mRNA_M-pyrifera_M_contig13384.5942.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig13384.5942.1
Unique NamemRNA_M-pyrifera_M_contig13384.5942.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig13384.5942.1 vs. uniprot
Match: A0A6H5KVX7_9PHAE (PCI domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KVX7_9PHAE)

HSP 1 Score: 179 bits (453), Expect = 7.540e-51
Identity = 84/90 (93.33%), Postives = 87/90 (96.67%), Query Frame = 1 
Query:    7 QTANRLRGWEGWTNDGIELHLQTRFAQLEVAAGLELWTEGFRTVEDIYNIMQIGKKTPKAKLMASYYEKLTRIFWVSENYLFHAYAWSVF 276
            QT NRLRGWEGWT+DGIELHLQTRFAQLEVAAGLELWTEGFRTVEDIYNIMQIGKKTPKA+LMASYYEKLTRIFWVSEN+LFHAYAW  F
Sbjct:   12 QTTNRLRGWEGWTSDGIELHLQTRFAQLEVAAGLELWTEGFRTVEDIYNIMQIGKKTPKARLMASYYEKLTRIFWVSENFLFHAYAWYKF 101
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BLAST of mRNA_M-pyrifera_M_contig13384.5942.1 vs. uniprot
Match: D7FZL6_ECTSI (Eukaryotic translation initiation factor 3 subunit A n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FZL6_ECTSI)

HSP 1 Score: 180 bits (457), Expect = 1.160e-50
Identity = 85/90 (94.44%), Postives = 87/90 (96.67%), Query Frame = 1 
Query:    7 QTANRLRGWEGWTNDGIELHLQTRFAQLEVAAGLELWTEGFRTVEDIYNIMQIGKKTPKAKLMASYYEKLTRIFWVSENYLFHAYAWSVF 276
            QT NRLRGWEGWT+DGIELHLQTRFAQLEVAAGLELWTEGFRTVEDIYNIMQIGKKTPKA+LMASYYEKLTRIFWVSENYLFHAYAW  F
Sbjct:  222 QTTNRLRGWEGWTSDGIELHLQTRFAQLEVAAGLELWTEGFRTVEDIYNIMQIGKKTPKARLMASYYEKLTRIFWVSENYLFHAYAWYKF 311
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BLAST of mRNA_M-pyrifera_M_contig13384.5942.1 vs. uniprot
Match: A0A836CG33_9STRA (Eukaryotic translation initiation factor 3 subunit A n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CG33_9STRA)

HSP 1 Score: 162 bits (411), Expect = 1.850e-44
Identity = 77/85 (90.59%), Postives = 81/85 (95.29%), Query Frame = 1 
Query:   13 ANRLRGWEGWTNDGIELHLQTRFAQLEVAAGLELWTEGFRTVEDIYNIMQIGKKTPKAKLMASYYEKLTRIFWVSENYLFHAYAW 267
            A RLRGWEGWT++GIELHLQTRFAQLEVAA LELWTEGFRTVEDIYNIMQIGKK PKA+LMASYYEKLTRIF VSEN+LFHAYAW
Sbjct:  222 ATRLRGWEGWTSEGIELHLQTRFAQLEVAANLELWTEGFRTVEDIYNIMQIGKKVPKARLMASYYEKLTRIFLVSENHLFHAYAW 306
[Close]
BLAST of mRNA_M-pyrifera_M_contig13384.5942.1 vs. uniprot
Match: F0XXZ4_AURAN (PCI domain-containing protein (Fragment) n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0XXZ4_AURAN)

HSP 1 Score: 147 bits (372), Expect = 1.500e-39
Identity = 68/89 (76.40%), Postives = 79/89 (88.76%), Query Frame = 1 
Query:    1 TNQTANRLRGWEGWTNDGIELHLQTRFAQLEVAAGLELWTEGFRTVEDIYNIMQIGKKTPKAKLMASYYEKLTRIFWVSENYLFHAYAW 267
            TN T+ RLRGWEGWT +G+E+HL TRFAQLEVA+ LELWTEGFRTVEDI+ IM+I KK PKAKLMA YYE+LT+IFWVS N+LFHA+AW
Sbjct:  214 TNNTS-RLRGWEGWTQEGVEMHLTTRFAQLEVASALELWTEGFRTVEDIHQIMRISKKPPKAKLMAKYYERLTKIFWVSGNHLFHAFAW 301
[Close]
BLAST of mRNA_M-pyrifera_M_contig13384.5942.1 vs. uniprot
Match: A0A7S3ZQV0_9STRA (Eukaryotic translation initiation factor 3 subunit A n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A7S3ZQV0_9STRA)

HSP 1 Score: 148 bits (374), Expect = 1.820e-39
Identity = 67/85 (78.82%), Postives = 74/85 (87.06%), Query Frame = 1 
Query:   13 ANRLRGWEGWTNDGIELHLQTRFAQLEVAAGLELWTEGFRTVEDIYNIMQIGKKTPKAKLMASYYEKLTRIFWVSENYLFHAYAW 267
             NRLRGWEGWT +GIELHL TRFAQLE A+  ELWTEGFRTVEDI+ +M+I KK PKAKLMA YYE+LT+IFWVS NYLFHAYAW
Sbjct:  222 TNRLRGWEGWTQEGIELHLATRFAQLEAASAQELWTEGFRTVEDIHQVMRISKKPPKAKLMAKYYERLTKIFWVSGNYLFHAYAW 306
[Close]
BLAST of mRNA_M-pyrifera_M_contig13384.5942.1 vs. uniprot
Match: A0A6V1NAU3_HETAK (Hypothetical protein (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6V1NAU3_HETAK)

HSP 1 Score: 141 bits (355), Expect = 1.100e-38
Identity = 63/86 (73.26%), Postives = 76/86 (88.37%), Query Frame = 1 
Query:   10 TANRLRGWEGWTNDGIELHLQTRFAQLEVAAGLELWTEGFRTVEDIYNIMQIGKKTPKAKLMASYYEKLTRIFWVSENYLFHAYAW 267
            TA RLRGW+G+T + +ELHLQTRFAQL+ A  LE+WTE FRT+EDIY+IMQI KK PKA+LMA+Y+E+LTRIFWVS+N LFHAYAW
Sbjct:  222 TAQRLRGWDGFTPESVELHLQTRFAQLKAATALEVWTEAFRTIEDIYSIMQINKKAPKARLMAAYFEQLTRIFWVSQNRLFHAYAW 307
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BLAST of mRNA_M-pyrifera_M_contig13384.5942.1 vs. uniprot
Match: A0A7S2R6Z3_9STRA (Eukaryotic translation initiation factor 3 subunit A n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2R6Z3_9STRA)

HSP 1 Score: 141 bits (355), Expect = 6.610e-37
Identity = 64/83 (77.11%), Postives = 72/83 (86.75%), Query Frame = 1 
Query:   19 RLRGWEGWTNDGIELHLQTRFAQLEVAAGLELWTEGFRTVEDIYNIMQIGKKTPKAKLMASYYEKLTRIFWVSENYLFHAYAW 267
            RL+ W+GWT++ IELHLQTRF QLE  A LELWTEGF+TVEDI+ IMQI KK PKA++MA YYEKL RIFWVSENYLFHAYAW
Sbjct:  227 RLKVWDGWTDESIELHLQTRFRQLETTAKLELWTEGFKTVEDIHGIMQINKKAPKARVMAMYYEKLQRIFWVSENYLFHAYAW 309
[Close]
BLAST of mRNA_M-pyrifera_M_contig13384.5942.1 vs. uniprot
Match: A0A1Z5KQK7_FISSO (Eukaryotic translation initiation factor 3 subunit A n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5KQK7_FISSO)

HSP 1 Score: 140 bits (354), Expect = 8.990e-37
Identity = 65/87 (74.71%), Postives = 76/87 (87.36%), Query Frame = 1 
Query:   13 ANRLRGWEGWTNDGIELHLQTRFAQLEVAAGLELWTEGFRTVEDIYNIMQIG--KKTPKAKLMASYYEKLTRIFWVSENYLFHAYAW 267
            +N++RGWEGW+ D +ELHLQTRFAQLE A+ L  +TEGFRTVEDIYNI+QIG  K  PKAKLMA+YYEKLT +FWVSENYLFHA+AW
Sbjct:  271 SNKIRGWEGWSQDSVELHLQTRFAQLETASVLHRYTEGFRTVEDIYNILQIGRGKTPPKAKLMAAYYEKLTTLFWVSENYLFHAFAW 357
[Close]
BLAST of mRNA_M-pyrifera_M_contig13384.5942.1 vs. uniprot
Match: A0A7S3PZF8_9STRA (Eukaryotic translation initiation factor 3 subunit A n=1 Tax=Chaetoceros debilis TaxID=122233 RepID=A0A7S3PZF8_9STRA)

HSP 1 Score: 136 bits (343), Expect = 2.750e-35
Identity = 65/96 (67.71%), Postives = 76/96 (79.17%), Query Frame = 1 
Query:   16 NRLRGWEGWTNDGIELHLQTRFAQLEVAAGLELWTEGFRTVEDIYNIMQI---------GKKTPKAKLMASYYEKLTRIFWVSENYLFHAYAWSVF 276
            NR+RGWEGWT++ IELHLQTRF QLE A+ L L+TEGFRTVEDI+NI+QI         G   PKAK+MA+YYEKLT +FWVSENYLFHA+AW  F
Sbjct:  236 NRVRGWEGWTSESIELHLQTRFTQLETASTLHLYTEGFRTVEDIFNILQISNSRRKVAGGSAAPKAKIMAAYYEKLTTLFWVSENYLFHAFAWYKF 331
[Close]
BLAST of mRNA_M-pyrifera_M_contig13384.5942.1 vs. uniprot
Match: A0A7S2WH75_9STRA (Eukaryotic translation initiation factor 3 subunit A n=1 Tax=Eucampia antarctica TaxID=49252 RepID=A0A7S2WH75_9STRA)

HSP 1 Score: 135 bits (341), Expect = 5.110e-35
Identity = 63/91 (69.23%), Postives = 75/91 (82.42%), Query Frame = 1 
Query:   16 NRLRGWEGWTNDGIELHLQTRFAQLEVAAGLELWTEGFRTVEDIYNIMQIGKKT-------PKAKLMASYYEKLTRIFWVSENYLFHAYAW 267
            N++RGWEGWT D +ELHLQTRFAQLE A+ L L+TEGFRTVEDIYNI++I +         P+AKLMA+YYEKLT +FWVSENYLFHA+AW
Sbjct:  257 NKIRGWEGWTMDSLELHLQTRFAQLETASTLSLYTEGFRTVEDIYNILEISQSNAKRLGGMPRAKLMATYYEKLTTLFWVSENYLFHAFAW 347
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig13384.5942.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KVX7_9PHAE7.540e-5193.33PCI domain-containing protein n=1 Tax=Ectocarpus s... [more]
D7FZL6_ECTSI1.160e-5094.44Eukaryotic translation initiation factor 3 subunit... [more]
A0A836CG33_9STRA1.850e-4490.59Eukaryotic translation initiation factor 3 subunit... [more]
F0XXZ4_AURAN1.500e-3976.40PCI domain-containing protein (Fragment) n=1 Tax=A... [more]
A0A7S3ZQV0_9STRA1.820e-3978.82Eukaryotic translation initiation factor 3 subunit... [more]
A0A6V1NAU3_HETAK1.100e-3873.26Hypothetical protein (Fragment) n=1 Tax=Heterosigm... [more]
A0A7S2R6Z3_9STRA6.610e-3777.11Eukaryotic translation initiation factor 3 subunit... [more]
A0A1Z5KQK7_FISSO8.990e-3774.71Eukaryotic translation initiation factor 3 subunit... [more]
A0A7S3PZF8_9STRA2.750e-3567.71Eukaryotic translation initiation factor 3 subunit... [more]
A0A7S2WH75_9STRA5.110e-3569.23Eukaryotic translation initiation factor 3 subunit... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig13384contigM-pyrifera_M_contig13384:369..662 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score180.6
Seed ortholog evalue3.4e-43
Seed eggNOG ortholog2880.D7FZL6
Preferred nameTIF32
KEGG koko:K03254
KEGG Pathwayko03013,map03013
Hectar predicted targeting categoryother localisation
GOsGO:0000131,GO:0001732,GO:0002181,GO:0002183,GO:0002188,GO:0003674,GO:0003676,GO:0003723,GO:0003729,GO:0003743,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005852,GO:0006412,GO:0006413,GO:0006518,GO:0006807,GO:0008135,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009987,GO:0010467,GO:0010494,GO:0016043,GO:0016282,GO:0019538,GO:0022607,GO:0022613,GO:0022618,GO:0032991,GO:0034622,GO:0034641,GO:0034645,GO:0035690,GO:0035770,GO:0036464,GO:0042221,GO:0042493,GO:0043043,GO:0043170,GO:0043226,GO:0043228,GO:0043229,GO:0043232,GO:0043603,GO:0043604,GO:0043614,GO:0043933,GO:0044085,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044271,GO:0044424,GO:0044444,GO:0044464,GO:0050896,GO:0051716,GO:0065003,GO:0070887,GO:0070993,GO:0071540,GO:0071541,GO:0071704,GO:0071826,GO:0071840,GO:0097159,GO:1901363,GO:1901564,GO:1901566,GO:1901576,GO:1990904
EggNOG free text desc.translation initiation factor activity
EggNOG OGsKOG2072@1,KOG2072@2759
COG Functional cat.J
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko03012
Exons1
Model size294
Cds size294
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815815.751533-CDS-M-pyrifera_M_contig13384:368..6621622815815.751533-CDS-M-pyrifera_M_contig13384:368..662Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig13384 369..662 -
1692277584.7316713-CDS-M-pyrifera_M_contig13384:368..6621692277584.7316713-CDS-M-pyrifera_M_contig13384:368..662Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig13384 369..662 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig13384.5942.1prot_M-pyrifera_M_contig13384.5942.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig13384 369..662 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig13384.5942.1

>prot_M-pyrifera_M_contig13384.5942.1 ID=prot_M-pyrifera_M_contig13384.5942.1|Name=mRNA_M-pyrifera_M_contig13384.5942.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=98bp
TNQTANRLRGWEGWTNDGIELHLQTRFAQLEVAAGLELWTEGFRTVEDIY
NIMQIGKKTPKAKLMASYYEKLTRIFWVSENYLFHAYAWSVFLFIYF*
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mRNA from alignment at M-pyrifera_M_contig13384:369..662-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig13384.5942.1 ID=mRNA_M-pyrifera_M_contig13384.5942.1|Name=mRNA_M-pyrifera_M_contig13384.5942.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=294bp|location=Sequence derived from alignment at M-pyrifera_M_contig13384:369..662- (Macrocystis pyrifera P11B4 male)
ACCAATCAGACTGCAAACCGCCTTCGCGGGTGGGAGGGGTGGACGAACGA
CGGCATTGAGCTTCACCTGCAGACCCGCTTCGCTCAGCTGGAGGTTGCCG
CGGGTCTTGAGCTGTGGACTGAAGGGTTCCGCACGGTGGAGGACATCTAC
AACATAATGCAGATCGGCAAAAAGACGCCCAAGGCGAAGCTGATGGCCAG
CTACTACGAGAAGCTTACGCGCATCTTCTGGGTCAGCGAGAACTACCTCT
TCCACGCCTACGCTTGGTCTGTTTTTTTATTTATTTATTTTTAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig13384:369..662-

>mRNA_M-pyrifera_M_contig13384.5942.1 ID=mRNA_M-pyrifera_M_contig13384.5942.1|Name=mRNA_M-pyrifera_M_contig13384.5942.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=588bp|location=Sequence derived from alignment at M-pyrifera_M_contig13384:369..662- (Macrocystis pyrifera P11B4 male)
ACCAATCAGACTGCAAACCGCCTTCGCGGGTGGGAGGGGTGGACGAACGA
CGGCATTGAGCTTCACCTGCAGACCCGCTTCGCTCAGCTGGAGGTTGCCG
CGGGTCTTGAGCTGTGGACTGAAGGGTTCCGCACGGTGGAGGACATCTAC
AACATAATGCAGATCGGCAAAAAGACGCCCAAGGCGAAGCTGATGGCCAG
CTACTACGAGAAGCTTACGCGCATCTTCTGGGTCAGCGAGAACTACCTCT
TCCACGCCTACGCTTGGTCTGTTTTTTTATTTATTTATTTTTAAACCAAT
CAGACTGCAAACCGCCTTCGCGGGTGGGAGGGGTGGACGAACGACGGCAT
TGAGCTTCACCTGCAGACCCGCTTCGCTCAGCTGGAGGTTGCCGCGGGTC
TTGAGCTGTGGACTGAAGGGTTCCGCACGGTGGAGGACATCTACAACATA
ATGCAGATCGGCAAAAAGACGCCCAAGGCGAAGCTGATGGCCAGCTACTA
CGAGAAGCTTACGCGCATCTTCTGGGTCAGCGAGAACTACCTCTTCCACG
CCTACGCTTGGTCTGTTTTTTTATTTATTTATTTTTAA
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