mRNA_M-pyrifera_M_contig127740.5588.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig127740.5588.1
Unique NamemRNA_M-pyrifera_M_contig127740.5588.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig127740.5588.1 vs. uniprot
Match: UPI001C3E5F10 (AMP-binding protein n=2 Tax=Miltoncostaea oceani TaxID=2843216 RepID=UPI001C3E5F10)

HSP 1 Score: 313 bits (802), Expect = 1.530e-102
Identity = 153/158 (96.84%), Postives = 158/158 (100.00%), Query Frame = 1 
Query:    1 EGYGLTETGMATYMDPTRPAVAGSMGKANPGYEVTIVEPGTDRPLPPDTPGEIVVDMKIPNIVMRAYYGMPEKTAEDFRNLKLHTGDLGRMDEDGYFYFMDRVKDYIRRRGENVSSMEVERQASDHPNIREAAAIGVKAGEGASSEDEIMIVCIPEGD 474
            EGYGLTETGMATYMDPT+PAVAGSMGKANPGYEVTIVEPGTDRPLPP+TPGEIVVDMKIPNIVMRAYYGMPEKTAEDFRNLKLHTGDLGRMDE+GYFYFMDRVKDYIRRRGENVSSMEVERQASDHPNI+EAAAIGVKAGEGASSEDEIMIVCIPEG+
Sbjct:  331 EGYGLTETGMATYMDPTKPAVAGSMGKANPGYEVTIVEPGTDRPLPPNTPGEIVVDMKIPNIVMRAYYGMPEKTAEDFRNLKLHTGDLGRMDEEGYFYFMDRVKDYIRRRGENVSSMEVERQASDHPNIKEAAAIGVKAGEGASSEDEIMIVCIPEGE 488
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BLAST of mRNA_M-pyrifera_M_contig127740.5588.1 vs. uniprot
Match: UPI001C3CF863 (AMP-binding protein n=1 Tax=Miltoncostaea marina TaxID=2843215 RepID=UPI001C3CF863)

HSP 1 Score: 292 bits (748), Expect = 2.010e-94
Identity = 145/158 (91.77%), Postives = 148/158 (93.67%), Query Frame = 1 
Query:    1 EGYGLTETGMATYMDPTRPAVAGSMGKANPGYEVTIVEPGTDRPLPPDTPGEIVVDMKIPNIVMRAYYGMPEKTAEDFRNLKLHTGDLGRMDEDGYFYFMDRVKDYIRRRGENVSSMEVERQASDHPNIREAAAIGVKAGEGASSEDEIMIVCIPEGD 474
            EGYGLTETG+AT MDPTRP   GS G ANPGYEVTIVEPGTD+PLPPDTPGEIVVDMKIPNIVMRAYYGMPEKTAEDFRNLKLHTGDLGRMD DGY YFMDRVKDYIRRRGENVSSMEVERQ SDHP I+EAAAIGVKAGEGASSEDEIMIVCIPEGD
Sbjct:  331 EGYGLTETGVATMMDPTRPPRLGSCGVANPGYEVTIVEPGTDKPLPPDTPGEIVVDMKIPNIVMRAYYGMPEKTAEDFRNLKLHTGDLGRMDADGYVYFMDRVKDYIRRRGENVSSMEVERQVSDHPAIKEAAAIGVKAGEGASSEDEIMIVCIPEGD 488
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BLAST of mRNA_M-pyrifera_M_contig127740.5588.1 vs. uniprot
Match: UPI001C3D97C7 (AMP-binding protein n=1 Tax=Miltoncostaea oceani TaxID=2843216 RepID=UPI001C3D97C7)

HSP 1 Score: 286 bits (733), Expect = 3.890e-92
Identity = 139/156 (89.10%), Postives = 147/156 (94.23%), Query Frame = 1 
Query:    1 EGYGLTETGMATYMDPTRPAVAGSMGKANPGYEVTIVEPGTDRPLPPDTPGEIVVDMKIPNIVMRAYYGMPEKTAEDFRNLKLHTGDLGRMDEDGYFYFMDRVKDYIRRRGENVSSMEVERQASDHPNIREAAAIGVKAGEGASSEDEIMIVCIPE 468
            EGYGLTETG+AT MDPTRP   GS G ANPGYEVTIVEPGTDRPLPPDTPGEIVVDMK+PNIVMRAYYGMPEKTAEDFRNLKLHTGDLGRMDEDGY YFMDRVKDYIRRRGENVSSMEVE+Q +DHP+++EAAAIGVKAGEGASSEDEIMIVC+ E
Sbjct:  333 EGYGLTETGVATLMDPTRPPRLGSCGTANPGYEVTIVEPGTDRPLPPDTPGEIVVDMKVPNIVMRAYYGMPEKTAEDFRNLKLHTGDLGRMDEDGYVYFMDRVKDYIRRRGENVSSMEVEKQVADHPSVKEAAAIGVKAGEGASSEDEIMIVCVAE 488
[Close]
BLAST of mRNA_M-pyrifera_M_contig127740.5588.1 vs. uniprot
Match: A0A6L5F485_9PSEU (AMP-binding protein (Fragment) n=1 Tax=Actinophytocola sp. TaxID=1872138 RepID=A0A6L5F485_9PSEU)

HSP 1 Score: 269 bits (688), Expect = 9.420e-90
Identity = 134/154 (87.01%), Postives = 142/154 (92.21%), Query Frame = 1 
Query:    1 EGYGLTETGMATYMDPTRPAVAGSMGKANPGYEVTIVEPGTDRPLPPDTPGEIVVDMKIPNIVMRAYYGMPEKTAEDFRNLKLHTGDLGRMDEDGYFYFMDRVKDYIRRRGENVSSMEVERQASDHPNIREAAAIGVKAGEGASSEDEIMIVCI 462
            EGYGLTETGMATYMDPTRPAV GSMGKANPGYEVTIVEPGTDRPL P TPGEIVVDMKIPNIVMRAYYGM EKTAEDFRNLKLHTGDLGRMDE+GYFYFMDRVKDYIRRRGENVSSMEVE   + H ++ EAAA+GVKA EGAS+EDEI+ VC+
Sbjct:   41 EGYGLTETGMATYMDPTRPAVPGSMGKANPGYEVTIVEPGTDRPLEPGTPGEIVVDMKIPNIVMRAYYGMAEKTAEDFRNLKLHTGDLGRMDEEGYFYFMDRVKDYIRRRGENVSSMEVEAVVTGHADVLEAAAVGVKAAEGASAEDEIL-VCV 193
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BLAST of mRNA_M-pyrifera_M_contig127740.5588.1 vs. uniprot
Match: UPI001C3E22EB (AMP-binding protein n=1 Tax=Miltoncostaea marina TaxID=2843215 RepID=UPI001C3E22EB)

HSP 1 Score: 237 bits (605), Expect = 4.100e-73
Identity = 115/150 (76.67%), Postives = 130/150 (86.67%), Query Frame = 1 
Query:    1 EGYGLTETGMATYMDPTRPAVAGSMGKANPGYEVTIVEPGTDRPLPPDTPGEIVVDMKIPNIVMRAYYGMPEKTAEDFRNLKLHTGDLGRMDEDGYFYFMDRVKDYIRRRGENVSSMEVERQASDHPNIREAAAIGVKAGEGASSEDEIM 450
            EGYGLTETGM TY  P RP   GS G A PG+EV++VEPGTD P+PP TPGEIVVDMK+PNIVMR Y GMPEKTAEDFRNLKLHTGDLGRMDE+GY +F+DRVKDYIRRRGENVSS+EVE   + HP++ EAAA+GVKAGEGAS+EDEI+
Sbjct:  322 EGYGLTETGMVTYHPPGRPPRPGSCGIATPGFEVSVVEPGTDLPVPPGTPGEIVVDMKMPNIVMRTYAGMPEKTAEDFRNLKLHTGDLGRMDEEGYLWFLDRVKDYIRRRGENVSSVEVEHVLTSHPDVLEAAAVGVKAGEGASAEDEIL 471
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BLAST of mRNA_M-pyrifera_M_contig127740.5588.1 vs. uniprot
Match: UPI001C3D4971 (AMP-binding protein n=1 Tax=Miltoncostaea oceani TaxID=2843216 RepID=UPI001C3D4971)

HSP 1 Score: 234 bits (598), Expect = 4.620e-72
Identity = 113/155 (72.90%), Postives = 131/155 (84.52%), Query Frame = 1 
Query:    1 EGYGLTETGMATYMDPTRPAVAGSMGKANPGYEVTIVEPGTDRPLPPDTPGEIVVDMKIPNIVMRAYYGMPEKTAEDFRNLKLHTGDLGRMDEDGYFYFMDRVKDYIRRRGENVSSMEVERQASDHPNIREAAAIGVKAGEGASSEDEIMIVCIP 465
            EGYGLTETGM TY  P RP   GS G A PG+EV++V+P +D P+P  +PGEIVVDMK+PNIVMR+Y GMPEKTAEDFRNLKLHTGDLGR+DEDGY YF+DRVKDYIRRRGENVSSMEVER  + HP + EAAAIGV+A EGAS+EDEI++  +P
Sbjct:  322 EGYGLTETGMVTYHPPGRPGRPGSCGVATPGFEVSVVDPTSDMPVPVGSPGEIVVDMKLPNIVMRSYAGMPEKTAEDFRNLKLHTGDLGRLDEDGYLYFLDRVKDYIRRRGENVSSMEVERIVASHPEVLEAAAIGVQASEGASAEDEILVCLVP 476
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BLAST of mRNA_M-pyrifera_M_contig127740.5588.1 vs. uniprot
Match: A0A2E5ATS6_9BACT (Uncharacterized protein n=2 Tax=bacterium TaxID=1869227 RepID=A0A2E5ATS6_9BACT)

HSP 1 Score: 199 bits (506), Expect = 2.760e-58
Identity = 100/159 (62.89%), Postives = 113/159 (71.07%), Query Frame = 1 
Query:    1 EGYGLTETGMATYMDPTRPAVAGSMGKANPGYEVTIVEPGTDRPLPPDTPGEIVVDMKIPNIVMRAYYGMPEKTAEDFRNLKLHTGDLGRMDEDGYFYFMDRVKDYIRRRGENVSSMEVERQASDHPNIREAAAIGVKAGE-GASSEDEIMIVCIPEGD 474
            EGYGLTE G  T+M P  P   GS GK +PGYE+ IV P TD  LPPDTPGE+V+  +IPNI +  Y+ MPEKT  DFRN   HTGDL R D+DGY +FMDRVKDYIRRRGEN+SS EVE   S HP I EAAAI VK  E G  SEDE+MIV + E D
Sbjct:  346 EGYGLTEVGQCTFMRPGEPFRIGSCGKESPGYEIKIVNPDTDEELPPDTPGELVLRPRIPNICLHYYHKMPEKTVSDFRNFWFHTGDLCRKDDDGYIFFMDRVKDYIRRRGENISSFEVENMVSSHPKINEAAAIAVKVDEEGRYSEDEVMIVVVKEND 504
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BLAST of mRNA_M-pyrifera_M_contig127740.5588.1 vs. uniprot
Match: A0A424QHI7_9DELT (ATP-dependent acyl-CoA ligase n=4 Tax=Bacteria TaxID=2 RepID=A0A424QHI7_9DELT)

HSP 1 Score: 198 bits (503), Expect = 7.910e-58
Identity = 100/157 (63.69%), Postives = 113/157 (71.97%), Query Frame = 1 
Query:    1 EGYGLTETGMATYMDPTRPAVAGSMGKANPGYEVTIVEPGTDRPLPPDTPGEIVVDMKIPNIVMRAYYGMPEKTAEDFRNLKLHTGDLGRMDEDGYFYFMDRVKDYIRRRGENVSSMEVERQASDHPNIREAAAIGVKAGE-GASSEDEIMIVCIPE 468
            EGYGLTE G  T+M P  P   GS GK +PGYE+ IV P TD  LPP+TPGE+V+  +IPNI +  Y+ MPEKT  DFRN   HTGDL R DEDGY +FMDRVKDYIRRRGEN+SS EVE   S HPNI EAAAI VK  E G  SEDE+MIV + E
Sbjct:  346 EGYGLTEVGQCTFMRPGEPFRVGSCGKESPGYEIKIVNPETDEELPPNTPGELVLRPRIPNICLHYYHKMPEKTVSDFRNFWFHTGDLCRKDEDGYIFFMDRVKDYIRRRGENISSFEVENMVSSHPNISEAAAIAVKVSEEGRYSEDEVMIVVVLE 502
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BLAST of mRNA_M-pyrifera_M_contig127740.5588.1 vs. uniprot
Match: A0A2H6AKJ0_9BACT (Long-chain-fatty-acid--CoA ligase n=2 Tax=Bacteria TaxID=2 RepID=A0A2H6AKJ0_9BACT)

HSP 1 Score: 197 bits (502), Expect = 1.310e-57
Identity = 97/154 (62.99%), Postives = 115/154 (74.68%), Query Frame = 1 
Query:    1 EGYGLTETGMATYMDPTRPAVAGSMGKANPGYEVTIVEPGTDRPLPPDTPGEIVVDMKIPNIVMRAYYGMPEKTAEDFRNLKLHTGDLGRMDEDGYFYFMDRVKDYIRRRGENVSSMEVERQASDHPNIREAAAIGVKAGEGASSEDEIMIVCI 462
            EGYGLTETG  TYM P  P   GS GK +PGYE+ IV+P TD  LP    GEIVV  +IPNI++  Y+ MPEKT +DFRN   HTGD GRMD+DGY YF+DRVKDYIRRRGEN+SS EVE+  + HP + E+AAIGVKA EG  +EDE+MIV +
Sbjct:  353 EGYGLTETGQITYMRPGEPFRVGSCGKESPGYEIKIVDPETDEELPRGQVGEIVVRPRIPNIMLHYYHKMPEKTVQDFRNFWFHTGDAGRMDKDGYIYFVDRVKDYIRRRGENISSFEVEKIVNSHPAVAESAAIGVKAKEGGYAEDEVMIVVV 506
[Close]
BLAST of mRNA_M-pyrifera_M_contig127740.5588.1 vs. uniprot
Match: A0A2H6AJI2_9BACT (Long-chain-fatty-acid--CoA ligase n=2 Tax=Bacteria TaxID=2 RepID=A0A2H6AJI2_9BACT)

HSP 1 Score: 197 bits (501), Expect = 1.450e-57
Identity = 96/154 (62.34%), Postives = 115/154 (74.68%), Query Frame = 1 
Query:    1 EGYGLTETGMATYMDPTRPAVAGSMGKANPGYEVTIVEPGTDRPLPPDTPGEIVVDMKIPNIVMRAYYGMPEKTAEDFRNLKLHTGDLGRMDEDGYFYFMDRVKDYIRRRGENVSSMEVERQASDHPNIREAAAIGVKAGEGASSEDEIMIVCI 462
            EGYGLTETG+ TYM P  P   GS GK  PGYEV IV+P TD  LP    GEIVV  +IPNI++  Y+ MPEKT +DFRN   HTGD GRMD+DGY YF+DRVKDYIRRRGEN+SS EVE+    HP + EAAA+G+K+GEG  +EDE+M+V +
Sbjct:  339 EGYGLTETGVVTYMRPNEPFRIGSCGKEAPGYEVKIVDPETDEELPRGKVGEIVVRPRIPNIMLYYYHKMPEKTVQDFRNFWFHTGDAGRMDKDGYVYFVDRVKDYIRRRGENISSFEVEKIVCQHPAVLEAAAVGIKSGEGKFAEDEVMVVIV 492
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig127740.5588.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UPI001C3E5F101.530e-10296.84AMP-binding protein n=2 Tax=Miltoncostaea oceani T... [more]
UPI001C3CF8632.010e-9491.77AMP-binding protein n=1 Tax=Miltoncostaea marina T... [more]
UPI001C3D97C73.890e-9289.10AMP-binding protein n=1 Tax=Miltoncostaea oceani T... [more]
A0A6L5F485_9PSEU9.420e-9087.01AMP-binding protein (Fragment) n=1 Tax=Actinophyto... [more]
UPI001C3E22EB4.100e-7376.67AMP-binding protein n=1 Tax=Miltoncostaea marina T... [more]
UPI001C3D49714.620e-7272.90AMP-binding protein n=1 Tax=Miltoncostaea oceani T... [more]
A0A2E5ATS6_9BACT2.760e-5862.89Uncharacterized protein n=2 Tax=bacterium TaxID=18... [more]
A0A424QHI7_9DELT7.910e-5863.69ATP-dependent acyl-CoA ligase n=4 Tax=Bacteria Tax... [more]
A0A2H6AKJ0_9BACT1.310e-5762.99Long-chain-fatty-acid--CoA ligase n=2 Tax=Bacteria... [more]
A0A2H6AJI2_9BACT1.450e-5762.34Long-chain-fatty-acid--CoA ligase n=2 Tax=Bacteria... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig127740contigM-pyrifera_M_contig127740:3..476 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score142.5
Seed ortholog evalue1.6e-31
Seed eggNOG ortholog1034347.CAHJ01000011_gene2273
KEGG koko:K02182
Hectar predicted targeting categoryother localisation
EggNOG free text desc.AMP-binding enzyme C-terminal domain
EggNOG OGs1TPSX@1239,1ZFF9@1386,4HUBC@91061,COG0318@1,COG0318@2
EC6.2.1.48
COG Functional cat.IQ
Best tax levelBacillus
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000
Exons1
Model size474
Cds size447
Stop0
Start1
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815791.6327837-UTR-M-pyrifera_M_contig127740:2..291622815791.6327837-UTR-M-pyrifera_M_contig127740:2..29Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig127740 3..29 +
1692277571.5381515-UTR-M-pyrifera_M_contig127740:2..291692277571.5381515-UTR-M-pyrifera_M_contig127740:2..29Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig127740 3..29 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815791.643387-CDS-M-pyrifera_M_contig127740:29..4761622815791.643387-CDS-M-pyrifera_M_contig127740:29..476Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig127740 30..476 +
1692277571.5483487-CDS-M-pyrifera_M_contig127740:29..4761692277571.5483487-CDS-M-pyrifera_M_contig127740:29..476Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig127740 30..476 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig127740.5588.1prot_M-pyrifera_M_contig127740.5588.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig127740 30..476 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig127740.5588.1

>prot_M-pyrifera_M_contig127740.5588.1 ID=prot_M-pyrifera_M_contig127740.5588.1|Name=mRNA_M-pyrifera_M_contig127740.5588.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=149bp
MATYMDPTRPAVAGSMGKANPGYEVTIVEPGTDRPLPPDTPGEIVVDMKI
PNIVMRAYYGMPEKTAEDFRNLKLHTGDLGRMDEDGYFYFMDRVKDYIRR
RGENVSSMEVERQASDHPNIREAAAIGVKAGEGASSEDEIMIVCIPEGD
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mRNA from alignment at M-pyrifera_M_contig127740:3..476+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig127740.5588.1 ID=mRNA_M-pyrifera_M_contig127740.5588.1|Name=mRNA_M-pyrifera_M_contig127740.5588.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=474bp|location=Sequence derived from alignment at M-pyrifera_M_contig127740:3..476+ (Macrocystis pyrifera P11B4 male)
GAGGGCTACGGCCTGACCGAGACCGGCATGGCCACCTACATGGACCCCAC
CCGCCCGGCCGTCGCCGGCTCGATGGGCAAGGCCAACCCCGGATACGAGG
TCACGATCGTCGAGCCGGGCACGGACCGCCCGCTCCCGCCCGACACCCCG
GGTGAGATCGTCGTCGACATGAAGATCCCCAACATCGTCATGCGCGCCTA
CTACGGCATGCCCGAAAAGACCGCCGAGGACTTTCGCAACCTGAAGCTCC
ACACCGGAGACCTCGGGCGCATGGACGAAGACGGCTACTTCTACTTCATG
GACCGCGTCAAGGACTACATCCGCCGCCGCGGCGAGAACGTGTCCTCGAT
GGAGGTCGAGCGCCAGGCCTCGGACCACCCCAACATCCGCGAGGCGGCCG
CCATCGGCGTGAAGGCCGGCGAGGGCGCCTCCTCCGAGGACGAGATCATG
ATCGTGTGCATCCCCGAGGGCGAC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig127740:3..476+

>mRNA_M-pyrifera_M_contig127740.5588.1 ID=mRNA_M-pyrifera_M_contig127740.5588.1|Name=mRNA_M-pyrifera_M_contig127740.5588.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=894bp|location=Sequence derived from alignment at M-pyrifera_M_contig127740:3..476+ (Macrocystis pyrifera P11B4 male)
ATGGCCACCTACATGGACCCCACCCGCCCGGCCGTCGCCGGCTCGATGGG
CAAGGCCAACCCCGGATACGAGGTCACGATCGTCGAGCCGGGCACGGACC
GCCCGCTCCCGCCCGACACCCCGGGTGAGATCGTCGTCGACATGAAGATC
CCCAACATCGTCATGCGCGCCTACTACGGCATGCCCGAAAAGACCGCCGA
GGACTTTCGCAACCTGAAGCTCCACACCGGAGACCTCGGGCGCATGGACG
AAGACGGCTACTTCTACTTCATGGACCGCGTCAAGGACTACATCCGCCGC
CGCGGCGAGAACGTGTCCTCGATGGAGGTCGAGCGCCAGGCCTCGGACCA
CCCCAACATCCGCGAGGCGGCCGCCATCGGCGTGAAGGCCGGCGAGGGCG
CCTCCTCCGAGGACGAGATCATGATCGTGTGCATCCCCGAGGGCGACATG
GCCACCTACATGGACCCCACCCGCCCGGCCGTCGCCGGCTCGATGGGCAA
GGCCAACCCCGGATACGAGGTCACGATCGTCGAGCCGGGCACGGACCGCC
CGCTCCCGCCCGACACCCCGGGTGAGATCGTCGTCGACATGAAGATCCCC
AACATCGTCATGCGCGCCTACTACGGCATGCCCGAAAAGACCGCCGAGGA
CTTTCGCAACCTGAAGCTCCACACCGGAGACCTCGGGCGCATGGACGAAG
ACGGCTACTTCTACTTCATGGACCGCGTCAAGGACTACATCCGCCGCCGC
GGCGAGAACGTGTCCTCGATGGAGGTCGAGCGCCAGGCCTCGGACCACCC
CAACATCCGCGAGGCGGCCGCCATCGGCGTGAAGGCCGGCGAGGGCGCCT
CCTCCGAGGACGAGATCATGATCGTGTGCATCCCCGAGGGCGAC
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