mRNA_M-pyrifera_M_contig102209.472.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig102209.472.1
Unique NamemRNA_M-pyrifera_M_contig102209.472.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig102209.472.1 vs. uniprot
Match: A0A2P6NK30_9EUKA (G protein b-subunit n=1 Tax=Planoprotostelium fungivorum TaxID=1890364 RepID=A0A2P6NK30_9EUKA)

HSP 1 Score: 189 bits (479), Expect = 7.520e-57
Identity = 85/128 (66.41%), Postives = 100/128 (78.12%), Query Frame = 1 
Query:    1 WDLRSGRAQQTFLGHTEDINCCQYFPNGMAFGTGSDDHSCRLFDIRADRELIKYQSQGVPKGVTAIAFSISGRYLFASYDDTTCHVWDSLKGENVGDLKGHSQRVSCLGVSADGYALGTGSWDGTLKV 384
            WD+R+ +  QTF GHT DIN   +FPNG AF TGSDD+SCRLFDIRADREL+KY    +  G+T++AFS SGRYLFA YDD  C+VWD+LKGE V  L+GH  RVSCLGVS+DG AL TGSWDG L+V
Sbjct:  221 WDIRTHKCVQTFSGHTSDINSVNFFPNGNAFATGSDDNSCRLFDIRADRELMKYSQDNIACGITSVAFSSSGRYLFAGYDDFHCNVWDTLKGERVFTLQGHDNRVSCLGVSSDGMALCTGSWDGLLRV 348
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BLAST of mRNA_M-pyrifera_M_contig102209.472.1 vs. uniprot
Match: A0A183VVZ2_TRIRE (WD_REPEATS_REGION domain-containing protein n=2 Tax=Digenea TaxID=6179 RepID=A0A183VVZ2_TRIRE)

HSP 1 Score: 167 bits (424), Expect = 2.610e-51
Identity = 74/128 (57.81%), Postives = 91/128 (71.09%), Query Frame = 1 
Query:    1 WDLRSGRAQQTFLGHTEDINCCQYFPNGMAFGTGSDDHSCRLFDIRADRELIKYQSQGVPKGVTAIAFSISGRYLFASYDDTTCHVWDSLKGENVGDLKGHSQRVSCLGVSADGYALGTGSWDGTLKV 384
            WDLR G+ +QTF GH  DIN   YFPNGMAF TGSDD +CRLFDIR+D+E+  Y    +  G+T++AFS SGR L   YDD  C++WD+LK E  G L GH  RVSCLGV+ DG A+ TGSWD  L++
Sbjct:    3 WDLRDGQCKQTFSGHESDINAINYFPNGMAFATGSDDATCRLFDIRSDQEVGMYSHDNIICGITSVAFSRSGRLLLGGYDDFNCNIWDTLKQERCGVLAGHDNRVSCLGVTEDGMAVCTGSWDSFLRI 130
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BLAST of mRNA_M-pyrifera_M_contig102209.472.1 vs. uniprot
Match: A0A6J1VVW2_9SAUR (guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1-like n=2 Tax=Tetrapoda TaxID=32523 RepID=A0A6J1VVW2_9SAUR)

HSP 1 Score: 167 bits (424), Expect = 5.060e-51
Identity = 74/128 (57.81%), Postives = 92/128 (71.88%), Query Frame = 1 
Query:    1 WDLRSGRAQQTFLGHTEDINCCQYFPNGMAFGTGSDDHSCRLFDIRADRELIKYQSQGVPKGVTAIAFSISGRYLFASYDDTTCHVWDSLKGENVGDLKGHSQRVSCLGVSADGYALGTGSWDGTLKV 384
            WD+R   ++QTF GH  DIN   +FPNG AF TGSDD +CRLFD+RAD+EL+ Y    +  G+T++AFS SGR L A YDD  C++WDS+KG+  G L GH  RVSCLGV+ DG A+ TGSWD  LKV
Sbjct:   24 WDVRDSMSRQTFTGHESDINAVCFFPNGNAFTTGSDDATCRLFDLRADQELMLYSHDNIICGITSVAFSRSGRLLLAGYDDFNCNIWDSMKGDRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKV 151
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BLAST of mRNA_M-pyrifera_M_contig102209.472.1 vs. uniprot
Match: A0A163K906_ABSGL (Uncharacterized protein n=2 Tax=Absidia TaxID=4828 RepID=A0A163K906_ABSGL)

HSP 1 Score: 172 bits (436), Expect = 1.030e-50
Identity = 78/122 (63.93%), Postives = 90/122 (73.77%), Query Frame = 1 
Query:   19 RAQQTFLGHTEDINCCQYFPNGMAFGTGSDDHSCRLFDIRADRELIKYQSQGVPKGVTAIAFSISGRYLFASYDDTTCHVWDSLKGENVGDLKGHSQRVSCLGVSADGYALGTGSWDGTLKV 384
            +  QTF+GH  DIN  Q+FPNG AF TGSDD SCRLFD+RAD EL  Y S+ V  G+T+I FS+SGR LF  YDD  CHVWD+LKGE +G L  H  RVSCLGV+ DG AL TGSWD TLK+
Sbjct:  198 KCVQTFVGHESDINAVQFFPNGDAFATGSDDASCRLFDLRADCELSVYSSEAVLCGITSIGFSVSGRLLFGGYDDCNCHVWDTLKGERLGTLSAHDNRVSCLGVATDGMALCTGSWDSTLKI 319
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BLAST of mRNA_M-pyrifera_M_contig102209.472.1 vs. uniprot
Match: C7E3P4_RHIST (G-protein beta subunit n=2 Tax=Rhizopus TaxID=4842 RepID=C7E3P4_RHIST)

HSP 1 Score: 172 bits (437), Expect = 1.440e-50
Identity = 82/128 (64.06%), Postives = 92/128 (71.88%), Query Frame = 1 
Query:    1 WDLRSGRAQQTFLGHTEDINCCQYFPNGMAFGTGSDDHSCRLFDIRADRELIKYQSQGVPKGVTAIAFSISGRYLFASYDDTTCHVWDSLKGENVGDLKGHSQRVSCLGVSADGYALGTGSWDGTLKV 384
            WD+R  +  QTFLGH  DIN  Q+FP+G A GTGSDD SCRLFD+RADREL  Y  + +  GVT+I FS SGR LF  YDD +  VWD+LKGE VG L GH  RVSCLGVS DG AL TGSWD  LKV
Sbjct:  219 WDIRLKKCVQTFLGHESDINSVQFFPSGTAIGTGSDDASCRLFDLRADRELNTYAKEDLLHGVTSIDFSASGRLLFGGYDDYSTQVWDTLKGERVGVLAGHDNRVSCLGVSKDGMALCTGSWDSLLKV 346
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BLAST of mRNA_M-pyrifera_M_contig102209.472.1 vs. uniprot
Match: A0A168SK30_ABSGL (Uncharacterized protein n=3 Tax=Mucorales TaxID=4827 RepID=A0A168SK30_ABSGL)

HSP 1 Score: 176 bits (445), Expect = 1.950e-50
Identity = 81/128 (63.28%), Postives = 92/128 (71.88%), Query Frame = 1 
Query:    1 WDLRSGRAQQTFLGHTEDINCCQYFPNGMAFGTGSDDHSCRLFDIRADRELIKYQSQGVPKGVTAIAFSISGRYLFASYDDTTCHVWDSLKGENVGDLKGHSQRVSCLGVSADGYALGTGSWDGTLKV 384
            WD R+ R  QTF+GH  DIN  Q+FPNG AF TGSDD SCRLFD+RAD EL  Y    +  G+T+IAFS SGR LFA YDD  C VWD+LK E V  L GH  RVSCLGV++DG AL TGSWD TLK+
Sbjct:  358 WDTRTSRCVQTFVGHDSDINSVQFFPNGHAFATGSDDASCRLFDLRADCELEVYSDDSILSGITSIAFSSSGRLLFAGYDDYNCLVWDTLKNERVATLMGHDNRVSCLGVASDGMALCTGSWDSTLKI 485
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BLAST of mRNA_M-pyrifera_M_contig102209.472.1 vs. uniprot
Match: A0A7R8XEJ9_9CRUS (Hypothetical protein n=2 Tax=Pancrustacea TaxID=197562 RepID=A0A7R8XEJ9_9CRUS)

HSP 1 Score: 169 bits (428), Expect = 3.610e-50
Identity = 76/128 (59.38%), Postives = 90/128 (70.31%), Query Frame = 1 
Query:    1 WDLRSGRAQQTFLGHTEDINCCQYFPNGMAFGTGSDDHSCRLFDIRADRELIKYQSQGVPKGVTAIAFSISGRYLFASYDDTTCHVWDSLKGENVGDLKGHSQRVSCLGVSADGYALGTGSWDGTLKV 384
            WD+R G  +QTF GH  DIN   +FPNG AF TGSDD +CRLFDIRAD+EL  Y    +  G+T++AFS SGR L A YDD  C+VWDSL+ E  G L GH  RVSCLGV+ DG A+ TGSWD  LK+
Sbjct:  136 WDIRDGMCRQTFPGHESDINAVTFFPNGCAFATGSDDATCRLFDIRADQELAMYSHDNIICGITSVAFSKSGRLLLAGYDDFNCNVWDSLRAERAGVLAGHDNRVSCLGVTEDGMAVCTGSWDSFLKI 263
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BLAST of mRNA_M-pyrifera_M_contig102209.472.1 vs. uniprot
Match: A0A8H7BVZ7_9FUNG (Guanine nucleotide-binding protein subunit beta 1 n=1 Tax=Apophysomyces ossiformis TaxID=679940 RepID=A0A8H7BVZ7_9FUNG)

HSP 1 Score: 171 bits (432), Expect = 8.100e-50
Identity = 80/128 (62.50%), Postives = 91/128 (71.09%), Query Frame = 1 
Query:    1 WDLRSGRAQQTFLGHTEDINCCQYFPNGMAFGTGSDDHSCRLFDIRADRELIKYQSQGVPKGVTAIAFSISGRYLFASYDDTTCHVWDSLKGENVGDLKGHSQRVSCLGVSADGYALGTGSWDGTLKV 384
            WD+R  +  QTF GH  DIN  Q+FPNGMAFGTGSDD SCRLFD+RADREL  Y S+ +  G+T++AFS+SGR           HVWDSLKGE VG L  H  RVSCLGVS+DG AL TGSWD  LKV
Sbjct:  219 WDIRIQKCVQTFTGHETDINSVQFFPNGMAFGTGSDDASCRLFDLRADRELNSYASENILYGITSVAFSVSGRLXXXXXXXXXXHVWDSLKGERVGVLTSHENRVSCLGVSSDGMALCTGSWDSYLKV 346
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BLAST of mRNA_M-pyrifera_M_contig102209.472.1 vs. uniprot
Match: F9X1T0_ZYMTI (G-protein beta subunit n=137 Tax=Eukaryota TaxID=2759 RepID=F9X1T0_ZYMTI)

HSP 1 Score: 169 bits (429), Expect = 4.250e-49
Identity = 78/127 (61.42%), Postives = 90/127 (70.87%), Query Frame = 1 
Query:    1 WDLRSGRAQQTFLGHTEDINCCQYFPNGMAFGTGSDDHSCRLFDIRADRELIKYQSQGVPKGVTAIAFSISGRYLFASYDDTTCHVWDSLKGENVGDLKGHSQRVSCLGVSADGYALGTGSWDGTLK 381
            WD+R  +  QTF  H  DIN  Q+FPNG AFGTGSDD SCRLFDIRADREL  Y       G+T++AFS+SGR LFA YDD  C VWD L+GE VG L+GH  RVSCLGVS D  +L TGSWD  ++
Sbjct:  221 WDIRQQKCTQTFAAHDSDINAIQFFPNGHAFGTGSDDASCRLFDIRADRELQSYTIGEPVCGITSVAFSVSGRLLFAGYDDFECKVWDVLRGERVGTLQGHDNRVSCLGVSNDAMSLCTGSWDSMVR 347
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BLAST of mRNA_M-pyrifera_M_contig102209.472.1 vs. uniprot
Match: A0A3P7LFX2_DIBLA (Uncharacterized protein n=1 Tax=Dibothriocephalus latus TaxID=60516 RepID=A0A3P7LFX2_DIBLA)

HSP 1 Score: 162 bits (409), Expect = 8.530e-49
Identity = 71/128 (55.47%), Postives = 91/128 (71.09%), Query Frame = 1 
Query:    1 WDLRSGRAQQTFLGHTEDINCCQYFPNGMAFGTGSDDHSCRLFDIRADRELIKYQSQGVPKGVTAIAFSISGRYLFASYDDTTCHVWDSLKGENVGDLKGHSQRVSCLGVSADGYALGTGSWDGTLKV 384
            WD+R G+ +QTF GH  DIN   +FP+G AFGTGSDD +CRLFDIRAD+E+  +    +  G+T++AFS SGR L   YDD  C+VWD+LK +  G L GH  RVSCLGV+ DG A+ TGSWD  L++
Sbjct:   20 WDIRDGQCKQTFPGHESDINAITFFPSGYAFGTGSDDATCRLFDIRADQEVGMFSHDNIICGITSVAFSKSGRLLLGGYDDFNCNVWDTLKQDRAGVLAGHDNRVSCLGVTEDGMAVCTGSWDSFLRI 147
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig102209.472.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A2P6NK30_9EUKA7.520e-5766.41G protein b-subunit n=1 Tax=Planoprotostelium fung... [more]
A0A183VVZ2_TRIRE2.610e-5157.81WD_REPEATS_REGION domain-containing protein n=2 Ta... [more]
A0A6J1VVW2_9SAUR5.060e-5157.81guanine nucleotide-binding protein G(I)/G(S)/G(T) ... [more]
A0A163K906_ABSGL1.030e-5063.93Uncharacterized protein n=2 Tax=Absidia TaxID=4828... [more]
C7E3P4_RHIST1.440e-5064.06G-protein beta subunit n=2 Tax=Rhizopus TaxID=4842... [more]
A0A168SK30_ABSGL1.950e-5063.28Uncharacterized protein n=3 Tax=Mucorales TaxID=48... [more]
A0A7R8XEJ9_9CRUS3.610e-5059.38Hypothetical protein n=2 Tax=Pancrustacea TaxID=19... [more]
A0A8H7BVZ7_9FUNG8.100e-5062.50Guanine nucleotide-binding protein subunit beta 1 ... [more]
F9X1T0_ZYMTI4.250e-4961.42G-protein beta subunit n=137 Tax=Eukaryota TaxID=2... [more]
A0A3P7LFX2_DIBLA8.530e-4955.47Uncharacterized protein n=1 Tax=Dibothriocephalus ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig102209contigM-pyrifera_M_contig102209:2..385 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeFungi
Seed ortholog score172.2
Seed ortholog evalue1.6e-40
Seed eggNOG ortholog430998.XP_007674737.1
Preferred nametgb1
KEGG koko:K04536
KEGG Pathwayko04011,ko04014,ko04062,ko04151,ko04371,ko04713,ko04723,ko04724,ko04725,ko04726,ko04727,ko04728,ko04740,ko04744,ko04926,ko05032,ko05034,ko05167,ko05200,map04011,map04014,map04062,map04151,map04371,map04713,map04723,map04724,map04725,map04726,map04727,map04728,map04740,map04744,map04926,map05032,map05034,map05167,map05200
Hectar predicted targeting categoryother localisation
GOsGO:0000429,GO:0000430,GO:0000433,GO:0000749,GO:0000750,GO:0001403,GO:0001678,GO:0003674,GO:0005198,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005834,GO:0005886,GO:0005937,GO:0006355,GO:0006357,GO:0007154,GO:0007165,GO:0007186,GO:0007187,GO:0007188,GO:0007189,GO:0007584,GO:0008150,GO:0008277,GO:0009605,GO:0009606,GO:0009743,GO:0009746,GO:0009749,GO:0009756,GO:0009757,GO:0009889,GO:0009890,GO:0009892,GO:0009898,GO:0009966,GO:0009987,GO:0009991,GO:0010033,GO:0010182,GO:0010255,GO:0010468,GO:0010515,GO:0010525,GO:0010528,GO:0010556,GO:0010558,GO:0010605,GO:0010619,GO:0010629,GO:0010646,GO:0016020,GO:0019219,GO:0019222,GO:0019236,GO:0019725,GO:0019897,GO:0019898,GO:0019932,GO:0019933,GO:0019935,GO:0023051,GO:0023052,GO:0030159,GO:0030447,GO:0031135,GO:0031136,GO:0031137,GO:0031138,GO:0031139,GO:0031234,GO:0031323,GO:0031324,GO:0031326,GO:0031327,GO:0031667,GO:0031668,GO:0031669,GO:0031670,GO:0032005,GO:0032092,GO:0032947,GO:0032991,GO:0033500,GO:0034284,GO:0035556,GO:0036267,GO:0040007,GO:0042221,GO:0042592,GO:0042593,GO:0042995,GO:0043393,GO:0043577,GO:0043900,GO:0043901,GO:0043902,GO:0044093,GO:0044182,GO:0044424,GO:0044425,GO:0044459,GO:0044464,GO:0045013,GO:0045014,GO:0045892,GO:0045934,GO:0045990,GO:0046015,GO:0046999,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048583,GO:0048878,GO:0050789,GO:0050794,GO:0050896,GO:0051098,GO:0051099,GO:0051171,GO:0051172,GO:0051252,GO:0051253,GO:0051716,GO:0055082,GO:0060255,GO:0061984,GO:0061985,GO:0061986,GO:0061987,GO:0065007,GO:0065008,GO:0065009,GO:0070783,GO:0070887,GO:0071310,GO:0071322,GO:0071326,GO:0071331,GO:0071333,GO:0071444,GO:0071496,GO:0071944,GO:0080090,GO:0098552,GO:0098562,GO:0098796,GO:0098797,GO:0120025,GO:1901700,GO:1901701,GO:1902494,GO:1902679,GO:1903506,GO:1903507,GO:1905360,GO:2000112,GO:2000113,GO:2000241,GO:2000242,GO:2000243,GO:2001141
EggNOG free text desc.WD domain, G-beta repeat
EggNOG OGs1ZXBI@147541,38C52@33154,3MK18@451867,3NUGZ@4751,3QRC7@4890,KOG0286@1,KOG0286@2759
COG Functional cat.S
Best tax levelDothideomycetidae
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko04031,ko04147
Exons1
Model size384
Cds size384
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815522.8153722-CDS-M-pyrifera_M_contig102209:1..3851622815522.8153722-CDS-M-pyrifera_M_contig102209:1..385Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig102209 2..385 +
1692277448.7387033-CDS-M-pyrifera_M_contig102209:1..3851692277448.7387033-CDS-M-pyrifera_M_contig102209:1..385Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig102209 2..385 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig102209.472.1prot_M-pyrifera_M_contig102209.472.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig102209 2..385 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig102209.472.1

>prot_M-pyrifera_M_contig102209.472.1 ID=prot_M-pyrifera_M_contig102209.472.1|Name=mRNA_M-pyrifera_M_contig102209.472.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=128bp
WDLRSGRAQQTFLGHTEDINCCQYFPNGMAFGTGSDDHSCRLFDIRADRE
LIKYQSQGVPKGVTAIAFSISGRYLFASYDDTTCHVWDSLKGENVGDLKG
HSQRVSCLGVSADGYALGTGSWDGTLKV
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mRNA from alignment at M-pyrifera_M_contig102209:2..385+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig102209.472.1 ID=mRNA_M-pyrifera_M_contig102209.472.1|Name=mRNA_M-pyrifera_M_contig102209.472.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=384bp|location=Sequence derived from alignment at M-pyrifera_M_contig102209:2..385+ (Macrocystis pyrifera P11B4 male)
TGGGATCTCCGCTCCGGAAGGGCCCAGCAGACCTTCCTCGGTCACACTGA
GGACATCAACTGCTGCCAGTACTTCCCCAACGGCATGGCCTTCGGAACTG
GCTCTGACGACCACTCGTGCCGCCTCTTCGATATCCGCGCCGACCGCGAG
CTCATCAAGTACCAGTCGCAGGGCGTCCCCAAGGGTGTCACTGCCATCGC
CTTCTCCATCAGCGGAAGGTACCTCTTCGCTTCTTACGACGACACCACGT
GCCATGTCTGGGATTCGCTGAAGGGCGAGAACGTGGGTGATCTCAAGGGC
CACTCGCAGCGTGTCAGCTGCTTGGGTGTGTCAGCGGACGGATACGCCTT
GGGAACAGGAAGCTGGGACGGCACCCTGAAGGTT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig102209:2..385+

>mRNA_M-pyrifera_M_contig102209.472.1 ID=mRNA_M-pyrifera_M_contig102209.472.1|Name=mRNA_M-pyrifera_M_contig102209.472.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=768bp|location=Sequence derived from alignment at M-pyrifera_M_contig102209:2..385+ (Macrocystis pyrifera P11B4 male)
TGGGATCTCCGCTCCGGAAGGGCCCAGCAGACCTTCCTCGGTCACACTGA
GGACATCAACTGCTGCCAGTACTTCCCCAACGGCATGGCCTTCGGAACTG
GCTCTGACGACCACTCGTGCCGCCTCTTCGATATCCGCGCCGACCGCGAG
CTCATCAAGTACCAGTCGCAGGGCGTCCCCAAGGGTGTCACTGCCATCGC
CTTCTCCATCAGCGGAAGGTACCTCTTCGCTTCTTACGACGACACCACGT
GCCATGTCTGGGATTCGCTGAAGGGCGAGAACGTGGGTGATCTCAAGGGC
CACTCGCAGCGTGTCAGCTGCTTGGGTGTGTCAGCGGACGGATACGCCTT
GGGAACAGGAAGCTGGGACGGCACCCTGAAGGTTTGGGATCTCCGCTCCG
GAAGGGCCCAGCAGACCTTCCTCGGTCACACTGAGGACATCAACTGCTGC
CAGTACTTCCCCAACGGCATGGCCTTCGGAACTGGCTCTGACGACCACTC
GTGCCGCCTCTTCGATATCCGCGCCGACCGCGAGCTCATCAAGTACCAGT
CGCAGGGCGTCCCCAAGGGTGTCACTGCCATCGCCTTCTCCATCAGCGGA
AGGTACCTCTTCGCTTCTTACGACGACACCACGTGCCATGTCTGGGATTC
GCTGAAGGGCGAGAACGTGGGTGATCTCAAGGGCCACTCGCAGCGTGTCA
GCTGCTTGGGTGTGTCAGCGGACGGATACGCCTTGGGAACAGGAAGCTGG
GACGGCACCCTGAAGGTT
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