mRNA_L-elsbetiae_contig2034.6117.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig2034.6117.1
Unique NamemRNA_L-elsbetiae_contig2034.6117.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
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BLAST of mRNA_L-elsbetiae_contig2034.6117.1 vs. uniprot
Match: D7FPJ4_ECTSI (Vacuolar sorting receptor, putative (ISS) n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FPJ4_ECTSI)

HSP 1 Score: 163 bits (412), Expect = 1.170e-48
Identity = 79/104 (75.96%), Postives = 89/104 (85.58%), Query Frame = 1 
Query:  295 MAASIVLFERGEVPVVEKVLKAQAAGAAGIVVVDDGGCSDGLVDCGRLGGVRDGGFAGRDGAHAWLTVKIPVVLISAAQGERLREMMPLQKVAVKGLGEQLVER 606
            MAA +VLFERGEVPVVEK+LKAQ AGA G++VVD+GGC DGLVDCGRLGG RDGGFA RDG HAW  VKIP V++SAA GER R MM LQK+ V+GLGEQLV+R
Sbjct:    1 MAACVVLFERGEVPVVEKILKAQTAGAVGVIVVDNGGCDDGLVDCGRLGGARDGGFAKRDGVHAWSGVKIPAVMVSAADGERFRGMMLLQKIVVEGLGEQLVQR 104
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BLAST of mRNA_L-elsbetiae_contig2034.6117.1 vs. uniprot
Match: A0A835Z4S0_9STRA (PA domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z4S0_9STRA)

HSP 1 Score: 161 bits (407), Expect = 1.280e-46
Identity = 80/159 (50.31%), Postives = 110/159 (69.18%), Query Frame = 1 
Query:  124 PLLHLHFCLGCLDAAAEGSLVQALAPHHGIRDFKVSGDLVYAVPNKGNADVLLNGDDMAASIVLFERGEVPVVEKVLKAQAAGAAGIVVVDDGGCSDGLVDCGRLGGVRDGGFAGRDGAHAWLTVKIPVVLISAAQGERLREMMPLQKVAVKGLGEQLV 600
            PL ++ FCL C D   EGS V AL   HG R F  +GD++YAVPN G+A+ LLN D++A  I L +RG +P++EKVLK QAAGA G ++VD+G CS+  + CGR GGV +GGFA RD  + W  VKIP +L+S  +G R++ +M L+ + + G G+QLV
Sbjct:   40 PLQYVEFCLDCPDPHLEGSHVPALRAAHGQRAFNATGDIIYAVPNDGSAE-LLNPDEVAGHIALLDRGTIPLIEKVLKVQAAGAVGALLVDNGECSEDFMRCGRTGGVPEGGFAWRDQPYDWSKVKIPALLVSEKEGSRVKALMSLRSIFITGHGDQLV 197
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BLAST of mRNA_L-elsbetiae_contig2034.6117.1 vs. uniprot
Match: A0A482SJ43_9ARCH (PA domain-containing protein n=1 Tax=archaeon TaxID=1906665 RepID=A0A482SJ43_9ARCH)

HSP 1 Score: 129 bits (324), Expect = 6.400e-34
Identity = 63/160 (39.38%), Postives = 99/160 (61.88%), Query Frame = 1 
Query:  112 DAPFPLLHLHFCLGCLDAAAEGSLVQALAPHHGIRDFKVSGDLVYAVPNKGNADVLLNGDDMAASIVLFERGEVPVVEKVLKAQAAGAAGIVVVDDGGCSDGLVDCG-RLGGVRDGGFAGRDGAHAWLTVKIPVVLISAAQGERLREMMPLQKVAVKGLG 588
            DA   L  + +CL C D  +EGS++ +L   +G+ ++ V+G+LVY VPN      L N   ++  I   +RGE+ +++KVLK Q A A G+V+ DDG C +    CG R G +++GGFA  D   +WL V+IPVV++++   +RLR+ M ++ V + GLG
Sbjct:   34 DADGALKWITYCLKCPDPRSEGSILPSLNAKYGVDEYSVTGELVYCVPNYAEGPFLANDHQLSDRIAFVDRGEISLLDKVLKIQEANALGVVIADDGSCYEDFHYCGPRAGSLQEGGFAAYDNEQSWLAVEIPVVMVTSTNADRLRDAMGIKSVYIPGLG 193
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BLAST of mRNA_L-elsbetiae_contig2034.6117.1 vs. uniprot
Match: A0A6G0W7Z3_9STRA (PA domain-containing protein n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0W7Z3_9STRA)

HSP 1 Score: 105 bits (263), Expect = 1.150e-24
Identity = 62/150 (41.33%), Postives = 83/150 (55.33%), Query Frame = 1 
Query:  136 LHFCLGCLDAAAEGSLVQALAPHHGIRDFKVSGDLVYAVPNKGNADVLLNGDDMAASIVLFERGEVPVVEKVLKAQAAGAAGIVVVDDGGCSDGLVDCGRLGGVRDGGFAGRDGAHAWLTVKIPVVLISAAQGERLREMMPLQKVAVKGL 585
            L FC  C D A+ GS V      +   D+ V G++ YAVPN  N D L N   +   IVL +RG+VP++EKV + Q AG   +V+VDDG CSDG  +CG LG      F  +D +  W  + IPVVLI    G R++  M L ++ V  L
Sbjct:   46 LTFCKKCHDVASLGSFVHTWDSRYNPSDYHVQGEIAYAVPNTANDD-LFNIHQLEGRIVLVDRGDVPILEKVERVQEAGGIAVVLVDDGECSDGF-ECGSLGSRSTHNFLEQDDSFRWNHIVIPVVLILHDDGMRIKARMSLVRMDVPDL 193
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BLAST of mRNA_L-elsbetiae_contig2034.6117.1 vs. uniprot
Match: A0A7S2WGD9_9STRA (Hypothetical protein (Fragment) n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2WGD9_9STRA)

HSP 1 Score: 89.4 bits (220), Expect = 3.790e-19
Identity = 42/97 (43.30%), Postives = 61/97 (62.89%), Query Frame = 1 
Query:  127 LLHLHFCLGCLDAAAEGSLVQALAPHHGIRDFKVSGDLVYAVPNKGNADVLLNGDDMAASIVLFERGEVPVVEKVLKAQAAGAAGIVVVDDGGCSDG 417
            L  L FC  C D   EG+ V  L    G +++ V+GD+ YAVPN+     LLN  +++  I   +RG++P+VEK+L AQ AGA G+V+ DDG C++G
Sbjct:   55 LRSLEFCRSCPDPHLEGTQVLGLGAAQGPKEYHVTGDMAYAVPNQMQQGALLNSMELSGKIAFVDRGDIPLVEKILLAQDAGAVGVVIADDGNCAEG 151
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BLAST of mRNA_L-elsbetiae_contig2034.6117.1 vs. uniprot
Match: A0A5D6YDE3_9STRA (Proline--tRNA ligase n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6YDE3_9STRA)

HSP 1 Score: 88.2 bits (217), Expect = 9.440e-17
Identity = 61/168 (36.31%), Postives = 77/168 (45.83%), Query Frame = 1 
Query:  133 HLHFCLGCLDAAAEGSLVQALAPHHGIRDFKVSGDLVYAVPNKGNADVLLNGDDMAASIVLFERGEVPVVEKVLKAQAAGAAGIVVVDDGGCSDGLVDCGRLGGVR-----DGGFAGRDGAHA-------------WLTVKIPVVLISAAQGERLREMMPLQKVAVKG 582
            H+ FC  C D   EGS+V           F V+  LV+A+PN G+   L N  ++   I +F RG+V VV K   AQAAGA   +V D   C  G + C R  GV      D     R+ AH+             WL V IPV ++SA  G RLR  M    V V G
Sbjct:  683 HVLFCRDCADLLLEGSVVIPHQSPFNPTKFFVTSALVFAIPNDGS-HALYNAHELQGKIAVFTRGKVSVVHKARVAQAAGAVAALVFDSDQCDPGAMHCHRTVGVSPLVGDDNNDRSRNAAHSRRIGPFHDDPPSEWLPVTIPVAMVSADDGARLRSFMDYVAVEVNG 849
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BLAST of mRNA_L-elsbetiae_contig2034.6117.1 vs. uniprot
Match: D8LI37_ECTSI (PA domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LI37_ECTSI)

HSP 1 Score: 76.3 bits (186), Expect = 2.220e-13
Identity = 45/114 (39.47%), Postives = 61/114 (53.51%), Query Frame = 1 
Query:  202 HHGIRDFKVSGDLVYAVPNKGNADVLLNGDDMAASIVLFERGEVPVVEKVLKAQAAGAAGIVVVDDGGCSDGLVDCGRLGGVRDGGFAGRDGAHAWLTVKIPVVLISAAQGERL 543
            H   R FKV  ++V A P    ++ L NG  +   IV+ +RG++P+V KVL AQ AGA G++V DDG C          G     G+A  D    WL V++PVVL+     ERL
Sbjct:   74 HFSPRSFKVKSEVVVAQPADARSE-LENGRFVRNRIVVVQRGDIPIVHKVLAAQRAGALGVIVADDGRCGGSFGQLCVYGSDPPEGWAALDMPRPWLDVRVPVVLMLREDAERL 186
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig2034.6117.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 7
Match NameE-valueIdentityDescription
D7FPJ4_ECTSI1.170e-4875.96Vacuolar sorting receptor, putative (ISS) n=1 Tax=... [more]
A0A835Z4S0_9STRA1.280e-4650.31PA domain-containing protein n=1 Tax=Tribonema min... [more]
A0A482SJ43_9ARCH6.400e-3439.38PA domain-containing protein n=1 Tax=archaeon TaxI... [more]
A0A6G0W7Z3_9STRA1.150e-2441.33PA domain-containing protein n=1 Tax=Aphanomyces e... [more]
A0A7S2WGD9_9STRA3.790e-1943.30Hypothetical protein (Fragment) n=1 Tax=Rhizochrom... [more]
A0A5D6YDE3_9STRA9.440e-1736.31Proline--tRNA ligase n=1 Tax=Pythium brassicum Tax... [more]
D8LI37_ECTSI2.220e-1339.47PA domain-containing protein n=1 Tax=Ectocarpus si... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig2034contigL-elsbetiae_contig2034:1604..4165 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score75.5
Seed ortholog evalue3.2e-11
Seed eggNOG ortholog2880.D8LI37
Hectar predicted targeting categoryother localisation
EggNOG OGs29K29@1,2RTB6@2759
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Exons4
Model size609
Cds size609
Stop1
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig2034.6117.1prot_L-elsbetiae_contig2034.6117.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig2034 1604..4165 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815839.8870814-CDS-L-elsbetiae_contig2034:1603..17871622815839.8870814-CDS-L-elsbetiae_contig2034:1603..1787Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig2034 1604..1787 +
1691683137.9848597-CDS-L-elsbetiae_contig2034:1603..17871691683137.9848597-CDS-L-elsbetiae_contig2034:1603..1787Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig2034 1604..1787 +
1622815839.897641-CDS-L-elsbetiae_contig2034:2137..21881622815839.897641-CDS-L-elsbetiae_contig2034:2137..2188Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig2034 2138..2188 +
1691683137.997433-CDS-L-elsbetiae_contig2034:2137..21881691683137.997433-CDS-L-elsbetiae_contig2034:2137..2188Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig2034 2138..2188 +
1622815839.9074047-CDS-L-elsbetiae_contig2034:3544..36391622815839.9074047-CDS-L-elsbetiae_contig2034:3544..3639Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig2034 3545..3639 +
1691683138.00852-CDS-L-elsbetiae_contig2034:3544..36391691683138.00852-CDS-L-elsbetiae_contig2034:3544..3639Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig2034 3545..3639 +
1622815839.9192836-CDS-L-elsbetiae_contig2034:3886..41651622815839.9192836-CDS-L-elsbetiae_contig2034:3886..4165Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig2034 3887..4165 +
1691683138.0181205-CDS-L-elsbetiae_contig2034:3886..41651691683138.0181205-CDS-L-elsbetiae_contig2034:3886..4165Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig2034 3887..4165 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig2034.6117.1

>prot_L-elsbetiae_contig2034.6117.1 ID=prot_L-elsbetiae_contig2034.6117.1|Name=mRNA_L-elsbetiae_contig2034.6117.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=203bp
SSSSTSSSSSTSTSTSTSTRRDPQGKIRAAWAGSSRVDAPFPLLHLHFCL
GCLDAAAEGSLVQALAPHHGIRDFKVSGDLVYAVPNKGNADVLLNGDDMA
ASIVLFERGEVPVVEKVLKAQAAGAAGIVVVDDGGCSDGLVDCGRLGGVR
DGGFAGRDGAHAWLTVKIPVVLISAAQGERLREMMPLQKVAVKGLGEQLV
ER*
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mRNA from alignment at L-elsbetiae_contig2034:1604..4165+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig2034.6117.1 ID=mRNA_L-elsbetiae_contig2034.6117.1|Name=mRNA_L-elsbetiae_contig2034.6117.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=2562bp|location=Sequence derived from alignment at L-elsbetiae_contig2034:1604..4165+ (Laminarionema elsbetiae ELsaHSoW15)
AGCAGCAGCAGCACCAGCAGCAGCAGCAGCACCAGCACCAGCACCAGCAC
CAGCACCAGGAGAGACCCACAGGGAAAGATACGTGCCGCGTGGGCAGGAT
CTTCAAGAGTCGACGCTCCTTTCCCGCTATTGCATCTGCATTTCTGTCTG
GGCTGCTTGGATGCCGCTGCAGAAGGCTCACTGGGTACGGAAAACACAAA
AATGTATGCGCGTGTGTACGGCCTTTGCCGTACAGACCTTACATACCGCT
GAATATATCAACAAACATACATACAGCCGAGGCACACAAACTCTGGCTGT
TGTCTGTTGACGTTCGCTTGTTGAGGGGTATCCACAGGAATATCCACTCG
GTGTATCGCAACTTAGTGGTGCTCGGATCTACGTAGCTCAATGCGGGTTC
CCAGCTTGTTCGTATAGATATTCTCTGGTGATGCACAGCAGCACAACCAA
CGAGTTCTAAGGCATTCTTGACCGCAACGCCTGCTATTTTTCCGCACGTT
CCGCTGTGACTGTAACTAAATCACCTCCTGCTAGTCCAAGCTCTTGCACC
GCATCATGGGATTCGAGACTTCAAGGTCTCAGGAGGTGAGATCCTACATT
TTACTCGTGTATTCATCTTAACTCGTGTAATTATTATTAGAAGGATATCA
TGTGTATTTTAACCTAGATATACAAAAAGAAAGAAAAAACCCCAGTGTTC
ACCTCCTCCTGTGTGACCCCGGTTGATTGTAGTTGTACAGAGAAATGCAC
TGCAAAGTGTAGTACCTCTAAGTACACCGCAAGGTGTAGTACACGTCTCC
ACTTGGGGGGACTGTCGCCAGAATAAATGGAAAGGACACACACAAACATA
GGTCCTTCGGCCTCTCGCTCAGGCGCCTAGAGCCTACTTTTTTGGCATTC
TCGGTCCGTCGACCTCGCCCCGCTGGAATCTTTGCGCCTGCCGGTAGCCC
TCGGCTGCGAGGGCCCTGCCCGTGTGCCCTCGCCGTGGGGGCGGCCCGCC
GCGCAGCGGTATTAGCGACCCTTGAAGTCGCGTCTCCGAAATCTACGACG
CCTCGGCTTATCAGCGGCCGACAACGATGACGACAGTAGTCCCGTTGGCG
GGAAAAATTCTTCACACACTAACCATCGCCTACGGGACTAAGCGTAGCGG
TGGCACGCATCAGCGGGCCGCCGCGAAGGGCCCTTGTATTATCTATATCT
TTGGGCCGTAGATTGCGATACTCTCTAGTCTACATACCTTCATCCTCTCG
AAATATCGCCCCGCTCTTTTGCTCCGTCTTATTTCCCCCTTTTGGTACGA
TAGCGCGGTGATTTTATCACTGTGCCATAACATAAAGCTGCGAAAAACGT
CGGTTGTTATGTACCAGATATATGTGTATACGGGGCTTATACTTCGGTTT
TTCCAAATGGGACTTTAATTTCGGAACAGACCGGTAGTCGATTAGACTAA
TGGCATGCACAGCGCGGCAGTAGACTTGGTTTATACTGCACCGTAACAAA
TCTTATGACGATCATACGGGGCATTAAAGCCTGCCACCAGCCCCGACCCG
CCCCCGAAATTGTCTGTCTCGGCCTTTTGTTTACCGTCGAGCACATCCCG
AAGACCCAACAACCGGCTTTGGTCGCTTCGTCGTCGGCGTCCTCGTCGCA
CTCAAAACTCGACACTCAAAACTCGATACTCGAAGCTCGACACTCGAAGC
TCGACACTCGAAACTCGAGTATTTTTCCTTGCGCCCCAGAGACTATTTCC
GCTCCCACTTTGCTATGCATCCCATTCGCATCAAGCACAGCCTTGAAGTG
ACCATGACTGTGGCGCTGGTCAATCAGCGTGGATCACTCTTCTCGTACTC
CGGGGGCTCTTTCGCCTCGCTTACTGCAACATGCACCTTCGCGCCTTGTA
TCGTCGCCGCCCATCATACTCTCGTCCGGCTGCGAGAAAAGACCTGGTGT
ACGCGGTGCCAAACAAGGGCAACGCGGACGTGCTTCTAAACGGCGACGAT
ATGGCCGCAAGCATAGTCCTGTTCGAGAGAGGAGAGGTGAGGAGCGGGCA
GGGACACGAGCCTCACCGACTGCGTGACAAAGCTTCACATCTATCTGCGG
TCCGGCAACTCGCGAAACGAAACTGCAATTGAGCTTTCTACAACGACATG
CCGGCTCAGGCTATGTAGCCTGCCTGTGTAGCGTACCAAAAGCGCATCCG
CACGCAAGTAGCGAAGCGCAACACACACGCCGTGCCCTTCGCGCGACTCC
CCTTCTTCGTATCCACGGCCTTGACATGCACAGGTTCCGGTGGTGGAAAA
GGTCCTCAAGGCCCAGGCGGCGGGGGCGGCCGGCATCGTTGTCGTCGACG
ATGGGGGCTGTAGCGACGGGCTCGTCGACTGTGGCCGACTTGGCGGTGTG
CGAGACGGTGGGTTCGCCGGCCGGGACGGCGCACACGCGTGGCTTACCGT
GAAGATCCCGGTGGTGCTGATTTCGGCCGCCCAGGGGGAGAGGCTTCGAG
AGATGATGCCCCTGCAGAAGGTTGCGGTGAAAGGATTGGGGGAGCAGCTT
GTGGAGCGGTAG
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Coding sequence (CDS) from alignment at L-elsbetiae_contig2034:1604..4165+

>mRNA_L-elsbetiae_contig2034.6117.1 ID=mRNA_L-elsbetiae_contig2034.6117.1|Name=mRNA_L-elsbetiae_contig2034.6117.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=1218bp|location=Sequence derived from alignment at L-elsbetiae_contig2034:1604..4165+ (Laminarionema elsbetiae ELsaHSoW15)
AGCAGCAGCAGCACCAGCAGCAGCAGCAGCACCAGCACCAGCACCAGCAC
CAGCACCAGGAGAGACCCACAGGGAAAGATACGTGCCGCGTGGGCAGGAT
CTTCAAGAGTCGACGCTCCTTTCCCGCTATTGCATCTGCATTTCTGTCTG
GGCTGCTTGGATGCCGCTGCAGAAGGCTCACTGGAGCAGCAGCAGCACCA
GCAGCAGCAGCAGCACCAGCACCAGCACCAGCACCAGCACCAGGAGAGAC
CCACAGGGAAAGATACGTGCCGCGTGGGCAGGATCTTCAAGAGTCGACGC
TCCTTTCCCGCTATTGCATCTGCATTTCTGTCTGGGCTGCTTGGATGCCG
CTGCAGAAGGCTCACTGGTCCAAGCTCTTGCACCGCATCATGGGATTCGA
GACTTCAAGGTCTCAGGAGTCCAAGCTCTTGCACCGCATCATGGGATTCG
AGACTTCAAGGTCTCAGGAGACCTGGTGTACGCGGTGCCAAACAAGGGCA
ACGCGGACGTGCTTCTAAACGGCGACGATATGGCCGCAAGCATAGTCCTG
TTCGAGAGAGGAGAGACCTGGTGTACGCGGTGCCAAACAAGGGCAACGCG
GACGTGCTTCTAAACGGCGACGATATGGCCGCAAGCATAGTCCTGTTCGA
GAGAGGAGAGGTTCCGGTGGTGGAAAAGGTCCTCAAGGCCCAGGCGGCGG
GGGCGGCCGGCATCGTTGTCGTCGACGATGGGGGCTGTAGCGACGGGCTC
GTCGACTGTGGCCGACTTGGCGGTGTGCGAGACGGTGGGTTCGCCGGCCG
GGACGGCGCACACGCGTGGCTTACCGTGAAGATCCCGGTGGTGCTGATTT
CGGCCGCCCAGGGGGAGAGGCTTCGAGAGATGATGCCCCTGCAGAAGGTT
GCGGTGAAAGGATTGGGGGAGCAGCTTGTGGAGCGGTAGGTTCCGGTGGT
GGAAAAGGTCCTCAAGGCCCAGGCGGCGGGGGCGGCCGGCATCGTTGTCG
TCGACGATGGGGGCTGTAGCGACGGGCTCGTCGACTGTGGCCGACTTGGC
GGTGTGCGAGACGGTGGGTTCGCCGGCCGGGACGGCGCACACGCGTGGCT
TACCGTGAAGATCCCGGTGGTGCTGATTTCGGCCGCCCAGGGGGAGAGGC
TTCGAGAGATGATGCCCCTGCAGAAGGTTGCGGTGAAAGGATTGGGGGAG
CAGCTTGTGGAGCGGTAG
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