mRNA_M-pyrifera_M_contig106002.1284.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig106002.1284.1
Unique NamemRNA_M-pyrifera_M_contig106002.1284.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig106002.1284.1 vs. uniprot
Match: A0A1B6Y8V7_9BACT (tRNA modification GTPase MnmE n=8 Tax=Bacteroidetes/Chlorobi group TaxID=68336 RepID=A0A1B6Y8V7_9BACT)

HSP 1 Score: 263 bits (671), Expect = 6.880e-84
Identity = 132/157 (84.08%), Postives = 148/157 (94.27%), Query Frame = 1 
Query:    1 QADLVIYLKDLSSPFSDEERKEIAEFQQRAGETPFILIGTKADIEADKEWRTEFDVKISALKGDQIQSLKKLLKNRALENKHYDASSILVTSSRHRDALQKTQEHIQSALIGLARGMTGDFLSIDLRAALNELGTVTGEITNEHVLDSIFSRFCIGK 471
            QADLVIYLKDLS  F+++ERKEIA FQ+RAGETPFILIGTKADIEAD+EWRTEFD+KISA++ + I++LK LLKNRALENKHYDASS+LVTSSRHRDALQKT+EHIQ+AL GL  G+TGDFLSIDLRAALNELGTVTGEITNEH+LDSIFSRFCIGK
Sbjct:  307 QADLVIYLKDLSQAFTEDERKEIAAFQKRAGETPFILIGTKADIEADREWRTEFDLKISAVEEENIKALKTLLKNRALENKHYDASSLLVTSSRHRDALQKTKEHIQAALTGLENGLTGDFLSIDLRAALNELGTVTGEITNEHILDSIFSRFCIGK 463
[Close]
BLAST of mRNA_M-pyrifera_M_contig106002.1284.1 vs. uniprot
Match: UPI00035F99E6 (tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE n=1 Tax=Balneola vulgaris TaxID=287535 RepID=UPI00035F99E6)

HSP 1 Score: 259 bits (663), Expect = 9.330e-83
Identity = 129/157 (82.17%), Postives = 147/157 (93.63%), Query Frame = 1 
Query:    1 QADLVIYLKDLSSPFSDEERKEIAEFQQRAGETPFILIGTKADIEADKEWRTEFDVKISALKGDQIQSLKKLLKNRALENKHYDASSILVTSSRHRDALQKTQEHIQSALIGLARGMTGDFLSIDLRAALNELGTVTGEITNEHVLDSIFSRFCIGK 471
            QADLV+YLKDLS PF+D+ERKEIA+FQ+RAGETPFIL+GTK D E D+EWRTEFD+KISA+ G++I+ LK+LLK+RALENKHYDASS+LVTS+RHRDALQKTQ HIQ+AL GL  GMTGDFLSIDLRAALNELGTVTGEITNEH+LDSIFSRFCIGK
Sbjct:  301 QADLVVYLKDLSHPFTDDERKEIADFQKRAGETPFILVGTKKDQEIDEEWRTEFDLKISAIDGEKIKELKQLLKDRALENKHYDASSLLVTSTRHRDALQKTQSHIQAALNGLDLGMTGDFLSIDLRAALNELGTVTGEITNEHILDSIFSRFCIGK 457
[Close]
BLAST of mRNA_M-pyrifera_M_contig106002.1284.1 vs. uniprot
Match: A0A6A8Q721_9BACT (tRNA modification GTPase MnmE n=1 Tax=Balneolaceae bacterium TaxID=2053516 RepID=A0A6A8Q721_9BACT)

HSP 1 Score: 254 bits (649), Expect = 1.220e-80
Identity = 128/157 (81.53%), Postives = 145/157 (92.36%), Query Frame = 1 
Query:    1 QADLVIYLKDLSSPFSDEERKEIAEFQQRAGETPFILIGTKADIEADKEWRTEFDVKISALKGDQIQSLKKLLKNRALENKHYDASSILVTSSRHRDALQKTQEHIQSALIGLARGMTGDFLSIDLRAALNELGTVTGEITNEHVLDSIFSRFCIGK 471
            +A+LVIYLKDLS PF +EERKEIA FQQ++GETPFILIGTKADIEADKEWRTEFD+KISA++G++I  LK+LLK+RALENKHYDASS+LVTSSRHRDALQKTQEH+Q+AL GL   MTGDFLSIDLRAAL ELGT+TGEIT E +LDSIFSRFCIGK
Sbjct:  302 RANLVIYLKDLSKPFDEEERKEIAAFQQKSGETPFILIGTKADIEADKEWRTEFDLKISAVEGEKINDLKQLLKDRALENKHYDASSLLVTSSRHRDALQKTQEHVQAALKGLNMNMTGDFLSIDLRAALKELGTITGEITTEDLLDSIFSRFCIGK 458
[Close]
BLAST of mRNA_M-pyrifera_M_contig106002.1284.1 vs. uniprot
Match: A0A2D8CJ25_9BACT (tRNA modification GTPase MnmE n=5 Tax=Balneolaceae TaxID=1813606 RepID=A0A2D8CJ25_9BACT)

HSP 1 Score: 254 bits (648), Expect = 1.690e-80
Identity = 128/157 (81.53%), Postives = 148/157 (94.27%), Query Frame = 1 
Query:    1 QADLVIYLKDLSSPFSDEERKEIAEFQQRAGETPFILIGTKADIEADKEWRTEFDVKISALKGDQIQSLKKLLKNRALENKHYDASSILVTSSRHRDALQKTQEHIQSALIGLARGMTGDFLSIDLRAALNELGTVTGEITNEHVLDSIFSRFCIGK 471
            QADLVIYLKDLS  F+++ERKEIA+FQ+R+ ETPFILIGTKADIEA+KEWRTEFD+KISAL+G++I+ LK+LLK+RALENKHYDASS+LVTS+RHRDALQKT+EH+QSAL  LA+ MTGDFLSIDLRAAL ELGT+TGEITNE VLDSIFSRFCIGK
Sbjct:  301 QADLVIYLKDLSQAFTEDERKEIADFQKRSKETPFILIGTKADIEAEKEWRTEFDLKISALEGEKIKELKQLLKDRALENKHYDASSLLVTSTRHRDALQKTKEHVQSALRALAQDMTGDFLSIDLRAALKELGTITGEITNEDVLDSIFSRFCIGK 457
[Close]
BLAST of mRNA_M-pyrifera_M_contig106002.1284.1 vs. uniprot
Match: A0A3M8G1F7_9BACT (tRNA modification GTPase MnmE n=1 Tax=Balneola sp. TaxID=2024824 RepID=A0A3M8G1F7_9BACT)

HSP 1 Score: 238 bits (607), Expect = 2.500e-74
Identity = 119/156 (76.28%), Postives = 138/156 (88.46%), Query Frame = 1 
Query:    4 ADLVIYLKDLSSPFSDEERKEIAEFQQRAGETPFILIGTKADIEADKEWRTEFDVKISALKGDQIQSLKKLLKNRALENKHYDASSILVTSSRHRDALQKTQEHIQSALIGLARGMTGDFLSIDLRAALNELGTVTGEITNEHVLDSIFSRFCIGK 471
            ADL IYLK+L++PF + ER+EIA  QQ+AGETPFIL+GTKADIE ++E R  FD+KISA  GD I  LKKLLKNRALENKHYDASSILVTS+RHRD LQK ++H+Q+ALIGL + +TGDFLSIDLRAALNELGT+TGEITNEH+LDSIFSRFCIGK
Sbjct:  303 ADLAIYLKELNTPFDEVERREIAALQQKAGETPFILLGTKADIEIERESRIVFDLKISAANGDGIDRLKKLLKNRALENKHYDASSILVTSTRHRDGLQKAKDHVQAALIGLNQNLTGDFLSIDLRAALNELGTITGEITNEHILDSIFSRFCIGK 458
[Close]
BLAST of mRNA_M-pyrifera_M_contig106002.1284.1 vs. uniprot
Match: A0A6M1SXM4_9BACT (tRNA modification GTPase MnmE n=1 Tax=Balneolaceae bacterium YR4-1 TaxID=2709311 RepID=A0A6M1SXM4_9BACT)

HSP 1 Score: 231 bits (590), Expect = 9.560e-72
Identity = 116/158 (73.42%), Postives = 140/158 (88.61%), Query Frame = 1 
Query:    1 QADLVIYLKDLSSPFSDEERKEIAEFQQRAGETPFILIGTKADIEADKEW-RTEFDVKISALKGDQIQSLKKLLKNRALENKHYDASSILVTSSRHRDALQKTQEHIQSALIGLARGMTGDFLSIDLRAALNELGTVTGEITNEHVLDSIFSRFCIGK 471
            +ADL +YLKDLS PF+ EER+++  FQ++AG+TPF+L+GTK DIE +KE  R ++D+KISAL+G++I+ LKKL+K RALENKHYDASS+LVTSSRHRDAL K QEH++SAL  L RGMTGDFLSIDLRAALNELGT+TGEITNE VLDSIFSRFCIGK
Sbjct:  304 EADLAVYLKDLSKPFAPEEREDLGGFQKKAGDTPFVLVGTKKDIELEKEEERLDYDLKISALEGEKIEELKKLMKQRALENKHYDASSLLVTSSRHRDALLKAQEHVRSALQALDRGMTGDFLSIDLRAALNELGTITGEITNEDVLDSIFSRFCIGK 461
[Close]
BLAST of mRNA_M-pyrifera_M_contig106002.1284.1 vs. uniprot
Match: UPI00110992DA (tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE n=1 Tax=Aliifodinibius saliphilus TaxID=1920650 RepID=UPI00110992DA)

HSP 1 Score: 215 bits (547), Expect = 3.230e-65
Identity = 112/158 (70.89%), Postives = 133/158 (84.18%), Query Frame = 1 
Query:    1 QADLVIYLKDLSSPFSDEERKEIAEFQQRAGETPFILIGTKADIEAD-KEWRTEFDVKISALKGDQIQSLKKLLKNRALENKHYDASSILVTSSRHRDALQKTQEHIQSALIGLARGMTGDFLSIDLRAALNELGTVTGEITNEHVLDSIFSRFCIGK 471
            +ADLV+YLKDLSS  + EER+EIA+ Q+ AGETPFILIGTK DI  D  + R ++D+KISAL+G+ I  LKKL+K RALENKHYD+SS+LVTSSRHRDAL+K Q+ +QSAL  L RGMTGDFLSIDLR+AL  LGT+TGEIT E +LDSIFSRFCIGK
Sbjct:  313 KADLVVYLKDLSSEITIEEREEIAKLQKNAGETPFILIGTKKDIALDIDDERIDYDLKISALEGEAIDKLKKLMKRRALENKHYDSSSLLVTSSRHRDALEKAQQDVQSALQALDRGMTGDFLSIDLRSALKHLGTITGEITTEDILDSIFSRFCIGK 470
[Close]
BLAST of mRNA_M-pyrifera_M_contig106002.1284.1 vs. uniprot
Match: A0A316TRF8_9BACT (tRNA modification GTPase MnmE n=1 Tax=Rhodohalobacter mucosus TaxID=2079485 RepID=A0A316TRF8_9BACT)

HSP 1 Score: 213 bits (542), Expect = 1.450e-64
Identity = 110/160 (68.75%), Postives = 131/160 (81.88%), Query Frame = 1 
Query:    1 QADLVIYLKDLSSPFSDEERKEIAEFQQRAGETPFILIGTKADIEA---DKEWRTEFDVKISALKGDQIQSLKKLLKNRALENKHYDASSILVTSSRHRDALQKTQEHIQSALIGLARGMTGDFLSIDLRAALNELGTVTGEITNEHVLDSIFSRFCIGK 471
            QADL++YLKDLS P    ER+EIA FQ+RAGETPF+L+GTK D+ A   DK+ R  FD+KISA+K + I +LKKL+K RALENK YD SS+LVTSSRHRDALQK +EHI+ A+  L  GMTGDFLSIDLR++L +LG +TGEITNE VLDSIFSRFCIGK
Sbjct:  302 QADLIVYLKDLSQPLHTSEREEIAGFQKRAGETPFLLVGTKLDLAAGRDDKDERIHFDLKISAIKEENIDTLKKLMKQRALENKDYDTSSLLVTSSRHRDALQKAREHIRRAVEALDSGMTGDFLSIDLRSSLRQLGEITGEITNEDVLDSIFSRFCIGK 461
[Close]
BLAST of mRNA_M-pyrifera_M_contig106002.1284.1 vs. uniprot
Match: A0A2N0VEY6_9BACT (tRNA modification GTPase MnmE n=3 Tax=Rhodohalobacter TaxID=1974209 RepID=A0A2N0VEY6_9BACT)

HSP 1 Score: 213 bits (541), Expect = 1.770e-64
Identity = 110/158 (69.62%), Postives = 130/158 (82.28%), Query Frame = 1 
Query:    1 QADLVIYLKDLSSPFSDEERKEIAEFQQRAGETPFILIGTKADIE-ADKEWRTEFDVKISALKGDQIQSLKKLLKNRALENKHYDASSILVTSSRHRDALQKTQEHIQSALIGLARGMTGDFLSIDLRAALNELGTVTGEITNEHVLDSIFSRFCIGK 471
            QADLV+YLKDLS P   EER+E+A FQ+RA  TPF+LIGTK D+E   +E R ++D+KISAL+   I  LKKL+K RALENK YD SS+LVTSSRHRDALQK +E++Q A+  L +GMTGDFLSIDLR+AL ELG +TGEITNEHVLDSIFSRFCIGK
Sbjct:  298 QADLVVYLKDLSLPLDPEEREEVAGFQKRAAATPFLLIGTKLDLEDTSEEERIDYDLKISALEEQNIDDLKKLMKQRALENKDYDTSSLLVTSSRHRDALQKARENVQRAIQALDQGMTGDFLSIDLRSALKELGAITGEITNEHVLDSIFSRFCIGK 455
[Close]
BLAST of mRNA_M-pyrifera_M_contig106002.1284.1 vs. uniprot
Match: A0A6I7QDX7_9BACT (tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE (Fragment) n=1 Tax=Balneolaceae bacterium TaxID=2053516 RepID=A0A6I7QDX7_9BACT)

HSP 1 Score: 211 bits (538), Expect = 5.220e-64
Identity = 111/167 (66.47%), Postives = 130/167 (77.84%), Query Frame = 1 
Query:    1 QADLVIYLKDLSSPFSDEERKEIAEFQQRAGETPFILIGTKADIEAD----------KEWRTEFDVKISALKGDQIQSLKKLLKNRALENKHYDASSILVTSSRHRDALQKTQEHIQSALIGLARGMTGDFLSIDLRAALNELGTVTGEITNEHVLDSIFSRFCIGK 471
            QADLV+YLKDLS P   EER+EIA FQ+RA +TPF+LIGTK D+             KE R  +D+KISA++   I  LKKL+K+RALENK YD SS+LVTSSRHRD LQK +E++Q A+  L RGMTGDFLSIDLRAAL ELGT+TGE+TNEHVLDSIFSRFCIGK
Sbjct:  291 QADLVVYLKDLSLPLDTEEREEIAGFQKRAAQTPFLLIGTKLDLNQSTPDSLSGGGGKEERIHYDLKISAIEEQNIDELKKLMKHRALENKDYDTSSLLVTSSRHRDGLQKARENVQRAIDALDRGMTGDFLSIDLRAALKELGTITGEVTNEHVLDSIFSRFCIGK 457
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig106002.1284.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A1B6Y8V7_9BACT6.880e-8484.08tRNA modification GTPase MnmE n=8 Tax=Bacteroidete... [more]
UPI00035F99E69.330e-8382.17tRNA uridine-5-carboxymethylaminomethyl(34) synthe... [more]
A0A6A8Q721_9BACT1.220e-8081.53tRNA modification GTPase MnmE n=1 Tax=Balneolaceae... [more]
A0A2D8CJ25_9BACT1.690e-8081.53tRNA modification GTPase MnmE n=5 Tax=Balneolaceae... [more]
A0A3M8G1F7_9BACT2.500e-7476.28tRNA modification GTPase MnmE n=1 Tax=Balneola sp.... [more]
A0A6M1SXM4_9BACT9.560e-7273.42tRNA modification GTPase MnmE n=1 Tax=Balneolaceae... [more]
UPI00110992DA3.230e-6570.89tRNA uridine-5-carboxymethylaminomethyl(34) synthe... [more]
A0A316TRF8_9BACT1.450e-6468.75tRNA modification GTPase MnmE n=1 Tax=Rhodohalobac... [more]
A0A2N0VEY6_9BACT1.770e-6469.62tRNA modification GTPase MnmE n=3 Tax=Rhodohalobac... [more]
A0A6I7QDX7_9BACT5.220e-6466.47tRNA uridine-5-carboxymethylaminomethyl(34) synthe... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig106002contigM-pyrifera_M_contig106002:3..476 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score264.2
Seed ortholog evalue3.8e-68
Seed eggNOG ortholog1121104.AQXH01000001_gene2077
Preferred namemnmE
KEGG rclassRC00053,RC00209,RC00870
KEGG koko:K03650
KEGG ReactionR08701
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0005488,GO:0005515,GO:0042802
EggNOG free text desc.Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34
EggNOG OGs1IPFD@117747,4NECT@976,COG0486@1,COG0486@2
COG Functional cat.S
Best tax levelSphingobacteriia
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko03016
Exons1
Model size474
Cds size474
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815569.2958586-CDS-M-pyrifera_M_contig106002:2..4761622815569.2958586-CDS-M-pyrifera_M_contig106002:2..476Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig106002 3..476 +
1692277468.124408-CDS-M-pyrifera_M_contig106002:2..4761692277468.124408-CDS-M-pyrifera_M_contig106002:2..476Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig106002 3..476 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig106002.1284.1prot_M-pyrifera_M_contig106002.1284.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig106002 3..476 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig106002.1284.1

>prot_M-pyrifera_M_contig106002.1284.1 ID=prot_M-pyrifera_M_contig106002.1284.1|Name=mRNA_M-pyrifera_M_contig106002.1284.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=158bp
QADLVIYLKDLSSPFSDEERKEIAEFQQRAGETPFILIGTKADIEADKEW
RTEFDVKISALKGDQIQSLKKLLKNRALENKHYDASSILVTSSRHRDALQ
KTQEHIQSALIGLARGMTGDFLSIDLRAALNELGTVTGEITNEHVLDSIF
SRFCIGK*
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mRNA from alignment at M-pyrifera_M_contig106002:3..476+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig106002.1284.1 ID=mRNA_M-pyrifera_M_contig106002.1284.1|Name=mRNA_M-pyrifera_M_contig106002.1284.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=474bp|location=Sequence derived from alignment at M-pyrifera_M_contig106002:3..476+ (Macrocystis pyrifera P11B4 male)
CAAGCCGACCTTGTTATTTACTTAAAGGATTTATCAAGTCCATTTAGTGA
TGAAGAACGAAAAGAAATTGCCGAATTTCAGCAACGAGCTGGTGAAACCC
CATTTATTTTGATTGGAACCAAAGCAGATATCGAAGCCGATAAAGAATGG
CGGACAGAGTTTGATGTAAAAATATCTGCATTGAAAGGGGATCAAATTCA
AAGCCTCAAAAAATTATTAAAAAATCGAGCGCTAGAAAATAAACACTATG
ATGCTTCGAGTATATTGGTTACCTCCTCTCGGCATCGAGACGCTCTTCAG
AAAACACAAGAGCATATTCAATCAGCTTTAATAGGGTTAGCTCGCGGAAT
GACAGGAGATTTCCTTTCTATAGATCTGCGCGCTGCTTTGAATGAGTTGG
GAACCGTAACTGGTGAGATCACCAACGAACATGTTCTAGATTCTATCTTT
TCGCGGTTTTGTATCGGAAAGTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig106002:3..476+

>mRNA_M-pyrifera_M_contig106002.1284.1 ID=mRNA_M-pyrifera_M_contig106002.1284.1|Name=mRNA_M-pyrifera_M_contig106002.1284.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=948bp|location=Sequence derived from alignment at M-pyrifera_M_contig106002:3..476+ (Macrocystis pyrifera P11B4 male)
CAAGCCGACCTTGTTATTTACTTAAAGGATTTATCAAGTCCATTTAGTGA
TGAAGAACGAAAAGAAATTGCCGAATTTCAGCAACGAGCTGGTGAAACCC
CATTTATTTTGATTGGAACCAAAGCAGATATCGAAGCCGATAAAGAATGG
CGGACAGAGTTTGATGTAAAAATATCTGCATTGAAAGGGGATCAAATTCA
AAGCCTCAAAAAATTATTAAAAAATCGAGCGCTAGAAAATAAACACTATG
ATGCTTCGAGTATATTGGTTACCTCCTCTCGGCATCGAGACGCTCTTCAG
AAAACACAAGAGCATATTCAATCAGCTTTAATAGGGTTAGCTCGCGGAAT
GACAGGAGATTTCCTTTCTATAGATCTGCGCGCTGCTTTGAATGAGTTGG
GAACCGTAACTGGTGAGATCACCAACGAACATGTTCTAGATTCTATCTTT
TCGCGGTTTTGTATCGGAAAGTGACAAGCCGACCTTGTTATTTACTTAAA
GGATTTATCAAGTCCATTTAGTGATGAAGAACGAAAAGAAATTGCCGAAT
TTCAGCAACGAGCTGGTGAAACCCCATTTATTTTGATTGGAACCAAAGCA
GATATCGAAGCCGATAAAGAATGGCGGACAGAGTTTGATGTAAAAATATC
TGCATTGAAAGGGGATCAAATTCAAAGCCTCAAAAAATTATTAAAAAATC
GAGCGCTAGAAAATAAACACTATGATGCTTCGAGTATATTGGTTACCTCC
TCTCGGCATCGAGACGCTCTTCAGAAAACACAAGAGCATATTCAATCAGC
TTTAATAGGGTTAGCTCGCGGAATGACAGGAGATTTCCTTTCTATAGATC
TGCGCGCTGCTTTGAATGAGTTGGGAACCGTAACTGGTGAGATCACCAAC
GAACATGTTCTAGATTCTATCTTTTCGCGGTTTTGTATCGGAAAGTGA
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