mRNA_L-elsbetiae_contig824.17247.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig824.17247.1
Unique NamemRNA_L-elsbetiae_contig824.17247.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
BLAST of mRNA_L-elsbetiae_contig824.17247.1 vs. uniprot
Match: D8LCL1_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LCL1_ECTSI)

HSP 1 Score: 147 bits (371), Expect = 1.230e-40
Identity = 75/126 (59.52%), Postives = 92/126 (73.02%), Query Frame = 2
Query:    2 AAFSAPPDEDEHHLVFLGLLGGTGQPLGMLTARAQFSVSGGRRAEREVGPLQAATNGRVYGTVQYPSMKELWPDIYTTPGVRAVEVNLCVEVRPLHRKVRYLQVDFKVMYRRGGVGT-SQGALWRP 376
            AAFS P  +DE HLVF+GL G TG+PL  L ARAQFS + GR+AER VGPLQA +NGRVYG VQYPSM ELWP++Y +  +R V+V++ VEVR  +    YL  + K MYRR   G+ +QGALWRP
Sbjct:  228 AAFSVPRSDDEQHLVFIGL-GETGRPLPTLAARAQFSANPGRKAERMVGPLQAGSNGRVYGAVQYPSMAELWPEVYNSTAIRVVKVDMLVEVRSRYGDSLYLATELKAMYRRPPGGSRNQGALWRP 352          
BLAST of mRNA_L-elsbetiae_contig824.17247.1 vs. uniprot
Match: A0A6H5JZ42_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JZ42_9PHAE)

HSP 1 Score: 134 bits (338), Expect = 4.150e-35
Identity = 72/128 (56.25%), Postives = 89/128 (69.53%), Query Frame = 2
Query:    2 AAFSAPPDEDEHHLVFLGLLGGTGQPLGMLTARAQFSVSGGRRAEREVGPLQA--ATNGRVYGTVQYPSMKELWPDIYTTPGVRAVEVNLCVEVRPLHRKVRYLQVDFKVMYRRGGVGT-SQGALWRP 376
            AA S P  +DE +LVF+GL G TG+PL  L ARAQFS + GR+AER VGPLQA   +NGRVYG VQYPSM ELWP++Y +  +R V+V++ VEVR       YL  +   MYRR   G+ +QGALWRP
Sbjct:  310 AALSVPRSDDEQYLVFIGL-GETGRPLPALAARAQFSANPGRQAERMVGPLQARAGSNGRVYGAVQYPSMAELWPEVYNSKAIRVVKVDMLVEVRSRSGDSLYLATELMAMYRRPSGGSRNQGALWRP 436          
BLAST of mRNA_L-elsbetiae_contig824.17247.1 vs. uniprot
Match: D7G6J1_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G6J1_ECTSI)

HSP 1 Score: 91.3 bits (225), Expect = 7.490e-21
Identity = 46/102 (45.10%), Postives = 61/102 (59.80%), Query Frame = 2
Query:   32 EHHLVFLGLLGGTGQPLGMLTARAQFSVSGGRRAEREVGPLQAATNGRVYGTVQYPSMKELWPDIYTTPGVRAVEVNLCVEVRPLHRKVRYLQVDFKVMYRR 337
            E HLVF GL+G  G  L  LT    FS++ GR   R  GP+Q   + RVY  V+YP+M ELWP+IY    V  V+V++ + VR  H+ +RY   + KV YRR
Sbjct:   35 EQHLVFTGLVG-AGPHLDYLTTTVYFSINSGRPLARAAGPMQVGEDERVYVAVKYPTMNELWPEIYQNVAVEVVQVDVFITVRSGHKTIRYPGTELKVTYRR 135          
BLAST of mRNA_L-elsbetiae_contig824.17247.1 vs. uniprot
Match: D7FZ48_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FZ48_ECTSI)

HSP 1 Score: 83.2 bits (204), Expect = 4.970e-16
Identity = 43/100 (43.00%), Postives = 58/100 (58.00%), Query Frame = 2
Query:   38 HLVFLGLLGGTGQPLGMLTARAQFSVSGGRRAEREVGPLQAATNGRVYGTVQYPSMKELWPDIYTTPGVRAVEVNLCVEVRPLHRKVRYLQVDFKVMYRR 337
            HLVF GL   TGQ L  L   A F V    +  +   P+Q  +NGR Y  V YPSM+E+WP++Y+T  +R VEV+L VE+R    + +Y+    KV Y R
Sbjct:  412 HLVFTGL-NNTGQDLRSLRVTAHFGVDSVHKVGKVTSPIQLGSNGRAYAGVFYPSMQEIWPELYSTSNIRVVEVDLVVEIRSDSGEYQYVGPQLKVTYLR 510          
BLAST of mRNA_L-elsbetiae_contig824.17247.1 vs. uniprot
Match: D7G8W7_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G8W7_ECTSI)

HSP 1 Score: 78.6 bits (192), Expect = 8.020e-16
Identity = 41/81 (50.62%), Postives = 53/81 (65.43%), Query Frame = 2
Query:  137 REVGPLQAATNGRVYGTVQYPSMKELWPDIYTTPGVRAVEVNLCVEVRPLHRKVRYLQVDFKVMYRRG-GVGTSQGALWRP 376
            RE GP     NGRVYG VQ PSMK+LWP++Y+   VR V+V+L VE+R       +L  + KV+YRR    G+S+GA WRP
Sbjct:   85 REAGP-----NGRVYGGVQCPSMKDLWPEVYSAAAVRVVQVDLRVEIRSRSGDFLHLDSELKVIYRRPQDHGSSEGASWRP 160          
BLAST of mRNA_L-elsbetiae_contig824.17247.1 vs. uniprot
Match: A0A6H5L3B8_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L3B8_9PHAE)

HSP 1 Score: 72.0 bits (175), Expect = 1.520e-13
Identity = 36/78 (46.15%), Postives = 46/78 (58.97%), Query Frame = 2
Query:   32 EHHLVFLGLLGGTGQPLGMLTARAQFSVSGGRRAEREVGPLQAATNGRVYGTVQYPSMKELWPDIYTTPGVRAVEVNL 265
            E HLVF GL+G  G  L  LT    FS++ GR   R  GP+Q   + RVY  V++P+M ELWP+IY    V  V+V L
Sbjct:   44 EQHLVFTGLVG-AGPDLDYLTTTVYFSINSGRPLARAAGPMQVGEDERVYVAVKFPTMNELWPEIYQNVAVEVVQVRL 120          
BLAST of mRNA_L-elsbetiae_contig824.17247.1 vs. uniprot
Match: D7G7N4_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G7N4_ECTSI)

HSP 1 Score: 63.2 bits (152), Expect = 9.980e-10
Identity = 39/97 (40.21%), Postives = 52/97 (53.61%), Query Frame = 2
Query:   38 HLVFLGLLGGTGQPLGMLTARAQFSVSGGRRAEREVGPLQAATNGRVYGTVQYPSMKELWPDIYTTPGVRAVEVNLCVEVRPLH-RKVRYLQVDFKV 325
            H+VF GL      P G L     F V    +      P+Q A +GR Y  V YPS++E+WP++Y T  VRAVEV++ VEV     R+ RY+    KV
Sbjct:   84 HVVFTGLNDTGHDPRG-LRVTVIFGVDAVHQIACVTSPIQVAADGRAYSRVFYPSLEEVWPELYYTVDVRAVEVDVAVEVLSDDGREFRYVGPHLKV 179          
BLAST of mRNA_L-elsbetiae_contig824.17247.1 vs. uniprot
Match: A0A6H5JDQ6_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JDQ6_9PHAE)

HSP 1 Score: 63.9 bits (154), Expect = 2.530e-9
Identity = 39/101 (38.61%), Postives = 54/101 (53.47%), Query Frame = 2
Query:   38 HLVFLGLLGGTGQPLGMLTARAQFSVSGGRRAEREVGPLQAATNGRVYGTVQYPSMKELWPDIYTTPGVRAVEVNLCVEVRPLH-RKVRYLQVDFKVMYRR 337
            H+VF GL      P G L     F V    +      P+Q A +GR Y  V YPS++++WP++Y T  VRAVEV++ VE+     R+ RY+    KV Y R
Sbjct:  262 HVVFTGLHNTGHDPRG-LRVTVIFGVDAVHQIACVTSPVQVAADGRAYSRVFYPSLEDVWPELYYTVDVRAVEVDVAVEILSDDGREFRYVGPHLKVGYTR 361          
BLAST of mRNA_L-elsbetiae_contig824.17247.1 vs. uniprot
Match: D8LCK7_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LCK7_ECTSI)

HSP 1 Score: 58.2 bits (139), Expect = 2.840e-7
Identity = 35/106 (33.02%), Postives = 48/106 (45.28%), Query Frame = 2
Query:   92 TARAQFSVSGGRRAEREVG-PLQAATN-----------------GRVYGTVQYPSMKELWPDIYTTPGVRAVEVNLCVEVRPLHRKVRYLQVDFKVMYRRGGVGTS 355
            T  A+FSV    R ER    PL  A                   GRV   V++PS+ E+WP++Y    +R +EV++ VEVR     + Y     K+ YRR    TS
Sbjct:  524 TVCARFSVDPASRVERFASLPLDGAAGXXXXXXXDPAGAVGGRRGRVDAAVEFPSIMEIWPEVYKVDNIRVMEVDMVVEVRSSDGNILYTGPTLKITYRRSAARTS 629          
BLAST of mRNA_L-elsbetiae_contig824.17247.1 vs. uniprot
Match: A0A6H5K0L1_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K0L1_9PHAE)

HSP 1 Score: 53.9 bits (128), Expect = 8.560e-6
Identity = 34/93 (36.56%), Postives = 48/93 (51.61%), Query Frame = 2
Query:   89 LTARAQFSVSG-GRRAEREVGPLQAATN---GRVYGTVQYPSMKELWPDIYTTPGVRAVEVNLCVEVRPLHRKVRYLQVDFKVMYRRGGVGTS 355
            L   AQFSV   G         L+A  N   G    T+ YPSM+++WP++Y+   V+ VEV++ V+VR    K  +     KVMYRR    T+
Sbjct:  487 LAVTAQFSVGPPGNLVGTAPTNLEADRNACAGSSSCTITYPSMEDIWPEVYSARSVKVVEVDMTVDVRSSDGKWVHSWPSVKVMYRRARTATT 579          
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig824.17247.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 10
Match NameE-valueIdentityDescription
D8LCL1_ECTSI1.230e-4059.52Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5JZ42_9PHAE4.150e-3556.25Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7G6J1_ECTSI7.490e-2145.10Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
D7FZ48_ECTSI4.970e-1643.00Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
D7G8W7_ECTSI8.020e-1650.62Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5L3B8_9PHAE1.520e-1346.15Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7G7N4_ECTSI9.980e-1040.21Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5JDQ6_9PHAE2.530e-938.61Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D8LCK7_ECTSI2.840e-733.02Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5K0L1_9PHAE8.560e-636.56Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig824contigL-elsbetiae_contig824:27573..29757 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Hectar predicted targeting categoryother localisation
Exons4
Model size394
Cds size393
Stop1
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig824.17247.1prot_L-elsbetiae_contig824.17247.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig824 27573..29757 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816907.3278394-CDS-L-elsbetiae_contig824:27572..277291622816907.3278394-CDS-L-elsbetiae_contig824:27572..27729Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig824 27573..27729 +
1691683903.1702645-CDS-L-elsbetiae_contig824:27572..277291691683903.1702645-CDS-L-elsbetiae_contig824:27572..27729Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig824 27573..27729 +
1622816907.3419936-CDS-L-elsbetiae_contig824:27886..279851622816907.3419936-CDS-L-elsbetiae_contig824:27886..27985Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig824 27887..27985 +
1691683903.1795945-CDS-L-elsbetiae_contig824:27886..279851691683903.1795945-CDS-L-elsbetiae_contig824:27886..27985Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig824 27887..27985 +
1622816907.355646-CDS-L-elsbetiae_contig824:28806..288721622816907.355646-CDS-L-elsbetiae_contig824:28806..28872Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig824 28807..28872 +
1691683903.1883166-CDS-L-elsbetiae_contig824:28806..288721691683903.1883166-CDS-L-elsbetiae_contig824:28806..28872Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig824 28807..28872 +
1622816907.3686538-CDS-L-elsbetiae_contig824:29686..297571622816907.3686538-CDS-L-elsbetiae_contig824:29686..29757Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig824 29687..29757 +
1691683903.1976395-CDS-L-elsbetiae_contig824:29686..297571691683903.1976395-CDS-L-elsbetiae_contig824:29686..29757Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig824 29687..29757 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig824.17247.1

>prot_L-elsbetiae_contig824.17247.1 ID=prot_L-elsbetiae_contig824.17247.1|Name=mRNA_L-elsbetiae_contig824.17247.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=131bp
GGVLRPPRRGRAPPGVPGAAGRDGPAAGDAYRQGAVLGVGGAEGGEGGRP
LAGGDQWASVRYGAVSQHEGAVARYLHHTGCQGCRGQPVCGGSPVAPKSA
LPPSRLQGDVPARRSRHKPGRAVETVEARD*
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mRNA from alignment at L-elsbetiae_contig824:27573..29757+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig824.17247.1 ID=mRNA_L-elsbetiae_contig824.17247.1|Name=mRNA_L-elsbetiae_contig824.17247.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=2185bp|location=Sequence derived from alignment at L-elsbetiae_contig824:27573..29757+ (Laminarionema elsbetiae ELsaHSoW15)
GGCGGCGTTCTCCGCCCCCCCCGACGAGGACGAGCACCACCTGGTGTTCC TGGGGCTGCTGGGAGGGACGGGCCAGCCGCTGGGGATGCTTACCGCCAGG GCGCAGTTCTCGGTGTCGGGGGGGCGGAGGGCGGAGAGGGAGGTCGGCCC CTTGCAGGTGAGGCAACATCTGTCTAAGTTTCTTCTAAATGCAGTAGTAA ACTCTTTTGTGCTTTACTTTGAGATAAAACGCAACTGACTGTAACATCAA CCCCCCCCCCCCCCCCCCCCCGAAAAACAAAATGTTCGACGATTCTTTTT TTTCTGTTGTCCAGGCGGCGACCAATGGGCGAGTGTACGGTACGGTGCAG TATCCCAGCATGAAGGAGCTGTGGCCAGATATTTACACCACACCGGGTGT CAGGGCTGTCGAGGTATGTCTCTGGCTTGCTCGGTACCTGAATTATTGAG ACAGACGGCTGGAAAAACGTGGTTTCTGTAGTTTTGTTGCGCATTTAGGG TGGCAGGGCGATTTCGACCCCCGCAGGGGATCTACCGCGGTGCCTCGGTA AGTTCGGGGTGCTTCTAGACAGACGTCCCCGGGGATACGCGACTTATGCA TGTTTTGTACTTGGCTGTTTTGCAGCGCGCGCCGCGTCACAAAAACATCC TGACTGCTACCCGCGTGTTGCTGCAGAAACGCCACCAAGGCATCAAGCGC CGCCCTCTCGCTTGTAGCTCGGCGTGCTGAACCGAGGAACTGACGTGTGT CATACGGACTGGGCTTAAACATGGTTGGCCTTTGTTTCGGGGGCATTCAA TGTTGCACGTTTCGAGAGCGGCAGGCAGTTGGCGCCCTTCTTGCGATTTG AAAAAAAAAAACAGATCATACACGTCATACTGTTCGTAGCTGTACGTAGC TGTACGTTGACACGTCTCTCCAGAAGGCTGCTCCTCTCTCGCAGTCGTCT TGTCTTGGTTCGCAGCTCCTCCCTCAGAAACGCCTCCTGCAGCACAAGCC CTTGTTCGGCGCGAGAGCAGACCAGACCCCACGCCCTCTCTTAGTCTGCG TGCCCCTTGGCCGCCGCCGTTGACGCTCGCTTAAGCACAGCAGCAAGCAT ACCCCCCCCCCCCCCTACAAAGCTCTCGCCTTCGTCGCCCCCAACCGCTC CCCTTGGACGCCCCCACCTGCCTCTGGCCACGCCGCTCGATATTTTCTGT GTTTTTTTCCGTCGGTACATCATACGCCGCGAAGGTCAACCTGTGTGTGG AGGTTCGCCCGTTGCACCGAAAAGTGCGCTACCTCCAAGTCGACTTCAAG GTGAGCGCCCCTCTCACGCCGTTGCCGTTGTCGTTGTCGTTGCGGTCGAC GTCGACGTCACCGTGCGTCCTATCTCTCGAGAAGGACAGGGCGGAGGGTT TCCGCGTGTCTGTGTTTGTCCGGGAAGGCTATGTAGAGTTGCGCCTCGTG TTTGGGGATTTGCACTAGCAGAGTAGGAACCTCTAGAACAGTACTTGTAG TTGTGTAGTATTCTTCACGGAAAGAAGTCGTCGACGAGCGTGTTTTTCCT CAGCAGCTAACAAACCTTTTTTGGCGGTCTTTTGCACCACCGTGCATAAA CAGCAACGTTAACGCTCATCCTGCCGCCAATGTCGGCGCGCGAGAACGAA GACCCCATCACACCGCCCAGCCCCCCCTTCACGCGAAACTATAGACGCCC GCCTCCAATTCAGAGGCACATCATTTTTCAGTACACCCTTTTTAGGGTAA GTTATCCTGTGACCACGGGAATCGACCGCGTCGGTGTTGAATTAGTACGG ACTCATGGAGGTGTACTGGATGGAGAGATCGGAACGGTTTTTAGATGTTA GGACAACAACAACAACAACAACAACAACAACAAGCCACAAACCTCACCCG TACTTACGAGCTTTATCCAGGCATCCTTGGGGCCTATCCATTCCAATAGC GAGCCCCTTAGCCGTGCAGCAGCCCCCGCAACGGCGACAACAACAACGGC CCTACAACTACGTTGGTGTTGTGTCTGACGTTCCTCGGACGAAGGAGTGT GTGTTCACCCTCGTTGTTGCCGTGCCCCCGTTCGTGCCGGACTCTGACTG TCTGCCCCGAGCAGGTGATGTACCGGCGAGGAGGAGTAGGCACAAGCCAG GGCGCGCTGTGGAGACCGTAGAGGCAAGGGACTAA
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Coding sequence (CDS) from alignment at L-elsbetiae_contig824:27573..29757+

>mRNA_L-elsbetiae_contig824.17247.1 ID=mRNA_L-elsbetiae_contig824.17247.1|Name=mRNA_L-elsbetiae_contig824.17247.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=786bp|location=Sequence derived from alignment at L-elsbetiae_contig824:27573..29757+ (Laminarionema elsbetiae ELsaHSoW15)
GGCGGCGTTCTCCGCCCCCCCCGACGAGGACGAGCACCACCTGGTGTTCC
TGGGGCTGCTGGGAGGGACGGGCCAGCCGCTGGGGATGCTTACCGCCAGG
GCGCAGTTCTCGGTGTCGGGGGGGCGGAGGGCGGAGAGGGAGGTCGGCCC
CTTGCAGGGCGGCGTTCTCCGCCCCCCCCGACGAGGACGAGCACCACCTG
GTGTTCCTGGGGCTGCTGGGAGGGACGGGCCAGCCGCTGGGGATGCTTAC
CGCCAGGGCGCAGTTCTCGGTGTCGGGGGGGCGGAGGGCGGAGAGGGAGG
TCGGCCCCTTGCAGGCGGCGACCAATGGGCGAGTGTACGGTACGGTGCAG
TATCCCAGCATGAAGGAGCTGTGGCCAGATATTTACACCACACCGGGTGT
CAGGGCTGTCGAGGCGGCGACCAATGGGCGAGTGTACGGTACGGTGCAGT
ATCCCAGCATGAAGGAGCTGTGGCCAGATATTTACACCACACCGGGTGTC
AGGGCTGTCGAGGTCAACCTGTGTGTGGAGGTTCGCCCGTTGCACCGAAA
AGTGCGCTACCTCCAAGTCGACTTCAAGGTCAACCTGTGTGTGGAGGTTC
GCCCGTTGCACCGAAAAGTGCGCTACCTCCAAGTCGACTTCAAGGTGATG
TACCGGCGAGGAGGAGTAGGCACAAGCCAGGGCGCGCTGTGGAGACCGTA
GAGGCAAGGGACTAAGTGATGTACCGGCGAGGAGGAGTAGGCACAAGCCA
GGGCGCGCTGTGGAGACCGTAGAGGCAAGGGACTAA
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