mRNA_M-pyrifera_M_contig108152.1733.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig108152.1733.1
Unique NamemRNA_M-pyrifera_M_contig108152.1733.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig108152.1733.1 vs. uniprot
Match: A0A151ZJR5_9MYCE (Protein RFT1 homolog n=1 Tax=Tieghemostelium lacteum TaxID=361077 RepID=A0A151ZJR5_9MYCE)

HSP 1 Score: 110 bits (274), Expect = 2.820e-25
Identity = 67/164 (40.85%), Postives = 96/164 (58.54%), Query Frame = 1
Query:   10 GLMAFAYGQLAQSFVVLGGYVWYFWRRIGKQGGSAEDRLPFTSFGELLPSIGALPVQ-----VVTLVATFSWQSVEKLLLTEGEKFVLKFTETLVHQGSFSVVSSLGSLVARFLFQPVEEVSFSLFSKLLGDVRSKEGHSRAASSLAMCDDILAMLVKFMFLIG 486
            GL  F Y QL+ S V++ GY  YF     + G +       TS  +LLP    L  +      + +   ++WQS++KL+LTEGEKFVL  +E+L++Q  FSVVS+LGSL ARFLFQP+EE  FS+F KL       +  S+   ++     ILA+++KFM +I 
Sbjct:  193 GLKGFGYSQLSYSLVLVIGYYGYFIIDTIRNGNNKNQENLITSLSQLLPKKTVLSHRWFDSSAIRMTMLYTWQSIQKLVLTEGEKFVLYLSESLLNQAIFSVVSALGSLAARFLFQPIEESCFSMFPKLYKATTDSD-KSKDKENVK----ILALIMKFMIIIA 351          
BLAST of mRNA_M-pyrifera_M_contig108152.1733.1 vs. uniprot
Match: D3BUS7_POLPP (Protein RFT1 homolog n=1 Tax=Polysphondylium pallidum (strain ATCC 26659 / Pp 5 / PN500) TaxID=670386 RepID=D3BUS7_POLPP)

HSP 1 Score: 108 bits (271), Expect = 3.240e-25
Identity = 65/164 (39.63%), Postives = 92/164 (56.10%), Query Frame = 1
Query:   10 GLMAFAYGQLAQSFVVLGGYVWYFWRRIGKQGGSAEDR-LPFTSFGELLP----SIGALPVQVVTLVATFSWQSVEKLLLTEGEKFVLKFTETLVHQGSFSVVSSLGSLVARFLFQPVEEVSFSLFSKLLGDVRSKEGHSRAASSLAMCDDILAMLVKFMFLIG 486
            GL  F Y Q+  S  ++ GY   F   I +     +D+ +   S  +LLP    +   +   +  L   ++WQS++KLLLTEGEKFVL F+E L  Q  FSVVS+LGSL+ARF FQP+EE  FS+F KL G+   ++  S  +        +L ML+K M +I 
Sbjct:   32 GLKGFGYAQIIYSLTLVIGYYGNFIITIYQDTNKQDDKSIQIRSLNQLLPKPIKNDSFISADLYKLTGLYTWQSIQKLLLTEGEKFVLYFSENLTSQAIFSVVSNLGSLIARFFFQPIEESCFSMFPKLFGESTRRQDWSDGSK-------VLTMLMKLMIIIA 188          
BLAST of mRNA_M-pyrifera_M_contig108152.1733.1 vs. uniprot
Match: A0A6B2L231_9EUKA (Protein RFT1 homolog (Fragment) n=1 Tax=Arcella intermedia TaxID=1963864 RepID=A0A6B2L231_9EUKA)

HSP 1 Score: 107 bits (267), Expect = 3.180e-24
Identity = 65/166 (39.16%), Postives = 102/166 (61.45%), Query Frame = 1
Query:    4 QVGLMAFAYGQLAQSFVVLGGYVWYFWRRIGKQGGSAEDRLPFTSFGELLPS-IGALP----VQVVTLVATFSWQSVEKLLLTEGEKFVLKFTETLVHQGSFSVVSSLGSLVARFLFQPVEEVSFSLFSKLLGDVRSKEGHSRAASSLAMCDDILAMLVKFMFLIG 486
            Q+ L++F  GQ+  S +++ GY  YF+ +I K+    +    FT   +L P+ +G++        + L+  F WQS +KL+L EGEK VL F E+L +QG +SVV++LGSLV RFLFQP+EEV +++FSKL    +S E   + +S   +   +L++ +K  F IG
Sbjct:  150 QLDLLSFGIGQIVYSLILIVGYFGYFFYKIMKRNTETQ----FTHIRQLFPTYLGSMKESFDTSSIKLLINFEWQSCQKLVLQEGEKIVLTFAESLFNQGIYSVVNNLGSLVVRFLFQPLEEVIYAVFSKL----KSYEKKDKNSSQETL--RVLSITIKLSFYIG 305          
BLAST of mRNA_M-pyrifera_M_contig108152.1733.1 vs. uniprot
Match: A0A8J4Q114_9MYCE (Protein RFT1 homolog n=1 Tax=Polysphondylium violaceum TaxID=133409 RepID=A0A8J4Q114_9MYCE)

HSP 1 Score: 106 bits (264), Expect = 1.080e-23
Identity = 62/173 (35.84%), Postives = 95/173 (54.91%), Query Frame = 1
Query:   10 GLMAFAYGQLAQSFVVLGGYVWYFWRRI--------GKQGGSAEDRLPFTSFGELLPSIG-ALPVQVVTLVATFSWQSVEKLLLTEGEKFVLKFTETLVHQGSFSVVSSLGSLVARFLFQPVEEVSFSLFSKLLGDVRSKEGHS-----RAASSLAMCDDILAMLVKFMFLIG 486
            GL  F Y QL  S  +  GY  YF  ++                ++  F SF ++LP+   ++   ++ L   ++WQ+++KLLLTEGEK++L F+ETLV Q  FSVVS+LGSL+ RFLF P+EE  F +F KL   + +   ++          L     ILA+++KF+ +I 
Sbjct:  197 GLKGFGYSQLFYSLSIFLGYFGYFIYKVLNTXXXXXXXXXXXXXEQFQFNSFKQILPNPSKSIDKHLLKLTGLYTWQTIQKLLLTEGEKYILYFSETLVSQAIFSVVSNLGSLIVRFLFLPIEESCFLMFPKLFPSISNSSNNNIIDKKEKEKELESGSKILAVVIKFLMIIA 369          
BLAST of mRNA_M-pyrifera_M_contig108152.1733.1 vs. uniprot
Match: UPI00064508B8 (hypothetical protein n=1 Tax=Acytostelium subglobosum LB1 TaxID=1410327 RepID=UPI00064508B8)

HSP 1 Score: 103 bits (257), Expect = 9.890e-23
Identity = 68/177 (38.42%), Postives = 96/177 (54.24%), Query Frame = 1
Query:   10 GLMAFAYGQLAQSFVVLGGYVWYFWRRI-----GKQGGSAEDRLPFTSFGELLP---SIGA------------LPVQVVTLVATFSWQSVEKLLLTEGEKFVLKFTETLVHQGSFSVVSSLGSLVARFLFQPVEEVSFSLFSKLLGDVRSKEGHSRAASSLAMCDDILAMLVKFMFL 480
            GL  F Y Q+  S V+L GY   F   I         G     +  TSF +LLP   SIG                 +V +   ++WQS++KLLLTEGEK VL  +++LV Q  FSVVS+LGSLVARF FQP+EE  F++F KL G    K+  + ++  L +   +++ +V F+F+
Sbjct:  204 GLKGFGYAQIIYSVVLLIGYYGNFIIGILQYDRSNMSGKFTSGVQITSFNQLLPRRPSIGTGTSKDKERDRDIFGKDLVDMTMLYTWQSIQKLLLTEGEKIVLYLSQSLVSQAIFSVVSNLGSLVARFFFQPIEETCFTMFPKLFGAKSRKDDWADSSKVLTLLMKVMS-IVAFIFI 379          
BLAST of mRNA_M-pyrifera_M_contig108152.1733.1 vs. uniprot
Match: F4PY40_CAVFA (Protein RFT1 homolog n=1 Tax=Cavenderia fasciculata (strain SH3) TaxID=1054147 RepID=F4PY40_CAVFA)

HSP 1 Score: 101 bits (251), Expect = 7.100e-22
Identity = 66/169 (39.05%), Postives = 94/169 (55.62%), Query Frame = 1
Query:    7 VGLMAFAYGQLAQSFVVLGGYVWYFWRRIGK----QGGSAEDRLPFTSFGELLPSIGALPVQVVT----LVATFSWQSVEKLLLTEGEKFVLKFTET-LVHQGSFSVVSSLGSLVARFLFQPVEEVSFSLFSKLLGDVRSKEGHSRAASSLAMCDDILAMLVKFMFLIG 486
            +GL  F Y Q+  S V++ GY   F   I +    +GG    ++  TS  +LLP    + V V +    + A ++WQS++KLLLTEGEK V+      LV QG FSVVS+LGSLVARF  QP+EE  F++F KL  + +  +   R          +L +L+K M +IG
Sbjct:  217 MGLKGFGYAQVVYSVVLIVGYYGNFILSIIRSNRGKGGDTAGQVTITSVSQLLPRFSGINVLVGSELWYMTAMYTWQSIQKLLLTEGEKMVMYTNSVDLVSQGIFSVVSNLGSLVARFFLQPIEESCFAMFPKLFANRQDWDTGHR----------VLTLLMKMMIIIG 375          
BLAST of mRNA_M-pyrifera_M_contig108152.1733.1 vs. uniprot
Match: UPI001401DB87 (protein RFT1 homolog isoform X1 n=3 Tax=Amblyraja radiata TaxID=386614 RepID=UPI001401DB87)

HSP 1 Score: 94.7 bits (234), Expect = 1.240e-19
Identity = 61/165 (36.97%), Postives = 92/165 (55.76%), Query Frame = 1
Query:   10 GLMAFAYGQLAQSFVVLGGYVWYFWRRIGKQGGSAEDRLPFTSFGELLPSI----GALPVQVVTLVATFSWQSVEKLLLTEGEKFVLKFTETLV--HQGSFSVVSSLGSLVARFLFQPVEEVSFSLFSKLLGDVRSKEGHSRAASSLAMCDDILAMLVKFMFLIG 486
            GL  F+  Q+  SF +L  Y+ YF + IG +    +   P TS  +LLP        + +Q+  L  TF  QS+ K +LTEGE++V+ F   L    QG + +V++LGSLVARFLF P+EE  +  F+ +L   R K+   +    + +   +L ML+K +FLIG
Sbjct:  178 GLYVFSLAQILHSFTLLACYLLYFMKFIGSREAQRKS-FPLTSLTDLLPKPLPRQHVIDLQLAKLTWTFYKQSMLKQILTEGERYVMTFLNVLNFGEQGIYDIVNNLGSLVARFLFLPIEESFYIFFANVLE--RGKDPRHQREEDVGLAALVLKMLLKMVFLIG 339          
BLAST of mRNA_M-pyrifera_M_contig108152.1733.1 vs. uniprot
Match: UPI001E1D3BE3 (protein RFT1 homolog n=1 Tax=Mercenaria mercenaria TaxID=6596 RepID=UPI001E1D3BE3)

HSP 1 Score: 93.2 bits (230), Expect = 4.510e-19
Identity = 64/167 (38.32%), Postives = 90/167 (53.89%), Query Frame = 1
Query:    4 QVGLMAFAYGQLAQSFVVLGGYVWYFWRRIGKQGGSAEDRLPFTSFGELLPS----IGALPVQVVTLVATFSWQSVEKLLLTEGEKFVLKFTETL--VHQGSFSVVSSLGSLVARFLFQPVEEVSFSLFSKLLGDVRSKEGHSRAASSLAMCDDILAMLVKFMFLIG 486
            Q+GL+ FA  Q+    V+ G Y  YF   I K G   +D  PF+S  +LLPS       + V    L  +   QS  K LLTEGEK+V+     L    QG + +V++LGS+ ARF+FQP+EE  +  FS+LL  VR +    +   S  +  D+L  L+K + LIG
Sbjct:  220 QLGLINFAIAQMVYGVVMSGVYYAYFIFYINKYG-KKDDSFPFSSVKQLLPSKQKDSSFVDVHQAWLTFSIFKQSFLKQLLTEGEKYVMTIFGVLSFADQGIYDIVNNLGSMAARFIFQPIEESGYLFFSQLL--VRGEPVSKQKQESCQLAVDVLGYLLKLVILIG 383          
BLAST of mRNA_M-pyrifera_M_contig108152.1733.1 vs. uniprot
Match: RFT1_DICDI (Protein RFT1 homolog n=1 Tax=Dictyostelium discoideum TaxID=44689 RepID=RFT1_DICDI)

HSP 1 Score: 92.0 bits (227), Expect = 1.100e-18
Identity = 54/129 (41.86%), Postives = 74/129 (57.36%), Query Frame = 1
Query:    7 VGLMAFAYGQLAQSFVVLGGYVWYFWRRI---GKQGGSAEDRLPFTSFGELLPSIGA-LPVQVVTLVATFSWQSVEKLLLTEGEKFVLKFTETLVHQGSFSVVSSLGSLVARFLFQPVEEVSFSLFSKL 381
            +GL+ F Y Q+  S  ++ GY  YF   I    K   + E    F S  +L P     +   ++ L   ++WQS+ KLLL EGEKFVL F+ET   Q  F++VS+LGSL+ RFLF P+EE  F +F KL
Sbjct:  185 MGLIGFGYAQILYSLTLVIGYFGYFLINIINNNKNKDNKEFSNCFKSIDQLFPKFSTRIDRNLIKLSLLYTWQSIYKLLLQEGEKFVLFFSETNQGQAIFAIVSNLGSLIVRFLFLPIEETCFLMFPKL 313          
BLAST of mRNA_M-pyrifera_M_contig108152.1733.1 vs. uniprot
Match: UPI0018F74847 (protein RFT1 homolog n=1 Tax=Scyliorhinus canicula TaxID=7830 RepID=UPI0018F74847)

HSP 1 Score: 90.5 bits (223), Expect = 3.720e-18
Identity = 62/165 (37.58%), Postives = 90/165 (54.55%), Query Frame = 1
Query:   10 GLMAFAYGQLAQSFVVLGGYVWYFWRRIGKQGGSAEDRLPFTSFGELLPSI----GALPVQVVTLVATFSWQSVEKLLLTEGEKFVLKFTETLVH--QGSFSVVSSLGSLVARFLFQPVEEVSFSLFSKLLGDVRSKEGHSRAASSLAMCDDILAMLVKFMFLIG 486
            GL  F+  QL  S  +L  YV YF + +G Q    +   P ++ G+L+P        L  ++  L  TF  QS  K +LTEGE++V+ F   L    QG + +V++LGSLVARFLF P+EE  +  F K+L   R K+   +    ++M   +L MLVK + LIG
Sbjct:  164 GLCIFSLAQLLYSSCLLACYVLYFVKFVGSQEAQRKS-FPISTLGDLMPKFLCHQHVLNPRLAILTWTFYKQSFLKQVLTEGERYVMTFLNVLNFGDQGVYDIVNNLGSLVARFLFLPIEESFYVFFVKVLE--RGKDIGHQKREDVSMAATVLEMLVKLVLLIG 325          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig108152.1733.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A151ZJR5_9MYCE2.820e-2540.85Protein RFT1 homolog n=1 Tax=Tieghemostelium lacte... [more]
D3BUS7_POLPP3.240e-2539.63Protein RFT1 homolog n=1 Tax=Polysphondylium palli... [more]
A0A6B2L231_9EUKA3.180e-2439.16Protein RFT1 homolog (Fragment) n=1 Tax=Arcella in... [more]
A0A8J4Q114_9MYCE1.080e-2335.84Protein RFT1 homolog n=1 Tax=Polysphondylium viola... [more]
UPI00064508B89.890e-2338.42hypothetical protein n=1 Tax=Acytostelium subglobo... [more]
F4PY40_CAVFA7.100e-2239.05Protein RFT1 homolog n=1 Tax=Cavenderia fasciculat... [more]
UPI001401DB871.240e-1936.97protein RFT1 homolog isoform X1 n=3 Tax=Amblyraja ... [more]
UPI001E1D3BE34.510e-1938.32protein RFT1 homolog n=1 Tax=Mercenaria mercenaria... [more]
RFT1_DICDI1.100e-1841.86Protein RFT1 homolog n=1 Tax=Dictyostelium discoid... [more]
UPI0018F748473.720e-1837.58protein RFT1 homolog n=1 Tax=Scyliorhinus canicula... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig108152contigM-pyrifera_M_contig108152:13..547 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score87.0
Seed ortholog evalue8.3e-15
Seed eggNOG ortholog44689.DDB0305027
Preferred nameRFT1
KEGG koko:K02738,ko:K06316,ko:K11369
KEGG TC2.A.66.3
KEGG Pathwayko03050,map03050
KEGG ModuleM00340
Hectar predicted targeting categoryother localisation
GOsGO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005783,GO:0005789,GO:0006810,GO:0006869,GO:0008150,GO:0009987,GO:0010876,GO:0012505,GO:0016020,GO:0016021,GO:0016043,GO:0031224,GO:0031984,GO:0033036,GO:0034203,GO:0034204,GO:0042175,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044422,GO:0044424,GO:0044425,GO:0044432,GO:0044444,GO:0044446,GO:0044464,GO:0046836,GO:0051179,GO:0051234,GO:0061024,GO:0065007,GO:0065008,GO:0071702,GO:0071840,GO:0097035,GO:0098827,GO:1901264
EggNOG free text desc.Rft protein
EggNOG OGs3X8V6@554915,KOG2864@1,KOG2864@2759
EC3.4.25.1
COG Functional cat.U
Best tax levelAmoebozoa
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko01002,ko02000,ko03036,ko03051
Exons2
Model size486
Cds size471
Stop0
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig108152.1733.1prot_M-pyrifera_M_contig108152.1733.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig108152 28..547 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815591.3787994-UTR-M-pyrifera_M_contig108152:12..271622815591.3787994-UTR-M-pyrifera_M_contig108152:12..27Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig108152 13..27 +
1692277477.9426246-UTR-M-pyrifera_M_contig108152:12..271692277477.9426246-UTR-M-pyrifera_M_contig108152:12..27Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig108152 13..27 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815591.3892403-CDS-M-pyrifera_M_contig108152:27..3251622815591.3892403-CDS-M-pyrifera_M_contig108152:27..325Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig108152 28..325 +
1692277477.9552493-CDS-M-pyrifera_M_contig108152:27..3251692277477.9552493-CDS-M-pyrifera_M_contig108152:27..325Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig108152 28..325 +
1622815591.4041407-CDS-M-pyrifera_M_contig108152:374..5471622815591.4041407-CDS-M-pyrifera_M_contig108152:374..547Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig108152 375..547 +
1692277477.9642289-CDS-M-pyrifera_M_contig108152:374..5471692277477.9642289-CDS-M-pyrifera_M_contig108152:374..547Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig108152 375..547 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig108152.1733.1

>prot_M-pyrifera_M_contig108152.1733.1 ID=prot_M-pyrifera_M_contig108152.1733.1|Name=mRNA_M-pyrifera_M_contig108152.1733.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=157bp
MAFAYGQLAQSFVVLGGYVWYFWRRIGKQGGSAEDRLPFTSFGELLPSIG
ALPVQVVTLVATFSWQSVEKLLLTEGEKFVLKFTETLVHQGSFSVVSSLG
SLVARFLFQPVEEVSFSLFSKLLGDVRSKEGHSRAASSLAMCDDILAMLV
KFMFLIG
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mRNA from alignment at M-pyrifera_M_contig108152:13..547+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig108152.1733.1 ID=mRNA_M-pyrifera_M_contig108152.1733.1|Name=mRNA_M-pyrifera_M_contig108152.1733.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=535bp|location=Sequence derived from alignment at M-pyrifera_M_contig108152:13..547+ (Macrocystis pyrifera P11B4 male)
GTGCAGGTGGGCTTGATGGCTTTTGCCTATGGACAGCTTGCGCAATCTTT CGTGGTGCTTGGCGGGTATGTGTGGTACTTTTGGAGAAGAATAGGCAAAC AAGGGGGCTCAGCTGAGGATAGGCTCCCGTTCACGAGCTTTGGGGAGCTA CTGCCCTCCATTGGTGCCCTGCCAGTGCAAGTGGTCACCCTGGTGGCCAC CTTTTCTTGGCAGTCTGTGGAGAAACTGCTCTTGACTGAGGGGGAAAAGT TTGTTCTGAAGTTCACAGAGACACTAGTTCATCAAGGAAGCTTCTCAGTC GTAAGCAGCCTTGGTAGGTGCCTCTGTCACGTCAGCCACCCGCTATGACT GACAAACCGCAGGCTCGTTGGTGGCGCGCTTCCTCTTTCAGCCAGTGGAA GAGGTGAGCTTCAGCCTCTTCAGTAAGCTGCTGGGAGACGTGAGGTCCAA GGAGGGCCACAGCAGAGCGGCCTCCAGCCTCGCCATGTGCGACGACATTC TCGCGATGCTCGTCAAGTTCATGTTTCTCATTGGT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig108152:13..547+

>mRNA_M-pyrifera_M_contig108152.1733.1 ID=mRNA_M-pyrifera_M_contig108152.1733.1|Name=mRNA_M-pyrifera_M_contig108152.1733.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=942bp|location=Sequence derived from alignment at M-pyrifera_M_contig108152:13..547+ (Macrocystis pyrifera P11B4 male)
ATGGCTTTTGCCTATGGACAGCTTGCGCAATCTTTCGTGGTGCTTGGCGG
GTATGTGTGGTACTTTTGGAGAAGAATAGGCAAACAAGGGGGCTCAGCTG
AGGATAGGCTCCCGTTCACGAGCTTTGGGGAGCTACTGCCCTCCATTGGT
GCCCTGCCAGTGCAAGTGGTCACCCTGGTGGCCACCTTTTCTTGGCAGTC
TGTGGAGAAACTGCTCTTGACTGAGGGGGAAAAGTTTGTTCTGAAGTTCA
CAGAGACACTAGTTCATCAAGGAAGCTTCTCAGTCGTAAGCAGCCTTGAT
GGCTTTTGCCTATGGACAGCTTGCGCAATCTTTCGTGGTGCTTGGCGGGT
ATGTGTGGTACTTTTGGAGAAGAATAGGCAAACAAGGGGGCTCAGCTGAG
GATAGGCTCCCGTTCACGAGCTTTGGGGAGCTACTGCCCTCCATTGGTGC
CCTGCCAGTGCAAGTGGTCACCCTGGTGGCCACCTTTTCTTGGCAGTCTG
TGGAGAAACTGCTCTTGACTGAGGGGGAAAAGTTTGTTCTGAAGTTCACA
GAGACACTAGTTCATCAAGGAAGCTTCTCAGTCGTAAGCAGCCTTGGCTC
GTTGGTGGCGCGCTTCCTCTTTCAGCCAGTGGAAGAGGTGAGCTTCAGCC
TCTTCAGTAAGCTGCTGGGAGACGTGAGGTCCAAGGAGGGCCACAGCAGA
GCGGCCTCCAGCCTCGCCATGTGCGACGACATTCTCGCGATGCTCGTCAA
GTTCATGTTTCTCATTGGTGCTCGTTGGTGGCGCGCTTCCTCTTTCAGCC
AGTGGAAGAGGTGAGCTTCAGCCTCTTCAGTAAGCTGCTGGGAGACGTGA
GGTCCAAGGAGGGCCACAGCAGAGCGGCCTCCAGCCTCGCCATGTGCGAC
GACATTCTCGCGATGCTCGTCAAGTTCATGTTTCTCATTGGT
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