mRNA_M-pyrifera_M_contig108569.1805.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig108569.1805.1
Unique NamemRNA_M-pyrifera_M_contig108569.1805.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig108569.1805.1 vs. uniprot
Match: A0A7S4HVR9_9EUKA (Hypothetical protein n=2 Tax=Vannella sp. CB-2014 TaxID=1487602 RepID=A0A7S4HVR9_9EUKA)

HSP 1 Score: 90.9 bits (224), Expect = 5.340e-18
Identity = 58/148 (39.19%), Postives = 83/148 (56.08%), Query Frame = 1
Query:    4 GCMVPRVLVALKRRFEVLNGFQTEGIFRVAGNDAECVRIAREIEKGKDAYCERSSNVHNYATLIKRFFAELPHKLL---DKIPSDELIKHKEDFST-LDATLWVDEVHMSVYQWLLTVLLNTLRYSTENKMGINNLAIVWAPVLFDTA 435
            G  VP +LV LK    V++G ++EGIFR AG ++E   +  +I  G+  +   S N H+ ATL+KR+F +LPH+LL   D+  SD  + H E+F T +D TL         + WL  +LL  +    ENKM   N AIVW P +  +A
Sbjct:  188 GIEVPEILVMLKDILIVMDGIESEGIFRKAGLESEMTILREKITNGEPFH---SYNPHSIATLMKRWFKQLPHRLLADADRNLSDAEVIHPENFLTGMDLTL---------FNWLTCLLLLVVEKQEENKMDPKNCAIVWGPGMVGSA 323          
BLAST of mRNA_M-pyrifera_M_contig108569.1805.1 vs. uniprot
Match: A0A7S4I8K4_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Vannella sp. CB-2014 TaxID=1487602 RepID=A0A7S4I8K4_9EUKA)

HSP 1 Score: 86.7 bits (213), Expect = 2.580e-17
Identity = 49/149 (32.89%), Postives = 85/149 (57.05%), Query Frame = 1
Query:    4 GCMVPRVLVALKRRFEVLNGFQTEGIFRVAGNDAECVRIAREIEKGKDAYCERSSNVHNYATLIKRFFAELPHKLLDKIPSDELIKHKEDFSTLDATLWVDEVHMSVYQWLLTVLLNTLRYSTENKMGINNLAIVWAPVLFDTAMSSSD 450
            G  VP +LV L+    +L+ F+ EGIFR AGN+ E  ++ + + KGK  +   S+NVH+ ATL+KR+F ++P ++   I    L +  +     D    +  +  S+ +WL  +L++  ++S  NKM  NNL IV+ P ++ ++  + D
Sbjct:   13 GVDVPEILVILRDMLLLLDPFEVEGIFRKAGNETEMKKMIKALNKGKPIH---STNVHSVATLLKRWFKDIPKRIFAHIHKSLLDEPSQIMRLYDE---LSPMESSLAKWLFELLVDFSKHSDVNKMSPNNLGIVFGPGVYGSSGHAFD 155          
BLAST of mRNA_M-pyrifera_M_contig108569.1805.1 vs. uniprot
Match: A0A7S4HZ80_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Vannella sp. CB-2014 TaxID=1487602 RepID=A0A7S4HZ80_9EUKA)

HSP 1 Score: 84.0 bits (206), Expect = 2.750e-17
Identity = 49/145 (33.79%), Postives = 80/145 (55.17%), Query Frame = 1
Query:    4 GCMVPRVLVALKRRFEVLNGFQTEGIFRVAGNDAECVRIAREIEKGKDAYCERSS----NVHNYATLIKRFFAELP-HKLLDKIPSDELIKHKEDFSTLDATLWVDEVHMSVYQWLLTVLLNTLRYSTENKMGINNLAIVWAPVL 423
            G  VP++L  L+ + +  NG + EGIFR++G++ E + +  E       Y  +++    + HN A L+KR+F      +LL  IP +E  K       +D + ++ E  +S+++WLL +LL+   +   NKM   NLAIVWAP L
Sbjct:   22 GFKVPKILADLRDQMDFTNGLKAEGIFRLSGSETEIIALYEE-------YTAKATVSHFDAHNAANLMKRWFKSWEGSRLLGDIPVEEFQKSPH----IDVSSFLTEPRLSIWKWLLQLLLDVYAFREFNKMSAKNLAIVWAPAL 155          
BLAST of mRNA_M-pyrifera_M_contig108569.1805.1 vs. uniprot
Match: A0A2R5GIT6_9STRA (Rho GTPase activating protein, putative n=1 Tax=Hondaea fermentalgiana TaxID=2315210 RepID=A0A2R5GIT6_9STRA)

HSP 1 Score: 87.4 bits (215), Expect = 8.710e-17
Identity = 54/144 (37.50%), Postives = 80/144 (55.56%), Query Frame = 1
Query:   13 VPRVLVALKRRFEVLNGFQTEGIFRVAGNDAECVRIAREIEKGKDAYC-ERSSNVHNYATLIKRFFAELPHKLLDKIPSDELIKHKE---DFSTLDATLW-VDEVHMSVYQWLLTVLLNTLRYSTENKMGINNLAIVWAPVLFD 429
            +P VLV L+  F+ L GF TEGIFR+A +  EC     ++ KGK A      ++ H  A LIK+FF EL   +L K   D ++   +   D+  ++A++  +DE   SV  WLL ++    +   + KM I NLAIV +P L+D
Sbjct:  352 IPAVLVLLRESFQNLEGFTTEGIFRIAPDGEECSFTKAQLNKGKGADALANCTDPHIPANLIKQFFRELRPNILSKFSRDTVVAIADSAPDYELIEASVMDLDEPGKSVLLWLLDLMCAVSKNKDQTKMTIKNLAIVMSPNLYD 495          
BLAST of mRNA_M-pyrifera_M_contig108569.1805.1 vs. uniprot
Match: A0A2P6NS92_9EUKA (Rac GTPase activating protein n=1 Tax=Planoprotostelium fungivorum TaxID=1890364 RepID=A0A2P6NS92_9EUKA)

HSP 1 Score: 79.7 bits (195), Expect = 4.130e-14
Identity = 48/153 (31.37%), Postives = 84/153 (54.90%), Query Frame = 1
Query:    4 GCMVPRVLVALKRRFEVLNGFQTEGIFRVAGNDAECVRIAREIEKGKDA-YCERSSNVHNYATLIKRFFAELPHKLLDKIPSDEL---IKHKEDFSTLDATLWVDEVHMSVYQWLLTVLLNTLRYSTENKMGINNLAIVWAPVLFDTAMSSSD 450
            G  VP++L  L+R     NG + EG+FR+ G++    RI + I +  D  +     + H+ ATLIKR+F+ LP+KL+  +P + +    + K+D + + A L  +E + ++  WL+ +      YS +N M  NN+A V AP+     + S++
Sbjct:  495 GLHVPKILNQLRRCLITTNGIKCEGLFRITGSETAMKRIVKCIAEYPDEDFPVAGDDYHSIATLIKRWFSNLPYKLMQGLPVEMISPFTEKKKDVTAVAAQL--EEPYGTLLVWLIDLSARVTLYSEKNLMNPNNIARVLAPMSLQITVQSAE 645          
BLAST of mRNA_M-pyrifera_M_contig108569.1805.1 vs. uniprot
Match: A0A2P6NJP7_9EUKA (Rho-GAP domain-containing protein n=1 Tax=Planoprotostelium fungivorum TaxID=1890364 RepID=A0A2P6NJP7_9EUKA)

HSP 1 Score: 76.3 bits (186), Expect = 3.060e-13
Identity = 53/166 (31.93%), Postives = 91/166 (54.82%), Query Frame = 1
Query:    4 GCMVPRVLVALKRRFEVLNGFQTEGIFRVAGNDAECVRIAREI-EKGKDAYCERSSNVHNYATLIKRFFAELPHKLLDKIPSDELIKHKEDFSTLDATLWVDEVHMSVYQWLLTVLLNTLRYSTENKMGINNLAIVWAPVLFDTAMSSSDLARGITVAE--AQRLF 492
            G  VP VL  L+R      G ++EGIFR+AG   E +R+   + +K  DA    +++V+  A+L+K +F +LP  +L+ +P   ++   +    + A   + E   ++  WLL  L+    +S +NKM   N+AIV AP L+D  +S+ +   G+ +++  AQ LF
Sbjct:  143 GRTVPLVLANLRRLLVEKGGQRSEGIFRLAGEQNEILRLKESLNQKRYDAVT--TNDVNAIASLMKIWFRDLPTPILNVLPDTTIMNFHDASDCVQAYHSLPEPQKTLISWLLDFLVEVSSFSAQNKMTAQNIAIVIAPNLYD--ISTPNPMEGLILSQKCAQFLF 304          
BLAST of mRNA_M-pyrifera_M_contig108569.1805.1 vs. uniprot
Match: UPI0002C3D9AB (GTPase activating protein, putative n=1 Tax=Entamoeba invadens IP1 TaxID=370355 RepID=UPI0002C3D9AB)

HSP 1 Score: 73.2 bits (178), Expect = 4.220e-12
Identity = 45/168 (26.79%), Postives = 83/168 (49.40%), Query Frame = 1
Query:   10 MVPRVLVALKRRFEVLNGFQTEGIFRVAGNDAECVRIAREIEKGKDAYCE--RSSNVHNYATLIKRFFAELPHKLLDKIPSDELIKHKEDFSTLDATLWVDEVHMSVYQWLLTVLLNTLRYSTENKMGINNLAIVWAPVLFDTAMSSSDLARGITVAEAQRLFIEDSI 507
            ++P+V+V L   F    GF +EG+FR+AG       +  ++ K   A       + V   + LIK +F ELP  +L+ + SD++    E   +  A   ++E   ++  WL  +++        NKM + NLAIV AP L++    + D   G+ +++    F+++ +
Sbjct:  146 ILPQVIVTLDIAFRERGGFNSEGVFRLAGEQGMVKGLKDKLNKSNGAITNDMMGATVDEISNLIKLWFRELPKPILNVLSSDQIFYSAEAAESYKAYQTLNEKSRTLLDWLFDLMIEVSHNREVNKMTVQNLAIVIAPNLYEP--EAIDPMEGLMMSQKAVQFVQNCL 311          
BLAST of mRNA_M-pyrifera_M_contig108569.1805.1 vs. uniprot
Match: L8HB62_ACACA (RhoGAP domain containing protein n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8HB62_ACACA)

HSP 1 Score: 72.8 bits (177), Expect = 9.420e-12
Identity = 49/169 (28.99%), Postives = 85/169 (50.30%), Query Frame = 1
Query:    4 GCMVPRVLVALKRRFEVLNGFQTEGIFRVAGNDAECVRIAREIEK-------GKDAYCERSSNVHNYATLIKRFFAELPHKLLDKIPSDELIKHKEDFSTLDATLWVDEVH----------MSVYQWLLTVLLNTLRYSTENKMGINNLAIVWAPVLFDTAMSSSDLAR 459
            G  VPR LVAL+R F  +NG   EGIFR+AG++ EC ++   + K       G  ++    ++VH  ++LIKR++ ELP +LL    +          +  D  + ++E+             ++ +L+ +  +  R S  NKM   N+A+V+AP+L +      ++ R
Sbjct:  293 GLTVPRGLVALRRLFYAMNGHMEEGIFRIAGSETECQKMQASLNKPRNVDKQGNFSFDVFPNDVHAVSSLIKRWYRELPVRLLAGSAACVADLSGRLAAADDRAVLLEEIRGFPDSLPPLQKDLFLFLMGLGRDVARNSAVNKMVPRNIAVVFAPMLVERTSPMEEMMR 461          
BLAST of mRNA_M-pyrifera_M_contig108569.1805.1 vs. uniprot
Match: B0EBY3_ENTDS (GTPase activating protein, putative n=6 Tax=Entamoeba TaxID=5758 RepID=B0EBY3_ENTDS)

HSP 1 Score: 71.6 bits (174), Expect = 1.280e-11
Identity = 49/166 (29.52%), Postives = 89/166 (53.61%), Query Frame = 1
Query:   13 VPRVLVALKRRFEVLNGFQTEGIFRVAGNDA--ECVRIAREIEKGKDAYCERSSNVHNYATLIKRFFAELPHKLLDKIPSDEL-IKHKEDFSTLDATLWVDEVHMSVYQWLLTVLLNTLRYSTENKMGINNLAIVWAPVLFDTAMSSSDLARGITVAEAQRLFIED 501
            +P+ L  L   F+ L+GF +EG+FR+AG     + ++      KG D   +    ++  A LIK +F ELP  LL+ + S ++ +   +D      TL   +    +  WL ++L++   +  +NKM + NLAIV AP L+++  +SSD   G+ +++    F+++
Sbjct:  129 LPKPLAILNNNFQRLDGFHSEGVFRLAGQAGLMKTMKSYMNTHKG-DLEVQEEFTINEVANLIKLWFRELPTLLLNGLTSTQIQMSGVDDCWKQYCTL--SQQSRDLLDWLFSLLVHVSLFKDQNKMTLQNLAIVVAPNLYES--TSSDPMEGLMMSQKAVQFVQN 289          
BLAST of mRNA_M-pyrifera_M_contig108569.1805.1 vs. uniprot
Match: A0A0Q3RES8_BRADI (Uncharacterized protein n=1 Tax=Brachypodium distachyon TaxID=15368 RepID=A0A0Q3RES8_BRADI)

HSP 1 Score: 70.5 bits (171), Expect = 3.310e-11
Identity = 50/179 (27.93%), Postives = 88/179 (49.16%), Query Frame = 1
Query:    1 KGCMVPRVLVALKRRFEVLN-GFQTEGIFRVAGNDAECVRIAREIEKGKDAYCERSSN------VHNYATLIKRFFAELPHKLLDKIPSDELIKHKE-DFSTLDATLWVDEVHMSVYQWLLTVLLNTLRYSTENKMGINNLAIVWAPVLFDTAMSSSDLARGITVAEAQRLFIEDSIKK 513
            +G  VP +L+ L+RR      G  TEG+FRVA ++A+   +   +++        SS+      VH  A LIK +F ELP  LLD +P DE+ + +  D +       +     ++  W + ++ +      +N+MG  N+A+V+AP +  T    + L   + V     L IE ++++
Sbjct:   97 RGNSVPTILLLLQRRLYAQGEGLATEGVFRVAADEAQERLVRDHLDRAGAIPSSPSSDNAAAVDVHCLAGLIKAWFRELPGGLLDALPEDEVARCRTADEAARLCAASLPPGKAALLDWAVELMADVAAMEAKNRMGSRNVAMVFAPNMTQTVDPLTALKYAVQVMNFLNLLIERALRQ 275          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig108569.1805.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S4HVR9_9EUKA5.340e-1839.19Hypothetical protein n=2 Tax=Vannella sp. CB-2014 ... [more]
A0A7S4I8K4_9EUKA2.580e-1732.89Hypothetical protein (Fragment) n=1 Tax=Vannella s... [more]
A0A7S4HZ80_9EUKA2.750e-1733.79Hypothetical protein (Fragment) n=1 Tax=Vannella s... [more]
A0A2R5GIT6_9STRA8.710e-1737.50Rho GTPase activating protein, putative n=1 Tax=Ho... [more]
A0A2P6NS92_9EUKA4.130e-1431.37Rac GTPase activating protein n=1 Tax=Planoprotost... [more]
A0A2P6NJP7_9EUKA3.060e-1331.93Rho-GAP domain-containing protein n=1 Tax=Planopro... [more]
UPI0002C3D9AB4.220e-1226.79GTPase activating protein, putative n=1 Tax=Entamo... [more]
L8HB62_ACACA9.420e-1228.99RhoGAP domain containing protein n=1 Tax=Acanthamo... [more]
B0EBY3_ENTDS1.280e-1129.52GTPase activating protein, putative n=6 Tax=Entamo... [more]
A0A0Q3RES8_BRADI3.310e-1127.93Uncharacterized protein n=1 Tax=Brachypodium dista... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig108569contigM-pyrifera_M_contig108569:2..544 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score87.4
Seed ortholog evalue7.2e-15
Seed eggNOG ortholog5786.XP_003285287.1
KEGG koko:K20642
Hectar predicted targeting categoryother localisation
EggNOG free text desc.GTPase-activator protein for Rho-like GTPases
EggNOG OGs3X8IK@554915,KOG4270@1,KOG4270@2759
COG Functional cat.T
Best tax levelAmoebozoa
Best eggNOG OGNA|NA|NA
BRITEko00000,ko04131
Exons1
Model size543
Cds size534
Stop0
Start1
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815595.601792-UTR-M-pyrifera_M_contig108569:1..101622815595.601792-UTR-M-pyrifera_M_contig108569:1..10Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig108569 2..10 +
1692277479.6388414-UTR-M-pyrifera_M_contig108569:1..101692277479.6388414-UTR-M-pyrifera_M_contig108569:1..10Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig108569 2..10 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815595.6178672-CDS-M-pyrifera_M_contig108569:10..5441622815595.6178672-CDS-M-pyrifera_M_contig108569:10..544Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig108569 11..544 +
1692277479.6506531-CDS-M-pyrifera_M_contig108569:10..5441692277479.6506531-CDS-M-pyrifera_M_contig108569:10..544Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig108569 11..544 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig108569.1805.1prot_M-pyrifera_M_contig108569.1805.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig108569 11..544 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig108569.1805.1

>prot_M-pyrifera_M_contig108569.1805.1 ID=prot_M-pyrifera_M_contig108569.1805.1|Name=mRNA_M-pyrifera_M_contig108569.1805.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=178bp
MVPRVLVALKRRFEVLNGFQTEGIFRVAGNDAECVRIAREIEKGKDAYCE
RSSNVHNYATLIKRFFAELPHKLLDKIPSDELIKHKEDFSTLDATLWVDE
VHMSVYQWLLTVLLNTLRYSTENKMGINNLAIVWAPVLFDTAMSSSDLAR
GITVAEAQRLFIEDSIKKELGLSEEERA
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mRNA from alignment at M-pyrifera_M_contig108569:2..544+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig108569.1805.1 ID=mRNA_M-pyrifera_M_contig108569.1805.1|Name=mRNA_M-pyrifera_M_contig108569.1805.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=543bp|location=Sequence derived from alignment at M-pyrifera_M_contig108569:2..544+ (Macrocystis pyrifera P11B4 male)
AAAGGGTGTATGGTCCCTCGTGTGCTGGTGGCGTTGAAGAGGAGATTCGA GGTGCTCAACGGATTCCAAACAGAAGGGATCTTCCGCGTTGCTGGCAATG ACGCAGAGTGCGTGAGGATTGCGCGCGAGATAGAGAAAGGGAAGGATGCC TACTGTGAGCGCTCTTCCAACGTTCACAACTACGCCACTCTAATCAAACG GTTCTTCGCAGAGCTCCCTCATAAGCTCTTGGACAAGATCCCTTCCGACG AGCTCATCAAGCACAAGGAGGACTTTTCCACCCTCGACGCCACTCTGTGG GTCGACGAGGTGCACATGAGTGTCTACCAGTGGCTGCTTACAGTGCTGCT GAATACCCTTCGCTACTCTACAGAGAACAAGATGGGAATCAACAACCTGG CAATCGTTTGGGCGCCGGTGCTCTTCGACACCGCAATGTCGAGCTCCGAC CTCGCCAGGGGCATCACCGTAGCAGAGGCCCAGCGCCTCTTCATCGAAGA CTCGATCAAGAAGGAGCTGGGTCTTTCTGAAGAGGAGAGGGCA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig108569:2..544+

>mRNA_M-pyrifera_M_contig108569.1805.1 ID=mRNA_M-pyrifera_M_contig108569.1805.1|Name=mRNA_M-pyrifera_M_contig108569.1805.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1068bp|location=Sequence derived from alignment at M-pyrifera_M_contig108569:2..544+ (Macrocystis pyrifera P11B4 male)
ATGGTCCCTCGTGTGCTGGTGGCGTTGAAGAGGAGATTCGAGGTGCTCAA
CGGATTCCAAACAGAAGGGATCTTCCGCGTTGCTGGCAATGACGCAGAGT
GCGTGAGGATTGCGCGCGAGATAGAGAAAGGGAAGGATGCCTACTGTGAG
CGCTCTTCCAACGTTCACAACTACGCCACTCTAATCAAACGGTTCTTCGC
AGAGCTCCCTCATAAGCTCTTGGACAAGATCCCTTCCGACGAGCTCATCA
AGCACAAGGAGGACTTTTCCACCCTCGACGCCACTCTGTGGGTCGACGAG
GTGCACATGAGTGTCTACCAGTGGCTGCTTACAGTGCTGCTGAATACCCT
TCGCTACTCTACAGAGAACAAGATGGGAATCAACAACCTGGCAATCGTTT
GGGCGCCGGTGCTCTTCGACACCGCAATGTCGAGCTCCGACCTCGCCAGG
GGCATCACCGTAGCAGAGGCCCAGCGCCTCTTCATCGAAGACTCGATCAA
GAAGGAGCTGGGTCTTTCTGAAGAGGAGAGGGCAATGGTCCCTCGTGTGC
TGGTGGCGTTGAAGAGGAGATTCGAGGTGCTCAACGGATTCCAAACAGAA
GGGATCTTCCGCGTTGCTGGCAATGACGCAGAGTGCGTGAGGATTGCGCG
CGAGATAGAGAAAGGGAAGGATGCCTACTGTGAGCGCTCTTCCAACGTTC
ACAACTACGCCACTCTAATCAAACGGTTCTTCGCAGAGCTCCCTCATAAG
CTCTTGGACAAGATCCCTTCCGACGAGCTCATCAAGCACAAGGAGGACTT
TTCCACCCTCGACGCCACTCTGTGGGTCGACGAGGTGCACATGAGTGTCT
ACCAGTGGCTGCTTACAGTGCTGCTGAATACCCTTCGCTACTCTACAGAG
AACAAGATGGGAATCAACAACCTGGCAATCGTTTGGGCGCCGGTGCTCTT
CGACACCGCAATGTCGAGCTCCGACCTCGCCAGGGGCATCACCGTAGCAG
AGGCCCAGCGCCTCTTCATCGAAGACTCGATCAAGAAGGAGCTGGGTCTT
TCTGAAGAGGAGAGGGCA
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