mRNA_M-pyrifera_M_contig104107.884.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig104107.884.1
Unique NamemRNA_M-pyrifera_M_contig104107.884.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig104107.884.1 vs. uniprot
Match: A0A2E0RX50_9ACTN (Fe/B12 periplasmic-binding domain-containing protein n=1 Tax=Acidimicrobiaceae bacterium TaxID=2024894 RepID=A0A2E0RX50_9ACTN)

HSP 1 Score: 184 bits (466), Expect = 1.040e-53
Identity = 95/187 (50.80%), Postives = 129/187 (68.98%), Query Frame = 1
Query:    1 DQVGEELLSGIG-EVVVVPDNLDADEQLTVLGELFEREAEAAALISELEAAQLSADEAISDECVVSLATIYGGPSVAAYVEPAWPLPTAIVDAGCTLDPDTSDADPDSNGRAWLSLEQLGLLDAPTLVLFQTSSVEGEDEAIEVVESQPLWPTLPAVASDQIIVFDRLGYPGAAGQIRFLGELEAAL 558
            DQVGE+ L+G+G EV V+PD  +  E++ +LG  F REA+A ALI++L+AA   A+    D+C VS+A IY G +VAA+ EP W  P A+   GCTL P   + D D NGRA+LSLEQLGLL APT+VL Q  +V+G+ EA+  + + P+W  LPAVA+ Q++  DR+GYPG  G+IR + EL  AL
Sbjct:  179 DQVGEKALAGVGAEVHVLPDGTEGPERIALLGGFFGREAQADALIADLDAAYARAEGEAPDDCAVSVAAIYPGANVAAFAEPVWDAPRALARLGCTLVPSGDNVDTDRNGRAYLSLEQLGLLSAPTMVLLQAPAVDGDSEAVAEITANPIWQQLPAVAAGQVVELDRIGYPGVVGEIRLIDELVGAL 365          
BLAST of mRNA_M-pyrifera_M_contig104107.884.1 vs. uniprot
Match: A0A4R7I2U5_9ACTN (Iron complex transport system substrate-binding protein n=1 Tax=Ilumatobacter fluminis TaxID=467091 RepID=A0A4R7I2U5_9ACTN)

HSP 1 Score: 177 bits (450), Expect = 1.120e-51
Identity = 91/187 (48.66%), Postives = 128/187 (68.45%), Query Frame = 1
Query:    1 DQVGEELLSGIG-EVVVVPDNLDADEQLTVLGELFEREAEAAALISELEAAQLSADEAISDECVVSLATIYGGPSVAAYVEPAWPLPTAIVDAGCTLDPDTSDADPDSNGRAWLSLEQLGLLDAPTLVLFQTSSVEGEDEAIEVVESQPLWPTLPAVASDQIIVFDRLGYPGAAGQIRFLGELEAAL 558
            +QVG E L+G+G EV V+PD     EQ  +LGE F R+A+A  L++EL+A   ++   + D C VS+A IY G +VA + EP W  P+A+ + GC L P   + D D NGR +LSLEQLGLL APT++L QT +V+GE E++  +E+ PLW TLPAV +D++ V DR+GYPG AG+IR + +L  A+
Sbjct:  146 NQVGAEQLAGVGAEVHVLPDGSTTREQAILLGEFFGRQAQAEELVAELDAEYAASQGEVPDGCEVSVAAIYAGANVAVFAEPIWTAPSAVTELGCQLVPRGDEVDTDRNGRLYLSLEQLGLLSAPTMILLQTEAVDGEAESVAEIEANPLWQTLPAVQADRVTVLDRIGYPGIAGEIRLIDDLIVAV 332          
BLAST of mRNA_M-pyrifera_M_contig104107.884.1 vs. uniprot
Match: A0A6C7EJB4_ILUCY (Fe/B12 periplasmic-binding domain-containing protein n=1 Tax=Ilumatobacter coccineus (strain NBRC 103263 / KCTC 29153 / YM16-304) TaxID=1313172 RepID=A0A6C7EJB4_ILUCY)

HSP 1 Score: 172 bits (435), Expect = 3.670e-49
Identity = 91/187 (48.66%), Postives = 118/187 (63.10%), Query Frame = 1
Query:    1 DQVGEELLSGIG-EVVVVPDNLDADEQLTVLGELFEREAEAAALISELEAAQLSADEAISDECVVSLATIYGGPSVAAYVEPAWPLPTAIVDAGCTLDPDTSDADPDSNGRAWLSLEQLGLLDAPTLVLFQTSSVEGEDEAIEVVESQPLWPTLPAVASDQIIVFDRLGYPGAAGQIRFLGELEAAL 558
            D+VG E L+G+G E+ V+P      EQL +LGE F+R   A+ +I+EL+AA   A   ++D+C  S+  IY GP+VAA+  P W +P AI + GC L P      PD NGR WLSLEQLGLLD P L++ QT +VEGE  AI+ V   PLW  LPAV S  +   DRLGY G  G+IR L ++ A L
Sbjct:  172 DEVGAEQLAGVGSELHVIPSGSSGREQLELLGEWFDRTDRASEMIAELDAAYDDARSRVADDCAASVVAIYAGPNVAAFAGPVWDVPRAIDELGCELVPSADGLSPDGNGRVWLSLEQLGLLDGPELIMLQTDTVEGEQAAIDDVTGSPLWSQLPAVQSGDVTTLDRLGYAGIDGEIRLLDDIVAIL 358          
BLAST of mRNA_M-pyrifera_M_contig104107.884.1 vs. uniprot
Match: UPI00130E8869 (ABC transporter substrate-binding protein n=1 Tax=Ilumatobacter nonamiensis TaxID=467093 RepID=UPI00130E8869)

HSP 1 Score: 164 bits (416), Expect = 2.440e-46
Identity = 86/185 (46.49%), Postives = 117/185 (63.24%), Query Frame = 1
Query:    1 DQVGEELLSGIG-EVVVVPDNLDADEQLTVLGELFEREAEAAALISELEAAQLSADEAISDECVVSLATIYGGPSVAAYVEPAWPLPTAIVDAGCTLDPDTSDADPDSNGRAWLSLEQLGLLDAPTLVLFQTSSVEGEDEAIEVVESQPLWPTLPAVASDQIIVFDRLGYPGAAGQIRFLGELEA 552
            DQVG E L G+G EV+V+PD   + E++ + GE F REAEA  LI++L+AA     E + ++C  S+  IY GPS A +  P W +P A+   GC L P   + + D NGRA+LSLEQL ++  P L+L Q  +V G+ EA+E +E  P+W  LPAVAS  + V DRLGYPG  G+ R + EL A
Sbjct:  170 DQVGREQLDGLGAEVLVIPDGTTSRERVELFGEHFGREAEAEGLIADLDAAYAVGSEQVPEDCAASVVAIYPGPSPAVFARPVWAVPLAVDTLGCELVPSGDEIETDGNGRAYLSLEQLEVIAGPELILLQNDTVTGDTEAVEEIEQNPIWGQLPAVASGDVTVIDRLGYPGLVGETRVVDELVA 354          
BLAST of mRNA_M-pyrifera_M_contig104107.884.1 vs. uniprot
Match: A0A2E4W1K6_9ACTN (Fe/B12 periplasmic-binding domain-containing protein n=1 Tax=Actinomycetia bacterium TaxID=1883427 RepID=A0A2E4W1K6_9ACTN)

HSP 1 Score: 119 bits (298), Expect = 4.480e-29
Identity = 68/184 (36.96%), Postives = 110/184 (59.78%), Query Frame = 1
Query:    7 VGEELLSGIGEVVVVPDNLDADEQLTVLGELFEREAEAAALISELEAAQLSADEAISDECVVSLATIYGGPSVAAYVEPAWPLPTAIVDAGCTLDPDTSDADPDSNGRAWLSLEQLGLLDAPTLVLFQTSSVEGEDEAIEVVESQPLWPTLPAVASDQIIVFDRLGYPGAAGQIRFLGELEAAL 558
            V ++LL GI    V+       E++  LGE+    ++A AL+++ + A  +A   +S +  VSLATIY G ++A +V+    +P  I+D G  L P  +      NGRA++S+E++G L +P ++L Q+++VEGEDE++  V+   LW  LPAV S ++   DRLGYPG  G+IR + +L   +
Sbjct:  155 VDQDLLDGIAPTSVISGE-GWRERVMSLGEILNAPSQADALLADYDLAISNAKAELSGDISVSLATIYPGSNIAVWVDGPSNIPQTILDMGIELSPGINSYSSAMNGRAYISIEKIGDLSSPIIILSQSTAVEGEDESLRNVQDNSLWKNLPAVTSGKVSTIDRLGYPGVEGRIRLINDLVGTI 337          
BLAST of mRNA_M-pyrifera_M_contig104107.884.1 vs. uniprot
Match: A0A2D6UWJ2_9ACTN (Fe/B12 periplasmic-binding domain-containing protein n=1 Tax=Acidimicrobiaceae bacterium TaxID=2024894 RepID=A0A2D6UWJ2_9ACTN)

HSP 1 Score: 110 bits (274), Expect = 2.050e-25
Identity = 63/186 (33.87%), Postives = 107/186 (57.53%), Query Frame = 1
Query:    1 DQVGEELLSGIGEVVVVPDNLDADEQLTVLGELFEREAEAAALISELEAAQLSADEAISDECVVSLATIYGGPSVAAYVEPAWPLPTAIVDAGCTLDPDTSDADPDSNGRAWLSLEQLGLLDAPTLVLFQTSSVEGEDEAIEVVESQPLWPTLPAVASDQIIVFDRLGYPGAAGQIRFLGELEAAL 558
            D V  ++L GI    VV  + D  +Q+  LGE+      A  +++E +       + ++ +  VS+ TIY G ++A +V+    +P  ++D G  L P+ +       GRA+LSLEQ+  L++P ++L Q++ V GE+E++  +E+ PLW  LPAV +  +IVF+RLGYPG  G++ F+ +    L
Sbjct:  178 DIVSLDILEGITTTKVVTSS-DWRQQVIELGEILGTNDTADEILNEYDTLISETKQLLASDLTVSVVTIYPGNTIAVWVDGPSNIPQTLIDLGVKLSPNKNTYQNARYGRAYLSLEQISELNSPFILLSQSNEVSGENESLSEIENNPLWQNLPAVKNGDVIVFNRLGYPGVEGRMEFIKDFREGL 362          
BLAST of mRNA_M-pyrifera_M_contig104107.884.1 vs. uniprot
Match: A0A178LSY8_9CHLR (ABC transporter substrate-binding protein n=1 Tax=Chloroflexus islandicus TaxID=1707952 RepID=A0A178LSY8_9CHLR)

HSP 1 Score: 105 bits (261), Expect = 7.560e-24
Identity = 67/189 (35.45%), Postives = 106/189 (56.08%), Query Frame = 1
Query:    1 DQVGEELLSGIGEVVVVPDNLDADEQLTVLGELFEREAEAAALISELEA-AQLSADEAISDECVVSLATIYGGPSVAAYVEPAWPLPTAIVDAGCTLDPDTSDADPDS--NGRAWLSLEQLGLLDAPTLVLFQTSSVEGEDEAIEVVESQPLWPTLPAVASDQIIVFDRLGYPGAAGQIRFLGELEAAL 558
            +Q+G E LS I   + +      +  +  L  +F R  EA   ++   A  Q+ A+   +    VS+ TIY GP+VA + +   P+P  + + G    PD +  +  +  NGRA++SLEQL L    T++L QTS VEGE +A+  +++ PLW  LPAV + ++++ DR+GYPG  GQ   L +L+ AL
Sbjct:  128 EQIGYERLSKIAPTIALSGATPLENYVETLA-VFGRGEEAQRTVANFRAEVQVMANRLNAAGQSVSMMTIYPGPNVALWFDGPSPIPLLVRELGIQFRPDPATVNGLNIRNGRAFISLEQLTLASGETILLLQTSGVEGEAQAVVELQANPLWQQLPAVQAQRVVILDRIGYPGLRGQRALLADLQQAL 315          
BLAST of mRNA_M-pyrifera_M_contig104107.884.1 vs. uniprot
Match: A0A2E3WQY7_9ACTN (Fe/B12 periplasmic-binding domain-containing protein n=1 Tax=Acidimicrobiaceae bacterium TaxID=2024894 RepID=A0A2E3WQY7_9ACTN)

HSP 1 Score: 105 bits (262), Expect = 9.510e-24
Identity = 65/181 (35.91%), Postives = 106/181 (58.56%), Query Frame = 1
Query:   16 ELLSGIGEVVVVPDNLDADEQLTVLGELFEREAEAAALISELEAAQLSADEAISDECVVSLATIYGGPSVAAYVEPAWPLPTAIVDAGCTLDPDTSDADPDSNGRAWLSLEQLGLLDAPTLVLFQTSSVEGEDEAIEVVESQPLWPTLPAVASDQIIVFDRLGYPGAAGQIRFLGELEAAL 558
            +LL+ I    V+  + D  +Q+  LG++ +    A  L++E ++    +   I++   VSLATIY G ++AA+V     +P  ++D G TL P+ +       GR +LS+EQL    +P +VL Q+++VEGED+++  +E   LW  LPAV S +++  DRLGYPG  G+IR + +  A L
Sbjct:  172 DLLTEIASTEVLTSD-DWRQQVIELGQVLDTPQRAEDLLTEYDSVIAESKIDITEGMEVSLATIYSGITIAAWVSGPSNIPQTLLDLGVTLYPNETTYPNVRYGRTYLSIEQLSDFSSPIIVLSQSTAVEGEDQSLIDIEKNLLWKNLPAVKSGKVLTIDRLGYPGVEGRIRLIKDFLAGL 351          
BLAST of mRNA_M-pyrifera_M_contig104107.884.1 vs. uniprot
Match: UPI0019F7CF49 (ABC transporter substrate-binding protein n=1 Tax=Chloroflexi bacterium AL-N15 TaxID=2686370 RepID=UPI0019F7CF49)

HSP 1 Score: 101 bits (252), Expect = 1.780e-22
Identity = 63/164 (38.41%), Postives = 94/164 (57.32%), Query Frame = 1
Query:   73 EQLTVLGELFEREAEAAALISELEAAQLSADEAISDECVVSLATIYGGPSVAAYVEPAWPLPTAIVDAGCTLDPD--TSDADPDSNGRAWLSLEQLGLLDAPTLVLFQTSSVEGEDEAIEVVESQPLWPTLPAVASDQIIVFDRLGYPGAAGQIRFLGELEAAL 558
            E LTV G+    +    A  +++EA     D A      VS+ATIY   +VA + +   P+P  + + G    PD  T D     NGRA++SLEQ+ L+   TL L Q+S VEGE++A++ +++  LW  LP+V +DQ+I  DR+GYPG  GQ + L ++  AL
Sbjct:  159 ETLTVFGQSEAAQRTIDAFRAQIEAESERVDAANQQ---VSVATIYSAENVALWFDGPSPIPRLLHELGVQFQPDPATIDGLDIRNGRAFISLEQIPLMSGDTLFLLQSSKVEGENDAVDAIQTTALWQQLPSVQTDQVIRLDRIGYPGLRGQYQLLDDVVQAL 319          
BLAST of mRNA_M-pyrifera_M_contig104107.884.1 vs. uniprot
Match: A0A2E9LBL8_9ACTN (Fe/B12 periplasmic-binding domain-containing protein n=1 Tax=Acidimicrobiaceae bacterium TaxID=2024894 RepID=A0A2E9LBL8_9ACTN)

HSP 1 Score: 101 bits (251), Expect = 4.080e-22
Identity = 56/181 (30.94%), Postives = 103/181 (56.91%), Query Frame = 1
Query:   16 ELLSGIGEVVVVPDNLDADEQLTVLGELFEREAEAAALISELEAAQLSADEAISDECVVSLATIYGGPSVAAYVEPAWPLPTAIVDAGCTLDPDTSDADPDSNGRAWLSLEQLGLLDAPTLVLFQTSSVEGEDEAIEVVESQPLWPTLPAVASDQIIVFDRLGYPGAAGQIRFLGELEAAL 558
            ++L  I    VV  + D  +++  LGE+FE    A  +++E +       ++++ +  VS+ T+Y G ++A +V+    +P  ++D G  L P+         GRA+LSLEQ+  L++P ++L Q++ V GE ++++ +E   LW  LPAV S  +++ +RLGYPG  G++R + +    L
Sbjct:  183 DMLEEIATTKVVT-STDWRQRVIELGEIFETSNIADEILNEYDTLISETKQSLASDLTVSVVTVYPGNTIAVWVDGPSNIPQTLIDLGVELFPNKDTYQNVRYGRAYLSLEQISDLNSPLIILSQSNEVSGESDSLDEIEKNSLWQNLPAVKSGNVMIINRLGYPGVEGRMRLIKDFREGL 362          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig104107.884.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 11
Match NameE-valueIdentityDescription
A0A2E0RX50_9ACTN1.040e-5350.80Fe/B12 periplasmic-binding domain-containing prote... [more]
A0A4R7I2U5_9ACTN1.120e-5148.66Iron complex transport system substrate-binding pr... [more]
A0A6C7EJB4_ILUCY3.670e-4948.66Fe/B12 periplasmic-binding domain-containing prote... [more]
UPI00130E88692.440e-4646.49ABC transporter substrate-binding protein n=1 Tax=... [more]
A0A2E4W1K6_9ACTN4.480e-2936.96Fe/B12 periplasmic-binding domain-containing prote... [more]
A0A2D6UWJ2_9ACTN2.050e-2533.87Fe/B12 periplasmic-binding domain-containing prote... [more]
A0A178LSY8_9CHLR7.560e-2435.45ABC transporter substrate-binding protein n=1 Tax=... [more]
A0A2E3WQY7_9ACTN9.510e-2435.91Fe/B12 periplasmic-binding domain-containing prote... [more]
UPI0019F7CF491.780e-2238.41ABC transporter substrate-binding protein n=1 Tax=... [more]
A0A2E9LBL8_9ACTN4.080e-2230.94Fe/B12 periplasmic-binding domain-containing prote... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig104107contigM-pyrifera_M_contig104107:3..566 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score177.9
Seed ortholog evalue4.3e-42
Seed eggNOG ortholog1313172.YM304_37520
KEGG koko:K02016
KEGG TC3.A.1.14
KEGG Pathwayko02010,map02010
KEGG ModuleM00240
Hectar predicted targeting categoryother localisation
EggNOG free text desc.abc-type fe3 -hydroxamate transport system, periplasmic component
EggNOG OGs2HBYV@201174,COG0614@1,COG0614@2
COG Functional cat.P
Best tax levelActinobacteria
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko02000
Exons1
Model size564
Cds size564
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815547.8208-CDS-M-pyrifera_M_contig104107:2..5661622815547.8208-CDS-M-pyrifera_M_contig104107:2..566Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig104107 3..566 +
1692277458.553851-CDS-M-pyrifera_M_contig104107:2..5661692277458.553851-CDS-M-pyrifera_M_contig104107:2..566Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig104107 3..566 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig104107.884.1prot_M-pyrifera_M_contig104107.884.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig104107 3..566 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig104107.884.1

>prot_M-pyrifera_M_contig104107.884.1 ID=prot_M-pyrifera_M_contig104107.884.1|Name=mRNA_M-pyrifera_M_contig104107.884.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=188bp
DQVGEELLSGIGEVVVVPDNLDADEQLTVLGELFEREAEAAALISELEAA
QLSADEAISDECVVSLATIYGGPSVAAYVEPAWPLPTAIVDAGCTLDPDT
SDADPDSNGRAWLSLEQLGLLDAPTLVLFQTSSVEGEDEAIEVVESQPLW
PTLPAVASDQIIVFDRLGYPGAAGQIRFLGELEAALG*
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mRNA from alignment at M-pyrifera_M_contig104107:3..566+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig104107.884.1 ID=mRNA_M-pyrifera_M_contig104107.884.1|Name=mRNA_M-pyrifera_M_contig104107.884.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=564bp|location=Sequence derived from alignment at M-pyrifera_M_contig104107:3..566+ (Macrocystis pyrifera P11B4 male)
GACCAGGTGGGCGAGGAACTCCTCAGTGGGATCGGTGAGGTCGTGGTCGT CCCCGACAATCTCGACGCCGACGAGCAGCTCACGGTGCTCGGCGAGCTGT TCGAGCGCGAAGCCGAGGCTGCCGCGCTGATCAGCGAACTCGAGGCCGCA CAGCTGTCGGCCGACGAAGCGATCAGCGACGAGTGCGTCGTCTCGTTGGC CACGATCTATGGCGGACCGAGTGTCGCCGCCTATGTCGAACCGGCCTGGC CCCTCCCGACCGCGATCGTCGACGCCGGCTGCACACTCGATCCCGACACT TCCGACGCCGACCCCGACTCCAACGGCCGGGCCTGGCTGTCGCTCGAGCA GCTCGGACTACTCGACGCCCCGACCCTCGTGCTGTTCCAGACCTCCTCGG TCGAGGGTGAAGACGAGGCGATCGAGGTCGTCGAGTCCCAGCCGCTGTGG CCGACACTGCCGGCTGTGGCCTCCGATCAGATCATCGTGTTCGATCGTCT CGGCTATCCCGGCGCTGCCGGCCAGATCCGTTTCCTCGGCGAGCTCGAAG CCGCCCTCGGCTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig104107:3..566+

>mRNA_M-pyrifera_M_contig104107.884.1 ID=mRNA_M-pyrifera_M_contig104107.884.1|Name=mRNA_M-pyrifera_M_contig104107.884.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1128bp|location=Sequence derived from alignment at M-pyrifera_M_contig104107:3..566+ (Macrocystis pyrifera P11B4 male)
GACCAGGTGGGCGAGGAACTCCTCAGTGGGATCGGTGAGGTCGTGGTCGT
CCCCGACAATCTCGACGCCGACGAGCAGCTCACGGTGCTCGGCGAGCTGT
TCGAGCGCGAAGCCGAGGCTGCCGCGCTGATCAGCGAACTCGAGGCCGCA
CAGCTGTCGGCCGACGAAGCGATCAGCGACGAGTGCGTCGTCTCGTTGGC
CACGATCTATGGCGGACCGAGTGTCGCCGCCTATGTCGAACCGGCCTGGC
CCCTCCCGACCGCGATCGTCGACGCCGGCTGCACACTCGATCCCGACACT
TCCGACGCCGACCCCGACTCCAACGGCCGGGCCTGGCTGTCGCTCGAGCA
GCTCGGACTACTCGACGCCCCGACCCTCGTGCTGTTCCAGACCTCCTCGG
TCGAGGGTGAAGACGAGGCGATCGAGGTCGTCGAGTCCCAGCCGCTGTGG
CCGACACTGCCGGCTGTGGCCTCCGATCAGATCATCGTGTTCGATCGTCT
CGGCTATCCCGGCGCTGCCGGCCAGATCCGTTTCCTCGGCGAGCTCGAAG
CCGCCCTCGGCTGAGACCAGGTGGGCGAGGAACTCCTCAGTGGGATCGGT
GAGGTCGTGGTCGTCCCCGACAATCTCGACGCCGACGAGCAGCTCACGGT
GCTCGGCGAGCTGTTCGAGCGCGAAGCCGAGGCTGCCGCGCTGATCAGCG
AACTCGAGGCCGCACAGCTGTCGGCCGACGAAGCGATCAGCGACGAGTGC
GTCGTCTCGTTGGCCACGATCTATGGCGGACCGAGTGTCGCCGCCTATGT
CGAACCGGCCTGGCCCCTCCCGACCGCGATCGTCGACGCCGGCTGCACAC
TCGATCCCGACACTTCCGACGCCGACCCCGACTCCAACGGCCGGGCCTGG
CTGTCGCTCGAGCAGCTCGGACTACTCGACGCCCCGACCCTCGTGCTGTT
CCAGACCTCCTCGGTCGAGGGTGAAGACGAGGCGATCGAGGTCGTCGAGT
CCCAGCCGCTGTGGCCGACACTGCCGGCTGTGGCCTCCGATCAGATCATC
GTGTTCGATCGTCTCGGCTATCCCGGCGCTGCCGGCCAGATCCGTTTCCT
CGGCGAGCTCGAAGCCGCCCTCGGCTGA
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