mRNA_M-pyrifera_M_contig107078.1520.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig107078.1520.1
Unique NamemRNA_M-pyrifera_M_contig107078.1520.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig107078.1520.1 vs. uniprot
Match: A0A5A8CCB5_CAFRO (Kri1_C domain-containing protein n=5 Tax=Sar TaxID=2698737 RepID=A0A5A8CCB5_CAFRO)

HSP 1 Score: 111 bits (278), Expect = 4.480e-25
Identity = 79/198 (39.90%), Postives = 112/198 (56.57%), Query Frame = 1
Query:   10 LVVDKKFADYYDTSRRRRELARARELGLL--AEEDSDRESG----DDGSSSEEEDEGAVPLETDLKMMRLISMIRKRDPRIYDANARWFDENDAMGPGAVENEGEKEAGGDSKRMGDVVRDQVVRAMEEGKEVAFDEEXXXXXXXXXXXX------------RVGRRSEAGLRALAYDEEQRKLRAAFLESAKKSFGA 549
            L V+K+FA+++D SRRRR+LARARE G+L  AE    R  G    DD S    EDEG V   TD+++MR+I  IR +DP++Y+    WF E+DA+G   V+     EAG   +R  DV+R+QVV+A+ EG+  AF ++                        R+   SEA  R + Y+EEQ  +R AFLE+ ++S  A
Sbjct:   24 LRVNKQFAEHFDASRRRRDLARAREQGMLHEAERRIARMDGVAADDDDSDESSEDEGHVEPATDMRIMRVIQAIRNKDPKVYEDGVAWFKEDDALGKPDVD-----EAGHTRQRPADVIREQVVKAVREGRSDAFGDDDGPERPTGKGDGEDSDSGDEGTGNRLAAASEAAKRKV-YNEEQAAMRQAFLEAVRESADA 215          
BLAST of mRNA_M-pyrifera_M_contig107078.1520.1 vs. uniprot
Match: A0A812VPQ9_9DINO (KRI1 protein n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A812VPQ9_9DINO)

HSP 1 Score: 82.4 bits (202), Expect = 1.190e-15
Identity = 76/196 (38.78%), Postives = 109/196 (55.61%), Query Frame = 1
Query:   10 LVVDKKFADYYDTSRRRRELARARELGLLAEEDSDRESGDDGSSSEEEDEGAV-PLETDLKMMRLISMIRKRDPRIYDANARWFDENDAMGPGAVENEGEKEAGGDSKRMG-------DVVRDQVVRAMEEGKEVAFDEEXXXXXXXXXXXXRVGRRSEAGLRALAYDEEQRKLRAAFLESAKKSF--GAGGGDDE 567
            L V+++FA+ ++  +R++ELA+ARE GL+  + +  ++ D+ SS  E+D+GA+   ETDL +++ +  IR ++P IY  +  WF E D        +E E EA    KR         DV+  QV  A   GK   FDEE  XXXXXXXXXX     +        YDEEQ++LR  FL+S KK     +G  DDE
Sbjct:   16 LRVNERFAEDFERRKRKQELAKAREAGLIDADGNIIQASDESSSESEDDDGALLSPETDLHIIKTLEAIRSKNPAIYKKDQAWFTEGDDSS-----DEAEGEANTAGKRQSRKKVTARDVIGQQVAEAAALGKSEVFDEEGEXXXXXXXXXXXXAPVN-------MYDEEQKRLREEFLKSVKKEAEDDSGSSDDE 199          
BLAST of mRNA_M-pyrifera_M_contig107078.1520.1 vs. uniprot
Match: A0A7S2CHA4_9STRA (Hypothetical protein (Fragment) n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2CHA4_9STRA)

HSP 1 Score: 74.3 bits (181), Expect = 3.450e-12
Identity = 63/186 (33.87%), Postives = 91/186 (48.92%), Query Frame = 1
Query:   10 LVVDKKFADYYDTSRRRRELARARELGLLAEEDSDRESGDDGSSSEEEDEGAVPL-ETDLKMMRLISMIRKRDPRIYDANARWFDENDAMGPGAVENEGEKEAGGDSKRMGDVVRDQVVRAMEEGKEVAFDEEXXXXXXXXXXXXRVGRRSEAGLRALAYDEEQRKLRAAFLESAKKSFGAGGGDD 564
            + V++ +A+ Y+ S+RR ELA      LL + DSD    D+  SSE EDE  +     D ++ + I  IR RDP +Y+ +A+WFDEN A G     +   ++     K   DV+R+QV+   E     A  E                  SE G +  AYD+EQR LR AFL +      + GG+D
Sbjct:   19 IQVNQAYAERYERSKRRTELANQDTKNLLRQVDSD----DESESSESEDEAVLADGRIDCQIAKTIESIRNRDPEVYNPDAQWFDEN-AEGSDDESDRKPRDQKKQKKFFKDVIREQVLEDAETLGHAAPGEMTTGTHDKNE--------SETG-KITAYDDEQRGLRQAFLTADDSDASSSGGED 190          
BLAST of mRNA_M-pyrifera_M_contig107078.1520.1 vs. uniprot
Match: A0A7S2G327_9STRA (Hypothetical protein n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2G327_9STRA)

HSP 1 Score: 74.3 bits (181), Expect = 3.890e-12
Identity = 63/186 (33.87%), Postives = 91/186 (48.92%), Query Frame = 1
Query:   10 LVVDKKFADYYDTSRRRRELARARELGLLAEEDSDRESGDDGSSSEEEDEGAVPL-ETDLKMMRLISMIRKRDPRIYDANARWFDENDAMGPGAVENEGEKEAGGDSKRMGDVVRDQVVRAMEEGKEVAFDEEXXXXXXXXXXXXRVGRRSEAGLRALAYDEEQRKLRAAFLESAKKSFGAGGGDD 564
            + V++ +A+ Y+ S+RR ELA      LL + DSD    D+  SSE EDE  +     D ++ + I  IR RDP +Y+ +A+WFDEN A G     +   ++     K   DV+R+QV+   E     A  E                  SE G +  AYD+EQR LR AFL +      + GG+D
Sbjct:   19 IQVNQAYAERYERSKRRTELANQDTKNLLRQVDSD----DESESSESEDEAVLADGRIDCQIAKTIESIRNRDPEVYNPDAQWFDEN-AEGSDDESDRKPRDQKKQKKFFKDVIREQVLEDAETLGHAAPGEMTTGTHDKNE--------SETG-KITAYDDEQRGLRQAFLTADDSDASSSGGED 190          
BLAST of mRNA_M-pyrifera_M_contig107078.1520.1 vs. uniprot
Match: A0A7S2VCY1_9STRA (Hypothetical protein n=2 Tax=Amphiprora paludosa TaxID=265537 RepID=A0A7S2VCY1_9STRA)

HSP 1 Score: 73.2 bits (178), Expect = 1.010e-11
Identity = 64/181 (35.36%), Postives = 94/181 (51.93%), Query Frame = 1
Query:   10 LVVDKKFADYYDTSRRRRELARARELGLLAEEDSDRESGDDGSSSEEEDEGAVPLETDLKMMRLISMIRKRDPRIYDANARWFD----ENDAMGPGAVENEGEKEAGGDSKRMGDVVRDQVVRAMEEGKE-VAFDEEXXXXXXXXXXXXRVGRRSEAGLRALAYDEEQRKLRAAFLESAKK 537
            L+++KKFA  YD  +++ EL  AR  G   EEDS+    DD +  E+ D     L+TD+  M+ ++ +R +D  IY+   R+FD      D  G  + E  G K+     KR  DVVR+Q++  ME+G   +A D +            +   RSE     LAYD+EQR++R AFL S  K
Sbjct:   21 LLINKKFAKEYDARKQKLELRTARLRGEAPEEDSE----DDETEDEDGDLLTTRLDTDI--MKTLNALRSKDETIYNPETRFFDGTKPSEDDEGEDSDEELGRKDK---PKRYKDVVREQILEEMEKGTSAIAHDGDNDDKDEGK----KQSYRSE-----LAYDDEQREIRKAFLSSTTK 183          
BLAST of mRNA_M-pyrifera_M_contig107078.1520.1 vs. uniprot
Match: B7FUH6_PHATC (Predicted protein n=2 Tax=Phaeodactylum tricornutum TaxID=2850 RepID=B7FUH6_PHATC)

HSP 1 Score: 67.0 bits (162), Expect = 1.320e-9
Identity = 55/185 (29.73%), Postives = 92/185 (49.73%), Query Frame = 1
Query:   16 VDKKFADYYDTSRRRRELARARELGLLAEEDSDRESGDDGSSSEEEDEGAVPLETDLKMMRLISMIRKRDPRIYDANARWFDENDAMGPGAVENEGEKEAGGDSKRMGDVVRDQVVRAMEEGKEVAFDEEXXXXXXXXXXXXRVGRRSEAGLRALAYDEEQRKLRAAFLESAKKSFGAGGGDDEE 570
            V+ K+A  Y++ +++ EL R +  G       D + G+   SSE+ED   +  + D  +++ I+++R +D RIYD +  +FD  +     ++     KE+    KR  DVVR+Q+++ M+E  +V   EE                 S+     LAYD  Q++LR AF++S     G G  DD E
Sbjct:   26 VNTKYAKEYESRKQKEELRRVQFQG-------DDDDGESTDSSEDEDAELLTAKLDTNIIKTINILRSKDSRIYDPSVNFFDNAEDSEEDSLPT---KESKSKPKRYKDVVREQILKQMDE--DVPIGEENDNDYGVLA--------SDEAKSRLAYDNRQQELRKAFVDSTTGKKGYGIDDDGE 190          
BLAST of mRNA_M-pyrifera_M_contig107078.1520.1 vs. uniprot
Match: A0A1E7EVN5_9STRA (Krr1-domain-containing protein (Fragment) n=1 Tax=Fragilariopsis cylindrus CCMP1102 TaxID=635003 RepID=A0A1E7EVN5_9STRA)

HSP 1 Score: 63.2 bits (152), Expect = 2.780e-8
Identity = 45/130 (34.62%), Postives = 71/130 (54.62%), Query Frame = 1
Query:  184 LKMMRLISMIRKRDPRIYDANARWFDENDAMGPGAVENEGEKEAGGDSKRMGDVVRDQVVRAMEEGKEVAFDEEXXXXXXXXXXXXRVGRRSEAGLRALAYDEEQRKLRAAFL-ESAKKSFGAGGGDDEE 570
            ++ ++ I  +RK+D +IYD N+R+FD+++    G  +N+G+ E     KR  DVVR+Q++  ME       DEE             +   S+     L YDE+Q KLR AFL ES++K+ G    +D+E
Sbjct:   70 VQFLKTIKALRKKDDKIYDPNSRFFDDDNYNAVGDDKNDGDTEKKQKPKRYKDVVREQILEQME-------DEENEGGNKKTNTNVDM---SDDHRSKLGYDEQQNKLRKAFLTESSEKTDGEKSDEDDE 189          
BLAST of mRNA_M-pyrifera_M_contig107078.1520.1 vs. uniprot
Match: A0A4D9CR12_9STRA (Kri1_C domain-containing protein n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9CR12_9STRA)

HSP 1 Score: 61.2 bits (147), Expect = 1.270e-7
Identity = 54/183 (29.51%), Postives = 92/183 (50.27%), Query Frame = 1
Query:   10 LVVDKKFADYYDTSRRRRELARARELGLLAEEDSDRESGD-DGSSSE--------EEDEGAVPLETDLKMMRLISMIRKRDPRIYDANARWFDENDAMGPGAVENEGEKEAGGDSKRMGDVVRDQVVRAMEEGKEVAFDEEXXXXXXXXXXXXRVGRRSEAGLRALAYDEEQRKLRAAFLESA 531
            L V++ +A ++++S++++ELA+ R    ++   SD  S + +G  S+         E E   P   +++++R I+ IR+RDP+IY  + +WF   +       E +  +  G   KR  DV+R+QV+ A+E  KE     E            R G       + + YD EQ +LR AFLE+A
Sbjct:   27 LRVNRVYASHFESSKKKQELAQLRRQQHVSWGASDIVSDEGEGEGSDVSXXXXXXXEGEELTPA-MEVQILRTINSIRERDPKIYQHDMKWFSNGEDE---EAEGKEGRRKGERPKRYKDVIREQVLAAVEADKEAIESSEEEDLDKSGAGGERGG--EPVSSQRMGYDAEQLQLRQAFLEAA 203          
BLAST of mRNA_M-pyrifera_M_contig107078.1520.1 vs. uniprot
Match: B8C2I4_THAPS (Uncharacterized protein (Fragment) n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B8C2I4_THAPS)

HSP 1 Score: 56.2 bits (134), Expect = 6.120e-6
Identity = 51/179 (28.49%), Postives = 83/179 (46.37%), Query Frame = 1
Query:   10 LVVDKKFADYYDTSRRRRELARARELGLLAEEDSDRESGDDGSSSEEEDEGAVPLETDLKMMRLISMIRKRDPRIYDANARWFDENDAMGPGAVENEGEKEAGGDSKRMGDVVRDQVVRAME--EGKEVAFDEEXXXXXXXXXXXXRVGRRSEAGLRALAYDEEQRKLRAAFLESAKKS 540
            + +++K+A  Y+  +RR EL   R +    EED   +S         E E   P   D+K+++ +  ++ +D  IYD N  +F +ND+      E    K      KR  D+VR+Q++  ME  EGK++   ++                        LAYD+EQR +R AFL+S + S
Sbjct:   50 ININQKYAREYEVRKRREELQNNRNIN---EEDGISDSXXXXXXXXXEAELLTP-HMDVKIIKTLRALKSKDEGIYDQNVAFFSDNDSESGDDDETGDGKRDKTKPKRYKDLVREQILEQMESGEGKDLGDTDKAQGPEAHN----------------LAYDQEQRDIRRAFLDSTRDS 208          
BLAST of mRNA_M-pyrifera_M_contig107078.1520.1 vs. uniprot
Match: A0A5J4W9V9_9EUKA (Putative krr family protein n=1 Tax=Streblomastix strix TaxID=222440 RepID=A0A5J4W9V9_9EUKA)

HSP 1 Score: 56.2 bits (134), Expect = 6.360e-6
Identity = 30/84 (35.71%), Postives = 52/84 (61.90%), Query Frame = 1
Query:   10 LVVDKKFADYYDTSRRRRELARARELGLLAEEDSDRESGDDGSSSEEEDEGAVPLETDLKMMRLISMIRKRDPRIYDANARWFD 261
            + +++++A  ++  +R+ +LARA + GLL E         D   +EE++   V  E DL+++ +I  IRK+DP IYD NA++FD
Sbjct:  169 MNINQEYARKFEERKRKEDLARAADRGLLDE---------DSEQTEEDEAEDVNPEVDLQILDVIQRIRKKDPSIYDPNAKFFD 243          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig107078.1520.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 14
Match NameE-valueIdentityDescription
A0A5A8CCB5_CAFRO4.480e-2539.90Kri1_C domain-containing protein n=5 Tax=Sar TaxID... [more]
A0A812VPQ9_9DINO1.190e-1538.78KRI1 protein n=1 Tax=Symbiodinium sp. KB8 TaxID=23... [more]
A0A7S2CHA4_9STRA3.450e-1233.87Hypothetical protein (Fragment) n=1 Tax=Dictyocha ... [more]
A0A7S2G327_9STRA3.890e-1233.87Hypothetical protein n=1 Tax=Dictyocha speculum Ta... [more]
A0A7S2VCY1_9STRA1.010e-1135.36Hypothetical protein n=2 Tax=Amphiprora paludosa T... [more]
B7FUH6_PHATC1.320e-929.73Predicted protein n=2 Tax=Phaeodactylum tricornutu... [more]
A0A1E7EVN5_9STRA2.780e-834.62Krr1-domain-containing protein (Fragment) n=1 Tax=... [more]
A0A4D9CR12_9STRA1.270e-729.51Kri1_C domain-containing protein n=1 Tax=Nannochlo... [more]
B8C2I4_THAPS6.120e-628.49Uncharacterized protein (Fragment) n=1 Tax=Thalass... [more]
A0A5J4W9V9_9EUKA6.360e-635.71Putative krr family protein n=1 Tax=Streblomastix ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig107078contigM-pyrifera_M_contig107078:22..591 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score69.3
Seed ortholog evalue2.2e-09
Seed eggNOG ortholog2850.Phatr44555
KEGG koko:K10592,ko:K14786
KEGG Pathwayko04120,map04120
Hectar predicted targeting categoryother localisation
EggNOG free text desc.KRI1-like family
EggNOG OGs2XAJD@2836,KOG2409@1,KOG2409@2759
EC2.3.2.26
COG Functional cat.J
Best tax levelBacillariophyta
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03009,ko04121
Exons1
Model size570
Cds size570
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815581.0317783-CDS-M-pyrifera_M_contig107078:21..5911622815581.0317783-CDS-M-pyrifera_M_contig107078:21..591Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig107078 22..591 -
1692277473.429351-CDS-M-pyrifera_M_contig107078:21..5911692277473.429351-CDS-M-pyrifera_M_contig107078:21..591Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig107078 22..591 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig107078.1520.1prot_M-pyrifera_M_contig107078.1520.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig107078 22..591 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig107078.1520.1

>prot_M-pyrifera_M_contig107078.1520.1 ID=prot_M-pyrifera_M_contig107078.1520.1|Name=mRNA_M-pyrifera_M_contig107078.1520.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=190bp
MGGLVVDKKFADYYDTSRRRRELARARELGLLAEEDSDRESGDDGSSSEE
EDEGAVPLETDLKMMRLISMIRKRDPRIYDANARWFDENDAMGPGAVENE
GEKEAGGDSKRMGDVVRDQVVRAMEEGKEVAFDEEDEEDEEEEGGGGRVG
RRSEAGLRALAYDEEQRKLRAAFLESAKKSFGAGGGDDEE
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mRNA from alignment at M-pyrifera_M_contig107078:22..591-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig107078.1520.1 ID=mRNA_M-pyrifera_M_contig107078.1520.1|Name=mRNA_M-pyrifera_M_contig107078.1520.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=570bp|location=Sequence derived from alignment at M-pyrifera_M_contig107078:22..591- (Macrocystis pyrifera P11B4 male)
ATGGGTGGTTTGGTGGTGGACAAAAAGTTTGCTGACTACTATGACACGTC TCGTCGTCGTCGTGAGTTGGCACGCGCTCGGGAGTTGGGTTTGCTTGCAG AGGAAGATAGTGATAGGGAGAGTGGGGATGATGGTAGCTCTAGCGAAGAG GAGGATGAAGGTGCCGTACCTCTGGAGACTGATCTGAAGATGATGCGCCT AATTTCCATGATTCGCAAGCGAGACCCACGCATCTATGATGCTAATGCGC GTTGGTTTGATGAAAATGACGCAATGGGTCCAGGGGCGGTGGAAAATGAG GGTGAAAAGGAAGCTGGGGGTGATTCGAAGCGAATGGGGGATGTAGTGCG TGACCAGGTTGTGCGAGCGATGGAAGAAGGCAAAGAAGTGGCTTTTGATG AGGAAGATGAGGAAGATGAGGAGGAAGAAGGGGGTGGTGGAAGGGTGGGA AGGAGAAGTGAAGCTGGATTGAGAGCTTTGGCGTATGATGAGGAACAACG AAAGTTGAGAGCTGCATTTTTGGAGAGTGCAAAGAAGAGTTTTGGAGCTG GTGGTGGGGATGATGAGGAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig107078:22..591-

>mRNA_M-pyrifera_M_contig107078.1520.1 ID=mRNA_M-pyrifera_M_contig107078.1520.1|Name=mRNA_M-pyrifera_M_contig107078.1520.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1140bp|location=Sequence derived from alignment at M-pyrifera_M_contig107078:22..591- (Macrocystis pyrifera P11B4 male)
ATGGGTGGTTTGGTGGTGGACAAAAAGTTTGCTGACTACTATGACACGTC
TCGTCGTCGTCGTGAGTTGGCACGCGCTCGGGAGTTGGGTTTGCTTGCAG
AGGAAGATAGTGATAGGGAGAGTGGGGATGATGGTAGCTCTAGCGAAGAG
GAGGATGAAGGTGCCGTACCTCTGGAGACTGATCTGAAGATGATGCGCCT
AATTTCCATGATTCGCAAGCGAGACCCACGCATCTATGATGCTAATGCGC
GTTGGTTTGATGAAAATGACGCAATGGGTCCAGGGGCGGTGGAAAATGAG
GGTGAAAAGGAAGCTGGGGGTGATTCGAAGCGAATGGGGGATGTAGTGCG
TGACCAGGTTGTGCGAGCGATGGAAGAAGGCAAAGAAGTGGCTTTTGATG
AGGAAGATGAGGAAGATGAGGAGGAAGAAGGGGGTGGTGGAAGGGTGGGA
AGGAGAAGTGAAGCTGGATTGAGAGCTTTGGCGTATGATGAGGAACAACG
AAAGTTGAGAGCTGCATTTTTGGAGAGTGCAAAGAAGAGTTTTGGAGCTG
GTGGTGGGGATGATGAGGAGATGGGTGGTTTGGTGGTGGACAAAAAGTTT
GCTGACTACTATGACACGTCTCGTCGTCGTCGTGAGTTGGCACGCGCTCG
GGAGTTGGGTTTGCTTGCAGAGGAAGATAGTGATAGGGAGAGTGGGGATG
ATGGTAGCTCTAGCGAAGAGGAGGATGAAGGTGCCGTACCTCTGGAGACT
GATCTGAAGATGATGCGCCTAATTTCCATGATTCGCAAGCGAGACCCACG
CATCTATGATGCTAATGCGCGTTGGTTTGATGAAAATGACGCAATGGGTC
CAGGGGCGGTGGAAAATGAGGGTGAAAAGGAAGCTGGGGGTGATTCGAAG
CGAATGGGGGATGTAGTGCGTGACCAGGTTGTGCGAGCGATGGAAGAAGG
CAAAGAAGTGGCTTTTGATGAGGAAGATGAGGAAGATGAGGAGGAAGAAG
GGGGTGGTGGAAGGGTGGGAAGGAGAAGTGAAGCTGGATTGAGAGCTTTG
GCGTATGATGAGGAACAACGAAAGTTGAGAGCTGCATTTTTGGAGAGTGC
AAAGAAGAGTTTTGGAGCTGGTGGTGGGGATGATGAGGAG
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