mRNA_M-pyrifera_M_contig106135.1329.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig106135.1329.1
Unique NamemRNA_M-pyrifera_M_contig106135.1329.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig106135.1329.1 vs. uniprot
Match: R7QDH4_CHOCR (E2F_TDP domain-containing protein n=1 Tax=Chondrus crispus TaxID=2769 RepID=R7QDH4_CHOCR)

HSP 1 Score: 155 bits (393), Expect = 3.710e-43
Identity = 84/181 (46.41%), Postives = 116/181 (64.09%), Query Frame = 1
Query:   46 KPRGSRFESSLALLTQRFTALVKNHVAQPLDLNTAAQILRVQKRRIYDITNVLEGIGLLDKTTKNAIQWRGTACLNASATAQLEEVRQEVQQLKAEEERLDQQVALALGELQTDKRREDAQPLSYTIASDIYKLRCFAGKTVLVVRAPAGTTLEVPDPDDGVETGFKRFQIVLKSAGAPID 588
            K R  R++SSL LLT +F  L+K      LDLN AA+ L+VQKRRIYDITNVLEGIG+++K +KN I+WR       S  A+L E+  E++ LK E++ +DQ +A+  G+L+     E     +Y    DI  +    G T++ ++AP GT LEVPDPD+G+  G +R+QI LKS G PID
Sbjct:   65 KDRACRYDSSLGLLTTKFVNLLKASTDGALDLNLAAEKLQVQKRRIYDITNVLEGIGIIEKKSKNNIKWRHQVETGRSCEAELAELSAELESLKREDDAMDQAIAVYHGKLRALATGEQCAAFAYVTHKDIKAIPELRGDTLIAIKAPPGTELEVPDPDEGMPYGERRYQIFLKSTGGPID 245          
BLAST of mRNA_M-pyrifera_M_contig106135.1329.1 vs. uniprot
Match: A0A0M0JWF8_9EUKA (Transcription factor e2f n=1 Tax=Chrysochromulina tobinii TaxID=1460289 RepID=A0A0M0JWF8_9EUKA)

HSP 1 Score: 154 bits (389), Expect = 6.420e-43
Identity = 85/186 (45.70%), Postives = 125/186 (67.20%), Query Frame = 1
Query:   46 KPRGSRFESSLALLTQRFTALVKNHVAQPLDLNTAAQILRVQKRRIYDITNVLEGIGLLDKTTKNAIQWRGTACLNASATAQLEEVRQEVQQLKAEEERLDQQVALALGELQTDKR--REDAQPLSYTIAS--DIYKLRCFAGKTVLVVRAPAGTTLEVPDPDDGVETGFKRFQIVLKSAGAPIDV 591
            K +  R++SSL LLT++FTAL++      LDLN AAQ+L VQKRRIYDITNVLEGIG+++K +KN IQW+G    +++  A+L++++ EV+     E  LDQ    A+ E+Q+  R   ED    SY   +  D+  +  FA  TV+ ++AP+GTTLEVPDPD+G++   +R+QI L+S   P++V
Sbjct:   50 KQKTCRYDSSLGLLTKKFTALIQGAPEGVLDLNAAAQMLGVQKRRIYDITNVLEGIGIIEKRSKNNIQWKGLGANSSNMQAELDQLKGEVRSSAESETWLDQ----AIMEMQSSLRDLAEDQANASYAFVTHEDVRNINAFAADTVIAIKAPSGTTLEVPDPDEGMDYPQRRYQIYLRSTSGPVEV 231          
BLAST of mRNA_M-pyrifera_M_contig106135.1329.1 vs. uniprot
Match: L8HBK9_ACACA (Transcription factor e2f domain containing protein n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8HBK9_ACACA)

HSP 1 Score: 158 bits (399), Expect = 1.850e-42
Identity = 85/178 (47.75%), Postives = 116/178 (65.17%), Query Frame = 1
Query:   58 SRFESSLALLTQRFTALVKNHVAQPLDLNTAAQILRVQKRRIYDITNVLEGIGLLDKTTKNAIQWRGTACLNASATAQLEEVRQEVQQLKAEEERLDQQVALALGELQTDKRREDAQPLSYTIASDIYKLRCFAGKTVLVVRAPAGTTLEVPDPDDGVETGFKRFQIVLKSAGAPIDV 591
            +RF+SSL LLT++F  LV+      +DLN AA+ L VQKRRIYDITNVLEGIGL++K +KN IQWRG          +L  VR+EV QL  +E  LD+++  A   ++        Q L++    D+  L CF G T++ ++AP+GT LEVPDPD+G+    +R+QI LKS+G PIDV
Sbjct:  177 TRFDSSLGLLTKKFITLVRTAPDGSIDLNKAAEQLSVQKRRIYDITNVLEGIGLIEKKSKNHIQWRGEGIATEEERLRLGTVREEVAQLVQQELALDERIRQAQTNIKRLSDDPANQQLAFITYEDLVSLPCFQGDTLIAIKAPSGTRLEVPDPDEGMPANQRRYQIFLKSSGEPIDV 354          
BLAST of mRNA_M-pyrifera_M_contig106135.1329.1 vs. uniprot
Match: D8LEV2_ECTSI (Transcription factor E2F n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LEV2_ECTSI)

HSP 1 Score: 154 bits (388), Expect = 9.280e-42
Identity = 89/182 (48.90%), Postives = 118/182 (64.84%), Query Frame = 1
Query:   58 SRFESSLALLTQRFTALVKNHVAQPLDLNTAAQILRVQKRRIYDITNVLEGIGLLDKTTKNAIQWRGTAC-LNASATAQLEEVRQEVQQLKAEEERLDQQVALALGELQ---TDKRREDAQPLSYTIASDIYKLRCFAGKTVLVVRAPAGTTLEVPDPDDGVETGFKRFQIVLKSAGAPIDV 591
            +RF+SSL LLT+RF  L++      LDLN AA+ L VQKRRIYDITNVLEGIGL+ KT+KN IQW+G    L    T +  E ++E+ +L  +E R+DQ +A   G+L+   T+  +       +    DI  L C+   TV+ +RAPAGTTLEVPDPD+G+  G +R+QI LKS   PIDV
Sbjct:   64 TRFDSSLGLLTRRFVDLIQAAPGGTLDLNAAAKDLDVQKRRIYDITNVLEGIGLIHKTSKNHIQWKGKGDGLGEGNTEEENEAQEEIHELVDQEARVDQLIAHVKGQLKKLPTESNKPSGDMFLHQ--QDIRGLPCYKKNTVMAIRAPAGTTLEVPDPDEGMPHGERRYQIYLKSPSGPIDV 243          
BLAST of mRNA_M-pyrifera_M_contig106135.1329.1 vs. uniprot
Match: UPI001CB8CAAB (transcription factor E2FB isoform X1 n=2 Tax=Erigeron canadensis TaxID=72917 RepID=UPI001CB8CAAB)

HSP 1 Score: 154 bits (390), Expect = 1.690e-41
Identity = 84/177 (47.46%), Postives = 113/177 (63.84%), Query Frame = 1
Query:   61 RFESSLALLTQRFTALVKNHVAQPLDLNTAAQILRVQKRRIYDITNVLEGIGLLDKTTKNAIQWRGTACLNASATAQLEEVRQEVQQLKAEEERLDQQVALALGELQTDKRREDAQPLSYTIASDIYKLRCFAGKTVLVVRAPAGTTLEVPDPDDGVETGFKRFQIVLKSAGAPIDV 591
            R++SSL LLT++F  L+K+     LDLN AA+ L VQKRRIYDITNVLEGIGL++K  KN IQW+G    N      +  ++ EV+ L AEE RLD+Q       L+     E+ Q   +    DI  L CF  +T++ ++AP GTTLEVPDPD+ V+   +R++IVL+S   PIDV
Sbjct:  132 RYDSSLGLLTKKFINLIKHAEDGMLDLNKAAETLEVQKRRIYDITNVLEGIGLIEKKLKNRIQWKGLDASNGEVDGNVTSLQAEVENLLAEELRLDEQTREMQERLRDLSEDENNQKFLFVTEEDIKNLPCFRDETLIAIKAPHGTTLEVPDPDEAVDYPQRRYRIVLRSTMGPIDV 308          
BLAST of mRNA_M-pyrifera_M_contig106135.1329.1 vs. uniprot
Match: D8SK88_SELML (Uncharacterized protein E2F1-1 n=7 Tax=Selaginella moellendorffii TaxID=88036 RepID=D8SK88_SELML)

HSP 1 Score: 151 bits (381), Expect = 2.570e-41
Identity = 85/183 (46.45%), Postives = 116/183 (63.39%), Query Frame = 1
Query:   49 PRGSRFESSLALLTQRFTALVKNHVAQPLDLNTAAQILRVQKRRIYDITNVLEGIGLLDKTTKNAIQWRGT--ACLNASATAQLEEVRQEVQQLKAEEERLDQQVALALGELQTDKRREDAQPLSYTIASDIYKLRCFAGKTVLVVRAPAGTTLEVPDPDDGVETGFKRFQIVLKSAGAPIDV 591
            P   R++SSL LLT++F  L+       LDLN AA  L VQKRRIYDITNVLEGIGL++K  KN I+W+G   +  ++ +T  L  ++ EV  L A+EE LD+++     +L+T       Q   Y    DI  L CF  +T++ ++AP GTTLEVPDPD+ VE   +R+QI+L+SA  PIDV
Sbjct:   35 PSTCRYDSSLGLLTKKFINLIDQAEDGVLDLNNAADTLHVQKRRIYDITNVLEGIGLIEKKLKNRIRWKGLGLSVADSDSTGLLSSLQSEVDDLHAQEESLDEKIRAMREKLKTMCEDVSNQQWLYVTEDDIKGLPCFQNETLIAIKAPQGTTLEVPDPDEAVEYPQRRYQILLRSAFGPIDV 217          
BLAST of mRNA_M-pyrifera_M_contig106135.1329.1 vs. uniprot
Match: U5D432_AMBTC (E2F_TDP domain-containing protein n=2 Tax=Amborella trichopoda TaxID=13333 RepID=U5D432_AMBTC)

HSP 1 Score: 153 bits (386), Expect = 4.090e-41
Identity = 87/181 (48.07%), Postives = 118/181 (65.19%), Query Frame = 1
Query:   61 RFESSLALLTQRFTALVKNHVAQPLDLNTAAQILRVQKRRIYDITNVLEGIGLLDKTTKNAIQWRGTACLNASATAQLEE----VRQEVQQLKAEEERLDQQVALALGELQTDKRREDAQPLSYTIASDIYKLRCFAGKTVLVVRAPAGTTLEVPDPDDGVETGFKRFQIVLKSAGAPIDV 591
            R++SSL LLT++F  L+K      LDLN AA+IL VQKRRIYDITNVLEGIGL++K  KN I+W+G   L+ S   +++E    ++ EVQ L  EE R+D+Q+      L+     E+ Q   Y    DI  L CF  +T++ ++AP GTTLEVPDPD+ VE   +R+QI+L+S   PIDV
Sbjct:  138 RYDSSLGLLTKKFINLIKQAEDGVLDLNKAAEILEVQKRRIYDITNVLEGIGLIEKKLKNRIRWKG---LDVSRPGEVDEDIAVLQAEVQNLHLEEHRIDEQIREMQKSLRDLSEDENNQKWLYVTEEDIKGLPCFQNETLIAIKAPHGTTLEVPDPDEAVEYPQRRYQILLRSTMGPIDV 315          
BLAST of mRNA_M-pyrifera_M_contig106135.1329.1 vs. uniprot
Match: A0A5J4Y413_9CHLO (Transcription factor E2FB-like n=1 Tax=Trebouxia sp. A1-2 TaxID=2608996 RepID=A0A5J4Y413_9CHLO)

HSP 1 Score: 150 bits (378), Expect = 4.440e-41
Identity = 94/182 (51.65%), Postives = 118/182 (64.84%), Query Frame = 1
Query:   61 RFESSLALLTQRFTALVKNHVAQPLDLNTAAQILRVQKRRIYDITNVLEGIGLLDKTTKNAIQWRGTACLNASATAQLEE---VRQEVQQLKAEEERLDQQVALALGELQTDKRREDAQPLS-YTIASDIYKLRCFAGKTVLVVRAPAGTTLEVPDPDDGVETGFKRFQIVLKS-AGAPIDV 591
            R++SSL LLT++F  LV+      LDLN AA  L VQKRRIYDITNVLEGIGL++K  KN IQW+G +  +A AT  L E   +RQE+  L+ +E+ L + +  AL +   D   + A     Y    DI KL C A  TV  V+AP GTTLEVPDPDDG+ETG +R++IVLKS    PIDV
Sbjct:   32 RYDSSLGLLTKKFVNLVEAAPDGVLDLNKAADALAVQKRRIYDITNVLEGIGLIEKKLKNNIQWKGGS--SADATDNLPEQAALRQEISNLQEDEQNLKRHIT-ALEDSIKDMTEDAANSARLYVTDEDIAKLPCTANDTVFAVKAPQGTTLEVPDPDDGLETGQRRYRIVLKSNCDTPIDV 210          
BLAST of mRNA_M-pyrifera_M_contig106135.1329.1 vs. uniprot
Match: A0A484EDS3_BRELC (E2F_TDP domain-containing protein n=1 Tax=Bremia lactucae TaxID=4779 RepID=A0A484EDS3_BRELC)

HSP 1 Score: 151 bits (381), Expect = 4.550e-41
Identity = 84/186 (45.16%), Postives = 120/186 (64.52%), Query Frame = 1
Query:   58 SRFESSLALLTQRFTALVKNHVAQPLDLNTAAQILRVQKRRIYDITNVLEGIGLLDKTTKNAIQWRGTACLNASATAQ--LEEVRQEVQQLKAEEERLDQQVALALGELQTDKRREDA------QPLSYTIASDIYKLRCFAGKTVLVVRAPAGTTLEVPDPDDGVETGFKRFQIVLKSAGAPIDV 591
            SR+++SL LLT+RF  L++   ++ LDLNTAA+ L VQKRRIYDITNVLEGIGL++KT+KN I W+G +  + S      ++ +RQ +  L+ EE + DQ +      ++     EDA      +   Y    D+ +   FA ++V+ ++AP GTTLEVPDPD+G+E G +RFQI LKS   P+DV
Sbjct:   66 SRYDNSLGLLTKRFVELIQAVPSKDLDLNTAAESLGVQKRRIYDITNVLEGIGLIEKTSKNNIHWKGASGRSGSTDTYQGMDHLRQNISDLRQEELKYDQHIKTVSQSIR-QLYEEDAFDKGSFENFCYVTHDDMRRQESFADQSVMAIKAPPGTTLEVPDPDEGMEAGERRFQIFLKSTEGPVDV 250          
BLAST of mRNA_M-pyrifera_M_contig106135.1329.1 vs. uniprot
Match: A0A5A7P3J8_STRAF (Transcription factor n=1 Tax=Striga asiatica TaxID=4170 RepID=A0A5A7P3J8_STRAF)

HSP 1 Score: 152 bits (385), Expect = 9.410e-41
Identity = 85/179 (47.49%), Postives = 120/179 (67.04%), Query Frame = 1
Query:   61 RFESSLALLTQRFTALVKNHVAQPLDLNTAAQILRVQKRRIYDITNVLEGIGLLDKTTKNAIQWRGTACLN-ASATAQLEEVRQEVQQLKAEEERLDQQVALALGELQTD-KRREDAQPLSYTIASDIYKLRCFAGKTVLVVRAPAGTTLEVPDPDDGVETGFKRFQIVLKSAGAPIDV 591
            R++SSL+LLT++FT L+K+     +DLN AA  L VQKRRIYDITNVLEGIGL++K  KN IQW+G      A A   +  +R+E+Q LK EE+ LD+++   + E+ TD    E+ Q   +    DI  L CF  +T++ ++AP GTTLEVPDPD+ V+   +R++I+L+S   PIDV
Sbjct:  124 RYDSSLSLLTKKFTDLIKHAEDGIIDLNNAADTLEVQKRRIYDITNVLEGIGLIEKKLKNRIQWKGADVSRPAQAQGNISGLRKEIQNLKMEEQGLDKRIR-EMQEILTDLSEDENNQRWLFVTEEDIKNLPCFQNQTLIAIKAPHGTTLEVPDPDETVDYSQRRYRIILRSTMGPIDV 301          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig106135.1329.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
R7QDH4_CHOCR3.710e-4346.41E2F_TDP domain-containing protein n=1 Tax=Chondrus... [more]
A0A0M0JWF8_9EUKA6.420e-4345.70Transcription factor e2f n=1 Tax=Chrysochromulina ... [more]
L8HBK9_ACACA1.850e-4247.75Transcription factor e2f domain containing protein... [more]
D8LEV2_ECTSI9.280e-4248.90Transcription factor E2F n=1 Tax=Ectocarpus silicu... [more]
UPI001CB8CAAB1.690e-4147.46transcription factor E2FB isoform X1 n=2 Tax=Erige... [more]
D8SK88_SELML2.570e-4146.45Uncharacterized protein E2F1-1 n=7 Tax=Selaginella... [more]
U5D432_AMBTC4.090e-4148.07E2F_TDP domain-containing protein n=2 Tax=Amborell... [more]
A0A5J4Y413_9CHLO4.440e-4151.65Transcription factor E2FB-like n=1 Tax=Trebouxia s... [more]
A0A484EDS3_BRELC4.550e-4145.16E2F_TDP domain-containing protein n=1 Tax=Bremia l... [more]
A0A5A7P3J8_STRAF9.410e-4147.49Transcription factor n=1 Tax=Striga asiatica TaxID... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig106135contigM-pyrifera_M_contig106135:2..592 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score154.1
Seed ortholog evalue6.9e-35
Seed eggNOG ortholog2880.D8LEV2
KEGG koko:K04682,ko:K06620
KEGG Pathwayko01522,ko04110,ko04218,ko04350,ko04934,ko05161,ko05166,ko05167,ko05200,ko05206,ko05212,ko05214,ko05215,ko05218,ko05219,ko05220,ko05222,ko05223,ko05224,ko05225,ko05226,map01522,map04110,map04218,map04350,map04934,map05161,map05166,map05167,map05200,map05206,map05212,map05214,map05215,map05218,map05219,map05220,map05222,map05223,map05224,map05225,map05226
KEGG ModuleM00692
Hectar predicted targeting categoryother localisation
EggNOG free text desc.protein dimerization activity
EggNOG OGsKOG2577@1,KOG2577@2759
COG Functional cat.K
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko03000
Exons1
Model size591
Cds size591
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815571.3201294-CDS-M-pyrifera_M_contig106135:1..5921622815571.3201294-CDS-M-pyrifera_M_contig106135:1..592Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig106135 2..592 -
1692277468.8927262-CDS-M-pyrifera_M_contig106135:1..5921692277468.8927262-CDS-M-pyrifera_M_contig106135:1..592Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig106135 2..592 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig106135.1329.1prot_M-pyrifera_M_contig106135.1329.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig106135 2..592 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig106135.1329.1

>prot_M-pyrifera_M_contig106135.1329.1 ID=prot_M-pyrifera_M_contig106135.1329.1|Name=mRNA_M-pyrifera_M_contig106135.1329.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=197bp
PRGAAGSGKAAPAGRKPRGSRFESSLALLTQRFTALVKNHVAQPLDLNTA
AQILRVQKRRIYDITNVLEGIGLLDKTTKNAIQWRGTACLNASATAQLEE
VRQEVQQLKAEEERLDQQVALALGELQTDKRREDAQPLSYTIASDIYKLR
CFAGKTVLVVRAPAGTTLEVPDPDDGVETGFKRFQIVLKSAGAPIDV
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mRNA from alignment at M-pyrifera_M_contig106135:2..592-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig106135.1329.1 ID=mRNA_M-pyrifera_M_contig106135.1329.1|Name=mRNA_M-pyrifera_M_contig106135.1329.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=591bp|location=Sequence derived from alignment at M-pyrifera_M_contig106135:2..592- (Macrocystis pyrifera P11B4 male)
CCAAGAGGCGCAGCAGGTTCAGGCAAGGCGGCGCCGGCGGGCCGCAAGCC CCGCGGGTCGCGCTTCGAGAGCTCCCTGGCACTGTTGACGCAGCGGTTCA CGGCGTTGGTGAAGAACCACGTTGCGCAGCCGCTGGACCTGAACACTGCG GCTCAAATCCTGCGCGTGCAGAAGCGACGCATCTACGACATCACCAACGT GCTCGAAGGCATCGGCCTGCTGGACAAAACCACCAAGAACGCCATCCAGT GGCGCGGCACCGCGTGCTTGAACGCGTCCGCCACCGCGCAGCTGGAGGAG GTGCGGCAGGAGGTGCAGCAGCTCAAGGCGGAGGAGGAGCGGCTTGACCA GCAGGTGGCGCTGGCGCTGGGCGAGCTGCAGACGGACAAGCGGCGGGAAG ACGCGCAGCCGCTCTCCTACACCATCGCTTCCGACATCTACAAGCTGCGC TGCTTCGCGGGGAAGACCGTACTCGTCGTACGCGCGCCGGCTGGCACCAC GCTGGAGGTGCCGGACCCGGACGACGGCGTGGAAACCGGCTTCAAGCGCT TCCAGATCGTACTCAAGAGCGCGGGCGCGCCCATCGACGTG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig106135:2..592-

>mRNA_M-pyrifera_M_contig106135.1329.1 ID=mRNA_M-pyrifera_M_contig106135.1329.1|Name=mRNA_M-pyrifera_M_contig106135.1329.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1182bp|location=Sequence derived from alignment at M-pyrifera_M_contig106135:2..592- (Macrocystis pyrifera P11B4 male)
CCAAGAGGCGCAGCAGGTTCAGGCAAGGCGGCGCCGGCGGGCCGCAAGCC
CCGCGGGTCGCGCTTCGAGAGCTCCCTGGCACTGTTGACGCAGCGGTTCA
CGGCGTTGGTGAAGAACCACGTTGCGCAGCCGCTGGACCTGAACACTGCG
GCTCAAATCCTGCGCGTGCAGAAGCGACGCATCTACGACATCACCAACGT
GCTCGAAGGCATCGGCCTGCTGGACAAAACCACCAAGAACGCCATCCAGT
GGCGCGGCACCGCGTGCTTGAACGCGTCCGCCACCGCGCAGCTGGAGGAG
GTGCGGCAGGAGGTGCAGCAGCTCAAGGCGGAGGAGGAGCGGCTTGACCA
GCAGGTGGCGCTGGCGCTGGGCGAGCTGCAGACGGACAAGCGGCGGGAAG
ACGCGCAGCCGCTCTCCTACACCATCGCTTCCGACATCTACAAGCTGCGC
TGCTTCGCGGGGAAGACCGTACTCGTCGTACGCGCGCCGGCTGGCACCAC
GCTGGAGGTGCCGGACCCGGACGACGGCGTGGAAACCGGCTTCAAGCGCT
TCCAGATCGTACTCAAGAGCGCGGGCGCGCCCATCGACGTGCCAAGAGGC
GCAGCAGGTTCAGGCAAGGCGGCGCCGGCGGGCCGCAAGCCCCGCGGGTC
GCGCTTCGAGAGCTCCCTGGCACTGTTGACGCAGCGGTTCACGGCGTTGG
TGAAGAACCACGTTGCGCAGCCGCTGGACCTGAACACTGCGGCTCAAATC
CTGCGCGTGCAGAAGCGACGCATCTACGACATCACCAACGTGCTCGAAGG
CATCGGCCTGCTGGACAAAACCACCAAGAACGCCATCCAGTGGCGCGGCA
CCGCGTGCTTGAACGCGTCCGCCACCGCGCAGCTGGAGGAGGTGCGGCAG
GAGGTGCAGCAGCTCAAGGCGGAGGAGGAGCGGCTTGACCAGCAGGTGGC
GCTGGCGCTGGGCGAGCTGCAGACGGACAAGCGGCGGGAAGACGCGCAGC
CGCTCTCCTACACCATCGCTTCCGACATCTACAAGCTGCGCTGCTTCGCG
GGGAAGACCGTACTCGTCGTACGCGCGCCGGCTGGCACCACGCTGGAGGT
GCCGGACCCGGACGACGGCGTGGAAACCGGCTTCAAGCGCTTCCAGATCG
TACTCAAGAGCGCGGGCGCGCCCATCGACGTG
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