mRNA_M-pyrifera_M_contig125490.5146.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig125490.5146.1
Unique NamemRNA_M-pyrifera_M_contig125490.5146.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig125490.5146.1 vs. uniprot
Match: UPI000644FBA1 (hypothetical protein n=1 Tax=Acytostelium subglobosum LB1 TaxID=1410327 RepID=UPI000644FBA1)

HSP 1 Score: 125 bits (315), Expect = 6.740e-32
Identity = 60/100 (60.00%), Postives = 73/100 (73.00%), Query Frame = 1 
Query:    1 VLVWGDLPAPRGGSAKTHPPAVHFNIFEGMEMHGIAEVTISRGKVVWRNNELIVEQGSGRYVPRPPFGMVFDTVKQRDEVRNELHRKVDREPYSGPVFVP 300
            +++W D  A +  SAKTH  AV FNIFEG+++ G+A  TI  GKVVW N  L    GSGR+VPRP  G VFD + +RDEVRNEL RKVDR+PY+GPVFVP
Sbjct:  399 IVIW-DPQAEKTISAKTHHHAVDFNIFEGIKVRGLAATTIVAGKVVWNNGTLSTTLGSGRFVPRPSHGCVFDGINKRDEVRNELLRKVDRQPYTGPVFVP 497
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BLAST of mRNA_M-pyrifera_M_contig125490.5146.1 vs. uniprot
Match: A0A7S4L6J3_9EUKA (Hypothetical protein n=1 Tax=Paramoeba aestuarina TaxID=180227 RepID=A0A7S4L6J3_9EUKA)

HSP 1 Score: 116 bits (290), Expect = 1.070e-31
Identity = 56/98 (57.14%), Postives = 75/98 (76.53%), Query Frame = 1 
Query:    4 LVW-GDLPAPRGGSAKTHPPAVHFNIFEGMEMHGIAEVTISRGKVVWRNNELIVEQGSGRYVPRPPFGMVFDTVKQRDEVRNELHRKVDREPYSGPVF 294
            +VW GD  A R  SAKTH  AV FNIFEGME+ GIA+VTISRG++VWR+ +L+ EQG G+YV R P+G ++D+V  RD+++    +KV+REPY+GPV 
Sbjct:    6 VVWNGD--ASRVISAKTHHHAVDFNIFEGMEVQGIADVTISRGRIVWRDGKLLTEQGWGKYVQRKPWGPIYDSVPIRDKLKERHQKKVEREPYTGPVI 101
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BLAST of mRNA_M-pyrifera_M_contig125490.5146.1 vs. uniprot
Match: A0A7S4LMK1_9EUGL (Hypothetical protein n=1 Tax=Eutreptiella gymnastica TaxID=73025 RepID=A0A7S4LMK1_9EUGL)

HSP 1 Score: 124 bits (311), Expect = 2.590e-31
Identity = 60/100 (60.00%), Postives = 72/100 (72.00%), Query Frame = 1 
Query:    1 VLVWGDLPAPRGGSAKTHPPAVHFNIFEGMEMHGIAEVTISRGKVVWRNNELIVEQGSGRYVPRPPFGMVFDTVKQRDEVRNELHRKVDREPYSGPVFVP 300
            +++W D  A R  SAKTH   V +NIFEGM++ GIA VTI+RGKV W N EL  EQG GR+VPR PFG VFD ++ RDE R+ L RKVDR PY GPV+ P
Sbjct:  402 IVIW-DGAAKRTISAKTHHHKVDYNIFEGMKIQGIAAVTITRGKVAWENGELKCEQGWGRFVPRKPFGPVFDGIETRDEARDLLKRKVDRPPYDGPVWTP 500
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BLAST of mRNA_M-pyrifera_M_contig125490.5146.1 vs. uniprot
Match: A0A8J4PN80_9MYCE (Amidohydro-rel domain-containing protein n=1 Tax=Polysphondylium violaceum TaxID=133409 RepID=A0A8J4PN80_9MYCE)

HSP 1 Score: 119 bits (297), Expect = 2.410e-29
Identity = 59/99 (59.60%), Postives = 70/99 (70.71%), Query Frame = 1 
Query:    1 VLVWGDLPAPRGGSAKTHPPAVHFNIFEGMEMHGIAEVTISRGKVVWRNNELIVEQGSGRYVPRPPFGMVFDTVKQRDEVRNELHRKVDREPYSGPVFV 297
            + VW D  A +  SA TH  AV FNIFEG+++ GIA  TI  G VVW NN L   +GSG++VPR PFG VFD +  RDE+RNEL RKVDREPY+GPV V
Sbjct:  399 IAVW-DPQATKTISASTHHHAVDFNIFEGIQVKGIATHTIVSGNVVWENNTLKTVKGSGKFVPRKPFGCVFDGIVTRDELRNELLRKVDREPYTGPVIV 496
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BLAST of mRNA_M-pyrifera_M_contig125490.5146.1 vs. uniprot
Match: A0A151Z489_9MYCE (Dihydropyrimidinase n=1 Tax=Tieghemostelium lacteum TaxID=361077 RepID=A0A151Z489_9MYCE)

HSP 1 Score: 118 bits (295), Expect = 4.480e-29
Identity = 57/100 (57.00%), Postives = 71/100 (71.00%), Query Frame = 1 
Query:    1 VLVWGDLPAPRGGSAKTHPPAVHFNIFEGMEMHGIAEVTISRGKVVWRNNELIVEQGSGRYVPRPPFGMVFDTVKQRDEVRNELHRKVDREPYSGPVFVP 300
            +++W D    +  S +TH  A  FNIFEGM + GIA  TI +GKVVW NN L   +GSG++VPR PFG VFD + QRD+VRNEL RKV+REPY+G VF P
Sbjct:  394 IVIW-DPKKTKTISKETHHHACDFNIFEGMNVTGIAVTTIVKGKVVWSNNTLSTVKGSGKFVPRLPFGSVFDGITQRDQVRNELLRKVEREPYTGKVFQP 492
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BLAST of mRNA_M-pyrifera_M_contig125490.5146.1 vs. uniprot
Match: A0A7S4L6I6_9EUKA (Hypothetical protein n=1 Tax=Paramoeba aestuarina TaxID=180227 RepID=A0A7S4L6I6_9EUKA)

HSP 1 Score: 117 bits (294), Expect = 6.400e-29
Identity = 56/99 (56.57%), Postives = 76/99 (76.77%), Query Frame = 1 
Query:    1 VLVW-GDLPAPRGGSAKTHPPAVHFNIFEGMEMHGIAEVTISRGKVVWRNNELIVEQGSGRYVPRPPFGMVFDTVKQRDEVRNELHRKVDREPYSGPVF 294
            ++VW GD  A R  SAKTH  AV FNIFEGME+ GIA+VTISRG++VWR+ +L+ EQG G+YV R P+G ++D+V  RD+++    +KV+REPY+GPV 
Sbjct:  400 IVVWNGD--ASRVISAKTHHHAVDFNIFEGMEVQGIADVTISRGRIVWRDGKLLTEQGWGKYVQRKPWGPIYDSVPIRDKLKERHQKKVEREPYTGPVI 496
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BLAST of mRNA_M-pyrifera_M_contig125490.5146.1 vs. uniprot
Match: D3AZB8_POLPP (Dihydropyrimidinase n=1 Tax=Polysphondylium pallidum (strain ATCC 26659 / Pp 5 / PN500) TaxID=670386 RepID=D3AZB8_POLPP)

HSP 1 Score: 118 bits (295), Expect = 1.040e-28
Identity = 56/96 (58.33%), Postives = 72/96 (75.00%), Query Frame = 1 
Query:    1 VLVWGDLPAPRGGSAKTHPPAVHFNIFEGMEMHGIAEVTISRGKVVWRNNELIVEQGSGRYVPRPPFGMVFDTVKQRDEVRNELHRKVDREPYSGP 288
            +++W D  A +  SAKTH  AV FNIFEGM++ G+A+ TI  GKVVW N++L   +GSG++VPRPPFGMVFD +  RD+VRNEL RKV+R PY  P
Sbjct:  759 IVLW-DPKATKTISAKTHHQAVDFNIFEGMKVTGLAQTTIVAGKVVWNNDKLSTVKGSGKFVPRPPFGMVFDGIDTRDKVRNELLRKVERAPYQPP 853
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BLAST of mRNA_M-pyrifera_M_contig125490.5146.1 vs. uniprot
Match: A0A2P6NT45_9EUKA (Dihydropyrimidinase n=1 Tax=Planoprotostelium fungivorum TaxID=1890364 RepID=A0A2P6NT45_9EUKA)

HSP 1 Score: 114 bits (284), Expect = 1.780e-27
Identity = 60/98 (61.22%), Postives = 74/98 (75.51%), Query Frame = 1 
Query:    1 VLVW-GDLPAPRGGSAKTHPPAVHFNIFEGMEMHGIAEVTISRGKVVWRNNELIVEQGSGRYVPRPPFGMVFDTVKQRDEVRNELHRKVDREPYSGPV 291
            V+VW GD  A R  SAKTH  AV FNIFEGM++HGIAEVTISRG+VV+R+  L   +GSGRY+ RP FG  FD ++ RD+ R+E   KV+REPY+GPV
Sbjct:  390 VVVWNGD--ASRVISAKTHHHAVDFNIFEGMKVHGIAEVTISRGRVVFRDGVLHTMKGSGRYIDRPCFGPPFDGIEARDKDRDERKLKVEREPYTGPV 485
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BLAST of mRNA_M-pyrifera_M_contig125490.5146.1 vs. uniprot
Match: L8GW65_ACACA (Dihydropyrimidinase n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8GW65_ACACA)

HSP 1 Score: 112 bits (280), Expect = 5.750e-27
Identity = 55/98 (56.12%), Postives = 68/98 (69.39%), Query Frame = 1 
Query:    1 VLVWGDLPAPRGGSAKTHPPAVHFNIFEGMEMHGIAEVTISRGKVVWRNNELIVEQGSGRYVPRPPFGMVFDTVKQRDEVRNELHRKVDREPYSGPVF 294
            +++W D  A R  SAKTH  AV FNIFEGME+ G+A  T+ RG+VVWR+ EL+  QG GRYVPRP +G  F +V QRD  R   H+ V REPY+GPV 
Sbjct:  395 IVIW-DGDAKRTISAKTHHHAVDFNIFEGMEVQGVAVTTLVRGRVVWRDGELVPAQGWGRYVPRPCWGPAFGSVAQRDAQRALYHQPVKREPYAGPVI 491
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BLAST of mRNA_M-pyrifera_M_contig125490.5146.1 vs. uniprot
Match: F0ZV30_DICPU (Dihydropyrimidinase n=1 Tax=Dictyostelium purpureum TaxID=5786 RepID=F0ZV30_DICPU)

HSP 1 Score: 112 bits (279), Expect = 8.060e-27
Identity = 54/98 (55.10%), Postives = 70/98 (71.43%), Query Frame = 1 
Query:    1 VLVWGDLPAPRGGSAKTHPPAVHFNIFEGMEMHGIAEVTISRGKVVWRNNELIVEQGSGRYVPRPPFGMVFDTVKQRDEVRNELHRKVDREPYSGPVF 294
            +++W D  A +  S  TH  AV FNIFEG+++ GIA  TI  G +VW +N+L  ++GSG +VPR PFG VF+ + QRD VRNEL RKVDREPY+GPV 
Sbjct:  396 IVLW-DPKASKTISKDTHHHAVDFNIFEGIKVTGIATTTIVAGNIVWNDNKLSTKKGSGTFVPRKPFGPVFNGIAQRDIVRNELLRKVDREPYTGPVI 492
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig125490.5146.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UPI000644FBA16.740e-3260.00hypothetical protein n=1 Tax=Acytostelium subglobo... [more]
A0A7S4L6J3_9EUKA1.070e-3157.14Hypothetical protein n=1 Tax=Paramoeba aestuarina ... [more]
A0A7S4LMK1_9EUGL2.590e-3160.00Hypothetical protein n=1 Tax=Eutreptiella gymnasti... [more]
A0A8J4PN80_9MYCE2.410e-2959.60Amidohydro-rel domain-containing protein n=1 Tax=P... [more]
A0A151Z489_9MYCE4.480e-2957.00Dihydropyrimidinase n=1 Tax=Tieghemostelium lacteu... [more]
A0A7S4L6I6_9EUKA6.400e-2956.57Hypothetical protein n=1 Tax=Paramoeba aestuarina ... [more]
D3AZB8_POLPP1.040e-2858.33Dihydropyrimidinase n=1 Tax=Polysphondylium pallid... [more]
A0A2P6NT45_9EUKA1.780e-2761.22Dihydropyrimidinase n=1 Tax=Planoprotostelium fung... [more]
L8GW65_ACACA5.750e-2756.12Dihydropyrimidinase n=1 Tax=Acanthamoeba castellan... [more]
F0ZV30_DICPU8.060e-2755.10Dihydropyrimidinase n=1 Tax=Dictyostelium purpureu... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig125490contigM-pyrifera_M_contig125490:188..499 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score111.7
Seed ortholog evalue2.1e-22
Seed eggNOG ortholog5786.XP_003291271.1
KEGG rclassRC00632,RC00680
KEGG koko:K01464,ko:K01465
KEGG ReactionR01993,R02269,R03055,R08227
KEGG Pathwayko00240,ko00410,ko00770,ko00983,ko01100,map00240,map00410,map00770,map00983,map01100
KEGG ModuleM00046,M00051
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0004157,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005783,GO:0005794,GO:0006139,GO:0006206,GO:0006208,GO:0006212,GO:0006725,GO:0006807,GO:0007154,GO:0008150,GO:0008152,GO:0008270,GO:0009056,GO:0009112,GO:0009605,GO:0009987,GO:0009991,GO:0012505,GO:0016787,GO:0016810,GO:0016812,GO:0017144,GO:0019860,GO:0031667,GO:0031668,GO:0031669,GO:0034641,GO:0042737,GO:0043167,GO:0043169,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043562,GO:0044237,GO:0044238,GO:0044248,GO:0044270,GO:0044281,GO:0044424,GO:0044444,GO:0044464,GO:0046113,GO:0046483,GO:0046700,GO:0046872,GO:0046914,GO:0050896,GO:0051716,GO:0055086,GO:0071496,GO:0071704,GO:0072527,GO:0072529,GO:1901360,GO:1901361,GO:1901564,GO:1901565,GO:1901575
EggNOG free text desc.Amidohydrolase family
EggNOG OGs3X84W@554915,COG0044@1,KOG2584@2759
EC3.5.2.2,3.5.2.3
COG Functional cat.F
Best tax levelAmoebozoa
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko04147
Exons1
Model size312
Cds size312
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815770.364885-CDS-M-pyrifera_M_contig125490:187..4991622815770.364885-CDS-M-pyrifera_M_contig125490:187..499Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig125490 188..499 -
1692277561.290156-CDS-M-pyrifera_M_contig125490:187..4991692277561.290156-CDS-M-pyrifera_M_contig125490:187..499Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig125490 188..499 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig125490.5146.1prot_M-pyrifera_M_contig125490.5146.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig125490 188..499 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig125490.5146.1

>prot_M-pyrifera_M_contig125490.5146.1 ID=prot_M-pyrifera_M_contig125490.5146.1|Name=mRNA_M-pyrifera_M_contig125490.5146.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=104bp
VLVWGDLPAPRGGSAKTHPPAVHFNIFEGMEMHGIAEVTISRGKVVWRNN
ELIVEQGSGRYVPRPPFGMVFDTVKQRDEVRNELHRKVDREPYSGPVFVP
GQD*
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mRNA from alignment at M-pyrifera_M_contig125490:188..499-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig125490.5146.1 ID=mRNA_M-pyrifera_M_contig125490.5146.1|Name=mRNA_M-pyrifera_M_contig125490.5146.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=312bp|location=Sequence derived from alignment at M-pyrifera_M_contig125490:188..499- (Macrocystis pyrifera P11B4 male)
GTCCTCGTCTGGGGGGACCTCCCGGCCCCGCGCGGCGGCTCCGCCAAGAC
GCACCCCCCCGCCGTCCACTTCAACATCTTCGAGGGCATGGAGATGCACG
GCATCGCCGAGGTCACGATCAGCCGCGGCAAGGTGGTGTGGCGCAACAAC
GAGCTCATCGTCGAGCAGGGCAGCGGCCGCTACGTGCCGCGCCCGCCCTT
CGGCATGGTCTTCGACACCGTCAAGCAGCGCGACGAGGTCAGGAACGAGC
TCCACCGCAAGGTCGACCGCGAGCCCTACAGCGGCCCCGTCTTCGTGCCC
GGCCAGGACTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig125490:188..499-

>mRNA_M-pyrifera_M_contig125490.5146.1 ID=mRNA_M-pyrifera_M_contig125490.5146.1|Name=mRNA_M-pyrifera_M_contig125490.5146.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=624bp|location=Sequence derived from alignment at M-pyrifera_M_contig125490:188..499- (Macrocystis pyrifera P11B4 male)
GTCCTCGTCTGGGGGGACCTCCCGGCCCCGCGCGGCGGCTCCGCCAAGAC
GCACCCCCCCGCCGTCCACTTCAACATCTTCGAGGGCATGGAGATGCACG
GCATCGCCGAGGTCACGATCAGCCGCGGCAAGGTGGTGTGGCGCAACAAC
GAGCTCATCGTCGAGCAGGGCAGCGGCCGCTACGTGCCGCGCCCGCCCTT
CGGCATGGTCTTCGACACCGTCAAGCAGCGCGACGAGGTCAGGAACGAGC
TCCACCGCAAGGTCGACCGCGAGCCCTACAGCGGCCCCGTCTTCGTGCCC
GGCCAGGACTGAGTCCTCGTCTGGGGGGACCTCCCGGCCCCGCGCGGCGG
CTCCGCCAAGACGCACCCCCCCGCCGTCCACTTCAACATCTTCGAGGGCA
TGGAGATGCACGGCATCGCCGAGGTCACGATCAGCCGCGGCAAGGTGGTG
TGGCGCAACAACGAGCTCATCGTCGAGCAGGGCAGCGGCCGCTACGTGCC
GCGCCCGCCCTTCGGCATGGTCTTCGACACCGTCAAGCAGCGCGACGAGG
TCAGGAACGAGCTCCACCGCAAGGTCGACCGCGAGCCCTACAGCGGCCCC
GTCTTCGTGCCCGGCCAGGACTGA
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