mRNA_M-pyrifera_M_contig10481.1024.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig10481.1024.1
Unique NamemRNA_M-pyrifera_M_contig10481.1024.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig10481.1024.1 vs. uniprot
Match: D7FWF1_ECTSI (tRNA pseudouridine synthase B n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FWF1_ECTSI)

HSP 1 Score: 97.4 bits (241), Expect = 1.960e-24
Identity = 50/62 (80.65%), Postives = 54/62 (87.10%), Query Frame = 1 
Query:    1 VERDARPVTVYSLRQASPPPALPLVGLELECGGGFYVRTLIEDLARALGSRAHMTALERTRQ 186
            VER+AR V V SLRQA P   LPL+GLELECGGGFYVRTLIEDL RALG+RAHMTALERT+Q
Sbjct:   30 VEREARQVRVDSLRQADPALPLPLLGLELECGGGFYVRTLIEDLGRALGTRAHMTALERTKQ 91
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BLAST of mRNA_M-pyrifera_M_contig10481.1024.1 vs. uniprot
Match: A0A6H5KMC7_9PHAE (tRNA pseudouridine(55) synthase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KMC7_9PHAE)

HSP 1 Score: 97.4 bits (241), Expect = 8.830e-24
Identity = 50/62 (80.65%), Postives = 54/62 (87.10%), Query Frame = 1 
Query:    1 VERDARPVTVYSLRQASPPPALPLVGLELECGGGFYVRTLIEDLARALGSRAHMTALERTRQ 186
            VER+AR V V SLRQA P   LPL+GLELECGGGFYVRTLIEDL RALG+RAHMTALERT+Q
Sbjct:  108 VEREARQVRVDSLRQADPALPLPLLGLELECGGGFYVRTLIEDLGRALGTRAHMTALERTKQ 169
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BLAST of mRNA_M-pyrifera_M_contig10481.1024.1 vs. uniprot
Match: A0A835Z9D0_9STRA (tRNA pseudouridine(55) synthase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z9D0_9STRA)

HSP 1 Score: 77.0 bits (188), Expect = 1.850e-15
Identity = 37/62 (59.68%), Postives = 46/62 (74.19%), Query Frame = 1 
Query:    1 VERDARPVTVYSLRQASPPPALPLVGLELECGGGFYVRTLIEDLARALGSRAHMTALERTRQ 186
            VERDARPVT+Y L   +   ALP   L ++C GG Y+RTLI D+ARALG+R HMT+L RT+Q
Sbjct:  176 VERDARPVTIYQLEAPAEGVALPSFALNVQCSGGTYIRTLIADIARALGTRGHMTSLVRTKQ 237
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BLAST of mRNA_M-pyrifera_M_contig10481.1024.1 vs. uniprot
Match: A0A8C6LJI8_NOTFU (tRNA pseudouridine(55) synthase n=2 Tax=Nothobranchius furzeri TaxID=105023 RepID=A0A8C6LJI8_NOTFU)

HSP 1 Score: 65.5 bits (158), Expect = 1.170e-11
Identity = 35/62 (56.45%), Postives = 44/62 (70.97%), Query Frame = 1 
Query:    1 VERDARPVTVYSLRQASPPPALPLVGLELECGGGFYVRTLIEDLARALGSRAHMTALERTRQ 186
            V + ARPVTVYSL      P  PL  L++ECGGGFYVR+L++DL +AL S AH+  L RT+Q
Sbjct:   77 VAKPARPVTVYSLTLQEFKP--PLFTLDIECGGGFYVRSLVDDLGKALSSCAHVKELIRTKQ 136
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BLAST of mRNA_M-pyrifera_M_contig10481.1024.1 vs. uniprot
Match: A0A8J2SXF4_9STRA (tRNA pseudouridine(55) synthase n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2SXF4_9STRA)

HSP 1 Score: 66.6 bits (161), Expect = 1.580e-11
Identity = 37/70 (52.86%), Postives = 44/70 (62.86%), Query Frame = 1 
Query:    1 VERDARPVTVYSLRQASP--------PPALPLVGLELECGGGFYVRTLIEDLARALGSRAHMTALERTRQ 186
            VER+AR V+V  LR            P +LP  GL++EC GG Y+RTLI+DLAR  GS  HM  LERTRQ
Sbjct:  186 VEREARQVSVSRLRMVDDRGVGSHEAPLSLPFFGLDVECSGGTYIRTLIDDLAREAGSCGHMVELERTRQ 255
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BLAST of mRNA_M-pyrifera_M_contig10481.1024.1 vs. uniprot
Match: A0A7K2AJG5_9ACTN (tRNA pseudouridine synthase B n=1 Tax=Acidimicrobiia bacterium TaxID=2080302 RepID=A0A7K2AJG5_9ACTN)

HSP 1 Score: 66.6 bits (161), Expect = 1.910e-11
Identity = 36/62 (58.06%), Postives = 43/62 (69.35%), Query Frame = 1 
Query:    1 VERDARPVTVYSLRQAS-PPPALPLVGLELECGGGFYVRTLIEDLARALGSRAHMTALERTR 183
            VERDARP TV+ L+       + PLVGL + CG G YVR+L +DLARALG RAH+  L RTR
Sbjct:  164 VERDARPATVHFLKVTGFEKGSNPLVGLRVVCGKGLYVRSLADDLARALGGRAHLVELRRTR 225
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BLAST of mRNA_M-pyrifera_M_contig10481.1024.1 vs. uniprot
Match: UPI00198CCE54 (probable tRNA pseudouridine synthase 1 n=1 Tax=Silurus meridionalis TaxID=175797 RepID=UPI00198CCE54)

HSP 1 Score: 65.9 bits (159), Expect = 3.300e-11
Identity = 35/60 (58.33%), Postives = 43/60 (71.67%), Query Frame = 1 
Query:    7 RDARPVTVYSLRQASPPPALPLVGLELECGGGFYVRTLIEDLARALGSRAHMTALERTRQ 186
            + ARPVTVYSL      P  PL  LE+ECGGGFYVR+L++DL +AL S AH+  L RT+Q
Sbjct:  170 KPARPVTVYSLSLQDFNP--PLFTLEIECGGGFYVRSLVDDLGKALSSCAHVQELTRTKQ 227
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BLAST of mRNA_M-pyrifera_M_contig10481.1024.1 vs. uniprot
Match: A0A4W4EJJ6_ELEEL (tRNA pseudouridine(55) synthase n=2 Tax=Electrophorus electricus TaxID=8005 RepID=A0A4W4EJJ6_ELEEL)

HSP 1 Score: 65.9 bits (159), Expect = 3.430e-11
Identity = 34/60 (56.67%), Postives = 43/60 (71.67%), Query Frame = 1 
Query:    7 RDARPVTVYSLRQASPPPALPLVGLELECGGGFYVRTLIEDLARALGSRAHMTALERTRQ 186
            + ARPVTVYSL      P  PL  L++ECGGGFYVR+L++DL +AL S AH+  L RT+Q
Sbjct:  180 KPARPVTVYSLSLQDFSP--PLFSLDIECGGGFYVRSLVDDLGKALSSCAHVRELTRTKQ 237
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BLAST of mRNA_M-pyrifera_M_contig10481.1024.1 vs. uniprot
Match: A0A1A8B795_NOTFU (tRNA pseudouridine(55) synthase n=11 Tax=Nothobranchiidae TaxID=405002 RepID=A0A1A8B795_NOTFU)

HSP 1 Score: 65.5 bits (158), Expect = 3.720e-11
Identity = 35/62 (56.45%), Postives = 44/62 (70.97%), Query Frame = 1 
Query:    1 VERDARPVTVYSLRQASPPPALPLVGLELECGGGFYVRTLIEDLARALGSRAHMTALERTRQ 186
            V + ARPVTVYSL      P  PL  L++ECGGGFYVR+L++DL +AL S AH+  L RT+Q
Sbjct:  169 VAKPARPVTVYSLTLQEFKP--PLFTLDIECGGGFYVRSLVDDLGKALSSCAHVKELIRTKQ 228
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BLAST of mRNA_M-pyrifera_M_contig10481.1024.1 vs. uniprot
Match: UPI0006AAFFE4 (probable tRNA pseudouridine synthase 1 isoform X2 n=1 Tax=Pundamilia nyererei TaxID=303518 RepID=UPI0006AAFFE4)

HSP 1 Score: 64.7 bits (156), Expect = 4.530e-11
Identity = 34/60 (56.67%), Postives = 43/60 (71.67%), Query Frame = 1 
Query:    7 RDARPVTVYSLRQASPPPALPLVGLELECGGGFYVRTLIEDLARALGSRAHMTALERTRQ 186
            + ARPVTVYSL      P  PL  L++ECGGGFYVR+L++DL +AL S AH+  L RT+Q
Sbjct:  125 KPARPVTVYSLTLQEFKP--PLFTLDIECGGGFYVRSLVDDLGKALSSCAHVKELVRTKQ 182
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig10481.1024.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FWF1_ECTSI1.960e-2480.65tRNA pseudouridine synthase B n=1 Tax=Ectocarpus s... [more]
A0A6H5KMC7_9PHAE8.830e-2480.65tRNA pseudouridine(55) synthase n=1 Tax=Ectocarpus... [more]
A0A835Z9D0_9STRA1.850e-1559.68tRNA pseudouridine(55) synthase n=1 Tax=Tribonema ... [more]
A0A8C6LJI8_NOTFU1.170e-1156.45tRNA pseudouridine(55) synthase n=2 Tax=Nothobranc... [more]
A0A8J2SXF4_9STRA1.580e-1152.86tRNA pseudouridine(55) synthase n=1 Tax=Pelagomona... [more]
A0A7K2AJG5_9ACTN1.910e-1158.06tRNA pseudouridine synthase B n=1 Tax=Acidimicrobi... [more]
UPI00198CCE543.300e-1158.33probable tRNA pseudouridine synthase 1 n=1 Tax=Sil... [more]
A0A4W4EJJ6_ELEEL3.430e-1156.67tRNA pseudouridine(55) synthase n=2 Tax=Electropho... [more]
A0A1A8B795_NOTFU3.720e-1156.45tRNA pseudouridine(55) synthase n=11 Tax=Nothobran... [more]
UPI0006AAFFE44.530e-1156.67probable tRNA pseudouridine synthase 1 isoform X2 ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig10481contigM-pyrifera_M_contig10481:86..271 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score100.1
Seed ortholog evalue3.7e-19
Seed eggNOG ortholog2880.D7FWF1
Preferred nameTRUB1
KEGG koko:K03177
Hectar predicted targeting categoryother localisation
GOsGO:0000049,GO:0001522,GO:0003674,GO:0003676,GO:0003723,GO:0003824,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005739,GO:0005829,GO:0006139,GO:0006396,GO:0006399,GO:0006400,GO:0006725,GO:0006807,GO:0008033,GO:0008150,GO:0008152,GO:0009451,GO:0009982,GO:0009987,GO:0010467,GO:0016070,GO:0016071,GO:0016556,GO:0016853,GO:0016866,GO:0031119,GO:0034337,GO:0034470,GO:0034641,GO:0034660,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043412,GO:0044237,GO:0044238,GO:0044424,GO:0044444,GO:0044464,GO:0046483,GO:0071704,GO:0090304,GO:0097159,GO:1901360,GO:1901363,GO:1990481
EggNOG free text desc.mRNA pseudouridine synthesis
EggNOG OGsCOG0130@1,KOG2529@2759
EC5.4.99.25
COG Functional cat.J
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko03016
Exons1
Model size186
Cds size186
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815555.423626-CDS-M-pyrifera_M_contig10481:85..2711622815555.423626-CDS-M-pyrifera_M_contig10481:85..271Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig10481 86..271 -
1692277461.8769572-CDS-M-pyrifera_M_contig10481:85..2711692277461.8769572-CDS-M-pyrifera_M_contig10481:85..271Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig10481 86..271 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig10481.1024.1prot_M-pyrifera_M_contig10481.1024.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig10481 86..271 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig10481.1024.1

>prot_M-pyrifera_M_contig10481.1024.1 ID=prot_M-pyrifera_M_contig10481.1024.1|Name=mRNA_M-pyrifera_M_contig10481.1024.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=62bp
VERDARPVTVYSLRQASPPPALPLVGLELECGGGFYVRTLIEDLARALGS
RAHMTALERTRQ
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mRNA from alignment at M-pyrifera_M_contig10481:86..271-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig10481.1024.1 ID=mRNA_M-pyrifera_M_contig10481.1024.1|Name=mRNA_M-pyrifera_M_contig10481.1024.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=186bp|location=Sequence derived from alignment at M-pyrifera_M_contig10481:86..271- (Macrocystis pyrifera P11B4 male)
GTGGAGCGAGACGCTCGACCTGTAACTGTTTACTCCCTCCGGCAGGCAAG
CCCGCCCCCGGCGCTGCCTCTGGTGGGGCTCGAGCTGGAGTGCGGCGGGG
GGTTTTACGTGCGCACCCTCATCGAGGACCTCGCCAGGGCTCTTGGCTCG
AGGGCCCACATGACGGCGCTCGAGAGGACCAGGCAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig10481:86..271-

>mRNA_M-pyrifera_M_contig10481.1024.1 ID=mRNA_M-pyrifera_M_contig10481.1024.1|Name=mRNA_M-pyrifera_M_contig10481.1024.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=372bp|location=Sequence derived from alignment at M-pyrifera_M_contig10481:86..271- (Macrocystis pyrifera P11B4 male)
GTGGAGCGAGACGCTCGACCTGTAACTGTTTACTCCCTCCGGCAGGCAAG
CCCGCCCCCGGCGCTGCCTCTGGTGGGGCTCGAGCTGGAGTGCGGCGGGG
GGTTTTACGTGCGCACCCTCATCGAGGACCTCGCCAGGGCTCTTGGCTCG
AGGGCCCACATGACGGCGCTCGAGAGGACCAGGCAGGTGGAGCGAGACGC
TCGACCTGTAACTGTTTACTCCCTCCGGCAGGCAAGCCCGCCCCCGGCGC
TGCCTCTGGTGGGGCTCGAGCTGGAGTGCGGCGGGGGGTTTTACGTGCGC
ACCCTCATCGAGGACCTCGCCAGGGCTCTTGGCTCGAGGGCCCACATGAC
GGCGCTCGAGAGGACCAGGCAG
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