mRNA_L-elsbetiae_contig12957.2342.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

BLAST of mRNA_L-elsbetiae_contig12957.2342.1 vs. uniprot
Match: A0A7Y4R9F3_9BACT (Carbon-nitrogen hydrolase family protein n=1 Tax=Phycisphaerales bacterium TaxID=2052180 RepID=A0A7Y4R9F3_9BACT)

HSP 1 Score: 406 bits (1043), Expect = 1.700e-139
Identity = 198/313 (63.26%), Postives = 237/313 (75.72%), Query Frame = 1

Query:   25 LKVAIAQLAPVLLNREATVARAAGVVAEAGRAGAGLVAFGETFVPAYPVWLCRMDAARFDAADVKAMHAVYLDQAVDLEAGHLSPVREAARDANCKVVMGIAERPRDRGSHSLYCTAVVIAEDGSIASAHRKLMPTYEERLSWSIGDGHGLVTHPCGPFTLGALNCWENWMPLARASLYAQGENLHIALWPGCLRLTGEITRFIAKEGRSFVISASSIIRASDVPATLPHRAEWVSDEAETFYDGGSCIAGPDGEWIVEPVVGTEGLIFADLDVRRVYEERQNFDPSGHYARPDVLNLNVDRTRQAATKFRDA 963
            +K+AIAQ+APV LNR+AT  +    + EA   G  LVAFGET +PAYP W+ R D ARFDA D K  +A+YLDQAV+++AGHL PV +AAR  N  V++GIAERPRDRG HS+YC+ V +   G +AS HRKLMPT+EERL+W IGDG GLVTH  G FT+GALNCWENWMPLARA+LYA GE+LH+ LWPG LRLT +ITR++A EGRSFV+SAS+I+R  D+PA LP R + ++   ET Y+GGSC+AGPDG WIVEPVV  E LI ADLD   V  ERQ FDP GHYARPDVL L VDR RQ    + DA
Sbjct:    1 MKIAIAQIAPVFLNRDATTRKVVDHIHEAADVGCQLVAFGETLIPAYPAWISRTDGARFDADDQKEWYALYLDQAVNIDAGHLHPVCDAARKRNIAVIVGIAERPRDRGGHSIYCSRVFVDHTGRVASIHRKLMPTHEERLAWGIGDGAGLVTHRVGEFTVGALNCWENWMPLARAALYAAGEDLHVMLWPGDLRLTRDITRYVALEGRSFVVSASAILRDRDLPADLPQRDQ-LAAPGETLYNGGSCVAGPDGRWIVEPVVEREALITADLDPGMVRRERQTFDPVGHYARPDVLRLTVDRRRQTPAHWLDA 312          

BLAST of mRNA_L-elsbetiae_contig12957.2342.1 vs. uniprot
Match: A0A7Y5RVK8_9BACT (Carbon-nitrogen hydrolase family protein n=1 Tax=Phycisphaerae bacterium TaxID=2026778 RepID=A0A7Y5RVK8_9BACT)

HSP 1 Score: 399 bits (1024), Expect = 1.270e-136
Identity = 196/311 (63.02%), Postives = 228/311 (73.31%), Query Frame = 1

Query:   28 KVAIAQLAPVLLNREATVARAAGVVAEAGRAGAGLVAFGETFVPAYPVWLCRMDAARFDAADVKAMHAVYLDQAVDLEAGHLSPVREAARDANCKVVMGIAERPRDRGSHSLYCTAVVIAEDGSIASAHRKLMPTYEERLSWSIGDGHGLVTHPCGPFTLGALNCWENWMPLARASLYAQGENLHIALWPGCLRLTGEITRFIAKEGRSFVISASSIIRASDVPATLPHRAEWVSDEAETFYDGGSCIAGPDGEWIVEPVVGTEGLIFADLDVRRVYEERQNFDPSGHYARPDVLNLNVDRTRQAATKFRD 960
            +VAI Q+A   LNREA  AR    +  A  AG  LVAFGE  +PAYP+W+CR D ARFD A+ KA+HA+YLDQAV  + GHL P+ +AAR     VV+GIAER  DRG HSL+C+ VVI   G   S HRKLMPTYEERL+W IGDG GLVTH  GPF LGALNCWENW+PLARA+LYA GE+LH+ LWPG   LT +ITRF+A E RSFV+SAS+I+R SD+PA +P R E ++   E   DGGSCIAGPDG W+VEPVVG E L+ ADLD  RV EER NFDP+GHYARPDVL L VDR R  A  F D
Sbjct:    3 RVAIGQIASAFLNREAATARVVAAINRAAEAGCQLVAFGEALIPAYPLWICRTDGARFDNAEQKALHALYLDQAVAPDRGHLDPISDAARRGRIAVVLGIAERAADRGGHSLFCSRVVIDGGGKTLSIHRKLMPTYEERLAWGIGDGAGLVTHAIGPFMLGALNCWENWLPLARAALYAAGEDLHVMLWPGSANLTRDITRFVAMESRSFVLSASAIVRESDLPADMPLR-ERIAQPGEMLCDGGSCIAGPDGRWMVEPVVGREELLVADLDPARVREERHNFDPAGHYARPDVLRLIVDRRRLRAADFLD 312          

BLAST of mRNA_L-elsbetiae_contig12957.2342.1 vs. uniprot
Match: A0A5Q4ERV8_9BACT (Carbon-nitrogen hydrolase family protein n=1 Tax=Phycisphaeraceae bacterium TaxID=2026777 RepID=A0A5Q4ERV8_9BACT)

HSP 1 Score: 394 bits (1012), Expect = 7.330e-135
Identity = 196/312 (62.82%), Postives = 233/312 (74.68%), Query Frame = 1

Query:   25 LKVAIAQLAPVLLNREATVARAAGVVAEAGRAGAGLVAFGETFVPAYPVWLCRMDAARFDAADVKAMHAVYLDQAVDLEAGHLSPVREAARDANCKVVMGIAERPRDRGSHSLYCTAVVIAEDGSIASAHRKLMPTYEERLSWSIGDGHGLVTHPCGPFTLGALNCWENWMPLARASLYAQGENLHIALWPGCLRLTGEITRFIAKEGRSFVISASSIIRASDVPATLPHRAEWVSDEAETFYDGGSCIAGPDGEWIVEPVVGTEGLIFADLDVRRVYEERQNFDPSGHYARPDVLNLNVDRTRQAATKFRD 960
            +++AIAQ+APV L+R AT  +AA  + +A    A LV FGET VPAYP+WL R D ARF++   K  HA YLDQAVDLEAG L+ V+ AA+     VV+G+AERPRDRG H+++C+ V I E G+IAS HRKLMPTYEERL+W +GDG GLVTH    FT+GALNCWENW+PLARA+L+AQGE+LHIALWPG  RLT + TRF A EGRSFVISAS IIR  D+PA LP R   ++ + E  Y GGSC+A PDG W+VEPVV  E LI ADLD   V  ERQNFDP+GHY RPDVL L VDR RQAA +F D
Sbjct:    1 MRIAIAQIAPVFLDRAATCEKAAQWIRDAAAENARLVCFGETLVPAYPLWLDRTDGARFESDLQKQFHARYLDQAVDLEAGDLATVQAAAKKHGVNVVIGVAERPRDRGGHTIFCSRVFINETGAIASVHRKLMPTYEERLAWGVGDGAGLVTHRIDEFTVGALNCWENWIPLARAALHAQGEDLHIALWPGAERLTRDSTRFFALEGRSFVISASCIIREQDLPADLPERIR-IAQKGEVLYSGGSCVAAPDGSWLVEPVVNEERLIIADLDHAHVRRERQNFDPAGHYTRPDVLRLTVDRRRQAAAEFLD 311          

BLAST of mRNA_L-elsbetiae_contig12957.2342.1 vs. uniprot
Match: A0A7Y2HXT3_9BACT (Carbon-nitrogen hydrolase family protein n=1 Tax=Phycisphaerales bacterium TaxID=2052180 RepID=A0A7Y2HXT3_9BACT)

HSP 1 Score: 393 bits (1010), Expect = 2.960e-134
Identity = 192/314 (61.15%), Postives = 227/314 (72.29%), Query Frame = 1

Query:   13 DAGTLKVAIAQLAPVLLNREATVARAAGVVAEAGRAGAGLVAFGETFVPAYPVWLCRMDAARFDAADVKAMHAVYLDQAVDLEAGHLSPVREAARDANCKVVMGIAERPRDRGSHSLYCTAVVIAEDGSIASAHRKLMPTYEERLSWSIGDGHGLVTHPCGPFTLGALNCWENWMPLARASLYAQGENLHIALWPGCLRLTGEITRFIAKEGRSFVISASSIIRASDVPATLPHRAEWVSDEAETFYDGGSCIAGPDGEWIVEPVVGTEGLIFADLDVRRVYEERQNFDPSGHYARPDVLNLNVDRTRQAATKF 954
            D   L+V +AQ+A V+L+R AT+ +    V EA R G GLV FGET +P YP W+ R D ARFDAAD KA+HA YL  AV  EAG L+ +   A      V++G+AER  DRG HSLYC+ V I  DG+IAS HRKLMPTYEERLSWSIGDG GLV HP GPF +G LNC+ENWMPLAR +L  QGE+LH+ALWPG  RLTGEITRF+A+E RS+VIS S ++RA D PAT PHR      + E  +DGGS +AGPDG+W+V PV G E LI  DLD  RV EERQNFDP+GHYARPDVL L VDRTRQ+  + 
Sbjct:    3 DPRRLRVGLAQIAAVVLDRGATMNKVVARVEEAAREGCGLVVFGETVLPGYPAWIARTDGARFDAADQKALHAAYLRAAVQPEAGDLADLCACAARHRIAVMIGVAERAADRGGHSLYCSRVFITADGAIASTHRKLMPTYEERLSWSIGDGAGLVVHPVGPFRVGGLNCYENWMPLARVALQGQGEDLHVALWPGAERLTGEITRFLAREARSYVISISGLMRADDFPATFPHRERLQLKDGEVLFDGGSAVAGPDGDWVVPPVCGRETLIVVDLDHDRVLEERQNFDPAGHYARPDVLRLVVDRTRQSGVEL 316          

BLAST of mRNA_L-elsbetiae_contig12957.2342.1 vs. uniprot
Match: Q6RWL2_9ZZZZ (Nitrilase n=1 Tax=uncultured organism TaxID=155900 RepID=Q6RWL2_9ZZZZ)

HSP 1 Score: 392 bits (1007), Expect = 5.020e-134
Identity = 188/312 (60.26%), Postives = 231/312 (74.04%), Query Frame = 1

Query:   25 LKVAIAQLAPVLLNREATVARAAGVVAEAGRAGAGLVAFGETFVPAYPVWLCRMDAARFDAADVKAMHAVYLDQAVDLEAGHLSPVREAARDANCKVVMGIAERPRDRGSHSLYCTAVVIAEDGSIASAHRKLMPTYEERLSWSIGDGHGLVTHPCGPFTLGALNCWENWMPLARASLYAQGENLHIALWPGCLRLTGEITRFIAKEGRSFVISASSIIRASDVPATLPHRAEWVSDEAETFYDGGSCIAGPDGEWIVEPVVGTEGLIFADLDVRRVYEERQNFDPSGHYARPDVLNLNVDRTRQAATKFRD 960
            LK+A AQ+ PV L+R AT  +    + +A   GA LVAFGE  +PAYP+WL R DAARF++   K +HA+YL Q+V +  GHLSP+ + A +    V++GIAER  DRG H++YC+ V I  DG IAS HRKLMPTYEERLSW  GDG GLVTHP GPFT+GALNCWENWMPLAR +LYA GE+LH+A+WPG   LT +ITRFIA+E RSFV+S S IIR SD+P+ +P+R E  + + ET Y+GGSCIAGPDG+WI+ PV   E LIFA++D   V  ERQNFDP+GHYARPDVL + VDR RQ A  F D
Sbjct:    5 LKIAAAQITPVFLDRVATTKKVVETIEKAAAGGARLVAFGEALLPAYPLWLTRTDAARFNSDVQKNLHAIYLKQSVSIAGGHLSPICKIASERKIAVILGIAERATDRGDHTIYCSCVFIDADGRIASVHRKLMPTYEERLSWGFGDGAGLVTHPVGPFTVGALNCWENWMPLARTALYAGGEDLHVAIWPGGSVLTEDITRFIARESRSFVLSVSGIIRESDIPSGVPYRDEMCA-KGETIYNGGSCIAGPDGQWIIAPVTDREELIFAEIDHEHVRRERQNFDPAGHYARPDVLQITVDRRRQTAANFID 315          

BLAST of mRNA_L-elsbetiae_contig12957.2342.1 vs. uniprot
Match: A0A3M8FR53_9BACT (Carbon-nitrogen hydrolase family protein n=1 Tax=Phycisphaera sp. TaxID=2030824 RepID=A0A3M8FR53_9BACT)

HSP 1 Score: 378 bits (970), Expect = 2.250e-128
Identity = 186/318 (58.49%), Postives = 236/318 (74.21%), Query Frame = 1

Query:   25 LKVAIAQLAPVLLNREATVARAAGVVAEAGRAGAGLVAFGETFVPAYPVWLCRMDAARFDAADVKAMHAVYLDQAVDLEAGHLSPVREAARDANCKVVMGIAERPRDRGSHSLYCTAVVIAEDGSIASAHRKLMPTYEERLSWSIGDGHGLVTHP-CGPFTLGALNCWENWMPLARASLYAQGENLHIALWPGCLRLTGEITRFIAKEGRSFVISASSIIRASDVPATLPHRAEWVSDEAET-----FYDGGSCIAGPDGEWIVEPVVGTEGLIFADLDVRRVYEERQNFDPSGHYARPDVLNLNVDRTRQAATKFRD 960
            + +AIAQ+APV L+R AT+ +AA  + EA  +GA LVAFGETF P YP WL R D+ARFDA D K +HA YL +A+D+EAG L+PV EAAR+ +  VV+GIAERP DRGSHS++ + V I+  G +AS HRKLMPTYEERL W+ GDG+GL TH    PFTLG+LNCWENWMPL RA+LYAQG +LH+A+WPG L  T +ITRFIA+E RS+VISAS+I+R  D+P   P R   + +  ET      ++GGSCIA PDG W+VEP+   E +I A++D  RV EERQNFDP+GHY+RP+VL L++DR R +A    D
Sbjct:    1 MHIAIAQIAPVPLDRAATLNKAASAIREASASGAKLVAFGETFAPCYPFWLSRTDSARFDATDQKHLHARYLQEAIDIEAGDLAPVTEAARETDTAVVIGIAERPADRGSHSVFASCVTISPTGEVASVHRKLMPTYEERLCWTPGDGNGLRTHSFLPPFTLGSLNCWENWMPLPRAALYAQGLDLHVAIWPGSLANTQDITRFIARESRSYVISASTILRPMDIPQDFPERQR-ILESTETPEDGFIHNGGSCIAAPDGSWLVEPITHREQIITAEIDHARVLEERQNFDPAGHYSRPEVLKLSLDRARHSAIDIND 317          

BLAST of mRNA_L-elsbetiae_contig12957.2342.1 vs. uniprot
Match: UPI001AD5832D (Nitrilase n=1 Tax=Phycisphaerales bacterium TaxID=2052180 RepID=UPI001AD5832D)

HSP 1 Score: 371 bits (952), Expect = 1.020e-125
Identity = 185/314 (58.92%), Postives = 224/314 (71.34%), Query Frame = 1

Query:   25 LKVAIAQLAPVLLNREATVARAAGVVAEAGRAGAGLVAFGETFVPAYPVWLCRMDAARFDAADVKAMHAVYLDQAVDLEAGHLSPVREAARDANCKVVMGIAERPRDRGSHSLYCTAVVI--AEDGSIASAHRKLMPTYEERLSWSIGDGHGLVTHPCGPFTLGALNCWENWMPLARASLYAQGENLHIALWPGCLRLTGEITRFIAKEGRSFVISASSIIRASDVPATLPHRAEWVSDEAETFYDGGSCIAGPDGEWIVEPVVGTEGLIFADLDVRRVYEERQNFDPSGHYARPDVLNLNVDRTRQAATKFRD 960
            +KVA+AQ+ PVLL+R+AT+A+    V  A   GA LV F E   P YPVW+ R D ARFD+A  K + A+Y +QAV +E G L P   A RDA    V+GI ERPRDR  HSLY + V++   +DG I S HRKLMPTYEERL W IGDGHGL  HP G FTLGALNCWENWMPLARA+LYAQG +LH+ALWPG  R T +ITRFIA E RS+V+S   +IRASDVPA+ P R+ +     ET+ +GGSC+A PDG W++EPV   E ++FA++D R V   RQNFDP+GHYARPDVL L VDR RQA  +F D
Sbjct:    1 MKVALAQIVPVLLDRDATLAKCIDAVRAARAGGASLVCFPEALAPGYPVWVERTDGARFDSAVQKDLFALYAEQAVCIERGDLLPFCAACRDAGVMGVLGIIERPRDRTGHSLYASRVIVDGRQDGRILSVHRKLMPTYEERLVWGIGDGHGLTAHPIGDFTLGALNCWENWMPLARAALYAQGVDLHVALWPGSDRNTRDITRFIAMESRSYVVSVGGLIRASDVPASTPARSVFAGAN-ETYCNGGSCVAAPDGSWVIEPVTNREEVLFAEIDHREVRRARQNFDPAGHYARPDVLRLVVDRRRQATAEFLD 313          

BLAST of mRNA_L-elsbetiae_contig12957.2342.1 vs. uniprot
Match: A0A545TBU8_9GAMM (Carbon-nitrogen hydrolase family protein n=1 Tax=Aliikangiella marina TaxID=1712262 RepID=A0A545TBU8_9GAMM)

HSP 1 Score: 370 bits (951), Expect = 1.610e-125
Identity = 181/313 (57.83%), Postives = 219/313 (69.97%), Query Frame = 1

Query:   22 TLKVAIAQLAPVLLNREATVARAAGVVAEAGRAGAGLVAFGETFVPAYPVWLCRMDAARFDAADVKAMHAVYLDQAVDLEAGHLSPVREAARDANCKVVMGIAERPRDRGSHSLYCTAVVIAEDGSIASAHRKLMPTYEERLSWSIGDGHGLVTHPCGPFTLGALNCWENWMPLARASLYAQGENLHIALWPGCLRLTGEITRFIAKEGRSFVISASSIIRASDVPATLPHRAEWVSDEAETFYDGGSCIAGPDGEWIVEPVVGTEGLIFADLDVRRVYEERQNFDPSGHYARPDVLNLNVDRTRQAATKFRD 960
            T+K A+AQ++PVLLNREAT+ + A  V +A   G  LVAFGE  VP YP WL R D ARF+    K + A Y  QAV +E G L P+ E A+  N  + +GI ERP DRG HSLYC+ V I+E G IAS HRKLMPTYEERLSWS GDGHGLVTH  G FT+G LNCWENWMPLARASLYAQGE+LH+++WPG L+ T ++T  +AKEGRS+ ++   ++R +DV  TLP  AE    +   F +GGSCIA PDG W++EP    EGL  A+LD   V  ER NFDPSGHY+RPDV  L VDR RQA   F+D
Sbjct:    3 TIKAALAQISPVLLNREATLLKVAQYVHDAADKGCQLVAFGEALVPGYPFWLERTDGARFNDDKQKKIQAEYAKQAVQIERGDLEPLCEVAKQKNIAIYLGIIERPSDRGGHSLYCSMVYISEKGQIASVHRKLMPTYEERLSWSPGDGHGLVTHSLGEFTVGGLNCWENWMPLARASLYAQGEDLHVSIWPGNLKNTYDLTSVLAKEGRSYHMAVCGLMRPTDVDVTLPELAELADADEAFFANGGSCIANPDGSWLIEPQTECEGLFIAELDHASVRRERHNFDPSGHYSRPDVTQLTVDRRRQATAVFKD 315          

BLAST of mRNA_L-elsbetiae_contig12957.2342.1 vs. uniprot
Match: UPI00174D29D1 (carbon-nitrogen hydrolase family protein n=1 Tax=Marinomonas colpomeniae TaxID=2774408 RepID=UPI00174D29D1)

HSP 1 Score: 369 bits (946), Expect = 9.910e-125
Identity = 174/312 (55.77%), Postives = 224/312 (71.79%), Query Frame = 1

Query:   25 LKVAIAQLAPVLLNREATVARAAGVVAEAGRAGAGLVAFGETFVPAYPVWLCRMDAARFDAADVKAMHAVYLDQAVDLEAGHLSPVREAARDANCKVVMGIAERPRDRGSHSLYCTAVVIAEDGSIASAHRKLMPTYEERLSWSIGDGHGLVTHPCGPFTLGALNCWENWMPLARASLYAQGENLHIALWPGCLRLTGEITRFIAKEGRSFVISASSIIRASDVPATLPHRAEWVSDEAETFYDGGSCIAGPDGEWIVEPVVGTEGLIFADLDVRRVYEERQNFDPSGHYARPDVLNLNVDRTRQAATKFRD 960
            L+VAIAQ++PV LNR+ T+ +    ++EA   GA LV FGET +P YP W+   + A F++   K +HA YL QAV++E GHL P+ + A+  +  V++G+AER  DRG HS+YC+ V I +DG I + HRKLMPTYEERL+W+ GDGHGL T P GPFT+GALNCWENWMPLARA+LY QGENLH+A+WPG    T +ITRFIAKE RS+VISAS ++RA D PA  P+  E +        +G SCIAGPDGEW+VEP +G EG+  A LD   V EER +FDP GHY+RPDV  L+++R RQ+  K +D
Sbjct:    7 LQVAIAQISPVWLNRDKTIDKILAAISEAANKGAELVTFGETLLPGYPFWIELTNGAEFNSTRQKEIHAHYLRQAVNIEEGHLQPICDLAKQRSIAVIVGVAERATDRGGHSVYCSLVYIDKDGLIQNVHRKLMPTYEERLTWASGDGHGLRTFPVGPFTMGALNCWENWMPLARAALYGQGENLHVAIWPGGEHNTHDITRFIAKESRSYVISASGLMRAEDFPADTPYLEEILEHAKPMLANGASCIAGPDGEWLVEPQIGKEGIYLATLDFNTVLEERHSFDPVGHYSRPDVTQLHINRERQSTIKIKD 318          

BLAST of mRNA_L-elsbetiae_contig12957.2342.1 vs. uniprot
Match: A0A7I7QLX6_9MYCO (Nitrilase n=1 Tax=Mycolicibacterium sediminis TaxID=1286180 RepID=A0A7I7QLX6_9MYCO)

HSP 1 Score: 369 bits (946), Expect = 1.030e-124
Identity = 181/313 (57.83%), Postives = 218/313 (69.65%), Query Frame = 1

Query:   22 TLKVAIAQLAPVLLNREATVARAAGVVAEAGRAGAGLVAFGETFVPAYPVWLCRMDAARFDAADVKAMHAVYLDQAVDLEAGHLSPVREAARDANCKVVMGIAERPRDRGSHSLYCTAVVIAEDGSIASAHRKLMPTYEERLSWSIGDGHGLVTHPCGPFTLGALNCWENWMPLARASLYAQGENLHIALWPGCLRLTGEITRFIAKEGRSFVISASSIIRASDVPATLPHRAEWVSDEAETFYDGGSCIAGPDGEWIVEPVVGTEGLIFADLDVRRVYEERQNFDPSGHYARPDVLNLNVDRTRQAATKFRD 960
            T  V   Q+APV L+REAT+A+  G V EAG  G  LV FGET VP YP WL R D A+F++   + +HA YL +AVD+EAGHL  VR+AAR     +V+G+AERP DRG HS+YC+A+ +   G +AS HRKL+PTYEERLSW+ GDGHGLVTH  GPFT G LNCWENWMPL+R +LYAQGE+LHIALWPG +  T +ITRFIA E RS+VIS S + R  D+P  +P      +   E    GG+CIA PDG W+VEP+   EGL  A LD  RV  ERQNFDP+GHY RPDV +L VDR RQ    F D
Sbjct:    7 TKVVGAVQMAPVFLDREATLAKVEGYVDEAGANGCELVVFGETVVPGYPFWLGRTDGAKFNSDLQRDIHADYLQEAVDIEAGHLDGVRDAARRNGVSLVLGVAERPGDRGGHSIYCSAITVDSRGEVASVHRKLIPTYEERLSWANGDGHGLVTHELGPFTFGTLNCWENWMPLSRTALYAQGEDLHIALWPGDVSDTADITRFIALESRSYVISVSGVYRRDDIPDGMPGADIMRNASDEQISTGGTCIAAPDGTWVVEPIDSREGLTVATLDHGRVRRERQNFDPAGHYGRPDVTSLVVDRRRQTLATFTD 319