mRNA_M-pyrifera_M_contig100671.155.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig100671.155.1
Unique NamemRNA_M-pyrifera_M_contig100671.155.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig100671.155.1 vs. uniprot
Match: A0A7X8ULJ6_9BACT (Peptide chain release factor 3 n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A7X8ULJ6_9BACT)

HSP 1 Score: 172 bits (435), Expect = 2.720e-48
Identity = 84/143 (58.74%), Postives = 99/143 (69.23%), Query Frame = 1 
Query:    1 DEIPFFAPECFTYIENVHPVQSKRFRTGLEQLLQEGVMQQYRLPDAVRNVPLLGAAGPLQFEIVQHRLESEYGAETRLQHAPWTETRWVAPSVEIVGKPLPPGVRLAYDGNERPVLLFESTWVMNYFMEKNLDISLAKLPFQD 429
            DEIP FAPECF +IEN  P   KRFR GL+QLLQEGV+Q Y L DA R VPLLGA GPLQFEIVQ+RL+SEYGA++RL+ A WT  RW+ PS       +P   RLA DG  RPVLLF   W + YF  +N  + ++ LPF D
Sbjct:  392 DEIPRFAPECFAFIENPTPANYKRFREGLDQLLQEGVVQCYDLADASRRVPLLGAVGPLQFEIVQYRLQSEYGAQSRLEPAQWTVARWLDPSANADSLVMPTSARLALDGQRRPVLLFPDEWNLRYFENRNPGVGISDLPFPD 534
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BLAST of mRNA_M-pyrifera_M_contig100671.155.1 vs. uniprot
Match: A0A7C1EAR3_9BACT (Peptide chain release factor 3 n=1 Tax=Phycisphaerales bacterium TaxID=2052180 RepID=A0A7C1EAR3_9BACT)

HSP 1 Score: 165 bits (417), Expect = 1.180e-45
Identity = 78/142 (54.93%), Postives = 99/142 (69.72%), Query Frame = 1 
Query:    4 EIPFFAPECFTYIENVHPVQSKRFRTGLEQLLQEGVMQQYRLPDAVRNVPLLGAAGPLQFEIVQHRLESEYGAETRLQHAPWTETRWVAPSVEIVGKPLPPGVRLAYDGNERPVLLFESTWVMNYFMEKNLDISLAKLPFQD 429
            EIP FAPECF Y+EN     SKRF +GL QLLQEGV+Q Y LP A+R VPLLGA GPLQF+IVQ+RLESEYG  +R+  APW+ +RW++P+ ++    LP G     DG  RPVLL    W + YF  +N D+ ++ +PFQD
Sbjct:  395 EIPRFAPECFAYLENPVSGNSKRFSSGLTQLLQEGVIQCYELPSAMRRVPLLGAVGPLQFDIVQYRLESEYGVTSRIVSAPWSLSRWLSPTADVTTLKLPQGACPVLDGQGRPVLLLSDDWSLRYFQMQNPDVQVSDIPFQD 536
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BLAST of mRNA_M-pyrifera_M_contig100671.155.1 vs. uniprot
Match: A0A7X7SB55_9BACT (Peptide chain release factor 3 n=1 Tax=Lentisphaerae bacterium TaxID=1932692 RepID=A0A7X7SB55_9BACT)

HSP 1 Score: 162 bits (409), Expect = 1.500e-44
Identity = 76/141 (53.90%), Postives = 99/141 (70.21%), Query Frame = 1 
Query:    1 DEIPFFAPECFTYIENVHPVQSKRFRTGLEQLLQEGVMQQYRLPDAVRNVPLLGAAGPLQFEIVQHRLESEYGAETRLQHAPWTETRWVAPSVE-IVGKPLPPGVRLAYDGNERPVLLFESTWVMNYFMEKNLDISLAKLP 420
            D +P FAPECF ++ +  P Q KRFR GL+ LLQEG +Q +++PD+    PLLGA GPLQFE++Q+RLE+EYGA TRL+ APW   RWV P+ E +    LP G RLA D  +RPV LF + W +N+FMEK+  + L KLP
Sbjct:  385 DPLPQFAPECFAWLHHSSPAQFKRFRAGLDHLLQEGAVQAFQIPDSATRAPLLGAVGPLQFEVLQYRLETEYGATTRLEPAPWKLLRWVDPAGEPVTSDALPSGARLAIDALQRPVALFSADWELNFFMEKHPQVLLRKLP 525
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BLAST of mRNA_M-pyrifera_M_contig100671.155.1 vs. uniprot
Match: A0A6N6PFQ2_9BACT (Peptide chain release factor 3 n=1 Tax=Verrucomicrobia bacterium TaxID=2026799 RepID=A0A6N6PFQ2_9BACT)

HSP 1 Score: 160 bits (405), Expect = 5.610e-44
Identity = 76/140 (54.29%), Postives = 98/140 (70.00%), Query Frame = 1 
Query:    1 DEIPFFAPECFTYIENVHPVQSKRFRTGLEQLLQEGVMQQYRLPDAVRNVPLLGAAGPLQFEIVQHRLESEYGAETRLQHAPWTETRWVAPSVEIVGKPLPPGVRLAYDGNERPVLLFESTWVMNYFMEKNLDISLAKLP 420
            DEIP FAPECF Y++N +P + K+FR G++QLLQEGV+Q + L DAV+ VPLL A GPLQFE+VQ+RLE+EYGA +RL++A W   +W+ P  E  G   P G RLAYD   +P +LF S W   YF E+N  + L  LP
Sbjct:  393 DEIPRFAPECFAYLQNPNPGKFKQFRQGVDQLLQEGVVQVFELRDAVQKVPLLAAVGPLQFEVVQYRLEAEYGAPSRLENASWACFKWLPPGTETAGLKFPTGCRLAYDSAGQPGVLFPSAWTQKYFTEQNGGLPLLDLP 532
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BLAST of mRNA_M-pyrifera_M_contig100671.155.1 vs. uniprot
Match: A0A7X7F7E3_9BACT (Peptide chain release factor 3 n=1 Tax=Lentisphaerae bacterium TaxID=1932692 RepID=A0A7X7F7E3_9BACT)

HSP 1 Score: 159 bits (401), Expect = 2.690e-43
Identity = 81/140 (57.86%), Postives = 92/140 (65.71%), Query Frame = 1 
Query:    1 DEIPFFAPECFTYIENVHPVQSKRFRTGLEQLLQEGVMQQYRLPDAVRNVPLLGAAGPLQFEIVQHRLESEYGAETRLQHAPWTETRWVAPSVEIVGKPLPPGVRLAYDGNERPVLLFESTWVMNYFMEKNLDISLAKLP 420
            DEIP FAPECF  IEN  P   KRFR GL+QLLQEGV+Q+Y LPD  R V LLGA GPLQFEIV+ RLESEY A +R+Q  PWT  RW  P     G  LP G RLA D   R V+LFE+ W + +F E + DI L   P
Sbjct:  393 DEIPRFAPECFAIIENPTPSAFKRFRKGLDQLLQEGVIQRYELPDLARKVTLLGAVGPLQFEIVKFRLESEYDAPSRIQAVPWTLVRWFDPDTRTDGISLPSGARLAEDDQGRTVILFENDWGLRFFKEDHPDIPLGDRP 532
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BLAST of mRNA_M-pyrifera_M_contig100671.155.1 vs. uniprot
Match: A0A7V3ACS4_9BACT (Peptide chain release factor 3 n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A7V3ACS4_9BACT)

HSP 1 Score: 155 bits (393), Expect = 2.930e-42
Identity = 72/142 (50.70%), Postives = 99/142 (69.72%), Query Frame = 1 
Query:    1 DEIPFFAPECFTYIENVHPVQSKRFRTGLEQLLQEGVMQQYRLPDAVRNVPLLGAAGPLQFEIVQHRLESEYGAETRLQHAPWTETRWVAPSVEIVGKPLPPGVRLAYDGNERPVLLFESTWVMNYFMEKNLDISLAKLPFQ 426
            DEI  FAPECF Y+E+  P   +RFRTGL+QLLQEGV+QQ+ +P   R VPLLGA GPLQ ++VQ+RLESEYGA +RL+  PW+ +RW+ P+       +  G  LA D   RPV+LF+  W + YF  +N D+ L+++P++
Sbjct:  390 DEISRFAPECFVYLESPVPAHYRRFRTGLDQLLQEGVVQQFTIPSDERGVPLLGAVGPLQIDVVQYRLESEYGAASRLEATPWSRSRWLDPATPKEKLNIASGACLAADSAGRPVVLFKDDWYLQYFARQNKDVGLSEVPYR 531
[Close]
BLAST of mRNA_M-pyrifera_M_contig100671.155.1 vs. uniprot
Match: A0A1V6JD01_9BACT (Peptide chain release factor 3 n=1 Tax=Verrucomicrobia bacterium ADurb.Bin018 TaxID=1852925 RepID=A0A1V6JD01_9BACT)

HSP 1 Score: 150 bits (380), Expect = 3.810e-42
Identity = 74/141 (52.48%), Postives = 94/141 (66.67%), Query Frame = 1 
Query:    1 DEIPFFAPECFTYIENVHPVQSKRFRTGLEQLLQEGVMQQYRLPDAVRNVPLLGAAGPLQFEIVQHRLESEYGAETRLQHAPWTETRWVAPSVEIVGKP-LPPGVRLAYDGNERPVLLFESTWVMNYFMEKNLDISLAKLP 420
            D +P FA ECF ++ +  P Q KRFR GL+ LLQEG +Q + LPD+    PLLGA GPLQFE++Q+RLE+EYGA TR + APWT  RWV P+ E V    LP   RLA+D   RPV LF + W + +F EKN  + L +LP
Sbjct:  170 DPLPQFAAECFAWLHHASPAQFKRFRAGLDHLLQEGAVQTFTLPDSGSRAPLLGAVGPLQFEVLQYRLENEYGAVTRREAAPWTILRWVDPAGEPVSPTMLPSSCRLAFDTANRPVALFSADWELKFFQEKNPRVILQRLP 310
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BLAST of mRNA_M-pyrifera_M_contig100671.155.1 vs. uniprot
Match: A0A3C1C2T6_9BACT (Peptide chain release factor 3 n=1 Tax=Verrucomicrobia bacterium TaxID=2026799 RepID=A0A3C1C2T6_9BACT)

HSP 1 Score: 155 bits (391), Expect = 5.480e-42
Identity = 74/141 (52.48%), Postives = 96/141 (68.09%), Query Frame = 1 
Query:    1 DEIPFFAPECFTYIENVHPVQSKRFRTGLEQLLQEGVMQQYRLPDAVRNVPLLGAAGPLQFEIVQHRLESEYGAETRLQHAPWTETRWVAPS-VEIVGKPLPPGVRLAYDGNERPVLLFESTWVMNYFMEKNLDISLAKLP 420
            D +P FA ECF Y+ +  P Q KRFR GL+ LLQEG +Q + +PD+    PLLGA GPLQFE++Q+RLESEYGA TRL+ APW+  RW+ P+   I    LP   RLA D ++RPV LF + W + +F+EKN  + L KLP
Sbjct:  385 DPLPQFAAECFAYLHHSSPAQFKRFRAGLDHLLQEGAVQTFTIPDSGSRAPLLGAVGPLQFEVLQYRLESEYGATTRLEPAPWSILRWIDPAGAPITPDMLPSSCRLALDAHDRPVALFSAAWELKFFVEKNPKVVLHKLP 525
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BLAST of mRNA_M-pyrifera_M_contig100671.155.1 vs. uniprot
Match: A0A832H881_9BACT (Peptide chain release factor 3 n=1 Tax=Verrucomicrobiales bacterium TaxID=2026801 RepID=A0A832H881_9BACT)

HSP 1 Score: 153 bits (387), Expect = 2.100e-41
Identity = 75/143 (52.45%), Postives = 96/143 (67.13%), Query Frame = 1 
Query:    1 DEIPFFAPECFTYIENVHPVQSKRFRTGLEQLLQEGVMQQYRLPDAVRNVPLLGAAGPLQFEIVQHRLESEYGAETRLQHAPWTETRWV--APSVEIVGKPLPPGVRLAYDGNERPVLLFESTWVMNYFMEKNLDISLAKLPF 423
            DEIP FAPECF Y+ N    QSKRFR GLEQLL EGV+Q + LP AV+ + LLGA GPLQFE+VQ+RLESEY A++RL+ APW   RW+      + +   LP G  +A D  ++PV LF   W + +F+E+N    L+ LPF
Sbjct:  385 DEIPRFAPECFAYLHNSSTAQSKRFREGLEQLLAEGVVQSFSLPHAVQRIALLGAVGPLQFEVVQYRLESEYAAQSRLEPAPWRVARWLHHPAGADPMACNLPTGTTIALDAQDQPVALFPEEWPVKFFLERNEGWQLSPLPF 527
[Close]
BLAST of mRNA_M-pyrifera_M_contig100671.155.1 vs. uniprot
Match: A0A832K6C5_9BACT (Peptide chain release factor 3 n=1 Tax=Verrucomicrobia subdivision 3 bacterium TaxID=2052186 RepID=A0A832K6C5_9BACT)

HSP 1 Score: 153 bits (386), Expect = 3.560e-41
Identity = 75/143 (52.45%), Postives = 98/143 (68.53%), Query Frame = 1 
Query:    4 EIPFFAPECFTYIENVHPVQSKRFRTGLEQLLQEGVMQQYRLPDAVRNVPLLGAAGPLQFEIVQHRLESEYGAETRLQHAPWTETRWVAPSV--EIVGKPLPPGVRLAYDGNERPVLLFESTWVMNYFMEKNLDISLAKLPFQ 426
            EIP F PECF ++++    Q KRFR GL+QLLQEGV+Q ++LPDA + VPLLGA GPLQFE+VQ+R+++EYGAE+RL+  PW   RW +P+    +    LP G RLAYD    PV+LF   W   +F E+N  I L+ LP Q
Sbjct:  387 EIPRFTPECFAWLQSPSTAQFKRFREGLDQLLQEGVVQAFQLPDAAQRVPLLGAVGPLQFEVVQYRMQTEYGAESRLEPGPWKILRWASPANGDRLDPNQLPTGARLAYDVAGHPVVLFPEQWSCEFFAERNPGIRLSALPPQ 529
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig100671.155.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7X8ULJ6_9BACT2.720e-4858.74Peptide chain release factor 3 n=1 Tax=Planctomyce... [more]
A0A7C1EAR3_9BACT1.180e-4554.93Peptide chain release factor 3 n=1 Tax=Phycisphaer... [more]
A0A7X7SB55_9BACT1.500e-4453.90Peptide chain release factor 3 n=1 Tax=Lentisphaer... [more]
A0A6N6PFQ2_9BACT5.610e-4454.29Peptide chain release factor 3 n=1 Tax=Verrucomicr... [more]
A0A7X7F7E3_9BACT2.690e-4357.86Peptide chain release factor 3 n=1 Tax=Lentisphaer... [more]
A0A7V3ACS4_9BACT2.930e-4250.70Peptide chain release factor 3 n=1 Tax=Planctomyce... [more]
A0A1V6JD01_9BACT3.810e-4252.48Peptide chain release factor 3 n=1 Tax=Verrucomicr... [more]
A0A3C1C2T6_9BACT5.480e-4252.48Peptide chain release factor 3 n=1 Tax=Verrucomicr... [more]
A0A832H881_9BACT2.100e-4152.45Peptide chain release factor 3 n=1 Tax=Verrucomicr... [more]
A0A832K6C5_9BACT3.560e-4152.45Peptide chain release factor 3 n=1 Tax=Verrucomicr... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig100671contigM-pyrifera_M_contig100671:246..677 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score150.2
Seed ortholog evalue7.3e-34
Seed eggNOG ortholog497964.CfE428DRAFT_4597
Preferred nameprfC
KEGG koko:K02837,ko:K07133
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003676,GO:0003723,GO:0003747,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0006412,GO:0006415,GO:0006518,GO:0006807,GO:0008079,GO:0008135,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009987,GO:0010467,GO:0016043,GO:0016150,GO:0019538,GO:0022411,GO:0032984,GO:0034641,GO:0034645,GO:0043043,GO:0043170,GO:0043603,GO:0043604,GO:0043624,GO:0043933,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044271,GO:0044424,GO:0044444,GO:0044464,GO:0071704,GO:0071840,GO:0097159,GO:1901363,GO:1901564,GO:1901566,GO:1901576
EggNOG free text desc.Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-2 is significantly reduced by GTP and GDP, but not by GMP
EggNOG OGs46SF9@74201,COG4108@1,COG4108@2
COG Functional cat.J
Best tax levelVerrucomicrobia
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03012
Exons1
Model size432
Cds size432
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815504.2080534-CDS-M-pyrifera_M_contig100671:245..6771622815504.2080534-CDS-M-pyrifera_M_contig100671:245..677Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig100671 246..677 -
1692277441.0543835-CDS-M-pyrifera_M_contig100671:245..6771692277441.0543835-CDS-M-pyrifera_M_contig100671:245..677Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig100671 246..677 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig100671.155.1prot_M-pyrifera_M_contig100671.155.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig100671 246..677 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig100671.155.1

>prot_M-pyrifera_M_contig100671.155.1 ID=prot_M-pyrifera_M_contig100671.155.1|Name=mRNA_M-pyrifera_M_contig100671.155.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=144bp
DEIPFFAPECFTYIENVHPVQSKRFRTGLEQLLQEGVMQQYRLPDAVRNV
PLLGAAGPLQFEIVQHRLESEYGAETRLQHAPWTETRWVAPSVEIVGKPL
PPGVRLAYDGNERPVLLFESTWVMNYFMEKNLDISLAKLPFQD*
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mRNA from alignment at M-pyrifera_M_contig100671:246..677-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig100671.155.1 ID=mRNA_M-pyrifera_M_contig100671.155.1|Name=mRNA_M-pyrifera_M_contig100671.155.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=432bp|location=Sequence derived from alignment at M-pyrifera_M_contig100671:246..677- (Macrocystis pyrifera P11B4 male)
GACGAGATTCCCTTCTTCGCTCCCGAGTGTTTTACCTATATCGAAAATGT
ACACCCGGTGCAGTCAAAGCGCTTTCGTACCGGTCTCGAGCAACTCTTGC
AGGAAGGCGTCATGCAGCAATACCGTTTGCCTGATGCCGTGCGAAACGTA
CCCCTGCTTGGTGCGGCCGGTCCGCTCCAGTTCGAAATCGTTCAGCACCG
ACTGGAGAGCGAGTACGGTGCCGAAACCCGCCTCCAGCACGCGCCCTGGA
CCGAAACCCGATGGGTCGCGCCGAGTGTAGAGATCGTGGGTAAGCCCCTG
CCGCCTGGCGTTCGCCTGGCCTATGATGGGAACGAGCGTCCCGTGTTGCT
TTTTGAAAGCACCTGGGTAATGAATTATTTCATGGAAAAGAACCTGGATA
TTAGCCTGGCCAAGTTGCCCTTTCAGGATTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig100671:246..677-

>mRNA_M-pyrifera_M_contig100671.155.1 ID=mRNA_M-pyrifera_M_contig100671.155.1|Name=mRNA_M-pyrifera_M_contig100671.155.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=864bp|location=Sequence derived from alignment at M-pyrifera_M_contig100671:246..677- (Macrocystis pyrifera P11B4 male)
GACGAGATTCCCTTCTTCGCTCCCGAGTGTTTTACCTATATCGAAAATGT
ACACCCGGTGCAGTCAAAGCGCTTTCGTACCGGTCTCGAGCAACTCTTGC
AGGAAGGCGTCATGCAGCAATACCGTTTGCCTGATGCCGTGCGAAACGTA
CCCCTGCTTGGTGCGGCCGGTCCGCTCCAGTTCGAAATCGTTCAGCACCG
ACTGGAGAGCGAGTACGGTGCCGAAACCCGCCTCCAGCACGCGCCCTGGA
CCGAAACCCGATGGGTCGCGCCGAGTGTAGAGATCGTGGGTAAGCCCCTG
CCGCCTGGCGTTCGCCTGGCCTATGATGGGAACGAGCGTCCCGTGTTGCT
TTTTGAAAGCACCTGGGTAATGAATTATTTCATGGAAAAGAACCTGGATA
TTAGCCTGGCCAAGTTGCCCTTTCAGGATTGAGACGAGATTCCCTTCTTC
GCTCCCGAGTGTTTTACCTATATCGAAAATGTACACCCGGTGCAGTCAAA
GCGCTTTCGTACCGGTCTCGAGCAACTCTTGCAGGAAGGCGTCATGCAGC
AATACCGTTTGCCTGATGCCGTGCGAAACGTACCCCTGCTTGGTGCGGCC
GGTCCGCTCCAGTTCGAAATCGTTCAGCACCGACTGGAGAGCGAGTACGG
TGCCGAAACCCGCCTCCAGCACGCGCCCTGGACCGAAACCCGATGGGTCG
CGCCGAGTGTAGAGATCGTGGGTAAGCCCCTGCCGCCTGGCGTTCGCCTG
GCCTATGATGGGAACGAGCGTCCCGTGTTGCTTTTTGAAAGCACCTGGGT
AATGAATTATTTCATGGAAAAGAACCTGGATATTAGCCTGGCCAAGTTGC
CCTTTCAGGATTGA
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