mRNA_M-pyrifera_M_contig102128.459.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig102128.459.1
Unique NamemRNA_M-pyrifera_M_contig102128.459.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig102128.459.1 vs. uniprot
Match: A0A812UZ52_SYMMI (Ddx10 protein n=1 Tax=Symbiodinium microadriaticum TaxID=2951 RepID=A0A812UZ52_SYMMI)

HSP 1 Score: 182 bits (463), Expect = 4.430e-56
Identity = 95/156 (60.90%), Postives = 120/156 (76.92%), Query Frame = 1
Query:    1 GLERSGFTKLTEVQRAAIPHALVGRDVLGAAKTGSGKTLAFLVPVLERMFRSRRTGGDGLGALIISPTRELARQIMDVLRRVGKMHKGIVGGVVTGGV-HFEREKREVGGLTVLVGTPGRLLQHFEQSPDLDLTSLEMLVLDEADRLLDMGFRAQL 465
            GL ++G +  T++Q AA+PHAL GRDVLGAAKTGSGKTL+F++PVLE ++R R   GDGL A+II+PTRELA QI DVLR  G+ H+ I  G+VTGG   FE E+  +  + +LV TPGRLLQHFEQ+P  D ++L  LVLDEADR+LDMGF+ QL
Sbjct:    3 GLVKAGISVPTDIQAAALPHALCGRDVLGAAKTGSGKTLSFVIPVLELLYRERWGLGDGLAAIIITPTRELALQIFDVLRTTGRKHQ-ISAGLVTGGKKEFEEEQERIVKMNILVATPGRLLQHFEQTPGFDCSNLLALVLDEADRILDMGFKHQL 157          
BLAST of mRNA_M-pyrifera_M_contig102128.459.1 vs. uniprot
Match: A0A5A8DZA9_CAFRO (Uncharacterized protein n=5 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8DZA9_CAFRO)

HSP 1 Score: 193 bits (490), Expect = 9.750e-55
Identity = 97/150 (64.67%), Postives = 118/150 (78.67%), Query Frame = 1
Query:    4 LERSGFTKLTEVQRAAIPHALVGRDVLGAAKTGSGKTLAFLVPVLERMFRSRRTGGDGLGALIISPTRELARQIMDVLRRVGKMHKGIVGGVVTGGVHFEREKREVGGLTVLVGTPGRLLQHFEQSPDLDLTSLEMLVLDEADRLLDMGF 453
            L  + +TKLT+VQRAA+PHAL GRDVLGAAKTGSGKTLAFLVP++E +FR R +  DG+GA+II+PTRELA QIM  LRR+G  H  +  G++ GG  F  E+  +G L+V+V TPGRL QH EQSP+LD +SL  LVLDEADRLLDMGF
Sbjct:   28 LTSNKWTKLTDVQRAAVPHALAGRDVLGAAKTGSGKTLAFLVPIVELLFRERWSAEDGVGAIIITPTRELALQIMQALRRLGTRHPDLTAGLLCGGKAFSEEQAVLGRLSVVVATPGRLQQHLEQSPELDTSSLRALVLDEADRLLDMGF 177          
BLAST of mRNA_M-pyrifera_M_contig102128.459.1 vs. uniprot
Match: A0A2R5GJM3_9STRA (ATP-dependent RNA helicase dbp4 n=1 Tax=Hondaea fermentalgiana TaxID=2315210 RepID=A0A2R5GJM3_9STRA)

HSP 1 Score: 193 bits (491), Expect = 1.680e-54
Identity = 100/156 (64.10%), Postives = 122/156 (78.21%), Query Frame = 1
Query:    1 GLERSGFTKLTEVQRAAIPHALVGRDVLGAAKTGSGKTLAFLVPVLERMFRSRRTGGDGLGALIISPTRELARQIMDVLRRVGKMHKGIVGGVVTGGVHFEREKREVGGLTVLVGTPGRLLQHFEQSPDLDLTSLEMLVLDEADRLLDMGFRAQLS 468
            GLER  F  +T++Q+ AIPHALVGRDVLGAA+TGSGKTLAF+VP+LER+FR   T G GLGA+IISPTRELA QI +VLR VG  H G   G+V GG  F RE+  V  L V++ TPGRLLQH EQ+P +D + ++MLVLDEADRLLD+GF+ QL+
Sbjct:  106 GLERGKFVTMTKIQQLAIPHALVGRDVLGAARTGSGKTLAFVVPLLERLFRLGWTPGLGLGAVIISPTRELALQIYEVLRVVGYRH-GYTAGIVMGGSDFRREQANVHQLAVMIATPGRLLQHLEQTPTMDASQVQMLVLDEADRLLDLGFKKQLT 260          
BLAST of mRNA_M-pyrifera_M_contig102128.459.1 vs. uniprot
Match: F0Y9C4_AURAN (Uncharacterized protein (Fragment) n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0Y9C4_AURAN)

HSP 1 Score: 186 bits (472), Expect = 5.240e-54
Identity = 95/154 (61.69%), Postives = 112/154 (72.73%), Query Frame = 1
Query:    1 GLERSGFTKLTEVQRAAIPHALVGRDVLGAAKTGSGKTLAFLVPVLERMFRSRRTGGDGLGALIISPTRELARQIMDVLRRVGKMHKGIVGGVVTGGVHFEREKREVGGLTVLVGTPGRLLQHFEQSPDLDLTSLEMLVLDEADRLLDMGFRAQ 462
            GL   GF  +  +QR AIPHAL GRDVLGAA+TGSGKTLAFLVP LE ++R R + GDGLGALIISPTRELA QI DVLR+ G  H    G +  GG  F+ E+  VG + +LV TPGR+LQH E+SP  D T L  LV+DEADR++DMGF AQ
Sbjct:   47 GLTSGGFETMKPIQRCAIPHALAGRDVLGAARTGSGKTLAFLVPALEALYRHRWSAGDGLGALIISPTRELAAQIFDVLRKAGSCHDVSAGLITGGGRDFDAERDRVGRMCLLVATPGRVLQHAEESPAFDATGLLALVVDEADRVVDMGFAAQ 200          
BLAST of mRNA_M-pyrifera_M_contig102128.459.1 vs. uniprot
Match: A0A024GKP7_9STRA (Uncharacterized protein n=1 Tax=Albugo candida TaxID=65357 RepID=A0A024GKP7_9STRA)

HSP 1 Score: 192 bits (488), Expect = 5.610e-54
Identity = 93/156 (59.62%), Postives = 125/156 (80.13%), Query Frame = 1
Query:    1 GLERSGFTKLTEVQRAAIPHALVGRDVLGAAKTGSGKTLAFLVPVLERMFRSRRTGGDGLGALIISPTRELARQIMDVLRRVGKMHKGIVGGVVTGGVHFEREKREVGGLTVLVGTPGRLLQHFEQSPDLDLTSLEMLVLDEADRLLDMGFRAQLS 468
            GL++   TK T++Q  AIPHAL GRD+LGAAKTGSGKTLAFL+P+LE+++RSR    +GLGALIISPTRELA QI +VLR +GK H     G++ GG +F+ E+  +G + ++V TPGRLLQH EQ+P+ D+++L+MLVLDEADR++DMGF AQ++
Sbjct:  275 GLQKCKLTKPTKIQMGAIPHALAGRDILGAAKTGSGKTLAFLIPLLEKLYRSRWDSSEGLGALIISPTRELALQIFEVLRSIGKTHS-FSAGLIIGGKNFKEEQFRLGKMNIIVCTPGRLLQHLEQTPNFDVSTLQMLVLDEADRIMDMGFSAQVN 429          
BLAST of mRNA_M-pyrifera_M_contig102128.459.1 vs. uniprot
Match: A0A2D4BS33_PYTIN (Uncharacterized protein n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4BS33_PYTIN)

HSP 1 Score: 192 bits (487), Expect = 9.020e-54
Identity = 97/156 (62.18%), Postives = 122/156 (78.21%), Query Frame = 1
Query:    1 GLERSGFTKLTEVQRAAIPHALVGRDVLGAAKTGSGKTLAFLVPVLERMFRSRRTGGDGLGALIISPTRELARQIMDVLRRVGKMHKGIVGGVVTGGVHFEREKREVGGLTVLVGTPGRLLQHFEQSPDLDLTSLEMLVLDEADRLLDMGFRAQLS 468
            GL + GFTK T +Q  AIPHAL GRD+LGAAKTGSGKTLAFL+P+LE+++R R    DGLGAL+ISPTRELA QI +VLR VGK H     G+V GG +F+ E+  +  + +LV TPGR+LQH EQ+PD D T++++LVLDEADR+LDMGF+ QLS
Sbjct:  284 GLAKCGFTKPTRIQIGAIPHALAGRDILGAAKTGSGKTLAFLLPLLEKLYRLRWDVEDGLGALVISPTRELALQIFEVLRSVGKFHS-FSAGLVIGGKNFKEEQYRLVRMNILVCTPGRMLQHMEQTPDFDATNVQLLVLDEADRILDMGFQQQLS 438          
BLAST of mRNA_M-pyrifera_M_contig102128.459.1 vs. uniprot
Match: A0A3R7ZII9_9STRA (Helicase ATP-binding domain-containing protein n=2 Tax=Aphanomyces astaci TaxID=112090 RepID=A0A3R7ZII9_9STRA)

HSP 1 Score: 181 bits (459), Expect = 1.040e-53
Identity = 90/156 (57.69%), Postives = 119/156 (76.28%), Query Frame = 1
Query:    1 GLERSGFTKLTEVQRAAIPHALVGRDVLGAAKTGSGKTLAFLVPVLERMFRSRRTGGDGLGALIISPTRELARQIMDVLRRVGKMHKGIVGGVVTGGVHFEREKREVGGLTVLVGTPGRLLQHFEQSPDLDLTSLEMLVLDEADRLLDMGFRAQLS 468
            GL+R  F K T++Q   IPHAL GRD+L AAKTGSGKTLAFL+P+LE++FR R    DG+GAL+ISPTRELA QI +VLR +GK H     G+V GG +F+ E+  +  + +LV TPGRLLQH EQ+ + +L++L++L+LDEADR+LDMGF  QL+
Sbjct:   50 GLKRGKFAKPTKIQIGTIPHALAGRDILAAAKTGSGKTLAFLIPMLEKLFRQRWDVEDGVGALVISPTRELAMQIFEVLRSIGKSHT-FSAGLVIGGKNFQEEQYRIIKMNILVATPGRLLQHMEQTANFELSNLQVLILDEADRILDMGFSNQLT 204          
BLAST of mRNA_M-pyrifera_M_contig102128.459.1 vs. uniprot
Match: A0A7S1G8S0_9STRA (Hypothetical protein (Fragment) n=1 Tax=Bicosoecida sp. CB-2014 TaxID=1486930 RepID=A0A7S1G8S0_9STRA)

HSP 1 Score: 177 bits (448), Expect = 2.710e-53
Identity = 87/147 (59.18%), Postives = 113/147 (76.87%), Query Frame = 1
Query:    1 GLERSGFTKLTEVQRAAIPHALVGRDVLGAAKTGSGKTLAFLVPVLERMFRSRRTGGDGLGALIISPTRELARQIMDVLRRVGKMHKGIVGGVVTGGVHFEREKREVGGLTVLVGTPGRLLQHFEQSPDLDLTSLEMLVLDEADRLL 441
            GL ++ F ++T++QR AIPH L GRDVLGAAKTGSGKTLAFL+PV+E ++R+R +  DGLGA++ISPTRELA QI +VLR V   H G+  G VTGG  F+ E+  +  L ++V TPGRLLQH EQ+P  D + +++LVLDEADRLL
Sbjct:   74 GLAKAKFKRMTQIQRVAIPHGLAGRDVLGAAKTGSGKTLAFLIPVVEALYRARWSREDGLGAIVISPTRELALQIFEVLRAVAGSHFGLTAGFVTGGKPFQEEQYAMSKLNIIVCTPGRLLQHMEQTPGFDASLVKVLVLDEADRLL 220          
BLAST of mRNA_M-pyrifera_M_contig102128.459.1 vs. uniprot
Match: A0A0G4H3S2_9ALVE (Uncharacterized protein n=1 Tax=Chromera velia CCMP2878 TaxID=1169474 RepID=A0A0G4H3S2_9ALVE)

HSP 1 Score: 190 bits (483), Expect = 3.220e-53
Identity = 98/156 (62.82%), Postives = 117/156 (75.00%), Query Frame = 1
Query:    1 GLERSGFTKLTEVQRAAIPHALVGRDVLGAAKTGSGKTLAFLVPVLERMFRSRRTGGDGLGALIISPTRELARQIMDVLRRVGKMHKGIVGGVVTGGVHFEREKREVGGLTVLVGTPGRLLQHFEQSPDLDLTSLEMLVLDEADRLLDMGFRAQLS 468
            GL+  GF +LT +Q+ AIPHAL GRDVLG AKTGSGKTLAF+VP++ER+FR R T  DGLGALI+SPTRELA QI DVLR VGK H  +  G V GG     E+  +  L VLV TPGRLLQH ++SP  D ++L++LVLDEADR LD+GFR  LS
Sbjct:  142 GLKAGGFVQLTPIQKEAIPHALAGRDVLGEAKTGSGKTLAFIVPIVERLFRQRFTSTDGLGALILSPTRELAVQIFDVLRNVGKQHD-LSAGCVIGGKDVSAEQHRIHTLNVLVATPGRLLQHMDESPSWDASNLQILVLDEADRCLDLGFRDTLS 296          
BLAST of mRNA_M-pyrifera_M_contig102128.459.1 vs. uniprot
Match: A0A485KZ34_9STRA (Aste57867_13805 protein n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485KZ34_9STRA)

HSP 1 Score: 188 bits (477), Expect = 4.270e-53
Identity = 94/156 (60.26%), Postives = 122/156 (78.21%), Query Frame = 1
Query:    1 GLERSGFTKLTEVQRAAIPHALVGRDVLGAAKTGSGKTLAFLVPVLERMFRSRRTGGDGLGALIISPTRELARQIMDVLRRVGKMHKGIVGGVVTGGVHFEREKREVGGLTVLVGTPGRLLQHFEQSPDLDLTSLEMLVLDEADRLLDMGFRAQLS 468
            GL+R  F K T++Q   IPHALVGRD+L AAKTGSGKTLAFL+P+LE+++R+R    DG+GALIISPTRELA QI +VLR +GK H     G+V GG +F+ E+  +  + +LV TPGRLLQH EQ+P+ +L++L ML+LDEADR+LDMGF AQL+
Sbjct:   50 GLKRGKFAKPTKIQIGTIPHALVGRDILAAAKTGSGKTLAFLIPMLEKLYRNRWDTEDGVGALIISPTRELAMQIFEVLRSIGKSHS-FSAGLVIGGKNFQEEQYRIIKMNILVATPGRLLQHMEQTPNFELSNLLMLILDEADRILDMGFSAQLT 204          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig102128.459.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A812UZ52_SYMMI4.430e-5660.90Ddx10 protein n=1 Tax=Symbiodinium microadriaticum... [more]
A0A5A8DZA9_CAFRO9.750e-5564.67Uncharacterized protein n=5 Tax=Cafeteria roenberg... [more]
A0A2R5GJM3_9STRA1.680e-5464.10ATP-dependent RNA helicase dbp4 n=1 Tax=Hondaea fe... [more]
F0Y9C4_AURAN5.240e-5461.69Uncharacterized protein (Fragment) n=1 Tax=Aureoco... [more]
A0A024GKP7_9STRA5.610e-5459.62Uncharacterized protein n=1 Tax=Albugo candida Tax... [more]
A0A2D4BS33_PYTIN9.020e-5462.18Uncharacterized protein n=1 Tax=Pythium insidiosum... [more]
A0A3R7ZII9_9STRA1.040e-5357.69Helicase ATP-binding domain-containing protein n=2... [more]
A0A7S1G8S0_9STRA2.710e-5359.18Hypothetical protein (Fragment) n=1 Tax=Bicosoecid... [more]
A0A0G4H3S2_9ALVE3.220e-5362.82Uncharacterized protein n=1 Tax=Chromera velia CCM... [more]
A0A485KZ34_9STRA4.270e-5360.26Aste57867_13805 protein n=1 Tax=Aphanomyces stella... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig102128contigM-pyrifera_M_contig102128:5..472 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score190.3
Seed ortholog evalue6.9e-46
Seed eggNOG ortholog2880.D8LH86
KEGG koko:K14776
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003724,GO:0003824,GO:0004004,GO:0004386,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005730,GO:0005737,GO:0006139,GO:0006725,GO:0006807,GO:0008026,GO:0008150,GO:0008152,GO:0008186,GO:0009987,GO:0010501,GO:0016070,GO:0016462,GO:0016787,GO:0016817,GO:0016818,GO:0016887,GO:0017111,GO:0031974,GO:0031981,GO:0034641,GO:0042623,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0044237,GO:0044238,GO:0044422,GO:0044424,GO:0044428,GO:0044446,GO:0044464,GO:0046483,GO:0070013,GO:0070035,GO:0071704,GO:0090304,GO:0140098,GO:1901360
EggNOG free text desc.box C/D snoRNA binding
EggNOG OGsCOG0513@1,KOG0343@2759
EC3.6.4.13
COG Functional cat.JKL
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko03009
Exons1
Model size468
Cds size468
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815522.2526875-CDS-M-pyrifera_M_contig102128:4..4721622815522.2526875-CDS-M-pyrifera_M_contig102128:4..472Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig102128 5..472 -
1692277448.5160542-CDS-M-pyrifera_M_contig102128:4..4721692277448.5160542-CDS-M-pyrifera_M_contig102128:4..472Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig102128 5..472 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig102128.459.1prot_M-pyrifera_M_contig102128.459.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig102128 5..472 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig102128.459.1

>prot_M-pyrifera_M_contig102128.459.1 ID=prot_M-pyrifera_M_contig102128.459.1|Name=mRNA_M-pyrifera_M_contig102128.459.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=156bp
GLERSGFTKLTEVQRAAIPHALVGRDVLGAAKTGSGKTLAFLVPVLERMF
RSRRTGGDGLGALIISPTRELARQIMDVLRRVGKMHKGIVGGVVTGGVHF
EREKREVGGLTVLVGTPGRLLQHFEQSPDLDLTSLEMLVLDEADRLLDMG
FRAQLS
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mRNA from alignment at M-pyrifera_M_contig102128:5..472-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig102128.459.1 ID=mRNA_M-pyrifera_M_contig102128.459.1|Name=mRNA_M-pyrifera_M_contig102128.459.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=468bp|location=Sequence derived from alignment at M-pyrifera_M_contig102128:5..472- (Macrocystis pyrifera P11B4 male)
GGCTTGGAGCGAAGCGGGTTCACCAAGCTGACGGAGGTGCAGCGCGCAGC CATCCCCCACGCTCTGGTGGGCCGTGACGTGCTTGGGGCGGCGAAAACGG GGAGTGGGAAGACTTTGGCGTTTTTAGTGCCGGTTCTGGAGCGAATGTTC CGTTCTCGTCGCACTGGTGGGGATGGTCTGGGCGCGTTGATTATTTCTCC GACCAGAGAATTGGCGCGGCAGATTATGGATGTACTTCGGCGCGTTGGGA AAATGCACAAGGGCATTGTAGGCGGGGTTGTGACTGGAGGGGTGCATTTT GAGCGCGAGAAACGGGAGGTGGGGGGGCTGACGGTGTTGGTGGGCACTCC AGGGAGGCTGTTGCAGCATTTTGAGCAATCTCCAGATTTGGACCTCACGT CTCTGGAAATGCTCGTCTTGGATGAAGCTGATCGGCTCCTTGATATGGGC TTCCGAGCACAGCTCTCC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig102128:5..472-

>mRNA_M-pyrifera_M_contig102128.459.1 ID=mRNA_M-pyrifera_M_contig102128.459.1|Name=mRNA_M-pyrifera_M_contig102128.459.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=936bp|location=Sequence derived from alignment at M-pyrifera_M_contig102128:5..472- (Macrocystis pyrifera P11B4 male)
GGCTTGGAGCGAAGCGGGTTCACCAAGCTGACGGAGGTGCAGCGCGCAGC
CATCCCCCACGCTCTGGTGGGCCGTGACGTGCTTGGGGCGGCGAAAACGG
GGAGTGGGAAGACTTTGGCGTTTTTAGTGCCGGTTCTGGAGCGAATGTTC
CGTTCTCGTCGCACTGGTGGGGATGGTCTGGGCGCGTTGATTATTTCTCC
GACCAGAGAATTGGCGCGGCAGATTATGGATGTACTTCGGCGCGTTGGGA
AAATGCACAAGGGCATTGTAGGCGGGGTTGTGACTGGAGGGGTGCATTTT
GAGCGCGAGAAACGGGAGGTGGGGGGGCTGACGGTGTTGGTGGGCACTCC
AGGGAGGCTGTTGCAGCATTTTGAGCAATCTCCAGATTTGGACCTCACGT
CTCTGGAAATGCTCGTCTTGGATGAAGCTGATCGGCTCCTTGATATGGGC
TTCCGAGCACAGCTCTCCGGCTTGGAGCGAAGCGGGTTCACCAAGCTGAC
GGAGGTGCAGCGCGCAGCCATCCCCCACGCTCTGGTGGGCCGTGACGTGC
TTGGGGCGGCGAAAACGGGGAGTGGGAAGACTTTGGCGTTTTTAGTGCCG
GTTCTGGAGCGAATGTTCCGTTCTCGTCGCACTGGTGGGGATGGTCTGGG
CGCGTTGATTATTTCTCCGACCAGAGAATTGGCGCGGCAGATTATGGATG
TACTTCGGCGCGTTGGGAAAATGCACAAGGGCATTGTAGGCGGGGTTGTG
ACTGGAGGGGTGCATTTTGAGCGCGAGAAACGGGAGGTGGGGGGGCTGAC
GGTGTTGGTGGGCACTCCAGGGAGGCTGTTGCAGCATTTTGAGCAATCTC
CAGATTTGGACCTCACGTCTCTGGAAATGCTCGTCTTGGATGAAGCTGAT
CGGCTCCTTGATATGGGCTTCCGAGCACAGCTCTCC
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