mRNA_L-elsbetiae_contig4835.12733.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig4835.12733.1
Unique NamemRNA_L-elsbetiae_contig4835.12733.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
BLAST of mRNA_L-elsbetiae_contig4835.12733.1 vs. uniprot
Match: D7G8M6_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G8M6_ECTSI)

HSP 1 Score: 122 bits (305), Expect = 1.070e-27
Identity = 76/155 (49.03%), Postives = 82/155 (52.90%), Query Frame = 1
Query:  226 LPSQQTRTVLSGSLSRCSPVFSASSDTVFCACSSIVLGVSVETGEELLRLVGHAGDVTAMLVLPPGAYGAADSGGAAASTSARHQRLMTSSLDGTLRLWDVSADLMSLEGEAERCLRVLNVGMPVYQLFMGRQDSSGAALDVIIVTKEGTSSLHG 690
            L S++TR VLSGSL RCSPVFS SSD VFCACSS VLGVSVETGE+                                                               GEAERCLRV NVGMPVY LFMGRQD SG ALDVI+VTKEGT++LHG
Sbjct:   30 LASEETRAVLSGSLFRCSPVFSPSSDAVFCACSSSVLGVSVETGEQ---------------------------------------------------------------GEAERCLRVYNVGMPVYHLFMGRQDGSGTALDVIVVTKEGTAALHG 121          
BLAST of mRNA_L-elsbetiae_contig4835.12733.1 vs. uniprot
Match: A0A6H5KD90_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KD90_9PHAE)

HSP 1 Score: 75.1 bits (183), Expect = 3.650e-11
Identity = 57/165 (34.55%), Postives = 73/165 (44.24%), Query Frame = 1
Query:  211 VALAALPSQQTRTVLSGSLSRCSPVFSASSDTVFCACSSIVLGVSVETGEELLRLVGHAGDVTAMLVLPPGAYGAADSGGAAASTSARHQRLMTSSLDGTLRLWDVSADLMSLEGEAERCLRVLNVGMPVYQLFMGRQDSSGAALDVIIVTKEGTSSLHGLQKDG 705
            VA   L S +TR VLSG L RCSPVFS SSDTVFCACSS VLGVSVETGE+ +                                                        + ++      C+  L+              ++G+A+DVI+VTKEGT+SLHG  ++G
Sbjct:   61 VAPPILASDETRAVLSGCLFRCSPVFSPSSDTVFCACSSSVLGVSVETGEQTI--------------------------------------------------------ISNILNAQSSCVHRLSPSPANNFPPRDNTGNNGSAMDVIVVTKEGTASLHGYGEEG 169          
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig4835.12733.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 2
Match NameE-valueIdentityDescription
D7G8M6_ECTSI1.070e-2749.03Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5KD90_9PHAE3.650e-1134.55Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig4835contigL-elsbetiae_contig4835:964..4681 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score124.8
Seed ortholog evalue7.4e-26
Seed eggNOG ortholog2880.D7G8M6
Preferred nameWDR75
KEGG koko:K14552
KEGG Pathwayko03008,map03008
Hectar predicted targeting categoryother localisation
GOsGO:0000462,GO:0000775,GO:0000776,GO:0000785,GO:0000792,GO:0003674,GO:0003676,GO:0003723,GO:0003729,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005694,GO:0005730,GO:0005737,GO:0005813,GO:0005815,GO:0005856,GO:0005874,GO:0005875,GO:0005938,GO:0006139,GO:0006355,GO:0006356,GO:0006364,GO:0006396,GO:0006725,GO:0006807,GO:0008017,GO:0008092,GO:0008150,GO:0008152,GO:0009889,GO:0009891,GO:0009893,GO:0009987,GO:0010467,GO:0010468,GO:0010556,GO:0010557,GO:0010604,GO:0010628,GO:0015630,GO:0015631,GO:0016070,GO:0016072,GO:0019219,GO:0019222,GO:0022613,GO:0030490,GO:0030515,GO:0030684,GO:0030686,GO:0031323,GO:0031325,GO:0031326,GO:0031328,GO:0031974,GO:0031981,GO:0032040,GO:0032991,GO:0033553,GO:0034455,GO:0034470,GO:0034511,GO:0034641,GO:0034660,GO:0042254,GO:0042274,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0044085,GO:0044087,GO:0044089,GO:0044237,GO:0044238,GO:0044422,GO:0044424,GO:0044427,GO:0044428,GO:0044430,GO:0044444,GO:0044446,GO:0044452,GO:0044464,GO:0044877,GO:0045893,GO:0045935,GO:0045943,GO:0046483,GO:0048518,GO:0048522,GO:0050789,GO:0050794,GO:0051171,GO:0051173,GO:0051252,GO:0051254,GO:0060255,GO:0065007,GO:0070013,GO:0070840,GO:0071704,GO:0071840,GO:0071944,GO:0080090,GO:0090069,GO:0090070,GO:0090304,GO:0097159,GO:0098687,GO:0099080,GO:0099081,GO:0099512,GO:0099513,GO:0099568,GO:1901360,GO:1901363,GO:1902680,GO:1903506,GO:1903508,GO:1990904,GO:2000112,GO:2000232,GO:2000234,GO:2001141
EggNOG free text desc.rRNA processing
EggNOG OGsKOG1963@1,KOG1963@2759
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko03009
Exons5
Model size979
Cds size975
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig4835.12733.1prot_L-elsbetiae_contig4835.12733.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig4835 966..4681 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816474.9377508-CDS-L-elsbetiae_contig4835:965..13021622816474.9377508-CDS-L-elsbetiae_contig4835:965..1302Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig4835 966..1302 -
1691683586.6233227-CDS-L-elsbetiae_contig4835:965..13021691683586.6233227-CDS-L-elsbetiae_contig4835:965..1302Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig4835 966..1302 -
1622816474.949023-CDS-L-elsbetiae_contig4835:2039..21251622816474.949023-CDS-L-elsbetiae_contig4835:2039..2125Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig4835 2040..2125 -
1691683586.6431556-CDS-L-elsbetiae_contig4835:2039..21251691683586.6431556-CDS-L-elsbetiae_contig4835:2039..2125Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig4835 2040..2125 -
1622816474.9612894-CDS-L-elsbetiae_contig4835:2824..30131622816474.9612894-CDS-L-elsbetiae_contig4835:2824..3013Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig4835 2825..3013 -
1691683586.6547039-CDS-L-elsbetiae_contig4835:2824..30131691683586.6547039-CDS-L-elsbetiae_contig4835:2824..3013Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig4835 2825..3013 -
1622816474.9736378-CDS-L-elsbetiae_contig4835:3816..38831622816474.9736378-CDS-L-elsbetiae_contig4835:3816..3883Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig4835 3817..3883 -
1691683586.6655846-CDS-L-elsbetiae_contig4835:3816..38831691683586.6655846-CDS-L-elsbetiae_contig4835:3816..3883Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig4835 3817..3883 -
1622816474.9893887-CDS-L-elsbetiae_contig4835:4385..46811622816474.9893887-CDS-L-elsbetiae_contig4835:4385..4681Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig4835 4386..4681 -
1691683586.6755137-CDS-L-elsbetiae_contig4835:4385..46811691683586.6755137-CDS-L-elsbetiae_contig4835:4385..4681Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig4835 4386..4681 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig4835.12733.1

>prot_L-elsbetiae_contig4835.12733.1 ID=prot_L-elsbetiae_contig4835.12733.1|Name=mRNA_L-elsbetiae_contig4835.12733.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=325bp
ALHSSRQASAARGEMQPPAARSKREGRRSSVSSAGRSSSSASSSTNKNRR
ASSGGGQQLATVAGGGGAGAVALAALPSQQTRTVLSGSLSRCSPVFSASS
DTVFCACSSIVLGVSVETGEELLRLVGHAGDVTAMLVLPPGAYGAADSGG
AAASTSARHQRLMTSSLDGTLRLWDVSADLMSLEGEAERCLRVLNVGMPV
YQLFMGRQDSSGAALDVIIVTKEGTSSLHGLQKDGGGGENDEARQKKKTA
AGTVGAARSPNGVQGMAGGDDADLTSDDEEEEDDDSSSDDDNDDDVEVLD
GGGSGDDDDVVEIDAEGGTASTKGS
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mRNA from alignment at L-elsbetiae_contig4835:964..4681-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig4835.12733.1 ID=mRNA_L-elsbetiae_contig4835.12733.1|Name=mRNA_L-elsbetiae_contig4835.12733.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=3718bp|location=Sequence derived from alignment at L-elsbetiae_contig4835:964..4681- (Laminarionema elsbetiae ELsaHSoW15)
GCTCTCCACTCAAGCCGACAAGCATCAGCAGCAAGGGGGGAGATGCAGCC GCCTGCAGCGAGATCGAAGAGGGAGGGTCGTCGGTCCTCCGTTTCTTCTG CTGGCCGCAGCAGCAGCAGCGCCTCGAGCAGCACCAACAAAAACCGCCGC GCTAGCAGCGGTGGTGGACAGCAGCTCGCTACCGTTGCTGGCGGCGGCGG GGCTGGTGCTGTGGCCTTGGCCGCGCTCCCTTCGCAACAGACGAGGACGG TGCTCTCTGGGAGCCTTTCTCGCTGCTCTCCTGTCTTCAGCGCCTCGTAA GTTGGTCCTGGTGCTCCTAAGTTGGTAGAGATGCCGGGTTGGCTGGAGAG ATTTTGTAGCGTGGCAACAGCACGGGGTTGCTTGCTAACCAACCTGGTGT GCTTCGACTCGCGTGTTTTTAACTCCAGCAGTGGCCATTTCAAGGCGTCC ATCACGCACCAGCTCGGTGTAGATACCCGTCTGTTCGCTTTCGACGACCG GAAGCGTGGCTTTGGGGCACCTCGCCGCAAAGGCTTTCATCATGAGCCTT CGTATCTAACACAGAGATAAGAGTCTAGTCTAGATACTGGCAGCACGCGC GCACGCGAACCTTCGGCCCACAGCGTGTTGCACAACAGTGAACCACAACG CTCTATCCCTGCTGTCACCACGAACTCACTGTTTCCTGTACGATAGCCCT GCTTCCACTACTGCAACAGCTTTGAGTGGCAGGCCGTGCTGATGGCTGTT TTTGTAACCCCCCCGACCCGCTGCTCATCGACCAATGTCGTACCACAGGT CCGACACAGTTTTCTGTGCGTGCAGCTCGATTGTGCTTGGTGTCAGCGTC GAGACGGGCGAGGAGGTGCGCGCCTGTTTATTTTCATTCAGTTGGTAATT TACATCATCAATACATCTCACACAGGTATTTAGGTACTTATTTACAAGCG GCGTTGTTGTGCTCAAATCTAGATACCGGTAATCTTTTGTTCTCAGGAGG AAAGGTCTCTTGCTATGCCAGCGGTAGGTAGCTCGGGACCGGGACGCCAC ATGCAGCAGTGTATAGACTACATACATGTGTCCAACCTGTGAGGCAAGCC TTTCAGTGGGAGCTGTTGTAGACACATGTGTAACCCTGTATCGAGGAGGA AATATCGCTCTCTTGCCAGCGGTAGGTAGCTCCAGACCGGGACGCCACAT GCAGCAGTCTACCGTAGGTGACACAAGATACCACACCCCCTGGGCCGAAT TTTAGAGCTCCATTGCTCCGTAACGAAGAGGGTGCCAGCACCAAACGACA CATTTTCGGACAGCCCTGGACTAGATCTTTCCAACGCATCCTCTTTCGGC ACTGGCGCTGTTCTTGCTGGGGAGGAATCGAGCTTTGAAAATATGTCCAA GGGGTGTGGTATCTTGTGTCACCTACGGTAGTTTATGGGCATATATATGT CCAGCCTGTGAGGCAAGTCTTTCCAGTGGGAGCTGTTGTAGACACACTAA CCCTCCACCACCGCCCCCAGCAGCTATATACGGAGTGTACAAAAGGCACA GCAATCTCTCGCTTCCCAAGCCGACACCGCTTTGACCCCCTTGACCAACG ACAACACTCGACCCTGACGCCTGAACACCTCACCCCCCTTCCCCCTCCCT CTCGTCCACATGGCAAAGCTTCTGCGGCTGGTGGGGCACGCCGGGGATGT GACCGCCATGCTCGTGCTGCCACCCGGGGCGTACGGGGCCGCTGACAGCG GCGGCGCAGCTGCCTCCACTTCCGCTCGACACCAGCGCCTCATGACCTCC AGCCTGGACGGGACGCTGAGGCTGTGGGATGTCTCCGCCGACCTCATGTC TCTCGAGGTGGGCTGGGAGTTGCTCCAGTGTGTATCGGCCCGCCCTTTTG TGTCCACACTTTATGAAAAACGGAAATTTTAAGTTTGGGCCGAGGCTAGG TTTCTCATGCGAGGCCAAAGATGATCGCCGTCTCTTTAATTAATGTATAC AAATGTCAGCAGTCTACGTCCGGTTTGTGAAAATATCGTTCCAGAGGGAT CTGTTGTAGGCATACTAACCCCCCTTTTCCATCCCAAGATATACTCACAC AAAATCCGTAATCCATATAATATGCCATACATAGATACATATATACAAGT TCGTTGGTGAAAGTACTCTTTCAGAAGTCGGTTGCAGTGTAAACGTGAGC GCATCAAGCATTTGGCATGGTACAATGTATATGGTGTAGGTAGGTGCTAT CAGGTCATCATTTGTAGGAGGCTGTACACTAATGATTTTGGGTTACAGTA GTAGTAGCGGCGTGAGGGATTAGTTGTTCATAGTGATGTTCTCGTCACGA AGCCCCTCCCCCCCTTCCGCCCCCCCCCGAAATCTCAGTCTGCTGTTTGC CTTGTCGTAAAAAATTACATGTTATTTCTACGAAAAGGAATACTCCGGAT TAGGTCCCAGCCTCGGAAATGAAGGTGGAAGGAAGCCCGCATCAGACGTT TTTTTTTTTCACCATCACAATGCTCGCTCTCTTTTTCTCTCTATTCGCGC GCGCAGGGAGAGGCGGAGAGGTGTCTCCGTGTCTTGAACGTGGGGATGCC GGTGTACCAGCTCTTCATGGGAAGACAAGACAGCAGCGGGGCGTGAGTGC CGTGTGTGTTGTCTGCTCTCTCCTGGTGTTGGTGTTGTTGTTGTTGTTGT TTTATCTTTTTCCGTATGTTGCTGCCGTGGTCGTACTTTCACGAAGCTTT GCGCTCTACAAGGTACCTACAGCAGTGTAACGGGGTAGCAGGCCTTTATT GTCGTGTGCACGTGGCGCCCTATTCGACGGGTTGTTGGCTTTTGCGGCTT TTCTTTTCGTCTGGAAAATCGTCGTCTGCGGTTGTAGTCCATATATTACT CATGATAAACTTGTGAATAGCATCTTCGGGCTACGGAATGCTTTGTGAAT ATATTCAAAGGATATATTTGTCACGGAATGCTTGCTGATGGAGCAAGTTT ACTGTTGCACGGCGCCCGAGAGGCGCGATTCCCGCTAGAGCGAGCTGCCG GCAGGGCAGAAGGCAGCACCACACGCCACGGGGAACGGTCATAGTGGCGG GCCGGTGATAATCGTACACGCATACCTGTCGCACAATAGAATCAGCGTGC AGTCCTTTCGGCCTTTTGGTACGAAAAGAGTGTTTTTTTTGTTTTTGTTT CCTGTGTCGTGTTTTCCCTCCCGGTGACGCGCGCCCGCCTGGCTTCCTCG ATCCCGCTTTTCGCTTGAAACAGCCAACCACCCACACGCTCTCTTTTCGT CTGCCTCCCGCCCGCGATTGCACTGCTGCTGCTGCTGCTGCTGCTGTGTG CGTGTTCACTATTGTCTGATGGTGAGCAGAGCCCTGGACGTGATCATCGT CACCAAGGAGGGAACCTCGTCTCTGCACGGGCTGCAGAAGGACGGCGGCG GCGGTGAGAACGACGAGGCGAGACAGAAGAAGAAGACGGCGGCGGGGACG GTGGGGGCCGCGAGGTCCCCAAACGGCGTCCAAGGCATGGCTGGGGGCGA TGACGCCGACCTTACCTCGGACGACGAGGAGGAGGAGGACGACGATTCTT CTTCTGACGACGACAACGACGACGATGTCGAAGTCCTCGACGGAGGCGGC AGCGGTGACGACGACGATGTTGTTGAGATCGATGCCGAGGGGGGCACTGC CTCGACAAAGGGCTCGGG
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Coding sequence (CDS) from alignment at L-elsbetiae_contig4835:964..4681-

>mRNA_L-elsbetiae_contig4835.12733.1 ID=mRNA_L-elsbetiae_contig4835.12733.1|Name=mRNA_L-elsbetiae_contig4835.12733.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=1950bp|location=Sequence derived from alignment at L-elsbetiae_contig4835:964..4681- (Laminarionema elsbetiae ELsaHSoW15)
GCTCTCCACTCAAGCCGACAAGCATCAGCAGCAAGGGGGGAGATGCAGCC
GCCTGCAGCGAGATCGAAGAGGGAGGGTCGTCGGTCCTCCGTTTCTTCTG
CTGGCCGCAGCAGCAGCAGCGCCTCGAGCAGCACCAACAAAAACCGCCGC
GCTAGCAGCGGTGGTGGACAGCAGCTCGCTACCGTTGCTGGCGGCGGCGG
GGCTGGTGCTGTGGCCTTGGCCGCGCTCCCTTCGCAACAGACGAGGACGG
TGCTCTCTGGGAGCCTTTCTCGCTGCTCTCCTGTCTTCAGCGCCTCGCTC
TCCACTCAAGCCGACAAGCATCAGCAGCAAGGGGGGAGATGCAGCCGCCT
GCAGCGAGATCGAAGAGGGAGGGTCGTCGGTCCTCCGTTTCTTCTGCTGG
CCGCAGCAGCAGCAGCGCCTCGAGCAGCACCAACAAAAACCGCCGCGCTA
GCAGCGGTGGTGGACAGCAGCTCGCTACCGTTGCTGGCGGCGGCGGGGCT
GGTGCTGTGGCCTTGGCCGCGCTCCCTTCGCAACAGACGAGGACGGTGCT
CTCTGGGAGCCTTTCTCGCTGCTCTCCTGTCTTCAGCGCCTCGTCCGACA
CAGTTTTCTGTGCGTGCAGCTCGATTGTGCTTGGTGTCAGCGTCGAGACG
GGCGAGGAGGTCCGACACAGTTTTCTGTGCGTGCAGCTCGATTGTGCTTG
GTGTCAGCGTCGAGACGGGCGAGGAGCTTCTGCGGCTGGTGGGGCACGCC
GGGGATGTGACCGCCATGCTCGTGCTGCCACCCGGGGCGTACGGGGCCGC
TGACAGCGGCGGCGCAGCTGCCTCCACTTCCGCTCGACACCAGCGCCTCA
TGACCTCCAGCCTGGACGGGACGCTGAGGCTGTGGGATGTCTCCGCCGAC
CTCATGTCTCTCGAGCTTCTGCGGCTGGTGGGGCACGCCGGGGATGTGAC
CGCCATGCTCGTGCTGCCACCCGGGGCGTACGGGGCCGCTGACAGCGGCG
GCGCAGCTGCCTCCACTTCCGCTCGACACCAGCGCCTCATGACCTCCAGC
CTGGACGGGACGCTGAGGCTGTGGGATGTCTCCGCCGACCTCATGTCTCT
CGAGGGAGAGGCGGAGAGGTGTCTCCGTGTCTTGAACGTGGGGATGCCGG
TGTACCAGCTCTTCATGGGAAGACAAGACAGCAGCGGGGCGGAGAGGCGG
AGAGGTGTCTCCGTGTCTTGAACGTGGGGATGCCGGTGTACCAGCTCTTC
ATGGGAAGACAAGACAGCAGCGGGGCAGCCCTGGACGTGATCATCGTCAC
CAAGGAGGGAACCTCGTCTCTGCACGGGCTGCAGAAGGACGGCGGCGGCG
GTGAGAACGACGAGGCGAGACAGAAGAAGAAGACGGCGGCGGGGACGGTG
GGGGCCGCGAGGTCCCCAAACGGCGTCCAAGGCATGGCTGGGGGCGATGA
CGCCGACCTTACCTCGGACGACGAGGAGGAGGAGGACGACGATTCTTCTT
CTGACGACGACAACGACGACGATGTCGAAGTCCTCGACGGAGGCGGCAGC
GGTGACGACGACGATGTTGTTGAGATCGATGCCGAGGGGGGCACTGCCTC
GACAAAGGGCTCGAGCCCTGGACGTGATCATCGTCACCAAGGAGGGAACC
TCGTCTCTGCACGGGCTGCAGAAGGACGGCGGCGGCGGTGAGAACGACGA
GGCGAGACAGAAGAAGAAGACGGCGGCGGGGACGGTGGGGGCCGCGAGGT
CCCCAAACGGCGTCCAAGGCATGGCTGGGGGCGATGACGCCGACCTTACC
TCGGACGACGAGGAGGAGGAGGACGACGATTCTTCTTCTGACGACGACAA
CGACGACGATGTCGAAGTCCTCGACGGAGGCGGCAGCGGTGACGACGACG
ATGTTGTTGAGATCGATGCCGAGGGGGGCACTGCCTCGACAAAGGGCTCG
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