mRNA_M-pyrifera_M_contig122177.4570.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig122177.4570.1
Unique NamemRNA_M-pyrifera_M_contig122177.4570.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig122177.4570.1 vs. uniprot
Match: A0A7C2WKZ0_9BACT (Sodium-translocating pyrophosphatase n=1 Tax=Candidatus Hydrogenedentes bacterium TaxID=2030809 RepID=A0A7C2WKZ0_9BACT)

HSP 1 Score: 282 bits (722), Expect = 2.190e-93
Identity = 145/175 (82.86%), Postives = 159/175 (90.86%), Query Frame = 1 
Query:    1 AYHFAGVYGVSIAALGMLATTGMVVAVDSYGPIADNAGGIAEMAHLDPKVREITDNLDSVGNTTAAIGKGFAIGSAAFAAIGLLSAFMLAAGLTGADTALSEPNILAGVLIGAMMPFFFSSLLFRAVGSCADTMIQEVRRQFAEIPGLREGKEGVFPDSERCVDIATQGAIKGMM 525
            AYHFAGVYGV+IAALGMLATTGMVVAVD+YGPIADNAGGIAEMAHLDP VR+ITDNLD+VGNTTAAIGKGFAIGSAAFAAIGLLSAFML+AG+      +S+P ILAG+L+GAM+PFFFSS+LFRAVG CADTMI EVRRQF EIPGL+EGKEGV PDS  CV IATQGAI GM+
Sbjct:   25 AYHFAGVYGVAIAALGMLATTGMVVAVDAYGPIADNAGGIAEMAHLDPSVRKITDNLDAVGNTTAAIGKGFAIGSAAFAAIGLLSAFMLSAGIQAVS--ISDPQILAGILVGAMLPFFFSSMLFRAVGKCADTMIMEVRRQFREIPGLKEGKEGVMPDSTTCVSIATQGAINGML 197
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BLAST of mRNA_M-pyrifera_M_contig122177.4570.1 vs. uniprot
Match: A0A2A5D754_9BACT (Putative K(+)-stimulated pyrophosphate-energized sodium pump n=1 Tax=Candidatus Hydrogenedentes bacterium TaxID=2030809 RepID=A0A2A5D754_9BACT)

HSP 1 Score: 279 bits (714), Expect = 1.280e-87
Identity = 147/175 (84.00%), Postives = 159/175 (90.86%), Query Frame = 1 
Query:    1 AYHFAGVYGVSIAALGMLATTGMVVAVDSYGPIADNAGGIAEMAHLDPKVREITDNLDSVGNTTAAIGKGFAIGSAAFAAIGLLSAFMLAAGLTGADTALSEPNILAGVLIGAMMPFFFSSLLFRAVGSCADTMIQEVRRQFAEIPGLREGKEGVFPDSERCVDIATQGAIKGMM 525
            AY+  GVYG++IAALGMLATTGMVVAVDSYGPIADNAGGIAEMA LDPKVREITDNLDSVGNTTAAIGKGFAIGSAAFAAIGLLSAF+++A L  AD +L + +ILAGVLIG M+PF FSSLLFRAVG CA+ MI+EVRRQF EIPGLREGKEGV PDS RCVDIATQGAIKGMM
Sbjct:  403 AYNVCGVYGIAIAALGMLATTGMVVAVDSYGPIADNAGGIAEMAKLDPKVREITDNLDSVGNTTAAIGKGFAIGSAAFAAIGLLSAFLVSAQLEPADVSLMDSHILAGVLIGGMLPFLFSSLLFRAVGDCANMMIEEVRRQFREIPGLREGKEGVEPDSSRCVDIATQGAIKGMM 577
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BLAST of mRNA_M-pyrifera_M_contig122177.4570.1 vs. uniprot
Match: A0A1V5YT59_9BACT (Putative K(+)-stimulated pyrophosphate-energized sodium pump n=1 Tax=Candidatus Hydrogenedentes bacterium ADurb.Bin170 TaxID=1852848 RepID=A0A1V5YT59_9BACT)

HSP 1 Score: 278 bits (711), Expect = 3.260e-87
Identity = 146/175 (83.43%), Postives = 161/175 (92.00%), Query Frame = 1 
Query:    1 AYHFAGVYGVSIAALGMLATTGMVVAVDSYGPIADNAGGIAEMAHLDPKVREITDNLDSVGNTTAAIGKGFAIGSAAFAAIGLLSAFMLAAGLTGADTALSEPNILAGVLIGAMMPFFFSSLLFRAVGSCADTMIQEVRRQFAEIPGLREGKEGVFPDSERCVDIATQGAIKGMM 525
            A H+AGVYGV+IAALGMLATTGMVVAVD+YGPIADNAGGIAEMAH+DPKVR ITDNLD+VGNTTAAIGKGFAIGSAAFAAIGLLSAFML+A +T  + ++++P+ILAG+LIGAMMPFFFSSLLF AVG CADTMIQEVRRQF EIPGLREG EGV PDS  CV IATQGAIKGM+
Sbjct:  403 AAHYAGVYGVAIAALGMLATTGMVVAVDAYGPIADNAGGIAEMAHMDPKVRHITDNLDAVGNTTAAIGKGFAIGSAAFAAIGLLSAFMLSAKVT--EVSIADPHILAGLLIGAMMPFFFSSLLFGAVGRCADTMIQEVRRQFREIPGLREGLEGVHPDSTTCVSIATQGAIKGML 575
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BLAST of mRNA_M-pyrifera_M_contig122177.4570.1 vs. uniprot
Match: A0A831UJD1_9BACT (Putative K(+)-stimulated pyrophosphate-energized sodium pump n=1 Tax=Candidatus Hydrogenedentes bacterium TaxID=2030809 RepID=A0A831UJD1_9BACT)

HSP 1 Score: 272 bits (695), Expect = 7.350e-85
Identity = 142/175 (81.14%), Postives = 156/175 (89.14%), Query Frame = 1 
Query:    1 AYHFAGVYGVSIAALGMLATTGMVVAVDSYGPIADNAGGIAEMAHLDPKVREITDNLDSVGNTTAAIGKGFAIGSAAFAAIGLLSAFMLAAGLTGADTALSEPNILAGVLIGAMMPFFFSSLLFRAVGSCADTMIQEVRRQFAEIPGLREGKEGVFPDSERCVDIATQGAIKGMM 525
            AY+ AG+YG++IAALGMLATTGMVVAVDSYGPIADNAGGIAEMA LDP+VREITD LD+VGNTTAAIGKGFAIGSAAFAAIGLLSAFM++AGL  AD +L EP ILAGVL+G M+PF FSS+LFRAVG CA  MI EVRRQF EIPGL+EG+EGV PDS  CVDIATQGAI GMM
Sbjct:  402 AYNCAGMYGIAIAALGMLATTGMVVAVDSYGPIADNAGGIAEMAGLDPRVREITDGLDAVGNTTAAIGKGFAIGSAAFAAIGLLSAFMVSAGLDAADVSLLEPKILAGVLVGGMLPFLFSSMLFRAVGRCAYKMIDEVRRQFREIPGLKEGEEGVVPDSTTCVDIATQGAIHGMM 576
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BLAST of mRNA_M-pyrifera_M_contig122177.4570.1 vs. uniprot
Match: A0A7V2X701_9BACT (Sodium-translocating pyrophosphatase n=1 Tax=Candidatus Hydrogenedentes bacterium TaxID=2030809 RepID=A0A7V2X701_9BACT)

HSP 1 Score: 269 bits (687), Expect = 1.170e-84
Identity = 140/175 (80.00%), Postives = 158/175 (90.29%), Query Frame = 1 
Query:    1 AYHFAGVYGVSIAALGMLATTGMVVAVDSYGPIADNAGGIAEMAHLDPKVREITDNLDSVGNTTAAIGKGFAIGSAAFAAIGLLSAFMLAAGLTGADTALSEPNILAGVLIGAMMPFFFSSLLFRAVGSCADTMIQEVRRQFAEIPGLREGKEGVFPDSERCVDIATQGAIKGMM 525
            AY+ AG+YG++IAALGMLATTGMVVAVD+YGPIADNAGGIAEM+HLDP VR+ITDNLD+VGNTTAAIGKGFAIGSAA+AAIGLLSAF+LAA       ++S+P ILAG+LIGAMMPF+FSS LF AVG+CADTMIQEVRRQF E PGLREGKEGV PDS RCV IATQGAI+GM+
Sbjct:  292 AYYCAGIYGIAIAALGMLATTGMVVAVDAYGPIADNAGGIAEMSHLDPSVRKITDNLDAVGNTTAAIGKGFAIGSAAYAAIGLLSAFILAA--KPEHVSISDPKILAGLLIGAMMPFYFSSKLFNAVGTCADTMIQEVRRQFRETPGLREGKEGVVPDSARCVAIATQGAIRGML 464
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BLAST of mRNA_M-pyrifera_M_contig122177.4570.1 vs. uniprot
Match: A0A7Y5C4J8_9BACT (Putative K(+)-stimulated pyrophosphate-energized sodium pump n=1 Tax=Candidatus Hydrogenedentes bacterium TaxID=2030809 RepID=A0A7Y5C4J8_9BACT)

HSP 1 Score: 270 bits (691), Expect = 2.760e-84
Identity = 140/175 (80.00%), Postives = 158/175 (90.29%), Query Frame = 1 
Query:    1 AYHFAGVYGVSIAALGMLATTGMVVAVDSYGPIADNAGGIAEMAHLDPKVREITDNLDSVGNTTAAIGKGFAIGSAAFAAIGLLSAFMLAAGLTGADTALSEPNILAGVLIGAMMPFFFSSLLFRAVGSCADTMIQEVRRQFAEIPGLREGKEGVFPDSERCVDIATQGAIKGMM 525
            AYH AGVYGV+IAALGMLATTG+VVAVDSYGPIADNAGGIAEM+HLDP VR+ITDNLD+VGNTTAAIGKGFAIGSAAFAAIGLLSAF+++A LT    A+S P IL+G+LIG M+PFFFSS+LFRAV   A  MI EVRRQFAEIPGL+EGKEGV+PDS +CVDIAT+GAI+GMM
Sbjct:  398 AYHVAGVYGVAIAALGMLATTGIVVAVDSYGPIADNAGGIAEMSHLDPSVRKITDNLDAVGNTTAAIGKGFAIGSAAFAAIGLLSAFLISARLTEHSIAISNPYILSGILIGGMVPFFFSSMLFRAVSKSAFAMIGEVRRQFAEIPGLKEGKEGVYPDSAKCVDIATRGAIQGMM 572
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BLAST of mRNA_M-pyrifera_M_contig122177.4570.1 vs. uniprot
Match: A0A7V7EAH8_9BACT (Putative K(+)-stimulated pyrophosphate-energized sodium pump n=1 Tax=Candidatus Hydrogenedentes bacterium TaxID=2030809 RepID=A0A7V7EAH8_9BACT)

HSP 1 Score: 270 bits (690), Expect = 5.100e-84
Identity = 144/175 (82.29%), Postives = 153/175 (87.43%), Query Frame = 1 
Query:    1 AYHFAGVYGVSIAALGMLATTGMVVAVDSYGPIADNAGGIAEMAHLDPKVREITDNLDSVGNTTAAIGKGFAIGSAAFAAIGLLSAFMLAAGLTGADTALSEPNILAGVLIGAMMPFFFSSLLFRAVGSCADTMIQEVRRQFAEIPGLREGKEGVFPDSERCVDIATQGAIKGMM 525
            AY FAGVYGV+IAALGMLATTGMVVAVDSYGPIADNAGGIAEMA LDPKVR+ITDNLD+VGNTTAAIGKGFAIGSAAFAAIGLLSAFMLAA L  A  +L+ P ILAGVLIG MMPF FSS+LFRAV  CA  MI EVRRQF EIPGL+EG+EG  PDS  CVDIAT+GAI GMM
Sbjct:  399 AYQFAGVYGVAIAALGMLATTGMVVAVDSYGPIADNAGGIAEMAGLDPKVRKITDNLDAVGNTTAAIGKGFAIGSAAFAAIGLLSAFMLAARLDEAQVSLASPTILAGVLIGGMMPFLFSSMLFRAVRKCAYGMIDEVRRQFREIPGLKEGREGALPDSATCVDIATKGAINGMM 573
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BLAST of mRNA_M-pyrifera_M_contig122177.4570.1 vs. uniprot
Match: A0A3M1A1V7_9BACT (Sodium-translocating pyrophosphatase (Fragment) n=1 Tax=Calditrichaeota bacterium TaxID=2212469 RepID=A0A3M1A1V7_9BACT)

HSP 1 Score: 246 bits (629), Expect = 3.190e-75
Identity = 129/174 (74.14%), Postives = 150/174 (86.21%), Query Frame = 1 
Query:    4 YHFAGVYGVSIAALGMLATTGMVVAVDSYGPIADNAGGIAEMAHLDPKVREITDNLDSVGNTTAAIGKGFAIGSAAFAAIGLLSAFMLAAGLTGADTALSEPNILAGVLIGAMMPFFFSSLLFRAVGSCADTMIQEVRRQFAEIPGLREGKEGVFPDSERCVDIATQGAIKGMM 525
            YHFAG+YGV++AALGMLATTGMVV VDSYGP+ADNAGGIAEMA LDPKVR ITDNLD+VGNTTAAIGKGFAIGSAAFAA+GLL A+M +A +  AD  + +P +LAG+LIG M+PFFFSS+LF+AV   A  MI+EVRRQF EIPGL EGK  V PDS RCVDI+T+GAI+GM+
Sbjct:  399 YHFAGIYGVAMAALGMLATTGMVVTVDSYGPVADNAGGIAEMAELDPKVRNITDNLDAVGNTTAAIGKGFAIGSAAFAAMGLLVAYMRSANVQVAD--IKDPKVLAGLLIGGMLPFFFSSMLFKAVSKAAFKMIEEVRRQFREIPGLMEGK--VMPDSARCVDISTKGAIQGML 568
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BLAST of mRNA_M-pyrifera_M_contig122177.4570.1 vs. uniprot
Match: A0A7C3D3G5_9BACT (Sodium-translocating pyrophosphatase n=1 Tax=Bacteroidetes bacterium TaxID=1898104 RepID=A0A7C3D3G5_9BACT)

HSP 1 Score: 239 bits (610), Expect = 5.480e-75
Identity = 127/175 (72.57%), Postives = 145/175 (82.86%), Query Frame = 1 
Query:    1 AYHFAGVYGVSIAALGMLATTGMVVAVDSYGPIADNAGGIAEMAHLDPKVREITDNLDSVGNTTAAIGKGFAIGSAAFAAIGLLSAFMLAAGLTGADTALSEPNILAGVLIGAMMPFFFSSLLFRAVGSCADTMIQEVRRQFAEIPGLREGKEGVFPDSERCVDIATQGAIKGMM 525
            AYHFAGVYGV +AA GMLATTGMVV+VDSYGP+ADNAGGIAEMA LDP VR+ITDNLD+VGNTTAAIGKGFAIGSAAFAA+GLL A+M A GL  AD  + +PN+L G+L+G M+PF FSS+LF+AV   A  MI EVRRQF EIPGL EGK    PD E CVDI+T+GAI GM+
Sbjct:  138 AYHFAGVYGVGMAAFGMLATTGMVVSVDSYGPVADNAGGIAEMAELDPSVRKITDNLDAVGNTTAAIGKGFAIGSAAFAAMGLLVAYMHAVGLKVAD--IKDPNVLMGLLLGGMLPFLFSSMLFQAVSKAAFKMIDEVRRQFREIPGLLEGK--AMPDPESCVDISTRGAIHGML 308
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BLAST of mRNA_M-pyrifera_M_contig122177.4570.1 vs. uniprot
Match: A0A1V5FKW1_9BACT (Putative K(+)-stimulated pyrophosphate-energized sodium pump n=1 Tax=candidate division BRC1 bacterium ADurb.BinA292 TaxID=1852824 RepID=A0A1V5FKW1_9BACT)

HSP 1 Score: 241 bits (615), Expect = 2.350e-73
Identity = 125/175 (71.43%), Postives = 147/175 (84.00%), Query Frame = 1 
Query:    1 AYHFAGVYGVSIAALGMLATTGMVVAVDSYGPIADNAGGIAEMAHLDPKVREITDNLDSVGNTTAAIGKGFAIGSAAFAAIGLLSAFMLAAGLTGADTALSEPNILAGVLIGAMMPFFFSSLLFRAVGSCADTMIQEVRRQFAEIPGLREGKEGVFPDSERCVDIATQGAIKGMM 525
            AYHFAG YG+++AA GMLATTG++V+VDSYGPIADNAGGIAEMAHLD  VREITDNLDSVGNTTAAIGKGFAIGSAAFAA+GLL A+  +AG++ AD +L+EP +LAG+L+G M P+FFSS+LFRAV   A  MI EVRRQ  EIPG+ EGK    PD  +CVDIAT+GAI GM+
Sbjct:  367 AYHFAGFYGIALAAFGMLATTGIIVSVDSYGPIADNAGGIAEMAHLDRSVREITDNLDSVGNTTAAIGKGFAIGSAAFAALGLLVAYTASAGISAADVSLTEPAVLAGLLLGGMFPYFFSSMLFRAVSISAFAMIGEVRRQLREIPGIMEGK--AKPDYAQCVDIATRGAINGML 539
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig122177.4570.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7C2WKZ0_9BACT2.190e-9382.86Sodium-translocating pyrophosphatase n=1 Tax=Candi... [more]
A0A2A5D754_9BACT1.280e-8784.00Putative K(+)-stimulated pyrophosphate-energized s... [more]
A0A1V5YT59_9BACT3.260e-8783.43Putative K(+)-stimulated pyrophosphate-energized s... [more]
A0A831UJD1_9BACT7.350e-8581.14Putative K(+)-stimulated pyrophosphate-energized s... [more]
A0A7V2X701_9BACT1.170e-8480.00Sodium-translocating pyrophosphatase n=1 Tax=Candi... [more]
A0A7Y5C4J8_9BACT2.760e-8480.00Putative K(+)-stimulated pyrophosphate-energized s... [more]
A0A7V7EAH8_9BACT5.100e-8482.29Putative K(+)-stimulated pyrophosphate-energized s... [more]
A0A3M1A1V7_9BACT3.190e-7574.14Sodium-translocating pyrophosphatase (Fragment) n=... [more]
A0A7C3D3G5_9BACT5.480e-7572.57Sodium-translocating pyrophosphatase n=1 Tax=Bacte... [more]
A0A1V5FKW1_9BACT2.350e-7371.43Putative K(+)-stimulated pyrophosphate-energized s... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig122177contigM-pyrifera_M_contig122177:5..529 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score211.8
Seed ortholog evalue2.5e-52
Seed eggNOG ortholog373903.Hore_15760
Preferred namehppA
KEGG koko:K15987
KEGG TC3.A.10.1
KEGG Pathwayko00190,map00190
Hectar predicted targeting categorysignal peptide
EggNOG free text desc.Sodium pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for Na( ) movement across the membrane
EggNOG OGs1TNZI@1239,248KS@186801,3WA8T@53433,COG3808@1,COG3808@2
EC3.6.1.1
COG Functional cat.C
Best tax levelHalanaerobiales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Exons1
Model size525
Cds size525
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815741.688727-CDS-M-pyrifera_M_contig122177:4..5291622815741.688727-CDS-M-pyrifera_M_contig122177:4..529Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig122177 5..529 +
1692277547.8281288-CDS-M-pyrifera_M_contig122177:4..5291692277547.8281288-CDS-M-pyrifera_M_contig122177:4..529Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig122177 5..529 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig122177.4570.1prot_M-pyrifera_M_contig122177.4570.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig122177 5..529 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig122177.4570.1

>prot_M-pyrifera_M_contig122177.4570.1 ID=prot_M-pyrifera_M_contig122177.4570.1|Name=mRNA_M-pyrifera_M_contig122177.4570.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=175bp
AYHFAGVYGVSIAALGMLATTGMVVAVDSYGPIADNAGGIAEMAHLDPKV
REITDNLDSVGNTTAAIGKGFAIGSAAFAAIGLLSAFMLAAGLTGADTAL
SEPNILAGVLIGAMMPFFFSSLLFRAVGSCADTMIQEVRRQFAEIPGLRE
GKEGVFPDSERCVDIATQGAIKGMM
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mRNA from alignment at M-pyrifera_M_contig122177:5..529+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig122177.4570.1 ID=mRNA_M-pyrifera_M_contig122177.4570.1|Name=mRNA_M-pyrifera_M_contig122177.4570.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=525bp|location=Sequence derived from alignment at M-pyrifera_M_contig122177:5..529+ (Macrocystis pyrifera P11B4 male)
GCGTATCACTTCGCCGGCGTCTACGGCGTCTCAATCGCCGCTCTCGGAAT
GTTGGCCACGACCGGAATGGTCGTCGCGGTTGACAGTTACGGTCCGATCG
CGGACAACGCGGGTGGTATCGCTGAAATGGCCCACCTCGATCCGAAGGTT
CGTGAAATAACCGATAACCTCGACTCGGTCGGTAACACGACTGCGGCGAT
TGGTAAAGGCTTCGCCATCGGTAGTGCCGCATTCGCGGCCATCGGATTGT
TGAGCGCGTTCATGCTTGCAGCCGGACTGACCGGTGCCGATACCGCATTG
AGCGAACCCAACATTCTCGCCGGTGTGCTCATTGGTGCGATGATGCCGTT
CTTTTTCTCGTCATTGTTATTCCGCGCCGTGGGTTCATGCGCGGATACGA
TGATTCAAGAAGTGCGTCGCCAATTCGCGGAGATTCCCGGATTGCGCGAA
GGTAAAGAAGGCGTGTTCCCGGATAGCGAACGGTGCGTCGACATCGCAAC
CCAAGGCGCGATCAAGGGCATGATG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig122177:5..529+

>mRNA_M-pyrifera_M_contig122177.4570.1 ID=mRNA_M-pyrifera_M_contig122177.4570.1|Name=mRNA_M-pyrifera_M_contig122177.4570.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1050bp|location=Sequence derived from alignment at M-pyrifera_M_contig122177:5..529+ (Macrocystis pyrifera P11B4 male)
GCGTATCACTTCGCCGGCGTCTACGGCGTCTCAATCGCCGCTCTCGGAAT
GTTGGCCACGACCGGAATGGTCGTCGCGGTTGACAGTTACGGTCCGATCG
CGGACAACGCGGGTGGTATCGCTGAAATGGCCCACCTCGATCCGAAGGTT
CGTGAAATAACCGATAACCTCGACTCGGTCGGTAACACGACTGCGGCGAT
TGGTAAAGGCTTCGCCATCGGTAGTGCCGCATTCGCGGCCATCGGATTGT
TGAGCGCGTTCATGCTTGCAGCCGGACTGACCGGTGCCGATACCGCATTG
AGCGAACCCAACATTCTCGCCGGTGTGCTCATTGGTGCGATGATGCCGTT
CTTTTTCTCGTCATTGTTATTCCGCGCCGTGGGTTCATGCGCGGATACGA
TGATTCAAGAAGTGCGTCGCCAATTCGCGGAGATTCCCGGATTGCGCGAA
GGTAAAGAAGGCGTGTTCCCGGATAGCGAACGGTGCGTCGACATCGCAAC
CCAAGGCGCGATCAAGGGCATGATGGCGTATCACTTCGCCGGCGTCTACG
GCGTCTCAATCGCCGCTCTCGGAATGTTGGCCACGACCGGAATGGTCGTC
GCGGTTGACAGTTACGGTCCGATCGCGGACAACGCGGGTGGTATCGCTGA
AATGGCCCACCTCGATCCGAAGGTTCGTGAAATAACCGATAACCTCGACT
CGGTCGGTAACACGACTGCGGCGATTGGTAAAGGCTTCGCCATCGGTAGT
GCCGCATTCGCGGCCATCGGATTGTTGAGCGCGTTCATGCTTGCAGCCGG
ACTGACCGGTGCCGATACCGCATTGAGCGAACCCAACATTCTCGCCGGTG
TGCTCATTGGTGCGATGATGCCGTTCTTTTTCTCGTCATTGTTATTCCGC
GCCGTGGGTTCATGCGCGGATACGATGATTCAAGAAGTGCGTCGCCAATT
CGCGGAGATTCCCGGATTGCGCGAAGGTAAAGAAGGCGTGTTCCCGGATA
GCGAACGGTGCGTCGACATCGCAACCCAAGGCGCGATCAAGGGCATGATG
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