mRNA_M-pyrifera_M_contig127118.5468.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig127118.5468.1
Unique NamemRNA_M-pyrifera_M_contig127118.5468.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig127118.5468.1 vs. uniprot
Match: A0A6L8EIE0_9GAMM (Cytochrome b5 heme-binding domain-containing protein n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A6L8EIE0_9GAMM)

HSP 1 Score: 253 bits (646), Expect = 1.530e-81
Identity = 119/140 (85.00%), Postives = 127/140 (90.71%), Query Frame = 1 
Query:   85 MDFNPINSSSMDARKSEFGYVVTYWVRDSELLNVWFGYYEPNADEFERGEGDWFPADRTEMIANVKKAFANDVRMDDAVALVGGVENPTKWGLYDLDAFPSWHNGRIAVLGDAAHAVLPTFGQGAALAIEDGAALGKCFE 504
            MD+NPI+S+SMDARK+EFGYVVTYWVR  ELLNVW GYYEP+ADEFE  EGDWFPADRTEMI N+K AF +DVRMDD VAL G V  PTKWGLYDLDAFPSWHNGR+AVLGDAAHAVLPTFGQGAALAIEDGAALGKCFE
Sbjct:    1 MDYNPIDSASMDARKTEFGYVVTYWVRGGELLNVWMGYYEPDADEFEGDEGDWFPADRTEMIRNMKIAFKDDVRMDDVVALAGSVVKPTKWGLYDLDAFPSWHNGRVAVLGDAAHAVLPTFGQGAALAIEDGAALGKCFE 140
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BLAST of mRNA_M-pyrifera_M_contig127118.5468.1 vs. uniprot
Match: A7KS54_9BURK (Ortho-nitrophenol 2-monooxygenase n=1 Tax=Alcaligenes sp. NyZ215 TaxID=441452 RepID=A7KS54_9BURK)

HSP 1 Score: 242 bits (617), Expect = 1.370e-74
Identity = 112/168 (66.67%), Postives = 127/168 (75.60%), Query Frame = 1 
Query:    1 RRWTFVVCYRGLIPRDKVAELTKEDGSPMDFNPINSSSMDARKSEFGYVVTYWVRDSELLNVWFGYYEPNADEFERGEGDWFPADRTEMIANVKKAFANDVRMDDAVALVGGVENPTKWGLYDLDAFPSWHNGRIAVLGDAAHAVLPTFGQGAALAIEDGAALGKCFE 504
            RRWT V CYRGLIPRDKVA L K DG P+DFNPI+S SMD+RK    + +TYWVR  ELLNVW  YYEPNA EFE  EGDWFPAD  E    + KAF++D R DD VAL G +ENPTKWGLYD DA   WH+GR+ +LGDAAH++LPTFGQGA  A EDGAAL  CF+
Sbjct:  193 RRWTEVTCYRGLIPRDKVAALKKADGGPLDFNPIDSFSMDSRKHPRAWAMTYWVRGGELLNVWLAYYEPNAKEFEGDEGDWFPADHAEFQEKMSKAFSDDPRKDDVVALAGAMENPTKWGLYDRDALEHWHDGRVCLLGDAAHSMLPTFGQGANQAFEDGAALSSCFK 360
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BLAST of mRNA_M-pyrifera_M_contig127118.5468.1 vs. uniprot
Match: UPI001EE3702A (FAD-dependent monooxygenase n=1 Tax=Nocardioides cynanchi TaxID=2558918 RepID=UPI001EE3702A)

HSP 1 Score: 236 bits (603), Expect = 3.650e-72
Identity = 110/168 (65.48%), Postives = 127/168 (75.60%), Query Frame = 1 
Query:    1 RRWTFVVCYRGLIPRDKVAELTKEDGSPMDFNPINSSSMDARKSEFGYVVTYWVRDSELLNVWFGYYEPNADEFERGEGDWFPADRTEMIANVKKAFANDVRMDDAVALVGGVENPTKWGLYDLDAFPSWHNGRIAVLGDAAHAVLPTFGQGAALAIEDGAALGKCFE 504
            +RWT VV YRGL+PRDKVA   K DGSP+DFNPI + SMD+R++     +TYWVR  ELLNVW  YYEPNA+EFER EGDWFPADR EM+  + KAF  D R DD V L G +ENPTKWGLYD DA  SW  GR+ ++GDAAH +LPT+GQGAA A ED AALGK FE
Sbjct:  214 KRWTEVVVYRGLVPRDKVAAARKPDGSPLDFNPIETMSMDSRETPVAQHLTYWVRGGELLNVWLAYYEPNAEEFEREEGDWFPADRDEMVGQMSKAFEGDPRHDDVVTLTGLIENPTKWGLYDRDALESWVEGRVGLVGDAAHPMLPTYGQGAAQAFEDAAALGKAFE 381
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BLAST of mRNA_M-pyrifera_M_contig127118.5468.1 vs. uniprot
Match: UPI00054B9404 (FAD-dependent monooxygenase n=2 Tax=Pseudomonas fluorescens TaxID=294 RepID=UPI00054B9404)

HSP 1 Score: 230 bits (586), Expect = 5.570e-70
Identity = 109/168 (64.88%), Postives = 120/168 (71.43%), Query Frame = 1 
Query:    1 RRWTFVVCYRGLIPRDKVAELTKEDGSPMDFNPINSSSMDARKSEFGYVVTYWVRDSELLNVWFGYYEPNADEFERGEGDWFPADRTEMIANVKKAFANDVRMDDAVALVGGVENPTKWGLYDLDAFPSWHNGRIAVLGDAAHAVLPTFGQGAALAIEDGAALGKCFE 504
            RRWT V CYRGLIPRDKVA L K DGSP+D NPI+S SMD+RK    + + YWVR  ELLNVW  YYEPNA EFE  EGDWF AD  E    + KAF  D R DD  AL G +ENP+KWGLYD DAF  WH GRI +LGDAAH +LPTFGQG   A EDGAAL  CF+
Sbjct:  193 RRWTEVTCYRGLIPRDKVAALRKADGSPLDHNPIDSFSMDSRKHLTAWAMNYWVRGGELLNVWLAYYEPNAAEFEGDEGDWFSADPAEFQEKMNKAFIGDPRRDDVTALAGAMENPSKWGLYDRDAFEHWHEGRIGLLGDAAHPMLPTFGQGVNQAFEDGAALSSCFK 360
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BLAST of mRNA_M-pyrifera_M_contig127118.5468.1 vs. uniprot
Match: A0A6L8EIF9_9GAMM (Monooxygenase n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A6L8EIF9_9GAMM)

HSP 1 Score: 225 bits (573), Expect = 4.730e-68
Identity = 107/168 (63.69%), Postives = 126/168 (75.00%), Query Frame = 1 
Query:    1 RRWTFVVCYRGLIPRDKVAELTKEDGSPMDFNPINSSSMDARKSEFGYVVTYWVRDSELLNVWFGYYEPNADEFERGEGDWFPADRTEMIANVKKAFANDVRMDDAVALVGGVENPTKWGLYDLDAFPSWHNGRIAVLGDAAHAVLPTFGQGAALAIEDGAALGKCFE 504
            RRWT V  YRGLI R+ VA L K DGSP+D NPI+S SMD RK+ + + +TYWVR  ELLNVW  YYEP++ EFE  EGDWF  D +E+ AN+ +AFA+DVR DD VAL G VENPTKWGLYD DAF  W +GRI +LGDAAH +LPT GQGA+ A ED AAL  CF+
Sbjct:  193 RRWTEVTVYRGLIKREDVAALRKADGSPLDNNPIDSFSMDVRKNPYAWALTYWVRGGELLNVWQAYYEPDSAEFEADEGDWFHVDNSELQANMGRAFADDVRKDDIVALSGAVENPTKWGLYDRDAFEHWQDGRICLLGDAAHPMLPTMGQGASQAFEDAAALSSCFK 360
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BLAST of mRNA_M-pyrifera_M_contig127118.5468.1 vs. uniprot
Match: UPI001E2E7EE2 (FAD-dependent monooxygenase n=1 Tax=Bradyrhizobium altum TaxID=1571202 RepID=UPI001E2E7EE2)

HSP 1 Score: 221 bits (563), Expect = 1.350e-66
Identity = 100/168 (59.52%), Postives = 127/168 (75.60%), Query Frame = 1 
Query:    1 RRWTFVVCYRGLIPRDKVAELTKEDGSPMDFNPINSSSMDARKSEFGYVVTYWVRDSELLNVWFGYYEPNADEFERGEGDWFPADRTEMIANVKKAFANDVRMDDAVALVGGVENPTKWGLYDLDAFPSWHNGRIAVLGDAAHAVLPTFGQGAALAIEDGAALGKCFE 504
            RRWT V+CYRGLI R+ VA+LTK+DG PMD NPI+S SMD  K++  + +TYWVR  ELLNVW  YYEPNA++F+    DW+P D  +MI ++ K F +D R +D +A+   +E+PTKWGLYD DAF SWH GR+ +LGDAAH +LPTFGQGA+ + ED AALGK FE
Sbjct:  193 RRWTEVICYRGLIAREDVAKLTKQDGRPMDNNPIDSFSMDVIKNDVAFCMTYWVRGGELLNVWLAYYEPNAEDFQAD--DWYPIDHADMIKHMNKGFGDDPRKEDVLAMAAAMESPTKWGLYDRDAFESWHQGRVCLLGDAAHPMLPTFGQGASQSFEDAAALGKAFE 358
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BLAST of mRNA_M-pyrifera_M_contig127118.5468.1 vs. uniprot
Match: UPI0014901B68 (FAD-dependent monooxygenase n=1 Tax=Ensifer adhaerens TaxID=106592 RepID=UPI0014901B68)

HSP 1 Score: 214 bits (546), Expect = 4.790e-64
Identity = 101/168 (60.12%), Postives = 123/168 (73.21%), Query Frame = 1 
Query:    1 RRWTFVVCYRGLIPRDKVAELTKEDGSPMDFNPINSSSMDARKSEFGYVVTYWVRDSELLNVWFGYYEPNADEFERGEGDWFPADRTEMIANVKKAFANDVRMDDAVALVGGVENPTKWGLYDLDAFPSWHNGRIAVLGDAAHAVLPTFGQGAALAIEDGAALGKCFE 504
            RRWT VVCYRGLIPR +VA L K DGSP+D NPI+S SMD+RK    + +TYWVR  ELLNVW  YYEP++ EF    GDW P + + ++ ++++AF  D R DD VAL   +ENPTKWGLYD DA   W +GRI +LGDAAH +LPTFGQGAA + EDGAAL  CF+
Sbjct:  196 RRWTEVVCYRGLIPRAQVAALRKLDGSPLDHNPIDSFSMDSRKDNRAWGMTYWVRGGELLNVWLAYYEPDSREFTEDWGDWRPIENSVVMESMQRAFDGDPRKDDMVALASAMENPTKWGLYDRDALEQWQDGRICLLGDAAHPMLPTFGQGAAQSFEDGAALSSCFK 363
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BLAST of mRNA_M-pyrifera_M_contig127118.5468.1 vs. uniprot
Match: A0A1Z9J9U7_9PROT (Monooxygenase n=1 Tax=Rhodospirillaceae bacterium TMED167 TaxID=1986863 RepID=A0A1Z9J9U7_9PROT)

HSP 1 Score: 210 bits (534), Expect = 2.950e-62
Identity = 97/168 (57.74%), Postives = 120/168 (71.43%), Query Frame = 1 
Query:    1 RRWTFVVCYRGLIPRDKVAELTKEDGSPMDFNPINSSSMDARKSEFGYVVTYWVRDSELLNVWFGYYEPNADEFERGEGDWFPADRTEMIANVKKAFANDVRMDDAVALVGGVENPTKWGLYDLDAFPSWHNGRIAVLGDAAHAVLPTFGQGAALAIEDGAALGKCFE 504
            RRWT V+C+RGLI RD VA L K DG P+D NPI+S SMD  K+E   V +YWVR  ELLNVW  +YEP++ EF++ EGDWFP  + E+   V++AFA     DD +AL G ++ PTKWGLYD DA  +W  GR+ +LGDAAH +LPTFGQGAA + EDGAAL   FE
Sbjct:  193 RRWTGVICFRGLIARDDVAVLRKADGRPLDNNPIDSFSMDRHKTERSAVTSYWVRSGELLNVWIAHYEPDSKEFKQEEGDWFPVSQQEVADQVREAFAESPNRDDLIALAGAIDRPTKWGLYDRDALDTWVQGRVCLLGDAAHPMLPTFGQGAAQSFEDGAALSSAFE 360
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BLAST of mRNA_M-pyrifera_M_contig127118.5468.1 vs. uniprot
Match: Q5LNQ0_RUEPO (Monooxygenase, putative n=4 Tax=Ruegeria TaxID=97050 RepID=Q5LNQ0_RUEPO)

HSP 1 Score: 206 bits (525), Expect = 6.510e-61
Identity = 101/167 (60.48%), Postives = 116/167 (69.46%), Query Frame = 1 
Query:    1 RRWTFVVCYRGLIPRDKVAELTKEDGSPMDFNPINSSSMDARKSEFGYVVTYWVRDSELLNVWFGYYEPNADEFERGEGDWFPADRTEMIANVKKAFANDVRMDDAVALVGGVENPTKWGLYDLDAFPSWHNGRIAVLGDAAHAVLPTFGQGAALAIEDGAALGKCF 501
            RRWT V C+RGLIPR+ VA L K DGSP+D+NPI+S SMD  K++     TYWVR  ELLNVW   YEP++  FE+ EGDWFP  R E+  +V  AFA     DD VAL G +  PTKWGLYD DA  SW  GRI +LGDAAH +LPTFGQGAA A ED AALG  F
Sbjct:  193 RRWTEVTCFRGLIPRETVAALRKPDGSPLDYNPIHSFSMDRHKTDRSGATTYWVRGGELLNVWIARYEPDSTAFEQEEGDWFPISREEITRDVGAAFAELPNRDDLVALAGAMVRPTKWGLYDRDALDSWVQGRICLLGDAAHPMLPTFGQGAAQAFEDAAALGCAF 359
[Close]
BLAST of mRNA_M-pyrifera_M_contig127118.5468.1 vs. uniprot
Match: A0A3D5DD50_9GAMM (Monooxygenase n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A3D5DD50_9GAMM)

HSP 1 Score: 206 bits (524), Expect = 7.980e-61
Identity = 99/168 (58.93%), Postives = 113/168 (67.26%), Query Frame = 1 
Query:    1 RRWTFVVCYRGLIPRDKVAELTKEDGSPMDFNPINSSSMDARKSEFGYVVTYWVRDSELLNVWFGYYEPNADEFERGEGDWFPADRTEMIANVKKAFANDVRMDDAVALVGGVENPTKWGLYDLDAFPSWHNGRIAVLGDAAHAVLPTFGQGAALAIEDGAALGKCFE 504
            RRWT V  YRGL+PR     L   DGSP+D NPI S SMD RK    + +TYWVR  ELLNVW  Y+EP++ EF    GDWFP D  EM   + KAFA D R DD +AL  G+  PTKWGLYD DAF  W  GR+ +LGDAAH +LPTFGQGAA A EDGAAL  CF+
Sbjct:  193 RRWTEVTVYRGLVPRATFDTLRHADGSPLDNNPIESYSMDVRKHPHAWCMTYWVRSGELLNVWLAYHEPDSAEFPEDWGDWFPIDHGEMGEKMGKAFAGDPRRDDILALASGMVKPTKWGLYDRDAFEYWQEGRVCLLGDAAHPMLPTFGQGAAQAFEDGAALSSCFK 360
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig127118.5468.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6L8EIE0_9GAMM1.530e-8185.00Cytochrome b5 heme-binding domain-containing prote... [more]
A7KS54_9BURK1.370e-7466.67Ortho-nitrophenol 2-monooxygenase n=1 Tax=Alcalige... [more]
UPI001EE3702A3.650e-7265.48FAD-dependent monooxygenase n=1 Tax=Nocardioides c... [more]
UPI00054B94045.570e-7064.88FAD-dependent monooxygenase n=2 Tax=Pseudomonas fl... [more]
A0A6L8EIF9_9GAMM4.730e-6863.69Monooxygenase n=1 Tax=Gammaproteobacteria bacteriu... [more]
UPI001E2E7EE21.350e-6659.52FAD-dependent monooxygenase n=1 Tax=Bradyrhizobium... [more]
UPI0014901B684.790e-6460.12FAD-dependent monooxygenase n=1 Tax=Ensifer adhaer... [more]
A0A1Z9J9U7_9PROT2.950e-6257.74Monooxygenase n=1 Tax=Rhodospirillaceae bacterium ... [more]
Q5LNQ0_RUEPO6.510e-6160.48Monooxygenase, putative n=4 Tax=Ruegeria TaxID=970... [more]
A0A3D5DD50_9GAMM7.980e-6158.93Monooxygenase n=1 Tax=Gammaproteobacteria bacteriu... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig127118contigM-pyrifera_M_contig127118:3..506 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score205.3
Seed ortholog evalue2.2e-50
Seed eggNOG ortholog246200.SPO3153
Hectar predicted targeting categoryother localisation
EggNOG free text desc.heme binding
EggNOG OGs1MWWT@1224,2TRZP@28211,COG0654@1,COG0654@2,COG5274@1,COG5274@2
COG Functional cat.C
Best tax levelAlphaproteobacteria
Best eggNOG OGNA|NA|NA
Exons1
Model size504
Cds size504
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815785.4453793-CDS-M-pyrifera_M_contig127118:2..5061622815785.4453793-CDS-M-pyrifera_M_contig127118:2..506Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig127118 3..506 -
1692277568.4236505-CDS-M-pyrifera_M_contig127118:2..5061692277568.4236505-CDS-M-pyrifera_M_contig127118:2..506Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig127118 3..506 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig127118.5468.1prot_M-pyrifera_M_contig127118.5468.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig127118 3..506 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig127118.5468.1

>prot_M-pyrifera_M_contig127118.5468.1 ID=prot_M-pyrifera_M_contig127118.5468.1|Name=mRNA_M-pyrifera_M_contig127118.5468.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=168bp
RRWTFVVCYRGLIPRDKVAELTKEDGSPMDFNPINSSSMDARKSEFGYVV
TYWVRDSELLNVWFGYYEPNADEFERGEGDWFPADRTEMIANVKKAFAND
VRMDDAVALVGGVENPTKWGLYDLDAFPSWHNGRIAVLGDAAHAVLPTFG
QGAALAIEDGAALGKCFE
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mRNA from alignment at M-pyrifera_M_contig127118:3..506-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig127118.5468.1 ID=mRNA_M-pyrifera_M_contig127118.5468.1|Name=mRNA_M-pyrifera_M_contig127118.5468.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=504bp|location=Sequence derived from alignment at M-pyrifera_M_contig127118:3..506- (Macrocystis pyrifera P11B4 male)
CGGCGCTGGACATTTGTTGTGTGCTATCGGGGTCTGATTCCGCGCGACAA
GGTTGCCGAACTCACGAAGGAAGACGGTAGCCCGATGGACTTCAACCCGA
TTAATTCCTCGAGCATGGATGCCCGCAAATCCGAATTCGGTTACGTCGTC
ACCTATTGGGTGCGCGACAGCGAACTGCTCAACGTCTGGTTTGGTTACTA
CGAACCCAACGCTGACGAATTCGAACGCGGGGAGGGCGACTGGTTTCCCG
CTGACCGCACGGAAATGATAGCGAATGTGAAAAAGGCGTTCGCCAATGAT
GTGCGCATGGATGATGCCGTCGCCCTTGTGGGTGGCGTTGAGAATCCGAC
GAAGTGGGGCCTTTATGATCTCGATGCATTTCCGAGTTGGCACAATGGCC
GGATCGCGGTGTTAGGTGACGCGGCGCATGCCGTACTGCCGACTTTCGGC
CAGGGCGCCGCGCTCGCGATCGAAGACGGCGCCGCGCTCGGCAAATGTTT
CGAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig127118:3..506-

>mRNA_M-pyrifera_M_contig127118.5468.1 ID=mRNA_M-pyrifera_M_contig127118.5468.1|Name=mRNA_M-pyrifera_M_contig127118.5468.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1008bp|location=Sequence derived from alignment at M-pyrifera_M_contig127118:3..506- (Macrocystis pyrifera P11B4 male)
CGGCGCTGGACATTTGTTGTGTGCTATCGGGGTCTGATTCCGCGCGACAA
GGTTGCCGAACTCACGAAGGAAGACGGTAGCCCGATGGACTTCAACCCGA
TTAATTCCTCGAGCATGGATGCCCGCAAATCCGAATTCGGTTACGTCGTC
ACCTATTGGGTGCGCGACAGCGAACTGCTCAACGTCTGGTTTGGTTACTA
CGAACCCAACGCTGACGAATTCGAACGCGGGGAGGGCGACTGGTTTCCCG
CTGACCGCACGGAAATGATAGCGAATGTGAAAAAGGCGTTCGCCAATGAT
GTGCGCATGGATGATGCCGTCGCCCTTGTGGGTGGCGTTGAGAATCCGAC
GAAGTGGGGCCTTTATGATCTCGATGCATTTCCGAGTTGGCACAATGGCC
GGATCGCGGTGTTAGGTGACGCGGCGCATGCCGTACTGCCGACTTTCGGC
CAGGGCGCCGCGCTCGCGATCGAAGACGGCGCCGCGCTCGGCAAATGTTT
CGAACGGCGCTGGACATTTGTTGTGTGCTATCGGGGTCTGATTCCGCGCG
ACAAGGTTGCCGAACTCACGAAGGAAGACGGTAGCCCGATGGACTTCAAC
CCGATTAATTCCTCGAGCATGGATGCCCGCAAATCCGAATTCGGTTACGT
CGTCACCTATTGGGTGCGCGACAGCGAACTGCTCAACGTCTGGTTTGGTT
ACTACGAACCCAACGCTGACGAATTCGAACGCGGGGAGGGCGACTGGTTT
CCCGCTGACCGCACGGAAATGATAGCGAATGTGAAAAAGGCGTTCGCCAA
TGATGTGCGCATGGATGATGCCGTCGCCCTTGTGGGTGGCGTTGAGAATC
CGACGAAGTGGGGCCTTTATGATCTCGATGCATTTCCGAGTTGGCACAAT
GGCCGGATCGCGGTGTTAGGTGACGCGGCGCATGCCGTACTGCCGACTTT
CGGCCAGGGCGCCGCGCTCGCGATCGAAGACGGCGCCGCGCTCGGCAAAT
GTTTCGAA
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