mRNA_M-pyrifera_M_contig105925.1270.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig105925.1270.1
Unique NamemRNA_M-pyrifera_M_contig105925.1270.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig105925.1270.1 vs. uniprot
Match: A0A6N7FRV5_9ACTN (Flagellar hook-associated protein 2 n=1 Tax=Acidimicrobiia bacterium TaxID=2080302 RepID=A0A6N7FRV5_9ACTN)

HSP 1 Score: 129 bits (324), Expect = 8.360e-32
Identity = 80/208 (38.46%), Postives = 122/208 (58.65%), Query Frame = 1
Query:    1 GEDAEIRIEGTSPFTITSSDDVFEDLIPGVDNTLTGVPDGEVTVDVTRDSAALAGRVKALVDAVNSVLEGLSAASTYDADENQASLLTGNSTVRRARDELTAALINPVSGAALGSVGLTGVTISREGTFEFDQTVFQEAYKKDPAAVQRVFAVPFGSSEESAMSRMLEEVDDATAFGTGYLRSAEDSEKAQVEDLAESITKWDQRLEI 624
            G DAEI + G   +T TS  + F+DL+P VD T+       +TV VT D+ A+A R +ALV+AVN  L  +   + YDA   ++S LTG+ +V+R   ELT  L+ PV G  L    L G+T+ R+GT  FD+  F+EA   +P AVQ +F    G+ + + ++R+ E    A   GTGYL +A++S++ +V+     I   ++RLE+
Sbjct:  207 GNDAEIEMAG-GLYTATSPTNTFDDLLPNVDVTVKAESTEPITVSVTTDTDAIANRAQALVNAVNGTLSSVKTLTAYDATAKRSSALTGDHSVQRLSQELTRGLVGPVDGTDLIPATL-GITVDRDGTIAFDRVKFEEALADNPEAVQGLFVQAEGAPD-NMITRLAEVAKGAVETGTGYLLTAQESQRDRVKSFTTQIEDIERRLEL 411          
BLAST of mRNA_M-pyrifera_M_contig105925.1270.1 vs. uniprot
Match: A0A5Q2RKP9_9ACTN (Flagellar hook-associated protein 2 n=1 Tax=Actinomarinicola tropica TaxID=2789776 RepID=A0A5Q2RKP9_9ACTN)

HSP 1 Score: 116 bits (291), Expect = 8.240e-27
Identity = 67/148 (45.27%), Postives = 94/148 (63.51%), Query Frame = 1
Query:   43 TITSSDDVFEDLIPGVDNTLTGVPDGEVTVDVTRDSAALAGRVKALVDAVNSVLEGLSAASTYDADENQASLLTGNSTVRRARDELTAALINPVSGAALGSVGLTGVTISREGTFEFDQTVFQEAYKKDPAAVQRVFAVPFGSSEESA 486
            T+T   +  +DL+ GV  TL     G VTV+V RD+  +  +VKALV+AVN  L  +   + YDA+ NQ S LTG++TVR+    LT AL +PV+ ++LGSVGL GV ++R+G   FD+  F+ AY  DPAAV+R+F    G +  SA
Sbjct:  211 TVTRPTNTIDDLVGGVTLTLKAETTGPVTVNVARDADGITKKVKALVEAVNGALAEMKIRTAYDAETNQRSSLTGDATVRQLTGLLTGALADPVAASSLGSVGLAGVELTRDGKVTFDEAEFKAAYAADPAAVERLFVA--GGTSTSA 356          
BLAST of mRNA_M-pyrifera_M_contig105925.1270.1 vs. uniprot
Match: UPI001D1213B3 (flagellar filament capping protein FliD n=1 Tax=Aquihabitans sp. Kera 3 TaxID=2884263 RepID=UPI001D1213B3)

HSP 1 Score: 105 bits (262), Expect = 9.700e-23
Identity = 61/152 (40.13%), Postives = 89/152 (58.55%), Query Frame = 1
Query:    1 GEDAEIRI-EGTSPFTITSSDDVFEDLIPGVDNTLTGVPDGEVTVDVTRDSAALAGRVKALVDAVNSVLEGLSAASTYDADENQASLLTGNSTVRRARDELTAALINPVSGAALGSVGLTGVTISREGTFEFDQTVFQEAYKKDPAAVQRVF 453
            G+DA++ +  G   + +TS  + F D++ GV  T   V    VTVDV  D   +A +V++ VD++N+VL  +   + YDA    A+ L G+ TVRR   EL  A+ + V  ++LGS GL G+ I+R GTF FD   F  AY+ DP AVQ +F
Sbjct:  197 GQDAKLVVGSGPGAYDVTSPSNTFSDVLSGVSITAVAVSASPVTVDVAEDVDGMAKQVQSFVDSLNAVLGEIKTRTAYDAATKTAASLNGDPTVRRVAQELIRAVTDQVGASSLGSAGLAGLQITRNGTFTFDSAKFGAAYRDDPDAVQALF 348          
BLAST of mRNA_M-pyrifera_M_contig105925.1270.1 vs. uniprot
Match: UPI000F078DF9 (flagellar filament capping protein FliD n=1 Tax=unclassified Nocardioides TaxID=2615069 RepID=UPI000F078DF9)

HSP 1 Score: 102 bits (254), Expect = 6.010e-22
Identity = 72/196 (36.73%), Postives = 104/196 (53.06%), Query Frame = 1
Query:   46 ITSSDDVFEDLIPGVDNTLTGVPDGEVTVDVT--RDSAALAGRVKALVDAVNSVLEGLSAASTYDADENQASLLTGNSTVRRARDELTAALINPVSGAALGSVGLTGVTISREGTFEFDQTVFQEAYKKDPAAVQRVFAVPFGSSEESAMSRMLEEVDDATAFGTGYLRSAEDSEKAQVEDLAESITKWDQRLEIR 627
            +TS+ + FEDL+PG+D T++    G   V VT  RD  AL+  VKALVDA N++L G+ + + YD+       L G+STVR  R++L  + I P  G +L  VG   + + R G   FD   F  AY  DPA V    A  F ++     +R+ +   D +   TG L +A     A ++ L +SI  W  RLE+R
Sbjct:  215 VTSATNTFEDLVPGLDVTVSAAAVGTTPVSVTVARDPKALSDSVKALVDAANAILTGIGSQTAYDSKTGSGGQLLGDSTVRALRNDLLES-IYPADGTSLAGVG---IQVDRYGKLVFDADAFASAYAADPAGV----AAKFDTAGNGFAARVEKVGKDYSDPYTGTLTAAITGRTAGIDRLEDSIEAWSVRLELR 402          
BLAST of mRNA_M-pyrifera_M_contig105925.1270.1 vs. uniprot
Match: A0A2E8YXC5_9ACTN (Flagellar hook-associated protein 2 n=1 Tax=Nocardioides sp. TaxID=35761 RepID=A0A2E8YXC5_9ACTN)

HSP 1 Score: 102 bits (254), Expect = 6.190e-22
Identity = 74/214 (34.58%), Postives = 117/214 (54.67%), Query Frame = 1
Query:    1 GEDAEIRIEGTSPFTITSSDDVFEDLIPGVDNTLTG--VPDGEVTVDVTRDSAALAGRVKALVDAVNSVLEGLSAASTYDADENQASLLTGNSTVRRARDELTAALINPVSGAALGSVGLTGVTISREGTFEFDQTVFQEAYKKDPAAVQRVFAVPFGSSEESAMSRMLEEVDDATAFGTGY---LRSAEDSEKAQVEDLAESITKWDQRLEIR 627
            G DA I +   +   +TS+   F+D++PGV  +L G       VTV VTRD+A ++G+VKALVDAVN+ L  +   +   AD + +S LTG+ TVR+ R +L    I    G +L + G   +  +R+G  EFD+T F+ AY  DP     +F    GS   + ++ +      ATA    Y   L +A    ++ ++ + +S+ +WD+RLE+R
Sbjct:  207 GSDASIDLG--AGLVVTSASGTFDDVVPGVSLSLAGSATVGTVVTVGVTRDTAGMSGKVKALVDAVNAALAEIDVKTAVSADGSTSSPLTGDGTVRQLRSQLLDT-IRTTGGTSLAAYG---IQTTRDGRLEFDETTFKAAYAADPTGTADMFTTA-GSGFAAKVASV------ATAASDPYDGSLSTAITGRQSGIDRMEDSVDEWDRRLELR 407          
BLAST of mRNA_M-pyrifera_M_contig105925.1270.1 vs. uniprot
Match: A0A7Z8JZY9_9CELL (Flagellar hook-associated protein 2 n=6 Tax=Micrococcales TaxID=85006 RepID=A0A7Z8JZY9_9CELL)

HSP 1 Score: 98.6 bits (244), Expect = 1.590e-20
Identity = 73/201 (36.32%), Postives = 109/201 (54.23%), Query Frame = 1
Query:   46 ITSSDDVFEDLIPGVDNTLTGVPDGEVTVDVTRDSAALAGRVKALVDAVNSVLEGLSAASTYDADENQ-------ASLLTGNSTVRRARDELTAALINPVSGAALGSVGLTGVTISREGTFEFDQTVFQEAYKKDPAAVQRVFAVPFGSSEESAMSRMLEEVDDATAFGTGYLRSAEDSEKAQVEDLAESITKWDQRLEIR 627
            +TS+ +VFE+L+ GV+ T+T      VTVDVTRD + L+   +ALV+ +N+VL  +++ +T     N        A L +GN+TVR  +  L AA  N  S  +L  +G   +++ ++GTF FD   FQ A  KDP AV +      GS ++        EV   +A GT  L+    S  ++V+DL + I  WD RLE R
Sbjct:  225 VTSAKNVFENLVTGVNVTVTAKTTDPVTVDVTRDDSKLSALAQALVNNLNAVLGDITSYTTSKTGTNDEGTAIVTAGLFSGNATVRGLQQSLLAAGSNSASNTSLAGIG---ISLGKDGTFTFDAAAFQTALTKDPDAVGKAVQGVAGSLQKVG------EVASDSAKGTLTLQVQ--SYNSEVKDLTDRIADWDIRLEAR 414          
BLAST of mRNA_M-pyrifera_M_contig105925.1270.1 vs. uniprot
Match: A0A1G8BUT5_9MICO (Flagellar hook-associated protein 2 n=1 Tax=Microbacterium sp. 77mftsu3.1 TaxID=1761802 RepID=A0A1G8BUT5_9MICO)

HSP 1 Score: 93.6 bits (231), Expect = 9.840e-19
Identity = 67/216 (31.02%), Postives = 111/216 (51.39%), Query Frame = 1
Query:    1 GEDAEIRI-EGT-SPFTITSSDDVFEDLIPGVDNTLTGVPDGEVTVDVTRDSAALAGRVKALVDAVNSVLEGLSAAS-----TYDADENQASLLTGNSTVRRARDELTAALINPVSGAALGSVGLTGVTISREGTFEFDQTVFQEAYKKDPAAVQRVFAVPFGSSEESAMSRMLEEVDDATAFGTGYLRSAEDSEKAQVEDLAESITKWDQRLEIR 627
            G DA++R+  GT +   +TS  + F DL+PGVD T++ VPD  V + V  D+AA     +  V  + +++ GL+  S     T   +  +  + TG+ST+R+ R  L  A+  PV G +  ++G   ++  + G  +FD+  F  A +KDPAAV +VF        +   SR+    DD +    G + S    ++ +V  + + +  WD RLE R
Sbjct:  212 GRDAQVRLWAGTDAEQVVTSKTNTFTDLLPGVDITISKVPDAAVDITVATDTAARTTAAETYVKQIQALMTGLANGSKATTATAPGETTRLGVFTGDSTIRQLRTSLANAVQYPVDGVSPSTIG---ISFDKFGALQFDKEKFTAAIEKDPAAVDKVF--------KGISSRVQGVADDYSDKYDGLITSKITGQETEVRAMKKQVESWDVRLEQR 416          
BLAST of mRNA_M-pyrifera_M_contig105925.1270.1 vs. uniprot
Match: UPI00177FFD63 (flagellar filament capping protein FliD n=1 Tax=Microbacterium sp. Sa4CUA7 TaxID=2762236 RepID=UPI00177FFD63)

HSP 1 Score: 91.7 bits (226), Expect = 4.840e-18
Identity = 66/216 (30.56%), Postives = 109/216 (50.46%), Query Frame = 1
Query:    1 GEDAEIRIEGTSP--FTITSSDDVFEDLIPGVDNTLTGVPDGEVTVDVTRDSAALAGRVKALVDAVNSVLEGLS-----AASTYDADENQASLLTGNSTVRRARDELTAALINPVSGAALGSVGLTGVTISREGTFEFDQTVFQEAYKKDPAAVQRVFAVPFGSSEESAMSRMLEEVDDATAFGTGYLRSAEDSEKAQVEDLAESITKWDQRLEIR 627
            G+DA++R+   +P   T+TS D+ F DL PGVD T++      +TV V+ D AA A      V  + +VL G++      A T         + TG+STVR  +  LT A+ +PV   +  ++G   ++I R G   FD+  F  A + D A+V+ VFA           +R+ +  D  +    G + +    ++ +V  +A+ + +WD RLE+R
Sbjct:  217 GQDAQVRLWAGTPAEMTVTSDDNTFTDLFPGVDITVSKASTAPITVSVSADPAARAKAAGEFVSQIKAVLTGIANGSKATAPTTAGGSTTLGVFTGDSTVRALQLALTNAVQHPVDAVSPSTIG---ISIDRYGVLSFDEKKFTAALEADEASVEAVFA--------GVAARVQQTTDQYSDKYDGLVTARITGQEGEVNRIADQLERWDVRLEMR 421          
BLAST of mRNA_M-pyrifera_M_contig105925.1270.1 vs. uniprot
Match: UPI001B3132F3 (flagellar filament capping protein FliD n=1 Tax=Naasia sp. SYSU D00057 TaxID=2817380 RepID=UPI001B3132F3)

HSP 1 Score: 91.7 bits (226), Expect = 4.880e-18
Identity = 65/202 (32.18%), Postives = 104/202 (51.49%), Query Frame = 1
Query:   43 TITSSDDVFEDLIPGVDNTLTGVPDGEVTVDVTRDSAALAGRVKALVDAVNSVLEGLSAASTYDADENQASL-------LTGNSTVRRARDELTAALINPVSGAALGSVGLTGVTISREGTFEFDQTVFQEAYKKDPAAVQRVFAVPFGSSEESAMSRMLEEVDDATAFGTGYLRSAEDSEKAQVEDLAESITKWDQRLEIR 627
            T+TSS + F +++ G D T+       VTV V +D  A +      V  ++++   +S  S      N A          TG+STVR+ +D L  A+I+PV G +L ++G   ++++R+G+  FD   F EA K DPA  QR+F+        +A +R+ E  +DA+    G+L S    ++  V+ L E+I  WD RL  R
Sbjct:  234 TLTSSTNTFAEVLGGTDITVAKAATEPVTVTVAQDGNAASAVASGFVAKLSALFGTISVKSKVTPSTNAAGTPIVSAGSFTGDSTVRQLKDALLTAVIDPVEGKSLSTIG---ISVTRDGSVSFDAAKFGEALKSDPAGTQRMFS--------AAAARVQEVAEDASDSLDGFLTSKVTGQENAVKSLNENIAAWDLRLAKR 424          
BLAST of mRNA_M-pyrifera_M_contig105925.1270.1 vs. uniprot
Match: UPI0003709E87 (flagellar filament capping protein FliD n=1 Tax=Actinokineospora enzanensis TaxID=155975 RepID=UPI0003709E87)

HSP 1 Score: 91.3 bits (225), Expect = 5.770e-18
Identity = 67/193 (34.72%), Postives = 92/193 (47.67%), Query Frame = 1
Query:   40 FTITSSDDVFEDLIPGVDNTLTGVPDGEVTVDVTRDSAALAGRVKALVDAVNSVLEGLSAASTYDADENQASLLTGNSTVRRARDELTAALINPVSGAALGSVGLTGVTISREGTFEFDQTVFQEAYKKDPAAVQRVFAVPFGSSEESAMSRMLEEVDDATAFGTGYLRSAEDSEKAQVEDLAESITKWDQRL 618
            F ITS+D+ +  LIPGV  T   V  G +TV  + DS+     ++ALV AVNS L  + AAS Y+     ++ L GN+ VR  RD+L  A+     G    S    GV++ R G  +FD T FQ +   DP   +   +   G    S +S      DD T   TG L     +    V  L + I+ WD RL
Sbjct:  211 FKITSADNTYTGLIPGVTVTANKVDTG-ITVSTSPDSSKATSAMQALVSAVNSALSTIDAASAYNPTTKTSAPLAGNTLVRSLRDQLLGAVSKGTDGGT--SFAANGVSLDRNGQVKFDATAFQNSLAADPTGTKAALSTTLGG-RFSTIS------DDTTNSTTGKLTLVIQNGNTYVRRLTDQISDWDDRL 393          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig105925.1270.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6N7FRV5_9ACTN8.360e-3238.46Flagellar hook-associated protein 2 n=1 Tax=Acidim... [more]
A0A5Q2RKP9_9ACTN8.240e-2745.27Flagellar hook-associated protein 2 n=1 Tax=Actino... [more]
UPI001D1213B39.700e-2340.13flagellar filament capping protein FliD n=1 Tax=Aq... [more]
UPI000F078DF96.010e-2236.73flagellar filament capping protein FliD n=1 Tax=un... [more]
A0A2E8YXC5_9ACTN6.190e-2234.58Flagellar hook-associated protein 2 n=1 Tax=Nocard... [more]
A0A7Z8JZY9_9CELL1.590e-2036.32Flagellar hook-associated protein 2 n=6 Tax=Microc... [more]
A0A1G8BUT5_9MICO9.840e-1931.02Flagellar hook-associated protein 2 n=1 Tax=Microb... [more]
UPI00177FFD634.840e-1830.56flagellar filament capping protein FliD n=1 Tax=Mi... [more]
UPI001B3132F34.880e-1832.18flagellar filament capping protein FliD n=1 Tax=Na... [more]
UPI0003709E875.770e-1834.72flagellar filament capping protein FliD n=1 Tax=Ac... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig105925contigM-pyrifera_M_contig105925:5..631 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score99.4
Seed ortholog evalue2.2e-18
Seed eggNOG ortholog1349820.M707_17260
Preferred namefliD
KEGG koko:K02407
KEGG Pathwayko02040,map02040
Hectar predicted targeting categoryother localisation
GOsGO:0001539,GO:0003674,GO:0005198,GO:0005575,GO:0005576,GO:0005623,GO:0006928,GO:0008150,GO:0009288,GO:0009420,GO:0009421,GO:0009424,GO:0009987,GO:0040011,GO:0042995,GO:0043226,GO:0043228,GO:0044422,GO:0044461,GO:0044463,GO:0044464,GO:0048870,GO:0051179,GO:0051674,GO:0071973,GO:0071978,GO:0097588
EggNOG free text desc.Required for morphogenesis and for the elongation of the flagellar filament by facilitating polymerization of the flagellin monomers at the tip of growing filament. Forms a capping structure, which prevents flagellin subunits (transported through the central channel of the flagellum) from leaking out without polymerization at the distal end
EggNOG OGs2IBVB@201174,COG1345@1,COG1345@2
COG Functional cat.N
Best tax levelActinobacteria
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko02035
Exons1
Model size627
Cds size627
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815568.3812191-CDS-M-pyrifera_M_contig105925:4..6311622815568.3812191-CDS-M-pyrifera_M_contig105925:4..631Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig105925 5..631 -
1692277467.6642034-CDS-M-pyrifera_M_contig105925:4..6311692277467.6642034-CDS-M-pyrifera_M_contig105925:4..631Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig105925 5..631 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig105925.1270.1prot_M-pyrifera_M_contig105925.1270.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig105925 5..631 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig105925.1270.1

>prot_M-pyrifera_M_contig105925.1270.1 ID=prot_M-pyrifera_M_contig105925.1270.1|Name=mRNA_M-pyrifera_M_contig105925.1270.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=209bp
GEDAEIRIEGTSPFTITSSDDVFEDLIPGVDNTLTGVPDGEVTVDVTRDS
AALAGRVKALVDAVNSVLEGLSAASTYDADENQASLLTGNSTVRRARDEL
TAALINPVSGAALGSVGLTGVTISREGTFEFDQTVFQEAYKKDPAAVQRV
FAVPFGSSEESAMSRMLEEVDDATAFGTGYLRSAEDSEKAQVEDLAESIT
KWDQRLEIR
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mRNA from alignment at M-pyrifera_M_contig105925:5..631-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig105925.1270.1 ID=mRNA_M-pyrifera_M_contig105925.1270.1|Name=mRNA_M-pyrifera_M_contig105925.1270.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=627bp|location=Sequence derived from alignment at M-pyrifera_M_contig105925:5..631- (Macrocystis pyrifera P11B4 male)
GGCGAGGACGCCGAGATTCGGATCGAGGGCACGAGCCCGTTCACCATCAC CTCGTCCGACGATGTCTTCGAAGACCTCATCCCCGGCGTCGACAACACGC TCACCGGCGTCCCCGACGGCGAGGTCACCGTCGATGTCACCCGCGACTCG GCAGCTCTCGCCGGCCGGGTGAAGGCGCTCGTCGATGCCGTCAACTCGGT GCTCGAGGGCCTGTCCGCCGCCTCCACCTACGACGCCGACGAGAACCAGG CGAGTCTGCTCACCGGCAACAGCACGGTGCGCCGGGCCCGCGACGAGCTC ACCGCCGCCCTGATCAACCCCGTGAGCGGCGCGGCGCTCGGCTCGGTCGG CCTCACCGGCGTGACGATCAGCCGCGAGGGCACGTTCGAGTTCGACCAGA CCGTCTTCCAGGAGGCCTACAAGAAGGACCCGGCCGCCGTGCAGCGGGTC TTCGCGGTGCCCTTCGGCAGCAGCGAAGAGTCGGCGATGTCGCGGATGTT GGAGGAGGTCGACGACGCCACCGCGTTCGGGACCGGCTATCTGCGCAGCG CAGAGGACTCCGAAAAGGCCCAGGTCGAGGATCTCGCCGAGTCCATCACG AAGTGGGACCAGAGACTCGAGATTCGC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig105925:5..631-

>mRNA_M-pyrifera_M_contig105925.1270.1 ID=mRNA_M-pyrifera_M_contig105925.1270.1|Name=mRNA_M-pyrifera_M_contig105925.1270.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1254bp|location=Sequence derived from alignment at M-pyrifera_M_contig105925:5..631- (Macrocystis pyrifera P11B4 male)
GGCGAGGACGCCGAGATTCGGATCGAGGGCACGAGCCCGTTCACCATCAC
CTCGTCCGACGATGTCTTCGAAGACCTCATCCCCGGCGTCGACAACACGC
TCACCGGCGTCCCCGACGGCGAGGTCACCGTCGATGTCACCCGCGACTCG
GCAGCTCTCGCCGGCCGGGTGAAGGCGCTCGTCGATGCCGTCAACTCGGT
GCTCGAGGGCCTGTCCGCCGCCTCCACCTACGACGCCGACGAGAACCAGG
CGAGTCTGCTCACCGGCAACAGCACGGTGCGCCGGGCCCGCGACGAGCTC
ACCGCCGCCCTGATCAACCCCGTGAGCGGCGCGGCGCTCGGCTCGGTCGG
CCTCACCGGCGTGACGATCAGCCGCGAGGGCACGTTCGAGTTCGACCAGA
CCGTCTTCCAGGAGGCCTACAAGAAGGACCCGGCCGCCGTGCAGCGGGTC
TTCGCGGTGCCCTTCGGCAGCAGCGAAGAGTCGGCGATGTCGCGGATGTT
GGAGGAGGTCGACGACGCCACCGCGTTCGGGACCGGCTATCTGCGCAGCG
CAGAGGACTCCGAAAAGGCCCAGGTCGAGGATCTCGCCGAGTCCATCACG
AAGTGGGACCAGAGACTCGAGATTCGCGGCGAGGACGCCGAGATTCGGAT
CGAGGGCACGAGCCCGTTCACCATCACCTCGTCCGACGATGTCTTCGAAG
ACCTCATCCCCGGCGTCGACAACACGCTCACCGGCGTCCCCGACGGCGAG
GTCACCGTCGATGTCACCCGCGACTCGGCAGCTCTCGCCGGCCGGGTGAA
GGCGCTCGTCGATGCCGTCAACTCGGTGCTCGAGGGCCTGTCCGCCGCCT
CCACCTACGACGCCGACGAGAACCAGGCGAGTCTGCTCACCGGCAACAGC
ACGGTGCGCCGGGCCCGCGACGAGCTCACCGCCGCCCTGATCAACCCCGT
GAGCGGCGCGGCGCTCGGCTCGGTCGGCCTCACCGGCGTGACGATCAGCC
GCGAGGGCACGTTCGAGTTCGACCAGACCGTCTTCCAGGAGGCCTACAAG
AAGGACCCGGCCGCCGTGCAGCGGGTCTTCGCGGTGCCCTTCGGCAGCAG
CGAAGAGTCGGCGATGTCGCGGATGTTGGAGGAGGTCGACGACGCCACCG
CGTTCGGGACCGGCTATCTGCGCAGCGCAGAGGACTCCGAAAAGGCCCAG
GTCGAGGATCTCGCCGAGTCCATCACGAAGTGGGACCAGAGACTCGAGAT
TCGC
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