mRNA_M-pyrifera_M_contig102423.513.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig102423.513.1
Unique NamemRNA_M-pyrifera_M_contig102423.513.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig102423.513.1 vs. uniprot
Match: A0A1X2HVW3_SYNRA (Uncharacterized protein n=1 Tax=Syncephalastrum racemosum TaxID=13706 RepID=A0A1X2HVW3_SYNRA)

HSP 1 Score: 102 bits (253), Expect = 3.930e-22
Identity = 60/165 (36.36%), Postives = 92/165 (55.76%), Query Frame = 1
Query:    7 VRFNDLSWREHLPSLKPLGLNFLLNSHC------RFGEYNSRRMPTSVDSLYSVKVHDSLCLCALGQRIGFSKGALDNFEPVKEIYRFSHCAAYRDLLAELDEKRGPSDQR-KSKVAHALDTAVQDTVGGSIQLLSKGNSTMILESCGNVWHGNSIKPLTDSAVK 480
            V+F D  W +HLPSLKP+GLNF+LN++C      +  EY+ RR    V    +       CLC LG+ IGF + AL +F    EIY F   A Y ++L   D  R   +Q  + +V +AL T  ++   GS QLL+ G+  ++L+ C + W G+ ++ +++S  K
Sbjct:  752 VKFEDRDWEQHLPSLKPVGLNFMLNTNCGVVQGRKRSEYHRRRSKLHVYGRTTPA--RQTCLCRLGKTIGFREEALKSFALRAEIYTF---APYHEIL---DAPRYQYNQYFQFEVPNALSTVFEEKTSGSYQLLTDGHPALVLDKCSDYWDGSGLQTMSNSMEK 908          
BLAST of mRNA_M-pyrifera_M_contig102423.513.1 vs. uniprot
Match: A0A8H7Q8A0_9FUNG (Cation_ATPase_C domain-containing protein n=1 Tax=Umbelopsis vinacea TaxID=44442 RepID=A0A8H7Q8A0_9FUNG)

HSP 1 Score: 99.8 bits (247), Expect = 2.530e-21
Identity = 61/164 (37.20%), Postives = 90/164 (54.88%), Query Frame = 1
Query:    7 VRFNDLSWREHLPSLKPLGLNFLLNSHC------RFGEYNSRRMPTSVDSLYSVKVHDSLCLCALGQRIGFSKGALDNFEPVKEIYRFSHCAAYRDLLAELDEKRGPSDQRKSKVAHALDTAVQDTVGGSIQLLSKGNSTMILESCGNVWHGNSIKPLTDSAVK 480
            ++F D  W ++L  LKP+GLNFLLN++C      R  E++ +R  + + S          CLC LG+ IGF+  AL  F+  KEIY F   A Y ++L   +  R  S Q   +V   L T  ++T  GS QLLS G+  +IL+ C + W G S++ +TD+  K
Sbjct:  692 IKFEDQDWDQYLAGLKPVGLNFLLNTNCGVLQGRRRAEHHRKR--SGLHSYGKTLPARQSCLCRLGKTIGFTDDALSTFQLRKEIYTF---APYHEVL---NAHRNFSQQ--FEVPSMLSTIYEETRSGSYQLLSDGHLDLILDKCSDYWDGQSLRRMTDAMEK 845          
BLAST of mRNA_M-pyrifera_M_contig102423.513.1 vs. uniprot
Match: A0A162UNV8_PHYB8 (Cation_ATPase_C domain-containing protein n=1 Tax=Phycomyces blakesleeanus (strain ATCC 8743b / DSM 1359 / FGSC 10004 / NBRC 33097 / NRRL 1555) TaxID=763407 RepID=A0A162UNV8_PHYB8)

HSP 1 Score: 99.8 bits (247), Expect = 2.550e-21
Identity = 60/166 (36.14%), Postives = 91/166 (54.82%), Query Frame = 1
Query:    1 FVVRFNDLSWREHLPSLKPLGLNFLLNSHC------RFGEYNSRRMPTSVDSLYSVKVHDSLCLCALGQRIGFSKGALDNFEPVKEIYRFSHCAAYRDLLAELDEKRGPSDQRKSKVAHALDTAVQDTVGGSIQLLSKGNSTMILESCGNVWHGNSIKPLTDSAVK 480
            F V+F D  W ++LP LKPLGLNF+LN++C      +  +Y+ RR    V    S       CLC LG+ IGF + AL +F    EIY F   A Y D+L+    K   S     +V +AL T  ++    S QLL+ G+ T++LE C + W G++++ ++++  K
Sbjct:  811 FGVKFEDQDWEKNLPCLKPLGLNFMLNTNCGIIQGRKRSDYHRRRSKLHVYGKTSPARQS--CLCRLGKCIGFREEALQSFVSRAEIYTF---APYHDILSTPRYKY--SQYFSFEVPNALSTVFEEKTTESYQLLTDGHPTLVLEKCSDYWDGSALQTMSETMEK 969          
BLAST of mRNA_M-pyrifera_M_contig102423.513.1 vs. uniprot
Match: A0A168Q8Y5_MUCCL (Cation_ATPase_C domain-containing protein n=2 Tax=Mucor circinelloides f. lusitanicus TaxID=29924 RepID=A0A168Q8Y5_MUCCL)

HSP 1 Score: 99.0 bits (245), Expect = 4.730e-21
Identity = 58/164 (35.37%), Postives = 92/164 (56.10%), Query Frame = 1
Query:    7 VRFNDLSWREHLPSLKPLGLNFLLNSHC------RFGEYNSRRMPTSVDSLYSVKVHDSLCLCALGQRIGFSKGALDNFEPVKEIYRFSHCAAYRDLLAELDEKRGPSDQRKSKVAHALDTAVQDTVGGSIQLLSKGNSTMILESCGNVWHGNSIKPLTDSAVK 480
            ++F D  W ++LP LKPLGLNF+LN++C      +  EY+ RR    V    +     S CLC LG+ IGF + AL +F    EIY F   A Y ++L     K   S   + +V +AL T  ++   GS QLL+ G+ +++++ C + W GNS++ ++++  K
Sbjct:  841 LKFEDQDWEQYLPCLKPLGLNFMLNTNCGVIQSRKRSEYHRRRSKLHVYGK-TAPARQS-CLCRLGKCIGFREEALQSFALRAEIYTF---APYHEILNTPRYKY--SKYYQFEVPNALSTVFEERTSGSYQLLTDGHPSLVMDKCSDYWDGNSLQTMSETMEK 997          
BLAST of mRNA_M-pyrifera_M_contig102423.513.1 vs. uniprot
Match: A0A8H7PQN4_MORIS (Cation_ATPase_C domain-containing protein n=1 Tax=Mortierella isabellina TaxID=91625 RepID=A0A8H7PQN4_MORIS)

HSP 1 Score: 98.6 bits (244), Expect = 6.390e-21
Identity = 59/164 (35.98%), Postives = 91/164 (55.49%), Query Frame = 1
Query:    7 VRFNDLSWREHLPSLKPLGLNFLLNSHC------RFGEYNSRRMPTSVDSLYSVKVHDSLCLCALGQRIGFSKGALDNFEPVKEIYRFSHCAAYRDLLAELDEKRGPSDQRKSKVAHALDTAVQDTVGGSIQLLSKGNSTMILESCGNVWHGNSIKPLTDSAVK 480
            ++F D  W ++L SLKP+GLNFLLN++C      R  E++ +R  + + S          CLC LG+ IGF+  AL  F+  KEIY F   A Y ++L    ++  P   ++ +V   L T  ++T  GS QLLS G+  +IL+ C + W G  ++ +TD+  K
Sbjct:  695 IKFEDQDWDQYLASLKPVGLNFLLNTNCGVLQGRRRAEHHRKR--SGLHSYGKTLPARQSCLCRLGKSIGFTDDALATFQLRKEIYTF---APYHEVLN--SQRNFP---QQFEVPSMLSTIYEETRSGSYQLLSDGHLDLILDKCSDYWDGQELRRMTDAMEK 848          
BLAST of mRNA_M-pyrifera_M_contig102423.513.1 vs. uniprot
Match: A0A0B7N9H9_9FUNG (Cation_ATPase_C domain-containing protein n=1 Tax=Parasitella parasitica TaxID=35722 RepID=A0A0B7N9H9_9FUNG)

HSP 1 Score: 98.2 bits (243), Expect = 8.770e-21
Identity = 58/164 (35.37%), Postives = 91/164 (55.49%), Query Frame = 1
Query:    7 VRFNDLSWREHLPSLKPLGLNFLLNSHC------RFGEYNSRRMPTSVDSLYSVKVHDSLCLCALGQRIGFSKGALDNFEPVKEIYRFSHCAAYRDLLAELDEKRGPSDQRKSKVAHALDTAVQDTVGGSIQLLSKGNSTMILESCGNVWHGNSIKPLTDSAVK 480
            ++F D  W  +LP LKPLGLNF+LN++C      +  EY+ RR    V    +     S CLC LG+ IGF + AL +F    EIY F   A Y ++L     K   S   + +V +AL T  ++   GS QLL+ G+ +++++ C + W GNS++ ++++  K
Sbjct:  845 LKFEDQGWERYLPCLKPLGLNFMLNTNCGVIQSRKRSEYHRRRSKLHVYGK-TAPARQS-CLCKLGKCIGFREEALQSFALRAEIYTF---APYHEILNTPRYKY--SKYYQFEVPNALSTVFEERTSGSYQLLTDGHPSLVIDKCSDYWDGNSLQTMSETMEK 1001          
BLAST of mRNA_M-pyrifera_M_contig102423.513.1 vs. uniprot
Match: S2JRQ1_MUCC1 (Cation_ATPase_C domain-containing protein n=2 Tax=Mucor TaxID=4830 RepID=S2JRQ1_MUCC1)

HSP 1 Score: 97.8 bits (242), Expect = 1.200e-20
Identity = 57/164 (34.76%), Postives = 92/164 (56.10%), Query Frame = 1
Query:    7 VRFNDLSWREHLPSLKPLGLNFLLNSHC------RFGEYNSRRMPTSVDSLYSVKVHDSLCLCALGQRIGFSKGALDNFEPVKEIYRFSHCAAYRDLLAELDEKRGPSDQRKSKVAHALDTAVQDTVGGSIQLLSKGNSTMILESCGNVWHGNSIKPLTDSAVK 480
            ++F D  W ++LP LKPLGLNF+LN++C      +  EY+ RR    V    +     S CLC LG+ IGF + AL +F    EIY F   A Y ++L     K   S   + +V +AL T  ++   G+ QLL+ G+ +++++ C + W GNS++ ++++  K
Sbjct:  843 LKFEDQDWEQYLPCLKPLGLNFMLNTNCGVIQSRKRSEYHRRRSKLHVYGK-TAPARQS-CLCRLGKCIGFREEALQSFALRAEIYTF---APYHEILNTPRYKY--SKYYQFEVPNALSTVFEERTSGNYQLLTDGHPSLVMDKCSDYWDGNSLQTMSETMEK 999          
BLAST of mRNA_M-pyrifera_M_contig102423.513.1 vs. uniprot
Match: A0A8H7ENX2_9FUNG (Uncharacterized protein n=1 Tax=Apophysomyces ossiformis TaxID=679940 RepID=A0A8H7ENX2_9FUNG)

HSP 1 Score: 96.7 bits (239), Expect = 2.950e-20
Identity = 58/165 (35.15%), Postives = 92/165 (55.76%), Query Frame = 1
Query:    7 VRFNDLSWREHLPSLKPLGLNFLLNSHC------RFGEYNSRRMPTSVDSLYSVKVHDSLCLCALGQRIGFSKGALDNFEPVKEIYRFSHCAAYRDLLAELDEKRGPSDQRKS-KVAHALDTAVQDTVGGSIQLLSKGNSTMILESCGNVWHGNSIKPLTDSAVK 480
            +RF +  W ++LP LKPLGLNF+LN++C      +  EY+ RR    V    S       CLC +G+ IGF + AL +F    EIY F   A Y ++L      R   +Q  S +V +AL T  ++   GS QLL+ G+ +++L+ C + W G+S++ ++++  K
Sbjct:  656 IRFEEQDWEQYLPCLKPLGLNFMLNTNCGVIQGRKRSEYHRRRSKLHVYGKTSCARQS--CLCKMGKCIGFREEALQSFALRAEIYTF---APYHEILK---TPRYQYNQYFSFEVPNALSTVFEERDSGSYQLLTDGHPSLVLDKCSDYWDGSSLQTMSETMEK 812          
BLAST of mRNA_M-pyrifera_M_contig102423.513.1 vs. uniprot
Match: A0A1Y1XYG6_9FUNG (Calcium ATPase n=1 Tax=Basidiobolus meristosporus CBS 931.73 TaxID=1314790 RepID=A0A1Y1XYG6_9FUNG)

HSP 1 Score: 96.7 bits (239), Expect = 2.970e-20
Identity = 63/163 (38.65%), Postives = 82/163 (50.31%), Query Frame = 1
Query:    7 VRFNDLSWREHLPSLKPLGLNFLLNSHCRFGEYNSRRMPTSVDSLYSVKVHDSL------CLCALGQRIGFSKGALDNFEPVKEIYRFSHCAAYRDLLAELDEKRGPSDQRKSKVAHALDTAVQDTVGGSIQLLSKGNSTMILESCGNVWHGNSIKPLTDSAV 477
            ++F D  W+++LPSLKPLGLN LLN+ C  G    RR         S+  H  L      CLC LG+ IGFS GAL +F   K+I  FS    YR  L  L   R      + +V   L T  ++   GS QLL  GN  +IL+ C + W+G  +  L D  V
Sbjct:  518 MQFEDRDWQKYLPSLKPLGLNLLLNTDC--GVRQGRRRADHHRKFSSIHKHARLLPSRQSCLCRLGKEIGFSDGALASFSKKKDIGTFS---PYRPSL--LHSLR----HHQYEVPSVLSTIYEEMNSGSYQLLCDGNIELILDFCSDYWNGEQLNVLDDGMV 669          
BLAST of mRNA_M-pyrifera_M_contig102423.513.1 vs. uniprot
Match: A0A367KK53_RHIST (Tr-type G domain-containing protein (Fragment) n=2 Tax=Rhizopus stolonifer TaxID=4846 RepID=A0A367KK53_RHIST)

HSP 1 Score: 96.7 bits (239), Expect = 3.010e-20
Identity = 58/164 (35.37%), Postives = 88/164 (53.66%), Query Frame = 1
Query:    7 VRFNDLSWREHLPSLKPLGLNFLLNSHC------RFGEYNSRRMPTSVDSLYSVKVHDSLCLCALGQRIGFSKGALDNFEPVKEIYRFSHCAAYRDLLAELDEKRGPSDQRKSKVAHALDTAVQDTVGGSIQLLSKGNSTMILESCGNVWHGNSIKPLTDSAVK 480
            ++F D  W ++LP LKPLGLNF+LN++C      +  EY+ RR    V    S       CLC LG+ IGF + AL +F    EIY F   A Y +LL     K   S     +V +AL T  ++   GS QLL+ G+ +++++ C + W G S++ ++ +  K
Sbjct:  643 LKFEDQDWDQYLPCLKPLGLNFMLNTNCGVIQDRKPSEYHRRRSKLHVYGKTSPARQS--CLCRLGKCIGFREEALQSFALRSEIYTF---APYHELLNTARYKY--SKYYPFEVPNALSTVFEERASGSYQLLTDGHPSLVMDKCSDYWDGTSLQTMSGTMEK 799          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig102423.513.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A1X2HVW3_SYNRA3.930e-2236.36Uncharacterized protein n=1 Tax=Syncephalastrum ra... [more]
A0A8H7Q8A0_9FUNG2.530e-2137.20Cation_ATPase_C domain-containing protein n=1 Tax=... [more]
A0A162UNV8_PHYB82.550e-2136.14Cation_ATPase_C domain-containing protein n=1 Tax=... [more]
A0A168Q8Y5_MUCCL4.730e-2135.37Cation_ATPase_C domain-containing protein n=2 Tax=... [more]
A0A8H7PQN4_MORIS6.390e-2135.98Cation_ATPase_C domain-containing protein n=1 Tax=... [more]
A0A0B7N9H9_9FUNG8.770e-2135.37Cation_ATPase_C domain-containing protein n=1 Tax=... [more]
S2JRQ1_MUCC11.200e-2034.76Cation_ATPase_C domain-containing protein n=2 Tax=... [more]
A0A8H7ENX2_9FUNG2.950e-2035.15Uncharacterized protein n=1 Tax=Apophysomyces ossi... [more]
A0A1Y1XYG6_9FUNG2.970e-2038.65Calcium ATPase n=1 Tax=Basidiobolus meristosporus ... [more]
A0A367KK53_RHIST3.010e-2035.37Tr-type G domain-containing protein (Fragment) n=2... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig102423contigM-pyrifera_M_contig102423:3..560 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeFungi
Seed ortholog score99.4
Seed ortholog evalue1.6e-18
Seed eggNOG ortholog36080.S2JRQ1
Hectar predicted targeting categoryother localisation
EggNOG OGs1GSDU@112252,38C04@33154,3P1N9@4751,KOG4383@1,KOG4383@2759
Best tax levelFungi incertae sedis
Best eggNOG OGNA|NA|NA
Exons2
Model size480
Cds size480
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815526.6041925-CDS-M-pyrifera_M_contig102423:2..1351622815526.6041925-CDS-M-pyrifera_M_contig102423:2..135Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig102423 3..135 -
1692277449.574608-CDS-M-pyrifera_M_contig102423:2..1351692277449.574608-CDS-M-pyrifera_M_contig102423:2..135Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig102423 3..135 -
1622815526.615059-CDS-M-pyrifera_M_contig102423:213..5601622815526.615059-CDS-M-pyrifera_M_contig102423:213..560Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig102423 214..560 -
1692277449.5886564-CDS-M-pyrifera_M_contig102423:213..5601692277449.5886564-CDS-M-pyrifera_M_contig102423:213..560Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig102423 214..560 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig102423.513.1prot_M-pyrifera_M_contig102423.513.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig102423 3..560 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig102423.513.1

>prot_M-pyrifera_M_contig102423.513.1 ID=prot_M-pyrifera_M_contig102423.513.1|Name=mRNA_M-pyrifera_M_contig102423.513.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=160bp
FVVRFNDLSWREHLPSLKPLGLNFLLNSHCRFGEYNSRRMPTSVDSLYSV
KVHDSLCLCALGQRIGFSKGALDNFEPVKEIYRFSHCAAYRDLLAELDEK
RGPSDQRKSKVAHALDTAVQDTVGGSIQLLSKGNSTMILESCGNVWHGNS
IKPLTDSAVK
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mRNA from alignment at M-pyrifera_M_contig102423:3..560-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig102423.513.1 ID=mRNA_M-pyrifera_M_contig102423.513.1|Name=mRNA_M-pyrifera_M_contig102423.513.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=558bp|location=Sequence derived from alignment at M-pyrifera_M_contig102423:3..560- (Macrocystis pyrifera P11B4 male)
TTCGTGGTGCGCTTCAACGACCTCTCCTGGAGGGAGCACCTCCCCTCCCT CAAGCCGCTGGGCCTGAACTTCCTGCTCAACTCCCACTGCAGGTTTGGCG AGTACAACTCGCGCCGCATGCCCACCTCTGTGGATAGCCTCTACTCCGTC AAGGTCCACGACTCTCTCTGTCTCTGTGCGCTGGGCCAGAGGATTGGCTT CAGCAAGGGAGCTCTGGACAACTTCGAGCCAGTGAAGGAGATCTACCGCT TCTCGCACTGCGCTGCCTACCGCGACCTCCTGGCAGAGCTGGACGAGAAG AGGGGGCCCTCCGACCAGAGGAAGAGCAAGGTCGCGCACGCCCTCGAGTA CGGCTACGACGTCTTCCCCGAGGACCCAGTGGAGGAGGAGGTCAAGAGCC TGGAGCGCCTCTTCTACTCGGTCAGCACCGCAGTGCAGGACACGGTGGGG GGCTCGATCCAGCTGCTGAGCAAGGGCAACTCCACGATGATTCTGGAGAG CTGCGGGAACGTCTGGCACGGCAACTCCATCAAGCCTCTCACCGACAGCG CGGTGAAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig102423:3..560-

>mRNA_M-pyrifera_M_contig102423.513.1 ID=mRNA_M-pyrifera_M_contig102423.513.1|Name=mRNA_M-pyrifera_M_contig102423.513.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=960bp|location=Sequence derived from alignment at M-pyrifera_M_contig102423:3..560- (Macrocystis pyrifera P11B4 male)
TTCGTGGTGCGCTTCAACGACCTCTCCTGGAGGGAGCACCTCCCCTCCCT
CAAGCCGCTGGGCCTGAACTTCCTGCTCAACTCCCACTGCAGGTTTGGCG
AGTACAACTCGCGCCGCATGCCCACCTCTGTGGATAGCCTCTACTCCGTC
AAGGTCCACGACTCTCTCTGTCTCTGTGCGCTGGGCCAGAGGATTGGCTT
CAGCAAGGGAGCTCTGGACAACTTCGAGCCAGTGAAGGAGATCTACCGCT
TCTCGCACTGCGCTGCCTACCGCGACCTCCTGGCAGAGCTGGACGAGAAG
AGGGGGCCCTCCGACCAGAGGAAGAGCAAGGTCGCGCACGCCCTCGATTC
GTGGTGCGCTTCAACGACCTCTCCTGGAGGGAGCACCTCCCCTCCCTCAA
GCCGCTGGGCCTGAACTTCCTGCTCAACTCCCACTGCAGGTTTGGCGAGT
ACAACTCGCGCCGCATGCCCACCTCTGTGGATAGCCTCTACTCCGTCAAG
GTCCACGACTCTCTCTGTCTCTGTGCGCTGGGCCAGAGGATTGGCTTCAG
CAAGGGAGCTCTGGACAACTTCGAGCCAGTGAAGGAGATCTACCGCTTCT
CGCACTGCGCTGCCTACCGCGACCTCCTGGCAGAGCTGGACGAGAAGAGG
GGGCCCTCCGACCAGAGGAAGAGCAAGGTCGCGCACGCCCTCGACACCGC
AGTGCAGGACACGGTGGGGGGCTCGATCCAGCTGCTGAGCAAGGGCAACT
CCACGATGATTCTGGAGAGCTGCGGGAACGTCTGGCACGGCAACTCCATC
AAGCCTCTCACCGACAGCGCGGTGAAGCACCGCAGTGCAGGACACGGTGG
GGGGCTCGATCCAGCTGCTGAGCAAGGGCAACTCCACGATGATTCTGGAG
AGCTGCGGGAACGTCTGGCACGGCAACTCCATCAAGCCTCTCACCGACAG
CGCGGTGAAG
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