mRNA_M-pyrifera_M_contig107971.1696.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig107971.1696.1
Unique NamemRNA_M-pyrifera_M_contig107971.1696.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig107971.1696.1 vs. uniprot
Match: D8LJ58_ECTSI (ER membrane protein complex subunit 2 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LJ58_ECTSI)

HSP 1 Score: 92.8 bits (229), Expect = 3.490e-21
Identity = 48/72 (66.67%), Postives = 55/72 (76.39%), Query Frame = 1
Query:    1 MATAAMDAGDLAAADKQLGVLQLKFPGSQRVKRLQGMRSEAGGDFKAAGKIYEEMVAQNPANSLARKRQVCI 216
            M TAA+D+GD+  A + L VL  KFPGSQRV+RL+GMR EA G F AA  IYEEMV  NPANSLARKRQV +
Sbjct:   64 MVTAALDSGDVPTAHEHLQVLTRKFPGSQRVRRLEGMRCEAEGSFAAAAAIYEEMVEANPANSLARKRQVAV 135          
BLAST of mRNA_M-pyrifera_M_contig107971.1696.1 vs. uniprot
Match: A0A6H5KPY1_9PHAE (ER membrane protein complex subunit 2 n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KPY1_9PHAE)

HSP 1 Score: 92.8 bits (229), Expect = 3.620e-21
Identity = 48/72 (66.67%), Postives = 55/72 (76.39%), Query Frame = 1
Query:    1 MATAAMDAGDLAAADKQLGVLQLKFPGSQRVKRLQGMRSEAGGDFKAAGKIYEEMVAQNPANSLARKRQVCI 216
            M TAA+D+GD+  A + L VL  KFPGSQRV+RL+GMR EA G F AA  IYEEMV  NPANSLARKRQV +
Sbjct:   64 MVTAALDSGDVPTAHEHLQVLTRKFPGSQRVRRLEGMRCEAEGSFAAAAAIYEEMVEANPANSLARKRQVAV 135          
BLAST of mRNA_M-pyrifera_M_contig107971.1696.1 vs. uniprot
Match: A0A7S2GPJ3_9STRA (ER membrane protein complex subunit 2 n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2GPJ3_9STRA)

HSP 1 Score: 73.6 bits (179), Expect = 8.860e-14
Identity = 38/69 (55.07%), Postives = 52/69 (75.36%), Query Frame = 1
Query:   10 AAMDAGDLAAADKQLGVLQLKFPGSQRVKRLQGMRSEAGGDFKAAGKIYEEMVAQNPANSLARKRQVCI 216
            AA+D  D   + + L  LQ KFP S RVKRL+GM+SEA G++ AA +IY++M+ +NPAN+LA KRQVC+
Sbjct:   59 AALDLHDTELSKECLKELQTKFPESARVKRLEGMQSEALGEWGAAKEIYKQMLEENPANTLAMKRQVCV 127          
BLAST of mRNA_M-pyrifera_M_contig107971.1696.1 vs. uniprot
Match: A0A197JL96_9FUNG (ER membrane protein complex subunit 2 n=1 Tax=Linnemannia elongata AG-77 TaxID=1314771 RepID=A0A197JL96_9FUNG)

HSP 1 Score: 62.8 bits (151), Expect = 8.430e-10
Identity = 34/72 (47.22%), Postives = 48/72 (66.67%), Query Frame = 1
Query:    1 MATAAMDAGDLAAADKQLGVLQLKFPGSQRVKRLQGMRSEAGGDFKAAGKIYEEMVAQNPANSLARKRQVCI 216
            +A AA+D GD   A K +  L+ +FPGS RV+RLQGM+ EA G    AG+IY+ ++ ++  N LA KRQV +
Sbjct:   49 VAIAALDMGDDGLALKCIQALETRFPGSPRVRRLQGMQLEAVGKLAEAGQIYKAILEEDETNVLASKRQVML 120          
BLAST of mRNA_M-pyrifera_M_contig107971.1696.1 vs. uniprot
Match: A0A7S3XW17_HETAK (ER membrane protein complex subunit 2 (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XW17_HETAK)

HSP 1 Score: 60.8 bits (146), Expect = 2.380e-9
Identity = 30/69 (43.48%), Postives = 45/69 (65.22%), Query Frame = 1
Query:   10 AAMDAGDLAAADKQLGVLQLKFPGSQRVKRLQGMRSEAGGDFKAAGKIYEEMVAQNPANSLARKRQVCI 216
            A++D G+   A+  L  L+ KFPGS RVKRL G+  E+  ++  A  +Y E++A NPAN+LA KR+  +
Sbjct:   62 ASLDLGEQEVANHALNALKKKFPGSSRVKRLAGLYEESEENYPQAEALYAELLAANPANTLALKRRAAV 130          
BLAST of mRNA_M-pyrifera_M_contig107971.1696.1 vs. uniprot
Match: A0A7S4EW27_CHRCT (ER membrane protein complex subunit 2 n=1 Tax=Chrysotila carterae TaxID=13221 RepID=A0A7S4EW27_CHRCT)

HSP 1 Score: 60.1 bits (144), Expect = 9.540e-9
Identity = 31/61 (50.82%), Postives = 43/61 (70.49%), Query Frame = 1
Query:   34 AAADKQLGVLQLKFPGSQRVKRLQGMRSEAGGDFKAAGKIYEEMVAQNPANSLARKRQVCI 216
            A A++ LG L  +FP S RVKRL+ M SEAGG+ +AA + YE+++ Q+P N  A KRQV +
Sbjct:   97 ALAEQYLGRLSTQFPESLRVKRLEAMMSEAGGELEAAMQKYEDILKQDPNNLYAMKRQVAL 157          
BLAST of mRNA_M-pyrifera_M_contig107971.1696.1 vs. uniprot
Match: A0A8J2WTM1_9STRA (ER membrane protein complex subunit 2 n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2WTM1_9STRA)

HSP 1 Score: 58.9 bits (141), Expect = 2.620e-8
Identity = 33/73 (45.21%), Postives = 48/73 (65.75%), Query Frame = 1
Query:   10 AAMDAGDLAAADKQLGVLQLKF----PGSQRVKRLQGMRSEAGGDFKAAGKIYEEMVAQNPANSLARKRQVCI 216
            AA+D  D    D     L  +F    P SQR++RL+G+ +EA GD++AA + Y+ ++AQNPANS A KR+VC+
Sbjct:   71 AALDVDDAPLRDACQSALLKRFGGANPPSQRLERLEGLVAEAAGDWEAALQRYKGVLAQNPANSTALKREVCV 143          
BLAST of mRNA_M-pyrifera_M_contig107971.1696.1 vs. uniprot
Match: UPI001F03A39B (LOW QUALITY PROTEIN: ER membrane protein complex subunit 2-like n=1 Tax=Xenia sp. Carnegie-2017 TaxID=2897299 RepID=UPI001F03A39B)

HSP 1 Score: 58.2 bits (139), Expect = 4.230e-8
Identity = 31/69 (44.93%), Postives = 42/69 (60.87%), Query Frame = 1
Query:   10 AAMDAGDLAAADKQLGVLQLKFPGSQRVKRLQGMRSEAGGDFKAAGKIYEEMVAQNPANSLARKRQVCI 216
            AA+D G    A   L  L  +FPGS RVKRL  M+ EA   F  A +IY +M+  +PANS+A KR++ +
Sbjct:   58 AALDLGKTDTASMCLKALNAQFPGSLRVKRLIAMKHEAMNQFDKATEIYNDMIKNDPANSIAYKRKISL 126          
BLAST of mRNA_M-pyrifera_M_contig107971.1696.1 vs. uniprot
Match: M5FY72_DACPD (ER membrane protein complex subunit 2 n=1 Tax=Dacryopinax primogenitus (strain DJM 731) TaxID=1858805 RepID=M5FY72_DACPD)

HSP 1 Score: 57.8 bits (138), Expect = 5.710e-8
Identity = 29/70 (41.43%), Postives = 44/70 (62.86%), Query Frame = 1
Query:   10 AAMDAGDLAAADKQLGVLQLKFPGSQRVKRLQGMRSEAGGDFKAAGKIYEEMVAQNPANSLARKRQVCIH 219
            AA+D GD A A +    L  +FPGSQRV+ LQG+R EA G+   A ++YEE++    +N +  KR + ++
Sbjct:   49 AALDMGDTALAGELFSQLDQRFPGSQRVEMLQGVRLEADGELDTARRLYEEILQGEESNLIIWKRLITVY 118          
BLAST of mRNA_M-pyrifera_M_contig107971.1696.1 vs. uniprot
Match: A0A3P8YY18_ESOLU (ER membrane protein complex subunit 2 n=1 Tax=Esox lucius TaxID=8010 RepID=A0A3P8YY18_ESOLU)

HSP 1 Score: 57.4 bits (137), Expect = 7.660e-8
Identity = 27/52 (51.92%), Postives = 39/52 (75.00%), Query Frame = 1
Query:   61 LQLKFPGSQRVKRLQGMRSEAGGDFKAAGKIYEEMVAQNPANSLARKRQVCI 216
            L+ +FPGSQRVKRL GMR EA   ++ AGK Y+ ++ ++P N+ ARKR++ I
Sbjct:   93 LKKQFPGSQRVKRLGGMRLEALEKYEEAGKQYDSILQEDPTNTAARKRKISI 144          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig107971.1696.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LJ58_ECTSI3.490e-2166.67ER membrane protein complex subunit 2 n=1 Tax=Ecto... [more]
A0A6H5KPY1_9PHAE3.620e-2166.67ER membrane protein complex subunit 2 n=1 Tax=Ecto... [more]
A0A7S2GPJ3_9STRA8.860e-1455.07ER membrane protein complex subunit 2 n=1 Tax=Dict... [more]
A0A197JL96_9FUNG8.430e-1047.22ER membrane protein complex subunit 2 n=1 Tax=Linn... [more]
A0A7S3XW17_HETAK2.380e-943.48ER membrane protein complex subunit 2 (Fragment) n... [more]
A0A7S4EW27_CHRCT9.540e-950.82ER membrane protein complex subunit 2 n=1 Tax=Chry... [more]
A0A8J2WTM1_9STRA2.620e-845.21ER membrane protein complex subunit 2 n=1 Tax=Pela... [more]
UPI001F03A39B4.230e-844.93LOW QUALITY PROTEIN: ER membrane protein complex s... [more]
M5FY72_DACPD5.710e-841.43ER membrane protein complex subunit 2 n=1 Tax=Dacr... [more]
A0A3P8YY18_ESOLU7.660e-851.92ER membrane protein complex subunit 2 n=1 Tax=Esox... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig107971contigM-pyrifera_M_contig107971:93..338 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score92.8
Seed ortholog evalue7.9e-17
Seed eggNOG ortholog2880.D8LJ58
Preferred nameEMC2
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005783,GO:0005789,GO:0006457,GO:0008150,GO:0009987,GO:0012505,GO:0016020,GO:0016021,GO:0016043,GO:0022406,GO:0030176,GO:0031224,GO:0031227,GO:0031984,GO:0032991,GO:0034975,GO:0042175,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044422,GO:0044424,GO:0044425,GO:0044432,GO:0044444,GO:0044446,GO:0044464,GO:0051179,GO:0051640,GO:0051641,GO:0071840,GO:0072546,GO:0098796,GO:0098827,GO:0140056,GO:1990456
EggNOG free text desc.protein folding in endoplasmic reticulum
EggNOG OGsKOG3060@1,KOG3060@2759
Ec32 ortholog descriptionTetratricopeptide-like helical domain
Ec32 orthologEc-02_004860.1
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Exons1
Model size246
Cds size246
Stop1
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815589.4648697-CDS-M-pyrifera_M_contig107971:92..3381622815589.4648697-CDS-M-pyrifera_M_contig107971:92..338Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig107971 93..338 +
1692277477.0893946-CDS-M-pyrifera_M_contig107971:92..3381692277477.0893946-CDS-M-pyrifera_M_contig107971:92..338Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig107971 93..338 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig107971.1696.1prot_M-pyrifera_M_contig107971.1696.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig107971 93..338 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig107971.1696.1

>prot_M-pyrifera_M_contig107971.1696.1 ID=prot_M-pyrifera_M_contig107971.1696.1|Name=mRNA_M-pyrifera_M_contig107971.1696.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=82bp
MATAAMDAGDLAAADKQLGVLQLKFPGSQRVKRLQGMRSEAGGDFKAAGK
IYEEMVAQNPANSLARKRQVCIHAFVRARFL*
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mRNA from alignment at M-pyrifera_M_contig107971:93..338+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig107971.1696.1 ID=mRNA_M-pyrifera_M_contig107971.1696.1|Name=mRNA_M-pyrifera_M_contig107971.1696.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=246bp|location=Sequence derived from alignment at M-pyrifera_M_contig107971:93..338+ (Macrocystis pyrifera P11B4 male)
ATGGCGACGGCGGCAATGGACGCGGGCGATCTCGCGGCCGCAGACAAGCA GCTGGGCGTCCTCCAGCTCAAGTTTCCTGGAAGTCAGAGGGTGAAACGGC TGCAAGGCATGCGAAGCGAAGCCGGGGGAGACTTCAAGGCGGCGGGCAAG ATTTACGAAGAGATGGTGGCGCAGAACCCGGCTAACTCGCTCGCGCGCAA GCGACAGGTATGTATACATGCGTTCGTTCGTGCGAGGTTTTTGTAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig107971:93..338+

>mRNA_M-pyrifera_M_contig107971.1696.1 ID=mRNA_M-pyrifera_M_contig107971.1696.1|Name=mRNA_M-pyrifera_M_contig107971.1696.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=492bp|location=Sequence derived from alignment at M-pyrifera_M_contig107971:93..338+ (Macrocystis pyrifera P11B4 male)
ATGGCGACGGCGGCAATGGACGCGGGCGATCTCGCGGCCGCAGACAAGCA
GCTGGGCGTCCTCCAGCTCAAGTTTCCTGGAAGTCAGAGGGTGAAACGGC
TGCAAGGCATGCGAAGCGAAGCCGGGGGAGACTTCAAGGCGGCGGGCAAG
ATTTACGAAGAGATGGTGGCGCAGAACCCGGCTAACTCGCTCGCGCGCAA
GCGACAGGTATGTATACATGCGTTCGTTCGTGCGAGGTTTTTGTAGATGG
CGACGGCGGCAATGGACGCGGGCGATCTCGCGGCCGCAGACAAGCAGCTG
GGCGTCCTCCAGCTCAAGTTTCCTGGAAGTCAGAGGGTGAAACGGCTGCA
AGGCATGCGAAGCGAAGCCGGGGGAGACTTCAAGGCGGCGGGCAAGATTT
ACGAAGAGATGGTGGCGCAGAACCCGGCTAACTCGCTCGCGCGCAAGCGA
CAGGTATGTATACATGCGTTCGTTCGTGCGAGGTTTTTGTAG
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