mRNA_M-pyrifera_M_contig12773.5585.1 (mRNA) Macrocystis pyrifera P11B4 male

BLAST of mRNA_M-pyrifera_M_contig12773.5585.1 vs. uniprot
Match: D8LE29_ECTSI (Thiaminase II n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LE29_ECTSI)

HSP 1 Score: 581 bits (1497), Expect = 3.020e-202
Identity = 322/518 (62.16%), Postives = 374/518 (72.20%), Query Frame = 1

Query:    1 GSLCPGCLWKDCQLEAMRSLHHPWTLGLATGTTSRHSFQHYVAQDAYFLRAFVKAYAYALTKVEDEGDVRAFHSLIGSVLDELGLHASYATKWGLDIHNELKHAFPLWGESVYAEWIETYSSEEFETAAVLVETLLDAHSAAAEYPSLLSLYRKAMGLEYAFFAAQSQGTAEDDRPHHLTQGDDSSGSVQISSGG--SVTFQPGEKIGKGRGVFSGVQHRVALLCVDFDDTLTDGDTTSLLVKTAKGQRGTEDGQAEVDREWKVLTSLFLNKWNEVTEESLAAKVRSTALLYGEDLSGAVDRKGLEAMLQKLQTVDLDSVSRVSDSKVRMTRCLSRALASSFQTHVKTRGTRLAYRFAAGELKLPGFGGDSPAPVTSNDLLYDEHGVSTGDIAIKVSGSFGKHQQFLNLAATARETARGFEAVPRTMSVYVGDSVTDLLAMLDADVGIVVGTSDGDAFEIVARAFGIGIRPLAAAYGVLGETGGQGGGSLDE--GSTGRGSCIYRASGWPEIDVFLFG 1542
            GS  PG LW+DCQLEAMRSLHHPWTLGLATG TS  SF++YVAQDAYFLRAF KAYAYAL K EDE D+RAFHSLIGSVL+ELGLHASY+ KWG+D+ NEL+HAFP W ES YAEWIETYSSE+FE AA LVE+LLD +SA A+YPSL +LYRKAM LE+AFF AQ  G  E      L Q      SV   +GG   ++ QPG +   GRG F GV  R+ALLCVDFDDTLT+GDT SLLV+TAK QR T + + ++ REW +LT  FL KW+E  E+SL+ K+ +T    G   SGAVDR+GLE ML+KL  VDLDSVSRVSDSK  ++R LS  L  S  +   T G        AGE   P   GD PA V+SNDL++DEHGVSTG+I +K+ GSFGKHQ+FL+LAATARE+    E+ PR M+VYVGDSVTDLLAMLDADVGIVVG S    FE VARAFGI IRPLA+ Y  +  TG      L      +G G C+YRAS W EIDVFLFG
Sbjct:   11 GSTLPGSLWQDCQLEAMRSLHHPWTLGLATGKTSLASFKNYVAQDAYFLRAFAKAYAYALAKAEDEEDIRAFHSLIGSVLEELGLHASYSAKWGIDVANELRHAFPNWTESAYAEWIETYSSEDFEDAAGLVESLLDKNSAGADYPSLFALYRKAMELEFAFFDAQLDGGTE---AAMLEQ------SVGAEAGGRQGMSAQPGGQDAGGRGRFRGVHERLALLCVDFDDTLTEGDTISLLVETAKAQRETPEDRVDLGREWGLLTRTFLGKWSETIEDSLSTKLSTTCTTNG---SGAVDREGLELMLRKLGVVDLDSVSRVSDSKDVISRVLSETLLDSHSSSSSTTGIDAGGCVWAGETASPRLEGDCPASVSSNDLVFDEHGVSTGEILVKIPGSFGKHQRFLDLAATARESVASSESSPRLMTVYVGDSVTDLLAMLDADVGIVVGNSGS--FEKVARAFGIDIRPLASVYEAMDGTG-----ELPRTGAPSGSGQCVYRASQWAEIDVFLFG 509          

BLAST of mRNA_M-pyrifera_M_contig12773.5585.1 vs. uniprot
Match: A0A6H5J5C1_9PHAE (TENA_THI-4 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5J5C1_9PHAE)

HSP 1 Score: 506 bits (1303), Expect = 1.930e-171
Identity = 313/623 (50.24%), Postives = 366/623 (58.75%), Query Frame = 1

Query:   13 PGCLWKDCQLEAMRSLHHPWTLGLATGTTSRHSFQHYVAQDAYFLRAFVKAYAYALTKVEDEGDVRAFHSLIGSVLDELGLHASYATKWGLDIHN-------------------------------------------------------------------------------------------------------------ELKHAFPLWGESVYAEWIETYSSEEFETAAVLVETLLDAHSAAAEYPSLLSLYRKAMGLEYAFFAAQSQGTAEDDRPHHLTQGDDSSGSVQISSGG--SVTFQPGEKIGKGRGVFSGVQHRVALLCVDFDDTLTDGDTTSLLVKTAKGQRGTEDGQAEVDREWKVLTSLFLNKWNEVTEESLAAKVRSTALLYGEDLSGAVDRKGLEAMLQKLQTVDLDSVSRVSDSKVRMTRCLSRALASSFQTHVKTRGTRLAYRFAAGELKLPGFGGDSPAPVTSNDLLYDEHGVSTGDIAIKVSGSFGKHQQFLNLAATARETARGFEAVPRTMSVYVGDSVTDLLAMLDADVGIVVGTSDGDAFEIVARAFGIGIRPLAAAYGVLGETGGQGGGSLDEGST--GRGSCIYRASGWPEIDVFLFG 1542
            PG LW+DCQLEAMRSLHHPWTLGLATG TS  SF++YVAQDAYFLRAF KAYAYAL K EDE D+RAFHSLIGSVL+ELGLHASY+ KWG+D+ N                                                                                                             EL+ A P W ES YAEWIETYSSE+FE AA LVE+LLD +SA A+YPSL +L RKAM LE+AFF AQ  G  E      L Q      SV   +GG   ++ QPG K   GRG F GV  R+ALLCVDFDDTLT+GDTTSLLV+TAK QR T + + ++ REW +LT  FL+KW+E  E+SL+ K+ +TA       SGAVDR+GLE ML+KL+ VDLDS SRVSDSKVR    L+R +   F   +         +   GE   P   GD PA V+SNDL++DEHGVSTG+IA+K+ GSFGKHQ+FL+LAA ARE+    E+ PR M+VYVGDSVTDLLAMLDADVGIVVG S    FE VARAFGI I PLA+ Y  +      G G L       G G  +YRAS W EIDVFLFG
Sbjct:   12 PGSLWQDCQLEAMRSLHHPWTLGLATGKTSLASFKNYVAQDAYFLRAFAKAYAYALAKAEDEEDIRAFHSLIGSVLEELGLHASYSAKWGIDVANVRAKTTGVKLQIGSCTPLNFGLTGVWNVVHCRFASVKLHSDWAHLLYPINQGNKQMQVSAKELGERAMKRRSFAGLYLASLQIGLYRRQFHQCNVMTFADVLSSLFVAQELRDALPNWTESPYAEWIETYSSEDFEDAAGLVESLLDKNSAGADYPSLFALCRKAMELEFAFFDAQLDGVTE---AAMLEQ------SVGAEAGGRQGMSVQPGGKDAGGRGRFRGVHERLALLCVDFDDTLTEGDTTSLLVETAKAQRETPEDRVDLGREWVLLTRTFLSKWSETIEDSLSTKLSTTACTTNG--SGAVDREGLELMLRKLEVVDLDSASRVSDSKVRE---LAREIRRYFLAAI-------IVQREPGETASPRLEGDCPASVSSNDLVFDEHGVSTGEIAVKIPGSFGKHQRFLDLAAMARESVASSESSPRLMTVYVGDSVTDLLAMLDADVGIVVGNSGS--FERVARAFGIDILPLASVYEAM-----DGRGELPTTGAPLGSGQRVYRASQWTEIDVFLFG 606          

BLAST of mRNA_M-pyrifera_M_contig12773.5585.1 vs. uniprot
Match: A0A0G4GYE5_VITBC (TENA_THI-4 domain-containing protein n=3 Tax=Vitrella brassicaformis TaxID=1169539 RepID=A0A0G4GYE5_VITBC)

HSP 1 Score: 160 bits (404), Expect = 4.930e-39
Identity = 171/597 (28.64%), Postives = 244/597 (40.87%), Query Frame = 1

Query:   22 LWKDCQLEAMRSLHHPWTLGLATGTTSRHSFQHYVAQDAYFLRAFVKAYAYALTKVE--DEGDVRAFHSLIGSVLDELGLHASYATKWGLDIH----NELKHAFPLWGESV------------------------------------------YAEWIETYSSEEFETAAVLVETLLDAHSAA-----------AEYPSLLSLYRKAMGLEYAFFAAQSQGTAEDDRPHHLTQGDDSSGSVQISSGGSVTFQPGEKIGKGRGVFSGVQHRVALLCVDFDDTLTDGDTTSLLVKTAKGQRGTEDGQAEVDREWKVLTSLFLNKWNEVTEESLAAKVRSTALLYGEDLSGAVDRKGLEAMLQKLQTVDLDSVSRVSDSKVRMTRCLSRALASSFQTHVKTR-GTRLAYRFAAGELKLPGFGGDSPA--------------------------PVTSNDLLYDEHGV-STGDIAIKVSGSFGKHQQFLNLAATARETARGFEAVPRTMSVYVGDSVTDLLAMLDADVGIVV--GTSDGDAFEIVARAFGIGIRPLAAAYGVLGETGGQGGGSLDEGSTGRGSCIYRASGWPEIDVFLFGD 1545
            LW+ C+  A++SL+HPW L L     +R S+  ++ QDA+FL  F KAYA+AL K    D   +RAF++LIG VL EL LH S +T+W +D      NE    +  +   V                                          Y EW+ TYSS EF   A  VE LLD ++             A   +LL  Y  AM LEY FF AQ+                        +   S +  PG      RGV S        + +DFD T T  DTT LL + A       D   +V + W+ +   F +K+     E L  KVR                 GLEA L  L   +  +V+ VS++++     L        +  VKT    +   R A GE+       D PA                          P+T N   + E    +TG+I+  ++G+  K + F  L        R        +SV++GDSVTDLLA+L+AD+GIV+    +  +  + V   +GI ++PL  A  ++ + G Q   ++   S      IY A GW EI V LFG+
Sbjct:   53 LWQKCRRRALKSLYHPWVLRLGASVLARESYVEFMRQDAFFLEIFAKAYAHALCKCPPGDVEGIRAFNALIGGVLSELTLHQS-STEWAIDTSTFTPNEATVRYTSFLRDVGADQWTTTAMVLASMAPCMSLYAFIGKALREGVGEEGMAGHRYNEWLATYSSAEFAQDAARVEELLDRYTDPLLSLSGQVGEDAVRGNLLYYYGNAMDLEYGFFDAQALPPPS------------------CAPSASPSRHPGLAQ---RGVLS-------FVAIDFDGTCTQRDTTPLLHEVAAEVHSDPD---QVRQRWRAIEDAFFDKYRRKVGELLEGKVRD-------------GMNGLEAFLGGLGEFEKAAVADVSEARL-----LQGFTRDDVRRMVKTSLRQKWQVRDACGEVLSGLLSHDVPASIISVSWSQDMIHTILQDACGAPLSLPITCNSFSFREDDQRATGEISSHMTGAVDKLRAFRRLREEKGREGR--------LSVFIGDSVTDLLALLEADIGIVLKDALNKNNTLDKVISLYGIDVQPLVRAA-MIAQCG-QEAATVTPVSMPP-MTIYAADGWDEIGVMLFGN 588          

BLAST of mRNA_M-pyrifera_M_contig12773.5585.1 vs. uniprot
Match: A0A2V0PBE0_9CHLO (Thiamine phosphate synthase n=1 Tax=Raphidocelis subcapitata TaxID=307507 RepID=A0A2V0PBE0_9CHLO)

HSP 1 Score: 157 bits (398), Expect = 2.440e-37
Identity = 179/610 (29.34%), Postives = 245/610 (40.16%), Query Frame = 1

Query:   22 LWKDCQLEAMRSLHHPWTLGLATGTTSRHSFQHYVAQDAYFLRAFVKAYAYALTKVED---EGDVRAFHSLIGSVLDELGLHASYATKWGLDIHNELKHAFP--------------------------------LWG--------------ESVYAEWIETYSSEEFETAAVLVETLLDAHSAAAEYPSLLSLYRKAMGLEYAFFAAQSQGTAEDDRPHHLTQGDDSSGSVQISSGGSVTFQPGEKIGKGRGVFSGVQHRVALLCVDFDDTLTDGDTTSLL----VKTAKGQRGTEDG---QAEVDREWKVLTSLFLNKWNEVTEESLAAKVRSTALLYGEDLSGAVDRKGLEAMLQKLQTVDL-----------------DSVSRVSDSKVRMTRC---LSRALASSFQTHVKTRGTRLAYRFAAGELKLP----------GFGGD----------SPAP----VTSNDLLYDEHGVSTGDIAIKVSGSFGKHQQFLNLAATARETARGFEAVPRTMSVYVGDSVTDLLAMLDADVGIVVGTSDGDAFEIVARAF---GIGIRPLAAAYGVLGETGGQGGGSLDEGSTGRGSCIYRASGWPEIDVFLFG 1542
            LW +CQLEA R+L HP+ L L  GT  R +F+HYVAQDA+FL  F +AYA AL K  D   E  V     L+  V  EL LH SYA  WG+++ + L H  P                                L+G               + YA+WI  YSS E+    +  E LLD  +  A Y  L SLY++AM LE AFF+A                                   PG             +H V +L  DFDDT T+ DT   L    +  A  + G E+    +A +D     L   + +K   +  E L     + A        G  D  GL A +++L   D+                 D V+  + +      C   L+ AL +   THV +     +  F A  L LP          G  GD          +P P    + +N+L     G+STG +   V  +  K + F  L A     ARG        +V+VGDS TD+L +L AD GIVVG++     +++ RA    G  + PL AA       GG  GG+  +G       +Y A+ W EI  FLFG
Sbjct:   20 LWGECQLEAFRALQHPFVLALGLGTLPRDTFRHYVAQDAHFLHFFARAYAAALDKCADANAEAHV-VLQQLLMGVHMELRLHGSYARTWGVEL-DALDHVDPACQAYTDFLMATAEDPETTVAEVLASMVPCSRLYGFLGCALARAHPRQRGNPYADWISMYSSFEYLVHPMQKEALLDRLAGDAPYDRLRSLYKRAMQLEVAFFSAA----------------------------------PG---------IPAARH-VGMLVCDFDDTCTEKDTIGTLLSAAIDAAVKRAGPEEXXXLRARLDAVVARLVENYSSKHAALLAEILPEGASAAAA------EGGFDEAGLRAFVERLSDFDVQMNAVAVESGILAGLSRDDVAAAARAVALKPGCREALAAALGAELPTHVVS--VNWSREFVAAALGLPAADAADWGAAGSDGDEAAAAVIAGAAPPPGRLAIHANELARGADGLSTGALRRAVQCARDKGRLFAALQA-----ARGKGGXXXXAAVFVGDSATDVLPLLAADYGIVVGSN-----KLLRRALARHGASLVPLCAAPLTAPARGG--GGAASDGVA---RVVYEAASWDEIHAFLFG 560          

BLAST of mRNA_M-pyrifera_M_contig12773.5585.1 vs. uniprot
Match: D8T432_SELML (TENA_THI-4 domain-containing protein n=5 Tax=Selaginella moellendorffii TaxID=88036 RepID=D8T432_SELML)

HSP 1 Score: 148 bits (373), Expect = 4.070e-35
Identity = 150/564 (26.60%), Postives = 234/564 (41.49%), Query Frame = 1

Query:   22 LWKDCQLEAMRSLHHPWTLGLATGTTSRHSFQHYVAQDAYFLRAFVKAYAYALTKVEDEGDVRAFHSLIGSVLDELGLHASYATKWGLDIHNELKHA--------------------------------------FPLWGESV---------YAEWIETYSSEEFETAAVLVETLLDAHS-----AAAEYPSLLSLYRKAMGLEYAFFAAQSQGTAEDDRPHHLTQGDDSSGSVQISSGGSVTFQPG-EKIGKGRGVFSGVQHRVALLCVDFDDTLTDGDTTSLLVKTAKGQRGTEDGQAEVDREWKVLTSLFLNKWNEVTEESLAAKVRSTALLYGEDLSGAVDRKG---LEAMLQKLQTVDLDSVSRVSDSKVRMTRCLSRALASSFQTHVKTRGTRLAYRFAAGELKLPGFGGDSPAPVTSNDLLYDEHGVSTGDIAIKVSGSFGKHQQFLNLAATARETARGFEAVPRTMSVYVGDSVTDLLAMLDADVGIVVGTSDGDAFEIVARAFGIGIRPLAAAYGVLGETGGQGGGSLDEGSTGRGSCIYRASGWPEIDVFLFGD 1545
            LW+  + EAM  L+HP+ + +A GT   HSFQ ++AQD++FL AF KAY  A+ + +D         L  +V +EL LH+S    W  D H     A                                      +   G+           Y +WI TYSS  FETAA  +E LLD+ S      AAE+ S+ SLY +A+  E +FF AQ                            GS  F P  E +         ++ R  +L  DFD T T  D++ +L + A            V R+W  L+  +   ++++ EE +  +    A+     +    +++G   ++A  + LQ + +D + +   +      C S     S +   +      +  F             +  PV SN+L  D +  +TG +  +V     K +         RE      A      +++GDS+TDLLA+L AD+GIV+GTS     +  ++AFG+ I PL +   V  +   +     +EG       +YRASGW EI+ FL G+
Sbjct:   30 LWQQSKREAMVCLYHPFVVSIAAGTLDLHSFQRFIAQDSFFLTAFAKAYGLAIERSDDREVKSEICKLQQAVYEELELHSSLMKAWNFD-HTPPSPATCAYTDFLLAVAAGKKIECEKTKVPMLALAAMAPCMRLYAFLGQETRVFSRENHPYRDWISTYSSPGFETAATRLEQLLDSLSEAQETTAAEFQSMQSLYHRAIAYEVSFFDAQEVR-------------------------GSNAFVPLLESVA--------LKDRNFVLISDFDSTCTVSDSSPVLAELAMAV------DPNVRRKWSSLSDEYFRDYSKLLEEVVLREYDYDAIKEALQVLSEFEKQGNAKIDAS-RVLQGIKIDDIKQAGQNMALQAGCASVLCRLSSKISCQILSVCWSRTFIEAAFSKENI---TNVPVHSNELENDGN-FTTGSLIRRVETPIDKEETMF------REI---LHAPDDKFVIFIGDSLTDLLALLRADIGIVLGTSSS--LDRASKAFGVKIVPLFSGL-VQRQQSSRSAWRKEEG------VLYRASGWLEIEAFLAGN 530          

BLAST of mRNA_M-pyrifera_M_contig12773.5585.1 vs. uniprot
Match: A0A8J9SNJ0_9CHLO (Probable thiamine biosynthetic bifunctional enzyme TH1, chloroplastic at C-terminar half n=1 Tax=Coccomyxa sp. Obi TaxID=2315456 RepID=A0A8J9SNJ0_9CHLO)

HSP 1 Score: 149 bits (377), Expect = 1.120e-34
Identity = 163/578 (28.20%), Postives = 228/578 (39.45%), Query Frame = 1

Query:   22 LWKDCQLEAMRSLHHPWTLGLATGTTSRHSFQHYVAQDAYFLRAFVKAYAYALTKV---EDEGDVRAFHSLIGSVLDELGLHASYATKWGLDIHN-------------------------------------------ELKHAFPLWGESVYAEWIETYSSEEFETAAVLVETLLDAHSAAAEYPSLLSLYRKAMGLEYAFFAAQSQGTAEDDRPHHLTQGDDSSGSVQISSGGSVTFQPGEKIGKGRGVFSGVQHRVALLCVDFDDTLTDGDTTSLLVKTAKGQRGT---EDGQAEVDREWKV----LTSLFLNKWNEVTEESLAAKV--------RSTALLYGEDLSGAVDRKGLEAMLQK-----LQTVDLDSVSRVSDSKVRMTRCLSRALASSFQTHV-----KTRGTRLAYRFAAGELKLPGFGGDSPAPVTSNDLLYDEHGVSTGDIAIKVSGSFGKHQQFLNLAATARETARGFEAVPRTMSVYVGDSVTDLLAMLDADVGIVVGTSDGDAFEIVARAFGIGIRPLAAAYGVLGETGGQGGGSLDEGSTGRGSCIYRASGWPEIDVFLFG 1542
            LW D ++E +R+LHHP+   L  GT  R SFQ Y+AQDA+FL AF  AYA A  K    +D        +L   V  EL LH SYA +WG+D+ +                                           EL  A+P      Y +WI TYSS  +       E LLD      ++  L +LYR AM  E  FF A                                             + +    RV +L +DFD+TLT  D+TS+++ TA     T   E GQA V    +     L   ++ + +E+ E+ L A           S    + ++LS + DR+   A++       + T  L    R    +      L RA  +  QTHV          R A R   G              V +NDL     G+STGD+  ++     K      L   +    +G       +SVYVGDS++DL  +L  D+GIV+G +       VARA GI ++PL AA                EG +  G+ +Y A  WPEI+ FLFG
Sbjct:   11 LWADSEVEVLRALHHPFVHSLGNGTLQRESFQFYIAQDAHFLAAFSSAYAAAARKASAAKDTATAAQLQTLYEGVAKELELHGSYAEEWGVDLADMVTPSRATQAYVDFLSNIAETRGVAEILAAMTPCSRLYGYLGLELAAAYPQ-ASHAYNDWILTYSSPAYLALPEAKEALLDKLGRDCDFGVLRNLYRTAMRHELQFFDAA--------------------------------------------ISTHASPRVGVLVLDFDETLTVSDSTSVIINTAIDAAETAMREGGQASVREVLEAKRDDLVENYIRERDELLEDLLPAAEPANPPPFNMSFMAEFVDELS-SFDRRMNAAVIDSGILAHVPTNALAKQGRTIPFQKGAVSFLRRAKQAGVQTHVVSVSWSAEMVRGALRGRIGVAHSEETARAGQVVVHANDLECSL-GISTGDLHRRIECGVDKAAALDVLLLKSAAKRQG--PFGEKLSVYVGDSMSDLAPLLSVDLGIVIGQNK--LLRRVARAAGITLKPLVAAP--------------LEGKSEEGT-LYEARSWPEIEAFLFG 522          

BLAST of mRNA_M-pyrifera_M_contig12773.5585.1 vs. uniprot
Match: A0A8J5XNG5_DIALT (Thiamine phosphate synthase n=2 Tax=Diacronema lutheri TaxID=2081491 RepID=A0A8J5XNG5_DIALT)

HSP 1 Score: 148 bits (374), Expect = 2.930e-34
Identity = 157/556 (28.24%), Postives = 222/556 (39.93%), Query Frame = 1

Query:   22 LWKDCQLEAMRSLHHPWTLGLATGTTSRHSFQHYVAQDAYFLRAFVKAYAYALTKVEDEGDVRAFHSLIGSVLDELGLHASYATKWGLDIHN-------------------------------------------ELKHAFPLWGESVYAEWIETYSSEEFETAAVLVETLLDAHSAAAEYPSLLSLYRKAMGLEYAFFAAQSQGTAEDDRPHHLTQGDDSSGSVQISSGGSVTFQPGEKIGKGRGVFSGVQHRVALLCVDFDDTLTDGDTTSLLVKTAKGQRGTEDGQAEVDREWKVLTSLFLNKWNEVTEESLAAKVRSTALLYGE-------DLSGAVDRKGLEAMLQ--KLQTVDLDSVSRVSDSKVRMTRCLSRAL-----------ASSFQTHVKTRGTRLAYRFAAGELKLPGFGGDSPAPVTSNDLL-------------YDEHGVSTGDI--AIKVSGSFGKHQQFLNLAAT-ARETARGFEAVPRTMSVYVGDSVTDLLAMLDADVGIVVGTSDGDAFEIVARAFGIGIRPLAAAYGVLGETGGQG 1452
            LW      A RSLH P+ L LA GT  R SFQ YVAQDA+FLRAF +AYA AL++ E +G +    SLI  V+DEL LH  YA KW LD+                                              L  AFP      YA+W+ TYSS+EF   A  +E LLD  +      +  + Y +AM LE  FFA+Q  G A  D                          P +++               L  VDFD+T TD  T  LL + A    G  D +A    EW+ L+  F  ++    E  +       A   GE       +   A +R+ +EA  +   L+ V   ++++    +V++    +R             A     +   +  R A R      +  G G    A V + +L+             +D    +TG++  A  +  S  K   F  L A  AR  A G       +++Y GDS+TDL  +L A +GIV+G S          + G+ ++PL AA   L E  G G
Sbjct:    9 LWASALPLATRSLHSPFVLRLAAGTLPRASFQGYVAQDAFFLRAFARAYALALSRCESDGAIGELSSLIAGVVDELALHHGYAAKWDLDLAGVRPTAATSAYTEFLLALARSDAPIAVVLAAMTPCMRLYAFLGQRLCAAFPD-ASHAYADWLRTYSSDEFGALAGRIEALLDECADGVPLDAARAAYTRAMQLELDFFASQP-GVAPVDG----------------------LLAPADRL---------------LFAVDFDETATDAQTLPLLAQLAAEHAGAADERAARVAEWRTLSETFAREYTMAIERIVPPXXXXXAPPAGEAAVVEMVERLSAFERRAVEAAERGRALRGVPEAALAKAGAERVKLRDGFARLAYALRAAGACERAEVISVNWAAQLARAALRARLDAARPVGAG----ATVGAEELVRLHCNEMPVAIDAHDGAPRTTGELRAAAGLHTSADKGAAFGQLCAEYARAHAGG---AGELLTLYAGDSITDLQPLLAARIGIVLGHSSS--LRAALSSLGVRVQPLVAARRALAECAGAG 516          

BLAST of mRNA_M-pyrifera_M_contig12773.5585.1 vs. uniprot
Match: A0A836B2K3_CHLIN (Thiamine phosphate synthase n=1 Tax=Chlamydomonas incerta TaxID=51695 RepID=A0A836B2K3_CHLIN)

HSP 1 Score: 147 bits (370), Expect = 9.520e-34
Identity = 172/613 (28.06%), Postives = 226/613 (36.87%), Query Frame = 1

Query:   22 LWKDCQLEAMRSLHHPWTLGLATGTTSRHSFQHYVAQDAYFLRAFVKAYAYALTKVE--DEGDVRAFHSLIGSVLDELGLHASYATKWGLD-------------------------------IHNELKHAFP---LWG--------------------ESVYAEWIETYSSEEFETAAVLVETLLDAHSAA-AEYPSLLSLYRKAMGLEYAFFAAQSQGTAEDDRPHHLTQGDDSSGSVQISSGGSVTFQPGEKIGKGRGVFSGVQHRVALLCVDFDDTLTDGDTTSLLVKTAKGQRGTEDGQAEVDREWKVLTSLFLNKWNEVTEESLAAKVRSTALLYGEDLSGAVDRKGLEAMLQKLQTVDLD--------SVSRVSDS------------KVRMTRCLSRALASSFQTHVKTR-------GTRLAYRFAAG-------------ELKLPGFGGDSPA-PVTSNDLLYDEHGVSTGDIAIKVSGSFGKHQQFLNL--------AATARETARGFEAVPRTMSVYVGDSVTDLLAMLDADVGIVVGTSDGDAFEIVARAFGIGIRPLAAAYGVLGETGGQGGGSLDEGSTGRGSCIYRASGWPEIDVFLFG 1542
            LW + Q E   SLH P+  G+A GT  + +FQHYVAQDAYFL  F +AY  AL+K    D+        L+  V +EL LH SYA KWG+D                               +   L    P   L+G                       Y EW+ TYSS ++ +   L E + +  +   A+   LL LYR+AM LE  FFAAQ                                             +S    R + L VDFD+T T  DT   L++ A    G +      D  W   TS  L       +  L  ++    L  G   + A D  GL+A L  L   D           + R S S            + +    LS AL      HV +        G  LA    A               ++L G  G  PA  +  N L  D  G++TG +A +V  +  K      L        AA A + A    +      VYVGDS +DL AMLD+D G+V+G++       V R FGI I PLAAA              LD      G+      GW EI  FLFG
Sbjct:   19 LWDNVQHEVFMSLHDPFVQGMAQGTLDKRAFQHYVAQDAYFLHYFARAYGIALSKALALDDSTFEDLSGLLRGVHEELQLHGSYAAKWGVDMTVAGTRPSAATKAYTDFLMNVADAEGQDGSVAEILAAMLPCARLYGFLGAALAGAHGPQAPDQAPPAHHYWEWVRTYSSADYLSVPGLKERMFERLAGKEADKAKLLRLYRRAMQLEAEFFAAQP--------------------------------------------YSPPPRRFSALVVDFDETCTAKDTIGGLMRLAAAAAGQQGRATAGDAAWVAATSSRLAADYVAAQGELLGRL----LPEGAPDAAAYDAAGLDAFLAALSDFDAAMNAPVEACGILRGSTSAEVAAAGAGLQLRPQCREVLSGALGRGIPVHVLSVNWSDVYVGAALAAPATAAVXXXXXXXXXSALPVRLAGAEGAGPAVELRCNSLELDGAGLTTGALAKRVQSARDKRAALAELRSRLPAAPAAAAVDGASDSGSDNPAALVYVGDSTSDLGAMLDSDFGVVIGSNA--LLRRVCRRFGIRILPLAAA-------------PLDPRRVPAGTLYEAVGGWAEIRAFLFG 568          

BLAST of mRNA_M-pyrifera_M_contig12773.5585.1 vs. uniprot
Match: A0A150GK88_GONPE (Thiamine phosphate synthase n=1 Tax=Gonium pectorale TaxID=33097 RepID=A0A150GK88_GONPE)

HSP 1 Score: 143 bits (360), Expect = 1.830e-32
Identity = 172/622 (27.65%), Postives = 236/622 (37.94%), Query Frame = 1

Query:   22 LWKDCQLEAMRSLHHPWTLGLATGTTSRHSFQHYVAQDAYFLRAFVKAYAYALTKVE--DEGDVRAFHSLIGSVLDELGLHASYATKWGLDI-----------------------------------------HNE-------------LKHAFPLWGESVYA--------------------EWIETYSSEEFETAAVLVETLLDAHSAAAEYPSLLSLYRKAMGLEYAFFAAQSQGTAEDDRPHHLTQGDDSSGSVQISSGGSVTFQPGEKIGKGRGVFSGVQHRVALLCVDFDDTLTDGDTTSLLV--KTAKGQRGTEDGQAEVDREWKVLTSLFLNKWNEVTEESLAAKVRSTALLYGEDL--SGAVDRKGLEAMLQKLQTVDLDSVSRVSDSKV--------------------RMTRCLSRALASSFQTHVKTRGTRLAYRFAAGEL--------KLPGFGGDSPAP----VTSNDLLYDEHGVSTGDIAIKVSGSFGKHQQFLNLAATARETARGFEAVPRTMS---VYVGDSVTDLLAMLDADVGIVVGTSDGDAFEIVARAFGIGIRPLAAAYGVLGETGGQGGGSLDEGSTGRGSCIYRASGWPEIDVFLFG 1542
            LW+  Q E   SLH P+  G+A GT  R +FQHYVAQDA+FL+ F +AY  AL+K    D+        L+  V +EL LH SYA +WG+++                                         H E                 +   G ++ A                    EW++TYSS ++     L E + D  +A ++   L SLYR+AM LE  FFAAQ         PH                                   S    R+A L VDFD+T T  DT   L+    A  + G   G    D  W   T         + +  +AA+ +  A L  E    S   D  GL + L++L   D    + V +S +                         LS AL       V +      Y  AA  L        + P  G  + A     ++ N L  D  G+++G +  +V  +  K     N  A  RE                VYVGDSV+DL A+L ADVG+VVGT+       VA  FG+ +RPLAAA   L  T  +   S    + G+G  +Y A+GW EI  FLFG
Sbjct:   21 LWESSQHEVFMSLHDPFVQGMAQGTLDRRAFQHYVAQDAHFLKYFARAYGIALSKALALDDATFATLSRLLRGVHEELRLHVSYAARWGVELGLEEEGEAVGSXXXXXXXXGRGVASSATRAYTDFLMSVAEAHGEDGGVAEILAAMLPCSRLYGYLGSALAAAHGAGGAGSGLGPSPANEFWEWVQTYSSPDYLAIPALKEAMFDRLAAHSDKGRLQSLYRRAMQLEAEFFAAQ---------PH-----------------------------------SPPPRRIAALVVDFDETCTPRDTIGGLMGLAAAAARAGRPTGG---DASWAEATP------GRLADNYVAAQGQLLAELLPEGAAPSPEYDPAGLSSFLERLSDFDERMNAVVEESGILKGSTEQELAAAGAALPLRPHCRETLSGALRRGIPVRVLSVNWSDVYVGAALGLPTQLARADEAPAMGAGAAAGGAVGLSCNSLQLDSAGLTSGRLTKRVQSARDKR----NALAALREALAAXXXXXXXXXXAVVYVGDSVSDLAALLAADVGVVVGTNR--LLRRVAERFGVRLRPLAAAP--LAATAAEPP-STSSAAGGKG-VLYEAAGWEEIRAFLFG 579          

BLAST of mRNA_M-pyrifera_M_contig12773.5585.1 vs. uniprot
Match: A0A383WBI9_TETOB (Thiamine phosphate synthase n=2 Tax=Tetradesmus obliquus TaxID=3088 RepID=A0A383WBI9_TETOB)

HSP 1 Score: 143 bits (360), Expect = 1.950e-32
Identity = 156/616 (25.32%), Postives = 231/616 (37.50%), Query Frame = 1

Query:   22 LWKDCQLEAMRSLHHPWTLGLATGTTSRHSFQHYVAQDAYFLRAFVKAYAYALTKVEDEGDVRAFHSLIGSVL----DELGLHASYATKWGL----------------------------DIHNELKHAFP---LWG--------------ESVYAEWIETYSSEEFETAAVLVETLLDAHSAAAEYPSLLSLYRKAMGLEYAFFAAQSQGTAEDDRPHHLTQGDDSSGSVQISSGGSVTFQPGEKIGKGRGVFSGVQHRVALLCVDFDDTLTDGDTTSLLVKTA------------KGQRGTEDGQAEVDREWKVLTSLFLNKWNEVTEESLAAKVRSTALLYGEDLS---GAVDRKGLEAMLQKLQTVDLDSVSRVSDSKVRMTRCLSRALASSFQTHVKTRGTRLAYRFAAGELKLPGFGGD----------SPAP----------------------------VTSNDLLYDEHGVSTGDIAIKVSGSFGKHQQFLNLAATARETARGFEAVPRTMS---VYVGDSVTDLLAMLDADVGIVVGTSDGDAFEIVARAFGIGIRPLAAAYGVLGETGGQGGGSLDEGSTGRGSCIYRASGWPEIDVFLFGDGHH 1554
            LW D Q EAM +LHHP+   LA GT  R  FQHYV QD +FL  F +AYA A+ K      + A  +++  +L     EL LH SYA KWG+                             +   L    P   L+G                 Y+EW+ TYS  E+  + V+ E L D  +AA  Y  L  LY +AM LE  FF+AQ  G A    P H                                        V LL  DFDDT+T+ DT   L+  A                     + +++ + K L   ++ K  ++    L A   ++A   G   S    + D+ GL A  + L   D++    V D+        +  +A++    ++    R     AA  L +     +           PA                             + +N+L++   GVSTGD+  ++  +  K +    L    R ++              VYVGDS +D+L +L AD GI++G++          AFG+G++PL +A   +  +               G  +Y AS W EI  FLFG   H
Sbjct:   44 LWADSQAEAMEALHHPFVQALAAGTLDRACFQHYVLQDLFFLTRFARAYAAAIDKCGTTPALAAAKAVLAELLLGVDAELKLHGSYAAKWGVAVDEWVWPSAATSQYTDFLMQVATDPETSVAEVLAAMIPCARLYGFLGCQLAAATQGLPRHAYSEWVATYSGAEYLASPVMKEELFDKIAAAEPYDRLKQLYTRAMQLEVNFFSAQPNGPA----PRH----------------------------------------VGLLVCDFDDTITEKDTIGTLLGAAVEXXXXXXXXXXXXXXXXXXXRQQLNTQVKNLADNYMTKQQQLLASLLPADDDASAAAAGVSNSVTNKSYDKDGLLAFCEALSDFDVEMNQVVVDAGCLAGLTKAGVIAAASNITLRPGSARTLSAAAAAGLPVALLSVNWSKSLMQAVLQPAAXXXXXXXXXXXXXXXXXXXXXXXXXXMEIHANELVWGPDGVSTGDLQREIQCARDKGRVMDTLLQQHRSSSXXXXXXXXXXXXAVVYVGDSSSDILPLLKADYGIIIGSNK--LLRKALAAFGVGLQPLCSAP--MTPSSXXXXXXXXXXXXXEGPVLYEASSWQEIAAFLFGPTWH 611