mRNA_M-pyrifera_M_contig100845.186.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig100845.186.1
Unique NamemRNA_M-pyrifera_M_contig100845.186.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig100845.186.1 vs. uniprot
Match: A0A7C2J6D0_9BACT (NAD(P)-dependent oxidoreductase n=1 Tax=Bryobacteraceae bacterium TaxID=2212468 RepID=A0A7C2J6D0_9BACT)

HSP 1 Score: 150 bits (379), Expect = 2.110e-41
Identity = 81/158 (51.27%), Postives = 98/158 (62.03%), Query Frame = 1
Query:   22 DDSFRDVRVLVTGAAGFVGRWVARCLSASQAALTLAVRDAQAAKPIFARWGVEGAPVEADLARPGAGRELVEAYGPQVIFNLAGYGVDRSEKDRELGRRINTELVCELAAAVSRTSPGDWSGQRFVHVGSAFEYGWVGGDLREDGPVEPIGWYGESKL 495
            D  F  VRVLVTGAAGF+GRWVAR L+ + A L L V + ++A  +FAR+ V G  +E DL  P +   LV A  P V+FNL GYG++RSE+D  L  RIN +L  ELA A+       W G   VHVGS  EYG VGGDL ED P  P   YG +KL
Sbjct:   15 DRHFAGVRVLVTGAAGFIGRWVARRLTEAGADLILPVLERRSADAVFARYAVRGTILELDLGSPDSVEPLVRAARPLVVFNLVGYGINRSERDESLFWRINADLPAELARAMGEHRERGWPGASVVHVGSIAEYGPVGGDLAEDSPARPATLYGRTKL 172          
BLAST of mRNA_M-pyrifera_M_contig100845.186.1 vs. uniprot
Match: A0A7C2UDX5_9BACT (NAD(P)-dependent oxidoreductase n=1 Tax=Acidobacteria bacterium TaxID=1978231 RepID=A0A7C2UDX5_9BACT)

HSP 1 Score: 147 bits (370), Expect = 3.930e-40
Identity = 77/156 (49.36%), Postives = 97/156 (62.18%), Query Frame = 1
Query:   28 SFRDVRVLVTGAAGFVGRWVARCLSASQAALTLAVRDAQAAKPIFARWGVEGAPVEADLARPGAGRELVEAYGPQVIFNLAGYGVDRSEKDRELGRRINTELVCELAAAVSRTSPGDWSGQRFVHVGSAFEYGWVGGDLREDGPVEPIGWYGESKL 495
            ++R  RVLVTGA GF+GRW AR L+ + A L L+ RDA   + +   +G+ G   EADLA P A   L     P  IFNLAGYGVDR E+D  L   INT LV E+A  ++      W GQ+ +HVGSAFEYG V G+L E+ P +P   YGE+KL
Sbjct:   10 AYRGRRVLVTGATGFIGRWAARALTEAGADLCLSGRDAALLRAVCDVYGIRGEISEADLAEPEAFARLYTQARPAAIFNLAGYGVDREERDPALSAAINTRLVQEMAEVIASGGASGWLGQQLIHVGSAFEYGPVEGELTEESPTKPTSLYGETKL 165          
BLAST of mRNA_M-pyrifera_M_contig100845.186.1 vs. uniprot
Match: A0A2D5YVL8_9PLAN (Epimerase domain-containing protein n=1 Tax=Planctomycetaceae bacterium TaxID=2026779 RepID=A0A2D5YVL8_9PLAN)

HSP 1 Score: 145 bits (367), Expect = 1.230e-39
Identity = 80/152 (52.63%), Postives = 95/152 (62.50%), Query Frame = 1
Query:   40 VRVLVTGAAGFVGRWVARCLSASQAALTLAVRDAQAAKPIFARWGVEGAPVEADLARPGAGRELVEAYGPQVIFNLAGYGVDRSEKDRELGRRINTELVCELAAAVSRTSPGDWSGQRFVHVGSAFEYGWVGGDLREDGPVEPIGWYGESKL 495
            VR  V GA+GFVGRW AR L A  A + L VRDA A   +   +G++G  V AD+A   A R +++   P V+FNL GYGVDRSE+D    RRIN ELV  LA A +      W+GQR VHVGSA EYG   GDLREDGP  P   YG +KL
Sbjct:   22 VRAAVLGASGFVGRWGARRLCARGADVHLVVRDAGAMARVGRDYGIDGTVVTADMADGDAVRGMIDEIRPTVVFNLIGYGVDRSERDAATARRINAELVEVLAEATAGLGETSWTGQRLVHVGSALEYGEASGDLREDGPTAPTTDYGITKL 173          
BLAST of mRNA_M-pyrifera_M_contig100845.186.1 vs. uniprot
Match: A0A7W1GYV7_9BACT (NAD(P)-dependent oxidoreductase n=1 Tax=Gemmatimonadaceae bacterium TaxID=2026741 RepID=A0A7W1GYV7_9BACT)

HSP 1 Score: 139 bits (349), Expect = 5.310e-37
Identity = 71/156 (45.51%), Postives = 95/156 (60.90%), Query Frame = 1
Query:   28 SFRDVRVLVTGAAGFVGRWVARCLSASQAALTLAVRDAQAAKPIFARWGVEGAPVEADLARPGAGRELVEAYGPQVIFNLAGYGVDRSEKDRELGRRINTELVCELAAAVSRTSPGDWSGQRFVHVGSAFEYGWVGGDLREDGPVEPIGWYGESKL 495
            S+     +VTGA+GF+GRWVAR L A  A + L VR+A +A  +          ++ADL R  A RE++ +  P+++FNLAGYGVDR E+D  +   INTEL   +  A++  S   W GQR VHVGSA EYG +GGDL E     P   YG++KL
Sbjct:   15 SYGSATAVVTGASGFIGRWVARALLAHDAKVVLVVRNATSALNLGLDDDARCTVMQADLMRDNAAREVIASCRPEIVFNLAGYGVDRGERDEHVAHAINTELPVRIVEALAEQSDSTWRGQRLVHVGSALEYGEIGGDLSESCVPNPTTVYGQTKL 170          
BLAST of mRNA_M-pyrifera_M_contig100845.186.1 vs. uniprot
Match: A0A7Y3EET3_9BACT (NAD-dependent epimerase/dehydratase family protein (Fragment) n=1 Tax=Gemmatimonadetes bacterium TaxID=2026742 RepID=A0A7Y3EET3_9BACT)

HSP 1 Score: 124 bits (310), Expect = 4.050e-33
Identity = 72/145 (49.66%), Postives = 83/145 (57.24%), Query Frame = 1
Query:   49 LVTGAAGFVGRWVARCLSASQAALTLAVRDAQAAKPIFARWGVEGAPVEADLARPGAGRELVEAYGPQVIFNLAGYGVDRSEKDRELGRRINTELVCELAAAVSRTSPGDWSGQRFVHVGSAFEYGWVGGDLREDGPVEPIGWYG 483
            LV GA GFVGRWVAR LSA  A L LAVRD   A P+F+ + ++GA    DL +PG  RELV    P  +FNLAGYGVDRSE+D      IN +LV  +   +    P   S    VHVGSA EYG   GDL E     P   YG
Sbjct:   12 LVLGATGFVGRWVARRLSAVGADLHLAVRDPARAHPVFSAYAIDGAVHPIDLRQPGEVRELVARTRPDTVFNLAGYGVDRSERDESAAFAINRDLVAAVCEILLDVEPA--SRPALVHVGSALEYGQAAGDLNETTAPRPDTLYG 154          
BLAST of mRNA_M-pyrifera_M_contig100845.186.1 vs. uniprot
Match: A0A7Y2HPP2_9BACT (NAD(P)-dependent oxidoreductase n=1 Tax=Gemmatimonadetes bacterium TaxID=2026742 RepID=A0A7Y2HPP2_9BACT)

HSP 1 Score: 126 bits (317), Expect = 1.920e-32
Identity = 73/146 (50.00%), Postives = 84/146 (57.53%), Query Frame = 1
Query:   58 GAAGFVGRWVARCLSASQAALTLAVRDAQAAKPIFARWGVEGAPVEADLARPGAGRELVEAYGPQVIFNLAGYGVDRSEKDRELGRRINTELVCELAAAVSRTSPGDWSGQRFVHVGSAFEYGWVGGDLREDGPVEPIGWYGESKL 495
            GA GFVGRWVAR LSA  A L LAVRD   A P+F+ + ++GA    DL +PG  RELV    P  +FNLAGYGVDRSE+D      IN +LV  +   +    P   S    VHVGSA EYG   GDL E     P   YG SKL
Sbjct:    3 GATGFVGRWVARRLSAVGADLHLAVRDPARAHPVFSAYAIDGAVHPIDLRQPGEVRELVARTRPDTVFNLAGYGVDRSERDESAAFAINRDLVAAVCEILLDVEPA--SRPALVHVGSALEYGQAAGDLNETTAPRPDTLYGTSKL 146          
BLAST of mRNA_M-pyrifera_M_contig100845.186.1 vs. uniprot
Match: A0A3C0JK53_9GAMM (Epimerase domain-containing protein n=2 Tax=Gammaproteobacteria TaxID=1236 RepID=A0A3C0JK53_9GAMM)

HSP 1 Score: 124 bits (311), Expect = 2.210e-31
Identity = 69/157 (43.95%), Postives = 92/157 (58.60%), Query Frame = 1
Query:   28 SFRDVRVLVTGAAGFVGRWVARCLSASQAALTLAVRDAQAAKPIFARWGVEGAPVEADLARPGAGR-ELVEAYGPQVIFNLAGYGVDRSEKDRELGRRINTELVCELAAAVSRTSPGDWSGQRFVHVGSAFEYGWVGGDLREDGPVEPIGWYGESKL 495
            S++D  V++ GA+GF+GRW+AR LS   A + L VRD  AAK IF ++GV G   E +L         + +   P V+FNLAGYGV+ S++D+ L   IN +LV  +  A++     +W GQ  VHVGSA EYG    DL E     P   YGESKL
Sbjct:   14 SYKDKNVVILGASGFIGRWLARALSKIGANIFLFVRDKHAAKRIFDQFGVSGVVFELNLVDDAESLINIFKKISPDVVFNLAGYGVNPSQRDQNLAYTINADLVSIVCKAMATAKGDNWVGQDIVHVGSALEYGKDSSDLSESIACVPTTLYGESKL 170          
BLAST of mRNA_M-pyrifera_M_contig100845.186.1 vs. uniprot
Match: A0A1E4B037_9BACT (Epimerase domain-containing protein n=1 Tax=Gemmatimonadetes bacterium SCN 70-22 TaxID=1660163 RepID=A0A1E4B037_9BACT)

HSP 1 Score: 124 bits (311), Expect = 2.380e-31
Identity = 76/155 (49.03%), Postives = 84/155 (54.19%), Query Frame = 1
Query:   31 FRDVRVLVTGAAGFVGRWVARCLSASQAALTLAVRDAQAAKPIFARWGVEGAPVEADLARPGAGRELVEAYGPQVIFNLAGYGVDRSEKDRELGRRINTELVCELAAAVSRTSPGDWSGQRFVHVGSAFEYGWVGGDLREDGPVEPIGWYGESKL 495
            F   RVLV GA+GFVG WVAR L A  A L  AVR    A+ + ARW +    V  +LA   A    V A  P VIFNLAGYGVDR E+D   G  +N   V  L   V      DW G R VHVGSA EYG  GG+L E  P  P   YG SKL
Sbjct:    8 FLYTRVLVLGASGFVGHWVARALRAQGAHLICAVRTIAGAERL-ARWQLGTVVVRRNLADLDALASWVPALRPSVIFNLAGYGVDRGERDEAEGDLLNHRFVEVLGHVVRGMPRDDWEGVRLVHVGSALEYGTTGGELHESSPCAPTTAYGRSKL 161          
BLAST of mRNA_M-pyrifera_M_contig100845.186.1 vs. uniprot
Match: A0A7W1SDG1_9BACT (NAD-dependent epimerase/dehydratase n=1 Tax=Acidobacteria bacterium TaxID=1978231 RepID=A0A7W1SDG1_9BACT)

HSP 1 Score: 122 bits (307), Expect = 7.190e-31
Identity = 70/158 (44.30%), Postives = 89/158 (56.33%), Query Frame = 1
Query:   22 DDSFRDVRVLVTGAAGFVGRWVARCLSASQAALTLAVRDAQAAKPIFARWGVEGAPVEADLARPGAGRELVEAYGPQVIFNLAGYGVDRSEKDRELGRRINTELVCELAAAVSRTSPGDWSGQRFVHVGSAFEYGWVGGDLREDGPVEPIGWYGESKL 495
            D ++R V  LV G +GF+G W AR L AS AA+T+A RD   A         +   + ADL    A   L+E+  P ++FNLAGYGVD SE+D  L   +N  +V  L A +S      W+GQR VH GSA EYG V G L ED P+ P   YG +KL
Sbjct:    6 DHAYRGVPALVLGGSGFIGAWTARALHASGAAVTVAARDTGRAAVALRTIARDVCVLGADLVEATALDALLESARPSIVFNLAGYGVDASERDPVLMAALNARVVEGLCARLSGAMDDGWTGQRLVHAGSALEYGPVLGPLHEDMPLNPTTDYGRTKL 163          
BLAST of mRNA_M-pyrifera_M_contig100845.186.1 vs. uniprot
Match: A0A3E0PBZ7_9BACT (NAD(P)-dependent oxidoreductase n=1 Tax=Acidobacteria bacterium TaxID=1978231 RepID=A0A3E0PBZ7_9BACT)

HSP 1 Score: 122 bits (306), Expect = 1.400e-30
Identity = 68/165 (41.21%), Postives = 95/165 (57.58%), Query Frame = 1
Query:   10 PLARDDSFRD---VRVLVTGAAGFVGRWVARCLSASQAALTLAVRDAQAAKPIFARWGVEGAPVEADLARPGAGRELVEAYGPQVIFNLAGYGVDRSEKDRELGRRINTELVCELAAAVSRTSPGDWSGQRFVHVGSAFEYGWVGGDLREDGPVEPIGWYGESKL 495
            P  ++D  R+    R LV GA GF+G WV+R LS + A +T A+R+  +A  +   +GV      +DL   G+ + L+ +  P ++FNLAGYG+DR+E D E   +IN+ LV ++  AV     G W+  R VH GSA EYG V G+L ED    P   YG SKL
Sbjct:   15 PAVKNDPLREYEGARALVLGAGGFIGLWVSRLLSEAGAEVTTALRNGASAG-LLETFGVRSEIRVSDLMTAGSLKHLISSARPSIVFNLAGYGIDRTETDEETAFKINSGLVNQVCEAVDSGPAGGWNKLRVVHAGSAMEYGAVPGNLEEDSDTRPTTVYGRSKL 178          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig100845.186.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7C2J6D0_9BACT2.110e-4151.27NAD(P)-dependent oxidoreductase n=1 Tax=Bryobacter... [more]
A0A7C2UDX5_9BACT3.930e-4049.36NAD(P)-dependent oxidoreductase n=1 Tax=Acidobacte... [more]
A0A2D5YVL8_9PLAN1.230e-3952.63Epimerase domain-containing protein n=1 Tax=Planct... [more]
A0A7W1GYV7_9BACT5.310e-3745.51NAD(P)-dependent oxidoreductase n=1 Tax=Gemmatimon... [more]
A0A7Y3EET3_9BACT4.050e-3349.66NAD-dependent epimerase/dehydratase family protein... [more]
A0A7Y2HPP2_9BACT1.920e-3250.00NAD(P)-dependent oxidoreductase n=1 Tax=Gemmatimon... [more]
A0A3C0JK53_9GAMM2.210e-3143.95Epimerase domain-containing protein n=2 Tax=Gammap... [more]
A0A1E4B037_9BACT2.380e-3149.03Epimerase domain-containing protein n=1 Tax=Gemmat... [more]
A0A7W1SDG1_9BACT7.190e-3144.30NAD-dependent epimerase/dehydratase n=1 Tax=Acidob... [more]
A0A3E0PBZ7_9BACT1.400e-3041.21NAD(P)-dependent oxidoreductase n=1 Tax=Acidobacte... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig100845contigM-pyrifera_M_contig100845:176..670 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score60.8
Seed ortholog evalue6.7e-07
Seed eggNOG ortholog666686.B1NLA3E_08965
KEGG rclassRC00289
KEGG koko:K01784,ko:K20534
KEGG TC4.D.2.1.9
KEGG ReactionR00291,R02984
KEGG Pathwayko00052,ko00520,ko01100,map00052,map00520,map01100
KEGG ModuleM00361,M00362,M00632
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Male sterility protein
EggNOG OGs1VP6I@1239,1ZJS0@1386,4HAN7@91061,COG0451@1,COG0451@2
EC5.1.3.2
COG Functional cat.GM
CAZyGT2
Best tax levelBacillus
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko01005,ko02000
Exons1
Model size495
Cds size495
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815505.7856898-CDS-M-pyrifera_M_contig100845:175..6701622815505.7856898-CDS-M-pyrifera_M_contig100845:175..670Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig100845 176..670 +
1692277441.8107731-CDS-M-pyrifera_M_contig100845:175..6701692277441.8107731-CDS-M-pyrifera_M_contig100845:175..670Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig100845 176..670 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig100845.186.1prot_M-pyrifera_M_contig100845.186.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig100845 176..670 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig100845.186.1

>prot_M-pyrifera_M_contig100845.186.1 ID=prot_M-pyrifera_M_contig100845.186.1|Name=mRNA_M-pyrifera_M_contig100845.186.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=165bp
MKQPLARDDSFRDVRVLVTGAAGFVGRWVARCLSASQAALTLAVRDAQAA
KPIFARWGVEGAPVEADLARPGAGRELVEAYGPQVIFNLAGYGVDRSEKD
RELGRRINTELVCELAAAVSRTSPGDWSGQRFVHVGSAFEYGWVGGDLRE
DGPVEPIGWYGESKL
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mRNA from alignment at M-pyrifera_M_contig100845:176..670+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig100845.186.1 ID=mRNA_M-pyrifera_M_contig100845.186.1|Name=mRNA_M-pyrifera_M_contig100845.186.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=495bp|location=Sequence derived from alignment at M-pyrifera_M_contig100845:176..670+ (Macrocystis pyrifera P11B4 male)
ATGAAACAACCTCTGGCAAGAGACGACTCATTTCGAGATGTGCGGGTTCT TGTGACCGGCGCGGCCGGGTTTGTTGGGCGTTGGGTTGCTCGTTGCCTAT CAGCATCTCAGGCGGCGCTAACGCTAGCCGTGCGAGATGCTCAGGCGGCG AAACCGATCTTCGCAAGGTGGGGTGTAGAGGGTGCGCCGGTTGAAGCCGA TCTCGCTCGTCCTGGAGCCGGGCGGGAACTAGTCGAAGCGTATGGGCCTC AGGTCATCTTCAATCTGGCGGGCTATGGTGTGGACCGCTCGGAAAAAGAC CGTGAGCTAGGGAGACGCATCAATACCGAATTGGTCTGCGAACTCGCAGC CGCAGTGAGTCGCACAAGCCCTGGCGATTGGTCAGGGCAGCGGTTCGTAC ACGTTGGATCGGCGTTCGAGTATGGTTGGGTTGGCGGCGATCTGCGCGAA GACGGCCCGGTTGAGCCAATTGGGTGGTACGGTGAGAGCAAACTC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig100845:176..670+

>mRNA_M-pyrifera_M_contig100845.186.1 ID=mRNA_M-pyrifera_M_contig100845.186.1|Name=mRNA_M-pyrifera_M_contig100845.186.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=990bp|location=Sequence derived from alignment at M-pyrifera_M_contig100845:176..670+ (Macrocystis pyrifera P11B4 male)
ATGAAACAACCTCTGGCAAGAGACGACTCATTTCGAGATGTGCGGGTTCT
TGTGACCGGCGCGGCCGGGTTTGTTGGGCGTTGGGTTGCTCGTTGCCTAT
CAGCATCTCAGGCGGCGCTAACGCTAGCCGTGCGAGATGCTCAGGCGGCG
AAACCGATCTTCGCAAGGTGGGGTGTAGAGGGTGCGCCGGTTGAAGCCGA
TCTCGCTCGTCCTGGAGCCGGGCGGGAACTAGTCGAAGCGTATGGGCCTC
AGGTCATCTTCAATCTGGCGGGCTATGGTGTGGACCGCTCGGAAAAAGAC
CGTGAGCTAGGGAGACGCATCAATACCGAATTGGTCTGCGAACTCGCAGC
CGCAGTGAGTCGCACAAGCCCTGGCGATTGGTCAGGGCAGCGGTTCGTAC
ACGTTGGATCGGCGTTCGAGTATGGTTGGGTTGGCGGCGATCTGCGCGAA
GACGGCCCGGTTGAGCCAATTGGGTGGTACGGTGAGAGCAAACTCATGAA
ACAACCTCTGGCAAGAGACGACTCATTTCGAGATGTGCGGGTTCTTGTGA
CCGGCGCGGCCGGGTTTGTTGGGCGTTGGGTTGCTCGTTGCCTATCAGCA
TCTCAGGCGGCGCTAACGCTAGCCGTGCGAGATGCTCAGGCGGCGAAACC
GATCTTCGCAAGGTGGGGTGTAGAGGGTGCGCCGGTTGAAGCCGATCTCG
CTCGTCCTGGAGCCGGGCGGGAACTAGTCGAAGCGTATGGGCCTCAGGTC
ATCTTCAATCTGGCGGGCTATGGTGTGGACCGCTCGGAAAAAGACCGTGA
GCTAGGGAGACGCATCAATACCGAATTGGTCTGCGAACTCGCAGCCGCAG
TGAGTCGCACAAGCCCTGGCGATTGGTCAGGGCAGCGGTTCGTACACGTT
GGATCGGCGTTCGAGTATGGTTGGGTTGGCGGCGATCTGCGCGAAGACGG
CCCGGTTGAGCCAATTGGGTGGTACGGTGAGAGCAAACTC
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