mRNA_M-pyrifera_M_contig106162.1337.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig106162.1337.1
Unique NamemRNA_M-pyrifera_M_contig106162.1337.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig106162.1337.1 vs. uniprot
Match: F0ZYD6_DICPU (eIF-2B GDP-GTP exchange factor subunit epsilon n=1 Tax=Dictyostelium purpureum TaxID=5786 RepID=F0ZYD6_DICPU)

HSP 1 Score: 142 bits (357), Expect = 2.180e-37
Identity = 61/96 (63.54%), Postives = 80/96 (83.33%), Query Frame = 1 
Query:    1 IFKEHHAVEFRYDLIDCNIDICSPEVLFLFQDNFDYKDLRRDFINGVLGSELLGYELYTYVIDKEYAARVRGLNTYAAVSRDVARRWAYPMVPDSN 288
            +FK+H +++ RYDLIDC+IDICSPEVL LF DNFD+ D+R DFI+ +LGSELL ++LYTYV+  EYAARV+ L TY +VS+D+  RW +PMVPD+N
Sbjct:  210 LFKKHPSIQMRYDLIDCHIDICSPEVLALFIDNFDFADIREDFIHDILGSELLDHKLYTYVLQGEYAARVKDLRTYHSVSKDIIHRWTFPMVPDNN 305
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BLAST of mRNA_M-pyrifera_M_contig106162.1337.1 vs. uniprot
Match: A0A8J4UY24_9MYCE (eIF-2B GDP-GTP exchange factor subunit epsilon n=1 Tax=Polysphondylium violaceum TaxID=133409 RepID=A0A8J4UY24_9MYCE)

HSP 1 Score: 139 bits (351), Expect = 1.510e-36
Identity = 61/95 (64.21%), Postives = 78/95 (82.11%), Query Frame = 1 
Query:    4 FKEHHAVEFRYDLIDCNIDICSPEVLFLFQDNFDYKDLRRDFINGVLGSELLGYELYTYVIDKEYAARVRGLNTYAAVSRDVARRWAYPMVPDSN 288
            FK+H +V+ RYDLIDC+IDICSPEVL LF DNFD+ D+R+DFI+ +L SELL Y+L TYV+  EYAARV+ L TY +VS+D+  RW +PMVPD+N
Sbjct:  213 FKKHPSVQLRYDLIDCHIDICSPEVLALFNDNFDFTDIRKDFIHDILSSELLDYKLSTYVLQNEYAARVKDLPTYHSVSKDIIHRWTFPMVPDNN 307
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BLAST of mRNA_M-pyrifera_M_contig106162.1337.1 vs. uniprot
Match: A0A7S4IL88_9EUKA (eIF-2B GDP-GTP exchange factor subunit epsilon n=1 Tax=Vannella sp. CB-2014 TaxID=1487602 RepID=A0A7S4IL88_9EUKA)

HSP 1 Score: 138 bits (347), Expect = 5.010e-36
Identity = 58/97 (59.79%), Postives = 79/97 (81.44%), Query Frame = 1 
Query:    1 IFKEHHAVEFRYDLIDCNIDICSPEVLFLFQDNFDYKDLRRDFINGVLGSELLGYELYTYVIDKEYAARVRGLNTYAAVSRDVARRWAYPMVPDSNI 291
            +F +H+ ++ R+DL DC IDIC PEV+ LF D FD++DLR+DF+ G+L SE+L Y+++  +I++EYAARVR LNTYAAVSRD+  RW YP+VPDSNI
Sbjct:  194 LFSDHNEIQVRFDLFDCRIDICCPEVIALFHDEFDWQDLRQDFVLGILESEVLQYKIHVDIIEEEYAARVRSLNTYAAVSRDIINRWTYPLVPDSNI 290
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BLAST of mRNA_M-pyrifera_M_contig106162.1337.1 vs. uniprot
Match: A0A151ZRU9_9MYCE (eIF-2B GDP-GTP exchange factor subunit epsilon n=1 Tax=Tieghemostelium lacteum TaxID=361077 RepID=A0A151ZRU9_9MYCE)

HSP 1 Score: 135 bits (340), Expect = 5.590e-35
Identity = 59/96 (61.46%), Postives = 77/96 (80.21%), Query Frame = 1 
Query:    1 IFKEHHAVEFRYDLIDCNIDICSPEVLFLFQDNFDYKDLRRDFINGVLGSELLGYELYTYVIDKEYAARVRGLNTYAAVSRDVARRWAYPMVPDSN 288
            IF +H  V+ RYDLIDC+IDICSPEVL LF DNFD+ D+R+DFI  +L +ELL ++L+TYV+  EYAARV+ L TY +VS+D+  RW +PMVPD+N
Sbjct:  209 IFSKHQQVQLRYDLIDCHIDICSPEVLALFHDNFDFLDIRKDFIPDLLSNELLEHQLHTYVLQGEYAARVKDLRTYHSVSKDIIHRWTFPMVPDNN 304
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BLAST of mRNA_M-pyrifera_M_contig106162.1337.1 vs. uniprot
Match: EI2BE_DICDI (Translation initiation factor eIF-2B subunit epsilon n=1 Tax=Dictyostelium discoideum TaxID=44689 RepID=EI2BE_DICDI)

HSP 1 Score: 134 bits (338), Expect = 1.030e-34
Identity = 57/96 (59.38%), Postives = 78/96 (81.25%), Query Frame = 1 
Query:    1 IFKEHHAVEFRYDLIDCNIDICSPEVLFLFQDNFDYKDLRRDFINGVLGSELLGYELYTYVIDKEYAARVRGLNTYAAVSRDVARRWAYPMVPDSN 288
            +F++H +++ RYDLIDC+IDICSPEVL LF DNFD+ D+R+DFI+ +L S+LL Y+L  YV+  EYAARV+ L TY +VS+D+  RW +PMVPD+N
Sbjct:  210 LFQKHPSIQMRYDLIDCHIDICSPEVLALFNDNFDFADIRKDFIHDILTSDLLDYKLSAYVLQGEYAARVKDLRTYHSVSKDIIHRWTFPMVPDNN 305
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BLAST of mRNA_M-pyrifera_M_contig106162.1337.1 vs. uniprot
Match: D3BBS3_POLPP (eIF-2B GDP-GTP exchange factor subunit epsilon n=1 Tax=Polysphondylium pallidum (strain ATCC 26659 / Pp 5 / PN500) TaxID=670386 RepID=D3BBS3_POLPP)

HSP 1 Score: 134 bits (337), Expect = 1.420e-34
Identity = 55/96 (57.29%), Postives = 80/96 (83.33%), Query Frame = 1 
Query:    1 IFKEHHAVEFRYDLIDCNIDICSPEVLFLFQDNFDYKDLRRDFINGVLGSELLGYELYTYVIDKEYAARVRGLNTYAAVSRDVARRWAYPMVPDSN 288
            +F++H +++ RYDLIDC+IDICSPEVL LF DNFD++DLR++FI+ ++ S++L Y+L TY++  EYAARV+ L TY +VS+D+  RW +PMVPD+N
Sbjct:  207 LFQKHPSIQLRYDLIDCHIDICSPEVLALFNDNFDFEDLRKEFIHDIMQSDVLDYKLSTYILQGEYAARVKDLRTYHSVSKDIIHRWTFPMVPDNN 302
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BLAST of mRNA_M-pyrifera_M_contig106162.1337.1 vs. uniprot
Match: L8HKB0_ACACA (eIF-2B GDP-GTP exchange factor subunit epsilon (Fragment) n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8HKB0_ACACA)

HSP 1 Score: 132 bits (332), Expect = 1.960e-34
Identity = 58/96 (60.42%), Postives = 75/96 (78.12%), Query Frame = 1 
Query:    1 IFKEHHAVEFRYDLIDCNIDICSPEVLFLFQDNFDYKDLRRDFINGVLGSELLGYELYTYVIDKEYAARVRGLNTYAAVSRDVARRWAYPMVPDSN 288
            +F E+ +V+FRYDL+DC IDICSPEVL +  DNFDY DLR+DFI GV+  E+  Y+++T++I  EYAARV  L TY +VS+D+  RWAYPMVPD N
Sbjct:   37 VFAENSSVQFRYDLLDCRIDICSPEVLSVCADNFDYNDLRQDFIRGVVVDEVYKYKIFTHIISTEYAARVNILRTYDSVSKDIIHRWAYPMVPDCN 132
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BLAST of mRNA_M-pyrifera_M_contig106162.1337.1 vs. uniprot
Match: A0A0E9NEB6_SAICN (eIF-2B GDP-GTP exchange factor subunit epsilon n=2 Tax=Saitoella complicata (strain BCRC 22490 / CBS 7301 / JCM 7358 / NBRC 10748 / NRRL Y-17804) TaxID=698492 RepID=A0A0E9NEB6_SAICN)

HSP 1 Score: 131 bits (330), Expect = 1.420e-33
Identity = 56/97 (57.73%), Postives = 77/97 (79.38%), Query Frame = 1 
Query:    1 IFKEHHAVEFRYDLIDCNIDICSPEVLFLFQDNFDYKDLRRDFINGVLGSELLGYELYTYVIDKEYAARVRGLNTYAAVSRDVARRWAYPMVPDSNI 291
            IF +H  VE R DLIDC IDICSP+V  LF +NFDY+++R+DF++G+L S+LLG +++ +V +K+Y+ARVR L TY A+S+D   RW YPMVPDSN+
Sbjct:  208 IFADHPEVELRNDLIDCQIDICSPDVPLLFTENFDYQEMRQDFVHGILTSDLLGKKIHCHVAEKDYSARVRNLQTYDAISKDTLSRWTYPMVPDSNL 304
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BLAST of mRNA_M-pyrifera_M_contig106162.1337.1 vs. uniprot
Match: A0A0W4ZRQ0_PNEJ7 (eIF-2B GDP-GTP exchange factor subunit epsilon n=1 Tax=Pneumocystis jirovecii (strain RU7) TaxID=1408657 RepID=A0A0W4ZRQ0_PNEJ7)

HSP 1 Score: 130 bits (327), Expect = 3.170e-33
Identity = 55/97 (56.70%), Postives = 75/97 (77.32%), Query Frame = 1 
Query:    1 IFKEHHAVEFRYDLIDCNIDICSPEVLFLFQDNFDYKDLRRDFINGVLGSELLGYELYTYVIDKEYAARVRGLNTYAAVSRDVARRWAYPMVPDSNI 291
            IF +H  +E R DLIDCN+DICSPEV  LF +NFDY+D+RRDF++G+L S+LLG  +Y Y+ +  YAA V+ L +Y+ ++RD+  RWAYP VPDSN+
Sbjct:  210 IFNDHAKIEIRNDLIDCNVDICSPEVPALFTENFDYQDIRRDFVHGILTSDLLGKTIYCYIAEDNYAACVQSLQSYSIITRDIICRWAYPYVPDSNL 306
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BLAST of mRNA_M-pyrifera_M_contig106162.1337.1 vs. uniprot
Match: UPI000645019F (hypothetical protein n=1 Tax=Acytostelium subglobosum LB1 TaxID=1410327 RepID=UPI000645019F)

HSP 1 Score: 130 bits (327), Expect = 3.280e-33
Identity = 55/96 (57.29%), Postives = 76/96 (79.17%), Query Frame = 1 
Query:    1 IFKEHHAVEFRYDLIDCNIDICSPEVLFLFQDNFDYKDLRRDFINGVLGSELLGYELYTYVIDKEYAARVRGLNTYAAVSRDVARRWAYPMVPDSN 288
            +F +H +++ RYDLIDC+IDICS EVL LF DNFD+ DLR+DFI+  + SE+  Y+L+TY++  EYAARV+ L TY +VS+D+  RW +PMVPD+N
Sbjct:  215 LFTKHPSIQLRYDLIDCHIDICSHEVLALFSDNFDFGDLRKDFIHDCMQSEITDYKLFTYILQGEYAARVKDLRTYNSVSKDIIHRWTFPMVPDNN 310
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig106162.1337.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
F0ZYD6_DICPU2.180e-3763.54eIF-2B GDP-GTP exchange factor subunit epsilon n=1... [more]
A0A8J4UY24_9MYCE1.510e-3664.21eIF-2B GDP-GTP exchange factor subunit epsilon n=1... [more]
A0A7S4IL88_9EUKA5.010e-3659.79eIF-2B GDP-GTP exchange factor subunit epsilon n=1... [more]
A0A151ZRU9_9MYCE5.590e-3561.46eIF-2B GDP-GTP exchange factor subunit epsilon n=1... [more]
EI2BE_DICDI1.030e-3459.38Translation initiation factor eIF-2B subunit epsil... [more]
D3BBS3_POLPP1.420e-3457.29eIF-2B GDP-GTP exchange factor subunit epsilon n=1... [more]
L8HKB0_ACACA1.960e-3460.42eIF-2B GDP-GTP exchange factor subunit epsilon (Fr... [more]
A0A0E9NEB6_SAICN1.420e-3357.73eIF-2B GDP-GTP exchange factor subunit epsilon n=2... [more]
A0A0W4ZRQ0_PNEJ73.170e-3356.70eIF-2B GDP-GTP exchange factor subunit epsilon n=1... [more]
UPI000645019F3.280e-3357.29hypothetical protein n=1 Tax=Acytostelium subglobo... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig106162contigM-pyrifera_M_contig106162:4..294 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score143.7
Seed ortholog evalue4.6e-32
Seed eggNOG ortholog5786.XP_003292427.1
Preferred nameEIF2B5
KEGG koko:K03240,ko:K03859
KEGG ReactionR05916
KEGG Pathwayko00563,ko01100,ko03013,map00563,map01100,map03013
Hectar predicted targeting categoryother localisation
GOsGO:0000003,GO:0001541,GO:0002181,GO:0002183,GO:0002253,GO:0002376,GO:0002429,GO:0002682,GO:0002684,GO:0002757,GO:0002764,GO:0002768,GO:0003006,GO:0003674,GO:0003676,GO:0003723,GO:0003743,GO:0005085,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005829,GO:0005851,GO:0006412,GO:0006413,GO:0006417,GO:0006446,GO:0006518,GO:0006807,GO:0006950,GO:0007154,GO:0007165,GO:0007166,GO:0007272,GO:0007275,GO:0007399,GO:0007417,GO:0007420,GO:0007548,GO:0007568,GO:0008135,GO:0008150,GO:0008152,GO:0008366,GO:0008406,GO:0008585,GO:0009058,GO:0009059,GO:0009266,GO:0009408,GO:0009628,GO:0009719,GO:0009725,GO:0009743,GO:0009746,GO:0009749,GO:0009889,GO:0009891,GO:0009893,GO:0009987,GO:0010001,GO:0010033,GO:0010035,GO:0010038,GO:0010226,GO:0010243,GO:0010467,GO:0010468,GO:0010556,GO:0010557,GO:0010604,GO:0010608,GO:0010628,GO:0010941,GO:0010942,GO:0014002,GO:0014003,GO:0019222,GO:0019538,GO:0019899,GO:0021537,GO:0021543,GO:0021761,GO:0021766,GO:0021782,GO:0022008,GO:0022414,GO:0023052,GO:0030154,GO:0030900,GO:0031323,GO:0031325,GO:0031326,GO:0031328,GO:0031369,GO:0032045,GO:0032268,GO:0032270,GO:0032501,GO:0032502,GO:0032991,GO:0033554,GO:0034248,GO:0034250,GO:0034284,GO:0034641,GO:0034645,GO:0034976,GO:0042063,GO:0042221,GO:0042552,GO:0042981,GO:0043043,GO:0043065,GO:0043067,GO:0043068,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043434,GO:0043603,GO:0043604,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044271,GO:0044424,GO:0044444,GO:0044464,GO:0045137,GO:0045727,GO:0045948,GO:0046545,GO:0046660,GO:0048468,GO:0048513,GO:0048518,GO:0048522,GO:0048583,GO:0048584,GO:0048608,GO:0048708,GO:0048709,GO:0048731,GO:0048856,GO:0048869,GO:0050776,GO:0050778,GO:0050789,GO:0050794,GO:0050851,GO:0050852,GO:0050896,GO:0051020,GO:0051171,GO:0051173,GO:0051246,GO:0051247,GO:0051716,GO:0060255,GO:0060322,GO:0061458,GO:0065007,GO:0065009,GO:0071704,GO:0080090,GO:0097159,GO:0098772,GO:1901363,GO:1901564,GO:1901566,GO:1901576,GO:1901652,GO:1901698,GO:1901700,GO:2000112
EggNOG free text desc.eIF4-gamma/eIF5/eIF2-epsilon
EggNOG OGs3X8N3@554915,COG1208@1,KOG1461@2759
COG Functional cat.J
Best tax levelAmoebozoa
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko03012
Exons1
Model size291
Cds size291
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815571.7257726-CDS-M-pyrifera_M_contig106162:3..2941622815571.7257726-CDS-M-pyrifera_M_contig106162:3..294Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig106162 4..294 -
1692277469.10182-CDS-M-pyrifera_M_contig106162:3..2941692277469.10182-CDS-M-pyrifera_M_contig106162:3..294Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig106162 4..294 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig106162.1337.1prot_M-pyrifera_M_contig106162.1337.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig106162 4..294 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig106162.1337.1

>prot_M-pyrifera_M_contig106162.1337.1 ID=prot_M-pyrifera_M_contig106162.1337.1|Name=mRNA_M-pyrifera_M_contig106162.1337.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=97bp
IFKEHHAVEFRYDLIDCNIDICSPEVLFLFQDNFDYKDLRRDFINGVLGS
ELLGYELYTYVIDKEYAARVRGLNTYAAVSRDVARRWAYPMVPDSNI
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mRNA from alignment at M-pyrifera_M_contig106162:4..294-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig106162.1337.1 ID=mRNA_M-pyrifera_M_contig106162.1337.1|Name=mRNA_M-pyrifera_M_contig106162.1337.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=291bp|location=Sequence derived from alignment at M-pyrifera_M_contig106162:4..294- (Macrocystis pyrifera P11B4 male)
ATCTTCAAGGAGCACCACGCGGTGGAGTTCCGCTACGACCTGATTGACTG
CAACATCGACATCTGCTCACCAGAGGTGCTGTTCCTCTTCCAGGACAACT
TCGACTACAAGGACCTGCGGCGGGACTTCATCAACGGCGTGCTGGGCAGC
GAGCTTCTGGGCTACGAGCTCTACACCTACGTCATCGACAAGGAGTACGC
TGCGCGAGTGAGAGGCCTCAACACTTACGCAGCCGTGAGCAGAGACGTCG
CCAGGAGATGGGCCTATCCAATGGTGCCCGACAGCAACATC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig106162:4..294-

>mRNA_M-pyrifera_M_contig106162.1337.1 ID=mRNA_M-pyrifera_M_contig106162.1337.1|Name=mRNA_M-pyrifera_M_contig106162.1337.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=582bp|location=Sequence derived from alignment at M-pyrifera_M_contig106162:4..294- (Macrocystis pyrifera P11B4 male)
ATCTTCAAGGAGCACCACGCGGTGGAGTTCCGCTACGACCTGATTGACTG
CAACATCGACATCTGCTCACCAGAGGTGCTGTTCCTCTTCCAGGACAACT
TCGACTACAAGGACCTGCGGCGGGACTTCATCAACGGCGTGCTGGGCAGC
GAGCTTCTGGGCTACGAGCTCTACACCTACGTCATCGACAAGGAGTACGC
TGCGCGAGTGAGAGGCCTCAACACTTACGCAGCCGTGAGCAGAGACGTCG
CCAGGAGATGGGCCTATCCAATGGTGCCCGACAGCAACATCATCTTCAAG
GAGCACCACGCGGTGGAGTTCCGCTACGACCTGATTGACTGCAACATCGA
CATCTGCTCACCAGAGGTGCTGTTCCTCTTCCAGGACAACTTCGACTACA
AGGACCTGCGGCGGGACTTCATCAACGGCGTGCTGGGCAGCGAGCTTCTG
GGCTACGAGCTCTACACCTACGTCATCGACAAGGAGTACGCTGCGCGAGT
GAGAGGCCTCAACACTTACGCAGCCGTGAGCAGAGACGTCGCCAGGAGAT
GGGCCTATCCAATGGTGCCCGACAGCAACATC
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