mRNA_M-pyrifera_M_contig103021.632.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig103021.632.1
Unique NamemRNA_M-pyrifera_M_contig103021.632.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig103021.632.1 vs. uniprot
Match: UPI001FE18357 (SPFH domain-containing protein n=1 Tax=Propionicicella superfundia TaxID=348582 RepID=UPI001FE18357)

HSP 1 Score: 173 bits (439), Expect = 1.300e-49
Identity = 97/170 (57.06%), Postives = 117/170 (68.82%), Query Frame = 1
Query:    1 MKVSLPDEIREAITRGTARGAEESQFLDQVGDLDRYAQAKQADAMMAAAQNPGGGGAMGDMLGMXXXXXXXSQMANAMNPQAQQAPAXXXXXXXXPPPLPGAV-SYHVALNGQQAGPFTMEQMQAGLAGGQMKPTDMVWTAGMAAWAPANTVPALAPLFAAPPPLP-QTP 504
            M VSLPDEI +A+TRG ARG EE+ +++Q+ D+ RY QAKQA+AM+AAA NPGGGG MGD++G         QMAN MN QAQ   A        PPPLPGA  ++HV LNG  AGP+   Q+QA +  GQ+ PT +VW+ GMA W PA  VPAL  LFAAPPPLP QTP
Sbjct:  213 MNVSLPDEITQAMTRGVARGVEEAGWVNQLDDMQRYQQAKQAEAMVAAANNPGGGGTMGDLMGAGLGMAMAGQMANQMNQQAQAGQASA------PPPLPGAAQTFHVELNGSAAGPYNPGQLQAAVQAGQVTPTTLVWSTGMAGWTPAGQVPALQQLFAAPPPLPPQTP 376          
BLAST of mRNA_M-pyrifera_M_contig103021.632.1 vs. uniprot
Match: A0A8F4G0R9_9ACTN (SPFH domain-containing protein n=1 Tax=Aquihabitans sp. G128 TaxID=2849779 RepID=A0A8F4G0R9_9ACTN)

HSP 1 Score: 169 bits (429), Expect = 5.890e-48
Identity = 101/165 (61.21%), Postives = 116/165 (70.30%), Query Frame = 1
Query:    1 MKVSLPDEIREAITRGTARGAEESQFLDQVGDLDRYAQAKQADAMMAAAQNPGGGGAMGDMLGMXXXXXXXSQMANAMNPQAQQAPAXXXXXXXXPPPLPGAVSYHVALNGQQAGPFTMEQMQAGLAGGQMKPTDMVWTAGMAAWAPANTVPALAPLFAAPPPLP 495
            M +SLPDEI  A+TRG ARG EE  FLD VGDL+RY QAKQADAM+AAA NPGGGGAMGDM   XXXXXXX           QQ  A        PPPLPGA ++HV + GQ  GPFT+ Q+Q+G+A GQ+KP  +VW+AGM  W  A+TVPALA LF APPPLP
Sbjct:  216 MNISLPDEITAAMTRGVARGVEEGGFLDNVGDLNRYQQAKQADAMLAAASNPGGGGAMGDMXXXXXXXXXXXXXXXXXXNVGQQQAAPAG-----PPPLPGAQTFHVDMGGQSQGPFTVAQIQSGVANGQVKPESLVWSAGMPGWVAASTVPALAALFQAPPPLP 375          
BLAST of mRNA_M-pyrifera_M_contig103021.632.1 vs. uniprot
Match: UPI001FF6E44A (SPFH domain-containing protein n=1 Tax=Williamsia sp. DF01-3 TaxID=2934157 RepID=UPI001FF6E44A)

HSP 1 Score: 166 bits (420), Expect = 1.210e-47
Identity = 94/167 (56.29%), Postives = 109/167 (65.27%), Query Frame = 1
Query:    1 MKVSLPDEIREAITRGTARGAEESQFLDQVGDLDRYAQAKQADAMMAAAQNPGGGGAMGDMLGMXXXXXXXSQMANAMNPQAQQAPAXXXXXXXXPPPLPGAVSYHVALNGQQAGPFTMEQMQAGLAGGQMKPTDMVWTAGMAAWAPANTVPALAPLFAAPPPLPQT 501
            M +SLPDEI  A+TRG ARG EE  FL+ VGDL RY Q + ADAM+AAAQNPGGG AMGDM+         SQMA     QAQQA          PPPLPGAV++H+   GQ AGP+T+ Q+Q  +A GQ     +VWT GM  WA A TVPALA LFA PPP+P T
Sbjct:  137 MNISLPDEITSAMTRGVARGVEEGGFLNNVGDLGRYQQGRAADAMLAAAQNPGGG-AMGDMMSAGMGVALGSQMAGQFGGQAQQA---NVAAPQGPPPLPGAVTFHIDNGGQPAGPYTVAQLQGAVASGQFTGATLVWTQGMPGWAQAATVPALAGLFATPPPIPTT 299          
BLAST of mRNA_M-pyrifera_M_contig103021.632.1 vs. uniprot
Match: A0A7W0QWQ0_9ACTN (SPFH domain-containing protein n=1 Tax=Acidimicrobiia bacterium TaxID=2080302 RepID=A0A7W0QWQ0_9ACTN)

HSP 1 Score: 164 bits (416), Expect = 4.990e-46
Identity = 99/166 (59.64%), Postives = 115/166 (69.28%), Query Frame = 1
Query:    1 MKVSLPDEIREAITRGTARGAEESQFLDQVGDLDRYAQAKQADAMMAAAQNPGGGGAMGDMLGMXXXXXXXSQMANA-MNPQAQQAPAXXXXXXXXPPPLPGAVSYHVALNGQQAGPFTMEQMQAGLAGGQMKPTDMVWTAGMAAWAPANTVPALAPLFAAPPPLP 495
            M +SLP+EI  A+TRG ARG EE  FLD VGDLDRY QA+QADAM+AAA NPGGGGAMGDM   XXXXXXX         P AQ APA        PPPLPGA ++HV + GQ  GPFT+ Q+Q+G+A GQ+  + +VW AGM  W  A T+PAL  LF APPPLP
Sbjct:  216 MNISLPEEITAAMTRGVARGVEEGGFLDNVGDLDRYQQARQADAMLAAANNPGGGGAMGDMXXXXXXXXXXXXXXXXXQQPGAQAAPAG-------PPPLPGAQTFHVDMGGQSQGPFTVAQIQSGVASGQVTGSSLVWAAGMPGWVAAETIPALQTLFQAPPPLP 374          
BLAST of mRNA_M-pyrifera_M_contig103021.632.1 vs. uniprot
Match: UPI0003770337 (SPFH domain-containing protein n=1 Tax=Nocardia sp. 348MFTsu5.1 TaxID=1172185 RepID=UPI0003770337)

HSP 1 Score: 163 bits (413), Expect = 8.580e-46
Identity = 95/169 (56.21%), Postives = 110/169 (65.09%), Query Frame = 1
Query:    1 MKVSLPDEIREAITRGTARGAEESQFLDQVGDLDRYAQAKQADAMMAAAQNPGGGGAMGDMLGMXXXXXXXSQMANAMNPQ-AQQAPAXXXXXXXXPPPLPGAVSYHVALNGQQAGPFTMEQMQAGLAGGQMKPTDMVWTAGMAAWAPANTVPALAPLFAAPPPLPQTP 504
            M +SLPDEI  A+TRG ARG EES FL+ VGDL RY Q K ADAM+AAAQNPGGG AMGDM+         SQMA     Q AQ APA        PPPLPGA ++HV   GQ AGPFT+ Q+Q  +A GQ   + +VW+ GM  W  A TVPAL+ LF+ PPPLP  P
Sbjct:  216 MNISLPDEITNAMTRGVARGVEESGFLNNVGDLGRYQQGKAADAMIAAAQNPGGG-AMGDMMSAGMGVALGSQMAGQFGGQGAQPAPATV------PPPLPGAATFHVDNGGQAAGPFTVAQLQPAVASGQFNASTLVWSQGMPGWVAAGTVPALSGLFSTPPPLPNNP 377          
BLAST of mRNA_M-pyrifera_M_contig103021.632.1 vs. uniprot
Match: A0A7X7D556_9ACTN (SPFH domain-containing protein n=1 Tax=Acidimicrobiales bacterium TaxID=2201156 RepID=A0A7X7D556_9ACTN)

HSP 1 Score: 159 bits (403), Expect = 4.190e-44
Identity = 100/165 (60.61%), Postives = 114/165 (69.09%), Query Frame = 1
Query:    1 MKVSLPDEIREAITRGTARGAEESQFLDQVGDLDRYAQAKQADAMMAAAQNPGGGGAMGDMLGMXXXXXXXSQMANAMNPQAQQAPAXXXXXXXXPPPLPGAVSYHVALNGQQAGPFTMEQMQAGLAGGQMKPTDMVWTAGMAAWAPANTVPALAPLFAAPPPLP 495
            M +SLP+EI  A+TRG ARG EE  FLD VGDL+RY QAKQADAM+AAAQNPGGGG MGDM   XXXXXXX                XXX       PLP A ++HV + GQ  GPFT+ Q+Q+G+A GQ+    +VWT GMAAWA ANTVPAL PLFAAPPPLP
Sbjct:  216 MNISLPEEITAAMTRGVARGVEEGGFLDNVGDLNRYQQAKQADAMLAAAQNPGGGGTMGDMXXXXXXXXXXXXXXXXXXXXXXXXXXXXX-------PLPAAQTFHVEMGGQSQGPFTVAQIQSGIANGQVTAASLVWTNGMAAWAAANTVPALQPLFAAPPPLP 373          
BLAST of mRNA_M-pyrifera_M_contig103021.632.1 vs. uniprot
Match: A0A261CVY7_9NOCA (Antifreeze protein n=9 Tax=Corynebacteriales TaxID=85007 RepID=A0A261CVY7_9NOCA)

HSP 1 Score: 158 bits (400), Expect = 7.460e-44
Identity = 89/168 (52.98%), Postives = 105/168 (62.50%), Query Frame = 1
Query:    1 MKVSLPDEIREAITRGTARGAEESQFLDQVGDLDRYAQAKQADAMMAAAQNPGGGGAMGDMLGMXXXXXXXSQMANAMNPQAQQAPAXXXXXXXXPPPLPGAVSYHVALNGQQAGPFTMEQMQAGLAGGQMKPTDMVWTAGMAAWAPANTVPALAPLFAAPPPLPQTP 504
            M +SLPDEI  A+TRG ARG EE  FL+ VGDL RY Q K ADAM+AAAQNPGGG AMGDM+         SQMA       Q  PA        PPPLPGA ++H+   G+ AGPFT+ Q+Q     GQ     +VW+ GM  W  A++VPALA LFA PPP+P TP
Sbjct:  216 MNISLPDEITSAMTRGVARGVEEGGFLNNVGDLGRYQQGKAADAMLAAAQNPGGG-AMGDMMSAGMGVALGSQMAGQFGQAGQGQPAPAAG----PPPLPGAATFHIDSGGRPAGPFTIAQLQGAATSGQFTGATLVWSQGMPGWVQASSVPALAGLFATPPPIPTTP 378          
BLAST of mRNA_M-pyrifera_M_contig103021.632.1 vs. uniprot
Match: A0A849CA46_9NOCA (SPFH domain-containing protein n=1 Tax=Nocardia uniformis TaxID=53432 RepID=A0A849CA46_9NOCA)

HSP 1 Score: 153 bits (387), Expect = 6.550e-42
Identity = 88/168 (52.38%), Postives = 110/168 (65.48%), Query Frame = 1
Query:    1 MKVSLPDEIREAITRGTARGAEESQFLDQVGDLDRYAQAKQADAMMAAAQNPGGGGAMGDMLGMXXXXXXXSQMANAMNPQAQQAPAXXXXXXXXPPPLPGAVSYHVALNGQQAGPFTMEQMQAGLAGGQMKPTDMVWTAGMAAWAPANTVPALAPLFAAPPPLPQTP 504
            + +SLP+EI +A+TRG ARG E+  +LD VGD+ R+ QAK A+AM+AAA+NP GGGAMG M+        X  MANAM  Q   AP         PPPLP   ++HV   GQ AGP+T+ Q+Q G++ GQ+    MVW  GMA W PA+TVPAL  LF APPPLP TP
Sbjct:  216 LTISLPEEITQAMTRGVARGVEQGGYLDNVGDMGRFQQAKAAEAMVAAAENPSGGGAMGAMMQAGMGVAMXGHMANAM--QGGIAPQAA------PPPLPTQQAFHVEQGGQPAGPYTIAQIQQGVSTGQVSANTMVWANGMANWVPASTVPALQALFTAPPPLPGTP 375          
BLAST of mRNA_M-pyrifera_M_contig103021.632.1 vs. uniprot
Match: UPI000E30E848 (SPFH domain-containing protein n=1 Tax=Euzebya tangerina TaxID=591198 RepID=UPI000E30E848)

HSP 1 Score: 153 bits (387), Expect = 8.060e-42
Identity = 86/165 (52.12%), Postives = 107/165 (64.85%), Query Frame = 1
Query:    1 MKVSLPDEIREAITRGTARGAEESQFLDQVGDLDRYAQAKQADAMMAAAQNPGGGGAMGDMLGMXXXXXXXSQMANAMNPQAQQAPAXXXXXXXXPPPLPGAVSYHVALNGQQAGPFTMEQMQAGLAGGQMKPTDMVWTAGMAAWAPANTVPALAPLFAAPPPLP 495
            M++SLP+EI +A+TRG ARG EE  +   VGDLDR+ Q + +DAM+AAA NPGGG A GD +G+        QMANA   Q   A A        PPPLPGA S+HVA+NGQQ GP+ + Q+Q  +  GQ+    +VW  GM+ W PA  VPALA LF APPPLP
Sbjct:  216 MRISLPEEITQAMTRGVARGVEEGGYARNVGDLDRFQQVRASDAMIAAASNPGGGAA-GDAMGIGLGMAMAGQMANAFGQQTGGAGA--------PPPLPGAQSFHVAVNGQQLGPYGIPQLQQAVGQGQVSADTLVWANGMSGWTPAGQVPALAGLFNAPPPLP 371          
BLAST of mRNA_M-pyrifera_M_contig103021.632.1 vs. uniprot
Match: UPI0003482206 (SPFH domain-containing protein n=1 Tax=Ilumatobacter nonamiensis TaxID=467093 RepID=UPI0003482206)

HSP 1 Score: 145 bits (367), Expect = 9.170e-39
Identity = 88/170 (51.76%), Postives = 107/170 (62.94%), Query Frame = 1
Query:    1 MKVSLPDEIREAITRGTARGAEESQFLDQVGDLDRYAQAKQADAMMAAAQNPGGGGAMGDMLGMXXXXXXXSQMANAMNPQ--AQQAPAXXXXXXXXP----PPLPGAVSYHVALNGQQAGPFTMEQMQAGLAGGQMKPTDMVWTAGMAAWAPANTVPALAPLFAAPPPL 492
            M +SLPDE+ +A+TRG A+G E S F++ V DLD+Y QA+ ADAM+AAA+N GG   MGDM+ M        QMAN M     AQQAPA        P    PPLPG   +HV   GQ  GP+ M QMQ G+ GGQ+    +VW  GMAAWAPA +VP L  LFAAPPP+
Sbjct:  216 MNISLPDEVTQAMTRGVAKGVEASGFVENV-DLDKYQQAQAADAMLAAAENEGGS-VMGDMMQMGMGVAMAGQMANQMPGAGGAQQAPAAQPAVAANPGGAPPPLPGQKLFHVDHGGQAGGPYNMAQMQQGIQGGQVTGQTLVWAQGMAAWAPAQSVPELQALFAAPPPV 383          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig103021.632.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UPI001FE183571.300e-4957.06SPFH domain-containing protein n=1 Tax=Propionicic... [more]
A0A8F4G0R9_9ACTN5.890e-4861.21SPFH domain-containing protein n=1 Tax=Aquihabitan... [more]
UPI001FF6E44A1.210e-4756.29SPFH domain-containing protein n=1 Tax=Williamsia ... [more]
A0A7W0QWQ0_9ACTN4.990e-4659.64SPFH domain-containing protein n=1 Tax=Acidimicrob... [more]
UPI00037703378.580e-4656.21SPFH domain-containing protein n=1 Tax=Nocardia sp... [more]
A0A7X7D556_9ACTN4.190e-4460.61SPFH domain-containing protein n=1 Tax=Acidimicrob... [more]
A0A261CVY7_9NOCA7.460e-4452.98Antifreeze protein n=9 Tax=Corynebacteriales TaxID... [more]
A0A849CA46_9NOCA6.550e-4252.38SPFH domain-containing protein n=1 Tax=Nocardia un... [more]
UPI000E30E8488.060e-4252.12SPFH domain-containing protein n=1 Tax=Euzebya tan... [more]
UPI00034822069.170e-3951.76SPFH domain-containing protein n=1 Tax=Ilumatobact... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig103021contigM-pyrifera_M_contig103021:5..547 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score171.0
Seed ortholog evalue5e-40
Seed eggNOG ortholog258052.JNYV01000001_gene475
Preferred nameydjI
Hectar predicted targeting categoryother localisation
EggNOG free text desc.SPFH domain-Band 7 family
EggNOG OGs2HUEV@201174,COG4260@1,COG4260@2
COG Functional cat.L
Best tax levelActinobacteria
Best eggNOG OGNA|NA|NA
Exons1
Model size543
Cds size543
Stop1
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815534.549703-CDS-M-pyrifera_M_contig103021:4..5471622815534.549703-CDS-M-pyrifera_M_contig103021:4..547Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig103021 5..547 +
1692277453.1516483-CDS-M-pyrifera_M_contig103021:4..5471692277453.1516483-CDS-M-pyrifera_M_contig103021:4..547Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig103021 5..547 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig103021.632.1prot_M-pyrifera_M_contig103021.632.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig103021 5..547 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig103021.632.1

>prot_M-pyrifera_M_contig103021.632.1 ID=prot_M-pyrifera_M_contig103021.632.1|Name=mRNA_M-pyrifera_M_contig103021.632.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=181bp
MKVSLPDEIREAITRGTARGAEESQFLDQVGDLDRYAQAKQADAMMAAAQ
NPGGGGAMGDMLGMGMGMAMASQMANAMNPQAQQAPAAAAPAAMAPPPLP
GAVSYHVALNGQQAGPFTMEQMQAGLAGGQMKPTDMVWTAGMAAWAPANT
VPALAPLFAAPPPLPQTPPTPPPAAPPAGM*
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mRNA from alignment at M-pyrifera_M_contig103021:5..547+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig103021.632.1 ID=mRNA_M-pyrifera_M_contig103021.632.1|Name=mRNA_M-pyrifera_M_contig103021.632.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=543bp|location=Sequence derived from alignment at M-pyrifera_M_contig103021:5..547+ (Macrocystis pyrifera P11B4 male)
ATGAAGGTGTCGCTCCCCGACGAGATCCGCGAGGCGATCACCCGCGGTAC TGCCCGAGGCGCCGAAGAAAGCCAGTTCCTCGACCAGGTGGGCGATCTCG ATCGTTACGCCCAGGCCAAGCAGGCCGACGCCATGATGGCTGCCGCCCAG AACCCGGGTGGCGGCGGTGCGATGGGCGACATGCTCGGCATGGGTATGGG CATGGCCATGGCCAGCCAGATGGCCAACGCCATGAACCCGCAGGCCCAGC AGGCGCCGGCTGCCGCAGCGCCGGCAGCGATGGCTCCGCCTCCTCTTCCC GGAGCGGTCTCCTATCACGTCGCACTCAACGGCCAGCAGGCCGGGCCGTT CACGATGGAACAGATGCAAGCCGGCCTCGCCGGTGGTCAGATGAAGCCCA CCGACATGGTGTGGACCGCAGGCATGGCCGCCTGGGCCCCCGCCAACACC GTTCCGGCCCTGGCGCCGCTGTTCGCCGCCCCGCCGCCGCTCCCCCAGAC GCCTCCGACCCCGCCGCCCGCAGCACCACCGGCCGGAATGTAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig103021:5..547+

>mRNA_M-pyrifera_M_contig103021.632.1 ID=mRNA_M-pyrifera_M_contig103021.632.1|Name=mRNA_M-pyrifera_M_contig103021.632.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1086bp|location=Sequence derived from alignment at M-pyrifera_M_contig103021:5..547+ (Macrocystis pyrifera P11B4 male)
ATGAAGGTGTCGCTCCCCGACGAGATCCGCGAGGCGATCACCCGCGGTAC
TGCCCGAGGCGCCGAAGAAAGCCAGTTCCTCGACCAGGTGGGCGATCTCG
ATCGTTACGCCCAGGCCAAGCAGGCCGACGCCATGATGGCTGCCGCCCAG
AACCCGGGTGGCGGCGGTGCGATGGGCGACATGCTCGGCATGGGTATGGG
CATGGCCATGGCCAGCCAGATGGCCAACGCCATGAACCCGCAGGCCCAGC
AGGCGCCGGCTGCCGCAGCGCCGGCAGCGATGGCTCCGCCTCCTCTTCCC
GGAGCGGTCTCCTATCACGTCGCACTCAACGGCCAGCAGGCCGGGCCGTT
CACGATGGAACAGATGCAAGCCGGCCTCGCCGGTGGTCAGATGAAGCCCA
CCGACATGGTGTGGACCGCAGGCATGGCCGCCTGGGCCCCCGCCAACACC
GTTCCGGCCCTGGCGCCGCTGTTCGCCGCCCCGCCGCCGCTCCCCCAGAC
GCCTCCGACCCCGCCGCCCGCAGCACCACCGGCCGGAATGTAGATGAAGG
TGTCGCTCCCCGACGAGATCCGCGAGGCGATCACCCGCGGTACTGCCCGA
GGCGCCGAAGAAAGCCAGTTCCTCGACCAGGTGGGCGATCTCGATCGTTA
CGCCCAGGCCAAGCAGGCCGACGCCATGATGGCTGCCGCCCAGAACCCGG
GTGGCGGCGGTGCGATGGGCGACATGCTCGGCATGGGTATGGGCATGGCC
ATGGCCAGCCAGATGGCCAACGCCATGAACCCGCAGGCCCAGCAGGCGCC
GGCTGCCGCAGCGCCGGCAGCGATGGCTCCGCCTCCTCTTCCCGGAGCGG
TCTCCTATCACGTCGCACTCAACGGCCAGCAGGCCGGGCCGTTCACGATG
GAACAGATGCAAGCCGGCCTCGCCGGTGGTCAGATGAAGCCCACCGACAT
GGTGTGGACCGCAGGCATGGCCGCCTGGGCCCCCGCCAACACCGTTCCGG
CCCTGGCGCCGCTGTTCGCCGCCCCGCCGCCGCTCCCCCAGACGCCTCCG
ACCCCGCCGCCCGCAGCACCACCGGCCGGAATGTAG
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