mRNA_M-pyrifera_M_contig123014.4732.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig123014.4732.1
Unique NamemRNA_M-pyrifera_M_contig123014.4732.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig123014.4732.1 vs. uniprot
Match: A0A3D5DGY1_9GAMM (Mannose-1-phosphate guanylyltransferase n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A3D5DGY1_9GAMM)

HSP 1 Score: 266 bits (679), Expect = 7.520e-85
Identity = 125/155 (80.65%), Postives = 136/155 (87.74%), Query Frame = 1 
Query:    7 LGCEDLVIVETPDAVMVAKKDRAQDVKKIVDWLNEQGREERLFHRRVYRPWGSYEGVDAGTRFQVKRIVVNPGASLSLQMHRHRAEHWIVVSGTAKVTCAEREFLLSENESTYIPIGATHRLENPGTIPCELIEVQSGSYLGEDDIVRFEDKYNR 471
            LGCEDLV+VET DAVMV KKDR QDVKKIVDWL EQGR+ERL HRRVYRPWGSYE +D GT FQVKRI+VNP ASLSLQMH HRAEHW+VVSGTA VTC + +F LSENEST+IPIG THRLEN G  PCELIEVQSG+YLGEDDIVRF+D Y+R
Sbjct:  327 LGCEDLVVVETSDAVMVTKKDRTQDVKKIVDWLEEQGRDERLVHRRVYRPWGSYERIDIGTGFQVKRIIVNPSASLSLQMHHHRAEHWVVVSGTATVTCGDEKFTLSENESTFIPIGVTHRLENSGASPCELIEVQSGTYLGEDDIVRFDDIYDR 481
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BLAST of mRNA_M-pyrifera_M_contig123014.4732.1 vs. uniprot
Match: A0A3B1B373_9ZZZZ (Mannose-1-phosphate guanylyltransferase n=1 Tax=hydrothermal vent metagenome TaxID=652676 RepID=A0A3B1B373_9ZZZZ)

HSP 1 Score: 259 bits (663), Expect = 2.360e-82
Identity = 123/157 (78.34%), Postives = 138/157 (87.90%), Query Frame = 1 
Query:    1 AGLGCEDLVIVETPDAVMVAKKDRAQDVKKIVDWLNEQGREERLFHRRVYRPWGSYEGVDAGTRFQVKRIVVNPGASLSLQMHRHRAEHWIVVSGTAKVTCAEREFLLSENESTYIPIGATHRLENPGTIPCELIEVQSGSYLGEDDIVRFEDKYNR 471
            AG+G +D+++VET DAVMVA KD AQDVKKIVD L    R ERL HR+VYRPWGSYEG+D+G RFQVKRIVVNPGA+LSLQMH HRAEHWIVV GTAKVTC ++EFLLSEN+STYIPIG  HRLENPGT+P E+IEVQSGSYLGEDDIVRFED+Y R
Sbjct:  325 AGVGLKDILVVETADAVMVAHKDHAQDVKKIVDQLKADQRSERLVHRQVYRPWGSYEGIDSGERFQVKRIVVNPGAALSLQMHHHRAEHWIVVKGTAKVTCGDKEFLLSENQSTYIPIGEKHRLENPGTMPLEIIEVQSGSYLGEDDIVRFEDRYGR 481
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BLAST of mRNA_M-pyrifera_M_contig123014.4732.1 vs. uniprot
Match: A0A2A4Y634_9GAMM (Mannose-1-phosphate guanylyltransferase n=1 Tax=Ectothiorhodospiraceae bacterium TaxID=2026737 RepID=A0A2A4Y634_9GAMM)

HSP 1 Score: 259 bits (662), Expect = 5.200e-82
Identity = 122/158 (77.22%), Postives = 139/158 (87.97%), Query Frame = 1 
Query:    1 AGLGCEDLVIVETPDAVMVAKKDRAQDVKKIVDWLNEQGREERLFHRRVYRPWGSYEGVDAGTRFQVKRIVVNPGASLSLQMHRHRAEHWIVVSGTAKVTCAEREFLLSENESTYIPIGATHRLENPGTIPCELIEVQSGSYLGEDDIVRFEDKYNRA 474
            AG+G +++V+VET DAVMVA KD AQDVKKIV+ L   GR ERL HR+VYRPWGSYEGVDAG RFQVKRI+VNPGA+LSLQMH HRAEHWIVV GTA+VTC E+EFLLSEN+ST+IPIG  HRLENPG++P E+IEVQSGSYLGEDDIVRFED+Y R 
Sbjct:  346 AGVGLDNIVVVETADAVMVAHKDHAQDVKKIVERLKADGRSERLVHRQVYRPWGSYEGVDAGERFQVKRIIVNPGAALSLQMHHHRAEHWIVVKGTARVTCGEKEFLLSENQSTFIPIGEKHRLENPGSLPLEIIEVQSGSYLGEDDIVRFEDRYGRG 503
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BLAST of mRNA_M-pyrifera_M_contig123014.4732.1 vs. uniprot
Match: A0A3S8ZTK6_9NEIS (Mannose-1-phosphate guanylyltransferase n=6 Tax=Betaproteobacteria TaxID=28216 RepID=A0A3S8ZTK6_9NEIS)

HSP 1 Score: 253 bits (645), Expect = 7.410e-80
Identity = 122/158 (77.22%), Postives = 136/158 (86.08%), Query Frame = 1 
Query:    1 AGLGCEDLVIVETPDAVMVAKKDRAQDVKKIVDWLNEQGREERLFHRRVYRPWGSYEGVDAGTRFQVKRIVVNPGASLSLQMHRHRAEHWIVVSGTAKVTCAEREFLLSENESTYIPIGATHRLENPGTIPCELIEVQSGSYLGEDDIVRFEDKYNRA 474
            A +G +DLVIVET DAV+VA KDR QDVKKIV+ LN+ GR E + HRRVYRPWGSYEG+DAG+RFQVKRIVVNPGASLSLQMH HRAEHWIVV GTA+V   ++E LLSEN+STYIP+G THRLENPG +P ELIEVQSGSYLGEDDIVRFED Y R 
Sbjct:  314 AAIGVQDLVIVETADAVLVAHKDRVQDVKKIVERLNQAGRSESVTHRRVYRPWGSYEGIDAGSRFQVKRIVVNPGASLSLQMHYHRAEHWIVVKGTARVVNGDQEILLSENQSTYIPLGTTHRLENPGKMPLELIEVQSGSYLGEDDIVRFEDVYGRT 471
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BLAST of mRNA_M-pyrifera_M_contig123014.4732.1 vs. uniprot
Match: UPI001E2C6A83 (mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase n=1 Tax=Burkholderia diffusa TaxID=488732 RepID=UPI001E2C6A83)

HSP 1 Score: 251 bits (641), Expect = 2.520e-79
Identity = 118/158 (74.68%), Postives = 135/158 (85.44%), Query Frame = 1 
Query:    1 AGLGCEDLVIVETPDAVMVAKKDRAQDVKKIVDWLNEQGREERLFHRRVYRPWGSYEGVDAGTRFQVKRIVVNPGASLSLQMHRHRAEHWIVVSGTAKVTCAEREFLLSENESTYIPIGATHRLENPGTIPCELIEVQSGSYLGEDDIVRFEDKYNRA 474
            A +G +D+VIVET DAV+VA +D+AQDVKKIV+WLNE GR E + HRRV RPWGSYEG+D G RFQVKRI+VNPGA LSLQMH HRAEHWIVV GTA VT  ++E +L+EN+STYIP+GATHRL NPG IP ELIEVQSG+YLGEDDIVRFED Y RA
Sbjct:  307 AAIGLDDIVIVETADAVLVAHRDKAQDVKKIVEWLNESGRHESVTHRRVIRPWGSYEGIDQGDRFQVKRIIVNPGAQLSLQMHHHRAEHWIVVKGTALVTNGDKEIMLTENQSTYIPLGATHRLTNPGKIPLELIEVQSGAYLGEDDIVRFEDTYGRA 464
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BLAST of mRNA_M-pyrifera_M_contig123014.4732.1 vs. uniprot
Match: A0A351MEF8_9GAMM (Mannose-1-phosphate guanylyltransferase n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A351MEF8_9GAMM)

HSP 1 Score: 251 bits (640), Expect = 4.640e-79
Identity = 118/158 (74.68%), Postives = 135/158 (85.44%), Query Frame = 1 
Query:    1 AGLGCEDLVIVETPDAVMVAKKDRAQDVKKIVDWLNEQGREERLFHRRVYRPWGSYEGVDAGTRFQVKRIVVNPGASLSLQMHRHRAEHWIVVSGTAKVTCAEREFLLSENESTYIPIGATHRLENPGTIPCELIEVQSGSYLGEDDIVRFEDKYNRA 474
            A LGC+D+V+VET DAV+VA K RAQ+VKK+V+WL  +GR+ERL HR VYRPWGSYEG+D G RFQVKRI V PG  LSLQMH HRAEHWIVVSGTA+VT  +  FLL+EN+ST+IP+G  HRLENPGTIP ELIEVQSGSYLGEDDIVRF+D YNRA
Sbjct:  319 ATLGCDDMVVVETADAVLVAPKARAQEVKKVVEWLKSRGRDERLTHRCVYRPWGSYEGIDIGDRFQVKRITVKPGGKLSLQMHHHRAEHWIVVSGTAQVTKGDEVFLLTENQSTFIPLGVRHRLENPGTIPLELIEVQSGSYLGEDDIVRFQDVYNRA 476
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BLAST of mRNA_M-pyrifera_M_contig123014.4732.1 vs. uniprot
Match: UPI0012909F3C (mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase n=1 Tax=Propionivibrio limicola TaxID=167645 RepID=UPI0012909F3C)

HSP 1 Score: 249 bits (635), Expect = 2.290e-78
Identity = 118/158 (74.68%), Postives = 134/158 (84.81%), Query Frame = 1 
Query:    1 AGLGCEDLVIVETPDAVMVAKKDRAQDVKKIVDWLNEQGREERLFHRRVYRPWGSYEGVDAGTRFQVKRIVVNPGASLSLQMHRHRAEHWIVVSGTAKVTCAEREFLLSENESTYIPIGATHRLENPGTIPCELIEVQSGSYLGEDDIVRFEDKYNRA 474
            A +G E+LVIVET DA +V  KD AQDVKKIV+ LN  GR E +FHRRV+RPWGSYEG+DAG RFQVKRI+VNPGA+LSLQMH HRAEHWIVV GTAKVT   +E LL+EN+STYIP+G THRLENPG +P ELIEVQSGSYLGEDDIVRF+D+Y R 
Sbjct:  313 AAIGVENLVIVETADATLVTTKDHAQDVKKIVERLNRDGRGETMFHRRVFRPWGSYEGIDAGDRFQVKRIIVNPGATLSLQMHYHRAEHWIVVKGTAKVTNGSKEILLAENQSTYIPLGETHRLENPGKVPLELIEVQSGSYLGEDDIVRFDDQYGRT 470
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BLAST of mRNA_M-pyrifera_M_contig123014.4732.1 vs. uniprot
Match: B1JWE1_BURCC (Mannose-1-phosphate guanylyltransferase n=11 Tax=Burkholderia TaxID=32008 RepID=B1JWE1_BURCC)

HSP 1 Score: 248 bits (633), Expect = 4.950e-78
Identity = 117/158 (74.05%), Postives = 134/158 (84.81%), Query Frame = 1 
Query:    1 AGLGCEDLVIVETPDAVMVAKKDRAQDVKKIVDWLNEQGREERLFHRRVYRPWGSYEGVDAGTRFQVKRIVVNPGASLSLQMHRHRAEHWIVVSGTAKVTCAEREFLLSENESTYIPIGATHRLENPGTIPCELIEVQSGSYLGEDDIVRFEDKYNRA 474
            A LG +++VIVET DAV+VA +D+AQDVKKIV+WLN  GR E + HRRV RPWGSYEG+D G RFQVKRI+VNPGA LSLQMH HRAEHWIVV GTA VT  ++E +L+EN+STYIP+GATHRL NPG IP ELIEVQSG+YLGEDDIVRFED Y RA
Sbjct:  315 AALGLDNIVIVETADAVLVAHRDKAQDVKKIVEWLNASGRHESVTHRRVVRPWGSYEGIDQGDRFQVKRIIVNPGAQLSLQMHHHRAEHWIVVKGTAMVTNGDKEIMLTENQSTYIPLGATHRLTNPGKIPLELIEVQSGAYLGEDDIVRFEDTYGRA 472
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BLAST of mRNA_M-pyrifera_M_contig123014.4732.1 vs. uniprot
Match: A0A0X3U6G1_9GAMM (Mannose-1-phosphate guanylyltransferase n=1 Tax=Microbulbifer sp. ZGT114 TaxID=1685377 RepID=A0A0X3U6G1_9GAMM)

HSP 1 Score: 248 bits (632), Expect = 6.630e-78
Identity = 116/158 (73.42%), Postives = 134/158 (84.81%), Query Frame = 1 
Query:    1 AGLGCEDLVIVETPDAVMVAKKDRAQDVKKIVDWLNEQGREERLFHRRVYRPWGSYEGVDAGTRFQVKRIVVNPGASLSLQMHRHRAEHWIVVSGTAKVTCAEREFLLSENESTYIPIGATHRLENPGTIPCELIEVQSGSYLGEDDIVRFEDKYNRA 474
            A +G EDLV+VE+ DA+MVA KDR QDVKK+V+ L  + R E + HR+VYRPWG Y+ VDAG RFQVKRIVVNPGA LSLQMH HRAEHW+VVSGTA+VTC + + LLSEN+STYIP+G  HRLENPG IP ELIEVQSGSYLGEDDIVRFEDKY+R+
Sbjct:  315 AAIGLEDLVVVESDDAIMVAAKDRVQDVKKVVERLKAENRPEAMVHRKVYRPWGFYDSVDAGERFQVKRIVVNPGAQLSLQMHHHRAEHWVVVSGTARVTCGDEQRLLSENQSTYIPLGVKHRLENPGAIPLELIEVQSGSYLGEDDIVRFEDKYSRS 472
[Close]
BLAST of mRNA_M-pyrifera_M_contig123014.4732.1 vs. uniprot
Match: A0A6J5J733_9BURK (Mannose-1-phosphate guanylyltransferase n=89 Tax=Burkholderiaceae TaxID=119060 RepID=A0A6J5J733_9BURK)

HSP 1 Score: 247 bits (631), Expect = 1.010e-77
Identity = 116/158 (73.42%), Postives = 133/158 (84.18%), Query Frame = 1 
Query:    1 AGLGCEDLVIVETPDAVMVAKKDRAQDVKKIVDWLNEQGREERLFHRRVYRPWGSYEGVDAGTRFQVKRIVVNPGASLSLQMHRHRAEHWIVVSGTAKVTCAEREFLLSENESTYIPIGATHRLENPGTIPCELIEVQSGSYLGEDDIVRFEDKYNRA 474
            A +G +D+VIVET DAV+VA +D+AQDVKKIV+WLN  GR E + HR V RPWGSYEG+D G RFQVKRI+VNPGA LSLQMH HRAEHWIVV GTA VT  ++E +L+EN+STYIP+GATHRL NPG IP ELIEVQSG+YLGEDDIVRFED Y RA
Sbjct:  316 AAIGLDDIVIVETADAVLVAHRDKAQDVKKIVEWLNASGRHESVTHRHVVRPWGSYEGIDQGDRFQVKRIIVNPGAQLSLQMHHHRAEHWIVVKGTAMVTNGDKELMLTENQSTYIPLGATHRLTNPGKIPLELIEVQSGAYLGEDDIVRFEDTYGRA 473
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig123014.4732.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A3D5DGY1_9GAMM7.520e-8580.65Mannose-1-phosphate guanylyltransferase n=1 Tax=Ga... [more]
A0A3B1B373_9ZZZZ2.360e-8278.34Mannose-1-phosphate guanylyltransferase n=1 Tax=hy... [more]
A0A2A4Y634_9GAMM5.200e-8277.22Mannose-1-phosphate guanylyltransferase n=1 Tax=Ec... [more]
A0A3S8ZTK6_9NEIS7.410e-8077.22Mannose-1-phosphate guanylyltransferase n=6 Tax=Be... [more]
UPI001E2C6A832.520e-7974.68mannose-1-phosphate guanylyltransferase/mannose-6-... [more]
A0A351MEF8_9GAMM4.640e-7974.68Mannose-1-phosphate guanylyltransferase n=1 Tax=Ga... [more]
UPI0012909F3C2.290e-7874.68mannose-1-phosphate guanylyltransferase/mannose-6-... [more]
B1JWE1_BURCC4.950e-7874.05Mannose-1-phosphate guanylyltransferase n=11 Tax=B... [more]
A0A0X3U6G1_9GAMM6.630e-7873.42Mannose-1-phosphate guanylyltransferase n=1 Tax=Mi... [more]
A0A6J5J733_9BURK1.010e-7773.42Mannose-1-phosphate guanylyltransferase n=89 Tax=B... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig123014contigM-pyrifera_M_contig123014:49..525 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score251.9
Seed ortholog evalue2e-64
Seed eggNOG ortholog216591.BCAL3246
Preferred namecpsB
KEGG rclassRC00002,RC00376
KEGG koko:K00971,ko:K16011
KEGG ReactionR00885,R01819
KEGG Pathwayko00051,ko00520,ko01100,ko01110,ko01130,ko02025,map00051,map00520,map01100,map01110,map01130,map02025
KEGG ModuleM00114,M00361,M00362
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0016740,GO:0016757
EggNOG free text desc.Belongs to the mannose-6-phosphate isomerase type 2 family
EggNOG OGs1K0NF@119060,1MV39@1224,2VI4S@28216,COG0836@1,COG0836@2
EC2.7.7.13,5.3.1.8
COG Functional cat.M
Best tax levelBurkholderiaceae
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000
Exons1
Model size477
Cds size477
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815749.3743744-CDS-M-pyrifera_M_contig123014:48..5251622815749.3743744-CDS-M-pyrifera_M_contig123014:48..525Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig123014 49..525 -
1692277551.4209504-CDS-M-pyrifera_M_contig123014:48..5251692277551.4209504-CDS-M-pyrifera_M_contig123014:48..525Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig123014 49..525 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig123014.4732.1prot_M-pyrifera_M_contig123014.4732.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig123014 49..525 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig123014.4732.1

>prot_M-pyrifera_M_contig123014.4732.1 ID=prot_M-pyrifera_M_contig123014.4732.1|Name=mRNA_M-pyrifera_M_contig123014.4732.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=159bp
AGLGCEDLVIVETPDAVMVAKKDRAQDVKKIVDWLNEQGREERLFHRRVY
RPWGSYEGVDAGTRFQVKRIVVNPGASLSLQMHRHRAEHWIVVSGTAKVT
CAEREFLLSENESTYIPIGATHRLENPGTIPCELIEVQSGSYLGEDDIVR
FEDKYNRA*
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mRNA from alignment at M-pyrifera_M_contig123014:49..525-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig123014.4732.1 ID=mRNA_M-pyrifera_M_contig123014.4732.1|Name=mRNA_M-pyrifera_M_contig123014.4732.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=477bp|location=Sequence derived from alignment at M-pyrifera_M_contig123014:49..525- (Macrocystis pyrifera P11B4 male)
GCCGGACTCGGCTGCGAAGACCTCGTGATCGTGGAAACCCCGGATGCGGT
GATGGTCGCGAAAAAAGACCGCGCTCAGGACGTGAAGAAAATTGTCGACT
GGCTGAACGAGCAGGGCCGCGAAGAACGCCTGTTCCATCGCCGCGTTTAC
CGCCCCTGGGGCAGTTACGAAGGCGTGGACGCCGGCACGAGGTTCCAGGT
GAAGCGGATCGTGGTGAATCCCGGCGCGTCTTTGTCGCTGCAAATGCACC
GCCACCGCGCGGAGCACTGGATCGTGGTGAGCGGCACCGCGAAGGTCACG
TGCGCGGAGCGGGAATTCCTGCTGTCGGAAAACGAATCCACCTACATCCC
GATCGGCGCGACCCACCGCCTGGAGAACCCCGGCACGATCCCCTGCGAAT
TAATTGAAGTGCAATCAGGGAGTTATCTTGGCGAGGACGATATTGTGCGG
TTTGAGGACAAGTACAATCGGGCGTAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig123014:49..525-

>mRNA_M-pyrifera_M_contig123014.4732.1 ID=mRNA_M-pyrifera_M_contig123014.4732.1|Name=mRNA_M-pyrifera_M_contig123014.4732.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=954bp|location=Sequence derived from alignment at M-pyrifera_M_contig123014:49..525- (Macrocystis pyrifera P11B4 male)
GCCGGACTCGGCTGCGAAGACCTCGTGATCGTGGAAACCCCGGATGCGGT
GATGGTCGCGAAAAAAGACCGCGCTCAGGACGTGAAGAAAATTGTCGACT
GGCTGAACGAGCAGGGCCGCGAAGAACGCCTGTTCCATCGCCGCGTTTAC
CGCCCCTGGGGCAGTTACGAAGGCGTGGACGCCGGCACGAGGTTCCAGGT
GAAGCGGATCGTGGTGAATCCCGGCGCGTCTTTGTCGCTGCAAATGCACC
GCCACCGCGCGGAGCACTGGATCGTGGTGAGCGGCACCGCGAAGGTCACG
TGCGCGGAGCGGGAATTCCTGCTGTCGGAAAACGAATCCACCTACATCCC
GATCGGCGCGACCCACCGCCTGGAGAACCCCGGCACGATCCCCTGCGAAT
TAATTGAAGTGCAATCAGGGAGTTATCTTGGCGAGGACGATATTGTGCGG
TTTGAGGACAAGTACAATCGGGCGTAAGCCGGACTCGGCTGCGAAGACCT
CGTGATCGTGGAAACCCCGGATGCGGTGATGGTCGCGAAAAAAGACCGCG
CTCAGGACGTGAAGAAAATTGTCGACTGGCTGAACGAGCAGGGCCGCGAA
GAACGCCTGTTCCATCGCCGCGTTTACCGCCCCTGGGGCAGTTACGAAGG
CGTGGACGCCGGCACGAGGTTCCAGGTGAAGCGGATCGTGGTGAATCCCG
GCGCGTCTTTGTCGCTGCAAATGCACCGCCACCGCGCGGAGCACTGGATC
GTGGTGAGCGGCACCGCGAAGGTCACGTGCGCGGAGCGGGAATTCCTGCT
GTCGGAAAACGAATCCACCTACATCCCGATCGGCGCGACCCACCGCCTGG
AGAACCCCGGCACGATCCCCTGCGAATTAATTGAAGTGCAATCAGGGAGT
TATCTTGGCGAGGACGATATTGTGCGGTTTGAGGACAAGTACAATCGGGC
GTAA
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