mRNA_M-pyrifera_M_contig13392.5947.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig13392.5947.1
Unique NamemRNA_M-pyrifera_M_contig13392.5947.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig13392.5947.1 vs. uniprot
Match: D8LH20_ECTSI (Fe2OG dioxygenase domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LH20_ECTSI)

HSP 1 Score: 173 bits (438), Expect = 4.090e-51
Identity = 79/94 (84.04%), Postives = 88/94 (93.62%), Query Frame = 1 
Query:    1 TGCVMDMRHPDGVRVKNVYLPRGSLLVMEGAARYEWSHGIASRKTDMVKGVLTKRSTRVSLTFRRVRDPSVACPCNFEESCDRNTPVATATYLK 282
            +GCVMDMRHPDGVRVKN+YLPRGSLLVMEG ARYEWSHGIASRKTDMV GVLT+R+TR+S TFRRVRDP+VAC C+F +SCDR TPVATAT+LK
Sbjct:  255 SGCVMDMRHPDGVRVKNLYLPRGSLLVMEGPARYEWSHGIASRKTDMVDGVLTRRATRISFTFRRVRDPAVACGCDFAKSCDRETPVATATFLK 348
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BLAST of mRNA_M-pyrifera_M_contig13392.5947.1 vs. uniprot
Match: L8H3U3_ACACA (Alkylated DNA repair protein alkB homolog 8 n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8H3U3_ACACA)

HSP 1 Score: 84.3 bits (207), Expect = 7.620e-17
Identity = 45/83 (54.22%), Postives = 52/83 (62.65%), Query Frame = 1 
Query:    1 TGCVMDMRHPDGVRVKNVYLPRGSLLVMEGAARYEWSHGIASRKTDMVKGVLTKRS-TRVSLTFRRVRDPSVACPCNFEESCD 246
            + CVM +  PD  + K+V+LPR SLLVM GAARY+W H I  R+ D V G L KR   RVS TFRRVR     C C F E CD
Sbjct:  292 SSCVMTLSLPDSSQHKSVFLPRRSLLVMTGAARYQWRHAIPFRRYDCVHGQLIKREGKRVSFTFRRVRGHP--CDCRFAEECD 372
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BLAST of mRNA_M-pyrifera_M_contig13392.5947.1 vs. uniprot
Match: A0A8J2S925_9STRA (Hypothetical protein n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2S925_9STRA)

HSP 1 Score: 81.6 bits (200), Expect = 1.940e-16
Identity = 46/101 (45.54%), Postives = 59/101 (58.42%), Query Frame = 1 
Query:    4 GCVMDM----RHPDGVRVKNVYLPRGSLLVMEGAARYEWSHGIASRKTDMVKGVLTKRSTRVSLTFRRVRDPSVACPCNFEESCDRN--TPVATATYLKGS 288
            GC + +    RH  G  +  ++LP  S LV+ GAARY ++HGI SRK D+V G    R  RVSLTFRR+  P   C C+F ESCD +   P    T L+GS
Sbjct:  145 GCAIRLQRNRRHEAGAPIHTLWLPPRSALVLSGAARYVYTHGIVSRKGDLVDGEWRLRGRRVSLTFRRLPPPG-PCACSFPESCDASGTAPKLLPTRLRGS 244
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BLAST of mRNA_M-pyrifera_M_contig13392.5947.1 vs. uniprot
Match: K8YMX3_NANGC (2og-fe oxygenase (Fragment) n=1 Tax=Nannochloropsis gaditana (strain CCMP526) TaxID=1093141 RepID=K8YMX3_NANGC)

HSP 1 Score: 80.9 bits (198), Expect = 6.400e-16
Identity = 39/71 (54.93%), Postives = 50/71 (70.42%), Query Frame = 1 
Query:   49 NVYLPRGSLLVMEGAARYEWSHGIASRKTDMVKGVLTKRSTRVSLTFRRVRDPSVA-CPCNFEESCDRNTP 258
            ++ LPR SLLV++G ARY W+HG+ASRKTD V G L  R  RVSL+FRR+R+ +   C C F  SCD+  P
Sbjct:  261 SLLLPRRSLLVLQGEARYNWTHGLASRKTDKVNGRLVSRGRRVSLSFRRLRNGNPPLCECLFPASCDKRIP 331
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BLAST of mRNA_M-pyrifera_M_contig13392.5947.1 vs. uniprot
Match: W7TM49_9STRA (Alkylated dna repair protein alkb-like protein n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7TM49_9STRA)

HSP 1 Score: 80.9 bits (198), Expect = 1.090e-15
Identity = 39/71 (54.93%), Postives = 50/71 (70.42%), Query Frame = 1 
Query:   49 NVYLPRGSLLVMEGAARYEWSHGIASRKTDMVKGVLTKRSTRVSLTFRRVRDPSVA-CPCNFEESCDRNTP 258
            ++ LPR SLLV++G ARY W+HG+ASRKTD V G L  R  RVSL+FRR+R+ +   C C F  SCD+  P
Sbjct:  275 SLLLPRRSLLVLQGEARYNWTHGLASRKTDKVNGRLVSRGRRVSLSFRRLRNGNPPLCECLFPASCDKRIP 345
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BLAST of mRNA_M-pyrifera_M_contig13392.5947.1 vs. uniprot
Match: UPI001884B311 (alkylated DNA repair protein alkB homolog 8-like n=1 Tax=Pollicipes pollicipes TaxID=41117 RepID=UPI001884B311)

HSP 1 Score: 79.3 bits (194), Expect = 4.380e-15
Identity = 45/88 (51.14%), Postives = 50/88 (56.82%), Query Frame = 1 
Query:   22 RHPDGVRVKNVYLPRGSLLVMEGAARYEWSHGIASRKTDMVKGV-----LTKRSTRVSLTFRRVRDPSVACPCNFEESCDRNTPVATA 270
            R P G     V LPR S LVM+G ARY WSHGI  RK+D+  GV     LT R TRVSLT RR+R    AC C   + CD   P A A
Sbjct:  243 RPPPGGATAAVRLPRRSALVMDGEARYLWSHGIVPRKSDVTAGVGGDLTLTPRGTRVSLTLRRLR--RAACHCRHPQQCDSRQPAADA 328
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BLAST of mRNA_M-pyrifera_M_contig13392.5947.1 vs. uniprot
Match: A0A7R9VHP1_9CHLO (Hypothetical protein (Fragment) n=1 Tax=Chlamydomonas euryale TaxID=1486919 RepID=A0A7R9VHP1_9CHLO)

HSP 1 Score: 77.4 bits (189), Expect = 5.790e-15
Identity = 40/83 (48.19%), Postives = 49/83 (59.04%), Query Frame = 1 
Query:   31 DGVRVKNVYLPRGSLLVMEGAARYEWSHGIASRKTDMVKGVLTKRSTRVSLTFRRVRDPSVACPCNFEESCDRNTPVATATYL 279
            D  + + + LPR SLL+M G ARY W HGI +R+TDMV G    R  RVSLT R+VR    AC C + ESCD  +     T L
Sbjct:  156 DASQRRLLLLPRRSLLIMTGEARYAWRHGILARRTDMVGGAALPRDRRVSLTLRKVR--GFACNCEYPESCDSQSAPLQPTRL 236
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BLAST of mRNA_M-pyrifera_M_contig13392.5947.1 vs. uniprot
Match: UPI0015A8EAB5 (alkylated DNA repair protein alkB homolog 8 n=1 Tax=Anguilla anguilla TaxID=7936 RepID=UPI0015A8EAB5)

HSP 1 Score: 78.6 bits (192), Expect = 8.250e-15
Identity = 46/99 (46.46%), Postives = 54/99 (54.55%), Query Frame = 1 
Query:   10 VMDMRHPDGVRVKNVYLPRGSLLVMEGAARYEWSHGIASRKTDMVKG----------------VLTKRSTRVSLTFRRVRDPSVACPCNFEESCDRNTP 258
            VMD RHPDG  V  V LP  SLLVM+G +RY W+HGI  RK D+V                   L+KR TR S TFRRVR   +AC C +  +CD   P
Sbjct:  261 VMDFRHPDGRSVA-VVLPERSLLVMKGESRYLWTHGITPRKFDVVPAGDTVSSGPVTSDPGDLTLSKRDTRTSFTFRRVR--RMACDCAYPSACDSRRP 356
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BLAST of mRNA_M-pyrifera_M_contig13392.5947.1 vs. uniprot
Match: A0A6U3ZEE3_9STRA (Hypothetical protein n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A6U3ZEE3_9STRA)

HSP 1 Score: 74.3 bits (181), Expect = 9.870e-15
Identity = 35/65 (53.85%), Postives = 44/65 (67.69%), Query Frame = 1 
Query:   40 RVKNVYLPRGSLLVMEGAARYEWSHGIASRKTDMVKGVLTKRSTRVSLTFRRVRDPSVACPCNFE 234
            R K ++L R SLLV+ G ARY+WSHGIA R+ D V G L +R+TR+S TFR V+ P   C C  E
Sbjct:   29 RNKLLWLKRRSLLVLNGEARYDWSHGIAPRRLDRVGGDLRRRTTRISFTFRYVQGPGAKCQCGCE 93
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BLAST of mRNA_M-pyrifera_M_contig13392.5947.1 vs. uniprot
Match: A0A553QPZ4_9TELE (Fe2OG dioxygenase domain-containing protein n=1 Tax=Danionella translucida TaxID=623744 RepID=A0A553QPZ4_9TELE)

HSP 1 Score: 77.4 bits (189), Expect = 2.010e-14
Identity = 46/104 (44.23%), Postives = 58/104 (55.77%), Query Frame = 1 
Query:   10 VMDMRHPDGVRVKNVYLPRGSLLVMEGAARYEWSHGIASRKTDMV-------KGVLT---------KRSTRVSLTFRRVRDPSVACPCNFEESCDRNTPVATAT 273
            VMD +HPDG R   V LP  SLLVM+G +RY W+HGI  RKTD+V        GV+T         +R TR SLTFR++R     C C++   CD   P +  T
Sbjct:  111 VMDFKHPDG-RCVAVVLPERSLLVMKGESRYLWTHGITPRKTDVVPVSETRGSGVMTSDLSNLTLSRRETRTSLTFRKIRRHP--CKCSYPSVCDSQLPGSPPT 211
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig13392.5947.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LH20_ECTSI4.090e-5184.04Fe2OG dioxygenase domain-containing protein n=2 Ta... [more]
L8H3U3_ACACA7.620e-1754.22Alkylated DNA repair protein alkB homolog 8 n=1 Ta... [more]
A0A8J2S925_9STRA1.940e-1645.54Hypothetical protein n=1 Tax=Pelagomonas calceolat... [more]
K8YMX3_NANGC6.400e-1654.932og-fe oxygenase (Fragment) n=1 Tax=Nannochloropsi... [more]
W7TM49_9STRA1.090e-1554.93Alkylated dna repair protein alkb-like protein n=1... [more]
UPI001884B3114.380e-1551.14alkylated DNA repair protein alkB homolog 8-like n... [more]
A0A7R9VHP1_9CHLO5.790e-1548.19Hypothetical protein (Fragment) n=1 Tax=Chlamydomo... [more]
UPI0015A8EAB58.250e-1546.46alkylated DNA repair protein alkB homolog 8 n=1 Ta... [more]
A0A6U3ZEE3_9STRA9.870e-1553.85Hypothetical protein n=1 Tax=Dictyocha speculum Ta... [more]
A0A553QPZ4_9TELE2.010e-1444.23Fe2OG dioxygenase domain-containing protein n=1 Ta... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig13392contigM-pyrifera_M_contig13392:6448..8705 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score165.6
Seed ortholog evalue1.2e-38
Seed eggNOG ortholog2880.D8LH20
Preferred nameALKBH8
KEGG koko:K10770,ko:K14685,ko:K17867,ko:K18670
KEGG TC2.A.100.1
KEGG Pathwayko04216,ko04978,map04216,map04978
Hectar predicted targeting categoryother localisation
GOsGO:0000049,GO:0001510,GO:0002097,GO:0002098,GO:0003674,GO:0003676,GO:0003723,GO:0003824,GO:0005488,GO:0005506,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005737,GO:0005829,GO:0006139,GO:0006396,GO:0006399,GO:0006400,GO:0006725,GO:0006807,GO:0006950,GO:0006974,GO:0008033,GO:0008150,GO:0008152,GO:0008168,GO:0008173,GO:0008175,GO:0008198,GO:0008270,GO:0009451,GO:0009987,GO:0010467,GO:0016070,GO:0016300,GO:0016491,GO:0016604,GO:0016705,GO:0016706,GO:0016740,GO:0016741,GO:0030488,GO:0031974,GO:0031981,GO:0032259,GO:0033554,GO:0034470,GO:0034641,GO:0034660,GO:0043167,GO:0043169,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043412,GO:0043414,GO:0044237,GO:0044238,GO:0044260,GO:0044422,GO:0044424,GO:0044428,GO:0044444,GO:0044446,GO:0044451,GO:0044464,GO:0046483,GO:0046872,GO:0046914,GO:0050896,GO:0051213,GO:0051716,GO:0055114,GO:0070013,GO:0071704,GO:0090304,GO:0097159,GO:0140098,GO:0140101,GO:1901360,GO:1901363
EggNOG free text desc.dioxygenase activity
EggNOG OGsCOG3145@1,KOG4176@2759
EC2.1.1.229,2.7.12.1
COG Functional cat.L
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko01001,ko02000,ko03012,ko03016
Exons2
Model size312
Cds size300
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig13392.5947.1prot_M-pyrifera_M_contig13392.5947.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig13392 6448..8693 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815816.151863-CDS-M-pyrifera_M_contig13392:6447..66181622815816.151863-CDS-M-pyrifera_M_contig13392:6447..6618Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig13392 6448..6618 -
1692277584.9357376-CDS-M-pyrifera_M_contig13392:6447..66181692277584.9357376-CDS-M-pyrifera_M_contig13392:6447..6618Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig13392 6448..6618 -
1622815816.16288-CDS-M-pyrifera_M_contig13392:8564..86931622815816.16288-CDS-M-pyrifera_M_contig13392:8564..8693Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig13392 8565..8693 -
1692277584.953217-CDS-M-pyrifera_M_contig13392:8564..86931692277584.953217-CDS-M-pyrifera_M_contig13392:8564..8693Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig13392 8565..8693 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815816.175764-UTR-M-pyrifera_M_contig13392:8693..87051622815816.175764-UTR-M-pyrifera_M_contig13392:8693..8705Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig13392 8694..8705 -
1692277584.9647458-UTR-M-pyrifera_M_contig13392:8693..87051692277584.9647458-UTR-M-pyrifera_M_contig13392:8693..8705Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig13392 8694..8705 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig13392.5947.1

>prot_M-pyrifera_M_contig13392.5947.1 ID=prot_M-pyrifera_M_contig13392.5947.1|Name=mRNA_M-pyrifera_M_contig13392.5947.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=100bp
MDMRHPDGVRVKNVYLPRGSLLVMEGAARYEWSHGIASRKTDMVKGVLTK
RSTRVSLTFRRVRDPSVACPCNFEESCDRNTPVATATYLKGSMDNDSNN*
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mRNA from alignment at M-pyrifera_M_contig13392:6448..8705-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig13392.5947.1 ID=mRNA_M-pyrifera_M_contig13392.5947.1|Name=mRNA_M-pyrifera_M_contig13392.5947.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=2258bp|location=Sequence derived from alignment at M-pyrifera_M_contig13392:6448..8705- (Macrocystis pyrifera P11B4 male)
ACAGGATGTGTCATGGACATGAGACATCCAGACGGCGTGCGCGTGAAGAA
CGTATATCTTCCGCGTGGTTCGTTGCTGGTTATGGAGGGTGCGGCGCGGT
ACGAATGGTCGCATGGCATCGCAAGTCGCAAGACTGACATGGTGCGTGAT
GCTTGCTTATTCCAATAAAGTCTAGCCCCACCGCTATGCCAGATACAGAG
GGGACCATTTTCCGTTAAACCCATCGAAACTGGTGTTGGTTTTCTACATT
TGCATCTATAGTTACTATCCATTAAAAAATATTTTACCTGCTCGTATGCG
TACCAGGTCGTTTAGAGCTTTCTATGTAAACGTGTTGTACTACAGTAGAT
AACAGAAAAAAATTGGAACCGACATAAAAAATCAAAAGGTACTTTTTTCG
GGTCGCCCTGAAAGGGTCGGCCACCGCATCGCGAGCGTGCTCAGATGCTT
GTGGCCGCTTTTTTGTGAGCTGGAGGCCTGGCCGCGTGTAGATTCGAGCT
TTCGCTGCCTAGGGTATTGCTTCGAGGTATTCTGCCTCCTTTTGATTCTG
CGCGGTGAATCCTGCGACGGCGTGGGCGGGGGCAGCTTGCCCCGTATGGC
ACTGCTGCTGCTGTACGTCTGGTTTGCCGCTGTTTTTATAAATACTTGCC
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACACCCAAAACCCCC
AAAAAAAAACAAACCACCCCCCCCACACCACAAACCCCCCCCACCACCCA
ATCTTTTCTTCTCATTNNNNNNNNNNCTCGGTCCTTGCGCTGTCCTGTCT
TCTGTTTTGCGCTGGTTTTTTAAAAACATTCCCCCCCCCCCCCCCCCCGC
CCCGCCCGTCGAACAATAACAAATGCCAATGCGAACATCAGGAAAACCTG
TCGACAAACCAATCCCACCCTCATGGTGTTTTTTTCTTCCAGATTTTTTT
GCCGAGGTTGTGATTGGGCAAATGTAACCTGTTCGAATGCCTCCGTCCTG
TTTGGCGAGACGGATGCCGACCCAGGTTTCGATGGAGGATTTTGTGCTCT
TCGCGCAAACGTACAGTCCCGCCCCGAAGCCTACGCCCCACAGGGCTCCT
AACTTTCTGAAATGATGTAAAATCATCCACACCTCCGACAGATCATCGAT
TCCCGCTAAATTATGTACTGTAGCTCTTACAAAGCAGATAAATAGTACAT
TCCCGACCTTGTATGGTTTGCATCATCTATGCTGCCGGGTGAGACCCGTT
TAATCTGTGCATGGTCTTTCGTGGCACATGTTCCCCCTGGGTTAGATCTG
TACTATGCGGCGCCGATCCTGCACAACCTCTCACAACGGCAGCTAAGGAA
CTATTACGGGGGAACACAGTATTCAGGACCAAATGTGCTTAGTAAAAATA
CTGGAATGTATAGGTTTTGGTGTATAGGGTGGGTCCTGATTACTGTGGTC
CCCCGTGATAGATGATCTAGATCATGATCTGTCCCAGGCGCGAGATCATT
CCTCCAGCTCAGATTTCTTCGCCTCCTTTCTACGCATGGGCAACAGCCAA
TCGATCCGTGTTCTCACCGCTTCCCCCCTCTCATCGCGTGCGGGCATACT
GCTGCTCAAGTCTTGGAGCACGATTGCACCTGCGGTGAAGGCCACGCACT
ACAATAGTACTACTGGAGGACTACTACTACTACAGTACTACTACTAGTTC
TGTTACTGCCGCTATCACCGCTATTGCCACTGCTACTAGAATCTACCTCG
GCTGTATATCCTACCCCTACGATGTTATGATATACTACTACCACTTCTGC
TGCTACTACTACCACTACTACTATTAATACTGCTTAATACTACTACTACT
GCTACTACACTACTACTACTAAATTACTCCTACTGCTACTACTTGTACTG
CTGTAGTTGTACTACAGTACTACTAAATACTACTATTAATACTACTACTA
CCACTGCTACTACTACTGCTACTGCTACTGCTACTGCTACTGCTACTGCC
GTAGATATGCGTAAGACCTCCTTTTTCCAAGCAGATATAACACCCCCCCC
TCCTCACGGCACCATTCCATTCCACTGTGACGGGCAGGTCAAGGGAGTAC
TGACGAAACGATCGACGCGGGTTTCGTTGACGTTTAGGCGTGTTCGCGAC
CCGTCAGTAGCGTGCCCGTGCAACTTCGAGGAGAGCTGCGACCGAAATAC
ACCCGTGGCCACCGCTACCTACCTCAAGGGATCGATGGACAATGACAGTA
ATAACTAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig13392:6448..8705-

>mRNA_M-pyrifera_M_contig13392.5947.1 ID=mRNA_M-pyrifera_M_contig13392.5947.1|Name=mRNA_M-pyrifera_M_contig13392.5947.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=600bp|location=Sequence derived from alignment at M-pyrifera_M_contig13392:6448..8705- (Macrocystis pyrifera P11B4 male)
ATGGACATGAGACATCCAGACGGCGTGCGCGTGAAGAACGTATATCTTCC
GCGTGGTTCGTTGCTGGTTATGGAGGGTGCGGCGCGGTACGAATGGTCGC
ATGGCATCGCAAGTCGCAAGACTGACATGATGGACATGAGACATCCAGAC
GGCGTGCGCGTGAAGAACGTATATCTTCCGCGTGGTTCGTTGCTGGTTAT
GGAGGGTGCGGCGCGGTACGAATGGTCGCATGGCATCGCAAGTCGCAAGA
CTGACATGGTCAAGGGAGTACTGACGAAACGATCGACGCGGGTTTCGTTG
ACGTTTAGGCGTGTTCGCGACCCGTCAGTAGCGTGCCCGTGCAACTTCGA
GGAGAGCTGCGACCGAAATACACCCGTGGCCACCGCTACCTACCTCAAGG
GATCGATGGACAATGACAGTAATAACTAGGTCAAGGGAGTACTGACGAAA
CGATCGACGCGGGTTTCGTTGACGTTTAGGCGTGTTCGCGACCCGTCAGT
AGCGTGCCCGTGCAACTTCGAGGAGAGCTGCGACCGAAATACACCCGTGG
CCACCGCTACCTACCTCAAGGGATCGATGGACAATGACAGTAATAACTAG
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