mRNA_L-elsbetiae_contig19997.5992.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig19997.5992.1
Unique NamemRNA_L-elsbetiae_contig19997.5992.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
BLAST of mRNA_L-elsbetiae_contig19997.5992.1 vs. uniprot
Match: D8LDU2_ECTSI (DNA topoisomerase (Fragment) n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LDU2_ECTSI)

HSP 1 Score: 142 bits (358), Expect = 1.380e-36
Identity = 108/158 (68.35%), Postives = 113/158 (71.52%), Query Frame = 1
Query:    1 GDAKVAKVKGSQEAHEAIRPALVGASEG-----------EEEGXXXXXXXXXXXXXAGTRGAATGVGVGRFRHPSELPL--DGG----LEDQQRALYDLVYRRTLASAMAESEADFTTVRLGAAG-----GGGVVDVVLKATGKTTVFEGFLKAYREE 408
            GDAK  KVKGSQEAHEAIRPALVGA+             EEE XXXXXXXXXXXX  G          GRF HP+ELP+  DGG    LE+Q RALYDLVYRRTLASAMAESEADFTTV LGA G     G  VVDVVLKA+GKTTVFEGFLKAYREE
Sbjct:  457 GDAKTVKVKGSQEAHEAIRPALVGATPAATDTADGKQAREEEXXXXXXXXXXXXXTTG----------GRFLHPTELPVEEDGGGGRGLEEQHRALYDLVYRRTLASAMAESEADFTTVSLGAGGVSLGDGEEVVDVVLKASGKTTVFEGFLKAYREE 604          
BLAST of mRNA_L-elsbetiae_contig19997.5992.1 vs. uniprot
Match: A0A6H5KCV2_9PHAE (TOP1Ac domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KCV2_9PHAE)

HSP 1 Score: 138 bits (348), Expect = 2.710e-35
Identity = 102/156 (65.38%), Postives = 112/156 (71.79%), Query Frame = 1
Query:    1 GDAKVAKVKGSQEAHEAIRPALVGAS-------EGEEEGXXXXXXXXXXXXXAGTRGAATGVGVGRFRHPSELPLDG--------GLEDQQRALYDLVYRRTLASAMAESEADFTTVRLGAAG-----GGGVVDVVLKATGKTTVFEGFLKAYREE 408
            GDAKV KVKGSQEAHEAIRPALVGA+       +G++ G  XXXXXXXXXXX            GRF HP+ELP++         GLE+Q RALYDLVYRRTLASAMAESEADFTTV LGA G     G  VVDVVLKA+GKTTVF+GFLKAYR E
Sbjct:  158 GDAKVVKVKGSQEAHEAIRPALVGATPAATDTHDGKQAGEEXXXXXXXXXXXXXXXXXG-----GRFLHPTELPVEEXXXXXXXRGLEEQHRALYDLVYRRTLASAMAESEADFTTVSLGAGGVSLGDGEEVVDVVLKASGKTTVFDGFLKAYRVE 308          
BLAST of mRNA_L-elsbetiae_contig19997.5992.1 vs. uniprot
Match: D7G724_ECTSI (TOP1Ac domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G724_ECTSI)

HSP 1 Score: 104 bits (259), Expect = 2.620e-24
Identity = 73/139 (52.52%), Postives = 77/139 (55.40%), Query Frame = 1
Query:    1 GDAKVAKVKGSQEAHEAIRPALVGASEGEEEGXXXXXXXXXXXXXAGTRGAATGVGVGRFRHPSELPLDGGLEDQQRALYDLVYRRTLASAMAESEADFTTVRLGAAG-----GGGVVDVVLKATGKTTVFEGFLKAYR 402
            GDAK  KVKGSQEAHEAIRPAL      +  G             AG                        LE+Q RA YDLVYRRTLASAMAESEAD TTV LGA G     G  VVDVVLKA+G TT+FEGFLK  R
Sbjct:  111 GDAKAVKVKGSQEAHEAIRPAL------QVVGAVPAATDTPDGNPAGGEEXXXXXXXXXXXXXXXXXXXXXLEEQHRAPYDLVYRRTLASAMAESEADLTTVSLGAGGVSLGDGEEVVDVVLKASGMTTLFEGFLKISR 243          
BLAST of mRNA_L-elsbetiae_contig19997.5992.1 vs. uniprot
Match: A0A845LY03_9RHOB (DNA topoisomerase 1 n=1 Tax=Maritimibacter harenae TaxID=2606218 RepID=A0A845LY03_9RHOB)

HSP 1 Score: 66.2 bits (160), Expect = 6.050e-10
Identity = 38/82 (46.34%), Postives = 54/82 (65.85%), Query Frame = 1
Query:  187 PSELPLDGG----LEDQQRALYDLVYRRTLASAMAESEADFTTVRLGAAGGGGVVDVVLKATGKTTVFEGFLKAYREETDED 420
            P+++ LD G    LE  QR LYDL+++RTLA  MA ++ + TTV +G+A G     V L+ATG+  +F+GFLK Y E  D+D
Sbjct:  348 PTDMSLDAGQLGKLEADQRKLYDLIWKRTLACQMAGAKLERTTVEIGSADG----QVGLRATGQVVLFDGFLKVYEEGRDDD 425          
BLAST of mRNA_L-elsbetiae_contig19997.5992.1 vs. uniprot
Match: UPI00048C4BFA (type I DNA topoisomerase n=1 Tax=Prevotella sp. HUN102 TaxID=1392486 RepID=UPI00048C4BFA)

HSP 1 Score: 65.1 bits (157), Expect = 1.520e-9
Identity = 35/68 (51.47%), Postives = 48/68 (70.59%), Query Frame = 1
Query:  223 QQRALYDLVYRRTLASAMAESEADFTTVRLGAAGGGGVVDVVLK--ATGKTTVFEGFLKAYREETDED 420
            Q++ LYDL+++RT+AS MA++  D TTV +G   G G  D  L+  ATG+  VF+GFLK YRE TD+D
Sbjct:  350 QEKRLYDLIWKRTVASQMADARIDKTTVNIGIIDGNGKSDDSLQFIATGEVIVFDGFLKVYRESTDDD 417          
BLAST of mRNA_L-elsbetiae_contig19997.5992.1 vs. uniprot
Match: A0A521XHW9_9ACTN (DNA topoisomerase 1 n=1 Tax=Actinomycetia bacterium TaxID=1883427 RepID=A0A521XHW9_9ACTN)

HSP 1 Score: 65.1 bits (157), Expect = 1.560e-9
Identity = 40/81 (49.38%), Postives = 50/81 (61.73%), Query Frame = 1
Query:  178 FRHPSELPLDGGLEDQQRALYDLVYRRTLASAMAESEADFTTVRLGAAGGGGVVDVVLKATGKTTVFEGFLKAYREETDED 420
            FR P+++   G L     ALYDL+++RT+AS MA++    TTVRLGA    GV D    A+G   VF GFL AY E TDED
Sbjct:  528 FRTPAQVA--GELRGDDFALYDLIWKRTVASQMADARVSTTTVRLGAQTAAGV-DAEFAASGTVIVFAGFLAAYEEGTDED 605          
BLAST of mRNA_L-elsbetiae_contig19997.5992.1 vs. uniprot
Match: A0A8I1IYN8_9RHOB (DNA topoisomerase 1 n=1 Tax=Rhodobacteraceae bacterium TaxID=1904441 RepID=A0A8I1IYN8_9RHOB)

HSP 1 Score: 64.3 bits (155), Expect = 2.840e-9
Identity = 38/81 (46.91%), Postives = 52/81 (64.20%), Query Frame = 1
Query:  187 PSELPLDGG----LEDQQRALYDLVYRRTLASAMAESEADFTTVRLGAAGGGGVVDVVLKATGKTTVFEGFLKAYREETDE 417
            P+++  D G    LE  QR LYDL+++RTLAS MA +  + TTV +GAA G     V L+A+G+  +F+GFLK Y E  DE
Sbjct:  348 PTDMGRDAGALGRLEADQRKLYDLIWKRTLASQMAAARLERTTVEIGAADG----QVGLRASGQVVLFDGFLKVYEEGRDE 424          
BLAST of mRNA_L-elsbetiae_contig19997.5992.1 vs. uniprot
Match: A0A356V4U8_9RHOB (DNA topoisomerase (Fragment) n=1 Tax=Rhodobacteraceae bacterium TaxID=1904441 RepID=A0A356V4U8_9RHOB)

HSP 1 Score: 63.9 bits (154), Expect = 3.750e-9
Identity = 35/81 (43.21%), Postives = 54/81 (66.67%), Query Frame = 1
Query:  187 PSELPLDGG----LEDQQRALYDLVYRRTLASAMAESEADFTTVRLGAAGGGGVVDVVLKATGKTTVFEGFLKAYREETDE 417
            P+++ +D G    LE  QR LYDL+++RTLAS M +++ + TTV +G+  G     VVL+ATG+  +F+GF+K Y E  D+
Sbjct:  348 PTDMSVDAGKLARLEADQRKLYDLIWKRTLASQMEQAKLERTTVDMGSEDG----QVVLRATGQVVLFDGFMKVYEEGRDD 424          
BLAST of mRNA_L-elsbetiae_contig19997.5992.1 vs. uniprot
Match: A0A133QHS1_9BACT (DNA topoisomerase 1 n=3 Tax=Prevotella corporis TaxID=28128 RepID=A0A133QHS1_9BACT)

HSP 1 Score: 63.5 bits (153), Expect = 5.230e-9
Identity = 32/68 (47.06%), Postives = 48/68 (70.59%), Query Frame = 1
Query:  223 QQRALYDLVYRRTLASAMAESEADFTTVRLGAAG--GGGVVDVVLKATGKTTVFEGFLKAYREETDED 420
            Q++ LYDL+++RT+AS MA+++ D TTV +G  G  GG   D+   ATG+   F+GFLK YRE T+++
Sbjct:  355 QEKRLYDLIWKRTIASQMADAKIDKTTVNIGIVGEEGGDAGDLQFVATGEVVAFDGFLKVYRESTEDE 422          
BLAST of mRNA_L-elsbetiae_contig19997.5992.1 vs. uniprot
Match: UPI001F5AADFD (type I DNA topoisomerase n=1 Tax=Aliiroseovarius sp. N1Y82 TaxID=2925840 RepID=UPI001F5AADFD)

HSP 1 Score: 63.5 bits (153), Expect = 5.270e-9
Identity = 36/81 (44.44%), Postives = 53/81 (65.43%), Query Frame = 1
Query:  187 PSELPLDGG----LEDQQRALYDLVYRRTLASAMAESEADFTTVRLGAAGGGGVVDVVLKATGKTTVFEGFLKAYREETDE 417
            P+++ +D      LE  QR LYDL+++RTLAS M  +  + T+V +G+A G    +VVL+ATG+  +FEGFLK Y E  D+
Sbjct:  348 PTDMTVDASALARLEPDQRKLYDLIWKRTLASQMESARLERTSVDVGSADG----EVVLRATGQVVLFEGFLKVYEEGRDD 424          
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig19997.5992.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LDU2_ECTSI1.380e-3668.35DNA topoisomerase (Fragment) n=1 Tax=Ectocarpus si... [more]
A0A6H5KCV2_9PHAE2.710e-3565.38TOP1Ac domain-containing protein n=1 Tax=Ectocarpu... [more]
D7G724_ECTSI2.620e-2452.52TOP1Ac domain-containing protein n=1 Tax=Ectocarpu... [more]
A0A845LY03_9RHOB6.050e-1046.34DNA topoisomerase 1 n=1 Tax=Maritimibacter harenae... [more]
UPI00048C4BFA1.520e-951.47type I DNA topoisomerase n=1 Tax=Prevotella sp. HU... [more]
A0A521XHW9_9ACTN1.560e-949.38DNA topoisomerase 1 n=1 Tax=Actinomycetia bacteriu... [more]
A0A8I1IYN8_9RHOB2.840e-946.91DNA topoisomerase 1 n=1 Tax=Rhodobacteraceae bacte... [more]
A0A356V4U8_9RHOB3.750e-943.21DNA topoisomerase (Fragment) n=1 Tax=Rhodobacterac... [more]
A0A133QHS1_9BACT5.230e-947.06DNA topoisomerase 1 n=3 Tax=Prevotella corporis Ta... [more]
UPI001F5AADFD5.270e-944.44type I DNA topoisomerase n=1 Tax=Aliiroseovarius s... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig19997contigL-elsbetiae_contig19997:148..974 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score144.8
Seed ortholog evalue3e-32
Seed eggNOG ortholog2880.D8LDU2
KEGG koko:K03168
Hectar predicted targeting categoryother localisation
EggNOG free text desc.DNA topoisomerase type I activity
EggNOG OGsCOG0550@1,KOG1956@2759
EC5.99.1.2
COG Functional cat.L
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko03032,ko03400
Ec32 ortholog descriptionDNA topoisomerase I
Ec32 orthologEc-08_003340.1
Exons2
Model size422
Cds size420
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815825.5416746-CDS-L-elsbetiae_contig19997:147..4801622815825.5416746-CDS-L-elsbetiae_contig19997:147..480Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig19997 148..480 +
1691683127.6041067-CDS-L-elsbetiae_contig19997:147..4801691683127.6041067-CDS-L-elsbetiae_contig19997:147..480Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig19997 148..480 +
1622815825.5549588-CDS-L-elsbetiae_contig19997:885..9721622815825.5549588-CDS-L-elsbetiae_contig19997:885..972Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig19997 886..972 +
1691683127.617108-CDS-L-elsbetiae_contig19997:885..9721691683127.617108-CDS-L-elsbetiae_contig19997:885..972Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig19997 886..972 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig19997.5992.1prot_L-elsbetiae_contig19997.5992.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig19997 148..972 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig19997.5992.1

>prot_L-elsbetiae_contig19997.5992.1 ID=prot_L-elsbetiae_contig19997.5992.1|Name=mRNA_L-elsbetiae_contig19997.5992.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=140bp
GDAKVAKVKGSQEAHEAIRPALVGASEGEEEGGGDEDTGAAAAGGAGTRG
AATGVGVGRFRHPSELPLDGGLEDQQRALYDLVYRRTLASAMAESEADFT
TVRLGAAGGGGVVDVVLKATGKTTVFEGFLKAYREETDED
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mRNA from alignment at L-elsbetiae_contig19997:148..974+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig19997.5992.1 ID=mRNA_L-elsbetiae_contig19997.5992.1|Name=mRNA_L-elsbetiae_contig19997.5992.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=827bp|location=Sequence derived from alignment at L-elsbetiae_contig19997:148..974+ (Laminarionema elsbetiae ELsaHSoW15)
GGAGACGCGAAGGTGGCCAAGGTAAAAGGAAGCCAGGAGGCACACGAAGC CATCCGCCCGGCCCTAGTCGGTGCTTCCGAGGGGGAAGAGGAGGGGGGGG GCGACGAGGACACAGGCGCGGCGGCCGCCGGAGGCGCGGGGACAAGGGGG GCGGCGACGGGGGTGGGGGTGGGGAGGTTCCGCCACCCGTCGGAGCTCCC TCTGGACGGCGGGCTGGAAGACCAGCAGAGGGCGCTGTACGACCTGGTGT ACCGGAGGACGCTGGCCAGCGCAATGGCGGAGTCGGAGGCGGACTTCACG ACGGTCAGGCTCGGAGCGGCGGGCGGCGGGGGCGTCTCGCTGGGAGGGGA TGACGAGGTAGGTCTGTCCGTTGCTGCTGTTGTTGGTTGGTGTTGGTGTT GTTGGTGTTGGTGGTGGTAGTGGTGGTGGTGGTGGTGTGGTGTGGTAAAT GCACCTAAGTCTATGTAGAGGGTTAGTCGTTCATATCTACTTCTTGCAAC ACATACATGTGTATTTACATCGGTGTTTTTTATGTTGTTGTTGTTGCTGT TGCTGTTGCTTTTGCTTTCGGTGTTCTTTGGGTAGATGCATTAAGTCTAT CGTTCATACAATATCTACTTCCTTGTACTAATGTATACCATACATACACA CAACGTACCATGAGGTGGTTTCTATTGTAAAACAAAACAACCGTATTTAC CTGTTTGTTTGTTTTTTCGTTTTGCCACCACCGCACAGGTGGTCGATGTT GTGCTGAAGGCGACCGGGAAGACGACGGTTTTCGAAGGGTTCTTGAAGGC GTACAGGGAGGAGACGGACGAAGATCT
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Coding sequence (CDS) from alignment at L-elsbetiae_contig19997:148..974+

>mRNA_L-elsbetiae_contig19997.5992.1 ID=mRNA_L-elsbetiae_contig19997.5992.1|Name=mRNA_L-elsbetiae_contig19997.5992.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=840bp|location=Sequence derived from alignment at L-elsbetiae_contig19997:148..974+ (Laminarionema elsbetiae ELsaHSoW15)
GGAGACGCGAAGGTGGCCAAGGTAAAAGGAAGCCAGGAGGCACACGAAGC
CATCCGCCCGGCCCTAGTCGGTGCTTCCGAGGGGGAAGAGGAGGGGGGGG
GCGACGAGGACACAGGCGCGGCGGCCGCCGGAGGCGCGGGGACAAGGGGG
GCGGCGACGGGGGTGGGGGTGGGGAGGTTCCGCCACCCGTCGGAGCTCCC
TCTGGACGGCGGGCTGGAAGACCAGCAGAGGGCGCTGTACGACCTGGTGT
ACCGGAGGACGCTGGCCAGCGCAATGGCGGAGTCGGAGGCGGACTTCACG
ACGGTCAGGCTCGGAGCGGCGGGCGGCGGGGGCGGAGACGCGAAGGTGGC
CAAGGTAAAAGGAAGCCAGGAGGCACACGAAGCCATCCGCCCGGCCCTAG
TCGGTGCTTCCGAGGGGGAAGAGGAGGGGGGGGGCGACGAGGACACAGGC
GCGGCGGCCGCCGGAGGCGCGGGGACAAGGGGGGCGGCGACGGGGGTGGG
GGTGGGGAGGTTCCGCCACCCGTCGGAGCTCCCTCTGGACGGCGGGCTGG
AAGACCAGCAGAGGGCGCTGTACGACCTGGTGTACCGGAGGACGCTGGCC
AGCGCAATGGCGGAGTCGGAGGCGGACTTCACGACGGTCAGGCTCGGAGC
GGCGGGCGGCGGGGGCGTGGTCGATGTTGTGCTGAAGGCGACCGGGAAGA
CGACGGTTTTCGAAGGGTTCTTGAAGGCGTACAGGGAGGAGACGGACGAA
GATGTGGTCGATGTTGTGCTGAAGGCGACCGGGAAGACGACGGTTTTCGA
AGGGTTCTTGAAGGCGTACAGGGAGGAGACGGACGAAGAT
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