mRNA_M-pyrifera_M_contig104834.1033.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig104834.1033.1
Unique NamemRNA_M-pyrifera_M_contig104834.1033.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig104834.1033.1 vs. uniprot
Match: A0A3D2CF32_9DELT (Uncharacterized protein (Fragment) n=1 Tax=Deltaproteobacteria bacterium TaxID=2026735 RepID=A0A3D2CF32_9DELT)

HSP 1 Score: 116 bits (291), Expect = 1.790e-27
Identity = 67/168 (39.88%), Postives = 91/168 (54.17%), Query Frame = 1
Query:   16 AFRNAFSTTPWLLRYPDTDLGT-DYGMHDDSLTYSTLGKTSWYFWPSVEREGMTTTWKEHPMGGEIRPENQKNLFKASFGDDSNEQPLNDCTDTVHLSWALNAYAFDPGYTGIEEERAKKGAARMGYGYHLETLVYDWLPPNQTTLKLSATICNRGVAPSYYPLTASI 516
            A+ +AF++T   +R P  D  +   G HDDS  YST+G   W+F P +E       W E P+GGE+RPE Q  +F AS+ +   +Q  + C    H S+ LN +AF   Y   E+ RA++ A  MGY YHLE  V +       TL LS  I N GVAP Y PL  ++
Sbjct:  195 AYHDAFASTLLQVRIPTGDSPSLRIGFHDDSFAYSTVGDVPWFFVPLLEAAEADARWTEVPVGGELRPELQDQVFDASYEEGEFQQDFDACVQATHASFLLNFHAFSGIYDPEEQARARESARMMGYAYHLERAVLE-----AGTLTLS--IENHGVAPFYQPLQVTL 355          
BLAST of mRNA_M-pyrifera_M_contig104834.1033.1 vs. uniprot
Match: A0A2E9BAQ2_9ARCH (DUF4832 domain-containing protein n=1 Tax=Thermoplasmata archaeon TaxID=1906666 RepID=A0A2E9BAQ2_9ARCH)

HSP 1 Score: 105 bits (262), Expect = 2.590e-23
Identity = 61/165 (36.97%), Postives = 85/165 (51.52%), Query Frame = 1
Query:    7 LTDAFRNAFSTTPWLLRYPDTDL-GTDYGMHDDSLTYSTLGKTSWYFWPSVEREGMTTTWKEHPMGGEIRPENQKNLFKASFGDDSNEQPLNDCTDTVHLSWALNAYAFDPGY-TGIEEERAKKGAARMGYGYHLETLVYDWLPPNQTTLKLSATICNRGVAPSY 495
            + +A+ NAF+ T   +RYP  D      G HDDS  YST+G  +WYF PS+       +W+++P+GGEIRPE Q ++F  +      +Q  + C +T H S  LN  AF  G  T  E E A+  A  +GY  H+    +          +L   I NRGVAP Y
Sbjct:  202 ILNAYVNAFNQTLLQVRYPVVDSPNLRIGFHDDSFAYSTVGDVNWYFHPSLVAAEANESWRDNPIGGEIRPELQWDIFDENINISIEKQDFDLCVNTTHASMLLNYAAFGGGLNTTAERENAEAAALSLGYALHISNATF-------VGEQLEIYIENRGVAPFY 359          
BLAST of mRNA_M-pyrifera_M_contig104834.1033.1 vs. uniprot
Match: A0A5S5BN87_9BACL (Uncharacterized protein DUF4832 n=1 Tax=Paenibacillus methanolicus TaxID=582686 RepID=A0A5S5BN87_9BACL)

HSP 1 Score: 105 bits (261), Expect = 7.980e-23
Identity = 60/170 (35.29%), Postives = 87/170 (51.18%), Query Frame = 1
Query:    7 LTDAFRNAFSTTPWLLRYP-----DTDLGTDYGMHDDSLTYSTLGKTSWYFWPSVEREGMTTTWKEHPMGGEIRPENQKNLFKASFGDDSNEQPLNDCTDTVHLSWALNAYAFDPGYTGIEEERAKKGAARMGYGYHLETLVYDWLPPNQTTLKLSATICNRGVAPSYYP 501
            L +A+R+AFS T  LLR        T L   +G HDDS  YST+G  +W F PS++  G++  W+ H +GGE+RPE Q ++F    GD    Q      D  H+SW +N + FD    G E  ++      +GY  H+  +       N   +++   I N+G AP YYP
Sbjct:  211 LLEAYRDAFSHTQILLRNTTEGAASTSLLQQFGFHDDSFAYSTIGPETWQFAPSLQLAGLSNQWRTHAVGGELRPELQSSIFAG--GDQG--QGFQASVDATHISWMINNHVFDHEVAGSELTQSLNAHNSLGYQLHIPAVNIPRTIANGA-VQVGVQIQNKGNAPFYYP 375          
BLAST of mRNA_M-pyrifera_M_contig104834.1033.1 vs. uniprot
Match: UPI00037A1346 (DUF4832 domain-containing protein n=1 Tax=unclassified Leifsonia TaxID=2663824 RepID=UPI00037A1346)

HSP 1 Score: 98.2 bits (243), Expect = 2.020e-20
Identity = 62/179 (34.64%), Postives = 85/179 (47.49%), Query Frame = 1
Query:   19 FRNAFSTTPWLLRYPDT-DLGTDYGMHDDSLTYSTLGKTS---WYFWPSVEREGMTTTWKEHPMGGEIRPENQKNLFKAS---FGDDSNEQPLNDCTDTVHLSWALNAYAFDPGYTGIEEERAKKGAARMGYGYHLETLVYDWLPPNQTTLKLSATICNRGVAPSYY--PLTASIFSPS 528
            F NAF TT   +R P+T +     G HDDS    T  KTS   W+F  ++   G T  WK   +GGE+RPE Q  +F  S     +D  +          H+SW LN YAF  GY+  ++ RA  GA  MGY       +        ++  +  TI N G+AP YY  P+T ++   S
Sbjct:  217 FVNAFHTTKLEVRQPNTQNASLPIGYHDDSFALET--KTSSLGWHFMDNMAAAGATNKWKTQSIGGELRPELQSCIFSTSGCPVIEDGGDNDFPGSVSQTHVSWLLNQYAFQTGYSSSDKPRALAGAQSMGYSLRATEALVPATIATGSSASVGMTISNIGIAPFYYSWPITLALVDAS 393          
BLAST of mRNA_M-pyrifera_M_contig104834.1033.1 vs. uniprot
Match: A0A1I1XH40_9ACTN (DUF4832 domain-containing protein n=1 Tax=Streptomyces alni TaxID=380248 RepID=A0A1I1XH40_9ACTN)

HSP 1 Score: 95.5 bits (236), Expect = 1.440e-19
Identity = 58/165 (35.15%), Postives = 75/165 (45.45%), Query Frame = 1
Query:   19 FRNAFSTTPWLLRYPDTD-LGTDYGMHDDSLTYSTLGKTSWYFWPSVEREGMTTTWKEHPMGGEIRPENQKNLFKAS----FGDDSNEQPLNDCTDTVHLSWALNAYAFDPGYTGIEEERAKKGAARMGYGYHLETLVYDWLPPNQTTLKLSATICNRGVAPSYY 498
            F  AF TT    RYP TD      G HDDS    TL  T W+F       G T  W+  P+GGE+RPE Q  +F A+      +D  +          H SW L+ YAF PG+TG + +RA  GA  +GY   +             +L +   + N GVAP  Y
Sbjct:  243 FDAAFDTTRLQARYPSTDNAALGMGYHDDSFAVETLPGTGWHFMDKAIAAGTTDKWRTQPVGGELRPEIQGCIFDATPDCPVVEDGADNDFAASVAATHASWLLDQYAFAPGHTGADRDRALAGAQSLGYRLRVTAARTQ---AGGRSLTVGVRMENTGVAPFSY 404          
BLAST of mRNA_M-pyrifera_M_contig104834.1033.1 vs. uniprot
Match: UPI001E2D93D5 (DUF4832 domain-containing protein n=1 Tax=Kineosporia rhizophila TaxID=84633 RepID=UPI001E2D93D5)

HSP 1 Score: 93.2 bits (230), Expect = 5.350e-19
Identity = 60/176 (34.09%), Postives = 87/176 (49.43%), Query Frame = 1
Query:   13 DAFRNAFSTTPWLLRYPD-TDLGTDYGMHDDSLTYSTLGKTS-WYFWPSVEREGMTTTWKEHPMGGEIRPENQKNLFKASFGDDSNEQPLNDCTDTV---HLSWALNAYAFDPGYTGIEEERAKKGAARMGYGYHLETLVYDWLPPNQT---TLKLSATICNRGVAPSYYPLTASI 516
            +AF +AF TT   +R PD  +     G HDDS    T    + W+F   +   G+   WK   +GGE+RPE Q  +F  +          ND   +V   H+SW +N YAF  GY+  +  RA  GA  +GY +   T  Y  LP + T   ++ + A++ N G+AP YYP  A +
Sbjct:  134 NAFVSAFKTTELEVRNPDPVNADLPIGYHDDSFALETKPVAAGWHFMNKINDNGLQNKWKTQSIGGELRPELQSCIFSPAGCPVIEPDGDNDFPGSVQQTHISWMMNHYAFQTGYSTADRARALAGAKSLGYSFRA-TRAY--LPTSATAGSSITVGASVQNIGIAPFYYPWAARV 306          
BLAST of mRNA_M-pyrifera_M_contig104834.1033.1 vs. uniprot
Match: UPI0004006433 (DUF4832 domain-containing protein n=1 Tax=Hamadaea tsunoensis TaxID=53368 RepID=UPI0004006433)

HSP 1 Score: 93.6 bits (231), Expect = 6.230e-19
Identity = 59/169 (34.91%), Postives = 79/169 (46.75%), Query Frame = 1
Query:    7 LTDAFRNAFSTTPWLLRYPDTD-LGTDYGMHDDSLTYSTLGKTSWYFWPSVEREGMTTTWKEHPMGGEIRPENQKNLFKASFG----DDSNEQPLNDCTDTVHLSWALNAYAFDPGYTGIEEERAKKGAARMGYGYHLETLVYDWLPPNQTTLKLSATICNRGVAPSYY 498
            + +A+ +AF  T   +RYP  D      G HDDS    TL    W+F   + + G T  W    +GGE+RPE Q  +F A       +D  +          H+SW LN YAF PGYTG   +RA  GA  MGY     T V   +   +  L +   + + GVAP YY
Sbjct:  210 VVNAYTSAFRRTRLQVRYPGADNAAAPVGYHDDSFAVETLPGPGWHFMDKMTQAGATGKWLTQSIGGELRPEIQGCIFDAPADCPVIEDFADNDFPGGVAQTHVSWLLNQYAFAPGYTGGNYDRALAGAQSMGYRLQA-TGVRTTVDGRRHELGVDVRMQDLGVAPFYY 377          
BLAST of mRNA_M-pyrifera_M_contig104834.1033.1 vs. uniprot
Match: A0A5C5Z724_9BACT (DUF4832 domain-containing protein n=1 Tax=Planctomycetes bacterium CA13 TaxID=2527986 RepID=A0A5C5Z724_9BACT)

HSP 1 Score: 92.4 bits (228), Expect = 1.610e-18
Identity = 62/175 (35.43%), Postives = 82/175 (46.86%), Query Frame = 1
Query:   16 AFRNAFSTTPWLLRYPDTDLGTDY--------GMHDDSLTYSTL----GKTSWYFWPSVEREGMTTTWKEHPMGGEIRPENQKNLFKASFGDDS---NEQPLNDCTDTVHLSWALNAYAFDPGYTGIEEERAKKGAARMGYGYHLETLVYDWLPPNQTTLKLSATICNRGVAPSY 495
            A+  AF  TP LLRYP  +    Y        G HDDS  YST      K  +YF   + R  +T  WK+HP+GGEIRPE    ++   F   S    +Q    C D  H +W ++   F    +    E A K   RMGY +H+E+   D     +T + +   I NRGVAP Y
Sbjct:  204 AYAIAFKKTPILLRYPMGENDERYAPTKSYPFGYHDDSFAYSTRVTEQTKHDYYFMAQLTRAKLTNVWKQHPIGGEIRPE----VWGCVFDTPSCVPQDQSFELCVDETHATWLMDTGMFRKKASQDRYENAVKQVQRMGYEFHIESAKIDATKRAKTLVAIR--IRNRGVAPFY 372          
BLAST of mRNA_M-pyrifera_M_contig104834.1033.1 vs. uniprot
Match: A0A5C6E1H2_9BACT (DUF4832 domain-containing protein n=1 Tax=Novipirellula artificiosorum TaxID=2528016 RepID=A0A5C6E1H2_9BACT)

HSP 1 Score: 91.3 bits (225), Expect = 4.290e-18
Identity = 58/176 (32.95%), Postives = 82/176 (46.59%), Query Frame = 1
Query:   16 AFRNAFSTTPWLLRYPDTDLGTDY--------GMHDDSLTYSTL----GKTSWYFWPSVEREGMTTTWKEHPMGGEIRPENQKNLFKASFGDDS---NEQPLNDCTDTVHLSWALNAYAFDPGYTGIEEERAKKGAARMGYGYHLETLVYDWLPPNQTTLKLSATICNRGVAPSYY 498
            A+  AF TTP LLRYP  +    Y        G HDDS  ++T      +  W+F   + R  +   WK+HP+GGEIRPE    ++   F   S     Q   DC +  H +W ++   F         +RA +   RMGY +HL     D     +T  ++S  + NRGVAP Y+
Sbjct:  215 AYTKAFKTTPVLLRYPAGENDYHYAPTKHFPFGYHDDSFAWATRVTPRPEDDWFFIAKMNRADLMENWKQHPIGGEIRPE----VWGCVFDSPSCAPEGQAFADCVNETHATWLMDTGMFRETAAKPRYQRAIEQVGRMGYEFHLAAAKVDAAELQET--RVSVKVENRGVAPFYH 384          
BLAST of mRNA_M-pyrifera_M_contig104834.1033.1 vs. uniprot
Match: A0A7Z9G0Z9_9BACT (DUF4832 domain-containing protein n=2 Tax=Candidatus Poribacteria bacterium TaxID=2026781 RepID=A0A7Z9G0Z9_9BACT)

HSP 1 Score: 90.9 bits (224), Expect = 5.370e-18
Identity = 57/165 (34.55%), Postives = 77/165 (46.67%), Query Frame = 1
Query:   13 DAFRNAFSTTPWLLRYPDTDL-GTDYGMHDDSLTYSTLGKTSWYFWPSVEREGMTTTWKEHPMGGEIRPENQKNLFKASFGDDSNEQPLNDCTDTVHLSWALNAYAFDPGYTG-IEEERAKKGAARMGYGYHLE--TLVYDWLPPNQTTLKLSATICNRGVAPSY 495
            +A+  AF  T   +R P  D+     G HDDS  YSTLG   WYF   +        W+E P+GGE+RPE Q  +F+  +      Q  +DC +  H S  L A  +  G    IE ERA+  A  +GY  H+    +  D          L  ++ NRGVAP Y
Sbjct:  222 NAYATAFEKTFLQVRKPVADVTNLRIGFHDDSFAYSTLGDIDWYFHSLLIEAKADDRWREIPIGGELRPELQGEVFEDGYITGVYRQDFSDCVEATHASMLLAAQLYSGGLNSTIERERAEAAALSLGYALHVSRAAIFGD---------SLEVSLENRGVAPFY 377          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig104834.1033.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 20
Match NameE-valueIdentityDescription
A0A3D2CF32_9DELT1.790e-2739.88Uncharacterized protein (Fragment) n=1 Tax=Deltapr... [more]
A0A2E9BAQ2_9ARCH2.590e-2336.97DUF4832 domain-containing protein n=1 Tax=Thermopl... [more]
A0A5S5BN87_9BACL7.980e-2335.29Uncharacterized protein DUF4832 n=1 Tax=Paenibacil... [more]
UPI00037A13462.020e-2034.64DUF4832 domain-containing protein n=1 Tax=unclassi... [more]
A0A1I1XH40_9ACTN1.440e-1935.15DUF4832 domain-containing protein n=1 Tax=Streptom... [more]
UPI001E2D93D55.350e-1934.09DUF4832 domain-containing protein n=1 Tax=Kineospo... [more]
UPI00040064336.230e-1934.91DUF4832 domain-containing protein n=1 Tax=Hamadaea... [more]
A0A5C5Z724_9BACT1.610e-1835.43DUF4832 domain-containing protein n=1 Tax=Planctom... [more]
A0A5C6E1H2_9BACT4.290e-1832.95DUF4832 domain-containing protein n=1 Tax=Novipire... [more]
A0A7Z9G0Z9_9BACT5.370e-1834.55DUF4832 domain-containing protein n=2 Tax=Candidat... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig104834contigM-pyrifera_M_contig104834:1..573 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score109.8
Seed ortholog evalue1.5e-21
Seed eggNOG ortholog479435.Kfla_1513
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Domain of unknown function (DUF4832)
EggNOG OGs2GNR4@201174,4DTC4@85009,COG1874@1,COG1874@2
COG Functional cat.G
Best tax levelPropionibacteriales
Best eggNOG OGNA|NA|NA
Exons1
Model size573
Cds size573
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815555.8280113-CDS-M-pyrifera_M_contig104834:0..5731622815555.8280113-CDS-M-pyrifera_M_contig104834:0..573Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig104834 1..573 +
1692277462.0143442-CDS-M-pyrifera_M_contig104834:0..5731692277462.0143442-CDS-M-pyrifera_M_contig104834:0..573Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig104834 1..573 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig104834.1033.1prot_M-pyrifera_M_contig104834.1033.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig104834 1..573 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig104834.1033.1

>prot_M-pyrifera_M_contig104834.1033.1 ID=prot_M-pyrifera_M_contig104834.1033.1|Name=mRNA_M-pyrifera_M_contig104834.1033.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=191bp
QSLTDAFRNAFSTTPWLLRYPDTDLGTDYGMHDDSLTYSTLGKTSWYFWP
SVEREGMTTTWKEHPMGGEIRPENQKNLFKASFGDDSNEQPLNDCTDTVH
LSWALNAYAFDPGYTGIEEERAKKGAARMGYGYHLETLVYDWLPPNQTTL
KLSATICNRGVAPSYYPLTASIFSPSTNTTHDRISLHPLLP
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mRNA from alignment at M-pyrifera_M_contig104834:1..573+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig104834.1033.1 ID=mRNA_M-pyrifera_M_contig104834.1033.1|Name=mRNA_M-pyrifera_M_contig104834.1033.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=573bp|location=Sequence derived from alignment at M-pyrifera_M_contig104834:1..573+ (Macrocystis pyrifera P11B4 male)
CAATCTCTCACAGACGCTTTTCGAAACGCCTTTTCCACCACCCCATGGCT CTTGCGATATCCAGACACAGATTTGGGCACTGATTATGGGATGCATGATG ATTCGTTGACGTATTCAACACTTGGAAAGACTTCGTGGTACTTTTGGCCA TCTGTAGAACGCGAAGGAATGACAACTACTTGGAAGGAGCATCCGATGGG CGGAGAAATCCGGCCTGAGAATCAAAAGAATTTGTTCAAGGCTTCGTTTG GAGACGATTCCAACGAACAACCACTAAACGACTGCACTGACACAGTTCAC CTCTCCTGGGCATTGAATGCGTATGCTTTTGATCCAGGCTACACGGGAAT TGAAGAAGAGAGAGCAAAGAAAGGAGCTGCTCGCATGGGGTATGGGTATC ACCTTGAAACTCTTGTGTATGATTGGCTCCCACCAAATCAAACAACCCTA AAGTTGTCAGCAACGATTTGCAATCGTGGTGTTGCACCATCCTACTACCC TCTCACTGCCTCCATCTTTTCCCCTTCCACAAACACAACTCATGATCGCA TTTCTCTGCACCCGCTCCTCCCA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig104834:1..573+

>mRNA_M-pyrifera_M_contig104834.1033.1 ID=mRNA_M-pyrifera_M_contig104834.1033.1|Name=mRNA_M-pyrifera_M_contig104834.1033.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1146bp|location=Sequence derived from alignment at M-pyrifera_M_contig104834:1..573+ (Macrocystis pyrifera P11B4 male)
CAATCTCTCACAGACGCTTTTCGAAACGCCTTTTCCACCACCCCATGGCT
CTTGCGATATCCAGACACAGATTTGGGCACTGATTATGGGATGCATGATG
ATTCGTTGACGTATTCAACACTTGGAAAGACTTCGTGGTACTTTTGGCCA
TCTGTAGAACGCGAAGGAATGACAACTACTTGGAAGGAGCATCCGATGGG
CGGAGAAATCCGGCCTGAGAATCAAAAGAATTTGTTCAAGGCTTCGTTTG
GAGACGATTCCAACGAACAACCACTAAACGACTGCACTGACACAGTTCAC
CTCTCCTGGGCATTGAATGCGTATGCTTTTGATCCAGGCTACACGGGAAT
TGAAGAAGAGAGAGCAAAGAAAGGAGCTGCTCGCATGGGGTATGGGTATC
ACCTTGAAACTCTTGTGTATGATTGGCTCCCACCAAATCAAACAACCCTA
AAGTTGTCAGCAACGATTTGCAATCGTGGTGTTGCACCATCCTACTACCC
TCTCACTGCCTCCATCTTTTCCCCTTCCACAAACACAACTCATGATCGCA
TTTCTCTGCACCCGCTCCTCCCACAATCTCTCACAGACGCTTTTCGAAAC
GCCTTTTCCACCACCCCATGGCTCTTGCGATATCCAGACACAGATTTGGG
CACTGATTATGGGATGCATGATGATTCGTTGACGTATTCAACACTTGGAA
AGACTTCGTGGTACTTTTGGCCATCTGTAGAACGCGAAGGAATGACAACT
ACTTGGAAGGAGCATCCGATGGGCGGAGAAATCCGGCCTGAGAATCAAAA
GAATTTGTTCAAGGCTTCGTTTGGAGACGATTCCAACGAACAACCACTAA
ACGACTGCACTGACACAGTTCACCTCTCCTGGGCATTGAATGCGTATGCT
TTTGATCCAGGCTACACGGGAATTGAAGAAGAGAGAGCAAAGAAAGGAGC
TGCTCGCATGGGGTATGGGTATCACCTTGAAACTCTTGTGTATGATTGGC
TCCCACCAAATCAAACAACCCTAAAGTTGTCAGCAACGATTTGCAATCGT
GGTGTTGCACCATCCTACTACCCTCTCACTGCCTCCATCTTTTCCCCTTC
CACAAACACAACTCATGATCGCATTTCTCTGCACCCGCTCCTCCCA
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