mRNA_M-pyrifera_M_contig116874.3490.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig116874.3490.1
Unique NamemRNA_M-pyrifera_M_contig116874.3490.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig116874.3490.1 vs. uniprot
Match: A0A1X7VW56_AMPQE (Uncharacterized protein n=2 Tax=Amphimedon queenslandica TaxID=400682 RepID=A0A1X7VW56_AMPQE)

HSP 1 Score: 144 bits (363), Expect = 3.880e-40
Identity = 76/139 (54.68%), Postives = 101/139 (72.66%), Query Frame = 1 
Query:   13 LYRQGGMRSLLRGVPATIARDTSATAFYFVTYESVKKELIRTNTNPTLAT---LLAGGLAGVANWIVAIPIDTLKSNLQVSTTPGNYPHGIRSVWKEMRLNREPVTSLFRGLTPALLRAFPSNAACFLGYETAMNFLVR 420
            L+R+GG+RSL RG  ATI RD   +A YF  Y+ + + L  T+ + +L+    L AGG+AGVANWI+AIP D LKS  Q++ T G YP+GIRSV+KEM  N E +TSL++G+ PA++RAFP+NAACFLGYE A+  L R
Sbjct:  120 LFREGGIRSLYRGTMATILRDVPGSAAYFGVYQWILRSLTPTDGSTSLSPSRILFAGGMAGVANWIIAIPPDVLKSRYQIAPT-GKYPNGIRSVFKEMMQN-EGITSLYKGVGPAMIRAFPANAACFLGYEVAIKVLDR 256
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BLAST of mRNA_M-pyrifera_M_contig116874.3490.1 vs. uniprot
Match: A0A0L0FIY7_9EUKA (Uncharacterized protein n=1 Tax=Sphaeroforma arctica JP610 TaxID=667725 RepID=A0A0L0FIY7_9EUKA)

HSP 1 Score: 145 bits (366), Expect = 5.370e-40
Identity = 76/136 (55.88%), Postives = 99/136 (72.79%), Query Frame = 1 
Query:   13 LYRQGGMRSLLRGVPATIARDTSATAFYFVTYESVKKELIRTN-TNPTLA-TLLAGGLAGVANWIVAIPIDTLKSNLQVSTTPGNYPHGIRSVWKEMRLNREPVTSLFRGLTPALLRAFPSNAACFLGYETAMNFL 414
            LY  GGM+S++RG  AT  RD+ A+AFYF +YE +K+ L     + P +  TLLAGG AG+ NW +A+PIDTLKS LQV+   G YPHGIRSV++E+  N  P  +L+RG  P +LRAFP+NAACFLGYE+A+ FL
Sbjct:  177 LYGAGGMKSMMRGFTATAGRDSIASAFYFSSYEVLKQALTPAGESGPGIGGTLLAGGSAGIFNWAIALPIDTLKSKLQVAPD-GKYPHGIRSVFREVMQNEGP-RALYRGFVPVMLRAFPANAACFLGYESALKFL 310
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BLAST of mRNA_M-pyrifera_M_contig116874.3490.1 vs. uniprot
Match: A0A7S4IGQ0_9EUKA (Hypothetical protein n=1 Tax=Vannella sp. CB-2014 TaxID=1487602 RepID=A0A7S4IGQ0_9EUKA)

HSP 1 Score: 142 bits (357), Expect = 7.580e-39
Identity = 77/135 (57.04%), Postives = 97/135 (71.85%), Query Frame = 1 
Query:   13 LYRQGGMRSLLRGVPATIARDTSATAFYFVTYESVKKELIRTNTNPT-LATLLAGGLAGVANWIVAIPIDTLKSNLQVSTTPGNYPHGIRSVWKEMRLNREPVTSLFRGLTPALLRAFPSNAACFLGYETAMNFL 414
            L ++GG  SL RG  AT+ARD  A+AFYF TYE  KK+    +   T + TL AGG+AG+ NW+ AIPIDTLKS LQV+   G YPHGIRSV+ ++  N E  ++LFRGL P +LRAFP+NAACFLGYETA+  +
Sbjct:  159 LLKEGGASSLFRGFWATMARDGLASAFYFSTYEFCKKKFAGADGTVTPVGTLTAGGIAGMLNWLFAIPIDTLKSKLQVAPD-GKYPHGIRSVFVDVMKN-EGASALFRGLGPIMLRAFPANAACFLGYETAIKVI 291
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BLAST of mRNA_M-pyrifera_M_contig116874.3490.1 vs. uniprot
Match: A0A3P6TKI6_LITSI (Uncharacterized protein n=3 Tax=Onchocercidae TaxID=6296 RepID=A0A3P6TKI6_LITSI)

HSP 1 Score: 141 bits (356), Expect = 1.630e-38
Identity = 77/138 (55.80%), Postives = 95/138 (68.84%), Query Frame = 1 
Query:   13 LYRQGGMRSLLRGVPATIARDTSATAFYFVTYESVKK----ELIRTNTNPTLATLLAGGLAGVANWIVAIPIDTLKSNLQVSTTPGNYPHGIRSVWKEMRLNREPVTSLFRGLTPALLRAFPSNAACFLGYETAMNFL 414
            LY+QGG+RS+ RG  AT+ RD  A+  Y  TYE +KK    +    N NP L TL+AGGLAG ANW + IP D LKS LQ +   G YP GIRSV+KE+ L  E   +LF+G TP +LRAFP+NAACFLG+E A+NFL
Sbjct:  164 LYKQGGIRSIYRGSMATLLRDVPASGIYLATYEYLKKLFAGDSETKNLNP-LTTLVAGGLAGTANWSICIPPDVLKSRLQTAPE-GKYPEGIRSVFKEI-LREEGPKALFKGFTPVMLRAFPANAACFLGFELALNFL 298
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BLAST of mRNA_M-pyrifera_M_contig116874.3490.1 vs. uniprot
Match: A0A075A2W6_9TREM (Uncharacterized protein n=5 Tax=Opisthorchiidae TaxID=6196 RepID=A0A075A2W6_9TREM)

HSP 1 Score: 141 bits (355), Expect = 1.740e-38
Identity = 76/138 (55.07%), Postives = 98/138 (71.01%), Query Frame = 1 
Query:   13 LYRQGGMRSLLRGVPATIARDTSATAFYFVTYESVKKELIRTN-TNPTLA---TLLAGGLAGVANWIVAIPIDTLKSNLQVSTTPGNYPHGIRSVWKEMRLNREPVTSLFRGLTPALLRAFPSNAACFLGYETAMNFL 414
            LYR+GG+RSL RG  AT+ RD  A+  YF++YE +K  L +T  T   L+   TL AGG+AG+ NW+VAIP D LKS  Q S   G YP+GIRSV+ E+ + +E   +L+RG+TP LLRAFP+NAACFLGYE A+ FL
Sbjct:  161 LYREGGLRSLYRGTAATLLRDVPASGAYFLSYEWIKDALRKTGETGDELSVGKTLFAGGMAGIFNWLVAIPPDVLKSRYQ-SAPEGRYPNGIRSVFSEL-IAKEGFFALYRGVTPVLLRAFPANAACFLGYEVALKFL 296
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BLAST of mRNA_M-pyrifera_M_contig116874.3490.1 vs. uniprot
Match: A0A0R3S0T7_9BILA (Uncharacterized protein n=1 Tax=Elaeophora elaphi TaxID=1147741 RepID=A0A0R3S0T7_9BILA)

HSP 1 Score: 140 bits (352), Expect = 5.350e-38
Identity = 72/138 (52.17%), Postives = 95/138 (68.84%), Query Frame = 1 
Query:   13 LYRQGGMRSLLRGVPATIARDTSATAFYFVTYESVKKELIRTNTNPTLA---TLLAGGLAGVANWIVAIPIDTLKSNLQVSTTPGNYPHGIRSVWKEMRLNREPVTSLFRGLTPALLRAFPSNAACFLGYETAMNFLV 417
            LY+QGG+RS+ RG  AT+ RD  A+  Y  TYE +KK     NT+  L+   TL+AGGLAG+ANW + IP D LKS LQ ++  G YP+GIR V+KE+ +  E   +LF+G TP +LRAFP+NAACFLG+E A+ F  
Sbjct:  165 LYKQGGIRSIYRGSMATLLRDVPASGVYLATYEYLKKLFTGNNTSKNLSPLSTLVAGGLAGIANWSICIPPDVLKSRLQTASE-GKYPNGIRDVFKEV-MREEGPKALFKGFTPVMLRAFPANAACFLGFELALKFFT 300
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BLAST of mRNA_M-pyrifera_M_contig116874.3490.1 vs. uniprot
Match: A0A4Z2CTI8_SCHJA (Mitochondrial carnitine/acylcarnitine carrier protein n=1 Tax=Schistosoma japonicum TaxID=6182 RepID=A0A4Z2CTI8_SCHJA)

HSP 1 Score: 137 bits (346), Expect = 1.390e-37
Identity = 71/138 (51.45%), Postives = 97/138 (70.29%), Query Frame = 1 
Query:   13 LYRQGGMRSLLRGVPATIARDTSATAFYFVTYESVKKEL----IRTNTNPTLATLLAGGLAGVANWIVAIPIDTLKSNLQVSTTPGNYPHGIRSVWKEMRLNREPVTSLFRGLTPALLRAFPSNAACFLGYETAMNFL 414
            LY +GG+RS+ +G  AT+ RD  A+  YF++YE +K +L     ++N      TL AGG+AG+ NWIVAIP D LKS LQ S   G YP+GIRSV+ E+ + +E   +L++G+TP +LRAFP+NAACFLGYE A+ FL
Sbjct:  119 LYGKGGLRSIFKGTAATLLRDVPASGVYFLSYELIKDKLRNRHSKSNELSVSKTLFAGGMAGIFNWIVAIPPDVLKSRLQ-SAPEGLYPNGIRSVFSEL-IAKEGFLALYKGMTPVMLRAFPANAACFLGYEVALKFL 254
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BLAST of mRNA_M-pyrifera_M_contig116874.3490.1 vs. uniprot
Match: UPI000E6DA2D9 (congested-like trachea protein n=1 Tax=Hyposmocoma kahamanoa TaxID=1477025 RepID=UPI000E6DA2D9)

HSP 1 Score: 138 bits (348), Expect = 1.720e-37
Identity = 69/137 (50.36%), Postives = 96/137 (70.07%), Query Frame = 1 
Query:   13 LYRQGGMRSLLRGVPATIARDTSATAFYFVTYESVKKELIRTNTNPT---LATLLAGGLAGVANWIVAIPIDTLKSNLQVSTTPGNYPHGIRSVWKEMRLNREPVTSLFRGLTPALLRAFPSNAACFLGYETAMNFL 414
            LY +GG+RS+ +G  ATI RD  A+  YF+TYE VK+ L+  N       L T+ AGG AG+ANW+V +P D LKS LQ +   G YP+G+R V+K++  N  P T+L++G+TP ++RAFP+NAACFLG+E A+ FL
Sbjct:  157 LYVEGGIRSIYKGSIATICRDVPASGMYFMTYEWVKEALVPANATDQYKMLITIFAGGCAGIANWLVGMPADVLKSRLQTAPE-GTYPNGMRDVFKQLMKNEGP-TALYKGVTPVMIRAFPANAACFLGFELAVKFL 291
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BLAST of mRNA_M-pyrifera_M_contig116874.3490.1 vs. uniprot
Match: A0A1D1UMG2_RAMVA (Uncharacterized protein n=1 Tax=Ramazzottius varieornatus TaxID=947166 RepID=A0A1D1UMG2_RAMVA)

HSP 1 Score: 139 bits (349), Expect = 2.100e-37
Identity = 70/138 (50.72%), Postives = 96/138 (69.57%), Query Frame = 1 
Query:   13 LYRQGGMRSLLRGVPATIARDTSATAFYFVTYESVKKELIRTNTNPT----LATLLAGGLAGVANWIVAIPIDTLKSNLQVSTTPGNYPHGIRSVWKEMRLNREPVTSLFRGLTPALLRAFPSNAACFLGYETAMNFL 414
            LY +GG++S+ RG  AT+ RD  A+  YF++YE +KK +     +P+    L ++LAGG AG+ NWIVA+P D LKS LQ +   G YP+G+R V++E+ +  E    LF+G+TP +LRAFPSNAACFLGYE AM FL
Sbjct:  180 LYAEGGLKSVFRGTGATLLRDVPASGVYFMSYEWLKKAVTPPGEDPSKINPLRSILAGGTAGILNWIVALPPDVLKSRLQTAAE-GTYPNGMRDVFREL-MREEGPRGLFKGITPVMLRAFPSNAACFLGYEVAMKFL 315
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BLAST of mRNA_M-pyrifera_M_contig116874.3490.1 vs. uniprot
Match: G4VCL1_SCHMA (Mitochondrial carnitine/acylcarnitine carrier protein-related n=3 Tax=Schistosoma TaxID=6181 RepID=G4VCL1_SCHMA)

HSP 1 Score: 138 bits (347), Expect = 2.650e-37
Identity = 71/138 (51.45%), Postives = 96/138 (69.57%), Query Frame = 1 
Query:   13 LYRQGGMRSLLRGVPATIARDTSATAFYFVTYESVKKEL----IRTNTNPTLATLLAGGLAGVANWIVAIPIDTLKSNLQVSTTPGNYPHGIRSVWKEMRLNREPVTSLFRGLTPALLRAFPSNAACFLGYETAMNFL 414
            LYR+GG+RS+ +G  AT+ RD  A+  YF++YE +K  L     + N      TL AGG+AG+ NW++AIP D LKS LQ S + G YP+GIRSV+ E+ + +E    L+RG+TP +LRAFP+NAACFLGYE A+ FL
Sbjct:  160 LYREGGIRSIFKGTAATLLRDVPASGVYFLSYEVMKDALRNPHSKNNELSVGKTLFAGGMAGIFNWLIAIPPDVLKSRLQ-SASEGVYPNGIRSVFSEL-IAKEGFLGLYRGMTPVMLRAFPANAACFLGYEVALKFL 295
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig116874.3490.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A1X7VW56_AMPQE3.880e-4054.68Uncharacterized protein n=2 Tax=Amphimedon queensl... [more]
A0A0L0FIY7_9EUKA5.370e-4055.88Uncharacterized protein n=1 Tax=Sphaeroforma arcti... [more]
A0A7S4IGQ0_9EUKA7.580e-3957.04Hypothetical protein n=1 Tax=Vannella sp. CB-2014 ... [more]
A0A3P6TKI6_LITSI1.630e-3855.80Uncharacterized protein n=3 Tax=Onchocercidae TaxI... [more]
A0A075A2W6_9TREM1.740e-3855.07Uncharacterized protein n=5 Tax=Opisthorchiidae Ta... [more]
A0A0R3S0T7_9BILA5.350e-3852.17Uncharacterized protein n=1 Tax=Elaeophora elaphi ... [more]
A0A4Z2CTI8_SCHJA1.390e-3751.45Mitochondrial carnitine/acylcarnitine carrier prot... [more]
UPI000E6DA2D91.720e-3750.36congested-like trachea protein n=1 Tax=Hyposmocoma... [more]
A0A1D1UMG2_RAMVA2.100e-3750.72Uncharacterized protein n=1 Tax=Ramazzottius varie... [more]
G4VCL1_SCHMA2.650e-3751.45Mitochondrial carnitine/acylcarnitine carrier prot... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig116874contigM-pyrifera_M_contig116874:93..527 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeOpisthokonta
Seed ortholog score147.1
Seed ortholog evalue6.2e-33
Seed eggNOG ortholog72019.SARC_11064T0
Preferred nameSLC25A20
KEGG koko:K15109
KEGG TC2.A.29.8
KEGG Pathwayko04714,map04714
Hectar predicted targeting categorymitochondrion
GOsGO:0000902,GO:0000904,GO:0002064,GO:0003382,GO:0003674,GO:0005215,GO:0005476,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0005740,GO:0005743,GO:0005829,GO:0006810,GO:0006811,GO:0006812,GO:0006820,GO:0006839,GO:0006844,GO:0006853,GO:0006869,GO:0006873,GO:0006875,GO:0007275,GO:0007424,GO:0008150,GO:0008324,GO:0008519,GO:0009653,GO:0009888,GO:0009987,GO:0010876,GO:0010960,GO:0010961,GO:0015075,GO:0015095,GO:0015101,GO:0015226,GO:0015227,GO:0015291,GO:0015297,GO:0015318,GO:0015651,GO:0015693,GO:0015695,GO:0015696,GO:0015697,GO:0015711,GO:0015718,GO:0015838,GO:0015849,GO:0015879,GO:0015908,GO:0015909,GO:0016020,GO:0016021,GO:0016043,GO:0019725,GO:0019866,GO:0022804,GO:0022857,GO:0022890,GO:0030001,GO:0030003,GO:0030154,GO:0030855,GO:0031090,GO:0031224,GO:0031966,GO:0031967,GO:0031975,GO:0032365,GO:0032501,GO:0032502,GO:0032989,GO:0033036,GO:0034220,GO:0035002,GO:0042044,GO:0042045,GO:0042592,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044422,GO:0044424,GO:0044425,GO:0044429,GO:0044444,GO:0044446,GO:0044464,GO:0045016,GO:0046873,GO:0046907,GO:0046942,GO:0048468,GO:0048731,GO:0048856,GO:0048869,GO:0048878,GO:0050801,GO:0051179,GO:0051181,GO:0051234,GO:0051641,GO:0051649,GO:0055065,GO:0055080,GO:0055082,GO:0055085,GO:0060429,GO:0060541,GO:0065007,GO:0065008,GO:0070633,GO:0070838,GO:0071702,GO:0071705,GO:0071840,GO:0072337,GO:0072349,GO:0072488,GO:0072503,GO:0072507,GO:0072511,GO:0098655,GO:0098656,GO:0098660,GO:0098662,GO:0098771,GO:1902001,GO:1902603,GO:1902616,GO:1903825,GO:1903830,GO:1905039,GO:1990542,GO:1990616
EggNOG free text desc.carnitine:acyl carnitine antiporter activity
EggNOG OGs38BD7@33154,KOG0758@1,KOG0758@2759
COG Functional cat.C
Best tax levelOpisthokonta
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko02000
Exons1
Model size435
Cds size435
Stop1
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815685.3749955-CDS-M-pyrifera_M_contig116874:92..5271622815685.3749955-CDS-M-pyrifera_M_contig116874:92..527Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig116874 93..527 +
1692277521.0647247-CDS-M-pyrifera_M_contig116874:92..5271692277521.0647247-CDS-M-pyrifera_M_contig116874:92..527Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig116874 93..527 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig116874.3490.1prot_M-pyrifera_M_contig116874.3490.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig116874 93..527 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig116874.3490.1

>prot_M-pyrifera_M_contig116874.3490.1 ID=prot_M-pyrifera_M_contig116874.3490.1|Name=mRNA_M-pyrifera_M_contig116874.3490.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=145bp
MCAALYRQGGMRSLLRGVPATIARDTSATAFYFVTYESVKKELIRTNTNP
TLATLLAGGLAGVANWIVAIPIDTLKSNLQVSTTPGNYPHGIRSVWKEMR
LNREPVTSLFRGLTPALLRAFPSNAACFLGYETAMNFLVRDRAQ*
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mRNA from alignment at M-pyrifera_M_contig116874:93..527+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig116874.3490.1 ID=mRNA_M-pyrifera_M_contig116874.3490.1|Name=mRNA_M-pyrifera_M_contig116874.3490.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=435bp|location=Sequence derived from alignment at M-pyrifera_M_contig116874:93..527+ (Macrocystis pyrifera P11B4 male)
ATGTGTGCCGCCTTGTACCGTCAAGGGGGCATGAGGTCATTGCTCAGAGG
AGTCCCTGCGACTATTGCAAGGGACACTTCAGCGACTGCGTTCTATTTTG
TTACATATGAGTCTGTGAAGAAGGAACTGATCCGCACCAACACAAACCCA
ACCCTCGCCACATTGCTCGCAGGTGGGCTGGCAGGTGTGGCGAACTGGAT
CGTTGCAATCCCCATCGACACTCTCAAGTCCAACTTGCAGGTCTCTACCA
CGCCTGGCAACTACCCCCACGGCATCAGGAGTGTGTGGAAGGAAATGCGG
CTCAACCGAGAGCCGGTGACATCCCTGTTCCGTGGGCTGACACCTGCATT
GCTGCGGGCCTTCCCTAGCAACGCGGCGTGCTTCCTCGGCTACGAAACTG
CCATGAATTTCCTGGTGAGGGACAGAGCACAGTAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig116874:93..527+

>mRNA_M-pyrifera_M_contig116874.3490.1 ID=mRNA_M-pyrifera_M_contig116874.3490.1|Name=mRNA_M-pyrifera_M_contig116874.3490.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=870bp|location=Sequence derived from alignment at M-pyrifera_M_contig116874:93..527+ (Macrocystis pyrifera P11B4 male)
ATGTGTGCCGCCTTGTACCGTCAAGGGGGCATGAGGTCATTGCTCAGAGG
AGTCCCTGCGACTATTGCAAGGGACACTTCAGCGACTGCGTTCTATTTTG
TTACATATGAGTCTGTGAAGAAGGAACTGATCCGCACCAACACAAACCCA
ACCCTCGCCACATTGCTCGCAGGTGGGCTGGCAGGTGTGGCGAACTGGAT
CGTTGCAATCCCCATCGACACTCTCAAGTCCAACTTGCAGGTCTCTACCA
CGCCTGGCAACTACCCCCACGGCATCAGGAGTGTGTGGAAGGAAATGCGG
CTCAACCGAGAGCCGGTGACATCCCTGTTCCGTGGGCTGACACCTGCATT
GCTGCGGGCCTTCCCTAGCAACGCGGCGTGCTTCCTCGGCTACGAAACTG
CCATGAATTTCCTGGTGAGGGACAGAGCACAGTAAATGTGTGCCGCCTTG
TACCGTCAAGGGGGCATGAGGTCATTGCTCAGAGGAGTCCCTGCGACTAT
TGCAAGGGACACTTCAGCGACTGCGTTCTATTTTGTTACATATGAGTCTG
TGAAGAAGGAACTGATCCGCACCAACACAAACCCAACCCTCGCCACATTG
CTCGCAGGTGGGCTGGCAGGTGTGGCGAACTGGATCGTTGCAATCCCCAT
CGACACTCTCAAGTCCAACTTGCAGGTCTCTACCACGCCTGGCAACTACC
CCCACGGCATCAGGAGTGTGTGGAAGGAAATGCGGCTCAACCGAGAGCCG
GTGACATCCCTGTTCCGTGGGCTGACACCTGCATTGCTGCGGGCCTTCCC
TAGCAACGCGGCGTGCTTCCTCGGCTACGAAACTGCCATGAATTTCCTGG
TGAGGGACAGAGCACAGTAA
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