mRNA_M-pyrifera_M_contig112069.2527.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig112069.2527.1
Unique NamemRNA_M-pyrifera_M_contig112069.2527.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig112069.2527.1 vs. uniprot
Match: A0A3D5DIC4_9GAMM (Proline--tRNA ligase n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A3D5DIC4_9GAMM)

HSP 1 Score: 201 bits (511), Expect = 2.630e-59
Identity = 97/129 (75.19%), Postives = 112/129 (86.82%), Query Frame = 1 
Query:    1 GCYGIGVSRIVAAAIEQNYDENGIIWPTAIAPFHLAIAPVNAHKSTRVRELAETLYERFATAGIDVLLCDNKERPGVMFANLDLIGIPHRLVLSDRGLDAGTLEYKGRTDDASEDIPLDDALGFIQGKL 387
            GCYGIGVSRIVAAA+EQN+DE GIIWP  IAPF LAIAP+NAHKS+RV+++A +LYE+F TA IDVLL D KERPGVMFANL+LIGIPHRLV+SDRGLD+ TLEYKGRTD+ S +IPL   L FI+ KL
Sbjct:  439 GCYGIGVSRIVAAAVEQNHDERGIIWPAPIAPFDLAIAPINAHKSSRVQKMALSLYEKFKTANIDVLLYDGKERPGVMFANLELIGIPHRLVMSDRGLDSETLEYKGRTDETSNNIPLRSCLDFIKDKL 567
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BLAST of mRNA_M-pyrifera_M_contig112069.2527.1 vs. uniprot
Match: A0A3B1ASP9_9ZZZZ (Proline--tRNA ligase n=1 Tax=hydrothermal vent metagenome TaxID=652676 RepID=A0A3B1ASP9_9ZZZZ)

HSP 1 Score: 187 bits (476), Expect = 3.490e-54
Identity = 86/128 (67.19%), Postives = 105/128 (82.03%), Query Frame = 1 
Query:    1 GCYGIGVSRIVAAAIEQNYDENGIIWPTAIAPFHLAIAPVNAHKSTRVRELAETLYERFATAGIDVLLCDNKERPGVMFANLDLIGIPHRLVLSDRGLDAGTLEYKGRTDDASEDIPLDDALGFIQGK 384
            GCYGIGVSR+VA+AIEQN+D+ GIIWP AIAPFH+AI P+N HKS R+R+  +TLY     AG DVLL D KERPGVMFA+++LIGIPHR+V+ +RGLDAGTLEYKGR+D   +DIPL+ A  F+Q K
Sbjct:  439 GCYGIGVSRVVASAIEQNHDDRGIIWPDAIAPFHVAIVPMNMHKSQRLRDAVDTLYNELTAAGFDVLLDDRKERPGVMFADMELIGIPHRIVIGERGLDAGTLEYKGRSDTDKQDIPLETATRFLQEK 566
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BLAST of mRNA_M-pyrifera_M_contig112069.2527.1 vs. uniprot
Match: A0A7C7QHU5_9GAMM (Proline--tRNA ligase n=1 Tax=Chromatiales bacterium TaxID=2026725 RepID=A0A7C7QHU5_9GAMM)

HSP 1 Score: 176 bits (445), Expect = 2.350e-53
Identity = 83/129 (64.34%), Postives = 101/129 (78.29%), Query Frame = 1 
Query:    1 GCYGIGVSRIVAAAIEQNYDENGIIWPTAIAPFHLAIAPVNAHKSTRVRELAETLYERFATAGIDVLLCDNKERPGVMFANLDLIGIPHRLVLSDRGLDAGTLEYKGRTDDASEDIPLDDALGFIQGKL 387
            GCYGIGVSR+VAAAIEQN+D+ GIIWP AIAPF LA+ P+N HKS R+R+  E LY   A  GI+VLL D KER GVMFA+++LIGIPHR+VL +RGLD G +EYK R D  S+DIPLD  + ++Q KL
Sbjct:   76 GCYGIGVSRVVAAAIEQNHDDKGIIWPEAIAPFQLALLPMNMHKSERLRDAVEALYAELAAVGIEVLLDDRKERAGVMFADMELIGIPHRIVLGERGLDKGMVEYKARRDANSQDIPLDGIVRYLQEKL 204
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BLAST of mRNA_M-pyrifera_M_contig112069.2527.1 vs. uniprot
Match: A0A3M1DL80_9GAMM (Proline--tRNA ligase n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A3M1DL80_9GAMM)

HSP 1 Score: 184 bits (467), Expect = 7.090e-53
Identity = 84/127 (66.14%), Postives = 103/127 (81.10%), Query Frame = 1 
Query:    1 GCYGIGVSRIVAAAIEQNYDENGIIWPTAIAPFHLAIAPVNAHKSTRVRELAETLYERFATAGIDVLLCDNKERPGVMFANLDLIGIPHRLVLSDRGLDAGTLEYKGRTDDASEDIPLDDALGFIQG 381
            GCYGIGVSRIVAAAIEQN+DE GI+WP A+APF LA+ P+N HKS R+RE AE LY     AG++VL  D +ERPGVMF +++LIGIPHRLVLSDRGLDAG+ EY+GR D+AS D+PL+    F++G
Sbjct:  438 GCYGIGVSRIVAAAIEQNHDERGIVWPAAMAPFQLALVPINMHKSQRLREAAEALYRELVEAGVEVLFDDRRERPGVMFNDMELIGIPHRLVLSDRGLDAGSAEYRGRRDEASTDVPLEGLTDFLRG 564
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BLAST of mRNA_M-pyrifera_M_contig112069.2527.1 vs. uniprot
Match: A0A3D5Q3I8_9GAMM (Proline--tRNA ligase (Fragment) n=1 Tax=Marinobacter sp. TaxID=50741 RepID=A0A3D5Q3I8_9GAMM)

HSP 1 Score: 175 bits (443), Expect = 1.480e-52
Identity = 83/125 (66.40%), Postives = 100/125 (80.00%), Query Frame = 1 
Query:    1 GCYGIGVSRIVAAAIEQNYDENGIIWPTAIAPFHLAIAPVNAHKSTRVRELAETLYERFATAGIDVLLCDNKERPGVMFANLDLIGIPHRLVLSDRGLDAGTLEYKGRTDDASEDIPLDDALGFI 375
            GCYGIGVSRIVAAAIEQN+DE GIIWP AIAPF +A+  +NAHKS  VRE  + LYE+   AG DV+L D  ERPGV FA+++L+GIPHR+V+S+RGL AGTLEYKGR D   +DIPL+ AL F+
Sbjct:  110 GCYGIGVSRIVAAAIEQNHDEQGIIWPEAIAPFEVALVTLNAHKSEVVRETGDKLYEQLRQAGFDVILDDRNERPGVKFADMELMGIPHRIVVSERGLKAGTLEYKGRRDADKQDIPLEQALSFL 234
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BLAST of mRNA_M-pyrifera_M_contig112069.2527.1 vs. uniprot
Match: A0A7V2S8W9_9GAMM (Proline--tRNA ligase n=1 Tax=Aeromonadales bacterium TaxID=2268177 RepID=A0A7V2S8W9_9GAMM)

HSP 1 Score: 181 bits (460), Expect = 2.650e-52
Identity = 83/129 (64.34%), Postives = 103/129 (79.84%), Query Frame = 1 
Query:    1 GCYGIGVSRIVAAAIEQNYDENGIIWPTAIAPFHLAIAPVNAHKSTRVRELAETLYERFATAGIDVLLCDNKERPGVMFANLDLIGIPHRLVLSDRGLDAGTLEYKGRTDDASEDIPLDDALGFIQGKL 387
            GCYGIGVSR VAAAIEQN+DE GIIWP AIAPFHLA+ P+N H+S RV++  E LY+   T G++VL  D KERPGVMF +++LIGIPHRLV+ +R +D   +EYKGRTD+ SE+IPLD A+ FI+ KL
Sbjct:  378 GCYGIGVSRTVAAAIEQNHDERGIIWPLAIAPFHLALVPINYHRSERVQQACEQLYQELTTLGVEVLFDDRKERPGVMFNDMELIGIPHRLVIGERAMDNNEIEYKGRTDEVSENIPLDSAVDFIKDKL 506
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BLAST of mRNA_M-pyrifera_M_contig112069.2527.1 vs. uniprot
Match: A7C6A6_9GAMM (Prolyl-tRNA synthetase n=1 Tax=Beggiatoa sp. PS TaxID=422289 RepID=A7C6A6_9GAMM)

HSP 1 Score: 172 bits (435), Expect = 3.920e-52
Identity = 80/129 (62.02%), Postives = 101/129 (78.29%), Query Frame = 1 
Query:    1 GCYGIGVSRIVAAAIEQNYDENGIIWPTAIAPFHLAIAPVNAHKSTRVRELAETLYERFATAGIDVLLCDNKERPGVMFANLDLIGIPHRLVLSDRGLDAGTLEYKGRTDDASEDIPLDDALGFIQGKL 387
            GCYGIGVSR+VAAAIEQN+DE+GIIWP +IAPF + + P++ HKS R+RE AE +Y +  +AGIDVL  D +ER GVMFAN DLIGIPHRLV+ + GLD G +EYKGR D  +  IP+ +A+ FIQ +L
Sbjct:   53 GCYGIGVSRLVAAAIEQNHDEHGIIWPQSIAPFSVILLPMSMHKSQRLREAAEAMYNQLQSAGIDVLFDDRRERAGVMFANADLIGIPHRLVIGESGLDKGEIEYKGRRDQKAHSIPMAEAVSFIQTQL 181
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BLAST of mRNA_M-pyrifera_M_contig112069.2527.1 vs. uniprot
Match: A0A1G0G7M2_9GAMM (Proline--tRNA ligase n=1 Tax=Gammaproteobacteria bacterium RBG_16_51_14 TaxID=1798265 RepID=A0A1G0G7M2_9GAMM)

HSP 1 Score: 181 bits (460), Expect = 7.930e-52
Identity = 87/129 (67.44%), Postives = 103/129 (79.84%), Query Frame = 1 
Query:    1 GCYGIGVSRIVAAAIEQNYDENGIIWPTAIAPFHLAIAPVNAHKSTRVRELAETLYERFATAGIDVLLCDNKERPGVMFANLDLIGIPHRLVLSDRGLDAGTLEYKGRTDDASEDIPLDDALGFIQGKL 387
            GCYGIGVSRIVAAAIEQN+DE GIIWP AIAPF LA+ P+N HKS R+R+     Y     AGI+VLL D KERPGVMFA+ +LIG+PHRLV S+RGLDAGT+EYKGR D  +++IPL DA+ FI+ KL
Sbjct:  439 GCYGIGVSRIVAAAIEQNHDEYGIIWPQAIAPFQLALIPINMHKSQRLRDAVIPFYHDLQNAGIEVLLDDRKERPGVMFADAELIGLPHRLVYSERGLDAGTIEYKGRRDRENQEIPLADAIAFIRSKL 567
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BLAST of mRNA_M-pyrifera_M_contig112069.2527.1 vs. uniprot
Match: UPI00084A01D7 (proline--tRNA ligase n=1 Tax=Candidatus Marithrix sp. Canyon 246 TaxID=1827136 RepID=UPI00084A01D7)

HSP 1 Score: 178 bits (451), Expect = 8.490e-52
Identity = 82/127 (64.57%), Postives = 101/127 (79.53%), Query Frame = 1 
Query:    1 GCYGIGVSRIVAAAIEQNYDENGIIWPTAIAPFHLAIAPVNAHKSTRVRELAETLYERFATAGIDVLLCDNKERPGVMFANLDLIGIPHRLVLSDRGLDAGTLEYKGRTDDASEDIPLDDALGFIQG 381
            GCYGIGVSR+VAA IEQN+D+NGIIWP AIAPF +A+ P+N HKS R+R+ AE LY +   AGIDVL  D KERPGVMFAN +L+GIPHR+V+ DRGLD   +EYKGR D+ S++IP+D  + FI G
Sbjct:  291 GCYGIGVSRLVAATIEQNHDKNGIIWPEAIAPFSVALLPMNMHKSQRLRDAAEDLYNKLQAAGIDVLFDDRKERPGVMFANAELMGIPHRIVIGDRGLDKSEVEYKGRLDEKSQNIPIDKIIEFIVG 417
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BLAST of mRNA_M-pyrifera_M_contig112069.2527.1 vs. uniprot
Match: A0A7C1Y122_9GAMM (Proline--tRNA ligase n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A7C1Y122_9GAMM)

HSP 1 Score: 181 bits (458), Expect = 1.600e-51
Identity = 86/129 (66.67%), Postives = 102/129 (79.07%), Query Frame = 1 
Query:    1 GCYGIGVSRIVAAAIEQNYDENGIIWPTAIAPFHLAIAPVNAHKSTRVRELAETLYERFATAGIDVLLCDNKERPGVMFANLDLIGIPHRLVLSDRGLDAGTLEYKGRTDDASEDIPLDDALGFIQGKL 387
            GCYGIGVSR+VAAAIEQN+D+ GIIWP +IAPF LA+ P+NAHKS RVRE AE LY +F  AGIDVL  D  ER G+MFA+++LIGIPHRLV+ DRGLD G LEYKGR D  + D+PLD  +  I+ KL
Sbjct:  442 GCYGIGVSRVVAAAIEQNHDDRGIIWPQSIAPFQLALLPMNAHKSERVRETAEHLYAKFTAAGIDVLYDDRGERAGIMFADMELIGIPHRLVIGDRGLDKGCLEYKGRRDADNTDVPLDSVVAVIREKL 570
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig112069.2527.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A3D5DIC4_9GAMM2.630e-5975.19Proline--tRNA ligase n=1 Tax=Gammaproteobacteria b... [more]
A0A3B1ASP9_9ZZZZ3.490e-5467.19Proline--tRNA ligase n=1 Tax=hydrothermal vent met... [more]
A0A7C7QHU5_9GAMM2.350e-5364.34Proline--tRNA ligase n=1 Tax=Chromatiales bacteriu... [more]
A0A3M1DL80_9GAMM7.090e-5366.14Proline--tRNA ligase n=1 Tax=Gammaproteobacteria b... [more]
A0A3D5Q3I8_9GAMM1.480e-5266.40Proline--tRNA ligase (Fragment) n=1 Tax=Marinobact... [more]
A0A7V2S8W9_9GAMM2.650e-5264.34Proline--tRNA ligase n=1 Tax=Aeromonadales bacteri... [more]
A7C6A6_9GAMM3.920e-5262.02Prolyl-tRNA synthetase n=1 Tax=Beggiatoa sp. PS Ta... [more]
A0A1G0G7M2_9GAMM7.930e-5267.44Proline--tRNA ligase n=1 Tax=Gammaproteobacteria b... [more]
UPI00084A01D78.490e-5264.57proline--tRNA ligase n=1 Tax=Candidatus Marithrix ... [more]
A0A7C1Y122_9GAMM1.600e-5166.67Proline--tRNA ligase n=1 Tax=Gammaproteobacteria b... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig112069contigM-pyrifera_M_contig112069:175..585 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score182.2
Seed ortholog evalue1.7e-43
Seed eggNOG ortholog626887.J057_23350
Preferred nameproS
KEGG rclassRC00055,RC00523
KEGG koko:K01881
KEGG ReactionR03661
KEGG Pathwayko00970,map00970
KEGG ModuleM00359,M00360
Hectar predicted targeting categoryother localisation
GOsGO:0002161,GO:0003674,GO:0003824,GO:0004812,GO:0004827,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0006082,GO:0006139,GO:0006399,GO:0006412,GO:0006418,GO:0006433,GO:0006450,GO:0006518,GO:0006520,GO:0006725,GO:0006807,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009987,GO:0010467,GO:0016070,GO:0016787,GO:0016788,GO:0016874,GO:0016875,GO:0019538,GO:0019752,GO:0034641,GO:0034645,GO:0034660,GO:0043038,GO:0043039,GO:0043043,GO:0043170,GO:0043436,GO:0043603,GO:0043604,GO:0043906,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044271,GO:0044281,GO:0044424,GO:0044444,GO:0044464,GO:0046483,GO:0052689,GO:0065007,GO:0065008,GO:0071704,GO:0090304,GO:0106074,GO:0140098,GO:0140101,GO:1901360,GO:1901564,GO:1901566,GO:1901576
EggNOG free text desc.Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys- tRNA(Pro) is not edited by ProRS
EggNOG OGs1MU7E@1224,1RN5R@1236,4652J@72275,COG0442@1,COG0442@2
EC6.1.1.15
COG Functional cat.J
BiGG ReactioniJN678.proS,iUTI89_1310.UTI89_C0210
Best tax levelAlteromonadaceae
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko01007,ko03016
Exons1
Model size411
Cds size411
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815634.2758815-CDS-M-pyrifera_M_contig112069:174..5851622815634.2758815-CDS-M-pyrifera_M_contig112069:174..585Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig112069 175..585 -
1692277497.5974965-CDS-M-pyrifera_M_contig112069:174..5851692277497.5974965-CDS-M-pyrifera_M_contig112069:174..585Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig112069 175..585 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig112069.2527.1prot_M-pyrifera_M_contig112069.2527.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig112069 175..585 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig112069.2527.1

>prot_M-pyrifera_M_contig112069.2527.1 ID=prot_M-pyrifera_M_contig112069.2527.1|Name=mRNA_M-pyrifera_M_contig112069.2527.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=137bp
GCYGIGVSRIVAAAIEQNYDENGIIWPTAIAPFHLAIAPVNAHKSTRVRE
LAETLYERFATAGIDVLLCDNKERPGVMFANLDLIGIPHRLVLSDRGLDA
GTLEYKGRTDDASEDIPLDDALGFIQGKLLPSAVTS*
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mRNA from alignment at M-pyrifera_M_contig112069:175..585-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig112069.2527.1 ID=mRNA_M-pyrifera_M_contig112069.2527.1|Name=mRNA_M-pyrifera_M_contig112069.2527.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=411bp|location=Sequence derived from alignment at M-pyrifera_M_contig112069:175..585- (Macrocystis pyrifera P11B4 male)
GGCTGCTATGGCATCGGGGTCTCGCGCATCGTGGCCGCGGCGATCGAGCA
GAATTACGATGAAAACGGCATTATCTGGCCGACCGCGATCGCGCCGTTCC
ATCTTGCCATCGCGCCGGTCAACGCACACAAGTCGACCCGCGTGCGCGAG
CTTGCCGAAACGCTGTACGAGCGCTTCGCGACCGCCGGTATCGATGTGCT
GCTGTGCGACAACAAGGAACGCCCCGGGGTTATGTTCGCAAACCTCGATC
TGATCGGAATTCCGCACCGGCTCGTGCTCAGCGACCGCGGACTCGACGCC
GGCACGCTCGAATACAAGGGCCGTACGGACGACGCGAGCGAGGACATTCC
GCTCGACGACGCGCTTGGGTTTATCCAGGGCAAGCTGTTGCCGAGTGCGG
TGACGTCCTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig112069:175..585-

>mRNA_M-pyrifera_M_contig112069.2527.1 ID=mRNA_M-pyrifera_M_contig112069.2527.1|Name=mRNA_M-pyrifera_M_contig112069.2527.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=822bp|location=Sequence derived from alignment at M-pyrifera_M_contig112069:175..585- (Macrocystis pyrifera P11B4 male)
GGCTGCTATGGCATCGGGGTCTCGCGCATCGTGGCCGCGGCGATCGAGCA
GAATTACGATGAAAACGGCATTATCTGGCCGACCGCGATCGCGCCGTTCC
ATCTTGCCATCGCGCCGGTCAACGCACACAAGTCGACCCGCGTGCGCGAG
CTTGCCGAAACGCTGTACGAGCGCTTCGCGACCGCCGGTATCGATGTGCT
GCTGTGCGACAACAAGGAACGCCCCGGGGTTATGTTCGCAAACCTCGATC
TGATCGGAATTCCGCACCGGCTCGTGCTCAGCGACCGCGGACTCGACGCC
GGCACGCTCGAATACAAGGGCCGTACGGACGACGCGAGCGAGGACATTCC
GCTCGACGACGCGCTTGGGTTTATCCAGGGCAAGCTGTTGCCGAGTGCGG
TGACGTCCTGAGGCTGCTATGGCATCGGGGTCTCGCGCATCGTGGCCGCG
GCGATCGAGCAGAATTACGATGAAAACGGCATTATCTGGCCGACCGCGAT
CGCGCCGTTCCATCTTGCCATCGCGCCGGTCAACGCACACAAGTCGACCC
GCGTGCGCGAGCTTGCCGAAACGCTGTACGAGCGCTTCGCGACCGCCGGT
ATCGATGTGCTGCTGTGCGACAACAAGGAACGCCCCGGGGTTATGTTCGC
AAACCTCGATCTGATCGGAATTCCGCACCGGCTCGTGCTCAGCGACCGCG
GACTCGACGCCGGCACGCTCGAATACAAGGGCCGTACGGACGACGCGAGC
GAGGACATTCCGCTCGACGACGCGCTTGGGTTTATCCAGGGCAAGCTGTT
GCCGAGTGCGGTGACGTCCTGA
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