mRNA_M-pyrifera_M_contig120403.4209.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig120403.4209.1
Unique NamemRNA_M-pyrifera_M_contig120403.4209.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig120403.4209.1 vs. uniprot
Match: UPI0009E4E6A1 (V-type proton ATPase subunit d 1-like n=1 Tax=Orbicella faveolata TaxID=48498 RepID=UPI0009E4E6A1)

HSP 1 Score: 140 bits (353), Expect = 1.690e-39
Identity = 69/125 (55.20%), Postives = 89/125 (71.20%), Query Frame = 1 
Query:    1 APYFRDCLSRDALQDLAIEIIRNLLHKAYLEDFYRYCREELGGTTAETMSELLEFEADRRAITITQNCMGKDIGKDDREKLYCNFGRLYPEATEKLAKASDMDEVRAVLELPGYGDYKLVLEATG 375
            AP+F +C+S   L ++ IEIIRN L+KAYLE FY++C E+LGGTTA+ M  +L+FEADRRA  IT N  G ++GKDDR KLY   G+LYPE   +LAKA D ++VRAV +   Y DYK + E  G
Sbjct:   21 APFFVECISEQDLDEMNIEIIRNTLYKAYLEGFYKFC-EDLGGTTADVMCPILQFEADRRAFIITINSFGTELGKDDRNKLYPKCGKLYPEGLSRLAKADDYEQVRAVADY--YSDYKSLFEGAG 142
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BLAST of mRNA_M-pyrifera_M_contig120403.4209.1 vs. uniprot
Match: A0A059ARZ2_EUCGR (Uncharacterized protein n=1 Tax=Eucalyptus grandis TaxID=71139 RepID=A0A059ARZ2_EUCGR)

HSP 1 Score: 139 bits (351), Expect = 6.050e-39
Identity = 68/127 (53.54%), Postives = 93/127 (73.23%), Query Frame = 1 
Query:    1 APYFRDCLSRDALQDLAIEIIRNLLHKAYLEDFYRYCREELGGTTAETMSELLEFEADRRAITITQNCMGKDIGKDDREKLYCNFGRLYPEATEKLAKASDMDEVRAVLELPGYGDYKLVLEATGFG 381
            APYF +C++ + L D+ IEI+RN L+KAYLEDFYR+C+ +LGG TAE MS+LL FEADRRA+ IT N +G ++ +DDR+KLY NFG LYP   E+LA + D+D+VR V+E   Y  Y+ +     +G
Sbjct:   77 APYFSECITSEDLDDMNIEIMRNTLYKAYLEDFYRFCQ-KLGGATAEIMSDLLGFEADRRAVNITINSIGTELTRDDRKKLYSNFGLLYPYGHEELAVSEDIDQVRGVMEK--YPPYQSIFSKLSYG 200
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BLAST of mRNA_M-pyrifera_M_contig120403.4209.1 vs. uniprot
Match: D0UM71_9ARAC (ATP synthase (Fragment) n=3 Tax=Arachnida TaxID=6854 RepID=D0UM71_9ARAC)

HSP 1 Score: 137 bits (344), Expect = 1.130e-38
Identity = 66/125 (52.80%), Postives = 90/125 (72.00%), Query Frame = 1 
Query:    1 APYFRDCLSRDALQDLAIEIIRNLLHKAYLEDFYRYCREELGGTTAETMSELLEFEADRRAITITQNCMGKDIGKDDREKLYCNFGRLYPEATEKLAKASDMDEVRAVLELPGYGDYKLVLEATG 375
            AP+F DC+S   + D+ IEIIRN L+KAYLE FY++C+ ELGG+TA+ M E+L FEADRRA  IT N  G ++ K+DR KLY + G+LYP+  + LAKA D ++V++V E   YG+YK + E  G
Sbjct:   21 APFFGDCISEQDIDDMNIEIIRNTLYKAYLEAFYKFCK-ELGGSTADVMCEILAFEADRRAFVITINSFGTELTKEDRAKLYPHCGKLYPDGLQALAKADDYEQVKSVAEY--YGEYKALFEGAG 142
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BLAST of mRNA_M-pyrifera_M_contig120403.4209.1 vs. uniprot
Match: A0A1S3HXH1_LINUN (V-type proton ATPase subunit n=1 Tax=Lingula unguis TaxID=7574 RepID=A0A1S3HXH1_LINUN)

HSP 1 Score: 141 bits (355), Expect = 2.490e-38
Identity = 70/127 (55.12%), Postives = 91/127 (71.65%), Query Frame = 1 
Query:    1 APYFRDCLSRDALQDLAIEIIRNLLHKAYLEDFYRYCREELGGTTAETMSELLEFEADRRAITITQNCMGKDIGKDDREKLYCNFGRLYPEATEKLAKASDMDEVRAVLELPGYGDYKLVLEATGFG 381
            APYF DC+S   L ++ IEIIRN L+KAYLEDF+++C+ E+GGTTAE M E L FEADRRA  IT N  G ++ K+DR KLY   G+LYP+    L++A D ++VRAV E   YG+YKL+ E +G G
Sbjct:  158 APYFIDCISEQDLDEMNIEIIRNTLYKAYLEDFHKFCK-EMGGTTAEVMCEALAFEADRRAFIITINSFGTELSKEDRAKLYPRCGKLYPDGLAALSRADDYEQVRAVSEY--YGEYKLLFEGSGTG 281
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BLAST of mRNA_M-pyrifera_M_contig120403.4209.1 vs. uniprot
Match: A0A090LFN5_STRRB (V-type proton ATPase subunit n=6 Tax=Strongyloidoidea TaxID=2082224 RepID=A0A090LFN5_STRRB)

HSP 1 Score: 141 bits (355), Expect = 2.540e-38
Identity = 69/125 (55.20%), Postives = 88/125 (70.40%), Query Frame = 1 
Query:    1 APYFRDCLSRDALQDLAIEIIRNLLHKAYLEDFYRYCREELGGTTAETMSELLEFEADRRAITITQNCMGKDIGKDDREKLYCNFGRLYPEATEKLAKASDMDEVRAVLELPGYGDYKLVLEATG 375
            APYF DC++   L ++ +EIIRN L+KAY+EDFY++C+  LGGTTAE M  +L FEADRRAITIT N    ++ KDDREKLY   G+LYPE    LA+A D D+V+ + E   Y DYK + E TG
Sbjct:  158 APYFVDCINEQDLDEMNVEIIRNTLYKAYIEDFYKFCK-TLGGTTAEVMCGILAFEADRRAITITINSFDTELSKDDREKLYPRVGKLYPEGLASLARADDYDQVKQICEF--YSDYKGLFEGTG 279
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BLAST of mRNA_M-pyrifera_M_contig120403.4209.1 vs. uniprot
Match: A0A2C9U431_MANES (V-type proton ATPase subunit n=18 Tax=Mesangiospermae TaxID=1437183 RepID=A0A2C9U431_MANES)

HSP 1 Score: 141 bits (355), Expect = 2.650e-38
Identity = 69/127 (54.33%), Postives = 92/127 (72.44%), Query Frame = 1 
Query:    1 APYFRDCLSRDALQDLAIEIIRNLLHKAYLEDFYRYCREELGGTTAETMSELLEFEADRRAITITQNCMGKDIGKDDREKLYCNFGRLYPEATEKLAKASDMDEVRAVLELPGYGDYKLVLEATGFG 381
            APYF +C++ + L D+ IEI+RN L+KAYLEDFYR+C+ +LGG TAE MS+LL FEADRRA+ IT N +G ++ +DDR KLY NFG LYP   E+LA   D+D+VRAV+E   Y  Y+ +     +G
Sbjct:  161 APYFSECITSEDLDDMNIEIMRNTLYKAYLEDFYRFCQ-KLGGATAEIMSDLLAFEADRRAVNITINSIGTELTRDDRRKLYSNFGLLYPYGHEELAVCEDLDQVRAVMEK--YPPYQSIFSKLSYG 284
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BLAST of mRNA_M-pyrifera_M_contig120403.4209.1 vs. uniprot
Match: A0A1R1Y9Y2_9FUNG (V-type proton ATPase subunit d 1 n=2 Tax=Smittium culicis TaxID=133412 RepID=A0A1R1Y9Y2_9FUNG)

HSP 1 Score: 140 bits (352), Expect = 3.020e-38
Identity = 67/125 (53.60%), Postives = 90/125 (72.00%), Query Frame = 1 
Query:    1 APYFRDCLSRDALQDLAIEIIRNLLHKAYLEDFYRYCREELGGTTAETMSELLEFEADRRAITITQNCMGKDIGKDDREKLYCNFGRLYPEATEKLAKASDMDEVRAVLELPGYGDYKLVLEATG 375
            APYFRDCLS + L ++ IEIIRN L++AYLEDFY +C  +L   TAE MS LL FEAD R+I+IT N  G ++ KD+R  LY   G LYP+A +KL+K  ++D+V+A+L+   Y DY+L+L  TG
Sbjct:  115 APYFRDCLSAEDLNEINIEIIRNTLYRAYLEDFYEFCNTKLNSKTAEVMSNLLNFEADSRSISITINSFGTELTKDERRMLYPKLGFLYPDAQDKLSKVDELDQVKAILDT--YPDYRLLLSNTG 237
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BLAST of mRNA_M-pyrifera_M_contig120403.4209.1 vs. uniprot
Match: A0A7R9LFR9_9ACAR (V-type proton ATPase subunit n=1 Tax=Oppiella nova TaxID=334625 RepID=A0A7R9LFR9_9ACAR)

HSP 1 Score: 140 bits (352), Expect = 8.020e-38
Identity = 65/128 (50.78%), Postives = 90/128 (70.31%), Query Frame = 1 
Query:    1 APYFRDCLSRDALQDLAIEIIRNLLHKAYLEDFYRYCREELGGTTAETMSELLEFEADRRAITITQNCMGKDIGKDDREKLYCNFGRLYPEATEKLAKASDMDEVRAVLELPGYGDYKLVLEATGFGG 384
            AP+F DC+    L ++ +EIIRN L+KAYLE FY++C++++GG TA+ M E+L FEADRRA  IT N  G ++ K+DR KLY N GRLYP+    LA+A D ++VR+V E   YG+YK++ E  G  G
Sbjct:  159 APFFVDCIGEQDLDEMNVEIIRNTLYKAYLESFYKFCKDQIGGITADVMCEILAFEADRRAFIITINSFGTELSKEDRAKLYPNCGRLYPDGLAALARADDYEQVRSVAEY--YGEYKVLFEGQGQSG 284
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BLAST of mRNA_M-pyrifera_M_contig120403.4209.1 vs. uniprot
Match: A0A4P9XYI8_9FUNG (V-type proton ATPase subunit n=1 Tax=Piptocephalis cylindrospora TaxID=1907219 RepID=A0A4P9XYI8_9FUNG)

HSP 1 Score: 139 bits (351), Expect = 9.970e-38
Identity = 69/128 (53.91%), Postives = 90/128 (70.31%), Query Frame = 1 
Query:    1 APYFRDCLSRDALQDLAIEIIRNLLHKAYLEDFYRYCREELGGTTAETMSELLEFEADRRAITITQNCMGKDIGKDDREKLYCNFGRLYPEATEKLAKASDMDEVRAVLELPGYGDYKLVLEATGFGG 384
            APYF DCLS   L ++ IEIIRN L+KAYLEDF RYCREE+GG T E M+E+LEFEADRRAI IT N  G ++ KD+R +L+   G+LYPE   +LA+A DMD V+A  E   + +Y+   +++   G
Sbjct:  158 APYFSDCLSAHDLDEMNIEIIRNTLYKAYLEDFARYCREEVGGVTGEIMTEILEFEADRRAINITVNSFGTELTKDERSRLFPCLGKLYPEGHVRLARADDMDGVKAASE--SFVEYRAFFDSSASAG 283
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BLAST of mRNA_M-pyrifera_M_contig120403.4209.1 vs. uniprot
Match: A2Q5T7_MEDTR (V-type proton ATPase subunit n=143 Tax=Spermatophyta TaxID=58024 RepID=A2Q5T7_MEDTR)

HSP 1 Score: 139 bits (349), Expect = 2.060e-37
Identity = 68/127 (53.54%), Postives = 91/127 (71.65%), Query Frame = 1 
Query:    1 APYFRDCLSRDALQDLAIEIIRNLLHKAYLEDFYRYCREELGGTTAETMSELLEFEADRRAITITQNCMGKDIGKDDREKLYCNFGRLYPEATEKLAKASDMDEVRAVLELPGYGDYKLVLEATGFG 381
            APYF +C++ + L D+ IEI+RN L+KAYLEDFYR+C+ +LGG TAE MS+LL FEADRRA+ IT N +G ++ +DDR KLY NFG  YP   E+LA   D+D+VRAV+E   Y  Y+ +     +G
Sbjct:  161 APYFSECITSEDLDDMNIEIMRNTLYKAYLEDFYRFCQ-KLGGATAEIMSDLLAFEADRRAVNITINSIGTELTRDDRRKLYSNFGLFYPYGHEELAVCEDIDQVRAVMEK--YPPYQSIFAKLSYG 284
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig120403.4209.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UPI0009E4E6A11.690e-3955.20V-type proton ATPase subunit d 1-like n=1 Tax=Orbi... [more]
A0A059ARZ2_EUCGR6.050e-3953.54Uncharacterized protein n=1 Tax=Eucalyptus grandis... [more]
D0UM71_9ARAC1.130e-3852.80ATP synthase (Fragment) n=3 Tax=Arachnida TaxID=68... [more]
A0A1S3HXH1_LINUN2.490e-3855.12V-type proton ATPase subunit n=1 Tax=Lingula ungui... [more]
A0A090LFN5_STRRB2.540e-3855.20V-type proton ATPase subunit n=6 Tax=Strongyloidoi... [more]
A0A2C9U431_MANES2.650e-3854.33V-type proton ATPase subunit n=18 Tax=Mesangiosper... [more]
A0A1R1Y9Y2_9FUNG3.020e-3853.60V-type proton ATPase subunit d 1 n=2 Tax=Smittium ... [more]
A0A7R9LFR9_9ACAR8.020e-3850.78V-type proton ATPase subunit n=1 Tax=Oppiella nova... [more]
A0A4P9XYI8_9FUNG9.970e-3853.91V-type proton ATPase subunit n=1 Tax=Piptocephalis... [more]
A2Q5T7_MEDTR2.060e-3753.54V-type proton ATPase subunit n=143 Tax=Spermatophy... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig120403contigM-pyrifera_M_contig120403:34..535 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeViridiplantae
Seed ortholog score142.1
Seed ortholog evalue1.8e-31
Seed eggNOG ortholog3983.cassava4.1_010810m
KEGG koko:K02146
KEGG TC3.A.2.2
KEGG Pathwayko00190,ko01100,ko04142,ko04145,ko04721,ko04966,ko05110,ko05120,ko05152,ko05203,ko05323,map00190,map01100,map04142,map04145,map04721,map04966,map05110,map05120,map05152,map05203,map05323
KEGG ModuleM00160
Hectar predicted targeting categoryother localisation
GOsGO:0000323,GO:0000325,GO:0003674,GO:0003824,GO:0005215,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005764,GO:0005765,GO:0005773,GO:0005774,GO:0005794,GO:0005886,GO:0005911,GO:0006810,GO:0006811,GO:0006812,GO:0006873,GO:0006885,GO:0007034,GO:0007035,GO:0008150,GO:0008324,GO:0008553,GO:0009506,GO:0009987,GO:0012505,GO:0015075,GO:0015077,GO:0015078,GO:0015318,GO:0015399,GO:0015405,GO:0015662,GO:0015672,GO:0016020,GO:0016462,GO:0016469,GO:0016471,GO:0016787,GO:0016817,GO:0016818,GO:0016887,GO:0017111,GO:0019725,GO:0019829,GO:0022804,GO:0022853,GO:0022857,GO:0022890,GO:0030003,GO:0030004,GO:0030054,GO:0030641,GO:0031090,GO:0032991,GO:0033176,GO:0034220,GO:0036442,GO:0042592,GO:0042623,GO:0042625,GO:0042626,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043492,GO:0044422,GO:0044424,GO:0044425,GO:0044437,GO:0044444,GO:0044446,GO:0044464,GO:0044769,GO:0045851,GO:0046961,GO:0048878,GO:0050801,GO:0051179,GO:0051234,GO:0051452,GO:0051453,GO:0055044,GO:0055067,GO:0055080,GO:0055082,GO:0055085,GO:0065007,GO:0065008,GO:0071944,GO:0090662,GO:0098588,GO:0098655,GO:0098660,GO:0098662,GO:0098771,GO:0098796,GO:0098805,GO:0098852,GO:0099131,GO:0099132,GO:1902600
EggNOG free text desc.Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system
EggNOG OGs37N5I@33090,3GFHK@35493,4JI5I@91835,COG1527@1,KOG2957@2759
COG Functional cat.C
Best tax levelfabids
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002
Exons3
Model size384
Cds size384
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig120403.4209.1prot_M-pyrifera_M_contig120403.4209.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig120403 34..535 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815723.7795398-CDS-M-pyrifera_M_contig120403:33..841622815723.7795398-CDS-M-pyrifera_M_contig120403:33..84Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig120403 34..84 +
1692277539.1380792-CDS-M-pyrifera_M_contig120403:33..841692277539.1380792-CDS-M-pyrifera_M_contig120403:33..84Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig120403 34..84 +
1622815723.7927227-CDS-M-pyrifera_M_contig120403:117..2281622815723.7927227-CDS-M-pyrifera_M_contig120403:117..228Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig120403 118..228 +
1692277539.149367-CDS-M-pyrifera_M_contig120403:117..2281692277539.149367-CDS-M-pyrifera_M_contig120403:117..228Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig120403 118..228 +
1622815723.808181-CDS-M-pyrifera_M_contig120403:313..5351622815723.808181-CDS-M-pyrifera_M_contig120403:313..535Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig120403 314..535 +
1692277539.158278-CDS-M-pyrifera_M_contig120403:313..5351692277539.158278-CDS-M-pyrifera_M_contig120403:313..535Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig120403 314..535 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig120403.4209.1

>prot_M-pyrifera_M_contig120403.4209.1 ID=prot_M-pyrifera_M_contig120403.4209.1|Name=mRNA_M-pyrifera_M_contig120403.4209.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=128bp
APYFRDCLSRDALQDLAIEIIRNLLHKAYLEDFYRYCREELGGTTAETMS
ELLEFEADRRAITITQNCMGKDIGKDDREKLYCNFGRLYPEATEKLAKAS
DMDEVRAVLELPGYGDYKLVLEATGFGG
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mRNA from alignment at M-pyrifera_M_contig120403:34..535+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig120403.4209.1 ID=mRNA_M-pyrifera_M_contig120403.4209.1|Name=mRNA_M-pyrifera_M_contig120403.4209.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=502bp|location=Sequence derived from alignment at M-pyrifera_M_contig120403:34..535+ (Macrocystis pyrifera P11B4 male)
GCCCCCTACTTCCGCGACTGCCTGAGCAGGGACGCTCTGCAGGATCTCGC
CGTTGGCCCAGGAGGTATGGCCAACTCGGGAGAGATTGAGATCATCCGTA
ACCTTCTCCACAAGGCCTACCTCGAGGATTTCTACAGATATTGCCGCGAG
GAGCTCGGTGGCACTACCGCGGAGACGATGTCAGAACTTCTGGAGGTACG
ACTCTACCTGTTCTCCCCTATTCCACGCGTTGGTTATTTCTGTCGGTGCT
TTTTTGGGATGCGATCTGACGAGTGTTCAGTTTGAGGCAGACAGGCGTGC
CATCACCATCACGCAGAACTGCATGGGCAAGGACATCGGAAAGGACGACA
GGGAGAAGCTCTACTGCAACTTCGGTCGTCTCTACCCCGAGGCAACGGAG
AAGCTGGCCAAGGCCTCGGACATGGACGAGGTGCGTGCCGTGCTGGAGCT
GCCTGGCTACGGAGACTACAAGCTGGTGCTGGAGGCGACCGGCTTCGGCG
GT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig120403:34..535+

>mRNA_M-pyrifera_M_contig120403.4209.1 ID=mRNA_M-pyrifera_M_contig120403.4209.1|Name=mRNA_M-pyrifera_M_contig120403.4209.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=768bp|location=Sequence derived from alignment at M-pyrifera_M_contig120403:34..535+ (Macrocystis pyrifera P11B4 male)
GCCCCCTACTTCCGCGACTGCCTGAGCAGGGACGCTCTGCAGGATCTCGC
CGCCCCCTACTTCCGCGACTGCCTGAGCAGGGACGCTCTGCAGGATCTCG
CCATTGAGATCATCCGTAACCTTCTCCACAAGGCCTACCTCGAGGATTTC
TACAGATATTGCCGCGAGGAGCTCGGTGGCACTACCGCGGAGACGATGTC
AGAACTTCTGGAGATTGAGATCATCCGTAACCTTCTCCACAAGGCCTACC
TCGAGGATTTCTACAGATATTGCCGCGAGGAGCTCGGTGGCACTACCGCG
GAGACGATGTCAGAACTTCTGGAGTTTGAGGCAGACAGGCGTGCCATCAC
CATCACGCAGAACTGCATGGGCAAGGACATCGGAAAGGACGACAGGGAGA
AGCTCTACTGCAACTTCGGTCGTCTCTACCCCGAGGCAACGGAGAAGCTG
GCCAAGGCCTCGGACATGGACGAGGTGCGTGCCGTGCTGGAGCTGCCTGG
CTACGGAGACTACAAGCTGGTGCTGGAGGCGACCGGCTTCGGCGGTTTTG
AGGCAGACAGGCGTGCCATCACCATCACGCAGAACTGCATGGGCAAGGAC
ATCGGAAAGGACGACAGGGAGAAGCTCTACTGCAACTTCGGTCGTCTCTA
CCCCGAGGCAACGGAGAAGCTGGCCAAGGCCTCGGACATGGACGAGGTGC
GTGCCGTGCTGGAGCTGCCTGGCTACGGAGACTACAAGCTGGTGCTGGAG
GCGACCGGCTTCGGCGGT
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