mRNA_M-pyrifera_M_contig104979.1070.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig104979.1070.1
Unique NamemRNA_M-pyrifera_M_contig104979.1070.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig104979.1070.1 vs. uniprot
Match: A0A5D6XXW0_9STRA (Uncharacterized protein n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6XXW0_9STRA)

HSP 1 Score: 109 bits (272), Expect = 5.370e-26
Identity = 67/173 (38.73%), Postives = 86/173 (49.71%), Query Frame = 1
Query:   16 FMRSVYLCNKLVVAAVNGHAVGAGVTVLPHCDLVVVVEGGKLWTPFVALGIVPELSSSTTFPSILGSARGKAALLAGRKLSSEELYHCGFASHILPPTTSFLSNVLH----LAASTAYATRDAPAAIKDAKVL------IRSPHTLETAYKEYKILAKRVNEGMAMAAAMETI 504
            FM ++  C KL+VAAVNG A+G GVT+L HCD+V   +  K WTPF+ +GIVPE +SS TFP+ILG       LL  +++  E    CG  S + P    FL  V      L A         P A K   +       +R P   E    EY  L +R   G   AAA +TI
Sbjct:   99 FMHTMLRCRKLIVAAVNGRAIGIGVTLLMHCDIVFAADSAKFWTPFMRIGIVPEFASSYTFPTILGPMVANDLLLRSKEIGIERAVACGLVSEVFPAGPGFLQRVFDEIEPLVAE--------PVAAKTLSLFKSVIRRVREPLVREALVAEYVELDRRFLSGETYAAASKTI 263          
BLAST of mRNA_M-pyrifera_M_contig104979.1070.1 vs. uniprot
Match: UPI0019A0DAFB (Enoyl-CoA hydratase n=1 Tax=Chitinophagaceae bacterium TaxID=1869212 RepID=UPI0019A0DAFB)

HSP 1 Score: 105 bits (261), Expect = 1.590e-24
Identity = 69/176 (39.20%), Postives = 95/176 (53.98%), Query Frame = 1
Query:    1 APPNVFMRSVYLCNKLVVAAVNGHAVGAGVTVLPHCDLVVVVEGGKLWTPFVALGIVPELSSSTTFPSILGSARGKAALLAGRKLSSEELYHCGFASHILPPTTSFLSNVLHLAASTAYATRDAP-AAIKDAKVLIRSPH---TLETAYKEYKILAKRVNEGMAMAAAMETIQRRK 516
            +P   FMR++  C+K VVAAV G A+G G T+L HCD V V +  +L  PFVALG+VPE +SS   P ++G  R    LL G   + E+   CG AS +LP      S V++ A   A      P  A+++AK L+R+P    TL+T   E  +   R+    AM A     Q+RK
Sbjct:   84 SPVFQFMRALIDCDKPVVAAVTGAAIGIGTTMLLHCDFVYVSDEARLAMPFVALGLVPEFASSLLLPQLMGQRRAAEKLLLGDPFTPEQAVDCGIASAVLPA-----SEVVNHARRIAERFNSLPPGAVREAKQLLRAPQREATLQTIRSEGALFTSRLRSPEAMEAFQAFFQKRK 254          
BLAST of mRNA_M-pyrifera_M_contig104979.1070.1 vs. uniprot
Match: UPI001F538583 (enoyl-CoA hydratase-related protein n=2 Tax=Calidifontimicrobium TaxID=2828371 RepID=UPI001F538583)

HSP 1 Score: 103 bits (257), Expect = 6.060e-24
Identity = 67/170 (39.41%), Postives = 91/170 (53.53%), Query Frame = 1
Query:   16 FMRSVYLCNKLVVAAVNGHAVGAGVTVLPHCDLVVVVEGGKLWTPFVALGIVPELSSSTTFPSILGSARGKAALLAGRKLSSEELYHCGFASHILPPTTSFLSNVLHLAASTAYATRDAP-AAIKDAKVLIRSPH---TLETAYKEYKILAKRVNEGMAMAAAMETIQRR 513
            FMR++  C+K VVAAV G AVG G T+L HCD V V +  +L  PFV LG+VPE +SS   P ++G  R    LL G    +EE   CG A+ +LPP     + VL  A   A      P  A++DAK L+R+P     ++    E +I  +R+    AM A     Q+R
Sbjct:   88 FMRALLECDKPVVAAVTGAAVGIGTTMLLHCDFVYVADDARLAMPFVGLGLVPEYASSLLVPQLMGPRRAAEKLLLGDPFGAEEAVACGIANAVLPP-----AEVLPHARRVAERFNALPPGAVRDAKALMRAPQREQVMQAIAAEAEIFTRRLRSPEAMEAFQAFFQKR 252          
BLAST of mRNA_M-pyrifera_M_contig104979.1070.1 vs. uniprot
Match: A0A7C9PJN2_9BURK (Enoyl-CoA hydratase n=1 Tax=Ideonella livida TaxID=2707176 RepID=A0A7C9PJN2_9BURK)

HSP 1 Score: 101 bits (252), Expect = 2.990e-23
Identity = 65/175 (37.14%), Postives = 93/175 (53.14%), Query Frame = 1
Query:    1 APPNVFMRSVYLCNKLVVAAVNGHAVGAGVTVLPHCDLVVVVEGGKLWTPFVALGIVPELSSSTTFPSILGSARGKAALLAGRKLSSEELYHCGFASHILPPTTSFLSNVLHLAASTAYATRDAPAAIKDAKVLIRSPHT---LETAYKEYKILAKRVNEGMAMAAAMETIQRRK 516
            +P   FM+++  C+K VVAAV G AVG G T+L HCD V V +  +L  PFV LG+VPE  SS   P ++G  +G   LL G   + +E   CG A+ +LP        V H         +  PAA+++AK L+R+PH    L T   E ++ ++R+    A  A     Q+RK
Sbjct:   77 SPVLQFMKALMDCDKPVVAAVTGAAVGIGTTLLLHCDFVYVSDEARLAMPFVGLGLVPEFGSSLLVPQLMGLRKGAEKLLLGDPFTPDEAVECGIANAVLPAGEV----VAHARRVAERFNQLPPAAVREAKRLMRAPHKEQLLHTILTESQLFSERLRSPEATEAFQAFFQKRK 247          
BLAST of mRNA_M-pyrifera_M_contig104979.1070.1 vs. uniprot
Match: A0A0N1L0Y5_9PROT (Enoyl-CoA hydratase n=3 Tax=Betaproteobacteria TaxID=28216 RepID=A0A0N1L0Y5_9PROT)

HSP 1 Score: 101 bits (252), Expect = 3.370e-23
Identity = 65/174 (37.36%), Postives = 92/174 (52.87%), Query Frame = 1
Query:    1 APPNVFMRSVYLCNKLVVAAVNGHAVGAGVTVLPHCDLVVVVEGGKLWTPFVALGIVPELSSSTTFPSILGSARGKAALLAGRKLSSEELYHCGFASHILPPTTSFLSNVLHLAASTAYATRDAPAAIKDAKVLIRSPH---TLETAYKEYKILAKRVNEGMAMAAAMETIQRR 513
            +P   FMR++  C+K VVAAV G A+G G T+L HCD V V +  +L  PFV LG+VPE +SS   P ++G+ R    LL G   + E+   CG A+ +LP        V H            P A+++AK L+R+P     L+T   E ++ AKR+    AM A     Q+R
Sbjct:   84 SPVFKFMRALLDCDKPVVAAVTGAAIGIGTTLLLHCDFVYVSDEARLAMPFVGLGLVPEFASSLVVPQLMGNRRAAEKLLLGDPFTPEQAVECGIANAVLPAQEV----VNHARRVAERFNALPPGAVREAKQLMRAPQRELVLQTIRTEGELFAKRLRSPEAMEAFQAFFQKR 253          
BLAST of mRNA_M-pyrifera_M_contig104979.1070.1 vs. uniprot
Match: A0A835ZBJ2_9STRA (ClpP/crotonase-like domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZBJ2_9STRA)

HSP 1 Score: 98.6 bits (244), Expect = 7.210e-23
Identity = 52/104 (50.00%), Postives = 66/104 (63.46%), Query Frame = 1
Query:   19 MRSVYLCNKLVVAAVNGHAVGAGVTVLPHCDLVVVVEGGKLWTPFVALGIVPELSSSTTFPSILGSARGKAALLAGRKLSSEELYHCGFASHILPPTTSFLSNV 330
            M++V    KL+VAAVNG AVG GVT+LPHCDLVV  +    WTPF  L IVPEL SS TFP ++G ++    L+ G+KLS++E       S I P +  FL  V
Sbjct:    1 MKAVVHFRKLLVAAVNGPAVGIGVTMLPHCDLVVCAQKATFWTPFGRLAIVPELCSSVTFPELMGYSKATEMLVLGQKLSAKEALQAKLVSRIYP-SEGFLEQV 103          
BLAST of mRNA_M-pyrifera_M_contig104979.1070.1 vs. uniprot
Match: D0N4V4_PHYIT (Enoyl-CoA hydratase/isomerase family, putative n=12 Tax=Phytophthora TaxID=4783 RepID=D0N4V4_PHYIT)

HSP 1 Score: 100 bits (248), Expect = 1.550e-22
Identity = 59/169 (34.91%), Postives = 89/169 (52.66%), Query Frame = 1
Query:    1 APPNVFMRSVYLCNKLVVAAVNGHAVGAGVTVLPHCDLVVVVEGGKLWTPFVALGIVPELSSSTTFPSILGSARGKAALLAGRKLSSEELYHCGFASHILPPTTSFLSNVLHLAASTAYATRDAPAAIKDAKVLIR---SPHTLETAYKEYKILAKRVNEGMAMAAAME 498
            +P + FM  +  CNKL++AAVNG A+G GVT+L HCDLV   +    WTPF+ +G+VPE +SS TFP +LG       ++  +  +++E         + P TT FL  VL    +     R    ++   K L+R   +P   E  + E++ L +R   G  +A  ME
Sbjct:   91 SPSHTFMHKMLDCNKLLIAAVNGPAIGIGVTLLMHCDLVYAADSATFWTPFLRIGVVPEFASSYTFPHLLGPIVANNLVIRSKVYTAQEALSTNIVGEVFP-TTGFLEKVL-AELTPLVNNRFNKTSLPVYKTLLRRERAPKIREALFYEFEQLDRRAASGEFLATVME 257          
BLAST of mRNA_M-pyrifera_M_contig104979.1070.1 vs. uniprot
Match: UPI0013D6EB51 (enoyl-CoA hydratase-related protein n=1 Tax=Pseudooceanicola aestuarii TaxID=2697319 RepID=UPI0013D6EB51)

HSP 1 Score: 99.8 bits (247), Expect = 1.560e-22
Identity = 57/131 (43.51%), Postives = 77/131 (58.78%), Query Frame = 1
Query:   16 FMRSVYLCNKLVVAAVNGHAVGAGVTVLPHCDLVVVVEGGKLWTPFVALGIVPELSSSTTFPSILGSARGKAALLAGRKLSSEELYHCGFASHILPPTTSFLSNVLHLAASTAYATRDAPAAIKDAKVLIR 408
            F+ ++  C K V+AAVNG A+G GVT+L HCDLVV+ E     TPFV LG+VPE  SS   P+++G A     LLA R L++ E +  G AS ++P      +    LAA  A     AP A+++ K LIR
Sbjct:   84 FLTALSTCPKPVIAAVNGPAIGVGVTMLLHCDLVVIAEEATFSTPFVGLGLVPEAGSSLLLPALIGMALANDMLLANRVLTAAEAHAAGLASRLVPGA-ELTAETARLAAGVA---ASAPTAMRETKALIR 210          
BLAST of mRNA_M-pyrifera_M_contig104979.1070.1 vs. uniprot
Match: A0A7S2UUF7_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2UUF7_9STRA)

HSP 1 Score: 100 bits (249), Expect = 1.590e-22
Identity = 51/107 (47.66%), Postives = 63/107 (58.88%), Query Frame = 1
Query:    1 APPNVFMRSVYLCNKLVVAAVNGHAVGAGVTVLPHCDLVVVVEGGKLWTPFVALGIVPELSSSTTFPSILGSARGKAALLAGRKLSSEELYHCGFASHILPPTTSFL 321
            +P   F  ++  C KLVVAAVNG AVG G T+L HCDLV +     LW PF  L +VPE  SS  FP I+G  R    L  G KL ++E   CGF + ILP T++ L
Sbjct:  112 SPAGRFKLAIMRCPKLVVAAVNGPAVGIGATLLAHCDLVFMSSEAYLWAPFFQLALVPEHCSSLLFPLIMGQGRAGRVLYLGEKLGAQEALDCGFITKILPSTSNIL 218          
BLAST of mRNA_M-pyrifera_M_contig104979.1070.1 vs. uniprot
Match: A0A840S1L2_9BURK (Enoyl-CoA hydratase/carnithine racemase n=1 Tax=Inhella inkyongensis TaxID=392593 RepID=A0A840S1L2_9BURK)

HSP 1 Score: 99.8 bits (247), Expect = 1.630e-22
Identity = 64/174 (36.78%), Postives = 90/174 (51.72%), Query Frame = 1
Query:    1 APPNVFMRSVYLCNKLVVAAVNGHAVGAGVTVLPHCDLVVVVEGGKLWTPFVALGIVPELSSSTTFPSILGSARGKAALLAGRKLSSEELYHCGFASHILPPTTSFLSNVLHLAASTAYATRDAPAAIKDAKVLIRSPHTLE---TAYKEYKILAKRVNEGMAMAAAMETIQRR 513
            AP   FM+++  C K V+AAVNG A+G G T+L HCDLV V +  +L  PFVALG+VPE  SS   P ++G AR    LL G  ++  E    G A+ +LP          H           APAA++ +K L+R+P   E      +E ++  +R+    AM A     Q+R
Sbjct:   77 APVFQFMQALLACEKPVIAAVNGAAIGIGTTMLLHCDLVYVADDARLAMPFVALGLVPEFGSSLVVPKLMGHARAAEKLLLGDPMTGAEALEFGIANAVLPAAEV----AAHARRMAERFNGLAPAAVRASKKLMRAPGLAELQRVIQEEAELFGQRLRSPEAMEAFQAFFQKR 246          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig104979.1070.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A5D6XXW0_9STRA5.370e-2638.73Uncharacterized protein n=1 Tax=Pythium brassicum ... [more]
UPI0019A0DAFB1.590e-2439.20Enoyl-CoA hydratase n=1 Tax=Chitinophagaceae bacte... [more]
UPI001F5385836.060e-2439.41enoyl-CoA hydratase-related protein n=2 Tax=Calidi... [more]
A0A7C9PJN2_9BURK2.990e-2337.14Enoyl-CoA hydratase n=1 Tax=Ideonella livida TaxID... [more]
A0A0N1L0Y5_9PROT3.370e-2337.36Enoyl-CoA hydratase n=3 Tax=Betaproteobacteria Tax... [more]
A0A835ZBJ2_9STRA7.210e-2350.00ClpP/crotonase-like domain-containing protein n=1 ... [more]
D0N4V4_PHYIT1.550e-2234.91Enoyl-CoA hydratase/isomerase family, putative n=1... [more]
UPI0013D6EB511.560e-2243.51enoyl-CoA hydratase-related protein n=1 Tax=Pseudo... [more]
A0A7S2UUF7_9STRA1.590e-2247.66Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
A0A840S1L2_9BURK1.630e-2236.78Enoyl-CoA hydratase/carnithine racemase n=1 Tax=In... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig104979contigM-pyrifera_M_contig104979:113..628 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score99.8
Seed ortholog evalue1.3e-18
Seed eggNOG ortholog65071.PYU1_T013079
Preferred nameECI1
KEGG rclassRC01078
KEGG koko:K13239
KEGG ReactionR04756
KEGG Pathwayko00071,ko04146,map00071,map04146
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0004165,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005777,GO:0005782,GO:0006082,GO:0006629,GO:0006631,GO:0006635,GO:0008150,GO:0008152,GO:0009056,GO:0009062,GO:0009987,GO:0016042,GO:0016054,GO:0016853,GO:0016860,GO:0016863,GO:0019395,GO:0019752,GO:0030258,GO:0031907,GO:0031974,GO:0032787,GO:0034440,GO:0042579,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043436,GO:0044237,GO:0044238,GO:0044242,GO:0044248,GO:0044255,GO:0044281,GO:0044282,GO:0044422,GO:0044424,GO:0044438,GO:0044439,GO:0044444,GO:0044446,GO:0044464,GO:0046395,GO:0055114,GO:0070013,GO:0071704,GO:0072329,GO:1901575
EggNOG free text desc.Enoyl-CoA hydratase isomerase family. Source PGD
EggNOG OGs1MCSZ@121069,COG1024@1,KOG0016@2759
EC5.3.3.8
COG Functional cat.I
BiGG ReactioniMM904.YOR180C
Best tax levelPythiales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Exons1
Model size516
Cds size498
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815557.8036678-CDS-M-pyrifera_M_contig104979:112..6101622815557.8036678-CDS-M-pyrifera_M_contig104979:112..610Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig104979 113..610 -
1692277462.9576952-CDS-M-pyrifera_M_contig104979:112..6101692277462.9576952-CDS-M-pyrifera_M_contig104979:112..610Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig104979 113..610 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815557.816608-UTR-M-pyrifera_M_contig104979:610..6281622815557.816608-UTR-M-pyrifera_M_contig104979:610..628Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig104979 611..628 -
1692277462.9703043-UTR-M-pyrifera_M_contig104979:610..6281692277462.9703043-UTR-M-pyrifera_M_contig104979:610..628Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig104979 611..628 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig104979.1070.1prot_M-pyrifera_M_contig104979.1070.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig104979 113..610 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig104979.1070.1

>prot_M-pyrifera_M_contig104979.1070.1 ID=prot_M-pyrifera_M_contig104979.1070.1|Name=mRNA_M-pyrifera_M_contig104979.1070.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=166bp
MRSVYLCNKLVVAAVNGHAVGAGVTVLPHCDLVVVVEGGKLWTPFVALGI
VPELSSSTTFPSILGSARGKAALLAGRKLSSEELYHCGFASHILPPTTSF
LSNVLHLAASTAYATRDAPAAIKDAKVLIRSPHTLETAYKEYKILAKRVN
EGMAMAAAMETIQRRK
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mRNA from alignment at M-pyrifera_M_contig104979:113..628-

Legend: UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig104979.1070.1 ID=mRNA_M-pyrifera_M_contig104979.1070.1|Name=mRNA_M-pyrifera_M_contig104979.1070.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=516bp|location=Sequence derived from alignment at M-pyrifera_M_contig104979:113..628- (Macrocystis pyrifera P11B4 male)
GCTCCACCCAATGTCTTTATGCGATCAGTATATCTATGCAACAAGTTGGT CGTTGCAGCTGTCAATGGGCATGCTGTGGGGGCAGGTGTGACAGTGTTAC CACACTGTGACTTGGTGGTGGTGGTGGAAGGAGGAAAGTTGTGGACTCCA TTTGTAGCTCTTGGAATTGTCCCTGAATTGAGTTCCTCCACAACGTTTCC ATCCATTTTGGGGAGTGCGAGGGGAAAAGCAGCACTTTTGGCAGGGAGGA AGTTGAGTAGTGAAGAATTGTACCATTGTGGATTTGCATCTCATATCCTT CCTCCCACAACCTCTTTTCTCTCCAATGTCCTCCACCTCGCGGCTTCCAC CGCCTATGCAACTAGAGATGCTCCAGCAGCCATCAAAGATGCAAAGGTAT TGATACGATCCCCACACACTCTCGAAACTGCCTACAAAGAGTACAAAATC CTTGCAAAGCGGGTGAATGAAGGAATGGCGATGGCAGCAGCGATGGAAAC AATCCAGAGAAGAAAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig104979:113..628-

>mRNA_M-pyrifera_M_contig104979.1070.1 ID=mRNA_M-pyrifera_M_contig104979.1070.1|Name=mRNA_M-pyrifera_M_contig104979.1070.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=996bp|location=Sequence derived from alignment at M-pyrifera_M_contig104979:113..628- (Macrocystis pyrifera P11B4 male)
ATGCGATCAGTATATCTATGCAACAAGTTGGTCGTTGCAGCTGTCAATGG
GCATGCTGTGGGGGCAGGTGTGACAGTGTTACCACACTGTGACTTGGTGG
TGGTGGTGGAAGGAGGAAAGTTGTGGACTCCATTTGTAGCTCTTGGAATT
GTCCCTGAATTGAGTTCCTCCACAACGTTTCCATCCATTTTGGGGAGTGC
GAGGGGAAAAGCAGCACTTTTGGCAGGGAGGAAGTTGAGTAGTGAAGAAT
TGTACCATTGTGGATTTGCATCTCATATCCTTCCTCCCACAACCTCTTTT
CTCTCCAATGTCCTCCACCTCGCGGCTTCCACCGCCTATGCAACTAGAGA
TGCTCCAGCAGCCATCAAAGATGCAAAGGTATTGATACGATCCCCACACA
CTCTCGAAACTGCCTACAAAGAGTACAAAATCCTTGCAAAGCGGGTGAAT
GAAGGAATGGCGATGGCAGCAGCGATGGAAACAATCCAGAGAAGAAAAAT
GCGATCAGTATATCTATGCAACAAGTTGGTCGTTGCAGCTGTCAATGGGC
ATGCTGTGGGGGCAGGTGTGACAGTGTTACCACACTGTGACTTGGTGGTG
GTGGTGGAAGGAGGAAAGTTGTGGACTCCATTTGTAGCTCTTGGAATTGT
CCCTGAATTGAGTTCCTCCACAACGTTTCCATCCATTTTGGGGAGTGCGA
GGGGAAAAGCAGCACTTTTGGCAGGGAGGAAGTTGAGTAGTGAAGAATTG
TACCATTGTGGATTTGCATCTCATATCCTTCCTCCCACAACCTCTTTTCT
CTCCAATGTCCTCCACCTCGCGGCTTCCACCGCCTATGCAACTAGAGATG
CTCCAGCAGCCATCAAAGATGCAAAGGTATTGATACGATCCCCACACACT
CTCGAAACTGCCTACAAAGAGTACAAAATCCTTGCAAAGCGGGTGAATGA
AGGAATGGCGATGGCAGCAGCGATGGAAACAATCCAGAGAAGAAAA
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