mRNA_M-pyrifera_M_contig102007.435.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig102007.435.1
Unique NamemRNA_M-pyrifera_M_contig102007.435.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig102007.435.1 vs. uniprot
Match: A0A0G4I4J7_9ALVE (Fe2OG dioxygenase domain-containing protein n=1 Tax=Chromera velia CCMP2878 TaxID=1169474 RepID=A0A0G4I4J7_9ALVE)

HSP 1 Score: 145 bits (365), Expect = 3.360e-39
Identity = 75/177 (42.37%), Postives = 110/177 (62.15%), Query Frame = 1
Query:   58 ALDAPVGEVGCLC---ELVSKEEERWLMGMVDFEESRWQQLRHRRLQVYGGKVERDGVKDVEELPPWLGRIAKRLVDCGVFEADKMPNHVLVNDYAPGEGIAGHTDGPAYYPLVATLSLGSDAIMQFEPRVSSDVIGTPLHKSLLSTQSCEVLLQRRSVVIFKGDAYHNMLHSIPSR 579
            +L  P+G+V  +C      S+E E+ ++  +    SR+  LR RR QV+GG+V  +G++D E+LP +L  +A+ LV CG F+A+  PNHVLVN+Y PG GI  HTDGPAY+P VA LSLGS A+  F  + +    G     +    ++  V L RRS+++F  D Y + LHS+PS+
Sbjct:   49 SLPPPLGDVKTVCVVENFTSEEGEKTILSNISSSVSRFNPLRGRRTQVWGGQVTAEGLQDAEKLPQFLETLAEGLVSCGAFQAENKPNHVLVNEYEPGGGIMPHTDGPAYFPQVAILSLGSSAVFVFGRKATQTPSGDIGETAERPKETLRVFLPRRSLLLFSDDLYTDWLHSLPSQ 225          
BLAST of mRNA_M-pyrifera_M_contig102007.435.1 vs. uniprot
Match: A0A7S0PB53_CAFRO (Hypothetical protein n=1 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A7S0PB53_CAFRO)

HSP 1 Score: 143 bits (361), Expect = 2.030e-38
Identity = 75/150 (50.00%), Postives = 96/150 (64.00%), Query Frame = 1
Query:  157 RWQQLRHRRLQVYGGK-VERDGVKDVEELPPWLGRIAKRLVDCGVFEADKMPNHVLVNDYAPGEGIAGHTDGPAYYPLVATLSLGSDAIMQFEPRVSSDVIGTPLHKSLLSTQSCEVLLQRRSVVIFKGDAYHNMLHSIPS----RITEK 591
            RW +LR RRL  +GG  +    +   E LPPWLG +  RLV  G F  DK PNHVLVN Y PGEGI  HTDGP Y+P  ATLSLGSDA+M+F PRV S+++GT     L + +S +  L RRS+V+F  +AY + LH + +    R+T K
Sbjct:  127 RWVRLRGRRLLQFGGTPMPGPRLLGREPLPPWLGAVVDRLVATGAFPEDKRPNHVLVNAYGPGEGILPHTDGPLYFPCTATLSLGSDAVMRFAPRVPSELVGTSSQSHLEAAKSFDAGLARRSLVVFSDEAYTSALHDVAAVGEHRVTAK 276          
BLAST of mRNA_M-pyrifera_M_contig102007.435.1 vs. uniprot
Match: A0A7S2G3H8_9STRA (Hypothetical protein n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2G3H8_9STRA)

HSP 1 Score: 138 bits (347), Expect = 1.010e-37
Identity = 76/160 (47.50%), Postives = 101/160 (63.12%), Query Frame = 1
Query:  118 ERWLMGMVDFE-ESRWQQLRHRRLQVYGGKVERDGVKDVEELPPWLGRIAKRLVDCGVFEADKMPNHVLVNDYAPGEGIAGHTDGPAYYPLVATLSLGSDAIMQFEPRVSSDVIGTPLHKSLLSTQSCEVLLQRRSVVIFKGDAYHNMLHSIPSRITEKL 594
            E+ +M M  +  ES W  L  RR+Q +GG+         E LPPWL  I   LVD GVF ++  PNHVL+N+YA GEGI  H+DGP Y+P  ATLSLG  AIM FEPR+SS  IG      + S   CEV+L+ RS+V+F+  AY + +H+I SR T+++
Sbjct:    1 EKAIMDMTSYAIESPWISLSGRRVQCWGGQPNERTKFVPEPLPPWLVDICTALVDAGVFPSEVRPNHVLINEYANGEGILPHSDGPMYFPKTATLSLGGSAIMTFEPRISSGDIG-----EVSSDPICEVILRPRSLVVFEDAAYIDYMHAIASRQTDQV 155          
BLAST of mRNA_M-pyrifera_M_contig102007.435.1 vs. uniprot
Match: UPI001F4F4B1C (alpha-ketoglutarate-dependent dioxygenase alkB homolog 6 n=1 Tax=Schistocerca americana TaxID=7009 RepID=UPI001F4F4B1C)

HSP 1 Score: 138 bits (348), Expect = 2.000e-37
Identity = 67/166 (40.36%), Postives = 104/166 (62.65%), Query Frame = 1
Query:   76 GEVGCLCELVSKEEERWLMGMVD-FEESRWQQLRHRRLQVYGGKVERDGVKDVEELPPWLGRIAKRLVDCGVFEADKMPNHVLVNDYAPGEGIAGHTDGPAYYPLVATLSLGSDAIMQFEPRVSSDVIGTPLHKSLLSTQSCEVLLQRRSVVIFKGDAYHNMLHSI 570
            G V  + + +++EEER+++  VD   + +W QL HRRLQ +GG     G+   E++P WL +   ++   G+FE +K PNHVL+N+Y PG+GI  HTDGP +YP V T+S GS  +++F+P  S D  G  +        +  +LL++RS++I + + YHN LHSI
Sbjct:   13 GTVYYIPQFITEEEERYIIEHVDSVPKPKWTQLSHRRLQNWGGVPHPKGMI-AEKIPDWLKKYIDKIATLGIFETEKCPNHVLINEYLPGQGIMPHTDGPLFYPTVTTISCGSHTLLEFQPSKSEDCQGASVQDDASKGPTFSLLLEQRSLLILQDELYHNYLHSI 177          
BLAST of mRNA_M-pyrifera_M_contig102007.435.1 vs. uniprot
Match: UPI0014780CBA (alpha-ketoglutarate-dependent dioxygenase alkB homolog 6 n=3 Tax=Osmia TaxID=124287 RepID=UPI0014780CBA)

HSP 1 Score: 128 bits (321), Expect = 2.100e-33
Identity = 67/163 (41.10%), Postives = 99/163 (60.74%), Query Frame = 1
Query:  100 LVSKEEERWLMGMVDFEESRWQQLRHRRLQVYGGKVERDGVKDVEELPPWLGRIAKRLVDCGVFEADKMPNHVLVNDYAPGEGIAGHTDGPAYYPLVATLSLGSDAIMQFEPRVSSDVIGTPLHKSLLSTQSCEVLLQRRSVVIFKGDAYHNMLHSIPSRITE 588
            + S+EEE  +  + +    +W QL HRRLQ +GG     G+   EE+P WL +   ++  C VFE  K+PNHVL+N+Y PG+GI  H+DGP ++P+V T+S GS  +++F  R+ S    T  H+  L       LL+ RS++I +GD YHN LHSI  + T+
Sbjct:   27 VTSEEEEEIIKYVNNAPLPKWTQLSHRRLQNWGGIPHPKGMI-AEEIPSWLTKYVDKVSSCNVFEKGKLPNHVLINEYLPGQGIMAHSDGPLFHPIVTTISCGSHTLLEFYKRLDS----TQQHEQNLEFS---FLLEPRSLLILQGDLYHNYLHSIAEKDTD 181          
BLAST of mRNA_M-pyrifera_M_contig102007.435.1 vs. uniprot
Match: A0A7S2RZS8_9STRA (Hypothetical protein n=1 Tax=labyrinthulid quahog parasite QPX TaxID=96639 RepID=A0A7S2RZS8_9STRA)

HSP 1 Score: 129 bits (323), Expect = 2.360e-33
Identity = 71/188 (37.77%), Postives = 105/188 (55.85%), Query Frame = 1
Query:   31 PLPSYGERNALDAPVGEVGCLCELVSKEEERWLMGMVDFEESRWQQLRHRRLQVYGGKVERDGVKDVEELPPWLGRIAKRLVDCGVFEADKMPNHVLVNDYAPGEGIAGHTDGPAYYPLVATLSLGSDAIMQFEPRVSSDVIGTPLHKSLLSTQSCEVLLQRRSVVIFKGDAYHNMLHSIPSRITEKL 594
            P P++     +   +  V    + ++ ++    M  V  + ++W++L  RRLQ Y G            LPPW+  +A  LV CG+F  +  P+H L+N+Y PG+GI GHTDG AY PLVA LSLGS A++ F  R+++  IGT   K L     C++LLQ +S+++FK DAY   LH I    +E L
Sbjct:   51 PEPNWESTRRVKCDLDSVFYFQDWINDQDHDAFMEQVYCKAAKWKELPFRRLQCYQGNG----------LPPWIQNLADTLVRCGIFPVEIRPDHALLNEYLPGQGILGHTDGCAYLPLVACLSLGSPALISFRERLATHEIGTREPKLL-----CQLLLQPKSLIVFKADAYEYALHEICPTFSETL 223          
BLAST of mRNA_M-pyrifera_M_contig102007.435.1 vs. uniprot
Match: UPI0006154243 (alpha-ketoglutarate-dependent dioxygenase alkB homolog 6 n=1 Tax=Megachile rotundata TaxID=143995 RepID=UPI0006154243)

HSP 1 Score: 127 bits (320), Expect = 2.960e-33
Identity = 66/163 (40.49%), Postives = 99/163 (60.74%), Query Frame = 1
Query:  100 LVSKEEERWLMGMVDFEESRWQQLRHRRLQVYGGKVERDGVKDVEELPPWLGRIAKRLVDCGVFEADKMPNHVLVNDYAPGEGIAGHTDGPAYYPLVATLSLGSDAIMQFEPRVSSDVIGTPLHKSLLSTQSCEVLLQRRSVVIFKGDAYHNMLHSIPSRITE 588
            +  +EEE  L  + +    +W QL HRRLQ +GG     G+   EE+P WL +   ++  C +FE +K+PNHVL+N+Y PG+GI  H+DGP ++P+V T+S GS  ++ F  R+ S    T  H+  L       LL+RRS++I +GD YH+ LHSI  + T+
Sbjct:   27 ITPEEEEEILRCVNNAPLPKWTQLSHRRLQNWGGIPHPKGMI-AEEIPSWLMKYVNKISSCNIFEKEKLPNHVLINEYLPGQGIMAHSDGPLFHPIVTTISCGSHTLLDFYKRLDS----TQQHELNLEFS---FLLERRSLLILQGDLYHHYLHSIAEKDTD 181          
BLAST of mRNA_M-pyrifera_M_contig102007.435.1 vs. uniprot
Match: A0A482RD56_9ARCH (Fe2OG dioxygenase domain-containing protein (Fragment) n=1 Tax=archaeon TaxID=1906665 RepID=A0A482RD56_9ARCH)

HSP 1 Score: 130 bits (326), Expect = 3.740e-33
Identity = 70/140 (50.00%), Postives = 90/140 (64.29%), Query Frame = 1
Query:  157 RWQQLRHRRLQVYGGKVERDGVKDVEELPPWLGRIAKRLVDCGVFEADKMPNHVLVNDYAPGEGIAGHTDGPAYYPLVATLSLGSDAIMQFEPRVSSDVIGTPLHKSLLSTQSCEVLLQRRSVVIFKGDAYHNMLHSIPS 576
            RW QL+ RRLQ++GG V  DG+ DVE LPP+  RI   L+  GVF  +  PNH+L+N+Y PG+GI  HTDGPAY P VATLSLGS A+M+F  R  S        + L + +  E+LL   S+V+F  DAY  M H IP+
Sbjct:  143 RWVQLKQRRLQMWGGMVRADGLHDVEALPPFAERIVDALMRAGVFPTELRPNHLLINEYLPGQGIMPHTDGPAYTPRVATLSLGSAALMRFSERDRST-------RRLHARE--ELLLGAGSLVVFTDDAYTRMEHEIPA 273          
BLAST of mRNA_M-pyrifera_M_contig102007.435.1 vs. uniprot
Match: UPI0013047104 (alpha-ketoglutarate-dependent dioxygenase alkB homolog 6 n=1 Tax=Nomia melanderi TaxID=2448451 RepID=UPI0013047104)

HSP 1 Score: 127 bits (318), Expect = 5.320e-33
Identity = 67/164 (40.85%), Postives = 102/164 (62.20%), Query Frame = 1
Query:  103 VSKEEERWLMGMVDFEE-SRWQQLRHRRLQVYGGKVERDGVKDVEELPPWLGRIAKRLVDCGVFEADKMPNHVLVNDYAPGEGIAGHTDGPAYYPLVATLSLGSDAIMQF-EPRVSSDVIGTPLHKSLLSTQSCEVLLQRRSVVIFKGDAYHNMLHSIPSRITE 588
            +++EEE  +M  V+     +W QL HRRLQ +GG     G+   E++P WL +   +++ C +FE  K+PNHVL+N+Y PG+GI  H+DGP ++P+V T+S GS  ++ F E   +S++    L  SLL        L+RRS++I +GD YH+ LHSI  R T+
Sbjct:   23 ITEEEELEIMKYVNNAPLPKWNQLSHRRLQNWGGIPHPKGMI-AEDIPSWLQKYINKVISCNIFETSKLPNHVLINEYLPGQGIMAHSDGPLFHPIVTTISCGSHILLDFYEQPENSEIQQRNLEFSLL--------LERRSLLILQGDLYHHYLHSIAERNTD 177          
BLAST of mRNA_M-pyrifera_M_contig102007.435.1 vs. uniprot
Match: A0A6P8LLC3_BOMIM (alpha-ketoglutarate-dependent dioxygenase alkB homolog 6 n=6 Tax=Bombus TaxID=28641 RepID=A0A6P8LLC3_BOMIM)

HSP 1 Score: 125 bits (313), Expect = 3.290e-32
Identity = 63/144 (43.75%), Postives = 89/144 (61.81%), Query Frame = 1
Query:  157 RWQQLRHRRLQVYGGKVERDGVKDVEELPPWLGRIAKRLVDCGVFEADKMPNHVLVNDYAPGEGIAGHTDGPAYYPLVATLSLGSDAIMQFEPRVSSDVIGTPLHKSLLSTQSCEVLLQRRSVVIFKGDAYHNMLHSIPSRITE 588
            +W QL HRRLQ +GG     G+   EE+P WL +   ++  C +FE +K+PNHVL+N+Y  G+GI  H+DGP ++P+V T+S GS  ++ F  R+ S    T  H+  L       LL+RRS+ I +GD YHN LHSI  R T+
Sbjct:   46 KWTQLTHRRLQNWGGNPHPRGMI-AEEIPSWLQKYVNKVSSCDIFEKNKLPNHVLLNEYLSGQGIMAHSDGPLFHPIVTTISCGSHTLLDFYKRLDS----TEQHQPNLEFS---FLLERRSLFILQGDLYHNYLHSIAERDTD 181          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig102007.435.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A0G4I4J7_9ALVE3.360e-3942.37Fe2OG dioxygenase domain-containing protein n=1 Ta... [more]
A0A7S0PB53_CAFRO2.030e-3850.00Hypothetical protein n=1 Tax=Cafeteria roenbergens... [more]
A0A7S2G3H8_9STRA1.010e-3747.50Hypothetical protein n=1 Tax=Dictyocha speculum Ta... [more]
UPI001F4F4B1C2.000e-3740.36alpha-ketoglutarate-dependent dioxygenase alkB hom... [more]
UPI0014780CBA2.100e-3341.10alpha-ketoglutarate-dependent dioxygenase alkB hom... [more]
A0A7S2RZS8_9STRA2.360e-3337.77Hypothetical protein n=1 Tax=labyrinthulid quahog ... [more]
UPI00061542432.960e-3340.49alpha-ketoglutarate-dependent dioxygenase alkB hom... [more]
A0A482RD56_9ARCH3.740e-3350.00Fe2OG dioxygenase domain-containing protein (Fragm... [more]
UPI00130471045.320e-3340.85alpha-ketoglutarate-dependent dioxygenase alkB hom... [more]
A0A6P8LLC3_BOMIM3.290e-3243.75alpha-ketoglutarate-dependent dioxygenase alkB hom... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig102007contigM-pyrifera_M_contig102007:2..595 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score126.7
Seed ortholog evalue1.2e-26
Seed eggNOG ortholog115417.EPrPW00000024882
KEGG koko:K10768
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Alkylated DNA repair protein alkB. Source PGD
EggNOG OGs1MFSG@121069,KOG3200@1,KOG3200@2759
COG Functional cat.S
Best tax levelPythiales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000
Exons1
Model size594
Cds size594
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815520.8355696-CDS-M-pyrifera_M_contig102007:1..5951622815520.8355696-CDS-M-pyrifera_M_contig102007:1..595Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig102007 2..595 -
1692277447.8572156-CDS-M-pyrifera_M_contig102007:1..5951692277447.8572156-CDS-M-pyrifera_M_contig102007:1..595Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig102007 2..595 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig102007.435.1prot_M-pyrifera_M_contig102007.435.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig102007 2..595 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig102007.435.1

>prot_M-pyrifera_M_contig102007.435.1 ID=prot_M-pyrifera_M_contig102007.435.1|Name=mRNA_M-pyrifera_M_contig102007.435.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=198bp
KRPISPPPLSPLPSYGERNALDAPVGEVGCLCELVSKEEERWLMGMVDFE
ESRWQQLRHRRLQVYGGKVERDGVKDVEELPPWLGRIAKRLVDCGVFEAD
KMPNHVLVNDYAPGEGIAGHTDGPAYYPLVATLSLGSDAIMQFEPRVSSD
VIGTPLHKSLLSTQSCEVLLQRRSVVIFKGDAYHNMLHSIPSRITEKL
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mRNA from alignment at M-pyrifera_M_contig102007:2..595-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig102007.435.1 ID=mRNA_M-pyrifera_M_contig102007.435.1|Name=mRNA_M-pyrifera_M_contig102007.435.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=594bp|location=Sequence derived from alignment at M-pyrifera_M_contig102007:2..595- (Macrocystis pyrifera P11B4 male)
AAACGGCCCATTTCTCCTCCTCCATTATCACCTCTCCCATCTTATGGAGA AAGAAATGCTTTGGATGCTCCGGTTGGTGAGGTTGGATGTTTGTGTGAGT TGGTGAGTAAGGAAGAAGAGCGGTGGTTAATGGGAATGGTGGATTTTGAG GAGAGTAGGTGGCAGCAATTGAGACATCGTCGATTGCAGGTATATGGGGG AAAGGTGGAGAGGGATGGAGTGAAGGATGTGGAGGAATTACCTCCTTGGT TGGGTAGAATCGCAAAGCGATTAGTTGATTGTGGTGTATTCGAGGCAGAT AAGATGCCAAATCATGTGTTGGTGAATGATTATGCACCAGGGGAAGGAAT TGCAGGACATACAGATGGTCCGGCATACTACCCATTGGTTGCAACCCTTT CTCTTGGCAGTGATGCAATCATGCAATTTGAGCCTCGAGTATCAAGTGAT GTTATTGGAACACCTCTCCATAAATCCTTGCTCTCTACGCAATCTTGCGA AGTCCTCTTGCAAAGAAGGTCCGTTGTGATCTTCAAAGGAGATGCGTATC ACAACATGCTTCACTCCATCCCTTCAAGAATCACAGAAAAACTG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig102007:2..595-

>mRNA_M-pyrifera_M_contig102007.435.1 ID=mRNA_M-pyrifera_M_contig102007.435.1|Name=mRNA_M-pyrifera_M_contig102007.435.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1188bp|location=Sequence derived from alignment at M-pyrifera_M_contig102007:2..595- (Macrocystis pyrifera P11B4 male)
AAACGGCCCATTTCTCCTCCTCCATTATCACCTCTCCCATCTTATGGAGA
AAGAAATGCTTTGGATGCTCCGGTTGGTGAGGTTGGATGTTTGTGTGAGT
TGGTGAGTAAGGAAGAAGAGCGGTGGTTAATGGGAATGGTGGATTTTGAG
GAGAGTAGGTGGCAGCAATTGAGACATCGTCGATTGCAGGTATATGGGGG
AAAGGTGGAGAGGGATGGAGTGAAGGATGTGGAGGAATTACCTCCTTGGT
TGGGTAGAATCGCAAAGCGATTAGTTGATTGTGGTGTATTCGAGGCAGAT
AAGATGCCAAATCATGTGTTGGTGAATGATTATGCACCAGGGGAAGGAAT
TGCAGGACATACAGATGGTCCGGCATACTACCCATTGGTTGCAACCCTTT
CTCTTGGCAGTGATGCAATCATGCAATTTGAGCCTCGAGTATCAAGTGAT
GTTATTGGAACACCTCTCCATAAATCCTTGCTCTCTACGCAATCTTGCGA
AGTCCTCTTGCAAAGAAGGTCCGTTGTGATCTTCAAAGGAGATGCGTATC
ACAACATGCTTCACTCCATCCCTTCAAGAATCACAGAAAAACTGAAACGG
CCCATTTCTCCTCCTCCATTATCACCTCTCCCATCTTATGGAGAAAGAAA
TGCTTTGGATGCTCCGGTTGGTGAGGTTGGATGTTTGTGTGAGTTGGTGA
GTAAGGAAGAAGAGCGGTGGTTAATGGGAATGGTGGATTTTGAGGAGAGT
AGGTGGCAGCAATTGAGACATCGTCGATTGCAGGTATATGGGGGAAAGGT
GGAGAGGGATGGAGTGAAGGATGTGGAGGAATTACCTCCTTGGTTGGGTA
GAATCGCAAAGCGATTAGTTGATTGTGGTGTATTCGAGGCAGATAAGATG
CCAAATCATGTGTTGGTGAATGATTATGCACCAGGGGAAGGAATTGCAGG
ACATACAGATGGTCCGGCATACTACCCATTGGTTGCAACCCTTTCTCTTG
GCAGTGATGCAATCATGCAATTTGAGCCTCGAGTATCAAGTGATGTTATT
GGAACACCTCTCCATAAATCCTTGCTCTCTACGCAATCTTGCGAAGTCCT
CTTGCAAAGAAGGTCCGTTGTGATCTTCAAAGGAGATGCGTATCACAACA
TGCTTCACTCCATCCCTTCAAGAATCACAGAAAAACTG
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