mRNA_M-pyrifera_M_contig107527.1610.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig107527.1610.1
Unique NamemRNA_M-pyrifera_M_contig107527.1610.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig107527.1610.1 vs. uniprot
Match: A0A2D6QAM7_9ARCH (VWFA domain-containing protein n=3 Tax=cellular organisms TaxID=131567 RepID=A0A2D6QAM7_9ARCH)

HSP 1 Score: 90.9 bits (224), Expect = 9.590e-18
Identity = 79/199 (39.70%), Postives = 103/199 (51.76%), Query Frame = 1
Query:    7 RRVILLVDGSGSMEGAPFDAVKRATLELVQAAPAQDEVALAFFNDRLGASWVLRPAQTGPLFRQAWTPERQADELRQMLDSNVPGGDTRILQTLKGLIGLRKRTVAHPAVVILLSDGAENALDSGDPLELLAVLDEAKALADELRGLDTQLSVISIQGGARTAEEDWAARQLLSALVAPGDELIEV-DLS--AGAAERA 594
            R V+LLVDGSGSM G PF+ V+ A  ELV AA   D + L FFN+ LG        QT         P  +  EL  +L + VP G T IL  L  L   R+RT A   +V+LLSDG  +A+ +    E  A L  ++        LD  LSV+++         + A R  L +L+APG+ELIE  DLS  AG  +RA
Sbjct:  433 RDVVLLVDGSGSMAGEPFERVREALFELVPAALPVDRLELRFFNESLGPVEFRSTGQT---------PAERRKELSPLLHAAVPHGGTDILYALGQLAESRRRTDAS-GLVLLLSDGRSSAVSASAGAEARAALTRSR--------LD--LSVLAVG--------EHADRGCLPSLLAPGEELIEAGDLSSLAGLLQRA 603          
BLAST of mRNA_M-pyrifera_M_contig107527.1610.1 vs. uniprot
Match: A0A7C1WWI6_9BACT (VWA domain-containing protein n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A7C1WWI6_9BACT)

HSP 1 Score: 87.0 bits (214), Expect = 2.080e-16
Identity = 74/182 (40.66%), Postives = 94/182 (51.65%), Query Frame = 1
Query:    7 RRVILLVDGSGSMEGAPFDAVKRATLELVQAAPAQDEVALAFFNDRLGASWVLRPAQTGPLFRQAWTPERQADELRQMLDSNVPGGDTRILQTLKGLIGLRKRTVAHP-AVVILLSDGAENALDSGDPLELLAVLDEAKALADELRGLDTQLSVISIQGGARTAEEDWAARQLLSALVAPGD 549
            R V+LLVDGSGSM+GAPF+ V+ A L+LV AA   D V L FFN  L     ++P   G     A   E      R ++D  VP GDT IL +L+ L   R R  A   A+V+LL+DG    LD GDP +    +D    L  +L    T+L  ++I   A  A  D  A    SAL  PGD
Sbjct:  435 REVLLLVDGSGSMDGAPFEMVRSAALDLVDAALPTDRVVLRFFNVGLREPHEVKPWTDGA----AQDREAAVAAARALVDLTVPSGDTWILSSLEELA--RSRAGAREEALVLLLTDG----LDRGDPTDRDERID---TLLRDLAAAKTRLVPVAIGADADMAFLDRLAGPGASAL-RPGD 602          
BLAST of mRNA_M-pyrifera_M_contig107527.1610.1 vs. uniprot
Match: A0A800KL23_9BACT (VWA domain-containing protein n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A800KL23_9BACT)

HSP 1 Score: 86.3 bits (212), Expect = 3.810e-16
Identity = 75/191 (39.27%), Postives = 98/191 (51.31%), Query Frame = 1
Query:    4 PRRVILLVDGSGSMEGAPFDAVKRATLELVQAAPAQDEVALAFFNDRLGASWVLRPAQTGPLFRQAWTPERQADELRQMLDSNVPGGDTRILQTLKGLIGLRKRTVAHPAVVILLSDGAENALDSGDPLELLAVLDEAKALADELRGLDTQLSVISIQGGARTAEEDWAARQLLSALVAPGDELIEV-DLS 573
            PR V+LLVDGSGSM G PF+ V+ A  ELV AA   D + L FFN+ LG        QT         P  +  EL  +L + VP G T IL  L  L   R+RT A P +V+LLSDG   A+ +    ++ A L  A+        LD  LSV+++         + A R  L +L+  G+ELIE  DLS
Sbjct:  432 PRDVVLLVDGSGSMAGEPFERVREALFELVPAALPVDRLELRFFNESLGPVEFRSTGQT---------PAERRRELSPLLGAAVPHGGTDILYALGQLSKSRERTDA-PGLVLLLSDGRSTAVSAEAGAQVRAGLMRAR--------LD--LSVLAVG--------EHADRGCLPSLLIRGEELIEAGDLS 594          
BLAST of mRNA_M-pyrifera_M_contig107527.1610.1 vs. uniprot
Match: A0A7C7YVC4_9BACT (VWA domain-containing protein n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A7C7YVC4_9BACT)

HSP 1 Score: 83.2 bits (204), Expect = 4.420e-15
Identity = 64/152 (42.11%), Postives = 85/152 (55.92%), Query Frame = 1
Query:    1 PPRRVILLVDGSGSMEGAPFDAVKRATLELVQAAPAQDEVALAFFNDRLGASWVLRPAQTGPLFRQAWTPERQADELRQMLDSNVPGGDTRILQTLKGLIGLRKRTVAHPAVVILLSDGAENALDSGDPLELLAVLDEAKALA-DELRGLDT 453
            PPR V+LL+DGSGSM G PF+ V+RA  ELV+  P  D + L FF+  LG    L    TGP  R        A  LR +    +PGG T IL  L+ L  +R+R+   PAVV++L+DG ++  ++ D LE  A +  A A    ELR L T
Sbjct:  423 PPRDVLLLIDGSGSMAGEPFEEVRRAASELVRCLPEGDSLGLRFFSAGLGGRVDLG---TGPAAR--------AKTLRTLAGLQLPGGATAILYALEELQRVRQRSEG-PAVVLMLTDGRDD--NAFDVLERGAAIRAAFAAGGSELRLLAT 560          
BLAST of mRNA_M-pyrifera_M_contig107527.1610.1 vs. uniprot
Match: A0A2D5W7L3_9BACT (VWFA domain-containing protein n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A2D5W7L3_9BACT)

HSP 1 Score: 82.8 bits (203), Expect = 6.140e-15
Identity = 69/187 (36.90%), Postives = 95/187 (50.80%), Query Frame = 1
Query:    7 RRVILLVDGSGSMEGAPFDAVKRATLELVQAAPAQDEVALAFFNDRLGASWVLRPAQTGPLFRQAWTPERQADELRQMLDSNVPGGDTRILQTLKGLIGLRKRTVAH-PAVVILLSDGAENALDSGDPLELLAVLDEAKALADELRGLDTQLSVISIQGGARTAEEDWAARQLLSALVAPGDELIEV 564
            R VILLVDGSGSM G PF+ V+R  LEL +A+ A D ++L FF   LG      P Q   +   A T  R+A  L  +L + VPGG T +L +L  L+  R R      A+ +LL+DG   + D+          +  + ++ +L     QL V  I         + A RQ L  LVAPG+ L+EV
Sbjct:  472 RDVILLVDGSGSMAGGPFELVRRGVLELARASLAGDHLSLRFFTGVLG------PQQNEAVLPGAGT--RRAT-LLGILAARVPGGTTDVLYSLDQLVAERGRQAERRAALALLLTDGHTASSDA----------ERTREVSRKLVAAGVQLRVFPIG--------EQADRQFLGQLVAPGESLVEV 631          
BLAST of mRNA_M-pyrifera_M_contig107527.1610.1 vs. uniprot
Match: A0A523PVL8_9BACT (VWA domain-containing protein n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A523PVL8_9BACT)

HSP 1 Score: 77.4 bits (189), Expect = 4.370e-13
Identity = 63/193 (32.64%), Postives = 97/193 (50.26%), Query Frame = 1
Query:    4 PRRVILLVDGSGSMEGAPFDAVKRATLELVQAAPAQDEVALAFFNDRLGASWVLRPAQTGPLFRQA--WTPERQADELRQMLDSNVPGGDTRILQTLKGLIGLRKRTVAHPAVVILLSDGAENALDSGDPLELLAVLDEAKALADELRGLDTQLSVISIQGGARTAEEDWAARQLLSALVAPGDELIEV-DLS 573
            PR V+L+VDGSGSM G PF+ V+ A+L+LV  A  +D + L FF   LG            +F  A   + E++   L  +L + VPGG T IL  L  L  LR+     P +V+LL+DG               V + ++AL   L    T+L+++++         +   R+ L++L+  G+ L+E  DLS
Sbjct:  412 PRDVVLIVDGSGSMTGGPFEHVRTASLDLVLGALPRDHIELRFFTGALGPK----------IFESASGQSVEQRQLALAPLLAARVPGGPTDILYMLGQLATLRE-AATRPGLVLLLTDGQTGGW----------VPERSRALRRRLAVAGTKLTILAVG--------EQPDREFLASLLLAGEALVEAGDLS 575          
BLAST of mRNA_M-pyrifera_M_contig107527.1610.1 vs. uniprot
Match: A0A2E5X676_9BACT (VWFA domain-containing protein (Fragment) n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A2E5X676_9BACT)

HSP 1 Score: 73.9 bits (180), Expect = 6.670e-12
Identity = 67/196 (34.18%), Postives = 91/196 (46.43%), Query Frame = 1
Query:    7 RRVILLVDGSGSMEGAPFDAVKRATLELVQAAPAQDEVALAFFN----------DRLGASWVLRPAQTGPLFRQAWTPERQADELRQMLDSNVPGGDTRILQTLKGLIGLRKRTVAHPAVVILLSDGAENALDSGDPLELLAVLDEAKALADELRGLDTQLSVISIQGGARTAEEDWAARQLLSALVAPGDELIEV 564
            R VIL+VDGSGSM G PF+ V+R  LEL +A+ A D +AL FF           DR G +     A TG          R+ + L  +L + VPGG T +L +L  L   R       A+V+LL+DG     ++          + A+A+A  L      L V  I   A         R+ L  LVA G+ L+EV
Sbjct:  530 RDVILVVDGSGSMAGEPFEEVRRGVLELARASLAGDHLALRFFTGVLGPDETGADRSGTAGAHIGAPTGARAGAG----RRRETLLGILAARVPGGTTDVLYSLDQLAEERSGRERR-ALVLLLTDGRTALWEA----------ERARAVAGRLAAAGVHLRVFPIGADAD--------REFLGQLVAEGEGLVEV 702          
BLAST of mRNA_M-pyrifera_M_contig107527.1610.1 vs. uniprot
Match: A0A2D5AST5_9BACT (VWFA domain-containing protein n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A2D5AST5_9BACT)

HSP 1 Score: 71.6 bits (174), Expect = 4.270e-11
Identity = 66/196 (33.67%), Postives = 99/196 (50.51%), Query Frame = 1
Query:    1 PPRRVILLVDGSGSMEGAPFDAVKRATLELVQAAPAQDEVALAFFNDRLGASWVL--RPAQTGPLFRQAWTPERQADEL----RQMLDSNVPGGDTRILQTLKGLIGLRKRTVAHPAVVILLSDGAENALDSGDPLELLAVLD-EAKALADELRGLDTQLSVISIQGGARTAEEDWAARQLLSALVAPGDELIEVD 567
            PPR V+LLVDGSGSM G PF+ V+ ATL+LV AA   D V+L FF   LG + ++  R   TG    ++ T +R A E     R++++  VP G T +L +L+     +        + +LL+DG +         E +A       AL   LR    ++ V++I     +AEE   A   L  LV  G+E++  +
Sbjct:  414 PPREVVLLVDGSGSMAGEPFELVRGATLDLVAAALPSDRVSLRFFRTGLGPARLIKSRTESTGGESGRSQT-DRNALEAQRAARRLIELEVPEGSTFVLSSLEHFAEAKYE---DEVLALLLTDGEDR--------EGIANFSARTAALQARLRATRKRVVVVAI-----SAEE--RALSFLRDLVPDGEEVLRAE 590          
BLAST of mRNA_M-pyrifera_M_contig107527.1610.1 vs. uniprot
Match: A0A2D6QD22_9ARCH (VWFA domain-containing protein n=2 Tax=cellular organisms TaxID=131567 RepID=A0A2D6QD22_9ARCH)

HSP 1 Score: 70.1 bits (170), Expect = 1.440e-10
Identity = 60/177 (33.90%), Postives = 88/177 (49.72%), Query Frame = 1
Query:    4 PRRVILLVDGSGSMEGAPFDAVKRATLELVQAAPAQDEVALAFFNDRLGASWVLRPAQTGPLFRQAWTPERQAD---ELRQMLDSNVPGGDTRILQTLKGLIGLRKRTVAHPAVVILLSDGAENALDSGDPLELLAVLDEAKALADELRGLDTQLSVISIQGGARTAEEDWAARQLL 525
            PR V+L+VDGSGSM G P+  V+RA + L Q+  + D + L  F  RL            PL   A  P R++D    L  +L +++PGG T +   L  L+  R    +  ++ IL+SDG  +    G P         +  L D+L      L+VI+++GG R A E  A R+LL
Sbjct:  414 PRDVVLVVDGSGSMVGLPWQRVQRAVVRLAQSLLSSDGLVLHVFTTRL----------MEPLLSVA--PGRESDLGKALDDLLSAHLPGGATDVAYALTELVKQRAEA-SRRSLCILISDGHSS--QGGHP---------SLDLRDDLGDARADLAVIAVEGGLRRANE-VALRRLL 565          
BLAST of mRNA_M-pyrifera_M_contig107527.1610.1 vs. uniprot
Match: A0A518BJL4_9BACT (von Willebrand factor type A domain protein n=1 Tax=Planctomycetes bacterium Pla133 TaxID=2528011 RepID=A0A518BJL4_9BACT)

HSP 1 Score: 68.6 bits (166), Expect = 4.860e-10
Identity = 44/121 (36.36%), Postives = 66/121 (54.55%), Query Frame = 1
Query:    1 PPRRVILLVDGSGSMEGAPFDAVKRATLELVQAAPAQDEVALAFFNDRLGASWVLRPAQTGPLFRQAWTPERQADELRQMLDSNVPGGDTRILQTLKGLIGLRKRTVAHPAVVILLSDGAE 363
            PPR V+LLVDGSGSM G PF+ V+RA ++L  AAPA D + L FF   L  + VL   ++G           +A    +++ +    G T +L +++ L   R+       +V+LL+DG E
Sbjct:  404 PPRDVLLLVDGSGSMAGEPFEQVERAAVDLAGAAPADDRLLLRFFTSVLQPNVVLED-ESG---------RGRAAVAERLVGARAASGPTALLASIEQLAAERRVDHERAMLVLLLTDGRE 514          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig107527.1610.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 12
Match NameE-valueIdentityDescription
A0A2D6QAM7_9ARCH9.590e-1839.70VWFA domain-containing protein n=3 Tax=cellular or... [more]
A0A7C1WWI6_9BACT2.080e-1640.66VWA domain-containing protein n=1 Tax=Planctomycet... [more]
A0A800KL23_9BACT3.810e-1639.27VWA domain-containing protein n=1 Tax=Planctomycet... [more]
A0A7C7YVC4_9BACT4.420e-1542.11VWA domain-containing protein n=1 Tax=Planctomycet... [more]
A0A2D5W7L3_9BACT6.140e-1536.90VWFA domain-containing protein n=1 Tax=Planctomyce... [more]
A0A523PVL8_9BACT4.370e-1332.64VWA domain-containing protein n=1 Tax=Planctomycet... [more]
A0A2E5X676_9BACT6.670e-1234.18VWFA domain-containing protein (Fragment) n=1 Tax=... [more]
A0A2D5AST5_9BACT4.270e-1133.67VWFA domain-containing protein n=1 Tax=Planctomyce... [more]
A0A2D6QD22_9ARCH1.440e-1033.90VWFA domain-containing protein n=2 Tax=cellular or... [more]
A0A518BJL4_9BACT4.860e-1036.36von Willebrand factor type A domain protein n=1 Ta... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig107527contigM-pyrifera_M_contig107527:12..605 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Hectar predicted targeting categoryother localisation
Exons1
Model size594
Cds size594
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815585.3978553-CDS-M-pyrifera_M_contig107527:11..6051622815585.3978553-CDS-M-pyrifera_M_contig107527:11..605Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig107527 12..605 -
1692277475.4494638-CDS-M-pyrifera_M_contig107527:11..6051692277475.4494638-CDS-M-pyrifera_M_contig107527:11..605Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig107527 12..605 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig107527.1610.1prot_M-pyrifera_M_contig107527.1610.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig107527 12..605 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig107527.1610.1

>prot_M-pyrifera_M_contig107527.1610.1 ID=prot_M-pyrifera_M_contig107527.1610.1|Name=mRNA_M-pyrifera_M_contig107527.1610.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=198bp
PPRRVILLVDGSGSMEGAPFDAVKRATLELVQAAPAQDEVALAFFNDRLG
ASWVLRPAQTGPLFRQAWTPERQADELRQMLDSNVPGGDTRILQTLKGLI
GLRKRTVAHPAVVILLSDGAENALDSGDPLELLAVLDEAKALADELRGLD
TQLSVISIQGGARTAEEDWAARQLLSALVAPGDELIEVDLSAGAAERA
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mRNA from alignment at M-pyrifera_M_contig107527:12..605-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig107527.1610.1 ID=mRNA_M-pyrifera_M_contig107527.1610.1|Name=mRNA_M-pyrifera_M_contig107527.1610.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=594bp|location=Sequence derived from alignment at M-pyrifera_M_contig107527:12..605- (Macrocystis pyrifera P11B4 male)
CCGCCGCGGCGCGTGATCCTGTTGGTCGACGGCTCGGGGTCGATGGAGGG CGCGCCGTTCGACGCGGTCAAGCGCGCGACGCTGGAGCTCGTCCAGGCCG CGCCGGCCCAGGACGAGGTCGCGCTGGCCTTCTTCAACGACCGCCTGGGC GCGTCGTGGGTCTTGCGCCCGGCCCAGACCGGGCCGCTCTTTCGCCAGGC GTGGACCCCCGAGCGCCAGGCCGACGAGCTGCGCCAGATGCTGGACAGCA ACGTGCCCGGCGGCGACACGCGCATCCTGCAGACGTTGAAGGGCTTGATC GGCCTGCGCAAGCGCACGGTCGCGCACCCCGCGGTCGTGATCCTGCTGTC GGACGGCGCGGAGAACGCGCTCGACTCGGGCGACCCGCTGGAGCTCTTGG CGGTGTTGGACGAGGCCAAGGCGCTGGCCGACGAGCTGCGCGGGCTGGAC ACGCAGCTCAGCGTGATCTCGATCCAGGGCGGCGCGCGGACGGCGGAGGA GGACTGGGCGGCGCGGCAGCTGCTCTCGGCGCTGGTCGCGCCTGGGGACG AGCTGATCGAGGTCGACCTGAGCGCCGGGGCGGCGGAGCGGGCG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig107527:12..605-

>mRNA_M-pyrifera_M_contig107527.1610.1 ID=mRNA_M-pyrifera_M_contig107527.1610.1|Name=mRNA_M-pyrifera_M_contig107527.1610.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1188bp|location=Sequence derived from alignment at M-pyrifera_M_contig107527:12..605- (Macrocystis pyrifera P11B4 male)
CCGCCGCGGCGCGTGATCCTGTTGGTCGACGGCTCGGGGTCGATGGAGGG
CGCGCCGTTCGACGCGGTCAAGCGCGCGACGCTGGAGCTCGTCCAGGCCG
CGCCGGCCCAGGACGAGGTCGCGCTGGCCTTCTTCAACGACCGCCTGGGC
GCGTCGTGGGTCTTGCGCCCGGCCCAGACCGGGCCGCTCTTTCGCCAGGC
GTGGACCCCCGAGCGCCAGGCCGACGAGCTGCGCCAGATGCTGGACAGCA
ACGTGCCCGGCGGCGACACGCGCATCCTGCAGACGTTGAAGGGCTTGATC
GGCCTGCGCAAGCGCACGGTCGCGCACCCCGCGGTCGTGATCCTGCTGTC
GGACGGCGCGGAGAACGCGCTCGACTCGGGCGACCCGCTGGAGCTCTTGG
CGGTGTTGGACGAGGCCAAGGCGCTGGCCGACGAGCTGCGCGGGCTGGAC
ACGCAGCTCAGCGTGATCTCGATCCAGGGCGGCGCGCGGACGGCGGAGGA
GGACTGGGCGGCGCGGCAGCTGCTCTCGGCGCTGGTCGCGCCTGGGGACG
AGCTGATCGAGGTCGACCTGAGCGCCGGGGCGGCGGAGCGGGCGCCGCCG
CGGCGCGTGATCCTGTTGGTCGACGGCTCGGGGTCGATGGAGGGCGCGCC
GTTCGACGCGGTCAAGCGCGCGACGCTGGAGCTCGTCCAGGCCGCGCCGG
CCCAGGACGAGGTCGCGCTGGCCTTCTTCAACGACCGCCTGGGCGCGTCG
TGGGTCTTGCGCCCGGCCCAGACCGGGCCGCTCTTTCGCCAGGCGTGGAC
CCCCGAGCGCCAGGCCGACGAGCTGCGCCAGATGCTGGACAGCAACGTGC
CCGGCGGCGACACGCGCATCCTGCAGACGTTGAAGGGCTTGATCGGCCTG
CGCAAGCGCACGGTCGCGCACCCCGCGGTCGTGATCCTGCTGTCGGACGG
CGCGGAGAACGCGCTCGACTCGGGCGACCCGCTGGAGCTCTTGGCGGTGT
TGGACGAGGCCAAGGCGCTGGCCGACGAGCTGCGCGGGCTGGACACGCAG
CTCAGCGTGATCTCGATCCAGGGCGGCGCGCGGACGGCGGAGGAGGACTG
GGCGGCGCGGCAGCTGCTCTCGGCGCTGGTCGCGCCTGGGGACGAGCTGA
TCGAGGTCGACCTGAGCGCCGGGGCGGCGGAGCGGGCG
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