mRNA_M-pyrifera_M_contig1277.5578.1 (mRNA) Macrocystis pyrifera P11B4 male

BLAST of mRNA_M-pyrifera_M_contig1277.5578.1 vs. uniprot
Match: D7FVE5_ECTSI (Glutamine-dependent NAD(+) synthetase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FVE5_ECTSI)

HSP 1 Score: 1176 bits (3043), Expect = 0.000e+0
Identity = 586/659 (88.92%), Postives = 620/659 (94.08%), Query Frame = 1

Query:    4 CDATDDILCDIGMPVQHLGVRYNCRVFCLNRKILAIRPKLHLADDGNYRETRWFTTWKRRNEMEDHTLCRGLAEVTGQDKVPFGQAAISALDALIAGETCEEIWTPDSPHIGQTLAGVEIIGNGSGSHHQLRKLDTRLSYMISATAKCGGVYVYSNQRGCDGGRLYYDGCALIVVNGNVVAQAAQFSLEDVEVITATVNLEDVRSYRASVSSRMEQASDARRLPTVEAPLFCLGTPDANYITHPPTQPQALRMHSPEEECALGPACWLWDYLRRSGSAGFFLPLSGGADSSSVAAIVGVMCGLAVDAAAAEKAELVAAAANGE---EGGKAGEGTVTREVRRLMGLNEEQEVPTDPRELASCVLHTCFMGTDNSSSATRGRAATLADQIGAYHNTITIDTAVSAVVGVFCALTGKTPQFLSRGGSSAEDLALQNIQARLRMVMAYLLAQLLPWVRGRQGFLLVLGSANVDEALRGYMTKYDCSSADLNPIGGISKVDLKRLLAWAARERDYPALAEIAAAPPTAELRPLAEGADEADEHSQVDEEDMGMTYEELSHFGRLRKVARCGPVSMFQNLLSAWRHLSPAEIAAKVKRFFFFYSVNRHKMTTLTPSYHAEEYSPDDNRFDLRPFLYPTRWPRQFAAIDSMLKGLPPGRIDGGQA 1971
            CDATD ILCDIGMPVQHLGVRYNCRVFCLNRKILAIRPKLHLADDGNYRETRWFTTWKRRNE EDHTLCRGLAEVTGQDKVPFGQ A+SALDAL+AGETCEE+WTPDSPHIGQ LAGV+IIGNGSGSHHQLRKLDTRL+YMISATAKCGGVYVYSNQRGCDGGRLYYDGCALIVVNG+VVAQAAQFSL DVEVITATVNLEDVRSYRASVSSRMEQAS ARRLPTVEAP FCLGTP ANY+THPPT PQAL++HSP+EECALGPACWLWDYLRRSGSAGFFLPLSGGADSSSVAAIVGVMCGLAV+ AAAE AEL     + E   + G AG G+V++EVRRLMGL E ++VP+ PR+LA+CVLHTCFMGT+NSS+ATR RA+TLADQIGAYH+ I IDTAV+A+VGVF  LTGKTP+FLSRGG+SAEDLALQNIQARLRMVMAYLLAQLLPWVRGRQGFLLVLGSANVDEALRGYMTKYDCSSADLNPIGGISKVDLK+LL WAARER Y ALA+IAAAPPTAELRPLAEGA EADEHSQVDEEDMGMTYEELSHFGRLRKVARCGPVSMFQNLLSAWRHLSP EIA+KVKRFFFFYSVNRHKMTTLTPSYHAEEYSPDDNRFDLRPFLYPTRWPRQFA IDSMLKGLP GR+DG +A
Sbjct:   83 CDATDGILCDIGMPVQHLGVRYNCRVFCLNRKILAIRPKLHLADDGNYRETRWFTTWKRRNETEDHTLCRGLAEVTGQDKVPFGQTAVSALDALVAGETCEELWTPDSPHIGQALAGVDIIGNGSGSHHQLRKLDTRLNYMISATAKCGGVYVYSNQRGCDGGRLYYDGCALIVVNGDVVAQAAQFSLADVEVITATVNLEDVRSYRASVSSRMEQASGARRLPTVEAPSFCLGTPGANYVTHPPTLPQALKIHSPQEECALGPACWLWDYLRRSGSAGFFLPLSGGADSSSVAAIVGVMCGLAVETAAAENAELSGIDDDAERKSKEGAAGVGSVSKEVRRLMGLKEGEKVPSSPRDLANCVLHTCFMGTENSSNATRARASTLADQIGAYHSNIVIDTAVAALVGVFRTLTGKTPRFLSRGGTSAEDLALQNIQARLRMVMAYLLAQLLPWVRGRQGFLLVLGSANVDEALRGYMTKYDCSSADLNPIGGISKVDLKKLLEWAARERGYTALADIAAAPPTAELRPLAEGAGEADEHSQVDEEDMGMTYEELSHFGRLRKVARCGPVSMFQNLLSAWRHLSPQEIASKVKRFFFFYSVNRHKMTTLTPSYHAEEYSPDDNRFDLRPFLYPTRWPRQFAVIDSMLKGLPSGRVDGKEA 741          

BLAST of mRNA_M-pyrifera_M_contig1277.5578.1 vs. uniprot
Match: A0A6H5JI49_9PHAE (NAD(+) synthase (glutamine-hydrolyzing) n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JI49_9PHAE)

HSP 1 Score: 1170 bits (3027), Expect = 0.000e+0
Identity = 583/659 (88.47%), Postives = 615/659 (93.32%), Query Frame = 1

Query:    4 CDATDDILCDIGMPVQHLGVRYNCRVFCLNRKILAIRPKLHLADDGNYRETRWFTTWKRRNEMEDHTLCRGLAEVTGQDKVPFGQAAISALDALIAGETCEEIWTPDSPHIGQTLAGVEIIGNGSGSHHQLRKLDTRLSYMISATAKCGGVYVYSNQRGCDGGRLYYDGCALIVVNGNVVAQAAQFSLEDVEVITATVNLEDVRSYRASVSSRMEQASDARRLPTVEAPLFCLGTPDANYITHPPTQPQALRMHSPEEECALGPACWLWDYLRRSGSAGFFLPLSGGADSSSVAAIVGVMCGLAVDAAAAEKAELVAA---AANGEEGGKAGEGTVTREVRRLMGLNEEQEVPTDPRELASCVLHTCFMGTDNSSSATRGRAATLADQIGAYHNTITIDTAVSAVVGVFCALTGKTPQFLSRGGSSAEDLALQNIQARLRMVMAYLLAQLLPWVRGRQGFLLVLGSANVDEALRGYMTKYDCSSADLNPIGGISKVDLKRLLAWAARERDYPALAEIAAAPPTAELRPLAEGADEADEHSQVDEEDMGMTYEELSHFGRLRKVARCGPVSMFQNLLSAWRHLSPAEIAAKVKRFFFFYSVNRHKMTTLTPSYHAEEYSPDDNRFDLRPFLYPTRWPRQFAAIDSMLKGLPPGRIDGGQA 1971
            CDATD ILCDIGMPVQHLGVRYNCRVFCLNRKILAIRPKLHLADDGNYRETRWFTTWKRRNE EDHTLCRGLAEVTGQDKVPFGQ A+SALDAL+AGETCEE+WTPDSPHIGQ LAGV+IIGNGSGSHHQLRKLDTRL+YMISATAKCGGVYVYSNQRGCDGGRLYYDGCALIVVNG+VVAQAAQFSL DVEVITATVNLEDVRSYRASVSSRMEQAS ARRLPTVEAP FCLGTP ANY+THPPT PQAL++HSP+EEC LGPACWLWDYLRRSGSAGFFLPLSGGADSSSVAAIVGVMCGLA++ AAAEKAEL      A    + G AG G+V+ EVRRLMGL E ++VP+ PR+LA+CVLHTCFMGT+NSS+ATR RA+ LADQIGAYH+ I IDTAV+AVVGVF  LTGKTP+FLSRGG+SAEDLALQNIQARLRMVMAYLLAQLLPWVRGRQGFLLVLGSANVDEALRGYMTKYDCSSADLNPIGGISKVDLK+LL W ARER Y ALA+IAAAPPTAELRPLAEGA EADEHSQVDEEDMGMTYEELSHFGRLRKVARCGPVSMFQNLLSAWRHLSP EIA+KVKRFF FYSVNRHKMTTLTPSYHAEEYSPDDNRFDLRPFLYPTRWPRQFA IDSMLKGLP GR+DG +A
Sbjct:  157 CDATDGILCDIGMPVQHLGVRYNCRVFCLNRKILAIRPKLHLADDGNYRETRWFTTWKRRNETEDHTLCRGLAEVTGQDKVPFGQTAVSALDALVAGETCEELWTPDSPHIGQALAGVDIIGNGSGSHHQLRKLDTRLNYMISATAKCGGVYVYSNQRGCDGGRLYYDGCALIVVNGDVVAQAAQFSLADVEVITATVNLEDVRSYRASVSSRMEQASGARRLPTVEAPSFCLGTPGANYVTHPPTLPQALKIHSPQEECVLGPACWLWDYLRRSGSAGFFLPLSGGADSSSVAAIVGVMCGLAIETAAAEKAELSGMDDDAKRKSKEGVAGVGSVSNEVRRLMGLKEGEKVPSSPRDLANCVLHTCFMGTENSSNATRARASALADQIGAYHSNIVIDTAVAAVVGVFRTLTGKTPRFLSRGGTSAEDLALQNIQARLRMVMAYLLAQLLPWVRGRQGFLLVLGSANVDEALRGYMTKYDCSSADLNPIGGISKVDLKKLLEWVARERGYTALADIAAAPPTAELRPLAEGAGEADEHSQVDEEDMGMTYEELSHFGRLRKVARCGPVSMFQNLLSAWRHLSPQEIASKVKRFFIFYSVNRHKMTTLTPSYHAEEYSPDDNRFDLRPFLYPTRWPRQFAVIDSMLKGLPSGRVDGKEA 815          

BLAST of mRNA_M-pyrifera_M_contig1277.5578.1 vs. uniprot
Match: A0A835ZEW9_9STRA (Glutamine-dependent NAD(+) synthetase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZEW9_9STRA)

HSP 1 Score: 854 bits (2206), Expect = 1.480e-303
Identity = 433/674 (64.24%), Postives = 509/674 (75.52%), Query Frame = 1

Query:    7 DATDDILCDIGMPVQHLGVRYNCRVFCLNRKILAIRPKLHLADDGNYRETRWFTTWKRRNEMEDHTLCRGLAEVTGQDKVPFGQAAISALDALIAGETCEEIWTPDSPHIGQTLAGVEIIGNGSGSHHQLRKLDTRLSYMISATAKCGGVYVYSNQRGCDGGRLYYDGCALIVVNGNVVAQAAQFSLEDVEVITATVNLEDVRSYRASVSSRMEQASDARRLPT--------------------------VEAPLFCLGTPDANYITHPPTQPQALRMHSPEEECALGPACWLWDYLRRSGSAGFFLPLSGGADSSSVAAIVGVMCGLAVDAAAAEKAELVAAAANGEEGGKAGEGTVTREVRRLMGLNEEQEVPTDPRELASCVLHTCFMGTDNSSSATRGRAATLADQIGAYHNTITIDTAVSAVVGVFCALTGKTPQFLSRGGSSAEDLALQNIQARLRMVMAYLLAQLLPWVRGRQGFLLVLGSANVDEALRGYMTKYDCSSADLNPIGGISKVDLKRLLAWAARERDYPALAEIAAAPPTAELRPLAEGADEADEHSQVDEEDMGMTYEELSHFGRLRKVARCGPVSMFQNLLSAWRHLSPAEIAAKVKRFFFFYSVNRHKMTTLTPSYHAEEYSPDDNRFDLRPFLYPTRWPRQFAAIDSMLKGLPPG 1950
            DAT  ILCDIG+PV H GVRYNCR FCL+ KIL +RPKL +ADDGNYRE+R FT+WKR NE+EDH L   L + TGQ  VPFG  A++A D ++A ETCEE+WTP SPHI Q+LAGVEI GNGS SHH+LRKLDTR+SYM++AT KCGGVY+Y+NQ+GCDGGRLY+DGCALIV NG ++AQ +QFS  DVEV+TATVNL+DVR+YRA+ +SR +Q+S                                  +   F L T  A Y THPPT P  L++H+PEEECALGPACWLWDYLRRS +AGFFLPLSGGADSSS A IVGVMC L V+AA                  + G+  V  +VRR+ G++    +P+  + LA+ ++HT +MGT NS +ATR RAA LA QIGA H+ + ID AV+AVVGVF ALTG TP+F SRGGS  EDLALQNIQARLRMV AYLLAQLLPW RGR GFLLVLGSANVDE+LRGYMTKYDCSSADLNPIG ISKVDLKR+L+WAA    + +L EI AAPPTAELRP+ EGAD+  EHSQ+DE+DMGMTYEELSHFGRLRKV  CGPVSMF++L++ W  L+P+ +AAKVK FF+FY VNRHKMTTLTP+YHAE YSPDDNRFDLRPFLY  RWPRQF  ID+M   +  G
Sbjct:   95 DATYGILCDIGLPVMHAGVRYNCRAFCLDGKILLLRPKLFMADDGNYRESRHFTSWKRANEVEDHVLSIALQDATGQFSVPFGHGAVAATDTVVATETCEELWTPRSPHIEQSLAGVEITGNGSASHHELRKLDTRVSYMLAATRKCGGVYLYANQQGCDGGRLYFDGCALIVCNGKLLAQGSQFSAADVEVVTATVNLDDVRAYRAACASRSDQSSRGXXXXXXLIRAXXXXXXXXXXXXXXAGALGGGAQPQSFRLATDGATYATHPPTVPTTLKLHTPEEECALGPACWLWDYLRRSRAAGFFLPLSGGADSSSTAVIVGVMCHLVVEAA------------------ERGDRQVASDVRRIAGMSSGDPLPS-AQALANSLMHTAYMGTANSGAATRRRAAALAGQIGACHSALDIDAAVAAVVGVFRALTGNTPRFASRGGSETEDLALQNIQARLRMVFAYLLAQLLPWARGRPGFLLVLGSANVDESLRGYMTKYDCSSADLNPIGAISKVDLKRMLSWAAERYGWDSLTEIGAAPPTAELRPMEEGADDGGEHSQLDEDDMGMTYEELSHFGRLRKVGLCGPVSMFRSLVALWPALAPSAVAAKVKLFFYFYGVNRHKMTTLTPAYHAEAYSPDDNRFDLRPFLYNMRWPRQFRDIDAMAGDMEAG 749          

BLAST of mRNA_M-pyrifera_M_contig1277.5578.1 vs. uniprot
Match: A0A485LD09_9STRA (Glutamine-dependent NAD(+) synthetase n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485LD09_9STRA)

HSP 1 Score: 822 bits (2123), Expect = 8.860e-292
Identity = 430/653 (65.85%), Postives = 504/653 (77.18%), Query Frame = 1

Query:    7 DATDDILCDIGMPVQHLGVRYNCRVFCLNRKILAIRPKLHLADDGNYRETRWFTTWKRRNE----MEDHTLCRGLAEVTGQDKVPFGQAAISALDALIAGETCEEIWTPDSPHIGQTLAGVEIIGNGSGSHHQLRKLDTRLSYMISATAKCGGVYVYSNQRGCDGGRLYYDGCALIVVNGNVVAQAAQFSLEDVEVITATVNLEDVRSYRASVSSRMEQAS--DARRLPTVEAPLFCLGTPDANYITHPPTQPQALRMHSPEEECALGPACWLWDYLRRSGSAGFFLPLSGGADSSSVAAIVGVMCGLAVDAAAAEKAELVAAAANGEEGGKAGEGTVTREVRRLMGLNEEQE-VPTDPRELASCVLHTCFMGTDNSSSATRGRAATLADQIGAYHNTITIDTAVSAVVGVFCALTGKTPQFLSRGGSSAEDLALQNIQARLRMVMAYLLAQLLPWVRGRQGFLLVLGSANVDEALRGYMTKYDCSSADLNPIGGISKVDLKRLLAWAARERDYPALAEIAAAPPTAELRPLA-EGADEADEHSQVDEEDMGMTYEELSHFGRLRKVARCGPVSMFQNLLSAWRHLSPAEIAAKVKRFFFFYSVNRHKMTTLTPSYHAEEYSPDDNRFDLRPFLYPTRWPRQFAAIDSMLKGL 1941
            D TDDILCDIGMPV H GVRYNCRVFCLNRKI+ IRPKL+LADDGNYRE RWFTTWK   +    +  H L   + ++TGQ  VPFG  A+ ALD++I+ ETCEE++TPD+PHIG +L GVEIIGNGSGSHH+LRKL  R+  +  ATAK GG+Y+YSNQ+GCDGGRLYYDGCALIVVNG++VAQ  QFS+ DVEVITA V+L+DVRSYR +VSSR EQAS  + R L  V+ P F +   +A ++   P  P  +++H+PEEE ALGPACWLWDYLRRS + GFFLPLSGGADS+SVAAIVGVMC LA  AA            NG      G+  V R+++ L+G +E    VP  P +LA+ VLHT +MGT+NSS AT+ RA  LA +IG+YH  +TIDT VSAVV  F  L GKTP+F   GG+ +EDLALQNIQARLRMVMAYLLAQLLPWVR R GFLLVLGSANVDEALRGYMTKYDCSSADLNPIG +SK DLKRLL +A+ +  YP LAEI AAPPTAELRP + EG     EH+QVDEEDMGMTY+ELS FGRLRK+ RCGP+ MF+ L + W HLSP EIAAKVKRFFF+Y +NRHKMTTLTPSYHAE YSPDDNRFDLRPFLY ++W RQF +ID +   +
Sbjct:   78 DVTDDILCDIGMPVMHQGVRYNCRVFCLNRKIVFIRPKLYLADDGNYREKRWFTTWKIHADHSLNLTTHMLPPSIQQLTGQINVPFGIGAVRALDSVISSETCEELFTPDAPHIGLSLGGVEIIGNGSGSHHELRKLQHRIDLIRGATAKAGGIYLYSNQQGCDGGRLYYDGCALIVVNGHIVAQGTQFSVADVEVITAVVDLDDVRSYRGAVSSRSEQASAPEQRSLARVDVP-FRMCQSEAGFLLPTPRIP--VKIHTPEEEIALGPACWLWDYLRRSKAKGFFLPLSGGADSASVAAIVGVMCHLATTAA------------NG------GDAQVQRDIQYLLGKDESAAYVPMRPSDLANSVLHTTYMGTENSSVATKSRATHLAAEIGSYHLNMTIDTMVSAVVSTFSLLVGKTPKFGCHGGTPSEDLALQNIQARLRMVMAYLLAQLLPWVRSRSGFLLVLGSANVDEALRGYMTKYDCSSADLNPIGAVSKTDLKRLLVYASTQYKYPTLAEIVAAPPTAELRPTSGEG-----EHTQVDEEDMGMTYDELSWFGRLRKIDRCGPLWMFRKLGAVWSHLSPLEIAAKVKRFFFYYGINRHKMTTLTPSYHAENYSPDDNRFDLRPFLYNSKWTRQFESIDILAAAM 704          

BLAST of mRNA_M-pyrifera_M_contig1277.5578.1 vs. uniprot
Match: K3WJV2_GLOUD (Glutamine-dependent NAD(+) synthetase n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3WJV2_GLOUD)

HSP 1 Score: 818 bits (2112), Expect = 3.720e-290
Identity = 422/649 (65.02%), Postives = 493/649 (75.96%), Query Frame = 1

Query:    7 DATDDILCDIGMPVQHLGVRYNCRVFCLNRKILAIRPKLHLADDGNYRETRWFTTWK--RRNEMEDHTLCRGLAEVTGQDKVPFGQAAISALDALIAGETCEEIWTPDSPHIGQTLAGVEIIGNGSGSHHQLRKLDTRLSYMISATAKCGGVYVYSNQRGCDGGRLYYDGCALIVVNGNVVAQAAQFSLEDVEVITATVNLEDVRSYRASVSSRMEQASDAR-RLPTVEAPLFCLGTPDANYI-THPPTQPQALRMHSPEEECALGPACWLWDYLRRSGSAGFFLPLSGGADSSSVAAIVGVMCGLAVDAAAAEKAELVAAAANGEEGGKAGEGTVTREVRRLMGLNEEQEVPTDPRELASCVLHTCFMGTDNSSSATRGRAATLADQIGAYHNTITIDTAVSAVVGVFCALTGKTPQFLSRGGSSAEDLALQNIQARLRMVMAYLLAQLLPWVRGRQGFLLVLGSANVDEALRGYMTKYDCSSADLNPIGGISKVDLKRLLAWAARERDYPALAEIAAAPPTAELRPLAEGADEADEHSQVDEEDMGMTYEELSHFGRLRKVARCGPVSMFQNLLSAWRHLSPAEIAAKVKRFFFFYSVNRHKMTTLTPSYHAEEYSPDDNRFDLRPFLYPTRWPRQFAAIDSMLKGL 1941
            D T+DILCDIGMPV H GVRYNCRV+CLN+KIL IRPKL  ADDGNYRETRWFTTWK   R  +E+H L   + ++TGQ  VPFG  AIS LD   A ETCEE++TPDSPHI  +LAGVEIIGNGSGSHHQLRKLD R+  +  AT K GGVY+Y+NQ+GCDGGR+YYDGCA+IVVNG VVAQ +QFS+ D+EV+TATV+L+DVRSYR S SSR EQAS     +P ++   F L   D+ ++  HPP +   +R H PEEE ALGPACW+WDYLRRSG +GFFLPLSGGADSSSVA IVG MC L VDAA            NG      G+  V ++V+R++G   E+  P   +ELAS +LHT +MGT NSS+AT+ RA +LA +IG YH  + +D  V AVV  F  LTGK+P+FLS+GG+  +DLALQNIQARLRMVMAYLLAQLLPWVR + GFLLVL SANVDEALRGYMTKYDCSS DLNPIG +SK DLK+LL W A   +YP L  +  APPTAELRP AE ADE  +HSQ+DEEDMGM+YEEL  FGRLRK+ RCGP  MF+ L   W HL+P  +A KVKRFFF+YSVNRHKMTTLTPSYHAE YSPDDNRFDLRPFLY TRW RQF +ID++   L
Sbjct:   77 DVTNDILCDIGMPVMHNGVRYNCRVYCLNQKILFIRPKLFHADDGNYRETRWFTTWKPSARKGLENHMLPPSIQKITGQVHVPFGAGAISTLDTCCASETCEELFTPDSPHINLSLAGVEIIGNGSGSHHQLRKLDQRMDLIRGATTKSGGVYLYANQQGCDGGRVYYDGCAVIVVNGQVVAQGSQFSVSDIEVVTATVDLDDVRSYRGSFSSRSEQASALDVNIPKIDVN-FSLCHEDSTFLYPHPPIE---MRYHVPEEEIALGPACWMWDYLRRSGGSGFFLPLSGGADSSSVACIVGAMCNLVVDAA------------NG------GDKRVIQDVQRIIGTTGEEYNPLTAQELASHILHTTYMGTKNSSAATKNRATSLAQEIGCYHLNMGMDMMVEAVVKTFEVLTGKSPRFLSQGGNIQQDLALQNIQARLRMVMAYLLAQLLPWVRSKNGFLLVLSSANVDEALRGYMTKYDCSSGDLNPIGAVSKGDLKKLLRWGAVRYNYPTLRTVVEAPPTAELRPTAENADEDADHSQLDEEDMGMSYEELGWFGRLRKIDRCGPYGMFRKLTHLWDHLTPTVVAEKVKRFFFYYSVNRHKMTTLTPSYHAENYSPDDNRFDLRPFLYNTRWTRQFHSIDALATKL 703          

BLAST of mRNA_M-pyrifera_M_contig1277.5578.1 vs. uniprot
Match: A0A6G0XTQ1_9STRA (Glutamine-dependent NAD(+) synthetase n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0XTQ1_9STRA)

HSP 1 Score: 817 bits (2111), Expect = 6.090e-290
Identity = 428/654 (65.44%), Postives = 498/654 (76.15%), Query Frame = 1

Query:    1 QCDATDDILCDIGMPVQHLGVRYNCRVFCLNRKILAIRPKLHLADDGNYRETRWFTTWKRRNE----MEDHTLCRGLAEVTGQDKVPFGQAAISALDALIAGETCEEIWTPDSPHIGQTLAGVEIIGNGSGSHHQLRKLDTRLSYMISATAKCGGVYVYSNQRGCDGGRLYYDGCALIVVNGNVVAQAAQFSLEDVEVITATVNLEDVRSYRASVSSRMEQAS--DARRLPTVEAPL-FCLGTPDANYITHPPTQPQALRMHSPEEECALGPACWLWDYLRRSGSAGFFLPLSGGADSSSVAAIVGVMCGLAVDAAAAEKAELVAAAANGEEGGKAGEGTVTREVRRLMGLNEEQEVPTDPRELASCVLHTCFMGTDNSSSATRGRAATLADQIGAYHNTITIDTAVSAVVGVFCALTGKTPQFLSRGGSSAEDLALQNIQARLRMVMAYLLAQLLPWVRGRQGFLLVLGSANVDEALRGYMTKYDCSSADLNPIGGISKVDLKRLLAWAARERDYPALAEIAAAPPTAELRPLAEGADEADEHSQVDEEDMGMTYEELSHFGRLRKVARCGPVSMFQNLLSAWRHLSPAEIAAKVKRFFFFYSVNRHKMTTLTPSYHAEEYSPDDNRFDLRPFLYPTRWPRQFAAIDSMLKGL 1941
            + D TDDILCDIGMPV H GVRYNCRVFCLNRKI+ IRPKL+LADDGNYRE RWFTTWK  ++    +  H L   + ++TGQ +VPFG  AI+A D++I+ ETCEE++TPDSPHI   L GVEIIGNGSGSHH+LRKL  R+  +  ATAK GGVY+Y+NQ+GCDGGRLYYDGCALIVVNG++VAQ +QFS+ DVEVITA V+L+DVRSYR + SSR EQAS  D R L  V+ P   C  T  + ++   PT    +R+H+PEEE ALGPACWLWDYLRRS + GFFLPLSGGADS+SVAAIVGVMC LA  AA                   AG+  V R++  L+        P +P  LA+ VLHT +MGT+NSS AT+ RA  LA++IG+YH  +TIDT VSAVV  F  L GKTPQF   GG+ +EDLALQNIQARLRMVMAYLLAQLLPWVR R GFLLVLGSANVDEALRGYMTKYDCSSADLNPIG +SK DLKR LA+AA++  YP LAEI AAPPTAELRP +       EH+QVDEEDMGMTYEELS FGRLRK+ R GP+ MF+ L + W HLS  EIAAKVKRFFF+Y VNRHKMTTLTPSYHAE YSPDDNRFDLRPFLY ++W RQFA+ID++   +
Sbjct:   76 ESDVTDDILCDIGMPVMHAGVRYNCRVFCLNRKIVFIRPKLYLADDGNYREKRWFTTWKIHHDHSLNLTTHMLPPLIQQLTGQVEVPFGIGAIAARDSVISTETCEELFTPDSPHISLALGGVEIIGNGSGSHHELRKLQHRIDLIRGATAKAGGVYLYANQQGCDGGRLYYDGCALIVVNGHIVAQGSQFSVADVEVITAVVDLDDVRSYRGAFSSRSEQASLPDQRSLSRVDLPFKLCQPTSGSKFLV--PTSRIDVRIHTPEEEIALGPACWLWDYLRRSKAKGFFLPLSGGADSASVAAIVGVMCHLATTAA------------------NAGDTQVQRDILYLLDREGSPYEPLEPATLANAVLHTTYMGTENSSIATKTRATRLAEEIGSYHLNMTIDTMVSAVVTTFSMLVGKTPQFGGHGGTPSEDLALQNIQARLRMVMAYLLAQLLPWVRSRSGFLLVLGSANVDEALRGYMTKYDCSSADLNPIGAVSKTDLKRFLAYAAQKYSYPTLAEIVAAPPTAELRPTSG----TGEHTQVDEEDMGMTYEELSWFGRLRKIDRSGPLWMFRKLGAIWTHLSSTEIAAKVKRFFFYYGVNRHKMTTLTPSYHAENYSPDDNRFDLRPFLYNSKWTRQFASIDALATDM 705          

BLAST of mRNA_M-pyrifera_M_contig1277.5578.1 vs. uniprot
Match: A0A5D6YPV6_9STRA (Glutamine-dependent NAD(+) synthetase n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6YPV6_9STRA)

HSP 1 Score: 816 bits (2108), Expect = 1.620e-289
Identity = 424/655 (64.73%), Postives = 488/655 (74.50%), Query Frame = 1

Query:    7 DATDDILCDIGMPVQHLGVRYNCRVFCLNRKILAIRPKLHLADDGNYRETRWFTTWK--RRNEMEDHTLCRGLAEVTGQDKVPFGQAAISALDALIAGETCEEIWTPDSPHIGQTLAGVEIIGNGSGSHHQLRKLDTRLSYMISATAKCGGVYVYSNQRGCDGGRLYYDGCALIVVNGNVVAQAAQFSLEDVEVITATVNLEDVRSYRASVSSRMEQASDARRL-PTVEAPL-FCLGTPDANYITHPPTQPQALRMHSPEEECALGPACWLWDYLRRSGSAGFFLPLSGGADSSSVAAIVGVMCGLAVDAAAAEKAELVAAAANGEEGGKAGEGTVTREVRRLMGLNEEQEVPTDPRELASCVLHTCFMGTDNSSSATRGRAATLADQIGAYHNTITIDTAVSAVVGVFCALTGKTPQFLSRGGSSAEDLALQNIQARLRMVMAYLLAQLLPWVRGRQGFLLVLGSANVDEALRGYMTKYDCSSADLNPIGGISKVDLKRLLAWAARERDYPALAEIAAAPPTAELRPLAEGADEADEHSQVDEEDMGMTYEELSHFGRLRKVARCGPVSMFQNLLSAWRHLSPAEIAAKVKRFFFFYSVNRHKMTTLTPSYHAEEYSPDDNRFDLRPFLYPTRWPRQFAAIDSMLKGLPPGRID 1959
            D T  ILCDIGMPV H GVRYNCRV+ L+++IL IRPKL LADDGNYRETRWFT WK   R  +EDH L   +   TGQ +VPFG  A++ LD   A ETCEE++TPDSPHI   LAGVEIIGNGSGSHHQLRKLD R+  +  AT K GGVY+Y+NQ+GCDGGRLYYDGCA+IVVNG VVAQ +QFS+ DVEV+TATV+L+DVRSYR SVSSR EQAS    + P +      C    DA   T PP    A+R H PEEE ALGPACW+WDYLRRSG +GFFLPLSGGADSSSVA IVGVMC L V+AA                   AG+  V ++V+R+ G   E   P   +ELA+ +LHT +MGT NSS+AT+ RAA+LA +IG YH ++ +D  V AVV  F  LTGK+P+FLS GGS  +DLALQNIQARLRMVMAYLLAQLLPWVR + GFLLVL SANVDEALRGYMTKYDCSS DLNPIG +SK DLK+LL W A   +YP L  +  APPTAELRP A+ ADEA +HSQ+DEEDMGM+YEEL  FGRLRK+ RCGPV MF+ L   W HL+P  +A KVKRFFF+YSVNRHKMTTLTPSYHAE YSPDDNRFDLRPFLY  RWPRQF AID++   L   + D
Sbjct:   77 DVTAGILCDIGMPVMHNGVRYNCRVYVLDQQILFIRPKLFLADDGNYRETRWFTAWKPGTRRGLEDHVLPPRIQRATGQVRVPFGAGALATLDTCCASETCEELFTPDSPHINLGLAGVEIIGNGSGSHHQLRKLDQRMDLIRGATTKSGGVYLYANQQGCDGGRLYYDGCAVIVVNGQVVAQGSQFSVADVEVVTATVDLDDVRSYRGSVSSRSEQASALDTVVPKINVAFSLCHSDDDAGRATSPPI---AVRYHEPEEEIALGPACWMWDYLRRSGGSGFFLPLSGGADSSSVACIVGVMCHLVVEAA------------------NAGDARVIQDVQRVTGTTNEVYAPLTAQELAAHLLHTTYMGTKNSSAATKHRAASLAKEIGCYHLSMGMDMMVDAVVKTFEVLTGKSPRFLSHGGSIQQDLALQNIQARLRMVMAYLLAQLLPWVRAKTGFLLVLSSANVDEALRGYMTKYDCSSGDLNPIGAVSKGDLKKLLRWGAVRYNYPTLQTVVEAPPTAELRPTADSADEAADHSQLDEEDMGMSYEELGWFGRLRKIDRCGPVGMFRKLTHLWGHLAPTVVAEKVKRFFFYYSVNRHKMTTLTPSYHAENYSPDDNRFDLRPFLYNVRWPRQFRAIDTLAAQLEETKKD 710          

BLAST of mRNA_M-pyrifera_M_contig1277.5578.1 vs. uniprot
Match: G4YVY0_PHYSP (Glutamine-dependent NAD(+) synthetase n=2 Tax=Peronosporaceae TaxID=4777 RepID=G4YVY0_PHYSP)

HSP 1 Score: 813 bits (2100), Expect = 2.840e-288
Identity = 422/652 (64.72%), Postives = 493/652 (75.61%), Query Frame = 1

Query:    7 DATDDILCDIGMPVQHLGVRYNCRVFCLNRKILAIRPKLHLADDGNYRETRWFTTWKRRNE------MEDHTLCRGLAEVTGQDKVPFGQAAISALDALIAGETCEEIWTPDSPHIGQTLAGVEIIGNGSGSHHQLRKLDTRLSYMISATAKCGGVYVYSNQRGCDGGRLYYDGCALIVVNGNVVAQAAQFSLEDVEVITATVNLEDVRSYRASVSSRMEQASDARR-LPTVEAPLFCLGTPDANYITHPPTQPQALRMHSPEEECALGPACWLWDYLRRSGSAGFFLPLSGGADSSSVAAIVGVMCGLAVDAAAAEKAELVAAAANGEEGGKAGEGTVTREVRRLMGLNEEQEVPTDPRELASCVLHTCFMGTDNSSSATRGRAATLADQIGAYHNTITIDTAVSAVVGVFCALTGKTPQFLSRGGSSAEDLALQNIQARLRMVMAYLLAQLLPWVRGRQGFLLVLGSANVDEALRGYMTKYDCSSADLNPIGGISKVDLKRLLAWAARERDYPALAEIAAAPPTAELRPLAEGADEADEHSQVDEEDMGMTYEELSHFGRLRKVARCGPVSMFQNLLSAWRHLSPAEIAAKVKRFFFFYSVNRHKMTTLTPSYHAEEYSPDDNRFDLRPFLYPTRWPRQFAAIDSMLKGL 1941
            D T+DILCDIGMPV H GVRYNCRVFCLN++IL IRPKL LADDGNYRE RWFTTWK   E      ++   L   L ++TG   VPFG  A+S LD   A ETCEE++TPDSPHI  +LAGVEIIGNGSGSHHQLRKLD R+  +  AT K GGVY+Y+NQ+GCDGGRLY+DGCA+IVVNG VVAQ +QFS++DVEV+TATV+L+DVRSYR SVSSR EQAS     +P ++   F L   +A+++   PT    +R H PEEE ALGPACW+WDYLRRSG +GFFLPLSGGADSSSVA IVGVMC L V+AA                    G+  V ++V+R+MG + ++  P  P +LAS VLHT +MGT NSS+AT+ RAATLA +IG YH  + +D  V AVV  F  LTGKTPQ+LSRGG+  EDLALQNIQARLRMVMAYLLAQLLPWVR + GFLLVL S NVDEALRGYMTKYDCSS DLNPIG +SK DLK+LL WAA + +YPAL  +  APPTAELRP  E A E  +HSQ+DEEDMGMTY+EL  FGRLRK+ RCGP  MF+ L + W HL+P  +A KVKRFFF+YSVNRHKMTTLTPSYHAE YSPDDNRFDLRPFLY +RW RQF +ID++   L
Sbjct:   77 DVTNDILCDIGMPVMHNGVRYNCRVFCLNQRILFIRPKLFLADDGNYREKRWFTTWKPSLETGMSRGLDKFVLPASLQKLTGAVHVPFGSGAVSTLDTSCASETCEELFTPDSPHINLSLAGVEIIGNGSGSHHQLRKLDQRMDLIRGATTKSGGVYLYANQQGCDGGRLYFDGCAVIVVNGQVVAQGSQFSVKDVEVVTATVDLDDVRSYRGSVSSRSEQASSLDTVIPKIDVD-FSLCHDEASFVH--PTPAIEVRYHVPEEEIALGPACWMWDYLRRSGGSGFFLPLSGGADSSSVACIVGVMCHLVVEAA------------------NKGDEQVIKDVQRIMGTSGQEYKPLKPADLASHVLHTTYMGTKNSSAATKKRAATLASEIGCYHLNMGMDMMVDAVVKTFELLTGKTPQYLSRGGTLQEDLALQNIQARLRMVMAYLLAQLLPWVRSKTGFLLVLSSGNVDEALRGYMTKYDCSSGDLNPIGAVSKGDLKKLLRWAAVKYNYPALQTVEEAPPTAELRPTDESAGEDADHSQLDEEDMGMTYDELGFFGRLRKIDRCGPYWMFRKLTNLWSHLAPTVVATKVKRFFFYYSVNRHKMTTLTPSYHAENYSPDDNRFDLRPFLYNSRWTRQFNSIDTLATKL 707          

BLAST of mRNA_M-pyrifera_M_contig1277.5578.1 vs. uniprot
Match: A0A8K1CI43_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1CI43_PYTOL)

HSP 1 Score: 810 bits (2091), Expect = 6.840e-287
Identity = 418/652 (64.11%), Postives = 486/652 (74.54%), Query Frame = 1

Query:    7 DATDDILCDIGMPVQHLGVRYNCRVFCLNRKILAIRPKLHLADDGNYRETRWFTTWKRRNEMED-----HTLCRGLAEVTGQDKVPFGQAAISALDALIAGETCEEIWTPDSPHIGQTLAGVEIIGNGSGSHHQLRKLDTRLSYMISATAKCGGVYVYSNQRGCDGGRLYYDGCALIVVNGNVVAQAAQFSLEDVEVITATVNLEDVRSYRASVSSRMEQASDARR-LPTVEAPLFCLGTPDANYITHPPTQPQALRMHSPEEECALGPACWLWDYLRRSGSAGFFLPLSGGADSSSVAAIVGVMCGLAVDAAAAEKAELVAAAANGEEGGKAGEGTVTREVRRLMGLNEEQEVPTDPRELASCVLHTCFMGTDNSSSATRGRAATLADQIGAYHNTITIDTAVSAVVGVFCALTGKTPQFLSRGGSSAEDLALQNIQARLRMVMAYLLAQLLPWVRGRQGFLLVLGSANVDEALRGYMTKYDCSSADLNPIGGISKVDLKRLLAWAARERDYPALAEIAAAPPTAELRPLAEGADE-ADEHSQVDEEDMGMTYEELSHFGRLRKVARCGPVSMFQNLLSAWRHLSPAEIAAKVKRFFFFYSVNRHKMTTLTPSYHAEEYSPDDNRFDLRPFLYPTRWPRQFAAIDSMLKGL 1941
            D TD ILCDIGMPV H GVRYNCR++CLN+KIL IRPKL LADDGNYRETRWFT WK   E +D     H L R + ++TGQ  VPFG  A+S LD   + ETCEE++TPDSPHI  +LAGVEII NGSGSHHQLRKL  RL  +  AT K GG+Y+Y+NQ+GCDGGRLYYDGCALI VNG VVAQ  QFS++DVEV+TATV+L+DVRSYR S+SSR EQAS  +  LPT+          D   ++  PT+P  L  H PEEE ALGPACWLWDYLRRSG  G+FLPLSGGADSSSVA IVGVMC             LV  AANG      G+  V R+V+R+MG   E+  P  P++LAS VLHT +MGT NSS+AT+ RA+TLA ++G YH  + +DT V AV+  F  LTGKTP+F+S GG+  EDLALQNIQARLRMVMAYLLAQLLPWVR + GFLLVL SANVDEALRGYMTKYDCSS DLNPIG +SK DLK+LL WAA + +YP L  +  APPTAELRP    +D+   +HSQ+DEEDMGM+YEEL +FGRLRK+ R GP  M++ L   W HL+P  +A KVKRFFF+YS+NRHKMTTLTPSYHAE YSPDDNRFD RPFLY  RW RQF AIDS+   L
Sbjct:   77 DETDGILCDIGMPVMHNGVRYNCRLYCLNKKILFIRPKLFLADDGNYRETRWFTAWKVNKENKDQMLQQHVLPRNIQKITGQVHVPFGYGALSTLDTTCSSETCEELFTPDSPHINLSLAGVEIISNGSGSHHQLRKLHQRLELIRGATTKGGGIYLYANQQGCDGGRLYYDGCALIAVNGEVVAQGTQFSVKDVEVVTATVDLDDVRSYRGSISSRSEQASALKTVLPTIHVDFSLCH--DEEDLSAAPTRPIDLHYHVPEEEIALGPACWLWDYLRRSGGGGYFLPLSGGADSSSVACIVGVMC------------HLVTEAANG------GDPQVIRDVQRIMGTTGEEYKPLTPQDLASHVLHTTYMGTKNSSAATKKRASTLAQELGCYHLNMGMDTMVDAVINTFALLTGKTPRFMSLGGTIQEDLALQNIQARLRMVMAYLLAQLLPWVRSKNGFLLVLSSANVDEALRGYMTKYDCSSGDLNPIGAVSKGDLKKLLRWAAVKYNYPTLQTVVDAPPTAELRPTGNASDDDTTDHSQLDEEDMGMSYEELGYFGRLRKIDRSGPFWMYRKLTHIWSHLTPTVVAEKVKRFFFYYSINRHKMTTLTPSYHAENYSPDDNRFDHRPFLYNARWTRQFRAIDSLATKL 708          

BLAST of mRNA_M-pyrifera_M_contig1277.5578.1 vs. uniprot
Match: A0A0P1ABT4_PLAHL (Glutamine-dependent NAD(+) synthetase n=1 Tax=Plasmopara halstedii TaxID=4781 RepID=A0A0P1ABT4_PLAHL)

HSP 1 Score: 804 bits (2077), Expect = 8.790e-285
Identity = 418/652 (64.11%), Postives = 486/652 (74.54%), Query Frame = 1

Query:    7 DATDDILCDIGMPVQHLGVRYNCRVFCLNRKILAIRPKLHLADDGNYRETRWFTTWK------RRNEMEDHTLCRGLAEVTGQDKVPFGQAAISALDALIAGETCEEIWTPDSPHIGQTLAGVEIIGNGSGSHHQLRKLDTRLSYMISATAKCGGVYVYSNQRGCDGGRLYYDGCALIVVNGNVVAQAAQFSLEDVEVITATVNLEDVRSYRASVSSRMEQASDARR-LPTVEAPLFCLGTPDANYITHPPTQPQALRMHSPEEECALGPACWLWDYLRRSGSAGFFLPLSGGADSSSVAAIVGVMCGLAVDAAAAEKAELVAAAANGEEGGKAGEGTVTREVRRLMGLNEEQEVPTDPRELASCVLHTCFMGTDNSSSATRGRAATLADQIGAYHNTITIDTAVSAVVGVFCALTGKTPQFLSRGGSSAEDLALQNIQARLRMVMAYLLAQLLPWVRGRQGFLLVLGSANVDEALRGYMTKYDCSSADLNPIGGISKVDLKRLLAWAARERDYPALAEIAAAPPTAELRPLAEGADEADEHSQVDEEDMGMTYEELSHFGRLRKVARCGPVSMFQNLLSAWRHLSPAEIAAKVKRFFFFYSVNRHKMTTLTPSYHAEEYSPDDNRFDLRPFLYPTRWPRQFAAIDSMLKGL 1941
            D T+DILCDIGMPV H GVRYNCRVFCLN++I+ IRPKL LADDGNYRETRWFTTWK          ++   L   L ++TG   VPFG  A+S LD   A ETCEE++TPDSPHI  +LAGVEI GNGSGSHHQLRKL  R+  +  AT K GGVY+Y+NQ+GCDGGRLYYDGCA+IVVNG VVAQ +QFS+ DVEVITATV+L+DVRSYR SVSSR EQAS     +P ++   F L   D   +   PT P  ++ H PEEE ALGPACW+WDYLRRSG +GFFLPLSGGADSSSVA IVGVMC L VDAA                    G   V R+V+R++G ++       P +LAS +L+T +MGT NSS+AT+ RAATLA +IG YH ++ +D  V AVV  F  LTGKTPQFLS+GG+  EDLALQNIQARLRMVMAYLLAQLLPWVR + G+LLVL S NVDEALRGYMTKYDCSS DLNPIG  SK DLK+LL WAA +  YP+L  I  APPTAELRP  E A+E   HSQ+DE+DMGMTY+EL  FGRLRK+ RCGP  MFQ L++ W H++P E+A KVKRFF++YSVNRHKMTTLTPSYHAE YSPDDNRFDLRPFLY TRW RQF +ID ++  L
Sbjct:   77 DVTNDILCDIGMPVMHNGVRYNCRVFCLNQRIVFIRPKLFLADDGNYRETRWFTTWKPNLDKSNARSLDRFVLPASLQKLTGVVHVPFGSGAVSTLDTSCASETCEELFTPDSPHINLSLAGVEIFGNGSGSHHQLRKLHQRMDLIRGATTKSGGVYLYANQQGCDGGRLYYDGCAVIVVNGQVVAQGSQFSVNDVEVITATVDLDDVRSYRGSVSSRSEQASSLDTVIPKIDVN-FSLCHDDM--VIAQPTAPIEVQYHIPEEEIALGPACWMWDYLRRSGGSGFFLPLSGGADSSSVACIVGVMCHLVVDAA------------------NDGNEQVIRDVQRIIGTSDRMYQRLTPEDLASQILYTTYMGTKNSSAATKKRAATLASEIGCYHLSMGMDIVVDAVVKTFSLLTGKTPQFLSKGGTMQEDLALQNIQARLRMVMAYLLAQLLPWVRSKSGYLLVLSSGNVDEALRGYMTKYDCSSGDLNPIGATSKGDLKKLLRWAAVKYHYPSLQIIEEAPPTAELRPTDERAEEYANHSQLDEDDMGMTYDELGFFGRLRKIDRCGPYWMFQKLINLWSHVTPTEVAVKVKRFFYYYSVNRHKMTTLTPSYHAENYSPDDNRFDLRPFLYNTRWTRQFDSIDKLVAKL 707