mRNA_L-elsbetiae_contig5295.13439.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig5295.13439.1
Unique NamemRNA_L-elsbetiae_contig5295.13439.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
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BLAST of mRNA_L-elsbetiae_contig5295.13439.1 vs. uniprot
Match: D7FWU3_ECTSI (Copper transport protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FWU3_ECTSI)

HSP 1 Score: 185 bits (469), Expect = 1.270e-53
Identity = 100/140 (71.43%), Postives = 117/140 (83.57%), Query Frame = 1 
Query:    4 AAQASTQCVLFLFEGAAVDSETKYAFAVIGAFLMGFTNEMIRYGRDRMAKTLDVSVASDLQRTAGFAVQIFVANMLMLLVMLYEYVILIFLILGLALGHLVTLRLTAAQRQGGT-NAGSKTPPEAVGSSGSPCCNTDTCA 420
            A  AS  C+LFLFEGA VD++TKYAFA +GAF MGF NEMIRYGRDRMAKT DVS+ASDL+ TA FAVQI++A MLMLLVMLYEYVILI +I GLA GHL+TLR +A +R+    +AG+KTPPE +GSSG+PCCNTDT A
Sbjct:   41 ATSASVNCILFLFEGAGVDTKTKYAFAALGAFFMGFANEMIRYGRDRMAKTSDVSLASDLKMTAAFAVQIYLAYMLMLLVMLYEYVILIMIISGLATGHLLTLRWSAGRRRAALRDAGAKTPPEVMGSSGTPCCNTDTVA 180
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BLAST of mRNA_L-elsbetiae_contig5295.13439.1 vs. uniprot
Match: D7FH71_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FH71_ECTSI)

HSP 1 Score: 163 bits (412), Expect = 8.390e-45
Identity = 90/139 (64.75%), Postives = 106/139 (76.26%), Query Frame = 1 
Query:    4 AAQASTQCVLFLFEGAAVDSETKYAFAVIGAFLMGFTNEMIRYGRDRMAKTLDVSVASDLQRTAGFAVQIFVANMLMLLVMLYEYVILIFLILGLALGHLVTLRLTAAQRQGGTNAGSKTPPEAVGSSGSPCCNTDTCA 420
            A  AS +C+LFLFEGA VD++TKYAFA +GAF MGF NEMIRYGRDRMAK   V V SD++ T  FAVQ+F+A MLMLLVMLYEYVILI +I GLA GHLVTLRL+A +R+     G+K      GSSG+PCCN+ T A
Sbjct:   68 ATSASVRCILFLFEGAGVDTKTKYAFAALGAFCMGFANEMIRYGRDRMAKASGVCVGSDVKATFAFAVQMFLAYMLMLLVMLYEYVILIMIISGLAAGHLVTLRLSAGRRRAALKDGAKEASGVAGSSGTPCCNSATQA 206
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BLAST of mRNA_L-elsbetiae_contig5295.13439.1 vs. uniprot
Match: W7TTP6_9STRA (Copper transport protein n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7TTP6_9STRA)

HSP 1 Score: 61.6 bits (148), Expect = 2.130e-7
Identity = 52/145 (35.86%), Postives = 71/145 (48.97%), Query Frame = 1 
Query:   25 CVLFLFEGAAVDSETKYAFAVIGAFLMGFTNEMIRYGRDRM--------------------AKTLDVSVASDLQRTAGFAVQIFVANMLMLLVMLYEYVILIFLILGLALGHLVTLRLTAAQRQGGTNAGSKTPPEAVGSSGSPC 399
            C+L+LF+GA V++  KYA A+IG F + F+ E +R+ R  M                       L   V         F V +FVA ++MLLVMLYE+ I I L+LGLA G+  T  +  A R     A  K  P A+G+ G  C
Sbjct:   33 CILYLFQGAVVNTAGKYAGALIGTFFLAFSVEALRHFRLWMRLRRATVVLAHGEKEGEVVTGPPLRAQVLPIFLEALLFGVHMFVAYLIMLLVMLYEWAIFISLLLGLAAGYF-TFEMWEAYRV----ANKKQLPSAIGAIGGGC 172
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BLAST of mRNA_L-elsbetiae_contig5295.13439.1 vs. uniprot
Match: A0A835YQY3_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YQY3_9STRA)

HSP 1 Score: 60.8 bits (146), Expect = 3.610e-7
Identity = 41/92 (44.57%), Postives = 53/92 (57.61%), Query Frame = 1 
Query:   25 CVLFLFEGAAVDSETKYAFAVIGAFLMGFTNEMIRYGRDRMAKTLDVSVASDLQRTAGFAVQIFVANMLMLLVMLYEYVILIFLILGLALGH 300
            CV++LF GA +D+E KYA AVIG FL+ F NE +R+ R RMA              A    Q+  A  LMLL MLYE V+   ++LGL  G+
Sbjct:   50 CVIWLFPGAILDTEGKYAAAVIGTFLLAFGNEGLRWLRTRMAAEQAPPXXXXXPSLALSVAQMVNAYWLMLLTMLYESVMFSMIVLGLMAGY 141
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BLAST of mRNA_L-elsbetiae_contig5295.13439.1 vs. uniprot
Match: W7U9L9_9STRA (Copper transport protein n=3 Tax=Monodopsidaceae TaxID=425072 RepID=W7U9L9_9STRA)

HSP 1 Score: 61.6 bits (148), Expect = 4.370e-7
Identity = 52/145 (35.86%), Postives = 71/145 (48.97%), Query Frame = 1 
Query:   25 CVLFLFEGAAVDSETKYAFAVIGAFLMGFTNEMIRYGRDRM--------------------AKTLDVSVASDLQRTAGFAVQIFVANMLMLLVMLYEYVILIFLILGLALGHLVTLRLTAAQRQGGTNAGSKTPPEAVGSSGSPC 399
            C+L+LF+GA V++  KYA A+IG F + F+ E +R+ R  M                       L   V         F V +FVA ++MLLVMLYE+ I I L+LGLA G+  T  +  A R     A  K  P A+G+ G  C
Sbjct:   91 CILYLFQGAVVNTAGKYAGALIGTFFLAFSVEALRHFRLWMRLRRATVVLAHGEKEGEVVTGPPLRAQVLPIFLEALLFGVHMFVAYLIMLLVMLYEWAIFISLLLGLAAGYF-TFEMWEAYRV----ANKKQLPSAIGAIGGGC 230
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BLAST of mRNA_L-elsbetiae_contig5295.13439.1 vs. uniprot
Match: A0A835YUT5_9STRA (Copper transport protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YUT5_9STRA)

HSP 1 Score: 58.9 bits (141), Expect = 2.090e-6
Identity = 52/173 (30.06%), Postives = 76/173 (43.93%), Query Frame = 1 
Query:   25 CVLFLFEGAAVDSETKYAFAVIGAFLMGFTNEMIRYGRDRMAK---------TLDV-SVASDLQRTAGFAVQIFVANMLMLLVMLYEYVILIFLILGLALGHLVTLRLTAAQRQGGTNA----------GSKTPPEAVG------------------------SSGSPCCNTD 411
            C+++LFEGA +D+  KY  AVIG FL+ F NE IR+ R R              D+ + A D+     +  Q+ +A  LML+VM YE  +   ++LGL  G+L    +    ++  T+           GS TPP+                           SS +PCCN D
Sbjct:   44 CIIWLFEGAVLDTAGKYTGAVIGTFLLAFANEGIRWLRSRAMSGKAPFTRCHLSDMPAFARDVVLALMYGFQMLIAYWLMLIVMTYEVFLFTAILLGLTAGYLTFNYVDRRAKEAATDCNCAVSAAKTVGSNTPPQLASEGSQNNLTEALAPMPETATAAWKVSSSTPCCNGD 216
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig5295.13439.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 6
Match NameE-valueIdentityDescription
D7FWU3_ECTSI1.270e-5371.43Copper transport protein n=2 Tax=Ectocarpus TaxID=... [more]
D7FH71_ECTSI8.390e-4564.75Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
W7TTP6_9STRA2.130e-735.86Copper transport protein n=1 Tax=Nannochloropsis g... [more]
A0A835YQY3_9STRA3.610e-744.57Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
W7U9L9_9STRA4.370e-735.86Copper transport protein n=3 Tax=Monodopsidaceae T... [more]
A0A835YUT5_9STRA2.090e-630.06Copper transport protein n=1 Tax=Tribonema minus T... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig5295contigL-elsbetiae_contig5295:7898..11528 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score139.0
Seed ortholog evalue1.3e-30
Seed eggNOG ortholog2880.D7FWU3
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Ctr copper transporter family
EggNOG OGs2E44A@1,2SB2P@2759
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Ec32 ortholog descriptionCtr copper transporter
Ec32 orthologEc-13_001170.1
Exons3
Model size1191
Cds size321
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig5295.13439.1prot_L-elsbetiae_contig5295.13439.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig5295 8666..11426 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816547.2091284-UTR-L-elsbetiae_contig5295:7897..86651622816547.2091284-UTR-L-elsbetiae_contig5295:7897..8665Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig5295 7898..8665 -
1691683638.3510473-UTR-L-elsbetiae_contig5295:7897..86651691683638.3510473-UTR-L-elsbetiae_contig5295:7897..8665Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig5295 7898..8665 -
1622816547.2614093-UTR-L-elsbetiae_contig5295:11426..115281622816547.2614093-UTR-L-elsbetiae_contig5295:11426..11528Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig5295 11427..11528 -
1691683638.4045737-UTR-L-elsbetiae_contig5295:11426..115281691683638.4045737-UTR-L-elsbetiae_contig5295:11426..11528Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig5295 11427..11528 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816547.2249076-CDS-L-elsbetiae_contig5295:8665..88541622816547.2249076-CDS-L-elsbetiae_contig5295:8665..8854Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5295 8666..8854 -
1691683638.3634303-CDS-L-elsbetiae_contig5295:8665..88541691683638.3634303-CDS-L-elsbetiae_contig5295:8665..8854Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5295 8666..8854 -
1622816547.2380211-CDS-L-elsbetiae_contig5295:10044..101501622816547.2380211-CDS-L-elsbetiae_contig5295:10044..10150Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5295 10045..10150 -
1691683638.3770792-CDS-L-elsbetiae_contig5295:10044..101501691683638.3770792-CDS-L-elsbetiae_contig5295:10044..10150Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5295 10045..10150 -
1622816547.2481213-CDS-L-elsbetiae_contig5295:11400..114261622816547.2481213-CDS-L-elsbetiae_contig5295:11400..11426Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5295 11401..11426 -
1691683638.3929157-CDS-L-elsbetiae_contig5295:11400..114261691683638.3929157-CDS-L-elsbetiae_contig5295:11400..11426Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5295 11401..11426 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig5295.13439.1

>prot_L-elsbetiae_contig5295.13439.1 ID=prot_L-elsbetiae_contig5295.13439.1|Name=mRNA_L-elsbetiae_contig5295.13439.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=107bp
MGFTNEMIRYGRDRMAKTLDVSVASDLQRTAGFAVQIFVANMLMLLVMLY
EYVILIFLILGLALGHLVTLRLTAAQRQGGTNAGSKTPPEAVGSSGSPCC
NTDTCA*
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mRNA from alignment at L-elsbetiae_contig5295:7898..11528-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig5295.13439.1 ID=mRNA_L-elsbetiae_contig5295.13439.1|Name=mRNA_L-elsbetiae_contig5295.13439.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=3631bp|location=Sequence derived from alignment at L-elsbetiae_contig5295:7898..11528- (Laminarionema elsbetiae ELsaHSoW15)
TTTGCGGCGCAGGCGAGCACGCAGTGCGTCCTCTTCCTGTTCGAAGGCGC
CGCCGTCGACTCCGAGACCAAGTACGCCTTCGCGGTCATCGGCGCCTTCC
TCATGGGCTTCACCAACGAGATGATCCGGTGAGGAGAGGAGAAGTGTGGT
GCTTTATATATATATATATTTCATGTTCTACGCTTGCTTGCTTGCTTTCG
TATTGTTGCGCTTTTGATCTTGCGGCGGGGCACGCCAAGCCCCTCGTGAC
GTCACCACCGGTCCGTTCAGCGGGAATCCCCGCGTGACGTCGCCACCGGC
TCGTTTAGCGGGAATCCCCTCGTGACGCCACCACCTGTTCGTTTAGCGGG
AGTCCCTTCGTGATGTCATATGTTTCCCACCTCCCGCGGTTCCCGTGTTT
GTTTCAGAAGGCAGTTGGTTGGCAAGTACGGCGGTATGTACGTGCCTCCC
TACCCCCCCCCCCCCTCTCGTTTCGCGAAATTTTCCCAGTTCTACATACA
ATACAAGATCACACTAAATTAAGGGCAACCTCCACGTCTCCCCAGTCCAG
CTCTGTGTTGAACGCCTCTCGAATCTCGCTGGATCGTGCGGACCCTGTCC
CACCAGACACAAAATTGTCGTTTTTTTATGTTGTCGGGGCTGATTTTTCA
ATTCTACATACACAGCAGTATATACTTTTTCCTTTTTCCCTCTTGCTGTA
TCAATCATGTGCGTGTGCGGTTATACAATAATGGGCGGGGAGGACTAGAT
TGGTGGGCCTGACCGGCGAGAACCCGGCGCGGAACACCCCGGCGTCGTTC
GAGAACCAAGATAGAACCAGGGGGTGTAAACACCCCGCGGTTGTGTGCGA
TGTTGGTTTTCCCACGTTTTTTTCACCTCACATGGCGTGTAGAGGGGGAG
CGGGGGGGGGGCTCCGGACTATCTGTGATATTTTGTTTTTTTGTGCTTTG
TGAGCCCTTTGGGTCCATCTCATTTCTCTTTCTTGTTTTCTCAGTAGTCC
ATTCCCTTTCTTAGTTTATCCTGTGACCACGGGAATCGACCGCGTCAGTG
TTGGATTAGTACGCGGACTTATGGAGGTGTACTGGATAGAGAGATCGGAG
TGGTTTTTAATGTGAAGACAACCACATCATACATCGTGCCCTGTTCTTGG
GCCGTTGATGGGCGATCCCAGGGAATCGTTTTGCTCCGTTTTGGCGCTCG
CGCTCTGCTTGCTGTGTGTGTGTGTCCACGCGGTGGTCTCGGCAAGCAAG
AATTTTCTGTCTTAAGTACGTTTAAGGCAATTTCTGACAACAAGTGCCCC
GTTATCTGGCTTGTTTTTGTTTTGTTCTCGTGCTCCGTCCTCTGCTCGCG
CCTCGCAATTCCCGTTCCCTTTAAATAGCTACGGGCGCGATCGAATGGCC
AAGACCTTGGATGTATCCGTCGCGTCGGACCTCCAGAGGACTGCCGGCTT
CGCCGTCCAGATATTCGTGGCCAACATGCTCATGGTAAGCTCTGGCCCTG
ATTTGTGTCTCATCCCCCCCTTCACGCTGTAAGTCCTCAGCATGGAAATG
TAGACCCCTTCTGCAATAGAGCCAGCCCCCAATTCTCAAAAAGAGCTGTG
GGGGCAGCGGGGGAGGGGGGGGGGGGGTATTTATCTAATAAATAGAGTTA
AAAAATTCCCAAAGCCACTTTTCAGAATTGGGAGCCTTGATTGGGGGAAG
CCTATTGGTCCGGGCAGATGTTGCACGCGTCTCTTTTCGAGCTCTTGCCG
CCGGTGGATGTGGTTGCCTCTTGGTTTCTTCGGCTGAGAAGACTTGGTTT
CTTCGGCTGAGAAGACTCTTGGTTTCTTCGGCTGAGAAGACCCCCGCGCG
GCAGAGACCCTCGAGTGCACGGCGGCTTTTGCCGTGTTTCTGACTGAGAT
GTTCTAGGGTTTGTCTTTATATTTTTGCGTGCGAAGCTGTCGTGGTTTTG
GTCTAATTATTTAGTGTACGGTTGTGCGCGCGGCTCACGCCAAGAGAAGG
AGGACGTATTTTTTCAGGCGGGTTTGGTCAAACAGAAGAACGGCTGAACC
CTTCTCGCGACGAGAATCTGAGGCAAGCACCACCGCAGACGGGGGGAGTG
GGGACCTTGGGGAAAGGAACCCTTCTGTGTGACCAACCACGGTTCAGTAC
TCTGGCAAGATGTCCTCGTGACAGGGAGCTCATCGAGAACTAGACATATA
GTCTATGTGAAGAAACAATAAAGGGAACATGACGTAGGTACGCACTCGTG
TTGAATGTACTTTGTTTTTGGGCGTCCGACCTTTCTTTTGTCCCCAAGCG
CCTGGAGCATACGGTACATATGATAGGCACAGATGATCAGTGCAGAGGGG
GGTTGCCGCTGTTGTCAAGCACTGCAGACGGAGGCCGCCTGTGTTTAGTG
CGCGCGTGCTCCTGTACCTCGCCTGTACGCACCGTACTCGAATCAACCAG
CCTACAAAATGGTACTATTATTGTACTGCTGCTGTGTGTGCTTGGGTTAG
AGCTCGACATTGCTGCCGGCCCACAAGCTTGTCTCTCACAGGTGTGCACG
TGGTGCCCCTGAACATTGGGAGTCATTGTGATCGATCAAACACCGCGAGC
GCTGACCCCCTCGTGACCAATGGATGCTTACCGATACCCCCTCCCCCCCC
CCTTCGATTTCCCGTTCCCCCCAGCTCTTGGTGATGCTCTACGAGTATGT
GATCCTCATCTTCCTCATCCTGGGCCTCGCTTTGGGGCACCTGGTCACCC
TCCGACTCACGGCGGCCCAGCGACAGGGGGGCACGAACGCCGGCTCGAAG
ACACCTCCGGAGGCGGTGGGCAGCTCGGGCTCCCCGTGCTGCAACACCGA
CACATGCGCGTGAAACCACAATACACATCGGCGCGACAGTAGTGGTGTGC
GCTCTTACCGGGGGTACGACGGCTCAGAAATTCCGGGGCGGCACTCACGT
CGCCACGGCTTTGTATACATAACGGGGGGCTCCGCGCCGCTACCCTGTTT
GGAAACCGTTGGCAGGCAGTAGATGCTTCTTTTTTTTTTCATGATCCTCT
GCGCTCGTCGTGTGGTTTCGATATGTTTTCACGGTGCAGCGAGGATGTTT
TGTGTCGAGGTCTGTTGTTGTTTCATTATGCTGCGCTCGTCGTGTTGTGT
GGTCGACCATAGAGCCACACATATTGCTGTTTGGCGTAAAAAATGCTCCC
GTCGCAACCGGCGAGTATAAACGCGTGTTTCTGTTGTAAAAGGGTGCTGT
TGTTGTGCGGATCGACCATTGTTGTTGTTGGGCTCGACAAGCGGCGCGTT
TTCATTATGTTTTATTGTTTTGTGTATGTACTACTACTCTGTTGTGGGTA
AGTTATGGGCGAGGTATACTCGACCTTTCGAGGAGCTTGACTCTTCGCAC
TTAAAATATGCCTGACTCGGTTTTGTGTTGAGCTCTGGTAGGCTCTCCCA
GTATGTCAGGAAATCCCAAGTATTCACCCATGTCTCGTATGTCCTACAGC
AACACAGTAGTAGACGTAGTTTTCTCGAGGCATTCCAAATCAGCGGGGGA
AAAAGCGCGCGGTCTTGACATGTGGTACCTGTGTGTCTGCACATGCACAT
GTTGGTGGTGTGCAATACTCTCGAACCGGCG
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Coding sequence (CDS) from alignment at L-elsbetiae_contig5295:7898..11528-

>mRNA_L-elsbetiae_contig5295.13439.1 ID=mRNA_L-elsbetiae_contig5295.13439.1|Name=mRNA_L-elsbetiae_contig5295.13439.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=642bp|location=Sequence derived from alignment at L-elsbetiae_contig5295:7898..11528- (Laminarionema elsbetiae ELsaHSoW15)
ATGGGCTTCACCAACGAGATGATCCGATGGGCTTCACCAACGAGATGATC
CGCTACGGGCGCGATCGAATGGCCAAGACCTTGGATGTATCCGTCGCGTC
GGACCTCCAGAGGACTGCCGGCTTCGCCGTCCAGATATTCGTGGCCAACA
TGCTCATGCTACGGGCGCGATCGAATGGCCAAGACCTTGGATGTATCCGT
CGCGTCGGACCTCCAGAGGACTGCCGGCTTCGCCGTCCAGATATTCGTGG
CCAACATGCTCATGCTCTTGGTGATGCTCTACGAGTATGTGATCCTCATC
TTCCTCATCCTGGGCCTCGCTTTGGGGCACCTGGTCACCCTCCGACTCAC
GGCGGCCCAGCGACAGGGGGGCACGAACGCCGGCTCGAAGACACCTCCGG
AGGCGGTGGGCAGCTCGGGCTCCCCGTGCTGCAACACCGACACATGCGCG
TGACTCTTGGTGATGCTCTACGAGTATGTGATCCTCATCTTCCTCATCCT
GGGCCTCGCTTTGGGGCACCTGGTCACCCTCCGACTCACGGCGGCCCAGC
GACAGGGGGGCACGAACGCCGGCTCGAAGACACCTCCGGAGGCGGTGGGC
AGCTCGGGCTCCCCGTGCTGCAACACCGACACATGCGCGTGA
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