), Expect = 4.460e-20
Identity = 69/177 (38.98%), Postives = 101/177 (57.06%), Query Frame = 1
Query:    1 LESLRLRSIPIAGTAVAPGSSYRTMRRVCAIQGKIDTEISSSCNAFLVLRDAYSDRIADVVEALQGMELKGHVLRADK--VESNPKREKEAMKRTVFVGQIPWSASEDDIRAHFVERLSY-----GADAVESVRLLRDRATQKSRGVAHVRLSSFATAAEALTLHGTTLDGRELAVS 510
            +ES RLRS+P+AG AV        +++VCA + K+  E   +CNA++V   A  D IA  + AL G +     +R D+  + ++PKR       TVFVG + ++A+ED+IR HF   L       G+  VESVR++RD+ T   +G  +V L    TAA+AL L G+ +  REL VS
Sbjct:  148 VESARLRSVPVAGCAVDQHGKQNLVKKVCANK-KLFVEGRDNCNAYVVF--ASEDSIAAAL-ALNGADFHEKKIRVDRKVLTTDPKR-------TVFVGNMAFTATEDEIRNHFESNLEKDDEDEGSALVESVRIIRDKDTHLGKGFGYVLLKDVTTAAKALKLDGSKIGKRELRVS 313          
BLAST of mRNA_M-pyrifera_M_contig70282.16794.1 vs. uniprot
Match: A0A6G0X8S4_9STRA (Uncharacterized protein n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0X8S4_9STRA)

HSP 1 Score: 95.9 bits (237), Expect = 4.510e-20
Identity = 67/173 (38.73%), Postives = 100/173 (57.80%), Query Frame = 1
Query:    1 LESLRLRSIPIAGTAVAPGSSYRTMRRVCAIQGKIDTEISSSCNAFLVLRDAYSDRIADVVEALQGMELKGHVLRAD-KVESNPKREKEAMKRTVFVGQIPWSASEDDIRAHFVERLSYGAD--AVESVRLLRDRATQKSRGVAHVRLSSFATAAEALTLHGTTLDGRELAVS 510
            +ES+RLRS+P+AG  V        +++VCA + KI  E   SCNA+LV  D  S + A     L G       LR D KV +   R      R+VFVG + ++A++D++R HF + L   AD   VESVR++RD+ T K +G  +V     +TAA+AL+L+G+ +  RE+ V+
Sbjct:  160 IESVRLRSLPVAGCKVDQAGKQSLVKKVCANK-KIFVEGRDSCNAYLVFVDESSIQAA---LKLNGSTFFNKTLRVDRKVVTMDAR------RSVFVGNLSFTATDDEVREHFDKALRDDADEMVVESVRIIRDKTTHKGKGFGYVLFKDVSTAAKALSLNGSKMGKREIRVT 322          
BLAST of mRNA_M-pyrifera_M_contig70282.16794.1 vs. uniprot
Match: A0A7S2WDD9_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2WDD9_9STRA)

HSP 1 Score: 92.8 bits (229), Expect = 8.170e-20
Identity = 59/169 (34.91%), Postives = 98/169 (57.99%), Query Frame = 1
Query:   10 LRLRSIPIAGTAVAPGSSYRTMRRVCAIQGKIDTEISSSCNAFLVLRDAYSDRIADVVEALQGMELKGHVLRADKVESNPKREKEAMKRTVFVGQIPWSASEDDIRAHFVERLSYGAD---AVESVRLLRDRATQKSRGVAHVRLSSFATAAEALTLHGTTLDGRELAV 507
            +R+RS+PI GTAV    + R ++RVC+   K  ++  ++CNA++V R   ++   +      G+  +   LR D   S  +++ +A  R+VF+G +P+ A E+D+R HF   L   A+    VE VR++RD  TQ+ +G  +V LS+    A+AL LH +T  GR++ V
Sbjct:    1 VRIRSVPITGTAVDQPGNERLVKRVCSNTKKF-SDAKATCNAYVVFR---AEAAVEAAVQSDGLVFRERHLRID---SATRKDFDAQ-RSVFLGNLPFDADEEDVRKHFASVLETSAEDGPVVEGVRIVRDALTQRGKGFGYVLLSNRTAVAQALQLHESTFAGRKIRV 161          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig70282.16794.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KDC0_9PHAE2.060e-2539.05Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A485LPI4_9STRA1.970e-2340.70Aste57867_24108 protein n=1 Tax=Aphanomyces stella... [more]
D8LFV0_ECTSI3.200e-2337.87White-brown-complex ABC transporter family (Partia... [more]
A0A5A8EQE5_CAFRO6.470e-2239.53RRM domain-containing protein n=2 Tax=Cafeteria ro... [more]
A0A5A8CJA2_CAFRO8.080e-2239.53RRM domain-containing protein n=1 Tax=Cafeteria ro... [more]
A0A024TR24_9STRA4.600e-2137.79Uncharacterized protein n=2 Tax=Aphanomyces invada... [more]
A0A7R9UAS7_9STRA1.670e-2034.13Hypothetical protein (Fragment) n=1 Tax=Pinguiococ... [more]
A0A067CXJ9_SAPPC4.460e-2038.98Uncharacterized protein n=2 Tax=Saprolegnia TaxID=... [more]
A0A6G0X8S4_9STRA4.510e-2038.73Uncharacterized protein n=1 Tax=Aphanomyces euteic... [more]
A0A7S2WDD9_9STRA8.170e-2034.91Hypothetical protein n=1 Tax=Rhizochromulina marin... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig70282contigM-pyrifera_M_contig70282:604..1113 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score108.6
Seed ortholog evalue2.9e-21
Seed eggNOG ortholog2880.D8LFV0
Preferred namemyef2
KEGG koko:K05679,ko:K05681
KEGG TC3.A.1.204
KEGG Pathwayko01523,ko02010,ko04976,map01523,map02010,map04976
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003676,GO:0003723,GO:0003729,GO:0005488,GO:0005515,GO:0097159,GO:1901363
EggNOG free text desc.ATPase activity, coupled to transmembrane movement of substances
EggNOG OGsCOG1131@1,KOG0061@2759,KOG0118@1,KOG0118@2759
COG Functional cat.V
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko02000,ko04090,ko04147
Exons1
Model size510
Cds size510
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816595.8323216-CDS-M-pyrifera_M_contig70282:603..11131622816595.8323216-CDS-M-pyrifera_M_contig70282:603..1113Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig70282 604..1113 -
1692278067.7279303-CDS-M-pyrifera_M_contig70282:603..11131692278067.7279303-CDS-M-pyrifera_M_contig70282:603..1113Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig70282 604..1113 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig70282.16794.1prot_M-pyrifera_M_contig70282.16794.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig70282 604..1113 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig70282.16794.1

>prot_M-pyrifera_M_contig70282.16794.1 ID=prot_M-pyrifera_M_contig70282.16794.1|Name=mRNA_M-pyrifera_M_contig70282.16794.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=170bp
LESLRLRSIPIAGTAVAPGSSYRTMRRVCAIQGKIDTEISSSCNAFLVLR
DAYSDRIADVVEALQGMELKGHVLRADKVESNPKREKEAMKRTVFVGQIP
WSASEDDIRAHFVERLSYGADAVESVRLLRDRATQKSRGVAHVRLSSFAT
AAEALTLHGTTLDGRELAVS
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mRNA from alignment at M-pyrifera_M_contig70282:604..1113-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig70282.16794.1 ID=mRNA_M-pyrifera_M_contig70282.16794.1|Name=mRNA_M-pyrifera_M_contig70282.16794.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=510bp|location=Sequence derived from alignment at M-pyrifera_M_contig70282:604..1113- (Macrocystis pyrifera P11B4 male)
CTGGAATCACTGCGTTTGCGCAGTATTCCGATCGCTGGGACTGCAGTCGC ACCTGGATCAAGCTATCGCACTATGCGGCGGGTATGTGCAATCCAAGGAA AGATCGACACTGAGATTTCTTCATCTTGCAATGCATTTTTGGTGCTCCGT GATGCCTATTCTGATCGTATCGCAGATGTTGTAGAAGCTTTGCAAGGAAT GGAACTAAAGGGTCATGTATTACGTGCTGACAAGGTGGAATCAAATCCCA AGCGGGAGAAAGAAGCCATGAAGCGCACCGTCTTTGTTGGGCAGATTCCT TGGAGTGCCTCGGAAGATGATATTCGAGCCCATTTCGTCGAACGACTTTC GTATGGGGCTGATGCAGTTGAAAGTGTACGTTTGTTACGTGACCGCGCTA CCCAAAAGTCCAGGGGAGTTGCACATGTACGACTCTCTTCCTTCGCTACC GCAGCAGAAGCACTCACTCTCCATGGCACAACACTTGATGGCAGAGAACT GGCAGTGTCA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig70282:604..1113-

>mRNA_M-pyrifera_M_contig70282.16794.1 ID=mRNA_M-pyrifera_M_contig70282.16794.1|Name=mRNA_M-pyrifera_M_contig70282.16794.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1020bp|location=Sequence derived from alignment at M-pyrifera_M_contig70282:604..1113- (Macrocystis pyrifera P11B4 male)
CTGGAATCACTGCGTTTGCGCAGTATTCCGATCGCTGGGACTGCAGTCGC
ACCTGGATCAAGCTATCGCACTATGCGGCGGGTATGTGCAATCCAAGGAA
AGATCGACACTGAGATTTCTTCATCTTGCAATGCATTTTTGGTGCTCCGT
GATGCCTATTCTGATCGTATCGCAGATGTTGTAGAAGCTTTGCAAGGAAT
GGAACTAAAGGGTCATGTATTACGTGCTGACAAGGTGGAATCAAATCCCA
AGCGGGAGAAAGAAGCCATGAAGCGCACCGTCTTTGTTGGGCAGATTCCT
TGGAGTGCCTCGGAAGATGATATTCGAGCCCATTTCGTCGAACGACTTTC
GTATGGGGCTGATGCAGTTGAAAGTGTACGTTTGTTACGTGACCGCGCTA
CCCAAAAGTCCAGGGGAGTTGCACATGTACGACTCTCTTCCTTCGCTACC
GCAGCAGAAGCACTCACTCTCCATGGCACAACACTTGATGGCAGAGAACT
GGCAGTGTCACTGGAATCACTGCGTTTGCGCAGTATTCCGATCGCTGGGA
CTGCAGTCGCACCTGGATCAAGCTATCGCACTATGCGGCGGGTATGTGCA
ATCCAAGGAAAGATCGACACTGAGATTTCTTCATCTTGCAATGCATTTTT
GGTGCTCCGTGATGCCTATTCTGATCGTATCGCAGATGTTGTAGAAGCTT
TGCAAGGAATGGAACTAAAGGGTCATGTATTACGTGCTGACAAGGTGGAA
TCAAATCCCAAGCGGGAGAAAGAAGCCATGAAGCGCACCGTCTTTGTTGG
GCAGATTCCTTGGAGTGCCTCGGAAGATGATATTCGAGCCCATTTCGTCG
AACGACTTTCGTATGGGGCTGATGCAGTTGAAAGTGTACGTTTGTTACGT
GACCGCGCTACCCAAAAGTCCAGGGGAGTTGCACATGTACGACTCTCTTC
CTTCGCTACCGCAGCAGAAGCACTCACTCTCCATGGCACAACACTTGATG
GCAGAGAACTGGCAGTGTCA
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