mRNA_M-pyrifera_M_contig112748.2649.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig112748.2649.1
Unique NamemRNA_M-pyrifera_M_contig112748.2649.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig112748.2649.1 vs. uniprot
Match: A0A152A320_9MYCE (Cullin A n=1 Tax=Tieghemostelium lacteum TaxID=361077 RepID=A0A152A320_9MYCE)

HSP 1 Score: 221 bits (564), Expect = 4.220e-65
Identity = 115/171 (67.25%), Postives = 131/171 (76.61%), Query Frame = 1 
Query:    1 MTIFKYIEEKDVFMTFYSKALAKRLIQGLSASEDLEGSMISKLKSACGFEYTSKLQRMFTDMTVSRDLEEAFRER--NSGSEVTLGCDFSVLVLATGSWPLTAPTTGFEIPKALAVCEAEFKSFYCKKHSGRKLSWLHQHCKGELKCVEPLGASKMLYTFQCSTYQMGVLL 507
            MTIFKYIE+KDVF  FYSK LAKRLI G S SEDLEG+MI KLKS CG+EYTSKLQRMFTD+++SRDL E F      +G+ VTLG DFSV+VLATGSWPL  P T F IPK L VCE  F++FY  ++SGRKL+WLH   KGELK  + L  SK  YT QCSTYQ+GVLL
Sbjct:  443 MTIFKYIEDKDVFQDFYSKMLAKRLIHGTSTSEDLEGTMIGKLKSTCGYEYTSKLQRMFTDISLSRDLLERFNSHIEQNGNGVTLGMDFSVMVLATGSWPLQPPNTNFSIPKELQVCEQLFQNFYQHQYSGRKLNWLHHLSKGELK-TKYLPTSKKGYTLQCSTYQIGVLL 612
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BLAST of mRNA_M-pyrifera_M_contig112748.2649.1 vs. uniprot
Match: A0A2P6NSZ7_9EUKA (Cullin A n=1 Tax=Planoprotostelium fungivorum TaxID=1890364 RepID=A0A2P6NSZ7_9EUKA)

HSP 1 Score: 215 bits (547), Expect = 1.090e-62
Identity = 108/169 (63.91%), Postives = 125/169 (73.96%), Query Frame = 1 
Query:    1 MTIFKYIEEKDVFMTFYSKALAKRLIQGLSASEDLEGSMISKLKSACGFEYTSKLQRMFTDMTVSRDLEEAFRERNSGSEVTLGCDFSVLVLATGSWPLTAPTTGFEIPKALAVCEAEFKSFYCKKHSGRKLSWLHQHCKGELKCVEPLGASKMLYTFQCSTYQMGVLL 507
            M +FKYIE+KDVF TFYSK LAKRLI   SASEDLEG MI KLKS CG+EYTSKLQRMFTDM++SRDL + F+      +  LG DFS+LVLATGSWPL  P+T F +PK L  CE  F  FY  +H+GRKL+WLHQ  KGE+K    L ++K  YT Q STYQMGVLL
Sbjct:  441 MVVFKYIEDKDVFQTFYSKMLAKRLIHATSASEDLEGVMIGKLKSTCGYEYTSKLQRMFTDMSLSRDLLDRFKTTIEEKQTNLGVDFSILVLATGSWPLQPPSTNFTVPKELQTCEQLFLKFYSAQHNGRKLNWLHQLSKGEMK-TSYLTSNKAGYTLQASTYQMGVLL 608
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BLAST of mRNA_M-pyrifera_M_contig112748.2649.1 vs. uniprot
Match: F0Z8D7_DICPU (Cullin n=1 Tax=Dictyostelium purpureum TaxID=5786 RepID=F0Z8D7_DICPU)

HSP 1 Score: 211 bits (538), Expect = 2.300e-61
Identity = 111/170 (65.29%), Postives = 125/170 (73.53%), Query Frame = 1 
Query:    1 MTIFKYIEEKDVFMTFYSKALAKRLIQGLSASEDLEGSMISKLKSACGFEYTSKLQRMFTDMTVSRDLEEAFRERNSGSE-VTLGCDFSVLVLATGSWPLTAPTTGFEIPKALAVCEAEFKSFYCKKHSGRKLSWLHQHCKGELKCVEPLGASKMLYTFQCSTYQMGVLL 507
            M +FKYIE+KDVF  FYSK LAKRLI G S SEDLEG+MI KLKS CG+EYTSKLQRMFTDM++SR+L + F       E   LG DFSVLVLATGSWPL  P+T F IPK L  CE  F+ FY  +HSGRKL+WLH   KGELK  + L  SK  YT QCSTYQ+GVLL
Sbjct:  443 MIVFKYIEDKDVFQDFYSKMLAKRLIHGTSTSEDLEGTMIGKLKSTCGYEYTSKLQRMFTDMSLSRELLDRFNNHIEQVERQALGIDFSVLVLATGSWPLQPPSTNFSIPKELQGCEQLFQKFYQNQHSGRKLNWLHHLSKGELK-TKYLQTSKSGYTLQCSTYQIGVLL 611
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BLAST of mRNA_M-pyrifera_M_contig112748.2649.1 vs. uniprot
Match: CUL1_DICDI (Cullin-1 n=2 Tax=Dictyosteliaceae TaxID=2058185 RepID=CUL1_DICDI)

HSP 1 Score: 211 bits (536), Expect = 4.290e-61
Identity = 110/170 (64.71%), Postives = 125/170 (73.53%), Query Frame = 1 
Query:    1 MTIFKYIEEKDVFMTFYSKALAKRLIQGLSASEDLEGSMISKLKSACGFEYTSKLQRMFTDMTVSRDLEEAFRERNSGSE-VTLGCDFSVLVLATGSWPLTAPTTGFEIPKALAVCEAEFKSFYCKKHSGRKLSWLHQHCKGELKCVEPLGASKMLYTFQCSTYQMGVLL 507
            M +FKYIE+KDVF  FYSK LAKRLI G S SEDLEG+MI KLKS CG+EYTSKLQRMFTDM++SR+L + F       E  +L  DFSVLVLATGSWPL  P+T F IPK L  CE  F+ FY  +HSGRKL+WLH   KGELK  + L  SK  YT QCSTYQ+GVLL
Sbjct:  441 MIVFKYIEDKDVFQDFYSKMLAKRLIHGTSTSEDLEGTMIGKLKSTCGYEYTSKLQRMFTDMSLSRELLDRFNNHIEQVERSSLNIDFSVLVLATGSWPLQPPSTNFSIPKELQACEQLFQKFYQNQHSGRKLNWLHHLSKGELK-TKYLQTSKSGYTLQCSTYQIGVLL 609
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BLAST of mRNA_M-pyrifera_M_contig112748.2649.1 vs. uniprot
Match: D3B2W9_POLPP (Cullin A n=1 Tax=Polysphondylium pallidum (strain ATCC 26659 / Pp 5 / PN500) TaxID=670386 RepID=D3B2W9_POLPP)

HSP 1 Score: 209 bits (531), Expect = 1.960e-60
Identity = 107/169 (63.31%), Postives = 123/169 (72.78%), Query Frame = 1 
Query:    1 MTIFKYIEEKDVFMTFYSKALAKRLIQGLSASEDLEGSMISKLKSACGFEYTSKLQRMFTDMTVSRDLEEAFRERNSGSEVTLGCDFSVLVLATGSWPLTAPTTGFEIPKALAVCEAEFKSFYCKKHSGRKLSWLHQHCKGELKCVEPLGASKMLYTFQCSTYQMGVLL 507
            M +FKYIE+KDVF  FYSK LAKRLI G S SEDLEG MI KLKS CG+EYTSKLQRMFTDM++SR+L + F +         G DFSVLVLATGSWPL  P T F IPK L  CE  F+ FY  ++SGRKL+WLH   KGELK  + L ++K  YT QCSTYQ+GVLL
Sbjct:  441 MIVFKYIEDKDVFQDFYSKMLAKRLIHGTSTSEDLEGVMIGKLKSTCGYEYTSKLQRMFTDMSLSRELLDRFNQHLEEQSALGGIDFSVLVLATGSWPLQPPATNFSIPKELQACEQLFQKFYQVQYSGRKLNWLHHLSKGELK-TKYLPSNKSGYTLQCSTYQIGVLL 608
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BLAST of mRNA_M-pyrifera_M_contig112748.2649.1 vs. uniprot
Match: UPI0006449647 (hypothetical protein n=1 Tax=Acytostelium subglobosum LB1 TaxID=1410327 RepID=UPI0006449647)

HSP 1 Score: 208 bits (529), Expect = 4.570e-60
Identity = 110/171 (64.33%), Postives = 125/171 (73.10%), Query Frame = 1 
Query:    1 MTIFKYIEEKDVFMTFYSKALAKRLIQGLSASEDLEGSMISKLKSACGFEYTSKLQRMFTDMTVSRDLEEAFRERNSGSEVTL--GCDFSVLVLATGSWPLTAPTTGFEIPKALAVCEAEFKSFYCKKHSGRKLSWLHQHCKGELKCVEPLGASKMLYTFQCSTYQMGVLL 507
            MT+FKYIE+KDVF  FYSK LAKRLI G S SEDLEG MI KLKS CG+EYTSKLQRMFTDM++SR+L + F       E T   G DFSVLVLATGSWPL  P T F IPK L  CE  F+ FY  ++SGRKL+WLH   KGELK  + L ++K  YT QCSTYQ+GVLL
Sbjct:  446 MTVFKYIEDKDVFQDFYSKMLAKRLIHGTSTSEDLEGVMIGKLKSTCGYEYTSKLQRMFTDMSLSRELLDRFNTHVEQVEQTTLGGIDFSVLVLATGSWPLQPPATNFSIPKELQSCEQLFQKFYQVQYSGRKLNWLHHLSKGELK-TKYLPSNKTGYTLQCSTYQIGVLL 615
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BLAST of mRNA_M-pyrifera_M_contig112748.2649.1 vs. uniprot
Match: F4Q7P4_CAVFA (Cullin A n=1 Tax=Cavenderia fasciculata (strain SH3) TaxID=1054147 RepID=F4Q7P4_CAVFA)

HSP 1 Score: 207 bits (528), Expect = 6.570e-60
Identity = 108/172 (62.79%), Postives = 127/172 (73.84%), Query Frame = 1 
Query:    1 MTIFKYIEEKDVFMTFYSKALAKRLIQGLSASEDLEGSMISKLKSACGFEYTSKLQRMFTDMTVSRDLEEAFR---ERNSGSEVTLGCDFSVLVLATGSWPLTAPTTGFEIPKALAVCEAEFKSFYCKKHSGRKLSWLHQHCKGELKCVEPLGASKMLYTFQCSTYQMGVLL 507
            M +FKYIE+KDVF  FYSK LAKRLI G S SEDLEG MI KLKS CG+EYTSKLQRMFTDM++SRDL E F    E+++   +  G DFSVLVLATGSWPL  P T F IPK L  CE  F+ FY  ++SGRKL+WLH   KGELK  + L ++K  Y+ QCSTYQ+G+LL
Sbjct:  446 MIVFKYIEDKDVFQDFYSKMLAKRLIHGTSTSEDLEGVMIGKLKSTCGYEYTSKLQRMFTDMSLSRDLLERFHRHLEQDNQQVLLGGIDFSVLVLATGSWPLQPPATNFSIPKELQACEQLFQKFYQIQYSGRKLNWLHHLSKGELK-TKYLSSNKSGYSLQCSTYQIGILL 616
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BLAST of mRNA_M-pyrifera_M_contig112748.2649.1 vs. uniprot
Match: A0A2Z6SHW6_9GLOM (CULLIN_2 domain-containing protein n=1 Tax=Rhizophagus clarus TaxID=94130 RepID=A0A2Z6SHW6_9GLOM)

HSP 1 Score: 202 bits (515), Expect = 3.540e-59
Identity = 107/169 (63.31%), Postives = 121/169 (71.60%), Query Frame = 1 
Query:    1 MTIFKYIEEKDVFMTFYSKALAKRLIQGLSASEDLEGSMISKLKSACGFEYTSKLQRMFTDMTVSRDLEEAFRERNSGSEVTLGCDFSVLVLATGSWPLTAPTTGFEIPKALAVCEAEFKSFYCKKHSGRKLSWLHQHCKGELKCVEPLGASKMLYTFQCSTYQMGVLL 507
            MT+FKY+E+KDVF  FYSK LAKRL+ G SASED E SMISKLK ACGFEYTSKLQRMFTDM +S+DL + F++R S        DFSVLVL T SWPL   TT F IP+ +     +FK FY  KHSGRKL+WL    KGELK    L ASK  YTFQ STYQMG+LL
Sbjct:  269 MTVFKYVEDKDVFQKFYSKMLAKRLVNGTSASEDAESSMISKLKEACGFEYTSKLQRMFTDMGLSKDLNDQFKDRFSADAGDQQVDFSVLVLGTASWPLQPSTTNFIIPEEVERTFQKFKMFYQNKHSGRKLNWLFHLSKGELK-TNYLKASKTGYTFQVSTYQMGILL 436
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BLAST of mRNA_M-pyrifera_M_contig112748.2649.1 vs. uniprot
Match: A0A397U440_9GLOM (Cullin n=1 Tax=Gigaspora rosea TaxID=44941 RepID=A0A397U440_9GLOM)

HSP 1 Score: 205 bits (521), Expect = 5.940e-59
Identity = 109/169 (64.50%), Postives = 122/169 (72.19%), Query Frame = 1 
Query:    1 MTIFKYIEEKDVFMTFYSKALAKRLIQGLSASEDLEGSMISKLKSACGFEYTSKLQRMFTDMTVSRDLEEAFRERNSGSEVTLGCDFSVLVLATGSWPLTAPTTGFEIPKALAVCEAEFKSFYCKKHSGRKLSWLHQHCKGELKCVEPLGASKMLYTFQCSTYQMGVLL 507
            MT+FKY+E+KDVF  FYSK LAKRL+   SASED E SMISKLK ACGFEYTSKLQRMFTDM +S+DL + F+ER SG E   G DFSVLVL T SWPL+  TT F IP+ +      FK FY  KHSGRKL+WL    KGELK     GA K  YTFQ STYQMG+LL
Sbjct:  447 MTVFKYVEDKDVFQKFYSKMLAKRLVHSTSASEDAESSMISKLKEACGFEYTSKLQRMFTDMGLSKDLNDQFKERTSG-ETDNGVDFSVLVLGTASWPLSPSTTSFNIPEEVEKTFQRFKLFYQNKHSGRKLNWLFHLSKGELKTNYCKGA-KTGYTFQVSTYQMGILL 613
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BLAST of mRNA_M-pyrifera_M_contig112748.2649.1 vs. uniprot
Match: A0A433QR91_9FUNG (Cullin family-domain-containing protein n=1 Tax=Jimgerdemannia flammicorona TaxID=994334 RepID=A0A433QR91_9FUNG)

HSP 1 Score: 197 bits (502), Expect = 1.860e-58
Identity = 102/171 (59.65%), Postives = 121/171 (70.76%), Query Frame = 1 
Query:    1 MTIFKYIEEKDVFMTFYSKALAKRLIQGLSASEDLEGSMISKLKSACGFEYTSKLQRMFTDMTVSRDLEEAFRER--NSGSEVTLGCDFSVLVLATGSWPLTAPTTGFEIPKALAVCEAEFKSFYCKKHSGRKLSWLHQHCKGELKCVEPLGASKMLYTFQCSTYQMGVLL 507
            MT+FKY+E+KDVF  FYSK LAKRL+ G SAS+D E SMISKLK ACGFEYTSKLQRMFTDM +S++L + F++R   +     L  DF++LVL  GSWPLTAPTT F IP  +      F+ FY  KHSGRKL+WL Q  K ELK    L A+K  YT Q S YQMG+LL
Sbjct:  129 MTVFKYVEDKDVFQKFYSKMLAKRLVNGTSASDDAEASMISKLKEACGFEYTSKLQRMFTDMALSKELNDQFKDRMQQTHDSSDLSVDFTILVLGAGSWPLTAPTTPFNIPDDIVKTYDRFQKFYQAKHSGRKLNWLFQLSKAELK-TNYLKAAKTGYTLQVSAYQMGILL 298
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig112748.2649.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A152A320_9MYCE4.220e-6567.25Cullin A n=1 Tax=Tieghemostelium lacteum TaxID=361... [more]
A0A2P6NSZ7_9EUKA1.090e-6263.91Cullin A n=1 Tax=Planoprotostelium fungivorum TaxI... [more]
F0Z8D7_DICPU2.300e-6165.29Cullin n=1 Tax=Dictyostelium purpureum TaxID=5786 ... [more]
CUL1_DICDI4.290e-6164.71Cullin-1 n=2 Tax=Dictyosteliaceae TaxID=2058185 Re... [more]
D3B2W9_POLPP1.960e-6063.31Cullin A n=1 Tax=Polysphondylium pallidum (strain ... [more]
UPI00064496474.570e-6064.33hypothetical protein n=1 Tax=Acytostelium subglobo... [more]
F4Q7P4_CAVFA6.570e-6062.79Cullin A n=1 Tax=Cavenderia fasciculata (strain SH... [more]
A0A2Z6SHW6_9GLOM3.540e-5963.31CULLIN_2 domain-containing protein n=1 Tax=Rhizoph... [more]
A0A397U440_9GLOM5.940e-5964.50Cullin n=1 Tax=Gigaspora rosea TaxID=44941 RepID=A... [more]
A0A433QR91_9FUNG1.860e-5859.65Cullin family-domain-containing protein n=1 Tax=Ji... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig112748contigM-pyrifera_M_contig112748:2..508 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score212.2
Seed ortholog evalue1.8e-52
Seed eggNOG ortholog5786.XP_003283690.1
KEGG koko:K03347
KEGG Pathwayko04110,ko04111,ko04114,ko04120,ko04141,ko04310,ko04340,ko04341,ko04350,ko04710,ko05168,ko05200,map04110,map04111,map04114,map04120,map04141,map04310,map04340,map04341,map04350,map04710,map05168,map05200
KEGG ModuleM00379,M00380,M00381,M00382,M00387,M00407,M00411
Hectar predicted targeting categoryother localisation
GOsGO:0000082,GO:0000151,GO:0000278,GO:0003674,GO:0003824,GO:0004842,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0006464,GO:0006807,GO:0006935,GO:0007049,GO:0008150,GO:0008152,GO:0009605,GO:0009987,GO:0016567,GO:0016740,GO:0019005,GO:0019538,GO:0019787,GO:0019899,GO:0022402,GO:0030154,GO:0030587,GO:0031461,GO:0031625,GO:0032446,GO:0032502,GO:0032991,GO:0036211,GO:0040011,GO:0042221,GO:0042330,GO:0043170,GO:0043412,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044389,GO:0044424,GO:0044464,GO:0044770,GO:0044772,GO:0044843,GO:0048856,GO:0048869,GO:0050896,GO:0051703,GO:0051704,GO:0070647,GO:0071704,GO:0090702,GO:0099120,GO:0140096,GO:1901564,GO:1902494,GO:1903047,GO:1990234
EggNOG free text desc.the cullin
EggNOG OGs3X8GE@554915,COG5647@1,KOG2166@2759
COG Functional cat.O
Best tax levelAmoebozoa
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko04121
Exons1
Model size507
Cds size507
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815640.9965844-CDS-M-pyrifera_M_contig112748:1..5081622815640.9965844-CDS-M-pyrifera_M_contig112748:1..508Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig112748 2..508 -
1692277500.791522-CDS-M-pyrifera_M_contig112748:1..5081692277500.791522-CDS-M-pyrifera_M_contig112748:1..508Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig112748 2..508 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig112748.2649.1prot_M-pyrifera_M_contig112748.2649.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig112748 2..508 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig112748.2649.1

>prot_M-pyrifera_M_contig112748.2649.1 ID=prot_M-pyrifera_M_contig112748.2649.1|Name=mRNA_M-pyrifera_M_contig112748.2649.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=169bp
MTIFKYIEEKDVFMTFYSKALAKRLIQGLSASEDLEGSMISKLKSACGFE
YTSKLQRMFTDMTVSRDLEEAFRERNSGSEVTLGCDFSVLVLATGSWPLT
APTTGFEIPKALAVCEAEFKSFYCKKHSGRKLSWLHQHCKGELKCVEPLG
ASKMLYTFQCSTYQMGVLL
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mRNA from alignment at M-pyrifera_M_contig112748:2..508-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig112748.2649.1 ID=mRNA_M-pyrifera_M_contig112748.2649.1|Name=mRNA_M-pyrifera_M_contig112748.2649.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=507bp|location=Sequence derived from alignment at M-pyrifera_M_contig112748:2..508- (Macrocystis pyrifera P11B4 male)
ATGACGATCTTCAAGTACATCGAGGAGAAGGATGTGTTCATGACGTTCTA
CTCGAAGGCCCTGGCGAAGCGACTCATCCAGGGCCTGAGTGCGTCGGAGG
ACCTGGAGGGCAGCATGATCAGCAAACTCAAGTCTGCGTGTGGGTTCGAG
TACACTTCGAAGCTCCAACGGATGTTCACAGACATGACAGTAAGCAGAGA
CCTCGAGGAAGCGTTCAGAGAGCGAAACTCTGGAAGTGAAGTGACATTGG
GCTGTGACTTCAGTGTTCTCGTCCTGGCCACCGGCTCTTGGCCTCTCACC
GCACCCACGACAGGATTTGAGATCCCCAAGGCTCTCGCTGTGTGTGAGGC
AGAGTTCAAGTCATTCTATTGCAAGAAACACTCTGGGAGAAAGCTCAGTT
GGCTCCACCAACACTGCAAGGGAGAGCTGAAGTGTGTGGAGCCCTTGGGA
GCCTCCAAAATGCTCTATACCTTCCAATGCTCCACCTACCAGATGGGTGT
GCTCCTC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig112748:2..508-

>mRNA_M-pyrifera_M_contig112748.2649.1 ID=mRNA_M-pyrifera_M_contig112748.2649.1|Name=mRNA_M-pyrifera_M_contig112748.2649.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1014bp|location=Sequence derived from alignment at M-pyrifera_M_contig112748:2..508- (Macrocystis pyrifera P11B4 male)
ATGACGATCTTCAAGTACATCGAGGAGAAGGATGTGTTCATGACGTTCTA
CTCGAAGGCCCTGGCGAAGCGACTCATCCAGGGCCTGAGTGCGTCGGAGG
ACCTGGAGGGCAGCATGATCAGCAAACTCAAGTCTGCGTGTGGGTTCGAG
TACACTTCGAAGCTCCAACGGATGTTCACAGACATGACAGTAAGCAGAGA
CCTCGAGGAAGCGTTCAGAGAGCGAAACTCTGGAAGTGAAGTGACATTGG
GCTGTGACTTCAGTGTTCTCGTCCTGGCCACCGGCTCTTGGCCTCTCACC
GCACCCACGACAGGATTTGAGATCCCCAAGGCTCTCGCTGTGTGTGAGGC
AGAGTTCAAGTCATTCTATTGCAAGAAACACTCTGGGAGAAAGCTCAGTT
GGCTCCACCAACACTGCAAGGGAGAGCTGAAGTGTGTGGAGCCCTTGGGA
GCCTCCAAAATGCTCTATACCTTCCAATGCTCCACCTACCAGATGGGTGT
GCTCCTCATGACGATCTTCAAGTACATCGAGGAGAAGGATGTGTTCATGA
CGTTCTACTCGAAGGCCCTGGCGAAGCGACTCATCCAGGGCCTGAGTGCG
TCGGAGGACCTGGAGGGCAGCATGATCAGCAAACTCAAGTCTGCGTGTGG
GTTCGAGTACACTTCGAAGCTCCAACGGATGTTCACAGACATGACAGTAA
GCAGAGACCTCGAGGAAGCGTTCAGAGAGCGAAACTCTGGAAGTGAAGTG
ACATTGGGCTGTGACTTCAGTGTTCTCGTCCTGGCCACCGGCTCTTGGCC
TCTCACCGCACCCACGACAGGATTTGAGATCCCCAAGGCTCTCGCTGTGT
GTGAGGCAGAGTTCAAGTCATTCTATTGCAAGAAACACTCTGGGAGAAAG
CTCAGTTGGCTCCACCAACACTGCAAGGGAGAGCTGAAGTGTGTGGAGCC
CTTGGGAGCCTCCAAAATGCTCTATACCTTCCAATGCTCCACCTACCAGA
TGGGTGTGCTCCTC
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