mRNA_M-pyrifera_M_contig125081.5086.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig125081.5086.1
Unique NamemRNA_M-pyrifera_M_contig125081.5086.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig125081.5086.1 vs. uniprot
Match: A0A6I6AC70_9PLAN (Glycoside hydrolase n=8 Tax=Gimesia TaxID=1649453 RepID=A0A6I6AC70_9PLAN)

HSP 1 Score: 355 bits (910), Expect = 2.960e-119
Identity = 171/171 (100.00%), Postives = 171/171 (100.00%), Query Frame = 1 
Query:    1 LTILHQRAQSLKLPVSLGCNLGTLIPDSSERAQICQAFDAVNVDLVNWKNIELYEGEQNWDQCDQQVDWCEKNNLLIRGGCLLDFAPEGLPDWLESWKLDMVNFQSIVSHFIETAITRYTGSIRNWTLVSAMNTGGVFGLNEETRLTLTARAIEVAKQTDDSIQCFIRVEQ 513
            LTILHQRAQSLKLPVSLGCNLGTLIPDSSERAQICQAFDAVNVDLVNWKNIELYEGEQNWDQCDQQVDWCEKNNLLIRGGCLLDFAPEGLPDWLESWKLDMVNFQSIVSHFIETAITRYTGSIRNWTLVSAMNTGGVFGLNEETRLTLTARAIEVAKQTDDSIQCFIRVEQ
Sbjct:  165 LTILHQRAQSLKLPVSLGCNLGTLIPDSSERAQICQAFDAVNVDLVNWKNIELYEGEQNWDQCDQQVDWCEKNNLLIRGGCLLDFAPEGLPDWLESWKLDMVNFQSIVSHFIETAITRYTGSIRNWTLVSAMNTGGVFGLNEETRLTLTARAIEVAKQTDDSIQCFIRVEQ 335
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BLAST of mRNA_M-pyrifera_M_contig125081.5086.1 vs. uniprot
Match: A0A517R9R1_9PLAN (GH10 domain-containing protein n=2 Tax=Gimesia TaxID=1649453 RepID=A0A517R9R1_9PLAN)

HSP 1 Score: 319 bits (817), Expect = 3.360e-105
Identity = 151/171 (88.30%), Postives = 159/171 (92.98%), Query Frame = 1 
Query:    1 LTILHQRAQSLKLPVSLGCNLGTLIPDSSERAQICQAFDAVNVDLVNWKNIELYEGEQNWDQCDQQVDWCEKNNLLIRGGCLLDFAPEGLPDWLESWKLDMVNFQSIVSHFIETAITRYTGSIRNWTLVSAMNTGGVFGLNEETRLTLTARAIEVAKQTDDSIQCFIRVEQ 513
            L ILHQRAQSLKLPVSLGCNLG  IP+S E  QICQAFDA N DL+NWK IE +EG+QNWD CDQQVDWCEKN+LLIRGGCLLDFAP+GLPDWLESWKLDMVNFQSI+SHFIETAITRYTGSIR WTLVSA+NTGGVFGLNEETRLTLTARAIEVAKQTDDSIQCFIRVEQ
Sbjct:  165 LMILHQRAQSLKLPVSLGCNLGEFIPNSQETRQICQAFDAANTDLINWKLIEQFEGDQNWDLCDQQVDWCEKNHLLIRGGCLLDFAPQGLPDWLESWKLDMVNFQSIISHFIETAITRYTGSIRTWTLVSAINTGGVFGLNEETRLTLTARAIEVAKQTDDSIQCFIRVEQ 335
[Close]
BLAST of mRNA_M-pyrifera_M_contig125081.5086.1 vs. uniprot
Match: A6C1A8_9PLAN (GH10 domain-containing protein n=9 Tax=Planctomycetaceae TaxID=126 RepID=A6C1A8_9PLAN)

HSP 1 Score: 317 bits (811), Expect = 2.700e-104
Identity = 148/171 (86.55%), Postives = 160/171 (93.57%), Query Frame = 1 
Query:    1 LTILHQRAQSLKLPVSLGCNLGTLIPDSSERAQICQAFDAVNVDLVNWKNIELYEGEQNWDQCDQQVDWCEKNNLLIRGGCLLDFAPEGLPDWLESWKLDMVNFQSIVSHFIETAITRYTGSIRNWTLVSAMNTGGVFGLNEETRLTLTARAIEVAKQTDDSIQCFIRVEQ 513
            LTILHQR+Q++KLPVSLGCNLG  IP++SE  QICQAFDA N+DLVNWK IE YEG+QNWD CDQQV+WCEK +LLIRGGCLLDFAP GLPDWLESWKLDMVNFQSI+SHFIETAITRYTGSIR WTLVSAMNTGGVFGLNEETRLTLTARAIE+AKQTDDSIQC+IRVEQ
Sbjct:  165 LTILHQRSQTMKLPVSLGCNLGEFIPNASEGKQICQAFDAANIDLVNWKLIEKYEGDQNWDLCDQQVEWCEKKHLLIRGGCLLDFAPHGLPDWLESWKLDMVNFQSIISHFIETAITRYTGSIRTWTLVSAMNTGGVFGLNEETRLTLTARAIEIAKQTDDSIQCYIRVEQ 335
[Close]
BLAST of mRNA_M-pyrifera_M_contig125081.5086.1 vs. uniprot
Match: A0A517W335_9PLAN (Glycosyl hydrolase family 10 n=2 Tax=Gimesia aquarii TaxID=2527964 RepID=A0A517W335_9PLAN)

HSP 1 Score: 303 bits (776), Expect = 6.090e-99
Identity = 142/171 (83.04%), Postives = 155/171 (90.64%), Query Frame = 1 
Query:    1 LTILHQRAQSLKLPVSLGCNLGTLIPDSSERAQICQAFDAVNVDLVNWKNIELYEGEQNWDQCDQQVDWCEKNNLLIRGGCLLDFAPEGLPDWLESWKLDMVNFQSIVSHFIETAITRYTGSIRNWTLVSAMNTGGVFGLNEETRLTLTARAIEVAKQTDDSIQCFIRVEQ 513
            L ILHQR+Q++KLPVSLGCNLG  IP+  E   ICQAFDA N DL+NWK IE  EG+QNW+ CDQQVDWCEKN+LLIRGGCLLDFAP GLPDWLESWKLDMVNFQSI+SHFIETAITRY+GSIR WT+VS+MNTGGVFGLNEETRLTLTARAIEVAK+TDDSIQCFIRVEQ
Sbjct:  171 LAILHQRSQAMKLPVSLGCNLGEFIPNKQESQLICQAFDAANTDLINWKLIEPIEGDQNWELCDQQVDWCEKNHLLIRGGCLLDFAPHGLPDWLESWKLDMVNFQSIISHFIETAITRYSGSIRIWTMVSSMNTGGVFGLNEETRLTLTARAIEVAKKTDDSIQCFIRVEQ 341
[Close]
BLAST of mRNA_M-pyrifera_M_contig125081.5086.1 vs. uniprot
Match: A0A2E7CYA3_9PLAN (Glycoside hydrolase n=1 Tax=Planctomycetaceae bacterium TaxID=2026779 RepID=A0A2E7CYA3_9PLAN)

HSP 1 Score: 180 bits (457), Expect = 2.080e-51
Identity = 84/166 (50.60%), Postives = 113/166 (68.07%), Query Frame = 1 
Query:   16 QRAQSLKLPVSLGCNLGTLIPDSSERAQICQAFDAVNVDLVNWKNIELYEGEQNWDQCDQQVDWCEKNNLLIRGGCLLDFAPEGLPDWLESWKLDMVNFQSIVSHFIETAITRYTGSIRNWTLVSAMNTGGVFGLNEETRLTLTARAIEVAKQTDDSIQCFIRVEQ 513
            +R +S +LP SLGCNLG  +P +   +QIC  F A ++  V+WK++E  EGE  W+  D+QVDWC +N +L+RGG LLD +P GLP WL  W+ D  N QS +  ++ETAI+RY G IR W + S +NTGG   LNEE RLTL AR +EVA+Q D+  +  IRV+Q
Sbjct:  168 RRHRSQQLPASLGCNLGNTVPGNEWPSQICTKFTAASIP-VSWKHVEPAEGEYFWELYDEQVDWCTENKMLMRGGPLLDMSPGGLPAWLSQWEHDFFNLQSFLCDYVETAISRYLGKIRTWEISSRVNTGGALQLNEENRLTLVARTLEVARQVDEEAKLLIRVDQ 332
[Close]
BLAST of mRNA_M-pyrifera_M_contig125081.5086.1 vs. uniprot
Match: UPI0014218C27 (endo-1,4-beta-xylanase n=1 Tax=Rubinisphaera sp. JC658 TaxID=2713176 RepID=UPI0014218C27)

HSP 1 Score: 168 bits (425), Expect = 9.810e-47
Identity = 82/166 (49.40%), Postives = 106/166 (63.86%), Query Frame = 1 
Query:   16 QRAQSLKLPVSLGCNLGTLIPDSSERAQICQAFDAVNVDLVNWKNIELYEGEQNWDQCDQQVDWCEKNNLLIRGGCLLDFAPEGLPDWLESWKLDMVNFQSIVSHFIETAITRYTGSIRNWTLVSAMNTGGVFGLNEETRLTLTARAIEVAKQTDDSIQCFIRVEQ 513
            +RA++  LP SLGC LG   P+         AF+A  V  + W+ IE  EG+  WD  D QV+WCEKN LL+  G LLD +PEGLPDWL  W+ +++N QS VS F+ETAITRY G IR W + +  NTGG   L+EE RL+L AR +E+A Q D   Q  +RV+Q
Sbjct:  165 RRARTTHLPASLGCGLGHAPPNPDWEDSFVNAFNAALVP-IEWRFIEPEEGKYEWDTNDAQVEWCEKNRLLMTAGPLLDLSPEGLPDWLWQWQHEVLNLQSFVSDFVETAITRYAGRIRFWEVAARANTGGALALSEEDRLSLAARTLEIASQVDQDNQLMVRVDQ 329
[Close]
BLAST of mRNA_M-pyrifera_M_contig125081.5086.1 vs. uniprot
Match: UPI00029B433A (endo-1,4-beta-xylanase n=1 Tax=Schlesneria paludicola TaxID=360056 RepID=UPI00029B433A)

HSP 1 Score: 167 bits (422), Expect = 2.990e-46
Identity = 78/159 (49.06%), Postives = 101/159 (63.52%), Query Frame = 1 
Query:   37 LPVSLGCNLGTLIPDSSERAQICQAFDAVNVDLVNWKNIELYEGEQNWDQCDQQVDWCEKNNLLIRGGCLLDFAPEGLPDWLESWKLDMVNFQSIVSHFIETAITRYTGSIRNWTLVSAMNTGGVFGLNEETRLTLTARAIEVAKQTDDSIQCFIRVEQ 513
            LP+S+GC +   +P  +        F+   V  V WK IE  EGE NW+  D+Q+DWCE   L++RGG LLD     +P WL  W+ D++N QS V  F+ETAI RY G IR W + S MNTGG  GLNEETRLTLTAR ++VA+Q D+  Q  IR++Q
Sbjct:  175 LPISIGCEILGTLPPPATHVLYGTLFNTSTVS-VPWKVIEAIEGEYNWEATDKQLDWCESQKLMVRGGPLLDLGSNSMPGWLSRWEHDVLNLQSFVCDFVETAIARYVGRIRVWEICSRMNTGGAMGLNEETRLTLTARVLDVARQVDEEAQLIIRIDQ 332
[Close]
BLAST of mRNA_M-pyrifera_M_contig125081.5086.1 vs. uniprot
Match: UPI00199301F5 (Endo-1,4-beta-xylanase n=1 Tax=Candidatus Saccharimonas sp. TaxID=1917697 RepID=UPI00199301F5)

HSP 1 Score: 165 bits (418), Expect = 1.130e-45
Identity = 74/159 (46.54%), Postives = 101/159 (63.52%), Query Frame = 1 
Query:   37 LPVSLGCNLGTLIPDSSERAQICQAFDAVNVDLVNWKNIELYEGEQNWDQCDQQVDWCEKNNLLIRGGCLLDFAPEGLPDWLESWKLDMVNFQSIVSHFIETAITRYTGSIRNWTLVSAMNTGGVFGLNEETRLTLTARAIEVAKQTDDSIQCFIRVEQ 513
            LP S+GC L    P          AF++  V  V W+ IE  EGE +W+ CD+Q++WCE+  L++RGG L+D  P+GLP WL  W+ D+ N QS V  F+ETAI+RY G IR W + + +NTGG   L EE RLTL AR ++VA+Q D+  Q  IR++Q
Sbjct:  175 LPASIGCELQGSTPKPEANELFAAAFNSATV-AVPWRKIEAVEGEYDWEPCDRQLEWCEQQKLMVRGGPLIDLGPDGLPPWLARWEHDIFNLQSFVCDFVETAISRYVGRIRIWEVAARLNTGGALSLTEENRLTLAARVLDVARQVDEEAQLLIRIDQ 332
[Close]
BLAST of mRNA_M-pyrifera_M_contig125081.5086.1 vs. uniprot
Match: A0A3L7PE83_9BACT (Glycoside hydrolase n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A3L7PE83_9BACT)

HSP 1 Score: 162 bits (411), Expect = 1.190e-44
Identity = 76/166 (45.78%), Postives = 103/166 (62.05%), Query Frame = 1 
Query:   16 QRAQSLKLPVSLGCNLGTLIPDSSERAQICQAFDAVNVDLVNWKNIELYEGEQNWDQCDQQVDWCEKNNLLIRGGCLLDFAPEGLPDWLESWKLDMVNFQSIVSHFIETAITRYTGSIRNWTLVSAMNTGGVFGLNEETRLTLTARAIEVAKQTDDSIQCFIRVEQ 513
            QR Q L  P S+GC L   IP          AF++  V +  W  IE+ EGE NW+ CD+Q++WCE+  L++RGG LLD  P GLP WL  W+ D+ N QS V  F+ETAI+RY G IR W + + +N+GG   L E + LTL AR ++VA+Q D+  Q  IR++Q
Sbjct:  170 QRFQGL--PASIGCELQGSIPSPDANELFSAAFNSATVSIP-WNKIEVVEGEYNWEPCDRQLEWCEQQKLMVRGGSLLDLGPGGLPTWLARWEHDIFNLQSFVCDFVETAISRYVGRIRLWEIAARLNSGGALALTEASLLTLAARVLDVARQVDEEAQLLIRIDQ 332
[Close]
BLAST of mRNA_M-pyrifera_M_contig125081.5086.1 vs. uniprot
Match: F0SMP4_RUBBR (Glycoside hydrolase family 10 n=2 Tax=Rubinisphaera TaxID=1649490 RepID=F0SMP4_RUBBR)

HSP 1 Score: 160 bits (405), Expect = 8.210e-44
Identity = 76/158 (48.10%), Postives = 105/158 (66.46%), Query Frame = 1 
Query:   40 PVSLGCNLGTLIPDSSERAQICQAFDAVNVDLVNWKNIELYEGEQNWDQCDQQVDWCEKNNLLIRGGCLLDFAPEGLPDWLESWKLDMVNFQSIVSHFIETAITRYTGSIRNWTLVSAMNTGGVFGLNEETRLTLTARAIEVAKQTDDSIQCFIRVEQ 513
            P   GC+LG ++P+          F+A  V L +WK IE  EGE +WD  DQQVDW  +N ++++GG LL F    LP+WLE+WK D++N QS +S ++ETA++RYTG IR W + + MNTGG+FG  EE  L LTAR I+VA+Q D   Q ++RV+Q
Sbjct:  176 PALFGCDLGPVLPNQLPE-NFTDTFNAAAVTL-DWKAIESVEGEYSWDVYDQQVDWALRNKVMVKGGPLLGFQEGDLPNWLENWKHDILNLQSFLSDYVETAVSRYTGKIRMWEVAARMNTGGLFGYTEENLLALTARMIDVARQVDGESQIYVRVDQ 331
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig125081.5086.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6I6AC70_9PLAN2.960e-119100.00Glycoside hydrolase n=8 Tax=Gimesia TaxID=1649453 ... [more]
A0A517R9R1_9PLAN3.360e-10588.30GH10 domain-containing protein n=2 Tax=Gimesia Tax... [more]
A6C1A8_9PLAN2.700e-10486.55GH10 domain-containing protein n=9 Tax=Planctomyce... [more]
A0A517W335_9PLAN6.090e-9983.04Glycosyl hydrolase family 10 n=2 Tax=Gimesia aquar... [more]
A0A2E7CYA3_9PLAN2.080e-5150.60Glycoside hydrolase n=1 Tax=Planctomycetaceae bact... [more]
UPI0014218C279.810e-4749.40endo-1,4-beta-xylanase n=1 Tax=Rubinisphaera sp. J... [more]
UPI00029B433A2.990e-4649.06endo-1,4-beta-xylanase n=1 Tax=Schlesneria paludic... [more]
UPI00199301F51.130e-4546.54Endo-1,4-beta-xylanase n=1 Tax=Candidatus Sacchari... [more]
A0A3L7PE83_9BACT1.190e-4445.78Glycoside hydrolase n=1 Tax=Planctomycetes bacteri... [more]
F0SMP4_RUBBR8.210e-4448.10Glycoside hydrolase family 10 n=2 Tax=Rubinisphaer... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig125081contigM-pyrifera_M_contig125081:2..514 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score319.3
Seed ortholog evalue1.1e-84
Seed eggNOG ortholog344747.PM8797T_06000
Hectar predicted targeting categoryother localisation
EggNOG free text desc.PFAM glycoside hydrolase, family 10
EggNOG OGs2IXI6@203682,COG3693@1,COG3693@2
COG Functional cat.G
Best tax levelPlanctomycetes
Best eggNOG OGNA|NA|NA
Exons1
Model size513
Cds size513
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815767.1069005-CDS-M-pyrifera_M_contig125081:1..5141622815767.1069005-CDS-M-pyrifera_M_contig125081:1..514Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig125081 2..514 -
1692277559.8161833-CDS-M-pyrifera_M_contig125081:1..5141692277559.8161833-CDS-M-pyrifera_M_contig125081:1..514Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig125081 2..514 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig125081.5086.1prot_M-pyrifera_M_contig125081.5086.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig125081 2..514 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig125081.5086.1

>prot_M-pyrifera_M_contig125081.5086.1 ID=prot_M-pyrifera_M_contig125081.5086.1|Name=mRNA_M-pyrifera_M_contig125081.5086.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=171bp
LTILHQRAQSLKLPVSLGCNLGTLIPDSSERAQICQAFDAVNVDLVNWKN
IELYEGEQNWDQCDQQVDWCEKNNLLIRGGCLLDFAPEGLPDWLESWKLD
MVNFQSIVSHFIETAITRYTGSIRNWTLVSAMNTGGVFGLNEETRLTLTA
RAIEVAKQTDDSIQCFIRVEQ
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mRNA from alignment at M-pyrifera_M_contig125081:2..514-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig125081.5086.1 ID=mRNA_M-pyrifera_M_contig125081.5086.1|Name=mRNA_M-pyrifera_M_contig125081.5086.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=513bp|location=Sequence derived from alignment at M-pyrifera_M_contig125081:2..514- (Macrocystis pyrifera P11B4 male)
CTGACGATTCTGCATCAGCGGGCACAGTCGTTGAAACTGCCCGTTTCCCT
GGGCTGTAACCTGGGAACCCTGATTCCTGACAGTTCCGAACGGGCTCAGA
TCTGCCAGGCCTTTGATGCCGTCAACGTGGATCTGGTGAACTGGAAAAAC
ATCGAACTCTACGAAGGCGAGCAAAACTGGGACCAGTGCGATCAGCAGGT
TGACTGGTGCGAAAAAAACAATCTGCTGATTCGCGGAGGCTGCCTGCTCG
ATTTCGCCCCGGAAGGGCTTCCCGACTGGCTTGAGTCCTGGAAGCTGGAT
ATGGTCAACTTCCAGAGCATCGTTTCCCATTTCATTGAGACCGCGATCAC
CCGGTATACGGGTAGTATTCGAAACTGGACGCTGGTTTCTGCCATGAACA
CCGGCGGCGTCTTCGGACTCAATGAAGAGACCCGGCTCACTCTCACCGCC
CGCGCCATCGAAGTTGCTAAACAGACGGATGATTCCATCCAGTGTTTCAT
CAGAGTGGAACAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig125081:2..514-

>mRNA_M-pyrifera_M_contig125081.5086.1 ID=mRNA_M-pyrifera_M_contig125081.5086.1|Name=mRNA_M-pyrifera_M_contig125081.5086.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1026bp|location=Sequence derived from alignment at M-pyrifera_M_contig125081:2..514- (Macrocystis pyrifera P11B4 male)
CTGACGATTCTGCATCAGCGGGCACAGTCGTTGAAACTGCCCGTTTCCCT
GGGCTGTAACCTGGGAACCCTGATTCCTGACAGTTCCGAACGGGCTCAGA
TCTGCCAGGCCTTTGATGCCGTCAACGTGGATCTGGTGAACTGGAAAAAC
ATCGAACTCTACGAAGGCGAGCAAAACTGGGACCAGTGCGATCAGCAGGT
TGACTGGTGCGAAAAAAACAATCTGCTGATTCGCGGAGGCTGCCTGCTCG
ATTTCGCCCCGGAAGGGCTTCCCGACTGGCTTGAGTCCTGGAAGCTGGAT
ATGGTCAACTTCCAGAGCATCGTTTCCCATTTCATTGAGACCGCGATCAC
CCGGTATACGGGTAGTATTCGAAACTGGACGCTGGTTTCTGCCATGAACA
CCGGCGGCGTCTTCGGACTCAATGAAGAGACCCGGCTCACTCTCACCGCC
CGCGCCATCGAAGTTGCTAAACAGACGGATGATTCCATCCAGTGTTTCAT
CAGAGTGGAACAGCTGACGATTCTGCATCAGCGGGCACAGTCGTTGAAAC
TGCCCGTTTCCCTGGGCTGTAACCTGGGAACCCTGATTCCTGACAGTTCC
GAACGGGCTCAGATCTGCCAGGCCTTTGATGCCGTCAACGTGGATCTGGT
GAACTGGAAAAACATCGAACTCTACGAAGGCGAGCAAAACTGGGACCAGT
GCGATCAGCAGGTTGACTGGTGCGAAAAAAACAATCTGCTGATTCGCGGA
GGCTGCCTGCTCGATTTCGCCCCGGAAGGGCTTCCCGACTGGCTTGAGTC
CTGGAAGCTGGATATGGTCAACTTCCAGAGCATCGTTTCCCATTTCATTG
AGACCGCGATCACCCGGTATACGGGTAGTATTCGAAACTGGACGCTGGTT
TCTGCCATGAACACCGGCGGCGTCTTCGGACTCAATGAAGAGACCCGGCT
CACTCTCACCGCCCGCGCCATCGAAGTTGCTAAACAGACGGATGATTCCA
TCCAGTGTTTCATCAGAGTGGAACAG
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