mRNA_L-elsbetiae_contig1520.3715.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig1520.3715.1
Unique NamemRNA_L-elsbetiae_contig1520.3715.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
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BLAST of mRNA_L-elsbetiae_contig1520.3715.1 vs. uniprot
Match: D8LEZ1_ECTSI (Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LEZ1_ECTSI)

HSP 1 Score: 159 bits (401), Expect = 7.060e-44
Identity = 81/113 (71.68%), Postives = 87/113 (76.99%), Query Frame = 1 
Query:    4 VLRETRGFDVAKGETTHLRRKEGSVDYRFCPEPDLPPLFVADEHVSALKAAMPELPEATMARFKRDYGLPEHLSRLIVCAAGGASGYFEDLVNGR-------TGEGREGLRRD 321
            + RETRGFDV+KGET HLRRKEGSVDYRF PEPDLPPL V  EHV AL+A +PELPEATMAR   +YGL EHLSRLIVCAAGGAS Y+EDLVNG         GEG    RRD
Sbjct:  292 IERETRGFDVSKGETIHLRRKEGSVDYRFFPEPDLPPLVVTHEHVEALRATLPELPEATMARLSAEYGLAEHLSRLIVCAAGGASDYYEDLVNGTGRERGHAAGEGSGPYRRD 404
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BLAST of mRNA_L-elsbetiae_contig1520.3715.1 vs. uniprot
Match: W7TQ61_9STRA (Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial n=3 Tax=Monodopsidaceae TaxID=425072 RepID=W7TQ61_9STRA)

HSP 1 Score: 100 bits (248), Expect = 2.260e-22
Identity = 51/102 (50.00%), Postives = 69/102 (67.65%), Query Frame = 1 
Query:    4 VLRETRGFDVAKGETTHLRRKEGSVDYRFCPEPDLPPLFVADEHVSALKAAMPELPEATMARFKRDYGLPEHLSRLIVCAAGGASGYFEDLVNGRTGEGREG 309
            V RETR F+ A GETT +R KEG+VDYRF PEPDLPPL +  + V A++  +PELP+AT +R   ++GL    + +++     A G+FED+V G  G GREG
Sbjct:  263 VERETRAFNAATGETTRMRGKEGAVDYRFFPEPDLPPLVLDMDWVEAVRRGLPELPQATRSRLVEEHGLTPAQAEVLIGEGEAAVGFFEDVVRG--GGGREG 362
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BLAST of mRNA_L-elsbetiae_contig1520.3715.1 vs. uniprot
Match: UPI001999D0CC (Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB n=1 Tax=Rhizobacter sp. TaxID=1909292 RepID=UPI001999D0CC)

HSP 1 Score: 95.1 bits (235), Expect = 1.140e-20
Identity = 43/92 (46.74%), Postives = 64/92 (69.57%), Query Frame = 1 
Query:    4 VLRETRGFDVAKGETTHLRRKEGSVDYRFCPEPDLPPLFVADEHVSALKAAMPELPEATMARFKRDYGLPEHLSRLIVCAAGGASGYFEDLV 279
            +L++TR FD  KG T  LR KE + DYR+ PEPDLPP ++AD+H++A+KAAMP LP AT  +FK  YGL ++    ++CA    + ++ +L+
Sbjct:  247 ILQQTRSFDADKGTTFALRSKEEADDYRYFPEPDLPPFYIADKHIAAIKAAMPTLPAATEQQFKERYGLGDY-DAAVICADKDDADFYRELI 337
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BLAST of mRNA_L-elsbetiae_contig1520.3715.1 vs. uniprot
Match: UPI0006447B19 (hypothetical protein n=1 Tax=Acytostelium subglobosum LB1 TaxID=1410327 RepID=UPI0006447B19)

HSP 1 Score: 94.7 bits (234), Expect = 1.690e-20
Identity = 47/92 (51.09%), Postives = 63/92 (68.48%), Query Frame = 1 
Query:    4 VLRETRGFDVAKGETTHLRRKEGSVDYRFCPEPDLPPLFVADEHVSALKAAMPELPEATMARFKRDYGLPEHLSRLIVCAAGGASGYFEDLV 279
            VLRETRGFD A G+T H+R KE  VDYRF PEPDLPPL ++D+++ +++  M ELP+A  +R  RDY + EH   ++V     A  YFE +V
Sbjct:  270 VLRETRGFDQASGQTFHMRTKEDEVDYRFFPEPDLPPLILSDDYIDSIRNKMVELPDALKSRLMRDYQIAEHEVDVLVEDRDVAQ-YFEAVV 360
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BLAST of mRNA_L-elsbetiae_contig1520.3715.1 vs. uniprot
Match: A0A3R5Y507_9BACT (Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B n=1 Tax=Geovibrio thiophilus TaxID=139438 RepID=A0A3R5Y507_9BACT)

HSP 1 Score: 92.0 bits (227), Expect = 1.430e-19
Identity = 43/94 (45.74%), Postives = 65/94 (69.15%), Query Frame = 1 
Query:    4 VLRETRGFDVAKGETTHLRRKEGSVDYRFCPEPDLPPLFVADEHVSALKAAMPELPEATMARFKRDYGLPEHLSRLIVCAAGGASGYFEDLVNG 285
            +++ETR +D AKG T  +R KE + DYR+ P+PDL P+ ++DE+V A++A +PELP+A MARF  +Y LPEH   + + A    + YFE+ + G
Sbjct:  247 IVQETRLWDAAKGITVSMRSKEEANDYRYFPDPDLVPIILSDEYVEAVRATLPELPDAKMARFMSEYALPEH-DAIALTAEKAYADYFEEALKG 339
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BLAST of mRNA_L-elsbetiae_contig1520.3715.1 vs. uniprot
Match: A0A397C0B5_9STRA (GatB_Yqey domain-containing protein n=2 Tax=Aphanomyces astaci TaxID=112090 RepID=A0A397C0B5_9STRA)

HSP 1 Score: 90.9 bits (224), Expect = 2.520e-19
Identity = 46/95 (48.42%), Postives = 60/95 (63.16%), Query Frame = 1 
Query:    4 VLRETRGFDVAKGETTHLRRKEGSVDYRFCPEPDLPPLFVADEHVSALKAAMPELPEATMARFKRDYGLPEHLSRLIVCAAGGASGYFEDLVNGR 288
            + +ETR FD A G T  +R KEG+ DYRF PEPDLPPL ++   V   KA++PELP+   AR  RDYGL  + S ++V     A  YFE++  GR
Sbjct:  163 IEKETRTFDAATGMTRRMRSKEGAKDYRFFPEPDLPPLVISATFVDDAKASLPELPDQMKARLSRDYGLSAYESAILVHTPH-AVAYFEEVATGR 256
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BLAST of mRNA_L-elsbetiae_contig1520.3715.1 vs. uniprot
Match: A0A3R6Z786_9STRA (Uncharacterized protein (Fragment) n=1 Tax=Aphanomyces astaci TaxID=112090 RepID=A0A3R6Z786_9STRA)

HSP 1 Score: 90.9 bits (224), Expect = 3.010e-19
Identity = 46/95 (48.42%), Postives = 60/95 (63.16%), Query Frame = 1 
Query:    4 VLRETRGFDVAKGETTHLRRKEGSVDYRFCPEPDLPPLFVADEHVSALKAAMPELPEATMARFKRDYGLPEHLSRLIVCAAGGASGYFEDLVNGR 288
            + +ETR FD A G T  +R KEG+ DYRF PEPDLPPL ++   V   KA++PELP+   AR  RDYGL  + S ++V     A  YFE++  GR
Sbjct:  283 IEKETRTFDAATGMTRRMRSKEGAKDYRFFPEPDLPPLVISATFVDDAKASLPELPDQMKARLSRDYGLSAYESAILVHTPH-AVAYFEEVATGR 376
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BLAST of mRNA_L-elsbetiae_contig1520.3715.1 vs. uniprot
Match: A0A554JM02_9BACT (Aspartyl-tRNA(Asn) amidotransferase subunit B / Glutamyl-tRNA(Gln) amidotransferase subunit B (Fragment) n=1 Tax=Parcubacteria group bacterium Gr01-1014_66 TaxID=2017191 RepID=A0A554JM02_9BACT)

HSP 1 Score: 90.5 bits (223), Expect = 3.170e-19
Identity = 45/93 (48.39%), Postives = 63/93 (67.74%), Query Frame = 1 
Query:    4 VLRETRGFDVAKGETTHLRRKEGSVDYRFCPEPDLPPLFVADEHVSALKAAMPELPEATMARFKRDYGLPEHLSRLIVCAAGGASGYFEDLVN 282
            V +ETRG+D  KG T   R KE + DYR+ PEPDLPPL ++DE +  L+A++PELP    ARF R+YGL E +S ++     GA+ YFE +++
Sbjct:  246 VQQETRGWDEQKGITFPQRSKEEAHDYRYFPEPDLPPLEISDELIEELRASLPELPHEKAARFMREYGLDEEMSEILTRDHKGAN-YFESIIS 337
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BLAST of mRNA_L-elsbetiae_contig1520.3715.1 vs. uniprot
Match: W4FIX4_9STRA (Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial n=9 Tax=Aphanomyces astaci TaxID=112090 RepID=W4FIX4_9STRA)

HSP 1 Score: 90.5 bits (223), Expect = 5.510e-19
Identity = 46/95 (48.42%), Postives = 60/95 (63.16%), Query Frame = 1 
Query:    4 VLRETRGFDVAKGETTHLRRKEGSVDYRFCPEPDLPPLFVADEHVSALKAAMPELPEATMARFKRDYGLPEHLSRLIVCAAGGASGYFEDLVNGR 288
            + +ETR FD A G T  +R KEG+ DYRF PEPDLPPL ++   V   KA++PELP+   AR  RDYGL  + S ++V     A  YFE++  GR
Sbjct:  283 IEKETRTFDAATGMTRRMRSKEGAKDYRFFPEPDLPPLVISARFVDDAKASLPELPDQMKARLGRDYGLSAYESAILVHTPH-AVAYFEEVATGR 376
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BLAST of mRNA_L-elsbetiae_contig1520.3715.1 vs. uniprot
Match: UPI001ACC25E8 (Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B n=1 Tax=Burkholderiales bacterium TaxID=1891238 RepID=UPI001ACC25E8)

HSP 1 Score: 89.7 bits (221), Expect = 9.830e-19
Identity = 44/92 (47.83%), Postives = 63/92 (68.48%), Query Frame = 1 
Query:    4 VLRETRGFDVAKGETTHLRRKEGSVDYRFCPEPDLPPLFVADEHVSALKAAMPELPEATMARFKRDYGLPEHLSRLIVCAAGGASGYFEDLV 279
            V++ETR +D  + ET  +R KE + DYR+ P+PDLPPL ++ E ++ +KA MPELPEA  ARF   YGLP+H + ++  +   AS YFE+ V
Sbjct:  258 VVQETRLYDPDRDETRSMRSKEDAQDYRYFPDPDLPPLAISGEWIARVKAGMPELPEAMRARFGTAYGLPDHDAAMLTASRETAS-YFEEAV 348
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig1520.3715.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LEZ1_ECTSI7.060e-4471.68Glutamyl-tRNA(Gln) amidotransferase subunit B, mit... [more]
W7TQ61_9STRA2.260e-2250.00Glutamyl-tRNA(Gln) amidotransferase subunit B, mit... [more]
UPI001999D0CC1.140e-2046.74Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subun... [more]
UPI0006447B191.690e-2051.09hypothetical protein n=1 Tax=Acytostelium subglobo... [more]
A0A3R5Y507_9BACT1.430e-1945.74Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase s... [more]
A0A397C0B5_9STRA2.520e-1948.42GatB_Yqey domain-containing protein n=2 Tax=Aphano... [more]
A0A3R6Z786_9STRA3.010e-1948.42Uncharacterized protein (Fragment) n=1 Tax=Aphanom... [more]
A0A554JM02_9BACT3.170e-1948.39Aspartyl-tRNA(Asn) amidotransferase subunit B / Gl... [more]
W4FIX4_9STRA5.510e-1948.42Glutamyl-tRNA(Gln) amidotransferase subunit B, mit... [more]
UPI001ACC25E89.830e-1947.83Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase s... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig1520contigL-elsbetiae_contig1520:23326..24922 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score159.8
Seed ortholog evalue6.9e-37
Seed eggNOG ortholog2880.D8LEZ1
Preferred namePET112
KEGG rclassRC00010
KEGG koko:K02434
KEGG ReactionR03905,R04212
KEGG Pathwayko00970,ko01100,map00970,map01100
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0006082,GO:0006139,GO:0006399,GO:0006412,GO:0006518,GO:0006520,GO:0006725,GO:0006807,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009987,GO:0010467,GO:0016070,GO:0016874,GO:0016879,GO:0016884,GO:0019538,GO:0019752,GO:0030956,GO:0032543,GO:0032991,GO:0034641,GO:0034645,GO:0034660,GO:0043038,GO:0043039,GO:0043043,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043436,GO:0043603,GO:0043604,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044271,GO:0044281,GO:0044424,GO:0044444,GO:0044464,GO:0046483,GO:0050567,GO:0070681,GO:0071704,GO:0090304,GO:0140053,GO:0140098,GO:0140101,GO:1901360,GO:1901564,GO:1901566,GO:1901576
EggNOG free text desc.Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu- tRNA(Gln)
EggNOG OGsCOG0064@1,KOG2438@2759
EC6.3.5.6,6.3.5.7
COG Functional cat.J
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03029
Ec32 ortholog descriptionAspartyl/glutamyl-tRNA amidotransferase subunit B-related
Ec32 orthologEc-21_006080.1
Exons3
Model size321
Cds size321
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig1520.3715.1prot_L-elsbetiae_contig1520.3715.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig1520 23326..24922 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815619.1370857-CDS-L-elsbetiae_contig1520:23325..233641622815619.1370857-CDS-L-elsbetiae_contig1520:23325..23364Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig1520 23326..23364 +
1691682995.0266674-CDS-L-elsbetiae_contig1520:23325..233641691682995.0266674-CDS-L-elsbetiae_contig1520:23325..23364Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig1520 23326..23364 +
1622815619.1472406-CDS-L-elsbetiae_contig1520:24180..244001622815619.1472406-CDS-L-elsbetiae_contig1520:24180..24400Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig1520 24181..24400 +
1691682995.0394635-CDS-L-elsbetiae_contig1520:24180..244001691682995.0394635-CDS-L-elsbetiae_contig1520:24180..24400Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig1520 24181..24400 +
1622815619.156466-CDS-L-elsbetiae_contig1520:24860..249221622815619.156466-CDS-L-elsbetiae_contig1520:24860..24922Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig1520 24861..24922 +
1691682995.0563886-CDS-L-elsbetiae_contig1520:24860..249221691682995.0563886-CDS-L-elsbetiae_contig1520:24860..24922Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig1520 24861..24922 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig1520.3715.1

>prot_L-elsbetiae_contig1520.3715.1 ID=prot_L-elsbetiae_contig1520.3715.1|Name=mRNA_L-elsbetiae_contig1520.3715.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=107bp
GVLRETRGFDVAKGETTHLRRKEGSVDYRFCPEPDLPPLFVADEHVSALK
AAMPELPEATMARFKRDYGLPEHLSRLIVCAAGGASGYFEDLVNGRTGEG
REGLRRD
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mRNA from alignment at L-elsbetiae_contig1520:23326..24922+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig1520.3715.1 ID=mRNA_L-elsbetiae_contig1520.3715.1|Name=mRNA_L-elsbetiae_contig1520.3715.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=1597bp|location=Sequence derived from alignment at L-elsbetiae_contig1520:23326..24922+ (Laminarionema elsbetiae ELsaHSoW15)
GGGGTCCTGAGGGAGACGAGGGGCTTCGACGTGGCCAAGGTGTGCCAAGG
TTGGTTTTAATGCTTGCGTGGTGGGGTAGGGTGTACGTGCGGATTACAAA
TAACTCGTTCGTTTCCTTGTGTGCCGGCCAGGATTGTTCCTTGCGGCGTT
CCGCGTGATAGGTCAGGTCGAGTTGTTGCACCGCTTTCAGTTTACATGGG
GTCGTGCGCTTCGTGAGAGAGGCAGACCACTGACAGTGCTCGAGCGGACA
GGTTCTAGATCTGCACCCCCACCTGTAAGTCTAGTGTGTAAGTACGTATG
TATGTATGTATCGCGGCCATTGCGAAGAACGGGCTTCGCCCGCGACGCTT
TCGGCGCGAAACATCGACGTCTGCTTTGCTCACGAGTTCGGAGCACGTTT
ATCACGGGCAAGCTATATATATATCTGCGCCTCGCGCGTGTAACTCGTGT
CACAAGAGGTATATGGGCATACTCCCGTTTTCGCTGCTTGTTCGGCATGG
CCATCTACCTTCCCGAGCCCGAAACCACGCAGCGAAAAATTGCTGCTTTC
CTCCGCGCTAATTGCCAGCGCTCAAGCAGCGTACATTTCTACCCTCCAAG
GAAGATGATTGCGGCAGTTACATAGCGTCCATTACACCACCGTGGCTCGC
AGAAGCCCGTCTTCTCTCTTTTCTGGGCCGCTGCGGTGCAATACAATGCA
TAGGCTAGCCAATAGCAGCACAGCAGGTGATGCACATCATACCGAGCCGA
CGGGGGGCTGCGCGCATGCACGAACCTACATCATACCCGGCCTGGAACCC
TCATAAAACCATACCCCCCTGAAAAAACTCGACAACGACGACAACCGGCG
ACCAGGGTGAAACGACTCATCTCCGTCGCAAGGAGGGCTCGGTGGACTAC
CGCTTTTGCCCGGAGCCGGATCTGCCTCCGCTTTTCGTCGCCGACGAGCA
CGTTAGCGCCCTCAAGGCGGCCATGCCCGAGCTGCCCGAGGCGACGATGG
CCAGGTTTAAGAGGGACTACGGGCTACCGGAGCACCTGAGCCGGCTCATC
GTGTGTGCTGCAGGGGGGGCATCAGGTGCGCTAATTTACCGCCGCCGCCG
CCGGGTGTGCGCGTGTGGTGTCGCACGTGCACCTGCGCTTTTCCATCGTG
ACTCGTTTTTTTTTTTATTGTAGCCGTGCACAAATTTTGAGGCGGCTGGC
TGGTGTTGTTGCTTGGTCGGGCACGTAGATTGATTTTCTCCCCCCGCCCC
GTTCGAAGCACCTGGGGTGTGCAGAGTTGTCTTTGCACCAGCGCGTGCAG
CATGGGCGTTCCCTCCCCGAGCCGCAGATTTACTCTCCAAGGTCAGAGAG
GCGGTATACCTTGACGAAGGAGGTTTGGGAGTCCAAGAAGTGGGCCTGGG
CCGTCAAAAGAGCGACTTTAACTCCATCCAACAAGTAGCAAACACCAGTA
AACAGCAACAACAACACTAATTAACAACCTCGGCAACCGTCGCACCGGTG
CTTCTAAACGGCCTTGCGAACCGACGCCCACCCAGGCTATTTCGAGGACC
TCGTCAACGGTAGAACAGGGGAGGGGCGGGAAGGGCTTCGAAGGGAT
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Coding sequence (CDS) from alignment at L-elsbetiae_contig1520:23326..24922+

>mRNA_L-elsbetiae_contig1520.3715.1 ID=mRNA_L-elsbetiae_contig1520.3715.1|Name=mRNA_L-elsbetiae_contig1520.3715.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=642bp|location=Sequence derived from alignment at L-elsbetiae_contig1520:23326..24922+ (Laminarionema elsbetiae ELsaHSoW15)
GGGGTCCTGAGGGAGACGAGGGGCTTCGACGTGGCCAAGGGGGTCCTGAG
GGAGACGAGGGGCTTCGACGTGGCCAAGGGTGAAACGACTCATCTCCGTC
GCAAGGAGGGCTCGGTGGACTACCGCTTTTGCCCGGAGCCGGATCTGCCT
CCGCTTTTCGTCGCCGACGAGCACGTTAGCGCCCTCAAGGCGGCCATGCC
CGAGCTGCCCGAGGCGACGATGGCCAGGTTTAAGAGGGACTACGGGCTAC
CGGAGCACCTGAGCCGGCTCATCGTGTGTGCTGCAGGGGGGGCATCAGGG
TGAAACGACTCATCTCCGTCGCAAGGAGGGCTCGGTGGACTACCGCTTTT
GCCCGGAGCCGGATCTGCCTCCGCTTTTCGTCGCCGACGAGCACGTTAGC
GCCCTCAAGGCGGCCATGCCCGAGCTGCCCGAGGCGACGATGGCCAGGTT
TAAGAGGGACTACGGGCTACCGGAGCACCTGAGCCGGCTCATCGTGTGTG
CTGCAGGGGGGGCATCAGGCTATTTCGAGGACCTCGTCAACGGTAGAACA
GGGGAGGGGCGGGAAGGGCTTCGAAGGGATGCTATTTCGAGGACCTCGTC
AACGGTAGAACAGGGGAGGGGCGGGAAGGGCTTCGAAGGGAT
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