mRNA_M-pyrifera_M_contig107091.1527.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig107091.1527.1
Unique NamemRNA_M-pyrifera_M_contig107091.1527.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig107091.1527.1 vs. uniprot
Match: B8CCD2_THAPS (Protein kinase domain-containing protein n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B8CCD2_THAPS)

HSP 1 Score: 204 bits (519), Expect = 3.630e-62
Identity = 100/160 (62.50%), Postives = 123/160 (76.88%), Query Frame = 1
Query:   10 RGRKIIHYDLKPANVLFDEHRNVKVTDFGLSKIVEDGELGGRGELSGTELTSQGAGTYWYLPPECFEMGPSPPRISSKVDVWSLGVCYFQMLTGRRPYGDGQSQERLLADKIILRASEVNFPHKPTISTLARDFIKKCLTPDQRNRPDIATLLQHPYLRS 489
            R + IIHYDLKP N+LFDEH N K+TDFGLSKIV++  +G      G ELTSQGAGTYWYLPPECF M     RIS+KVDVWS+GV YFQML GRRP+GDG+SQ+R+L ++ +L A+EV+FP KP IS   + FI+ CLT DQR RP+++ L +H YLRS
Sbjct:  196 RRQGIIHYDLKPGNILFDEHGNSKITDFGLSKIVDNEHMG-----EGMELTSQGAGTYWYLPPECFLMDQGV-RISNKVDVWSIGVIYFQMLFGRRPFGDGESQDRVLRNQTMLHATEVHFPSKPLISEEGKMFIRDCLTYDQRIRPNVSQLCEHQYLRS 349          
BLAST of mRNA_M-pyrifera_M_contig107091.1527.1 vs. uniprot
Match: A0A067LFQ5_JATCU (Protein kinase domain-containing protein n=1 Tax=Jatropha curcas TaxID=180498 RepID=A0A067LFQ5_JATCU)

HSP 1 Score: 199 bits (506), Expect = 1.030e-61
Identity = 100/158 (63.29%), Postives = 120/158 (75.95%), Query Frame = 1
Query:   10 RGRKIIHYDLKPANVLFDEHRNVKVTDFGLSKIVEDGELGGRGELSGTELTSQGAGTYWYLPPECFEMGPSPPRISSKVDVWSLGVCYFQMLTGRRPYGDGQSQERLLADKIILRASEVNFPHKPTISTLARDFIKKCLTPDQRNRPDIATLLQHPYL 483
            R +KIIHYDLKP NVLFDE    KVTDFGLSKIVED ++G +G     ELTSQGAGTYWYLPPECFE+   P  ISSKVDVWS GV ++QML GRRP+G  QSQER+L +  I++A  V FP KP++S  A+DFI++CLT +Q  RPD+ T+ Q PYL
Sbjct:   78 RTQKIIHYDLKPGNVLFDELGVAKVTDFGLSKIVED-DVGSQG----MELTSQGAGTYWYLPPECFELSKIP-LISSKVDVWSAGVLFYQMLFGRRPFGHDQSQERILREDTIIKARRVEFPSKPSVSNEAKDFIRRCLTYNQTERPDVLTVAQDPYL 229          
BLAST of mRNA_M-pyrifera_M_contig107091.1527.1 vs. uniprot
Match: A0A7R9YD27_9STRA (Hypothetical protein n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9YD27_9STRA)

HSP 1 Score: 201 bits (512), Expect = 3.120e-61
Identity = 97/157 (61.78%), Postives = 117/157 (74.52%), Query Frame = 1
Query:   22 IIHYDLKPANVLFDEHRNVKVTDFGLSKIVEDGELGGRGELSGTELTSQGAGTYWYLPPECFEMGPSPPRISSKVDVWSLGVCYFQMLTGRRPYGDGQSQERLLADKIILRAS--EVNFPHKPTISTLARDFIKKCLTPDQRNRPDIATLLQHPYLR 486
            IIHYDLKP N+LFD    VK+TDFGLSK++++ E G     +  ELTSQGAGTYWYLPPECF  GP PPRISSKVDV+SLGV Y+QML G+RP GDG +Q++LL   ++LR S  +V FP KP +S  A+DFI+ CL P Q +RPDI  L  HPYLR
Sbjct:  185 IIHYDLKPGNILFDSDGCVKITDFGLSKVIDNSEEG-----TSLELTSQGAGTYWYLPPECFHRGPEPPRISSKVDVFSLGVIYYQMLFGKRPIGDGMTQDQLLGSGLMLRLSGRDVEFPEKPIVSAGAKDFIRACLQPSQIDRPDIRMLCTHPYLR 336          
BLAST of mRNA_M-pyrifera_M_contig107091.1527.1 vs. uniprot
Match: A0A3N6QPC2_BRACR (Protein kinase domain-containing protein n=1 Tax=Brassica cretica TaxID=69181 RepID=A0A3N6QPC2_BRACR)

HSP 1 Score: 197 bits (501), Expect = 5.050e-61
Identity = 98/158 (62.03%), Postives = 116/158 (73.42%), Query Frame = 1
Query:   10 RGRKIIHYDLKPANVLFDEHRNVKVTDFGLSKIVEDGELGGRGELSGTELTSQGAGTYWYLPPECFEMGPSPPRISSKVDVWSLGVCYFQMLTGRRPYGDGQSQERLLADKIILRASEVNFPHKPTISTLARDFIKKCLTPDQRNRPDIATLLQHPYL 483
            R +KIIHYDLKP NVLFDE    KVTDFGLSKIVED          G ELTSQGAGTYWYLPPECFE+  +P  ISSKVDVWS+GV ++QML  +RP+G  QSQER+L +  I++A +V FP KP IS  A+D I++CLT  Q +RPD+ TL Q PYL
Sbjct:   73 RSQKIIHYDLKPGNVLFDEFGVAKVTDFGLSKIVED-----NAGSQGMELTSQGAGTYWYLPPECFELSKTP-MISSKVDVWSVGVLFYQMLFKKRPFGHDQSQERILREDTIIKAKKVEFPAKPAISNEAKDLIRRCLTYSQADRPDVLTLAQDPYL 224          
BLAST of mRNA_M-pyrifera_M_contig107091.1527.1 vs. uniprot
Match: A0A4D9CWT5_9STRA (Protein kinase domain-containing protein n=2 Tax=Monodopsidaceae TaxID=425072 RepID=A0A4D9CWT5_9STRA)

HSP 1 Score: 212 bits (540), Expect = 5.280e-61
Identity = 104/164 (63.41%), Postives = 125/164 (76.22%), Query Frame = 1
Query:    1 GGLRGRKIIHYDLKPANVLFDEHRNVKVTDFGLSKIVEDGELGGRGELSGTELTSQGAGTYWYLPPECFEMGPSPPRISSKVDVWSLGVCYFQMLTGRRPYGDGQSQERLLADKIILRASEVNFPHKPTISTLARDFIKKCLTPDQRNRPDIATLLQHPYLRSK 492
            G  R + IIHYDLKP N+LFDE  +VK+TDFGLSKIV DG   G    S  ELTSQGAGTYWYLPPECF MG +PPRISSKVDVW+LGV +FQML G RP+G+GQ+QE++L +  +LRA+ V+FP KP IS  A++FI+ CLT  Q +RPD+  L  HPYLR K
Sbjct:  787 GANRRQGIIHYDLKPGNILFDEQGDVKITDFGLSKIV-DGSCDG----SSLELTSQGAGTYWYLPPECFVMGSTPPRISSKVDVWALGVIFFQMLFGSRPFGEGQTQEKVLHESTMLRATRVSFPAKPPISADAKEFIRACLTHSQADRPDVLKLCAHPYLRLK 945          
BLAST of mRNA_M-pyrifera_M_contig107091.1527.1 vs. uniprot
Match: A0A1D6GH13_MAIZE (Serine/threonine-protein kinase TOUSLED n=1 Tax=Zea mays TaxID=4577 RepID=A0A1D6GH13_MAIZE)

HSP 1 Score: 194 bits (493), Expect = 3.540e-60
Identity = 97/158 (61.39%), Postives = 118/158 (74.68%), Query Frame = 1
Query:   10 RGRKIIHYDLKPANVLFDEHRNVKVTDFGLSKIVEDGELGGRGELSGTELTSQGAGTYWYLPPECFEMGPSPPRISSKVDVWSLGVCYFQMLTGRRPYGDGQSQERLLADKIILRASEVNFPHKPTISTLARDFIKKCLTPDQRNRPDIATLLQHPYL 483
            RG+KIIHYDLKP NVLFDE    KVTDFGLSKIVED ++G +G     ELTSQGAGTYWYLPPECF++  +P  ISSKVDVWS GV ++QML GRRP+G  Q+QER+L +  I+ A  V FP KP +S  A+D I++CLT +Q  RPD+ T+ Q  YL
Sbjct:   46 RGQKIIHYDLKPGNVLFDEVGVAKVTDFGLSKIVED-DVGSQG----MELTSQGAGTYWYLPPECFDLSKTP-FISSKVDVWSAGVMFYQMLYGRRPFGHDQTQERILREDTIINARRVEFPSKPAVSNEAKDLIRRCLTYNQSERPDVLTITQDHYL 197          
BLAST of mRNA_M-pyrifera_M_contig107091.1527.1 vs. uniprot
Match: A0A5B6ZZW0_DAVIN (Protein kinase domain-containing protein (Fragment) n=2 Tax=Pentapetalae TaxID=1437201 RepID=A0A5B6ZZW0_DAVIN)

HSP 1 Score: 194 bits (494), Expect = 4.830e-60
Identity = 96/158 (60.76%), Postives = 120/158 (75.95%), Query Frame = 1
Query:   10 RGRKIIHYDLKPANVLFDEHRNVKVTDFGLSKIVEDGELGGRGELSGTELTSQGAGTYWYLPPECFEMGPSPPRISSKVDVWSLGVCYFQMLTGRRPYGDGQSQERLLADKIILRASEVNFPHKPTISTLARDFIKKCLTPDQRNRPDIATLLQHPYL 483
            R +KIIH+DLKP NVLFDE    KVTDFGLSKIVED ++G +G     ELTSQGAGTYWYLPPECFE+  +P  ISSKVDVWS G+  +QML GRRP+G  Q+QER+L +  I++A +V FP +P +S  A+DFI++CLT +Q  RPD+ T+ Q PYL
Sbjct:   67 RAQKIIHFDLKPGNVLFDELGVAKVTDFGLSKIVED-DVGSQG----MELTSQGAGTYWYLPPECFELSKTP-LISSKVDVWSAGILLYQMLFGRRPFGHDQTQERILREDTIIKARKVEFPSRPAVSNEAKDFIRRCLTYNQAERPDVLTIGQDPYL 218          
BLAST of mRNA_M-pyrifera_M_contig107091.1527.1 vs. uniprot
Match: A0A835YR98_9STRA (Kinase-like domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YR98_9STRA)

HSP 1 Score: 199 bits (507), Expect = 5.120e-60
Identity = 95/157 (60.51%), Postives = 120/157 (76.43%), Query Frame = 1
Query:   22 IIHYDLKPANVLFDEHRNVKVTDFGLSKIVEDGELGGRGELSGTELTSQGAGTYWYLPPECFEMGPSPPRISSKVDVWSLGVCYFQMLTGRRPYGDGQSQERLLADKIILRASEVNFPHKPTISTLARDFIKKCLTPDQRNRPDIATLLQHPYLRSK 492
            IIHYDLKPANVLFD   +VK+TDFGLSKIVE  +     E  G ELTSQGAGT WYLPPECF MG + PRIS KVDVWS+GV +FQML G+RP+G+G+ Q+++L D+ +LRA++V+FP KP  S  A+ FI+ CL   Q +RPD+ TL  HPY+R++
Sbjct:  231 IIHYDLKPANVLFDGAGDVKITDFGLSKIVEAEQ-----ESGGVELTSQGAGTLWYLPPECFVMGGAAPRISGKVDVWSVGVIFFQMLYGQRPFGEGKDQQKVLHDQTMLRATQVDFPPKPAASAEAKAFIRLCLAHAQEDRPDVRTLCAHPYIRAR 382          
BLAST of mRNA_M-pyrifera_M_contig107091.1527.1 vs. uniprot
Match: A0A1D6GH56_MAIZE (Serine/threonine-protein kinase TOUSLED n=1 Tax=Zea mays TaxID=4577 RepID=A0A1D6GH56_MAIZE)

HSP 1 Score: 194 bits (493), Expect = 3.410e-59
Identity = 97/158 (61.39%), Postives = 118/158 (74.68%), Query Frame = 1
Query:   10 RGRKIIHYDLKPANVLFDEHRNVKVTDFGLSKIVEDGELGGRGELSGTELTSQGAGTYWYLPPECFEMGPSPPRISSKVDVWSLGVCYFQMLTGRRPYGDGQSQERLLADKIILRASEVNFPHKPTISTLARDFIKKCLTPDQRNRPDIATLLQHPYL 483
            RG+KIIHYDLKP NVLFDE    KVTDFGLSKIVED ++G +G     ELTSQGAGTYWYLPPECF++  +P  ISSKVDVWS GV ++QML GRRP+G  Q+QER+L +  I+ A  V FP KP +S  A+D I++CLT +Q  RPD+ T+ Q  YL
Sbjct:  120 RGQKIIHYDLKPGNVLFDEVGVAKVTDFGLSKIVED-DVGSQG----MELTSQGAGTYWYLPPECFDLSKTP-FISSKVDVWSAGVMFYQMLYGRRPFGHDQTQERILREDTIINARRVEFPSKPAVSNEAKDLIRRCLTYNQSERPDVLTITQDHYL 271          
BLAST of mRNA_M-pyrifera_M_contig107091.1527.1 vs. uniprot
Match: A0A2N9IGK7_FAGSY (Protein kinase domain-containing protein n=1 Tax=Fagus sylvatica TaxID=28930 RepID=A0A2N9IGK7_FAGSY)

HSP 1 Score: 191 bits (486), Expect = 5.260e-59
Identity = 93/158 (58.86%), Postives = 119/158 (75.32%), Query Frame = 1
Query:   10 RGRKIIHYDLKPANVLFDEHRNVKVTDFGLSKIVEDGELGGRGELSGTELTSQGAGTYWYLPPECFEMGPSPPRISSKVDVWSLGVCYFQMLTGRRPYGDGQSQERLLADKIILRASEVNFPHKPTISTLARDFIKKCLTPDQRNRPDIATLLQHPYL 483
            R +KIIHYDLKP NVLFDE    KVTDFGLSKIVE+  +G +G     ELTSQGAGTYWYLPPECFE+  +P  ISSKVDVWS G+ ++QML GRRP+G  Q+QER+L +  I++A +V FP +P +S  A++ I++C+T +Q  RPD+ T+ Q PYL
Sbjct:   54 RTQKIIHYDLKPGNVLFDEFGVAKVTDFGLSKIVEE-NVGSQG----MELTSQGAGTYWYLPPECFELSKTP-LISSKVDVWSAGILFYQMLFGRRPFGHDQTQERILREDTIIKARKVEFPSRPAVSNEAKELIRRCVTYNQAERPDVLTIAQDPYL 205          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig107091.1527.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
B8CCD2_THAPS3.630e-6262.50Protein kinase domain-containing protein n=1 Tax=T... [more]
A0A067LFQ5_JATCU1.030e-6163.29Protein kinase domain-containing protein n=1 Tax=J... [more]
A0A7R9YD27_9STRA3.120e-6161.78Hypothetical protein n=1 Tax=Pinguiococcus pyrenoi... [more]
A0A3N6QPC2_BRACR5.050e-6162.03Protein kinase domain-containing protein n=1 Tax=B... [more]
A0A4D9CWT5_9STRA5.280e-6163.41Protein kinase domain-containing protein n=2 Tax=M... [more]
A0A1D6GH13_MAIZE3.540e-6061.39Serine/threonine-protein kinase TOUSLED n=1 Tax=Ze... [more]
A0A5B6ZZW0_DAVIN4.830e-6060.76Protein kinase domain-containing protein (Fragment... [more]
A0A835YR98_9STRA5.120e-6060.51Kinase-like domain-containing protein n=1 Tax=Trib... [more]
A0A1D6GH56_MAIZE3.410e-5961.39Serine/threonine-protein kinase TOUSLED n=1 Tax=Ze... [more]
A0A2N9IGK7_FAGSY5.260e-5958.86Protein kinase domain-containing protein n=1 Tax=F... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig107091contigM-pyrifera_M_contig107091:53..553 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score204.9
Seed ortholog evalue2.9e-50
Seed eggNOG ortholog35128.Thaps37523
Preferred nameTLK2
KEGG koko:K08864
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0004672,GO:0004674,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0006464,GO:0006468,GO:0006793,GO:0006796,GO:0006807,GO:0008150,GO:0008152,GO:0009507,GO:0009536,GO:0009987,GO:0010468,GO:0016301,GO:0016310,GO:0016740,GO:0016772,GO:0016773,GO:0019222,GO:0019538,GO:0036211,GO:0042802,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043412,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044424,GO:0044444,GO:0044464,GO:0050789,GO:0050794,GO:0060147,GO:0060255,GO:0060966,GO:0060968,GO:0065007,GO:0071704,GO:0140096,GO:1900368,GO:1901564
EggNOG free text desc.Belongs to the protein kinase superfamily
EggNOG OGs2XB28@2836,KOG1151@1,KOG1151@2759
EC2.7.11.1
COG Functional cat.T
Best tax levelBacillariophyta
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko01001
Exons1
Model size501
Cds size501
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815581.2986662-CDS-M-pyrifera_M_contig107091:52..5531622815581.2986662-CDS-M-pyrifera_M_contig107091:52..553Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig107091 53..553 +
1692277473.5279222-CDS-M-pyrifera_M_contig107091:52..5531692277473.5279222-CDS-M-pyrifera_M_contig107091:52..553Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig107091 53..553 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig107091.1527.1prot_M-pyrifera_M_contig107091.1527.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig107091 53..553 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig107091.1527.1

>prot_M-pyrifera_M_contig107091.1527.1 ID=prot_M-pyrifera_M_contig107091.1527.1|Name=mRNA_M-pyrifera_M_contig107091.1527.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=167bp
GGLRGRKIIHYDLKPANVLFDEHRNVKVTDFGLSKIVEDGELGGRGELSG
TELTSQGAGTYWYLPPECFEMGPSPPRISSKVDVWSLGVCYFQMLTGRRP
YGDGQSQERLLADKIILRASEVNFPHKPTISTLARDFIKKCLTPDQRNRP
DIATLLQHPYLRSKPK*
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mRNA from alignment at M-pyrifera_M_contig107091:53..553+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig107091.1527.1 ID=mRNA_M-pyrifera_M_contig107091.1527.1|Name=mRNA_M-pyrifera_M_contig107091.1527.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=501bp|location=Sequence derived from alignment at M-pyrifera_M_contig107091:53..553+ (Macrocystis pyrifera P11B4 male)
GGTGGATTGCGTGGGAGAAAAATTATTCATTATGATTTGAAACCTGCGAA TGTTTTGTTTGATGAACATCGCAATGTGAAAGTCACAGATTTTGGATTGA GCAAGATTGTGGAGGATGGAGAACTTGGAGGGAGGGGAGAATTGAGCGGA ACGGAACTCACATCACAGGGTGCCGGAACATATTGGTATCTCCCACCAGA ATGCTTTGAAATGGGACCATCTCCTCCTCGAATCTCTAGTAAAGTAGATG TTTGGAGCTTGGGGGTGTGCTACTTTCAAATGCTCACAGGAAGAAGGCCA TATGGAGATGGACAAAGTCAAGAAAGACTCCTTGCAGATAAAATTATCCT TCGAGCTTCGGAAGTCAACTTTCCACATAAGCCGACCATCTCCACCCTTG CAAGAGATTTTATCAAGAAATGCCTCACACCAGATCAAAGAAACAGACCA GATATTGCCACTCTTCTACAACACCCATACCTCCGGTCCAAACCAAAGTA G
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig107091:53..553+

>mRNA_M-pyrifera_M_contig107091.1527.1 ID=mRNA_M-pyrifera_M_contig107091.1527.1|Name=mRNA_M-pyrifera_M_contig107091.1527.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1002bp|location=Sequence derived from alignment at M-pyrifera_M_contig107091:53..553+ (Macrocystis pyrifera P11B4 male)
GGTGGATTGCGTGGGAGAAAAATTATTCATTATGATTTGAAACCTGCGAA
TGTTTTGTTTGATGAACATCGCAATGTGAAAGTCACAGATTTTGGATTGA
GCAAGATTGTGGAGGATGGAGAACTTGGAGGGAGGGGAGAATTGAGCGGA
ACGGAACTCACATCACAGGGTGCCGGAACATATTGGTATCTCCCACCAGA
ATGCTTTGAAATGGGACCATCTCCTCCTCGAATCTCTAGTAAAGTAGATG
TTTGGAGCTTGGGGGTGTGCTACTTTCAAATGCTCACAGGAAGAAGGCCA
TATGGAGATGGACAAAGTCAAGAAAGACTCCTTGCAGATAAAATTATCCT
TCGAGCTTCGGAAGTCAACTTTCCACATAAGCCGACCATCTCCACCCTTG
CAAGAGATTTTATCAAGAAATGCCTCACACCAGATCAAAGAAACAGACCA
GATATTGCCACTCTTCTACAACACCCATACCTCCGGTCCAAACCAAAGTA
GGGTGGATTGCGTGGGAGAAAAATTATTCATTATGATTTGAAACCTGCGA
ATGTTTTGTTTGATGAACATCGCAATGTGAAAGTCACAGATTTTGGATTG
AGCAAGATTGTGGAGGATGGAGAACTTGGAGGGAGGGGAGAATTGAGCGG
AACGGAACTCACATCACAGGGTGCCGGAACATATTGGTATCTCCCACCAG
AATGCTTTGAAATGGGACCATCTCCTCCTCGAATCTCTAGTAAAGTAGAT
GTTTGGAGCTTGGGGGTGTGCTACTTTCAAATGCTCACAGGAAGAAGGCC
ATATGGAGATGGACAAAGTCAAGAAAGACTCCTTGCAGATAAAATTATCC
TTCGAGCTTCGGAAGTCAACTTTCCACATAAGCCGACCATCTCCACCCTT
GCAAGAGATTTTATCAAGAAATGCCTCACACCAGATCAAAGAAACAGACC
AGATATTGCCACTCTTCTACAACACCCATACCTCCGGTCCAAACCAAAGT
AG
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